MD07G1145200.v1.1

Mitogen-activated protein kinase kinase kinase 7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013904
Physical Location & Seq
Forward (+)
21163304 .. 21165262
1959 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1145200.v1.1.491

Sequence Viewer

Length: 1959 bp
ATGGAACAATTCCGAAGGATTGGGGAGGATGTAGGCAGCTTAAAGGCTTTAATGGTGTTCCAAGACAACATCCAAATCAATCAGCGGCAGTGCTTTTTGCTGCTCGATATCTTCAGCTCAGCATATGAATCAATAGCGGAGGAGATAAGACATAATCTTCGGTTTGATGAGAAGCACATGAAATGGAGGGTTCTCGAGCAGCCTCTGAGAGAGCTCCACAGGATATTCAAAGATGCAGAAGCCTACATTCGACAATGCTTGGATACCCAGGATTGGTGGGCTAAGGCCATCACACTCTATCAGAATTCCGATTGTGTCGAGTTTCACATCCATAACCTACTTGCCTGCGTGCCGATCGTCATCGAAGCAATCGACATTGCTGGAGAAATATCAGGGTTGGATCAGGATGAAATACAGAGGAAGAAAACCATGTTCGCAGACAAGTACAAACAAGACTACAGAGATTGGAAACTTTTCAAGTGGAGATTTGGGAAGCAGTATTTGATTACTCAAGACTTCTGCAATAGGTTTGAATCAGCAGGAAATGAAGATAGATGGACTCTTCTGCATAAAATCAGCGAAAAGAAACTTTCAGGTTCAACAAAGTATGGGAAACGACTCATAGATCTTTTCAAAAGCGCAGACGGATCAGAGTCACAGAATTTGAAAGGGAAGCTCTTACCTAGTTCAGTTCTGGTGGGATCTAAGGACTACCAGGTGAGGCGGCGCCTTGGGGCTGGAAGTCAGTATAAGGAGATCGTATGGTTGGGTGAAACCTTTGCCTCAAGACATTTCTTTGGGGAAATTGAACCCTTGTTGTCGGAGATTTCTTTGTTGTTATCACTTTCCCACCCAAACATAGTGCTCTTCCCTTGTGGCTTTACAGATGAAGAAAAGAAAGAGTGTTTTCTCATAATGGAACTGATGAGTAGAGACCTCTGCAGCTACATCAAAGCGATCTGTGGCCCCCGAAAACGTCTACCATTTTCTCTTCCTGTTGCTATTGATCTGATGCTTCAAATTGCAAGAGGAATGGAATATCTCCACTCAAAAAAAATCTACCACGGAGATTTGGATCCTTCTAATGTACTTGTTAAAGCAAGAGGCATCTCCACAGAAGGTTACTTGCAAGCCAAGGTTTCAGGTTTTGGTTTAGCTTCTGTCAATACCTCCAACCACAAAAACCCTTCAAATCAGAATGGAAGCCTGTCTTTCATCTGGTATGCGCCTGAAGTTCTGGAAGAGCAAGAACAAGGAAAAAGTACTGAGAAAAAGTACGCAGAAAAGTCCGATGTGTATAGCTTTGGAATGGTTTGCTTTGAACTCCTGACAGGGAAAGTCCCTTTTGAGGATAGTCATCTTCAAGGGGACAAGATGAGCCGAAACATAAGGGCCGGGGAGAGGCCGCTGTTTCCATTCTATCATCTGAGATATGTGACCAACCTCACAAAGAAATGCTGGCATAGTGACCCCAATCAACGGCCAAGTTTCTCATCCATTTGTAGGATTCTTCGCTACATTAAACGGTTCCTTGCGATGAATCCTGATTACAACAGCCAGCTAGACCCGCCTGTTCCTATGGTAGATTACTGTGAGATTGAGTCTGGGCTTCTGAGAAAGATTCCTTCCTGGGGGAGTTCTGAACCATCACCAATATCACAAATCCCATTCCAAATGTTCGTTTATAGGATCGCAGAGAGAGAAAGAACGTCTTCAAGCCTGAAGGATACTTCAGAATCTGGAAGTGACGGAGCTTCAATCTGTGGCGATGAAATTGTGGTCCCTGCAGATGATCCGTTCTCAACAACACCTGAAAGGAAGAGTTTATCTTCGCCTGATAGTGTGAAAAAGAAACTTCCATTCTTGAAGAAATCGTCGGATGTGAAATCCAACAGACTACCAGGTTGCCGTAATCATACAACTCATTCATGCCCCATTATTTTTTATACACAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1221 Pkinase_Tyr

