Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0011225

Physical Location & Seq

Genomic Coordinates Reverse (-)

16680246 .. 16687325

Gene Structure 7080 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00177150.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2490 bp

ATGGCGTCAGTGGCCACCGTCCTCCGCCGGAAGCTCTCCGGCAAGCCATTCCATTTCCGATTACTACCAGTCCGATCATTCTCCGACTCGCCGCAGAGAATCGAGAAGATTCTGATAGCCAACCGGGGCGAAATTGCGTGCAGGATCATGAGGACCGCAAAGAGACTCGGAATTCAAACCGTCGCCGTTTTCAGCGACGCCGATAGGCACTCGCTCCACGTCAAATCCGCCGACGAGGCCGTTCGTATCGGTCCGGCTCCGGCTCGGCTCAGCTACCTCGACGCCTCGTCGATTCTTGACGCCGCCGTTCGTACTGGTGCGCAGGCTATCCATCCTGGCTATGGTTTTCTGTCAGAGAGTTCTGACTTTGCTCAACTTTGTGAGGATAAGGGTCTTACCTTTATTGGGCCTCCAGCATCTGCCATCAGGGACATGGGTGATAAAAGTGCGTCAAAGAGAATAATGGGTGCAGCAGGGGTACCTCTTGTGCCTGGATATCATGGGAAAGACCAAGATATTGATCTAATGAAGATAGAAGCCGACAAGATTGGATATCCAATCTTAATAAAGCCAACTCATGGAGGTGGGGGAAAGGGTATGAGGATTGTGCAAAGTCCGGATGAGTTTGTTGAAGCCTTCCTTGGAGCACAACGTGAGGCTGCTGCTTCTTTTGGCGTAAACACAATTTTGCTGGAGAAATATATTACACAGCCAAGACACATAGAAGTCCAGCCAAATGTTTCTGATGACTTCCGGTCTCACTTGGGTCAAGCGGCTGTATCTGCTGCCAAGGCAGTTGGCTATCACAGTGCTGGCACAGTGGAATTTATAGTCGATACAGTCTCAGGCCAATTTTATTTCATGGAGATGAATACCCGTCTTCAGGTTGAGCATCCTGTGACTGAGATGATTGTTGGCCAAGATCTTGTAGAGTGGCAAATTCGCGTTGCAAATGGAGAACACCTTCCCATTAGTCAGTCACAGGTCCCCTTGTCAGGTCATGCTTTTGAAGCCCGTATATATGCTGAAAATGTACCGAAAGGATTCCTTCCTGCAACTGGAGTTCTTCATCACTATCAGCATGCTCCAGTTTCACCAACAGTTCGAGTTGAGACTGGAGTTGAACAAGGAGACAGTGTTAGCATGCATTATGATCCTATGATTGCCAAGCTTGTAGTATGGGGAGAAAATCGTGCAGTAGCACTGGTAAAACTGAAGGATTGCTTAACAAAGTTTCAGGTTGCAGGCGTACCAACCAACATCAGTTTTCTGTCAAAACTGGCCAACCATCCGGAATTTGAAAATGGGAATGTGGAAACTCATTTTATTGAGCATTTTAAAGATGACCTGTTTGTCAACCATAGTAATTTGGAAGTAACGAACACAGTGCTTGGTGCTGCTAGATTTGGTGCCAAATTGGCAGCTGCATGTCTCATTGAAAAGGAAAATTTTGTATTCAGAGAAAATCTTCCTGGAAGCAACAACACGATCTCCATATGGTATTCTTCTCTGCCTTTCCGTGTCAATCATTGTGCTAGGCATACAATGGAACTCGAGTGGGAGAATGAATGTGATAGCAGAGGCTCAAAGTTATTGACCTATTCCATCACTTACAAATCAGATGGGAGCTATCTTATTGAGACAGAAGAAGACATTTCCCCACCTTTGGAGGTTAAAGCAACTTGTGGAGGTGATCATGATTTTAGAGTTGAAGCTGATGGTGTAATATTGGATGTTAGCTTAGCTGTTTACTCGAAGGATCAGGCCAAGCACATTCATCTGTGGCATGGGTCACATCATCATCATTTCAGACAAAAATTGGGTCTTGAATTGTCCAATGAAGATGAAACCGAACATAGGCCCAGTTTTGACAGATCATCTCATCCTCCGGGAACAGTTGTTGCACCCATGGCTGGTTTGGTGGTCAAGGTTCTTATTAAGGATGCAACAAAAGTGGAGGAAGGACAACCTATTTTAGTATTAGAGGCAATGAAGATGGAGCATGCTGTAAAAGCACCATCAGCAGGCTATGTCCACGGGCTTCATCTAGCAGCTGGACAGCAGGTTTCTGATGGCAGTGTTCTCTTCAGTATTAAGGTTAGCACTTTACTTTCTAGTACCATTGATGTCAGTCAGTGGTACTGGAATAGGATACACTTACGAACTTGTAATACTGCCGAGGCATTACGCAGTGGCGCCTGGGGCGGTGGTGGTAGTGGGAAGAAGAGGAGGGAAGGGTATGAGGGTTATGGGTACGGTGGTGGGCGGTTCCGGATCGGGCACCAAGTTGAGTGCTTTGGCTTTAGCAATTGCAATTGGGATACGCTTTGTTTTTTGAATCATATGATGGCGGAGGAGTCGCGGGAGTGGCAACCGACAAAGGATTGGGGAGTAGGGAAGGGAGTCGACGGGGTTGAGGATGAGGGTGGACCGAAAAGACGTGGTGGTCTTCTTGCCGTCGTGGGAGTCGCAGGAGGGGCATGGACGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004485 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0005840 GO:0006082 GO:0006520 GO:0006551 GO:0006552 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009507 GO:0009536 GO:0009987 GO:0016054 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022626 GO:0031974 GO:0032991 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046395 GO:0046872 GO:0046914 GO:0050897 GO:0070013 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00280 ko01100 map00280 map01100

