Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0011225

Physical Location & Seq

Genomic Coordinates Forward (+)

51886884 .. 51893030

Gene Structure 6147 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G027920.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2208 bp

ATGGCGTCAGTGGCCACCGTCCTCCGCCGGAAGCTCTCCGGCAAGCCATTCCGTTTCCGATTACTACCACTCCGATCATTCTCCGACTCGCCGCAGAGAATCGAGAAGATTCTGATAGCCAACCGGGGCGAAATTGCGTGCAGGATCATGAGGACCGCAAAGAGACTCGGAATTCAAACCGTCGCCGTTTTCAGCGACGCCGATAGACACTCGCTCCACGTCAAATCCGCCGACGAGGCCGTTCGTATCGGTCCGGCTCCGGCTCGGCTCAGCTACCTCAACGCCTCGTCGATTCTTGACGCCGCCGTTCGTACTGGTGCGCAGGCTATCCATCCTGGCTATGGTTTTCTGTCAGAGAGTTCCGACTTTGCTCAACTTTGTGAGGATAAGGGTCTTACCTTTATTGGGCCTCCAGCATCTGCCATCAGGGACATGGGTGATAAAAGTGCGTCAAAGAGAATAATGGGTGCAGCAGGGGTACCTCTTGTGCCTGGATATCATGGGAAAGACCAAGATATTGATCTAATGAAGATAGAAGCTGACAAGATTGGATATCCAATCTTAATAAAGCCAACTCATGGAGGTGGGGGAAAGGGTATGAGGATTGTGCAAAGTCCGGATGAGTTTGTTGAAGCCTTCCTTGGAGCACAACGTGAGGCTGCTGCTTCTTTTGGCGTAAACACAATTTTGCTGGAGAAATATATTACACAGCCAAGACACATAGAAGTCCAGATATTTGGGGATAAACACGGGAATGTTCTACATTTATATGAGAGAGATTGCAGCGTACAAAGAAGGCACCAGAAAATAATCGAAGAAGCCCCAGCTCCAAATGTTTCTGATGACTTCCGGTCTCACTTGGGTCAAGCGGCTGTATCTGCTGCCAAGGCAGTTAGCTATCACAGTGCCGGCACAGTGGAATTTATAGTCGATACAGTCTCAGGCCAATTTTATTTCATGGAGATGAATACCCGTCTTCAGGTTGAGCATCCTGTGACTGAGATGATTGTTGGCCAAGATCTTGTAGAGTGGCAAATTCGTGTAGCAAATGGAGAACACCTTCCCATTAGTCAGTCACAGGTCCCTTTGTCAGGTCATGCTTTTGAAGCCCGTATATATGCTGAAAATGTACCAAAAGGGTTCCTTCCTGCAACTGGAGTTCTTCATCACTATCAGCATGCTCCAGTTTCACCATCAGTTCGAGTTGAGACTGGAGTTGAACAAGGAGACAGTGTTAGCATGCATTATGATCCTATGATTGCCAAGCTTGTAGTATGGGGAGAAAATCGTGCAGTAGCACTGGTAAAACTGAAGGATTGCTTGACAAAGTTTCAGGTTGCAGGTGTACCAACCAACATCAGTTTTCTGTCAAAACTTGCCAACCATCGGGAATTTGAAAATGGGAATGTGGAAACTCATTTTATTGAACATTTTAAAGATGACCTGTTTGTCAACCCTAGTAATTTGGAAGTAATGAACACAGTGCTTGGTGCTGCTAGATTTGTTGCCAAACTGGCGGCTGCATGTCTCATTGAAAAGGAAAATTCTGTATTCAGAGAAAATCTTCCTGGAAGCAACAACACGATCTCCATATGGTATTCTTCTCCGCCTTTCAGAGTCAATCATTGTGCTAGGCATACAATGGAACTCGAGTGGGAGAATGAATATGATAGCAGTGGCTCAAGGTTATTGACCTATTCTATCACTTACAAATCTGATGGGAGCTATCTAATTGAGACAGAAGAAGACATTTCCCCACCTTTGGAGGTTAAAGCAACTTGTGTAGGTGATCATGATTTCAGAGTTGAAGCTGATGGTGTAATCATGGATGTTAGCTTAGCTGTTTACTCGAAGGATCAGACCAAGCACATTCATCTGTGGCATGGGTCACATCATCATCATTATAGACAAAAATTGGGTCTTGAATTGTCCGATGAAGATGAAACCGAACATAGGCCCAGTTTTGATAGATCATCTCATCCTCCGGGAACAGTTGTTGCACCCATGGCTGGTTTGGTGGTCAAGGTTCTTATTAAGGATGCAACAAGAGTGGAGGAAGGACAGCCTATTTTAGTATTAGAGGCAATGAAGATGGAGCATGTTGTAAAGGCACCATCAGCAGGCTATGTCCACGGGCTTCATCTAGCAGCTGGACAGCAGGTTTCTGATGGCAGTGTTCTCTTCAGTATTAAGAACAAATATATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004485 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0005840 GO:0006082 GO:0006520 GO:0006551 GO:0006552 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009507 GO:0009536 GO:0009987 GO:0016054 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022626 GO:0031974 GO:0032991 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046395 GO:0046872 GO:0046914 GO:0050897 GO:0070013 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00280 ko01100 map00280 map01100