Protein Analysis

653

Amino Acids

74.91

Weight (kDa)

8.31

Isoelectric Point (pI)

49.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1221 PF06760 21 - 235 1.7e-100 Protein of unknown function (DUF1221)
PK_Tyr_Ser-Thr PF07714 269 - 503 2e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 271 - 501 6.8e-35 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013904)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01450
fragaria_vesca FvH4_7g15980
malus_domestica MD01G1076300.v1.1 MD07G1145200.v1.1
prunus_persica Prupe.2G183700_v2.0.a1 Prupe.2G183700_v2.0.a1
pyrus_communis pycom07g14470
rosa_chinensis RchiOBHm_Chr1g0358861
rosa_laevigata RLG00000027921
rosa_multiflora Rmu_sc0008757.1_g000014
rosa_roxburghii Rroxscaffold_4G00297570
rosa_rugosa Rorug01G0265700
rosa_samantha Rh1AG279600 Rh1BG246400 Rh1CG263800 Rh1DG274800
rosa_wichuraiana Rw1G024860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 726
AccI GTMKAC 1 cut(s) 979
AciI CCGC 5 cut(s) 85, 137, 724, 1406, 1568
AclWI GGATC 7 cut(s) 408, 655, 709, 1070, 1083, 1697, 1787
AcoI YGGCCR 1 cut(s) 1481
AcsI RAATTY 3 cut(s) 304, 661, 1953
AcuI CTGAAG 4 cut(s) 97, 1251, 1716, 1742
AcyI GRCGYC 1 cut(s) 727
AfaI GTAC 4 cut(s) 446, 1089, 1264, 1277
AfiI CCNNNNNNNGG 6 cut(s) 273, 1348, 1401, 1479, 1503, 1631
AjnI CCWGG 4 cut(s) 267, 714, 1628, 1900
AluBI AGCT 9 cut(s) 39, 117, 214, 676, 945, 1157, 1302, 1561, 1754
AluI AGCT 9 cut(s) 39, 117, 214, 676, 945, 1157, 1302, 1561, 1754
Alw21I GWGCWC 2 cut(s) 216, 867
Alw26I GTCTC 1 cut(s) 927
AlwI GGATC 7 cut(s) 408, 655, 709, 1070, 1083, 1697, 1787
AlwNI CAGNNNCTG 2 cut(s) 205, 1739
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 5 cut(s) 285, 964, 1392, 1403, 1481
ApeKI GCWGC 4 cut(s) 36, 100, 199, 942
ApoI RAATTY 3 cut(s) 304, 661, 1953
ArsI GACNNNNNNTTYG 2 cut(s) 141, 173
Asp700I GAANNNNTTC 2 cut(s) 473, 1711
AspLEI GCGC 3 cut(s) 641, 729, 1228
AspS9I GGNCC 3 cut(s) 965, 1392, 1780
AsuC2I CCSGG 1 cut(s) 1396
AsuHPI GGTGA 3 cut(s) 730, 782, 1641
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 1 cut(s) 1780
BamHI GGATCC 1 cut(s) 1075
BanI GGYRCC 1 cut(s) 726
BanII GRGCYC 1 cut(s) 216
BbsI GAAGAC 1 cut(s) 1704
Bbv12I GWGCWC 2 cut(s) 216, 867
BbvI GCAGC 4 cut(s) 48, 87, 211, 954
BccI CCATC 3 cut(s) 296, 549, 1654
BceAI ACGGC 2 cut(s) 1496, 1893
BciT130I CCWGG 4 cut(s) 269, 716, 1630, 1902
BciVI GTATCC 2 cut(s) 256, 1720
BcnI CCSGG 1 cut(s) 1396
BcoDI GTCTC 1 cut(s) 927
