Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011225

Physical Location & Seq

Genomic Coordinates Forward (+)

739544 .. 744184

Gene Structure 4641 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G03090.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2145 bp

ATGTCGATGATGACGGTGTGGGCACTTCGACGGAATGTTCGTCGGAAAAATCACTCGATGCTCGTGAGATATATTTCCGGCTCCGCATCGATGAAGCCTAAGGAGCAATGTATTGAGAAGATTTTGGTGGCGAACAGAGGCGAGATTGCTTGCCGGATCATGAGAACGGCGAAGCGTTTAGGGATTCAGACCGTTGCGGTTTACAGTGACGCTGATAGAGATTCGCTCCATGTGAAATCTGCAGACGAAGCGGTACGGATTGGCCCGCCCTCGGCTAGACTTAGTTACCTCAGCGGTGTGACTATCATGGAGGCAGCGGCTCGGACTGGTGCACAGGCAATACACCCTGGCTATGGGTTCTTGTCAGAAAGTTCTGACTTTGCCCAACTCTGTGAGGATTCGGGTCTAACATTCATTGGACCTCCAGCATCTGCTATTAGAGACATGGGTGATAAAAGTGCATCCAAGAGAATTATGGGAGCTGCTGGAGTTCCTCTCGTGCCTGGATATCATGGCCATGAGCAAGATATTGATCATATGAAGTCAGAAGCTGAAAAGATTGGATACCCTATAATAATCAAGCCAACACATGGAGGTGGGGGGAAGGGAATGAGGATAGTGCAAAGTGGGAAGGATTTTGCTGACTCGTTTTTGGGTGCCCAACGTGAAGCTGCTGCTTCTTTTGGCGTTAACACTATTCTTCTCGAGAAATACATTACTCGACCGAGGCATATAGAAGTCCAGATATTTGGTGACAAACATGGAAATGTTCTTCATCTATACGAAAGGGATTGCAGTGTGCAAAGAAGACACCAAAAGATTATTGAAGAAGCTCCTGCGGCAGTTGGATATTATAATGCTGGGACGGTGGAATTTATAGTTGACACCGAGTCTGATCAATTTTATTTTATGGAAATGAACACCCGTCTACAGGTCGAACATCCTGTAACTGAGATGATTGTTGGTCAAGATCTTGTGGAATGGCAAATTCGGGTTGCCAATGGGGAACCTCTCCCCTTAAGCCAATCCGAGGTGCCAATGTCAGGTCATGCCTTTGAGGCCAGGATATATGCTGAAAACGTTCCAAAAGGATTTCTTCCTGCAACTGGGGTCCTCAATCATTATCGCCCTGTTGCAGTCTCACCATCAGTTCGGGTTGAAACTGGAGTTGAGCAAGGAGACACTGTTAGCATGCACTATGATCCTATGATTGCAAAGCTTGTTGTCTGGGGAGGTAATCGTGGCGAAGCTTTAGTGAAACTGAAGGATTGCTTGTCTAACTTTCAGGTAGCAGGTGTACCTACGAACATAAATTTCCTTCAAAAACTTGCTAGTCATAAGGAGTTTGCAGTGGGCAATGTAGAAACTCATTTTATTGAGCACCATAAAAGTGATCTATTTGCTGACGAAAGCAATCCAGCTGCAACAGAAGTGGCATACAAGGCAGTCAAGCATAGTGCAGCATTGGTGGCTGCTTGTATCTCCACAATCGAGCATTCTACTTGGAATGAAAGTAATCATGGGAAAGTTCCATCGATATGGTATTCGAATCCTCCTTTTAGGGTCCATCATGAAGCCAAACAAACCATTGAGCTAGAATGGAATAATGAATGCGAGGGAACTGGCTCTAACCTCATATCACTCGGTGTAAGATATCAACCAGATGGAAGCTATCTCATTGAGGAAGGCAATGATTCTCCAAGTTTAGAACTCAGAGTAACACGAGCAGGAAAGTGCGATTTTAGAGTTGAAGCGGCCGGATTGAGCATGAATGTTAGTCTAGCTGCGTACTTGAAGGATGGTTATAAGCATATCCATATATGGCATGGTTCAGAACACCATCAGTTCAAGCAGAAGGTAGGAATCGAATTCTCTGAAGATGAAGAAGGTGTCCAACACAGAACCAGCTCCGAAACATCATCACACCCTCCAGGAACCATTGTGGCCCCCATGGCTGGTTTGGTAGTCAAGGTCCTTGTCGAAAACGAAGCCAAAGTAGATCAAGGTCAACCTATATTAGTCCTAGAGGCAATGAAGATGGAGCACGTTGTGAAAGCACCATCCTCTGGAAGCATACAGGACCTCAAAGTTAAAGCAGGCCAACAGGTTTCAGATGGCAGTGCTCTATTCAGGATCAAAGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004485 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0005840 GO:0006082 GO:0006520 GO:0006551 GO:0006552 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009507 GO:0009536 GO:0009987 GO:0016054 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022626 GO:0031974 GO:0032991 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046395 GO:0046872 GO:0046914 GO:0050897 GO:0070013 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00280 ko01100 map00280 map01100