Pfam Domains

Protein Families

Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2

Protein Analysis

735

Amino Acids

81.12

Weight (kDa)

6.32

Isoelectric Point (pI)

41.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_carb_N	PF00289	34 - 142	3.1e-45	Biotin carboxylase, N-terminal domain
CPSase_L_D2	PF02786	147 - 355	1.9e-74	Carbamoyl-phosphate synthase L chain, ATP binding domain
Dala_Dala_lig_C	PF07478	174 - 324	4.2e-10	D-ala D-ala ligase C-terminus
Biotin_carb_C	PF02785	369 - 476	5.9e-36	Biotin carboxylase C-terminal domain
MCCA_BT	PF19331	489 - 652	7.3e-80	Methylcrotonoyl-CoA carboxylase subunit alpha BT domain
Biotin_lipoyl	PF00364	664 - 730	1.3e-15	Biotin-requiring enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011225)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03090 AT1G03090
fragaria_vesca	FvH4_2g23110 FvH4_2g23110 FvH4_2g23110 FvH4_2g23110
malus_domestica	MD11G1280500.v1.1
prunus_persica	Prupe.8G236400_v2.0.a1
pyrus_communis	pycom11g24820
rosa_chinensis	RchiOBHm_Chr6g0290331
rosa_laevigata	RLG00000012226
rosa_multiflora	Rmu_sc0013786.1_g000003
rosa_roxburghii	Rroxscaffold_7G00177150
rosa_rugosa	Rorug06G0211900
rosa_samantha	Rh6AG322200 Rh6BG329500 Rh6CG335400 Rh6DG322400
rosa_wichuraiana	Rw6G027820 Rw6G027920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1331
AasI	GACNNNNNNGTC	1 cut(s)	1448
Acc16I	TGCGCA	1 cut(s)	321
Acc36I	ACCTGC	2 cut(s)	1331, 2149
Acc65I	GGTACC	1 cut(s)	478
AccB1I	GGYRCC	3 cut(s)	478, 798, 2110
AccB7I	CCANNNNNTGG	1 cut(s)	578
AccIII	TCCGGA	1 cut(s)	616
AciI	CCGC	8 cut(s)	25, 92, 156, 228, 303, 869, 1517, 1607
AclWI	GGATC	3 cut(s)	152, 1244, 1863
AcoI	YGGCCR	2 cut(s)	12, 1012
AcsI	RAATTY	5 cut(s)	171, 920, 1035, 1391, 1543
AcuI	CTGAAG	3 cut(s)	962, 1331, 2167
AcyI	GRCGYC	3 cut(s)	5, 198, 300
AdeI	CACNNNGTG	1 cut(s)	653
AfaI	GTAC	5 cut(s)	313, 480, 789, 1131, 1347
AgsI	TTSAA	9 cut(s)	176, 632, 1106, 1220, 1397, 1427, 1535, 1808, 1925
AhdI	GACNNNNNGTC	1 cut(s)	850
AjiI	CACGTC	1 cut(s)	220
AjnI	CCWGG	3 cut(s)	334, 490, 1567
AjuI	GAANNNNNNNTTGG	2 cut(s)	624, 656
Alw21I	GWGCWC	1 cut(s)	649
