Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0011225

Physical Location & Seq

Genomic Coordinates Forward (+)

18965781 .. 18971831

Gene Structure 6051 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g23110.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2205 bp

ATGGCGTCAGTCGCCACCGTCCTCCGCCGTAACCTCTCCGGCAAGCCACTCCGTTTCCGACTTCCATCACCACGATCATTCTCCGACTCGCCGCCGCAGAGACTCGAGAAGATTCTGATCGCCAACCGCGGCGAAATCGCCTGCAGGATCATGCGGACCGCGAAGCGGCTCGGAATTCAAACCGTCGCCGTTTTCAGCGACGCCGATAGATACTCGCTCCACGTCAAATCCGCCGACGAGGCCGTTCGTATCGGTCCGGCTCCGGCTCGGCTCAGCTACCTCAACGCCTCGTCGATTCTCGACGCCGCCGTTCGCACCGGTGCGCAGGCTATCCATCCTGGCTATGGTTTTCTGTCAGAGAGTTCTGATTTTGCGCAACTTTGTGAAGATAAGGGTCTCACCTTTATTGGACCTCCGGCATCTGCCATAAGGGACATGGGGGATAAGAGTGCGTCAAAGAGGATAATGGGTGCAGCAGGGGTACCTCTTGTGCCTGGATATCATGGTCACGATCAAGATATTGAATTAATGAAGATGGAAGCTGATAAGATTGGATATCCAATCTTAATAAAGCCAACTCATGGAGGTGGGGGAAAGGGTATGAGGATTGTGCAAAGTCCGGATGAGTTTGTTGAAGCCTTTCTTGGAGCACAACGTGAGGCTGCTGCTTCTTTTGGCATAAACACAATTTTGCTGGAGAAATATATTACACAGCCAAGGCACATAGAAGTCCAGATATTTGGGGATAAACACGGGAATGTTCTACATTTATATGAGAGAGATTGCAGTGTACAAAGAAGACACCAGAAAATAATTGAAGAAGCTCCAGCTCCAAATGTTTCTGAAGACTTCCGGTCCCACGTGGGTCAAGCGGCTGTATCTGCTGCCAAGGCAGTTGGCTATCACAGTGCTGGCACAGTGGAGTTTATAGTGGATACAGTCTCAGGCCAGTTCTATTTCATGGAGATGAATACCCGTCTTCAGGTTGAGCATCCTGTGACCGAGATGATTGTTGGCCAAGATCTTGTAGAGTGGCAAATTCGTGTAGCAAATGGAGAACATCTTCCCATTAGTCAGTCACAAGTCCCGTTGTCCGGTCATGCTTTTGAAGCCCGGATATATGCTGAAAATGTACCAAAAGGATTCCTTCCTGCAACTGGAGTTCTTCATCACTATCAGCATGCTCCCCTTTCACCAACAGTTCGAGTTGAGACTGGAGTTGAACAAGGAGACACCGTTAGCATGCATTATGATCCTATGATTGCAAAGCTTGTAGTATGGGGAGAAAATCGTGCAGTAGCACTGCTAAAACTGAAGGATTGCTTGACAAAGTTTCAGGTTGCAGGTGTACCAACCAACATCAGTTTTCTTTTCAAACTTGCCAATCATCGGGAATTTGAAAATGGGAATGTGGAAACTCATTTTATTGAACATTTTAAAGATGACCTATTCCCTAGTAATTTGGAAGTAACAAACACAGTGCTTGGTGCTGCTAGATTTGGTGCCAAACTGGCAGCTGCGTGTCTCATTGAAAAGGAGAATTCTGTATTTAGAGAAAATCTTCCTGGAAGCAACACGATCTCGATATGGTATTCTAATCCACCGTTCAGAGTCCATCATTGTGCTAGGCATACACTGGAACTTGAGTGGGAGAATGAATACGATAGCAGTGGCTCAAAGTTATTGACCTTTTCAGTAACTTACAAATCAGATGGGAGCTATTTAATTGAGACAGAAGAGGACATTTCCCCACCTTTGGAGGTCAAAGCAACTTGTGTAGGTGACCATGATTTCAGAGTTGAAGCTGATGATGTAATCATGGATGTTAGCTTAGCTGTTTACTCGAAGGATCAGACCAAGCACATTCATATGTGGCATGGGTCCCATCATCATCATTTCAGACAAAAATTGGGTCTTGAATTATCCAATGAAGATGAAACCGAACATAGGCCCAGTTTTGACAGATCATCTCATCCTCCGGGGACTGTTGTTGCACCCATGGCAGGTTTGGTTGTCAAGGTTCTTATTAGAGATGCAACAAAAGTGGAGGAAGGACAACCTATTTTAGTATTAGAGGCAATGAAGATGGAGCATGTTGTAAAGGCACCATCAGCAGGCTATGTCCACGGGCTTCATCTATCAGCTGGCGAGCAGGTTTCTGATGGCAGTGTTCTCTTCAGTATTAAGGAGAAGGATTCACAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004485 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0005840 GO:0006082 GO:0006520 GO:0006551 GO:0006552 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009507 GO:0009536 GO:0009987 GO:0016054 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022626 GO:0031974 GO:0032991 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046395 GO:0046872 GO:0046914 GO:0050897 GO:0070013 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00280 ko01100 map00280 map01100