Pfam Domains

Protein Families

Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2

Protein Analysis

829

Amino Acids

90.8

Weight (kDa)

6.31

Isoelectric Point (pI)

37.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_carb_N	PF00289	34 - 142	5.7e-45	Biotin carboxylase, N-terminal domain
CPSase_L_D2	PF02786	147 - 244	1.4e-25	Carbamoyl-phosphate synthase L chain, ATP binding domain
CPSase_L_D2	PF02786	247 - 323	1.6e-24	Carbamoyl-phosphate synthase L chain, ATP binding domain
Biotin_carb_C	PF02785	337 - 444	3.7e-36	Biotin carboxylase C-terminal domain
MCCA_BT	PF19331	457 - 620	1.5e-75	Methylcrotonoyl-CoA carboxylase subunit alpha BT domain
Biotin_lipoyl	PF00364	632 - 698	8.4e-16	Biotin-requiring enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011225)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03090 AT1G03090
fragaria_vesca	FvH4_2g23110 FvH4_2g23110 FvH4_2g23110 FvH4_2g23110
malus_domestica	MD11G1280500.v1.1
prunus_persica	Prupe.8G236400_v2.0.a1
pyrus_communis	pycom11g24820
rosa_chinensis	RchiOBHm_Chr6g0290331
rosa_laevigata	RLG00000012226
rosa_multiflora	Rmu_sc0013786.1_g000003
rosa_roxburghii	Rroxscaffold_7G00177150
rosa_rugosa	Rorug06G0211900
rosa_samantha	Rh6AG322200 Rh6BG329500 Rh6CG335400 Rh6DG322400
rosa_wichuraiana	Rw6G027820 Rw6G027920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1352, 2445
Acc16I	TGCGCA	1 cut(s)	321
Acc36I	ACCTGC	1 cut(s)	2053
Acc65I	GGTACC	1 cut(s)	478
AccB1I	GGYRCC	4 cut(s)	478, 1409, 2195, 2280
AccB7I	CCANNNNNTGG	1 cut(s)	578
AccI	GTMKAC	1 cut(s)	2406
AccII	CGCG	2 cut(s)	945, 2362
AccIII	TCCGGA	3 cut(s)	616, 1291, 2271
AclWI	GGATC	4 cut(s)	152, 1148, 1767, 2282
AcoI	YGGCCR	3 cut(s)	12, 916, 1281
AcsI	RAATTY	5 cut(s)	171, 824, 939, 1295, 1447
AcuI	CTGAAG	3 cut(s)	866, 1235, 2071
AcyI	GRCGYC	5 cut(s)	5, 198, 282, 300, 2196
AdeI	CACNNNGTG	1 cut(s)	653
AfaI	GTAC	7 cut(s)	313, 480, 1035, 1251, 2119, 2141, 2255
AgsI	TTSAA	9 cut(s)	176, 632, 1010, 1124, 1301, 1439, 1712, 1829, 2338
AhdI	GACNNNNNGTC	2 cut(s)	286, 754
AjiI	CACGTC	2 cut(s)	220, 2441
AjnI	CCWGG	4 cut(s)	334, 490, 1471, 2198