Alw26I	GTCTC	7 cut(s)	157, 858, 943, 1202, 1221, 1532, 1730
AlwI	GGATC	3 cut(s)	152, 1244, 1863
AlwNI	CAGNNNCTG	1 cut(s)	419
Ama87I	CYCGRG	1 cut(s)	1649
Aor13HI	TCCGGA	1 cut(s)	616
AoxI	GGCC	6 cut(s)	12, 237, 407, 943, 1012, 1955
ApeKI	GCWGC	8 cut(s)	470, 659, 662, 783, 881, 1493, 1520, 2147
ApoI	RAATTY	5 cut(s)	171, 920, 1035, 1391, 1543
ArsI	GACNNNNNNTTYG	2 cut(s)	1903, 1935
Asp700I	GAANNNNTTC	2 cut(s)	1059, 1563
Asp718I	GGTACC	1 cut(s)	478
AspLEI	GCGC	1 cut(s)	322
AspS9I	GGNCC	5 cut(s)	153, 251, 407, 1081, 1956
AsuC2I	CCSGG	2 cut(s)	125, 1986
AsuHPI	GGTGA	3 cut(s)	449, 1182, 1799
AvaI	CYCGRG	1 cut(s)	1649
AvaII	GGWCC	3 cut(s)	153, 251, 1081
BalI	TGGCCA	2 cut(s)	14, 1014
BanI	GGYRCC	3 cut(s)	478, 798, 2110
BbsI	GAAGAC	2 cut(s)	968, 1752
Bbv12I	GWGCWC	1 cut(s)	649
BbvI	GCAGC	8 cut(s)	482, 646, 649, 795, 868, 1480, 1507, 2159
BccI	CCATC	9 cut(s)	339, 431, 1201, 1392, 1712, 1808, 2086, 2122, 2162
BceAI	ACGGC	3 cut(s)	170, 224, 290
BciT130I	CCWGG	3 cut(s)	336, 492, 1569
BclI	TGATCA	1 cut(s)	1789
BcnI	CCSGG	2 cut(s)	125, 1986
BcoDI	GTCTC	7 cut(s)	157, 858, 943, 1202, 1221, 1532, 1730
BfaI	CTAG	4 cut(s)	1458, 1497, 1632, 2144
BfuAI	ACCTGC	2 cut(s)	1331, 2149
BglI	GCCNNNNNGGC	2 cut(s)	236, 1514
BglII	AGATCT	1 cut(s)	1018
BlpI	GCTNAGC	2 cut(s)	269, 1837
Bme1390I	CCNGG	5 cut(s)	125, 336, 492, 1569, 1986
Bme18I	GGWCC	3 cut(s)	153, 251, 1081
BmeRI	GACNNNNNGTC	1 cut(s)	850
BmeT110I	CYCGRG	1 cut(s)	1649
BmgBI	CACGTC	1 cut(s)	220
BmgT120I	GGNCC	5 cut(s)	153, 251, 407, 1081, 1956
BmiI	GGNNCC	6 cut(s)	258, 480, 800, 1083, 1142, 2112
BmrFI	CCNGG	5 cut(s)	125, 336, 492, 1569, 1986
BmrI	ACTGGG	1 cut(s)	1953
BmsI	GCATC	3 cut(s)	425, 997, 2029
BmuI	ACTGGG	1 cut(s)	1953
BpiI	GAAGAC	2 cut(s)	968, 1752
BpmI	CTGGAG	5 cut(s)	396, 713, 1167, 1176, 1233
Bpu1102I	GCTNAGC	2 cut(s)	269, 1837
BpuEI	CTTGAG	1 cut(s)	1666
BpuMI	CCSGG	2 cut(s)	125, 1986
BsaBI	GATNNNNATC	1 cut(s)	519
BsaHI	GRCGYC	3 cut(s)	5, 198, 300
BsaI	GGTCTC	1 cut(s)	858
BsaJI	CCNNGG	5 cut(s)	124, 640, 885, 2004, 2131
BsaWI	WCCGGW	2 cut(s)	616, 849
BsaXI	ACNNNNNCTCC	8 cut(s)	54, 84, 198, 228, 1218, 1248, 1571, 1601
Bse118I	RCCGGY	1 cut(s)	908