Pfam Domains

Protein Families

Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2

Protein Analysis

735

Amino Acids

81.02

Weight (kDa)

6.11

Isoelectric Point (pI)

38.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_L_D2	PF02786	3 - 211	1.1e-74	Carbamoyl-phosphate synthase L chain, ATP binding domain
Dala_Dala_lig_C	PF07478	28 - 180	6.9e-11	D-ala D-ala ligase C-terminus
Biotin_carb_C	PF02785	225 - 332	1.5e-35	Biotin carboxylase C-terminal domain
MCCA_BT	PF19331	343 - 505	4.7e-79	Methylcrotonoyl-CoA carboxylase subunit alpha BT domain
Biotin_lipoyl	PF00364	517 - 583	1.2e-15	Biotin-requiring enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011225)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03090 AT1G03090
fragaria_vesca	FvH4_2g23110 FvH4_2g23110 FvH4_2g23110 FvH4_2g23110
malus_domestica	MD11G1280500.v1.1
prunus_persica	Prupe.8G236400_v2.0.a1
pyrus_communis	pycom11g24820
rosa_chinensis	RchiOBHm_Chr6g0290331
rosa_laevigata	RLG00000012226
rosa_multiflora	Rmu_sc0013786.1_g000003
rosa_roxburghii	Rroxscaffold_7G00177150
rosa_rugosa	Rorug06G0211900
rosa_samantha	Rh6AG322200 Rh6BG329500 Rh6CG335400 Rh6DG322400
rosa_wichuraiana	Rw6G027820 Rw6G027920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1334
Acc16I	TGCGCA	2 cut(s)	324, 375
Acc36I	ACCTGC	3 cut(s)	1334, 1994, 2143
Acc65I	GGTACC	1 cut(s)	481
AccB1I	GGYRCC	3 cut(s)	481, 1502, 2104
AccB7I	CCANNNNNTGG	1 cut(s)	581
AccII	CGCG	2 cut(s)	129, 161
AccIII	TCCGGA	1 cut(s)	619
AclWI	GGATC	3 cut(s)	155, 1247, 1857
AcoI	YGGCCR	1 cut(s)	1015
AcsI	RAATTY	4 cut(s)	174, 1038, 1394, 1540
AcuI	CTGAAG	4 cut(s)	864, 965, 1334, 2161
AcvI	CACGTG	1 cut(s)	862
AcyI	GRCGYC	3 cut(s)	5, 201, 303
AdeI	CACNNNGTG	1 cut(s)	656
AfaI	GTAC	4 cut(s)	483, 792, 1134, 1350
AgeI	ACCGGT	1 cut(s)	317
AhdI	GACNNNNNGTC	1 cut(s)	853
AjiI	CACGTC	1 cut(s)	223
AjnI	CCWGG	3 cut(s)	337, 493, 1564
AjuI	GAANNNNNNNTTGG	2 cut(s)	627, 659
Alw21I	GWGCWC	1 cut(s)	652
Alw26I	GTCTC	7 cut(s)	94, 401, 946, 1205, 1224, 1529, 1724