AjuI	GAANNNNNNNTTGG	2 cut(s)	624, 656
AluBI	AGCT	9 cut(s)	34, 273, 1171, 1424, 1629, 1715, 1740, 1745, 2054
AluI	AGCT	9 cut(s)	34, 273, 1171, 1424, 1629, 1715, 1740, 1745, 2054
Alw21I	GWGCWC	1 cut(s)	649
Alw26I	GTCTC	7 cut(s)	157, 762, 847, 1106, 1125, 1436, 1634
AlwI	GGATC	4 cut(s)	152, 1148, 1767, 2282
AlwNI	CAGNNNCTG	1 cut(s)	419
Ama87I	CYCGRG	1 cut(s)	1553
Aor13HI	TCCGGA	3 cut(s)	616, 1291, 2271
AoxI	GGCC	8 cut(s)	12, 237, 407, 847, 916, 1281, 1764, 1859
ApeKI	GCWGC	8 cut(s)	470, 659, 662, 785, 1397, 1421, 1424, 2051
ApoI	RAATTY	5 cut(s)	171, 824, 939, 1295, 1447
ArsI	GACNNNNNNTTYG	2 cut(s)	1807, 1839
Asp700I	GAANNNNTTC	3 cut(s)	963, 1043, 1467
Asp718I	GGTACC	1 cut(s)	478
AspLEI	GCGC	2 cut(s)	322, 2198
AspS9I	GGNCC	6 cut(s)	153, 251, 407, 985, 1860, 2429
AsuC2I	CCSGG	2 cut(s)	125, 1890
AsuHPI	GGTGA	3 cut(s)	449, 1086, 1703
AvaI	CYCGRG	1 cut(s)	1553
AvaII	GGWCC	4 cut(s)	153, 251, 985, 2429
BaeGI	GKGCMC	1 cut(s)	2283
BaeI	ACNNNNGTAYC	2 cut(s)	2245, 2278
BalI	TGGCCA	3 cut(s)	14, 918, 1283
BanI	GGYRCC	4 cut(s)	478, 1409, 2195, 2280
BbsI	GAAGAC	3 cut(s)	872, 1656, 2441
Bbv12I	GWGCWC	1 cut(s)	649
BbvI	GCAGC	8 cut(s)	482, 646, 649, 772, 1384, 1411, 1433, 2063
BceAI	ACGGC	4 cut(s)	170, 224, 290, 2441
BcgI	CGANNNNNNTGC	2 cut(s)	1369, 1403
BciT130I	CCWGG	4 cut(s)	336, 492, 1473, 2200
BciVI	GTATCC	2 cut(s)	2145, 2314
BclI	TGATCA	1 cut(s)	1693
BcnI	CCSGG	2 cut(s)	125, 1890
BcoDI	GTCTC	7 cut(s)	157, 762, 847, 1106, 1125, 1436, 1634
BfaI	CTAG	4 cut(s)	1401, 1536, 2048, 2115
BfoI	RGCGCY	1 cut(s)	2199
BfuAI	ACCTGC	1 cut(s)	2053
BfuI	GTATCC	2 cut(s)	2145, 2314
BglI	GCCNNNNNGGC	2 cut(s)	236, 1418
BglII	AGATCT	1 cut(s)	922
BlpI	GCTNAGC	2 cut(s)	269, 1741
Bme1390I	CCNGG	6 cut(s)	125, 336, 492, 1473, 1890, 2200
Bme18I	GGWCC	4 cut(s)	153, 251, 985, 2429
BmeRI	GACNNNNNGTC	2 cut(s)	286, 754
BmeT110I	CYCGRG	1 cut(s)	1553
BmgBI	CACGTC	2 cut(s)	220, 2441
BmgT120I	GGNCC	6 cut(s)	153, 251, 407, 985, 1860, 2429
BmiI	GGNNCC	7 cut(s)	258, 480, 987, 1411, 2197, 2270, 2282
BmrFI	CCNGG	6 cut(s)	125, 336, 492, 1473, 1890, 2200
BmrI	ACTGGG	1 cut(s)	1857
BmsI	GCATC	3 cut(s)	425, 901, 1933