Pfam Domains

Protein Families

Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2

Protein Analysis

714

Amino Acids

78.37

Weight (kDa)

6.31

Isoelectric Point (pI)

40.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_carb_N	PF00289	38 - 146	3.1e-44	Biotin carboxylase, N-terminal domain
CPSase_L_D2	PF02786	151 - 359	2.2e-76	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	168 - 329	2.3e-07	ATP-grasp domain
Dala_Dala_lig_C	PF07478	175 - 328	8.4e-11	D-ala D-ala ligase C-terminus
Biotin_carb_C	PF02785	373 - 480	3e-35	Biotin carboxylase C-terminal domain
MCCA_BT	PF19331	494 - 652	1.2e-68	Methylcrotonoyl-CoA carboxylase subunit alpha BT domain
Biotin_lipoyl	PF00364	666 - 732	1.2e-16	Biotin-requiring enzyme
BSH_CusB	PF25919	667 - 733	1.2e-07	CusB, barrel-sandwich hybrid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0011225)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03090 AT1G03090
fragaria_vesca	FvH4_2g23110 FvH4_2g23110 FvH4_2g23110 FvH4_2g23110
malus_domestica	MD11G1280500.v1.1
prunus_persica	Prupe.8G236400_v2.0.a1
pyrus_communis	pycom11g24820
rosa_chinensis	RchiOBHm_Chr6g0290331
rosa_laevigata	RLG00000012226
rosa_multiflora	Rmu_sc0013786.1_g000003
rosa_roxburghii	Rroxscaffold_7G00177150
rosa_rugosa	Rorug06G0211900
rosa_samantha	Rh6AG322200 Rh6BG329500 Rh6CG335400 Rh6DG322400
rosa_wichuraiana	Rw6G027820 Rw6G027920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	855, 1806
AarI	CACCTGC	1 cut(s)	1283
Acc36I	ACCTGC	1 cut(s)	1283
AccB1I	GGYRCC	2 cut(s)	656, 1033
AccB7I	CCANNNNNTGG	2 cut(s)	590, 2066
AccI	GTMKAC	1 cut(s)	928
AciI	CCGC	8 cut(s)	84, 197, 251, 266, 294, 317, 839, 1754
AclI	AACGTT	1 cut(s)	1080
AclWI	GGATC	3 cut(s)	164, 1196, 2141
AcoI	YGGCCR	2 cut(s)	514, 1755
AcsI	RAATTY	4 cut(s)	872, 987, 1312, 1868
AcuI	CTGAAG	2 cut(s)	1283, 1896
AdeI	CACNNNGTG	2 cut(s)	1646, 2050
AfaI	GTAC	3 cut(s)	255, 1299, 1790
AfiI	CCNNNNNNNGG	9 cut(s)	271, 353, 590, 931, 1030, 1043, 1106, 1955, 2066
AflII	CTTAAG	1 cut(s)	1018
AgsI	TTSAA	6 cut(s)	827, 1160, 1322, 1751, 1795, 1849
AhdI	GACNNNNNGTC	1 cut(s)	889
AjnI	CCWGG	4 cut(s)	346, 502, 1061, 1930