BfaI CTAG 2 cut(s) 684, 1562
BfmI CTRYAG 3 cut(s) 457, 940, 1785
BfoI RGCGCY 1 cut(s) 730
BfuI GTATCC 2 cut(s) 256, 1720
BglII AGATCT 1 cut(s) 625
BisI GCNGC 7 cut(s) 37, 86, 101, 200, 725, 943, 1406
BlpI GCTNAGC 1 cut(s) 118
BlsI GCNGC 7 cut(s) 38, 87, 102, 201, 726, 944, 1407
BmcAI AGTACT 1 cut(s) 1264
Bme1390I CCNGG 5 cut(s) 269, 716, 1396, 1630, 1902
Bme18I GGWCC 1 cut(s) 1780
BmeT110I CYCGRG 1 cut(s) 194
BmgT120I GGNCC 3 cut(s) 965, 1392, 1780
BmiI GGNNCC 5 cut(s) 728, 967, 1077, 1529, 1782
BmrFI CCNGG 5 cut(s) 269, 716, 1396, 1630, 1902
BmsI GCATC 3 cut(s) 223, 1002, 1116
BpiI GAAGAC 1 cut(s) 1704
BpmI CTGGAG 1 cut(s) 402
Bpu10I CCTNAGC 1 cut(s) 282
Bpu1102I GCTNAGC 1 cut(s) 118
BpuEI CTTGAG 2 cut(s) 495, 769
BpuMI CCSGG 1 cut(s) 1396
BsaBI GATNNNNATC 3 cut(s) 359, 1074, 1356
BsaHI GRCGYC 1 cut(s) 727
BsaI GGTCTC 1 cut(s) 927
BsaJI CCNNGG 6 cut(s) 267, 730, 1063, 1134, 1395, 1629
Bsc4I CCNNNNNNNGG 6 cut(s) 273, 1348, 1401, 1479, 1503, 1631
Bse3DI GCAATG 1 cut(s) 375
Bse8I GATNNNNATC 3 cut(s) 359, 1074, 1356
BseBI CCWGG 4 cut(s) 269, 716, 1630, 1902
BseDI CCNNGG 6 cut(s) 267, 730, 1063, 1134, 1395, 1629
BseGI GGATG 6 cut(s) 34, 69, 327, 412, 1493, 1885
BseJI GATNNNNATC 3 cut(s) 359, 1074, 1356
BseLI CCNNNNNNNGG 6 cut(s) 273, 1348, 1401, 1479, 1503, 1631
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 5 cut(s) 132, 197, 1257, 1418, 1604
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 4 cut(s) 48, 87, 211, 954
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 5 cut(s) 287, 966, 1394, 1405, 1483
BshNI GGYRCC 1 cut(s) 726
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 2 cut(s) 216, 867
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 1 cut(s) 1395
BslFI GGGAC 3 cut(s) 1325, 1382, 1766
BslI CCNNNNNNNGG 6 cut(s) 273, 1348, 1401, 1479, 1503, 1631
BsmAI GTCTC 1 cut(s) 927
BsmFI GGGAC 3 cut(s) 1325, 1382, 1766
BsnI GGCC 5 cut(s) 287, 966, 1394, 1405, 1483
Bso31I GGTCTC 1 cut(s) 927
BsoBI CYCGRG 1 cut(s) 194
Bsp1286I GDGCHC 2 cut(s) 216, 867
Bsp1720I GCTNAGC 1 cut(s) 118
BspACI CCGC 5 cut(s) 85, 137, 724, 1406, 1568
BspANI GGCC 5 cut(s) 287, 966, 1394, 1405, 1483
BspCNI CTCAG 5 cut(s) 131, 198, 1258, 1419, 1605
BspLI GGNNCC 5 cut(s) 728, 967, 1077, 1529, 1782
BspMAI CTGCAG 2 cut(s) 944, 1789
BspPI GGATC 7 cut(s) 408, 655, 709, 1070, 1083, 1697, 1787
BspQI GCTCTTC 2 cut(s) 872, 1236
BspT107I GGYRCC 1 cut(s) 726
BspTNI GGTCTC 1 cut(s) 927
BsrDI GCAATG 1 cut(s) 375