BmuI	ACTGGG	1 cut(s)	1857
BpiI	GAAGAC	3 cut(s)	872, 1656, 2441
BpmI	CTGGAG	5 cut(s)	396, 713, 1071, 1080, 1137
Bpu1102I	GCTNAGC	2 cut(s)	269, 1741
BpuMI	CCSGG	2 cut(s)	125, 1890
BsaBI	GATNNNNATC	1 cut(s)	519
BsaHI	GRCGYC	5 cut(s)	5, 198, 282, 300, 2196
BsaI	GGTCTC	1 cut(s)	762
BsaJI	CCNNGG	7 cut(s)	124, 640, 789, 1908, 2035, 2178, 2199
BsaWI	WCCGGW	4 cut(s)	616, 753, 1291, 2271
BsaXI	ACNNNNNCTCC	4 cut(s)	1122, 1152, 1475, 1505
Bse1I	ACTGG	9 cut(s)	68, 319, 1063, 1088, 1120, 1209, 1284, 1863, 2147
Bse3DI	GCAATG	1 cut(s)	1995
Bse8I	GATNNNNATC	1 cut(s)	519
BseAI	TCCGGA	3 cut(s)	616, 1291, 2271
BseBI	CCWGG	4 cut(s)	336, 492, 1473, 2200
BseDI	CCNNGG	7 cut(s)	124, 640, 789, 1908, 2035, 2178, 2199
BseGI	GGATG	8 cut(s)	331, 625, 892, 1288, 1738, 1882, 1948, 2425
BseJI	GATNNNNATC	1 cut(s)	519
BseMI	GCAATG	1 cut(s)	1995
BseMII	CTCAG	3 cut(s)	283, 858, 894
BseNI	ACTGG	9 cut(s)	68, 319, 1063, 1088, 1120, 1209, 1284, 1863, 2147
BseRI	GAGGAG	2 cut(s)	2242, 2369
BseSI	GKGCMC	1 cut(s)	2283
BseXI	GCAGC	8 cut(s)	482, 646, 649, 772, 1384, 1411, 1433, 2063
BsgI	GTGCAG	3 cut(s)	160, 489, 1215
Bsh1236I	CGCG	2 cut(s)	945, 2362
BshFI	GGCC	8 cut(s)	14, 239, 409, 849, 918, 1283, 1766, 1861
BshNI	GGYRCC	4 cut(s)	478, 1409, 2195, 2280
BsiHKAI	GWGCWC	1 cut(s)	649
BsiHKCI	CYCGRG	1 cut(s)	1553
BslFI	GGGAC	2 cut(s)	443, 971
BsmAI	GTCTC	7 cut(s)	157, 762, 847, 1106, 1125, 1436, 1634
BsmFI	GGGAC	2 cut(s)	443, 971
BsnI	GGCC	8 cut(s)	14, 239, 409, 849, 918, 1283, 1766, 1861
Bso31I	GGTCTC	1 cut(s)	762
BsoBI	CYCGRG	1 cut(s)	1553
Bsp1286I	GDGCHC	2 cut(s)	649, 2283
Bsp13I	TCCGGA	3 cut(s)	616, 1291, 2271
Bsp1720I	GCTNAGC	2 cut(s)	269, 1741
Bsp19I	CCATGG	1 cut(s)	1908
BspANI	GGCC	8 cut(s)	14, 239, 409, 849, 918, 1283, 1766, 1861
BspCNI	CTCAG	3 cut(s)	282, 857, 895
BspEI	TCCGGA	3 cut(s)	616, 1291, 2271
BspFNI	CGCG	2 cut(s)	945, 2362
BspHI	TCATGA	2 cut(s)	147, 1696
BspLI	GGNNCC	7 cut(s)	258, 480, 987, 1411, 2197, 2270, 2282
BspMI	ACCTGC	1 cut(s)	2053
BspPI	GGATC	4 cut(s)	152, 1148, 1767, 2282
BspT107I	GGYRCC	4 cut(s)	478, 1409, 2195, 2280
BspTNI	GGTCTC	1 cut(s)	762
BsrDI	GCAATG	1 cut(s)	1995