Alw21I	GWGCWC	4 cut(s)	334, 1383, 2046, 2125
Alw26I	GTCTC	3 cut(s)	435, 1144, 1173
Alw44I	GTGCAC	1 cut(s)	330
AlwI	GGATC	3 cut(s)	164, 1196, 2141
AlwNI	CAGNNNCTG	2 cut(s)	431, 551
Ama87I	CYCGRG	1 cut(s)	704
AoxI	GGCC	6 cut(s)	262, 514, 1059, 1755, 1944, 2098
ApaLI	GTGCAC	1 cut(s)	330
ApeKI	GCWGC	8 cut(s)	314, 482, 671, 674, 1421, 1460, 1472, 1784
ApoI	RAATTY	4 cut(s)	872, 987, 1312, 1868
ArsI	GACNNNNNNTTYG	2 cut(s)	21, 53
Asp700I	GAANNNNTTC	1 cut(s)	1080
AspS9I	GGNCC	7 cut(s)	263, 419, 1111, 1564, 1945, 1972, 2080
AsuHPI	GGTGA	3 cut(s)	461, 764, 1134
AsuII	TTCGAA	1 cut(s)	1547
AvaI	CYCGRG	1 cut(s)	704
AvaII	GGWCC	5 cut(s)	419, 1111, 1564, 1972, 2080
AxyI	CCTNAGG	1 cut(s)	99
BaeGI	GKGCMC	3 cut(s)	25, 334, 661
BalI	TGGCCA	1 cut(s)	516
BanI	GGYRCC	2 cut(s)	656, 1033
BauI	CACGAG	3 cut(s)	62, 497, 1722
BbsI	GAAGAC	1 cut(s)	814
Bbv12I	GWGCWC	4 cut(s)	334, 1383, 2046, 2125
BbvCI	CCTCAGC	1 cut(s)	290
BbvI	GCAGC	8 cut(s)	326, 469, 658, 661, 1408, 1459, 1472, 1771
BccI	CCATC	9 cut(s)	1153, 1540, 1575, 1658, 1793, 1848, 2032, 2068, 2108
BceAI	ACGGC	1 cut(s)	183
BciT130I	CCWGG	4 cut(s)	348, 504, 1063, 1932
BciVI	GTATCC	1 cut(s)	557
BclI	TGATCA	2 cut(s)	532, 895
BcoDI	GTCTC	3 cut(s)	435, 1144, 1173
BfaI	CTAG	5 cut(s)	276, 1332, 1595, 1781, 2024
BfmI	CTRYAG	2 cut(s)	240, 929
BfrI	CTTAAG	1 cut(s)	1018
BfuAI	ACCTGC	1 cut(s)	1283
BfuI	GTATCC	1 cut(s)	557
BglI	GCCNNNNNGGC	2 cut(s)	1058, 1952
BglII	AGATCT	1 cut(s)	970
Bme1390I	CCNGG	4 cut(s)	348, 504, 1063, 1932
Bme18I	GGWCC	5 cut(s)	419, 1111, 1564, 1972, 2080
BmeRI	GACNNNNNGTC	1 cut(s)	889
BmeT110I	CYCGRG	1 cut(s)	704
BmgT120I	GGNCC	7 cut(s)	263, 419, 1111, 1564, 1945, 1972, 2080
BmiI	GGNNCC	8 cut(s)	82, 658, 1008, 1035, 1112, 1565, 1936, 1947
BmrFI	CCNGG	4 cut(s)	348, 504, 1063, 1932
BmrI	ACTGGG	1 cut(s)	1116
BmsI	GCATC	4 cut(s)	48, 95, 437, 470
BmuI	ACTGGG	1 cut(s)	1116
BpiI	GAAGAC	1 cut(s)	814
BplI	GAGNNNNNCTC	4 cut(s)	210, 242, 480, 512
BpmI	CTGGAG	4 cut(s)	408, 507, 1185, 1914
Bpu10I	CCTNAGC	1 cut(s)	290
Bpu14I	TTCGAA	1 cut(s)	1547
Bsa29I	ATCGAT	2 cut(s)	89, 1535
BsaBI	GATNNNNATC	1 cut(s)	531
BsaJI	CCNNGG	5 cut(s)	270, 346, 725, 1029, 1950