AlwI	GGATC	3 cut(s)	155, 1247, 1857
Ama87I	CYCGRG	1 cut(s)	104
Aor13HI	TCCGGA	1 cut(s)	619
AoxI	GGCC	4 cut(s)	240, 946, 1015, 1949
ApeKI	GCWGC	7 cut(s)	473, 662, 665, 884, 1490, 1514, 1517
ApoI	RAATTY	4 cut(s)	174, 1038, 1394, 1540
ArsI	GACNNNNNNTTYG	2 cut(s)	1897, 1929
AseI	ATTAAT	1 cut(s)	527
AsiGI	ACCGGT	1 cut(s)	317
Asp700I	GAANNNNTTC	3 cut(s)	639, 1062, 1560
Asp718I	GGTACC	1 cut(s)	481
AspLEI	GCGC	2 cut(s)	325, 376
AspS9I	GGNCC	6 cut(s)	156, 254, 410, 855, 1881, 1950
AsuC2I	CCSGG	2 cut(s)	1114, 1980
AsuHPI	GGTGA	4 cut(s)	60, 391, 1185, 1793
AvaI	CYCGRG	1 cut(s)	104
AvaII	GGWCC	5 cut(s)	156, 254, 410, 855, 1881
BalI	TGGCCA	1 cut(s)	1017
BanI	GGYRCC	3 cut(s)	481, 1502, 2104
BbrPI	CACGTG	1 cut(s)	862
BbsI	GAAGAC	3 cut(s)	805, 852, 971
Bbv12I	GWGCWC	1 cut(s)	652
BbvI	GCAGC	7 cut(s)	485, 649, 652, 871, 1477, 1504, 1526
BccI	CCATC	9 cut(s)	73, 342, 529, 1623, 1706, 1893, 2080, 2116, 2156
BceAI	ACGGC	4 cut(s)	12, 173, 227, 293
BciT130I	CCWGG	3 cut(s)	339, 495, 1566
BciVI	GTATCC	1 cut(s)	928
BcnI	CCSGG	2 cut(s)	1114, 1980
BcoDI	GTCTC	7 cut(s)	94, 401, 946, 1205, 1224, 1529, 1724
BfaI	CTAG	3 cut(s)	1455, 1494, 1626
BfmI	CTRYAG	1 cut(s)	142
BfuAI	ACCTGC	3 cut(s)	1334, 1994, 2143
BfuI	GTATCC	1 cut(s)	928
BglI	GCCNNNNNGGC	2 cut(s)	239, 1511
BglII	AGATCT	1 cut(s)	1021
BlpI	GCTNAGC	2 cut(s)	272, 1831
Bme1390I	CCNGG	5 cut(s)	339, 495, 1114, 1566, 1980
Bme18I	GGWCC	5 cut(s)	156, 254, 410, 855, 1881
BmeRI	GACNNNNNGTC	1 cut(s)	853
BmeT110I	CYCGRG	1 cut(s)	104
BmgBI	CACGTC	1 cut(s)	223
BmgT120I	GGNCC	6 cut(s)	156, 254, 410, 855, 1881, 1950
BmiI	GGNNCC	7 cut(s)	261, 483, 857, 1504, 1882, 1883, 2106
BmrFI	CCNGG	5 cut(s)	339, 495, 1114, 1566, 1980
BmrI	ACTGGG	1 cut(s)	1947
BmsI	GCATC	3 cut(s)	428, 1000, 2023
BmuI	ACTGGG	1 cut(s)	1947
BpiI	GAAGAC	3 cut(s)	805, 852, 971
BpmI	CTGGAG	4 cut(s)	716, 810, 1179, 1236
Bpu1102I	GCTNAGC	2 cut(s)	272, 1831
BpuEI	CTTGAG	1 cut(s)	1664
BpuMI	CCSGG	2 cut(s)	1114, 1980
BsaAI	YACGTR	1 cut(s)	862
BsaBI	GATNNNNATC	1 cut(s)	116