Bse1I	ACTGG	7 cut(s)	319, 1159, 1184, 1216, 1305, 1518, 1959
Bse3DI	GCAATG	1 cut(s)	2091
Bse8I	GATNNNNATC	1 cut(s)	519
BseAI	TCCGGA	1 cut(s)	616
BseBI	CCWGG	3 cut(s)	336, 492, 1569
BseDI	CCNNGG	5 cut(s)	124, 640, 885, 2004, 2131
BseGI	GGATG	6 cut(s)	331, 625, 988, 1834, 1978, 2044
BseJI	GATNNNNATC	1 cut(s)	519
BseMI	GCAATG	1 cut(s)	2091
BseMII	CTCAG	3 cut(s)	283, 954, 990
BseNI	ACTGG	7 cut(s)	319, 1159, 1184, 1216, 1305, 1518, 1959
BseXI	GCAGC	8 cut(s)	482, 646, 649, 795, 868, 1480, 1507, 2159
BseYI	CCCAGC	1 cut(s)	823
BsgI	GTGCAG	3 cut(s)	160, 489, 1311
BshFI	GGCC	6 cut(s)	14, 239, 409, 945, 1014, 1957
BshNI	GGYRCC	3 cut(s)	478, 798, 2110
BsiHKAI	GWGCWC	1 cut(s)	649
BsiHKCI	CYCGRG	1 cut(s)	1649
BsiSI	CCGG	9 cut(s)	28, 39, 124, 254, 260, 617, 850, 909, 1985
BslFI	GGGAC	2 cut(s)	443, 1067
BsmAI	GTCTC	7 cut(s)	157, 858, 943, 1202, 1221, 1532, 1730
BsmFI	GGGAC	2 cut(s)	443, 1067
BsnI	GGCC	6 cut(s)	14, 239, 409, 945, 1014, 1957
Bso31I	GGTCTC	1 cut(s)	858
BsoBI	CYCGRG	1 cut(s)	1649
Bsp1286I	GDGCHC	1 cut(s)	649
Bsp13I	TCCGGA	1 cut(s)	616
Bsp143I	GATC	9 cut(s)	74, 144, 520, 1018, 1249, 1584, 1789, 1855, 1970
Bsp1720I	GCTNAGC	2 cut(s)	269, 1837
Bsp19I	CCATGG	1 cut(s)	2004
BspACI	CCGC	8 cut(s)	25, 92, 156, 228, 303, 869, 1517, 1607
BspANI	GGCC	6 cut(s)	14, 239, 409, 945, 1014, 1957
BspCNI	CTCAG	3 cut(s)	282, 953, 991
BspEI	TCCGGA	1 cut(s)	616
BspHI	TCATGA	2 cut(s)	147, 1792
BspLI	GGNNCC	6 cut(s)	258, 480, 800, 1083, 1142, 2112
BspMI	ACCTGC	2 cut(s)	1331, 2149
BspPI	GGATC	3 cut(s)	152, 1244, 1863
BspT107I	GGYRCC	3 cut(s)	478, 798, 2110
BspTNI	GGTCTC	1 cut(s)	858
BsrDI	GCAATG	1 cut(s)	2091
BsrFI	RCCGGY	1 cut(s)	908
BsrI	ACTGG	7 cut(s)	319, 1159, 1184, 1216, 1305, 1518, 1959
BssAI	RCCGGY	1 cut(s)	908
BssECI	CCNNGG	5 cut(s)	124, 640, 885, 2004, 2131
BssMI	GATC	9 cut(s)	74, 144, 520, 1018, 1249, 1584, 1789, 1855, 1970
BssNI	GRCGYC	3 cut(s)	5, 198, 300
BssT1I	CCWWGG	3 cut(s)	640, 885, 2004
Bst2UI	CCWGG	3 cut(s)	336, 492, 1569
Bst4CI	ACNGT	8 cut(s)	19, 181, 905, 916, 937, 1232, 1483, 1993
Bst6I	CTCTTC	1 cut(s)	2186
BstACI	GRCGYC	3 cut(s)	5, 198, 300
BstC8I	GCNNGC	7 cut(s)	44, 139, 324, 910, 1179, 1241, 2122