BsaXI	ACNNNNNCTCC	2 cut(s)	1170, 1200
Bsc4I	CCNNNNNNNGG	9 cut(s)	271, 353, 590, 931, 1030, 1043, 1106, 1955, 2066
Bse1I	ACTGG	4 cut(s)	331, 1111, 1168, 1627
Bse21I	CCTNAGG	1 cut(s)	99
Bse3DI	GCAATG	4 cut(s)	113, 1363, 1696, 2037
Bse8I	GATNNNNATC	1 cut(s)	531
BseBI	CCWGG	4 cut(s)	348, 504, 1063, 1932
BseCI	ATCGAT	2 cut(s)	89, 1535
BseDI	CCNNGG	5 cut(s)	270, 346, 725, 1029, 1950
BseGI	GGATG	4 cut(s)	461, 940, 1804, 2060
BseJI	GATNNNNATC	1 cut(s)	531
BseLI	CCNNNNNNNGG	9 cut(s)	271, 353, 590, 931, 1030, 1043, 1106, 1955, 2066
BseMI	GCAATG	4 cut(s)	113, 1363, 1696, 2037
BseMII	CTCAG	3 cut(s)	304, 942, 1726
BseNI	ACTGG	4 cut(s)	331, 1111, 1168, 1627
BseSI	GKGCMC	3 cut(s)	25, 334, 661
BseX3I	CGGCCG	1 cut(s)	1755
BseXI	GCAGC	8 cut(s)	326, 469, 658, 661, 1408, 1459, 1472, 1771
BseYI	CCCAGC	1 cut(s)	860
BsgI	GTGCAG	1 cut(s)	1479
Bsh1285I	CGRYCG	2 cut(s)	725, 1758
BshFI	GGCC	6 cut(s)	264, 516, 1061, 1757, 1946, 2100
BshNI	GGYRCC	2 cut(s)	656, 1033
BshVI	ATCGAT	2 cut(s)	89, 1535
BsiEI	CGRYCG	2 cut(s)	725, 1758
BsiHKAI	GWGCWC	4 cut(s)	334, 1383, 2046, 2125
BsiHKCI	CYCGRG	1 cut(s)	704
BsiSI	CCGG	3 cut(s)	78, 154, 1758
BslFI	GGGAC	1 cut(s)	877
BslI	CCNNNNNNNGG	9 cut(s)	271, 353, 590, 931, 1030, 1043, 1106, 1955, 2066
BsmAI	GTCTC	3 cut(s)	435, 1144, 1173
BsmFI	GGGAC	1 cut(s)	877
BsmI	GAATGC	2 cut(s)	1495, 1616
BsnI	GGCC	6 cut(s)	264, 516, 1061, 1757, 1946, 2100
BsoBI	CYCGRG	1 cut(s)	704
Bsp119I	TTCGAA	1 cut(s)	1547
Bsp1286I	GDGCHC	6 cut(s)	25, 334, 661, 1383, 2046, 2125
Bsp143I	GATC	8 cut(s)	156, 532, 895, 970, 1201, 1393, 1999, 2133
Bsp19I	CCATGG	1 cut(s)	1950
BspACI	CCGC	8 cut(s)	84, 197, 251, 266, 294, 317, 839, 1754
BspANI	GGCC	6 cut(s)	264, 516, 1061, 1757, 1946, 2100
BspCNI	CTCAG	3 cut(s)	303, 943, 1725
BspDI	ATCGAT	2 cut(s)	89, 1535
BspHI	TCATGA	2 cut(s)	159, 1570
BspLI	GGNNCC	8 cut(s)	82, 658, 1008, 1035, 1112, 1565, 1936, 1947
BspMAI	CTGCAG	1 cut(s)	244
BspMI	ACCTGC	1 cut(s)	1283
BspPI	GGATC	3 cut(s)	164, 1196, 2141
BspT104I	TTCGAA	1 cut(s)	1547
BspT107I	GGYRCC	2 cut(s)	656, 1033
BspTI	CTTAAG	1 cut(s)	1018
BsrDI	GCAATG	4 cut(s)	113, 1363, 1696, 2037