BsrI	ACTGG	9 cut(s)	68, 319, 1063, 1088, 1120, 1209, 1284, 1863, 2147
BssECI	CCNNGG	7 cut(s)	124, 640, 789, 1908, 2035, 2178, 2199
BssNI	GRCGYC	5 cut(s)	5, 198, 282, 300, 2196
BssT1I	CCWWGG	3 cut(s)	640, 789, 1908
Bst2UI	CCWGG	4 cut(s)	336, 492, 1473, 2200
Bst6I	CTCTTC	2 cut(s)	2090, 2219
BstACI	GRCGYC	5 cut(s)	5, 198, 282, 300, 2196
BstC8I	GCNNGC	9 cut(s)	44, 139, 324, 814, 1083, 1145, 1246, 2004, 2026
BstDEI	CTNAG	4 cut(s)	269, 844, 903, 1741
BstDSI	CCRYGG	2 cut(s)	1908, 2035
BstF5I	GGATG	8 cut(s)	331, 625, 892, 1288, 1738, 1882, 1948, 2425
BstFNI	CGCG	2 cut(s)	945, 2362
BstH2I	RGCGCY	1 cut(s)	2199
BstHHI	GCGC	2 cut(s)	322, 2198
BstMAI	GTCTC	7 cut(s)	157, 762, 847, 1106, 1125, 1436, 1634
BstNI	CCWGG	4 cut(s)	336, 492, 1473, 2200
BstNSI	RCATGY	4 cut(s)	1085, 1147, 1431, 2006
BstSCI	CCNGG	6 cut(s)	123, 334, 490, 1471, 1888, 2198
BstSLI	GKGCMC	1 cut(s)	2283
BstUI	CGCG	2 cut(s)	945, 2362
BstV1I	GCAGC	8 cut(s)	482, 646, 649, 772, 1384, 1411, 1433, 2063
BstV2I	GAAGAC	3 cut(s)	872, 1656, 2441
BstX2I	RGATCY	1 cut(s)	922
BstYI	RGATCY	1 cut(s)	922
BsuI	GTATCC	2 cut(s)	2145, 2314
BsuRI	GGCC	8 cut(s)	14, 239, 409, 849, 918, 1283, 1766, 1861
BtgI	CCRYGG	2 cut(s)	1908, 2035
BtrI	CACGTC	2 cut(s)	220, 2441
BtsCI	GGATG	8 cut(s)	331, 625, 892, 1288, 1738, 1882, 1948, 2425
BtsI	GCAGTG	2 cut(s)	2083, 2197
BtsIMutI	CAGTG	9 cut(s)	15, 814, 825, 1141, 1202, 1392, 2083, 2141, 2197
BveI	ACCTGC	1 cut(s)	2053
Cac8I	GCNNGC	9 cut(s)	44, 139, 324, 814, 1083, 1145, 1246, 2004, 2026
CaiI	CAGNNNCTG	1 cut(s)	419
CciI	TCATGA	2 cut(s)	147, 1696
CfoI	GCGC	2 cut(s)	322, 2198
Cfr13I	GGNCC	6 cut(s)	153, 251, 407, 985, 1860, 2429
CpoI	CGGWCCG	1 cut(s)	251
CseI	GACGC	4 cut(s)	206, 290, 308, 438
Csp6I	GTAC	7 cut(s)	312, 479, 1034, 1250, 2118, 2140, 2254
CspCI	CAANNNNNGTGG	2 cut(s)	1935, 1970
CspI	CGGWCCG	1 cut(s)	251
CviQI	GTAC	7 cut(s)	312, 479, 1034, 1250, 2118, 2140, 2254
DdeI	CTNAG	4 cut(s)	269, 844, 903, 1741
DinI	GGCGCC	1 cut(s)	2197
DraI	TTTAAA	1 cut(s)	1339
DraIII	CACNNNGTG	1 cut(s)	653
DrdI	GACNNNNNNGTC	2 cut(s)	1352, 2445
DriI	GACNNNNNGTC	2 cut(s)	286, 754
DseDI	GACNNNNNNGTC	2 cut(s)	1352, 2445