BstDEI	CTNAG	4 cut(s)	269, 940, 999, 1837
BstDSI	CCRYGG	2 cut(s)	2004, 2131
BstENI	CCTNNNNNAGG	1 cut(s)	1089
BstF5I	GGATG	6 cut(s)	331, 625, 988, 1834, 1978, 2044
BstHHI	GCGC	1 cut(s)	322
BstKTI	GATC	9 cut(s)	77, 147, 523, 1021, 1252, 1587, 1792, 1858, 1973
BstMAI	GTCTC	7 cut(s)	157, 858, 943, 1202, 1221, 1532, 1730
BstMBI	GATC	9 cut(s)	74, 144, 520, 1018, 1249, 1584, 1789, 1855, 1970
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 236, 878, 887, 1106, 1514, 2006, 2117
BstNI	CCWGG	3 cut(s)	336, 492, 1569
BstNSI	RCATGY	4 cut(s)	1181, 1243, 1527, 2102
BstSCI	CCNGG	5 cut(s)	123, 334, 490, 1567, 1984
BstV1I	GCAGC	8 cut(s)	482, 646, 649, 795, 868, 1480, 1507, 2159
BstV2I	GAAGAC	2 cut(s)	968, 1752
BstX2I	RGATCY	1 cut(s)	1018
BstXI	CCANNNNNNTGG	1 cut(s)	737
BstYI	RGATCY	1 cut(s)	1018
BsuRI	GGCC	6 cut(s)	14, 239, 409, 945, 1014, 1957
BtgI	CCRYGG	2 cut(s)	2004, 2131
BtrI	CACGTC	1 cut(s)	220
BtsCI	GGATG	6 cut(s)	331, 625, 988, 1834, 1978, 2044
BtsI	GCAGTG	2 cut(s)	1681, 2179
BtsIMutI	CAGTG	8 cut(s)	15, 910, 921, 1237, 1298, 1488, 1681, 2179
BveI	ACCTGC	2 cut(s)	1331, 2149
Cac8I	GCNNGC	7 cut(s)	44, 139, 324, 910, 1179, 1241, 2122
CaiI	CAGNNNCTG	1 cut(s)	419
CciI	TCATGA	2 cut(s)	147, 1792
CfoI	GCGC	1 cut(s)	322
Cfr10I	RCCGGY	1 cut(s)	908
Cfr13I	GGNCC	5 cut(s)	153, 251, 407, 1081, 1956
CpoI	CGGWCCG	1 cut(s)	251
CseI	GACGC	3 cut(s)	206, 308, 438
Csp6I	GTAC	5 cut(s)	312, 479, 788, 1130, 1346
CspCI	CAANNNNNGTGG	2 cut(s)	2031, 2066
CspI	CGGWCCG	1 cut(s)	251
CviQI	GTAC	5 cut(s)	312, 479, 788, 1130, 1346
DdeI	CTNAG	4 cut(s)	269, 940, 999, 1837
DpnI	GATC	9 cut(s)	76, 146, 522, 1020, 1251, 1586, 1791, 1857, 1972
DpnII	GATC	9 cut(s)	74, 144, 520, 1018, 1249, 1584, 1789, 1855, 1970
DraI	TTTAAA	1 cut(s)	1435
DraIII	CACNNNGTG	1 cut(s)	653
DrdI	GACNNNNNNGTC	1 cut(s)	1448
DriI	GACNNNNNGTC	1 cut(s)	850
DseDI	GACNNNNNNGTC	1 cut(s)	1448
EaeI	YGGCCR	2 cut(s)	12, 1012
Eam1104I	CTCTTC	1 cut(s)	2186
Eam1105I	GACNNNNNGTC	1 cut(s)	850
EarI	CTCTTC	1 cut(s)	2186
EciI	GGCGGA	3 cut(s)	14, 217, 1596
Eco130I	CCWWGG	3 cut(s)	640, 885, 2004
Eco31I	GGTCTC	1 cut(s)	858