BssECI CCNNGG 6 cut(s) 267, 730, 1063, 1134, 1395, 1629
BssNI GRCGYC 1 cut(s) 727
BssT1I CCWWGG 2 cut(s) 730, 1134
Bst2UI CCWGG 4 cut(s) 269, 716, 1630, 1902
Bst4CI ACNGT 2 cut(s) 1527, 1592
Bst6I CTCTTC 5 cut(s) 567, 872, 996, 1236, 1814
BstACI GRCGYC 1 cut(s) 727
BstC8I GCNNGC 5 cut(s) 346, 350, 1131, 1460, 1559
BstDEI CTNAG 7 cut(s) 118, 206, 282, 705, 1266, 1427, 1613
BstDSI CCRYGG 1 cut(s) 1063
BstF5I GGATG 6 cut(s) 34, 69, 327, 412, 1493, 1885
BstH2I RGCGCY 1 cut(s) 730
BstHHI GCGC 3 cut(s) 641, 729, 1228
BstMAI GTCTC 1 cut(s) 927
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 1 cut(s) 1567
BstNI CCWGG 4 cut(s) 269, 716, 1630, 1902
BstSCI CCNGG 5 cut(s) 267, 714, 1394, 1628, 1900
BstSFI CTRYAG 3 cut(s) 457, 940, 1785
BstV1I GCAGC 4 cut(s) 48, 87, 211, 954
BstV2I GAAGAC 1 cut(s) 1704
BstX2I RGATCY 3 cut(s) 625, 701, 1075
BstYI RGATCY 3 cut(s) 625, 701, 1075
BsuI GTATCC 2 cut(s) 256, 1720
BsuRI GGCC 5 cut(s) 287, 966, 1394, 1405, 1483
BtgI CCRYGG 1 cut(s) 1063
BtgZI GCGATG 2 cut(s) 1550, 1782
BtsCI GGATG 6 cut(s) 34, 69, 327, 412, 1493, 1885
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 1 cut(s) 95
Cac8I GCNNGC 5 cut(s) 346, 350, 1131, 1460, 1559
CaiI CAGNNNCTG 2 cut(s) 205, 1739
CfoI GCGC 3 cut(s) 641, 729, 1228
Cfr13I GGNCC 3 cut(s) 965, 1392, 1780
CsiI ACCWGGT 2 cut(s) 714, 1900
Csp6I GTAC 4 cut(s) 445, 1088, 1263, 1276
CviAII CATG 3 cut(s) 178, 430, 1929
CviQI GTAC 4 cut(s) 445, 1088, 1263, 1276
DdeI CTNAG 7 cut(s) 118, 206, 282, 705, 1266, 1427, 1613
DinI GGCGCC 1 cut(s) 728
EaeI YGGCCR 1 cut(s) 1481
Eam1104I CTCTTC 5 cut(s) 567, 872, 996, 1236, 1814
EarI CTCTTC 5 cut(s) 567, 872, 996, 1236, 1814
Ecl136II GAGCTC 1 cut(s) 214
Eco130I CCWWGG 2 cut(s) 730, 1134
Eco24I GRGCYC 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 927
Eco32I GATATC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 1780
Eco53kI GAGCTC 1 cut(s) 214
Eco57I CTGAAG 4 cut(s) 97, 1251, 1716, 1742
Eco88I CYCGRG 1 cut(s) 194
EcoICRI GAGCTC 1 cut(s) 214
EcoRI GAATTC 1 cut(s) 304
EcoRII CCWGG 4 cut(s) 267, 714, 1628, 1900
EcoRV GATATC 1 cut(s) 109
EcoT14I CCWWGG 2 cut(s) 730, 1134
EcoT38I GRGCYC 1 cut(s) 216
EgeI GGCGCC 1 cut(s) 728
EheI GGCGCC 1 cut(s) 728
ErhI CCWWGG 2 cut(s) 730, 1134
FaeI CATG 3 cut(s) 181, 433, 1932
FalI AAGNNNNNCTT 4 cut(s) 1195, 1227, 1696, 1728
FaqI GGGAC 3 cut(s) 1325, 1382, 1766
FatI CATG 3 cut(s) 177, 429, 1928
FauI CCCGC 1 cut(s) 1575
FauNDI CATATG 1 cut(s) 124
FblI GTMKAC 1 cut(s) 979