BsrI	ACTGG	4 cut(s)	331, 1111, 1168, 1627
BssECI	CCNNGG	5 cut(s)	270, 346, 725, 1029, 1950
BssMI	GATC	8 cut(s)	156, 532, 895, 970, 1201, 1393, 1999, 2133
BssSI	CACGAG	3 cut(s)	62, 497, 1722
BssT1I	CCWWGG	1 cut(s)	1950
Bst2BI	CACGAG	3 cut(s)	62, 497, 1722
Bst2UI	CCWGG	4 cut(s)	348, 504, 1063, 1932
Bst4CI	ACNGT	5 cut(s)	16, 193, 206, 868, 1186
BstAFI	CTTAAG	1 cut(s)	1018
BstBI	TTCGAA	1 cut(s)	1547
BstC8I	GCNNGC	4 cut(s)	151, 266, 1193, 2098
BstDEI	CTNAG	5 cut(s)	99, 281, 290, 951, 1712
BstDSI	CCRYGG	1 cut(s)	1950
BstF5I	GGATG	4 cut(s)	461, 940, 1804, 2060
BstKTI	GATC	8 cut(s)	159, 535, 898, 973, 1204, 1396, 2002, 2136
BstMAI	GTCTC	3 cut(s)	435, 1144, 1173
BstMBI	GATC	8 cut(s)	156, 532, 895, 970, 1201, 1393, 1999, 2133
BstMCI	CGRYCG	2 cut(s)	725, 1758
BstMWI	GCNNNNNNNGC	7 cut(s)	103, 248, 839, 1058, 1442, 1469, 1952
BstNI	CCWGG	4 cut(s)	348, 504, 1063, 1932
BstNSI	RCATGY	1 cut(s)	1195
BstSCI	CCNGG	4 cut(s)	346, 502, 1061, 1930
BstSFI	CTRYAG	2 cut(s)	240, 929
BstSLI	GKGCMC	3 cut(s)	25, 334, 661
BstV1I	GCAGC	8 cut(s)	326, 469, 658, 661, 1408, 1459, 1472, 1771
BstV2I	GAAGAC	1 cut(s)	814
BstX2I	RGATCY	1 cut(s)	970
BstXI	CCANNNNNNTGG	2 cut(s)	749, 1539
BstYI	RGATCY	1 cut(s)	970
BstZI	CGGCCG	1 cut(s)	1755
Bsu15I	ATCGAT	2 cut(s)	89, 1535
Bsu36I	CCTNAGG	1 cut(s)	99
BsuI	GTATCC	1 cut(s)	557
BsuRI	GGCC	6 cut(s)	264, 516, 1061, 1757, 1946, 2100
BsuTUI	ATCGAT	2 cut(s)	89, 1535
BtgI	CCRYGG	1 cut(s)	1950
BtsCI	GGATG	4 cut(s)	461, 940, 1804, 2060
BtsI	GCAGTG	3 cut(s)	802, 1356, 2125
BtsIMutI	CAGTG	5 cut(s)	211, 802, 1182, 1356, 2125
BveI	ACCTGC	1 cut(s)	1283
Cac8I	GCNNGC	4 cut(s)	151, 266, 1193, 2098
CaiI	CAGNNNCTG	2 cut(s)	431, 551
CciI	TCATGA	2 cut(s)	159, 1570
Cfr13I	GGNCC	7 cut(s)	263, 419, 1111, 1564, 1945, 1972, 2080
ClaI	ATCGAT	2 cut(s)	89, 1535
CseI	GACGC	1 cut(s)	218
Csp6I	GTAC	3 cut(s)	254, 1298, 1789
CspCI	CAANNNNNGTGG	2 cut(s)	1413, 1448
CviQI	GTAC	3 cut(s)	254, 1298, 1789
DdeI	CTNAG	5 cut(s)	99, 281, 290, 951, 1712
DpnI	GATC	8 cut(s)	158, 534, 897, 972, 1203, 1395, 2001, 2135
DpnII	GATC	8 cut(s)	156, 532, 895, 970, 1201, 1393, 1999, 2133
DraIII	CACNNNGTG	2 cut(s)	1646, 2050
DriI	GACNNNNNGTC	1 cut(s)	889