BsaHI	GRCGYC	3 cut(s)	5, 201, 303
BsaI	GGTCTC	1 cut(s)	401
BsaJI	CCNNGG	6 cut(s)	127, 716, 888, 1979, 1998, 2125
BsaWI	WCCGGW	4 cut(s)	317, 619, 852, 1094
BsaXI	ACNNNNNCTCC	2 cut(s)	1221, 1251
Bse118I	RCCGGY	1 cut(s)	317
Bse1I	ACTGG	6 cut(s)	949, 1162, 1219, 1515, 1641, 1953
Bse3DI	GCAATG	1 cut(s)	2085
Bse8I	GATNNNNATC	1 cut(s)	116
BseAI	TCCGGA	1 cut(s)	619
BseBI	CCWGG	3 cut(s)	339, 495, 1566
BseDI	CCNNGG	6 cut(s)	127, 716, 888, 1979, 1998, 2125
BseGI	GGATG	5 cut(s)	334, 628, 991, 1828, 1972
BseJI	GATNNNNATC	1 cut(s)	116
BseMI	GCAATG	1 cut(s)	2085
BseMII	CTCAG	2 cut(s)	286, 957
BseNI	ACTGG	6 cut(s)	949, 1162, 1219, 1515, 1641, 1953
BseXI	GCAGC	7 cut(s)	485, 649, 652, 871, 1477, 1504, 1526
BsgI	GTGCAG	2 cut(s)	492, 1314
Bsh1236I	CGCG	2 cut(s)	129, 161
BshFI	GGCC	4 cut(s)	242, 948, 1017, 1951
BshNI	GGYRCC	3 cut(s)	481, 1502, 2104
BshTI	ACCGGT	1 cut(s)	317
BsiHKAI	GWGCWC	1 cut(s)	652
BsiHKCI	CYCGRG	1 cut(s)	104
BslFI	GGGAC	5 cut(s)	446, 841, 1070, 1867, 1996
BsmAI	GTCTC	7 cut(s)	94, 401, 946, 1205, 1224, 1529, 1724
BsmFI	GGGAC	5 cut(s)	446, 841, 1070, 1867, 1996
BsnI	GGCC	4 cut(s)	242, 948, 1017, 1951
Bso31I	GGTCTC	1 cut(s)	401
BsoBI	CYCGRG	1 cut(s)	104
Bsp1286I	GDGCHC	1 cut(s)	652
Bsp13I	TCCGGA	1 cut(s)	619
Bsp1407I	TGTACA	1 cut(s)	790
Bsp143I	GATC	9 cut(s)	74, 117, 147, 511, 1021, 1252, 1578, 1849, 1964
Bsp1720I	GCTNAGC	2 cut(s)	272, 1831
Bsp19I	CCATGG	1 cut(s)	1998
BspANI	GGCC	4 cut(s)	242, 948, 1017, 1951
BspCNI	CTCAG	2 cut(s)	285, 956
BspEI	TCCGGA	1 cut(s)	619
BspFNI	CGCG	2 cut(s)	129, 161
BspLI	GGNNCC	7 cut(s)	261, 483, 857, 1504, 1882, 1883, 2106
BspMAI	CTGCAG	1 cut(s)	146
BspMI	ACCTGC	3 cut(s)	1334, 1994, 2143
BspPI	GGATC	3 cut(s)	155, 1247, 1857
BspT107I	GGYRCC	3 cut(s)	481, 1502, 2104
BspTNI	GGTCTC	1 cut(s)	401
BsrDI	GCAATG	1 cut(s)	2085
BsrFI	RCCGGY	1 cut(s)	317
BsrGI	TGTACA	1 cut(s)	790
BsrI	ACTGG	6 cut(s)	949, 1162, 1219, 1515, 1641, 1953
BssAI	RCCGGY	1 cut(s)	317
BssECI	CCNNGG	6 cut(s)	127, 716, 888, 1979, 1998, 2125