Fnu4HI GCNGC 7 cut(s) 37, 86, 101, 200, 725, 943, 1406
FokI GGATG 6 cut(s) 41, 56, 314, 419, 1480, 1892
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 7 cut(s) 37, 86, 101, 200, 725, 943, 1406
FspBI CTAG 2 cut(s) 684, 1562
GlaI GCGC 3 cut(s) 640, 728, 1227
GluI GCNGC 7 cut(s) 37, 86, 101, 200, 725, 943, 1406
GsuI CTGGAG 1 cut(s) 402
HaeII RGCGCY 1 cut(s) 730
HaeIII GGCC 5 cut(s) 287, 966, 1394, 1405, 1483
HapII CCGG 1 cut(s) 1395
HhaI GCGC 3 cut(s) 641, 729, 1228
Hin1I GRCGYC 1 cut(s) 727
Hin1II CATG 3 cut(s) 181, 433, 1932
Hin6I GCGC 3 cut(s) 639, 727, 1226
HinP1I GCGC 3 cut(s) 639, 727, 1226
HpaII CCGG 1 cut(s) 1395
HphI GGTGA 3 cut(s) 730, 782, 1641
Hpy166II GTNNAC 1 cut(s) 980
Hpy188III TCNNGA 9 cut(s) 194, 404, 512, 786, 1238, 1327, 1544, 1740, 1864
Hpy8I GTNNAC 1 cut(s) 980
Hpy99I CGWCG 1 cut(s) 1879
HpyAV CCTTC 6 cut(s) 9, 1089, 1112, 1197, 1635, 1717
HpyCH4III ACNGT 2 cut(s) 1527, 1592
HpyCH4IV ACGT 2 cut(s) 976, 1709
HpyCH4V TGCA 7 cut(s) 236, 522, 568, 942, 1025, 1129, 1787
HpyF10VI GCNNNNNNNGC 1 cut(s) 1567
HpyF3I CTNAG 7 cut(s) 118, 206, 282, 705, 1266, 1427, 1613
HpySE526I ACGT 2 cut(s) 976, 1709
Hsp92I GRCGYC 1 cut(s) 727
Hsp92II CATG 3 cut(s) 181, 433, 1932
HspAI GCGC 3 cut(s) 639, 727, 1226
KasI GGCGCC 1 cut(s) 726
LguI GCTCTTC 2 cut(s) 872, 1236
LmnI GCTCC 2 cut(s) 219, 1751
Lsp1109I GCAGC 4 cut(s) 48, 87, 211, 954
LweI GCATC 3 cut(s) 223, 1002, 1116
MabI ACCWGGT 2 cut(s) 714, 1900
MaeI CTAG 2 cut(s) 684, 1562
MaeII ACGT 2 cut(s) 976, 1709
MaeIII GTNAC 5 cut(s) 654, 1121, 1435, 1466, 1745
MflI RGATCY 3 cut(s) 625, 701, 1075
MhlI GDGCHC 2 cut(s) 216, 867
MluCI AATT 7 cut(s) 8, 304, 661, 804, 1020, 1773, 1953
Mly113I GGCGCC 1 cut(s) 727
MlyI GAGTC 4 cut(s) 553, 612, 662, 1610
MmeI TCCRAC 5 cut(s) 378, 801, 1197, 1857, 1914
MroXI GAANNNNTTC 2 cut(s) 473, 1711
MseI TTAA 5 cut(s) 41, 50, 1095, 1521, 1957
MspA1I CMGCKG 2 cut(s) 85, 1408
MspI CCGG 1 cut(s) 1395
MspR9I CCNGG 5 cut(s) 269, 716, 1396, 1630, 1902
MvaI CCWGG 4 cut(s) 269, 716, 1630, 1902
MwoI GCNNNNNNNGC 1 cut(s) 1567
NarI GGCGCC 1 cut(s) 727
NciI CCSGG 1 cut(s) 1396
NdeI CATATG 1 cut(s) 124
NlaIII CATG 3 cut(s) 181, 433, 1932
NlaIV GGNNCC 5 cut(s) 728, 967, 1077, 1529, 1782
NmuCI GTSAC 4 cut(s) 654, 1435, 1466, 1745
PaeR7I CTCGAG 1 cut(s) 194
PciSI GCTCTTC 2 cut(s) 872, 1236
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PdmI GAANNNNTTC 2 cut(s) 473, 1711
PfeI GAWTC 6 cut(s) 128, 533, 1507, 1540, 1621, 1736