EaeI	YGGCCR	2 cut(s)	514, 1755
EagI	CGGCCG	1 cut(s)	1755
Eam1105I	GACNNNNNGTC	1 cut(s)	889
EclXI	CGGCCG	1 cut(s)	1755
Eco130I	CCWWGG	1 cut(s)	1950
Eco32I	GATATC	2 cut(s)	509, 1655
Eco47I	GGWCC	5 cut(s)	419, 1111, 1564, 1972, 2080
Eco52I	CGGCCG	1 cut(s)	1755
Eco57I	CTGAAG	2 cut(s)	1283, 1896
Eco81I	CCTNAGG	1 cut(s)	99
Eco88I	CYCGRG	1 cut(s)	704
EcoO109I	RGGNCCY	3 cut(s)	1111, 1972, 2080
EcoRI	GAATTC	1 cut(s)	1868
EcoRII	CCWGG	4 cut(s)	346, 502, 1061, 1930
EcoRV	GATATC	2 cut(s)	509, 1655
EcoT14I	CCWWGG	1 cut(s)	1950
ErhI	CCWWGG	1 cut(s)	1950
FalI	AAGNNNNNCTT	4 cut(s)	1080, 1112, 1256, 1288
FaqI	GGGAC	1 cut(s)	877
FauI	CCCGC	1 cut(s)	273
FauNDI	CATATG	1 cut(s)	537
FbaI	TGATCA	2 cut(s)	532, 895
FblI	GTMKAC	1 cut(s)	928
FokI	GGATG	4 cut(s)	448, 927, 1811, 2047
FspBI	CTAG	5 cut(s)	276, 1332, 1595, 1781, 2024
GsaI	CCCAGC	1 cut(s)	864
GsuI	CTGGAG	4 cut(s)	408, 507, 1185, 1914
HaeIII	GGCC	6 cut(s)	264, 516, 1061, 1757, 1946, 2100
HapII	CCGG	3 cut(s)	78, 154, 1758
HgaI	GACGC	1 cut(s)	218
HincII	GTYRAC	3 cut(s)	691, 883, 2009
HindII	GTYRAC	3 cut(s)	691, 883, 2009
HindIII	AAGCTT	2 cut(s)	1217, 1248
HinfI	GANTC	8 cut(s)	184, 221, 398, 644, 890, 1549, 1694, 1863
HpaI	GTTAAC	1 cut(s)	691
HpaII	CCGG	3 cut(s)	78, 154, 1758
HphI	GGTGA	3 cut(s)	461, 764, 1134
Hpy166II	GTNNAC	7 cut(s)	202, 332, 691, 883, 929, 1298, 2009
Hpy188III	TCNNGA	9 cut(s)	64, 160, 704, 706, 742, 968, 1571, 2067, 2131
Hpy8I	GTNNAC	7 cut(s)	202, 332, 691, 883, 929, 1298, 2009
Hpy99I	CGWCG	2 cut(s)	33, 45
HpyAV	CCTTC	8 cut(s)	598, 625, 1258, 1328, 1679, 1789, 1849, 1880
HpyCH4III	ACNGT	5 cut(s)	16, 193, 206, 868, 1186
HpyCH4IV	ACGT	3 cut(s)	664, 1080, 2046
HpyF10VI	GCNNNNNNNGC	7 cut(s)	103, 248, 839, 1058, 1442, 1469, 1952
HpyF3I	CTNAG	5 cut(s)	99, 281, 290, 951, 1712
HpySE526I	ACGT	3 cut(s)	664, 1080, 2046
Ksp22I	TGATCA	2 cut(s)	532, 895
KspAI	GTTAAC	1 cut(s)	691
Kzo9I	GATC	8 cut(s)	156, 532, 895, 970, 1201, 1393, 1999, 2133
LmnI	GCTCC	7 cut(s)	86, 103, 231, 479, 838, 1913, 2041
Lsp1109I	GCAGC	8 cut(s)	326, 469, 658, 661, 1408, 1459, 1472, 1771
LweI	GCATC	4 cut(s)	48, 95, 437, 470
MaeI	CTAG	5 cut(s)	276, 1332, 1595, 1781, 2024