Eco32I	GATATC	2 cut(s)	497, 554
Eco47I	GGWCC	3 cut(s)	153, 251, 1081
Eco57I	CTGAAG	3 cut(s)	962, 1331, 2167
Eco88I	CYCGRG	1 cut(s)	1649
EcoNI	CCTNNNNNAGG	1 cut(s)	1089
EcoO109I	RGGNCCY	1 cut(s)	1081
EcoRI	GAATTC	1 cut(s)	171
EcoRII	CCWGG	3 cut(s)	334, 490, 1567
EcoRV	GATATC	2 cut(s)	497, 554
EcoT14I	CCWWGG	3 cut(s)	640, 885, 2004
EcoT22I	ATGCAT	1 cut(s)	1245
ErhI	CCWWGG	3 cut(s)	640, 885, 2004
FalI	AAGNNNNNCTT	6 cut(s)	624, 656, 1128, 1160, 1304, 1336
FaqI	GGGAC	2 cut(s)	443, 1067
FauNDI	CATATG	1 cut(s)	1592
FbaI	TGATCA	1 cut(s)	1789
FokI	GGATG	6 cut(s)	318, 632, 975, 1841, 1965, 2051
FspBI	CTAG	4 cut(s)	1458, 1497, 1632, 2144
FspI	TGCGCA	1 cut(s)	321
GlaI	GCGC	1 cut(s)	321
GsaI	CCCAGC	1 cut(s)	827
GsuI	CTGGAG	5 cut(s)	396, 713, 1167, 1176, 1233
HaeIII	GGCC	6 cut(s)	14, 239, 409, 945, 1014, 1957
HapII	CCGG	9 cut(s)	28, 39, 124, 254, 260, 617, 850, 909, 1985
HgaI	GACGC	3 cut(s)	206, 308, 438
HhaI	GCGC	1 cut(s)	322
Hin1I	GRCGYC	3 cut(s)	5, 198, 300
Hin6I	GCGC	1 cut(s)	320
HinP1I	GCGC	1 cut(s)	320
HincII	GTYRAC	1 cut(s)	1453
HindII	GTYRAC	1 cut(s)	1453
HindIII	AAGCTT	1 cut(s)	1265
HinfI	GANTC	6 cut(s)	86, 99, 109, 165, 292, 1617
HpaII	CCGG	9 cut(s)	28, 39, 124, 254, 260, 617, 850, 909, 1985
HphI	GGTGA	3 cut(s)	449, 1182, 1799
Hpy166II	GTNNAC	5 cut(s)	679, 1346, 1453, 1846, 2131
Hpy188III	TCNNGA	8 cut(s)	103, 148, 296, 617, 730, 1388, 1793, 1922
Hpy8I	GTNNAC	5 cut(s)	679, 1346, 1453, 1846, 2131
Hpy99I	CGWCG	4 cut(s)	185, 200, 236, 292
HpyAV	CCTTC	7 cut(s)	646, 789, 1070, 1154, 1306, 1846, 2051
HpyCH4III	ACNGT	8 cut(s)	19, 181, 905, 916, 937, 1232, 1483, 1993
HpyCH4IV	ACGT	2 cut(s)	219, 652
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 236, 878, 887, 1106, 1514, 2006, 2117
HpyF3I	CTNAG	4 cut(s)	269, 940, 999, 1837
HpySE526I	ACGT	2 cut(s)	219, 652
Hsp92I	GRCGYC	3 cut(s)	5, 198, 300
HspAI	GCGC	1 cut(s)	320
Kpn2I	TCCGGA	1 cut(s)	616
KpnI	GGTACC	1 cut(s)	482
KroI	GCCGGC	1 cut(s)	908
KroNI	GCCGGC	1 cut(s)	910
Ksp22I	TGATCA	1 cut(s)	1789
Kzo9I	GATC	9 cut(s)	74, 144, 520, 1018, 1249, 1584, 1789, 1855, 1970
LmnI	GCTCC	7 cut(s)	219, 262, 644, 832, 1186, 1722, 2095