EaeI	YGGCCR	3 cut(s)	12, 916, 1281
Eam1104I	CTCTTC	2 cut(s)	2090, 2219
Eam1105I	GACNNNNNGTC	2 cut(s)	286, 754
EarI	CTCTTC	2 cut(s)	2090, 2219
EciI	GGCGGA	3 cut(s)	14, 217, 2366
Eco130I	CCWWGG	3 cut(s)	640, 789, 1908
Eco31I	GGTCTC	1 cut(s)	762
Eco32I	GATATC	2 cut(s)	497, 554
Eco47I	GGWCC	4 cut(s)	153, 251, 985, 2429
Eco57I	CTGAAG	3 cut(s)	866, 1235, 2071
Eco88I	CYCGRG	1 cut(s)	1553
EcoO109I	RGGNCCY	1 cut(s)	985
EcoRI	GAATTC	1 cut(s)	171
EcoRII	CCWGG	4 cut(s)	334, 490, 1471, 2198
EcoRV	GATATC	2 cut(s)	497, 554
EcoT14I	CCWWGG	3 cut(s)	640, 789, 1908
EcoT22I	ATGCAT	1 cut(s)	1149
EgeI	GGCGCC	1 cut(s)	2197
EheI	GGCGCC	1 cut(s)	2197
ErhI	CCWWGG	3 cut(s)	640, 789, 1908
FalI	AAGNNNNNCTT	6 cut(s)	624, 656, 1032, 1064, 1208, 1240
FaqI	GGGAC	2 cut(s)	443, 971
FauI	CCCGC	1 cut(s)	2355
FauNDI	CATATG	2 cut(s)	1496, 2343
FbaI	TGATCA	1 cut(s)	1693
FblI	GTMKAC	1 cut(s)	2406
FokI	GGATG	8 cut(s)	318, 632, 879, 1275, 1745, 1869, 1955, 2432
FspBI	CTAG	4 cut(s)	1401, 1536, 2048, 2115
FspI	TGCGCA	1 cut(s)	321
GlaI	GCGC	2 cut(s)	321, 2197
GsuI	CTGGAG	5 cut(s)	396, 713, 1071, 1080, 1137
HaeII	RGCGCY	1 cut(s)	2199
HaeIII	GGCC	8 cut(s)	14, 239, 409, 849, 918, 1283, 1766, 1861
HgaI	GACGC	4 cut(s)	206, 290, 308, 438
HhaI	GCGC	2 cut(s)	322, 2198
Hin1I	GRCGYC	5 cut(s)	5, 198, 282, 300, 2196
Hin6I	GCGC	2 cut(s)	320, 2196
HinP1I	GCGC	2 cut(s)	320, 2196
HincII	GTYRAC	2 cut(s)	1357, 2407
HindII	GTYRAC	2 cut(s)	1357, 2407
HindIII	AAGCTT	1 cut(s)	1169
HphI	GGTGA	3 cut(s)	449, 1086, 1703
Hpy166II	GTNNAC	6 cut(s)	679, 1357, 1750, 2035, 2407, 2429
Hpy188III	TCNNGA	8 cut(s)	103, 148, 296, 617, 1292, 1697, 1826, 2272
Hpy8I	GTNNAC	6 cut(s)	679, 1357, 1750, 2035, 2407, 2429
Hpy99I	CGWCG	7 cut(s)	185, 200, 236, 284, 292, 2411, 2462
HpyAV	CCTTC	8 cut(s)	646, 974, 1058, 1210, 1750, 1955, 2228, 2392
HpyCH4IV	ACGT	4 cut(s)	219, 652, 2440, 2486
HpyF3I	CTNAG	4 cut(s)	269, 844, 903, 1741
HpySE526I	ACGT	4 cut(s)	219, 652, 2440, 2486
Hsp92I	GRCGYC	5 cut(s)	5, 198, 282, 300, 2196
HspAI	GCGC	2 cut(s)	320, 2196
KasI	GGCGCC	1 cut(s)	2195
Kpn2I	TCCGGA	3 cut(s)	616, 1291, 2271
KpnI	GGTACC	1 cut(s)	482