MaeII	ACGT	3 cut(s)	664, 1080, 2046
MaeIII	GTNAC	6 cut(s)	206, 284, 298, 752, 946, 1717
MalI	GATC	8 cut(s)	158, 534, 897, 972, 1203, 1395, 2001, 2135
MboI	GATC	8 cut(s)	156, 532, 895, 970, 1201, 1393, 1999, 2133
MboII	GAAGA	9 cut(s)	130, 692, 764, 819, 839, 1088, 1889, 1895, 2047
MflI	RGATCY	1 cut(s)	970
MhlI	GDGCHC	6 cut(s)	25, 334, 661, 1383, 2046, 2125
MlsI	TGGCCA	1 cut(s)	516
MluCI	AATT	6 cut(s)	471, 872, 899, 987, 1312, 1868
MluNI	TGGCCA	1 cut(s)	516
MlyI	GAGTC	2 cut(s)	638, 899
MmeI	TCCRAC	3 cut(s)	23, 826, 1918
Mox20I	TGGCCA	1 cut(s)	516
MroXI	GAANNNNTTC	1 cut(s)	1080
MscI	TGGCCA	1 cut(s)	516
MseI	TTAA	3 cut(s)	690, 1019, 2091
MslI	CAYNNNNRTG	5 cut(s)	516, 594, 765, 1389, 1537
Msp20I	TGGCCA	1 cut(s)	516
MspA1I	CMGCKG	3 cut(s)	294, 317, 1421
MspCI	CTTAAG	1 cut(s)	1018
MspI	CCGG	3 cut(s)	78, 154, 1758
MspR9I	CCNGG	4 cut(s)	348, 504, 1063, 1932
Mva1269I	GAATGC	2 cut(s)	1495, 1616
MvaI	CCWGG	4 cut(s)	348, 504, 1063, 1932
MwoI	GCNNNNNNNGC	7 cut(s)	103, 248, 839, 1058, 1442, 1469, 1952
NcoI	CCATGG	1 cut(s)	1950
NdeI	CATATG	1 cut(s)	537
NdeII	GATC	8 cut(s)	156, 532, 895, 970, 1201, 1393, 1999, 2133
NlaIV	GGNNCC	8 cut(s)	82, 658, 1008, 1035, 1112, 1565, 1936, 1947
NmeAIII	GCCGAG	1 cut(s)	251
NmuCI	GTSAC	3 cut(s)	206, 298, 752
NspI	RCATGY	1 cut(s)	1195
NspV	TTCGAA	1 cut(s)	1547
PaeI	GCATGC	1 cut(s)	1195
PaeR7I	CTCGAG	1 cut(s)	704
PagI	TCATGA	2 cut(s)	159, 1570
PaqCI	CACCTGC	1 cut(s)	1283
PcsI	WCGNNNNNNNCGW	1 cut(s)	37
PctI	GAATGC	2 cut(s)	1495, 1616
PdmI	GAANNNNTTC	1 cut(s)	1080
PfeI	GAWTC	6 cut(s)	184, 221, 398, 1549, 1694, 1863
PflMI	CCANNNNNTGG	2 cut(s)	590, 2066
PfoI	TCCNGGA	1 cut(s)	1930
PleI	GAGTC	2 cut(s)	638, 898
PpsI	GAGTC	2 cut(s)	638, 898
PpuMI	RGGWCCY	3 cut(s)	1111, 1972, 2080
PsiI	TTATAA	2 cut(s)	855, 1806
Psp1406I	AACGTT	1 cut(s)	1080
Psp5II	RGGWCCY	3 cut(s)	1111, 1972, 2080
Psp6I	CCWGG	4 cut(s)	346, 502, 1061, 1930
PspFI	CCCAGC	1 cut(s)	860
PspGI	CCWGG	4 cut(s)	346, 502, 1061, 1930
PspN4I	GGNNCC	8 cut(s)	82, 658, 1008, 1035, 1112, 1565, 1936, 1947
PspPI	GGNCC	7 cut(s)	263, 419, 1111, 1564, 1945, 1972, 2080
PspPPI	RGGWCCY	3 cut(s)	1111, 1972, 2080