Lsp1109I	GCAGC	8 cut(s)	482, 646, 649, 795, 868, 1480, 1507, 2159
LweI	GCATC	3 cut(s)	425, 997, 2029
MaeI	CTAG	4 cut(s)	1458, 1497, 1632, 2144
MaeII	ACGT	2 cut(s)	219, 652
MaeIII	GTNAC	3 cut(s)	994, 1074, 1887
MalI	GATC	9 cut(s)	76, 146, 522, 1020, 1251, 1586, 1791, 1857, 1972
MboI	GATC	9 cut(s)	74, 144, 520, 1018, 1249, 1584, 1789, 1855, 1970
MflI	RGATCY	1 cut(s)	1018
MhlI	GDGCHC	1 cut(s)	649
MlsI	TGGCCA	2 cut(s)	14, 1014
MluNI	TGGCCA	2 cut(s)	14, 1014
MlyI	GAGTC	3 cut(s)	80, 159, 1626
MmeI	TCCRAC	2 cut(s)	108, 387
Mox20I	TGGCCA	2 cut(s)	14, 1014
Mph1103I	ATGCAT	1 cut(s)	1245
MroI	TCCGGA	1 cut(s)	616
MroNI	GCCGGC	1 cut(s)	908
MroXI	GAANNNNTTC	2 cut(s)	1059, 1563
MscI	TGGCCA	2 cut(s)	14, 1014
MseI	TTAA	6 cut(s)	563, 1434, 1770, 2034, 2190, 2206
MslI	CAYNNNNRTG	4 cut(s)	582, 753, 768, 962
Msp20I	TGGCCA	2 cut(s)	14, 1014
MspA1I	CMGCKG	1 cut(s)	2150
MspI	CCGG	9 cut(s)	28, 39, 124, 254, 260, 617, 850, 909, 1985
MspR9I	CCNGG	5 cut(s)	125, 336, 492, 1569, 1986
MvaI	CCWGG	3 cut(s)	336, 492, 1569
MwoI	GCNNNNNNNGC	8 cut(s)	11, 236, 878, 887, 1106, 1514, 2006, 2117
NaeI	GCCGGC	1 cut(s)	910
NciI	CCSGG	2 cut(s)	125, 1986
NcoI	CCATGG	1 cut(s)	2004
NdeI	CATATG	1 cut(s)	1592
NdeII	GATC	9 cut(s)	74, 144, 520, 1018, 1249, 1584, 1789, 1855, 1970
NgoMIV	GCCGGC	1 cut(s)	908
NlaIV	GGNNCC	6 cut(s)	258, 480, 800, 1083, 1142, 2112
NmeAIII	GCCGAG	1 cut(s)	244
NmuCI	GTSAC	3 cut(s)	994, 1074, 1887
NsbI	TGCGCA	1 cut(s)	321
NsiI	ATGCAT	1 cut(s)	1245
NspI	RCATGY	4 cut(s)	1181, 1243, 1527, 2102
PaeI	GCATGC	2 cut(s)	1181, 1243
PaeR7I	CTCGAG	1 cut(s)	1649
PagI	TCATGA	2 cut(s)	147, 1792
PaqCI	CACCTGC	1 cut(s)	1331
PdiI	GCCGGC	1 cut(s)	910
PdmI	GAANNNNTTC	2 cut(s)	1059, 1563
PfeI	GAWTC	3 cut(s)	99, 109, 292
PflMI	CCANNNNNTGG	1 cut(s)	578
PfoI	TCCNGGA	2 cut(s)	1567, 1984
PleI	GAGTC	3 cut(s)	80, 159, 1625
PpsI	GAGTC	3 cut(s)	80, 159, 1625
PpuMI	RGGWCCY	1 cut(s)	1081
Psp5II	RGGWCCY	1 cut(s)	1081
Psp6I	CCWGG	3 cut(s)	334, 490, 1567
PspFI	CCCAGC	1 cut(s)	823
PspGI	CCWGG	3 cut(s)	334, 490, 1567
PspN4I	GGNNCC	6 cut(s)	258, 480, 800, 1083, 1142, 2112
PspPI	GGNCC	5 cut(s)	153, 251, 407, 1081, 1956
PspPPI	RGGWCCY	1 cut(s)	1081