Ksp22I	TGATCA	1 cut(s)	1693
LmnI	GCTCC	6 cut(s)	219, 262, 644, 1090, 1626, 1999
Lsp1109I	GCAGC	8 cut(s)	482, 646, 649, 772, 1384, 1411, 1433, 2063
LweI	GCATC	3 cut(s)	425, 901, 1933
MaeI	CTAG	4 cut(s)	1401, 1536, 2048, 2115
MaeII	ACGT	4 cut(s)	219, 652, 2440, 2486
MaeIII	GTNAC	4 cut(s)	898, 978, 1375, 1791
MfeI	CAATTG	2 cut(s)	2308, 2314
MflI	RGATCY	1 cut(s)	922
MhlI	GDGCHC	2 cut(s)	649, 2283
MlsI	TGGCCA	3 cut(s)	14, 918, 1283
MluNI	TGGCCA	3 cut(s)	14, 918, 1283
Mly113I	GGCGCC	1 cut(s)	2196
MlyI	GAGTC	5 cut(s)	80, 159, 2366, 2412, 2475
MmeI	TCCRAC	1 cut(s)	108
Mox20I	TGGCCA	3 cut(s)	14, 918, 1283
Mph1103I	ATGCAT	1 cut(s)	1149
MroI	TCCGGA	3 cut(s)	616, 1291, 2271
MroXI	GAANNNNTTC	3 cut(s)	963, 1043, 1467
MscI	TGGCCA	3 cut(s)	14, 918, 1283
MseI	TTAA	6 cut(s)	563, 1226, 1338, 1674, 1938, 2094
MslI	CAYNNNNRTG	2 cut(s)	582, 866
Msp20I	TGGCCA	3 cut(s)	14, 918, 1283
MspA1I	CMGCKG	2 cut(s)	1424, 2054
MspR9I	CCNGG	6 cut(s)	125, 336, 492, 1473, 1890, 2200
MunI	CAATTG	2 cut(s)	2308, 2314
MvaI	CCWGG	4 cut(s)	336, 492, 1473, 2200
MvnI	CGCG	2 cut(s)	945, 2362
NarI	GGCGCC	1 cut(s)	2196
NciI	CCSGG	2 cut(s)	125, 1890
NcoI	CCATGG	1 cut(s)	1908
NdeI	CATATG	2 cut(s)	1496, 2343
NlaIV	GGNNCC	7 cut(s)	258, 480, 987, 1411, 2197, 2270, 2282
NmeAIII	GCCGAG	2 cut(s)	244, 2203
NmuCI	GTSAC	3 cut(s)	898, 978, 1791
NsbI	TGCGCA	1 cut(s)	321
NsiI	ATGCAT	1 cut(s)	1149
NspI	RCATGY	4 cut(s)	1085, 1147, 1431, 2006
PaeI	GCATGC	3 cut(s)	1085, 1147, 2006
PaeR7I	CTCGAG	1 cut(s)	1553
PagI	TCATGA	2 cut(s)	147, 1696
PdmI	GAANNNNTTC	3 cut(s)	963, 1043, 1467
PfeI	GAWTC	5 cut(s)	99, 109, 292, 1044, 2338
PflMI	CCANNNNNTGG	1 cut(s)	578
PfoI	TCCNGGA	2 cut(s)	1471, 1888
PleI	GAGTC	5 cut(s)	80, 159, 2365, 2411, 2474
PluTI	GGCGCC	1 cut(s)	2199
PpsI	GAGTC	5 cut(s)	80, 159, 2365, 2411, 2474
PpuMI	RGGWCCY	1 cut(s)	985
Psp5II	RGGWCCY	1 cut(s)	985
Psp6I	CCWGG	4 cut(s)	334, 490, 1471, 2198
PspGI	CCWGG	4 cut(s)	334, 490, 1471, 2198
PspN4I	GGNNCC	7 cut(s)	258, 480, 987, 1411, 2197, 2270, 2282
PspPI	GGNCC	6 cut(s)	153, 251, 407, 985, 1860, 2429
PspPPI	RGGWCCY	1 cut(s)	985
PspXI	VCTCGAGB	1 cut(s)	1553
PsrI	GAACNNNNNNTAC	2 cut(s)	2155, 2187