PstI	CTGCAG	1 cut(s)	244
PstNI	CAGNNNCTG	2 cut(s)	431, 551
PsuI	RGATCY	1 cut(s)	970
PvuII	CAGCTG	1 cut(s)	1421
RsaI	GTAC	3 cut(s)	255, 1299, 1790
RsaNI	GTAC	3 cut(s)	254, 1298, 1789
RseI	CAYNNNNRTG	5 cut(s)	516, 594, 765, 1389, 1537
SaqAI	TTAA	3 cut(s)	690, 1019, 2091
Sau3AI	GATC	8 cut(s)	156, 532, 895, 970, 1201, 1393, 1999, 2133
Sau96I	GGNCC	7 cut(s)	263, 419, 1111, 1564, 1945, 1972, 2080
SchI	GAGTC	2 cut(s)	638, 899
ScrFI	CCNGG	4 cut(s)	348, 504, 1063, 1932
SduI	GDGCHC	6 cut(s)	25, 334, 661, 1383, 2046, 2125
SfaNI	GCATC	4 cut(s)	48, 95, 437, 470
SfcI	CTRYAG	2 cut(s)	240, 929
Sfr274I	CTCGAG	1 cut(s)	704
SfuI	TTCGAA	1 cut(s)	1547
SinI	GGWCC	5 cut(s)	419, 1111, 1564, 1972, 2080
SlaI	CTCGAG	1 cut(s)	704
SmiMI	CAYNNNNRTG	5 cut(s)	516, 594, 765, 1389, 1537
SmlI	CTYRAG	2 cut(s)	704, 1018
SmoI	CTYRAG	2 cut(s)	704, 1018
SphI	GCATGC	1 cut(s)	1195
Sse9I	AATT	6 cut(s)	471, 872, 899, 987, 1312, 1868
SsiI	CCGC	8 cut(s)	84, 197, 251, 266, 294, 317, 839, 1754
SspMI	CTAG	5 cut(s)	276, 1332, 1595, 1781, 2024
StyD4I	CCNGG	4 cut(s)	346, 502, 1061, 1930
StyI	CCWWGG	1 cut(s)	1950
TaaI	ACNGT	5 cut(s)	16, 193, 206, 868, 1186
TaiI	ACGT	3 cut(s)	667, 1083, 2049
TaqII	GACCGA	1 cut(s)	739
TasI	AATT	6 cut(s)	471, 872, 899, 987, 1312, 1868
TauI	GCSGC	3 cut(s)	320, 842, 1757
TfiI	GAWTC	6 cut(s)	184, 221, 398, 1549, 1694, 1863
Tru1I	TTAA	3 cut(s)	690, 1019, 2091
Tru9I	TTAA	3 cut(s)	690, 1019, 2091
TscAI	CASTG	5 cut(s)	211, 802, 1189, 1356, 2125
TseFI	GTSAC	3 cut(s)	206, 298, 752
TseI	GCWGC	8 cut(s)	314, 482, 671, 674, 1421, 1460, 1472, 1784
Tsp45I	GTSAC	3 cut(s)	206, 298, 752
TspGWI	ACGGA	2 cut(s)	46, 271
TspRI	CASTG	5 cut(s)	211, 802, 1189, 1356, 2125
Van91I	CCANNNNNTGG	2 cut(s)	590, 2066
Vha464I	CTTAAG	1 cut(s)	1018
VneI	GTGCAC	1 cut(s)	330
VpaK11BI	GGWCC	5 cut(s)	419, 1111, 1564, 1972, 2080
XapI	RAATTY	4 cut(s)	872, 987, 1312, 1868
XceI	RCATGY	1 cut(s)	1195
XcmI	CCANNNNNNNNNTGG	3 cut(s)	472, 1823, 1957
XhoI	CTCGAG	1 cut(s)	704
XmiI	GTMKAC	1 cut(s)	928
XmnI	GAANNNNTTC	1 cut(s)	1080
XspI	CTAG	5 cut(s)	276, 1332, 1595, 1781, 2024

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.