PspXI	VCTCGAGB	1 cut(s)	1649
PstNI	CAGNNNCTG	1 cut(s)	419
PsuI	RGATCY	1 cut(s)	1018
PvuII	CAGCTG	1 cut(s)	2150
RsaI	GTAC	5 cut(s)	313, 480, 789, 1131, 1347
RsaNI	GTAC	5 cut(s)	312, 479, 788, 1130, 1346
RseI	CAYNNNNRTG	4 cut(s)	582, 753, 768, 962
Rsr2I	CGGWCCG	1 cut(s)	251
RsrII	CGGWCCG	1 cut(s)	251
SaqAI	TTAA	6 cut(s)	563, 1434, 1770, 2034, 2190, 2206
Sau3AI	GATC	9 cut(s)	74, 144, 520, 1018, 1249, 1584, 1789, 1855, 1970
Sau96I	GGNCC	5 cut(s)	153, 251, 407, 1081, 1956
SchI	GAGTC	3 cut(s)	80, 159, 1626
ScrFI	CCNGG	5 cut(s)	125, 336, 492, 1569, 1986
SduI	GDGCHC	1 cut(s)	649
SfaNI	GCATC	3 cut(s)	425, 997, 2029
Sfr274I	CTCGAG	1 cut(s)	1649
SinI	GGWCC	3 cut(s)	153, 251, 1081
SlaI	CTCGAG	1 cut(s)	1649
SmiMI	CAYNNNNRTG	4 cut(s)	582, 753, 768, 962
SmlI	CTYRAG	2 cut(s)	1649, 1681
SmoI	CTYRAG	2 cut(s)	1649, 1681
SphI	GCATGC	2 cut(s)	1181, 1243
SsiI	CCGC	8 cut(s)	25, 92, 156, 228, 303, 869, 1517, 1607
SspMI	CTAG	4 cut(s)	1458, 1497, 1632, 2144
StyD4I	CCNGG	5 cut(s)	123, 334, 490, 1567, 1984
StyI	CCWWGG	3 cut(s)	640, 885, 2004
TaaI	ACNGT	8 cut(s)	19, 181, 905, 916, 937, 1232, 1483, 1993
TaiI	ACGT	2 cut(s)	222, 655
TaqI	TCGA	7 cut(s)	102, 290, 813, 930, 1201, 1650, 1850
TaqII	GACCGA	1 cut(s)	239
TauI	GCSGC	4 cut(s)	94, 305, 872, 1520
TfiI	GAWTC	3 cut(s)	99, 109, 292
Tru1I	TTAA	6 cut(s)	563, 1434, 1770, 2034, 2190, 2206
Tru9I	TTAA	6 cut(s)	563, 1434, 1770, 2034, 2190, 2206
TscAI	CASTG	8 cut(s)	15, 910, 921, 1237, 1305, 1488, 1681, 2179
TseFI	GTSAC	3 cut(s)	994, 1074, 1887
TseI	GCWGC	8 cut(s)	470, 659, 662, 783, 881, 1493, 1520, 2147
Tsp45I	GTSAC	3 cut(s)	994, 1074, 1887
TspGWI	ACGGA	1 cut(s)	41
TspRI	CASTG	8 cut(s)	15, 910, 921, 1237, 1305, 1488, 1681, 2179
Van91I	CCANNNNNTGG	1 cut(s)	578
VpaK11BI	GGWCC	3 cut(s)	153, 251, 1081
XagI	CCTNNNNNAGG	1 cut(s)	1089
XapI	RAATTY	5 cut(s)	171, 920, 1035, 1391, 1543
XceI	RCATGY	4 cut(s)	1181, 1243, 1527, 2102
XcmI	CCANNNNNNNNNTGG	3 cut(s)	338, 430, 2011
XhoI	CTCGAG	1 cut(s)	1649
XmnI	GAANNNNTTC	2 cut(s)	1059, 1563
XspI	CTAG	4 cut(s)	1458, 1497, 1632, 2144
Zsp2I	ATGCAT	1 cut(s)	1245

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.