PstNI	CAGNNNCTG	1 cut(s)	419
PsuI	RGATCY	1 cut(s)	922
PvuII	CAGCTG	2 cut(s)	1424, 2054
RsaI	GTAC	7 cut(s)	313, 480, 1035, 1251, 2119, 2141, 2255
RsaNI	GTAC	7 cut(s)	312, 479, 1034, 1250, 2118, 2140, 2254
RseI	CAYNNNNRTG	2 cut(s)	582, 866
Rsr2I	CGGWCCG	1 cut(s)	251
RsrII	CGGWCCG	1 cut(s)	251
SalI	GTCGAC	1 cut(s)	2405
SaqAI	TTAA	6 cut(s)	563, 1226, 1338, 1674, 1938, 2094
Sau96I	GGNCC	6 cut(s)	153, 251, 407, 985, 1860, 2429
SchI	GAGTC	5 cut(s)	80, 159, 2366, 2412, 2475
ScrFI	CCNGG	6 cut(s)	125, 336, 492, 1473, 1890, 2200
SduI	GDGCHC	2 cut(s)	649, 2283
SfaNI	GCATC	3 cut(s)	425, 901, 1933
SfoI	GGCGCC	1 cut(s)	2197
Sfr274I	CTCGAG	1 cut(s)	1553
SinI	GGWCC	4 cut(s)	153, 251, 985, 2429
SlaI	CTCGAG	1 cut(s)	1553
SmiMI	CAYNNNNRTG	2 cut(s)	582, 866
SmlI	CTYRAG	1 cut(s)	1553
SmoI	CTYRAG	1 cut(s)	1553
SphI	GCATGC	3 cut(s)	1085, 1147, 2006
SspDI	GGCGCC	1 cut(s)	2195
SspI	AATATT	1 cut(s)	1728
SspMI	CTAG	4 cut(s)	1401, 1536, 2048, 2115
StyD4I	CCNGG	6 cut(s)	123, 334, 490, 1471, 1888, 2198
StyI	CCWWGG	3 cut(s)	640, 789, 1908
TaiI	ACGT	4 cut(s)	222, 655, 2443, 2489
TaqI	TCGA	8 cut(s)	102, 279, 290, 834, 1105, 1554, 1754, 2406
TaqII	GACCGA	2 cut(s)	239, 2446
TauI	GCSGC	3 cut(s)	94, 305, 776
TfiI	GAWTC	5 cut(s)	99, 109, 292, 1044, 2338
Tru1I	TTAA	6 cut(s)	563, 1226, 1338, 1674, 1938, 2094
Tru9I	TTAA	6 cut(s)	563, 1226, 1338, 1674, 1938, 2094
TscAI	CASTG	9 cut(s)	15, 814, 825, 1141, 1209, 1392, 2083, 2141, 2197
TseFI	GTSAC	3 cut(s)	898, 978, 1791
TseI	GCWGC	8 cut(s)	470, 659, 662, 785, 1397, 1421, 1424, 2051
Tsp45I	GTSAC	3 cut(s)	898, 978, 1791
TspGWI	ACGGA	1 cut(s)	1508
TspRI	CASTG	9 cut(s)	15, 814, 825, 1141, 1209, 1392, 2083, 2141, 2197
Van91I	CCANNNNNTGG	1 cut(s)	578
VpaK11BI	GGWCC	4 cut(s)	153, 251, 985, 2429
XapI	RAATTY	5 cut(s)	171, 824, 939, 1295, 1447
XceI	RCATGY	4 cut(s)	1085, 1147, 1431, 2006
XcmI	CCANNNNNNNNNTGG	3 cut(s)	338, 430, 1915
XhoI	CTCGAG	1 cut(s)	1553
XmiI	GTMKAC	1 cut(s)	2406
XmnI	GAANNNNTTC	3 cut(s)	963, 1043, 1467
XspI	CTAG	4 cut(s)	1401, 1536, 2048, 2115
Zsp2I	ATGCAT	1 cut(s)	1149

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.