PkrI GCNGC 7 cut(s) 38, 87, 102, 201, 726, 944, 1407
Ple19I CGATCG 1 cut(s) 357
PleI GAGTC 4 cut(s) 553, 612, 661, 1609
PluTI GGCGCC 1 cut(s) 730
PpsI GAGTC 4 cut(s) 553, 612, 661, 1609
Psp124BI GAGCTC 1 cut(s) 216
Psp6I CCWGG 4 cut(s) 267, 714, 1628, 1900
PspGI CCWGG 4 cut(s) 267, 714, 1628, 1900
PspN4I GGNNCC 5 cut(s) 728, 967, 1077, 1529, 1782
PspPI GGNCC 3 cut(s) 965, 1392, 1780
PstI CTGCAG 2 cut(s) 944, 1789
PstNI CAGNNNCTG 2 cut(s) 205, 1739
PsuI RGATCY 3 cut(s) 625, 701, 1075
PvuI CGATCG 1 cut(s) 357
RsaI GTAC 4 cut(s) 446, 1089, 1264, 1277
RsaNI GTAC 4 cut(s) 445, 1088, 1263, 1276
SacI GAGCTC 1 cut(s) 216
SapI GCTCTTC 2 cut(s) 872, 1236
SaqAI TTAA 5 cut(s) 41, 50, 1095, 1521, 1957
SatI GCNGC 7 cut(s) 37, 86, 101, 200, 725, 943, 1406
Sau96I GGNCC 3 cut(s) 965, 1392, 1780
ScaI AGTACT 1 cut(s) 1264
SchI GAGTC 4 cut(s) 553, 612, 662, 1610
ScrFI CCNGG 5 cut(s) 269, 716, 1396, 1630, 1902
SduI GDGCHC 2 cut(s) 216, 867
SexAI ACCWGGT 2 cut(s) 714, 1900
SfaNI GCATC 3 cut(s) 223, 1002, 1116
SfcI CTRYAG 3 cut(s) 457, 940, 1785
SfoI GGCGCC 1 cut(s) 728
Sfr274I CTCGAG 1 cut(s) 194
SinI GGWCC 1 cut(s) 1780
SlaI CTCGAG 1 cut(s) 194
SmlI CTYRAG 3 cut(s) 194, 510, 784
SmoI CTYRAG 3 cut(s) 194, 510, 784
Sse9I AATT 7 cut(s) 8, 304, 661, 804, 1020, 1773, 1953
SsiI CCGC 5 cut(s) 85, 137, 724, 1406, 1568
SspDI GGCGCC 1 cut(s) 726
SspMI CTAG 2 cut(s) 684, 1562
SstI GAGCTC 1 cut(s) 216
StyD4I CCNGG 5 cut(s) 267, 714, 1394, 1628, 1900
StyI CCWWGG 2 cut(s) 730, 1134
TaaI ACNGT 2 cut(s) 1527, 1592
TaiI ACGT 2 cut(s) 979, 1712
TaqI TCGA 6 cut(s) 105, 195, 250, 318, 363, 372
TasI AATT 7 cut(s) 8, 304, 661, 804, 1020, 1773, 1953
TatI WGTACW 3 cut(s) 444, 1087, 1262
TauI GCSGC 3 cut(s) 88, 727, 1408
TfiI GAWTC 6 cut(s) 128, 533, 1507, 1540, 1621, 1736
Tru1I TTAA 5 cut(s) 41, 50, 1095, 1521, 1957
Tru9I TTAA 5 cut(s) 41, 50, 1095, 1521, 1957
TscAI CASTG 1 cut(s) 95
TseFI GTSAC 4 cut(s) 654, 1435, 1466, 1745
TseI GCWGC 4 cut(s) 36, 100, 199, 942
Tsp45I GTSAC 4 cut(s) 654, 1435, 1466, 1745
TspDTI ATGAA 9 cut(s) 141, 194, 423, 561, 903, 1204, 1553, 1785, 1917
TspGWI ACGGA 4 cut(s) 660, 1080, 1764, 1785
TspRI CASTG 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 1780
XapI RAATTY 3 cut(s) 304, 661, 1953
XhoI CTCGAG 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 979
XmnI GAANNNNTTC 2 cut(s) 473, 1711
XspI CTAG 2 cut(s) 684, 1562
ZrmI AGTACT 1 cut(s) 1264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.