BssMI	GATC	9 cut(s)	74, 117, 147, 511, 1021, 1252, 1578, 1849, 1964
BssNI	GRCGYC	3 cut(s)	5, 201, 303
BssT1I	CCWWGG	3 cut(s)	716, 888, 1998
Bst2UI	CCWGG	3 cut(s)	339, 495, 1566
Bst6I	CTCTTC	2 cut(s)	1731, 2180
BstACI	GRCGYC	3 cut(s)	5, 201, 303
BstAUI	TGTACA	1 cut(s)	790
BstBAI	YACGTR	1 cut(s)	862
BstC8I	GCNNGC	9 cut(s)	44, 142, 327, 913, 1182, 1244, 2116, 2146, 2150
BstDEI	CTNAG	3 cut(s)	272, 943, 1831
BstDSI	CCRYGG	3 cut(s)	127, 1998, 2125
BstEII	GGTNACC	1 cut(s)	1781
BstF5I	GGATG	5 cut(s)	334, 628, 991, 1828, 1972
BstFNI	CGCG	2 cut(s)	129, 161
BstHHI	GCGC	2 cut(s)	325, 376
BstKTI	GATC	9 cut(s)	77, 120, 150, 514, 1024, 1255, 1581, 1852, 1967
BstMAI	GTCTC	7 cut(s)	94, 401, 946, 1205, 1224, 1529, 1724
BstMBI	GATC	9 cut(s)	74, 117, 147, 511, 1021, 1252, 1578, 1849, 1964
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 239, 881, 890, 1109, 1511, 2000, 2111
BstNI	CCWGG	3 cut(s)	339, 495, 1566
BstNSI	RCATGY	3 cut(s)	1184, 1246, 2096
BstPI	GGTNACC	1 cut(s)	1781
BstSCI	CCNGG	5 cut(s)	337, 493, 1112, 1564, 1978
BstSFI	CTRYAG	1 cut(s)	142
BstUI	CGCG	2 cut(s)	129, 161
BstV1I	GCAGC	7 cut(s)	485, 649, 652, 871, 1477, 1504, 1526
BstV2I	GAAGAC	3 cut(s)	805, 852, 971
BstX2I	RGATCY	1 cut(s)	1021
BstXI	CCANNNNNNTGG	1 cut(s)	740
BstYI	RGATCY	1 cut(s)	1021
BsuI	GTATCC	1 cut(s)	928
BsuRI	GGCC	4 cut(s)	242, 948, 1017, 1951
BtgI	CCRYGG	3 cut(s)	127, 1998, 2125
BtrI	CACGTC	1 cut(s)	223
BtsCI	GGATG	5 cut(s)	334, 628, 991, 1828, 1972
BtsI	GCAGTG	4 cut(s)	793, 1301, 1675, 2173
BtsIMutI	CAGTG	8 cut(s)	793, 913, 924, 1301, 1485, 1634, 1675, 2173
BveI	ACCTGC	3 cut(s)	1334, 1994, 2143
Cac8I	GCNNGC	9 cut(s)	44, 142, 327, 913, 1182, 1244, 2116, 2146, 2150
CfoI	GCGC	2 cut(s)	325, 376
Cfr10I	RCCGGY	1 cut(s)	317
Cfr13I	GGNCC	6 cut(s)	156, 254, 410, 855, 1881, 1950
Cfr42I	CCGCGG	1 cut(s)	130
CpoI	CGGWCCG	2 cut(s)	156, 254
CseI	GACGC	3 cut(s)	209, 311, 441
Csp6I	GTAC	4 cut(s)	482, 791, 1133, 1349
CspAI	ACCGGT	1 cut(s)	317
CspCI	CAANNNNNGTGG	2 cut(s)	2025, 2060
CspI	CGGWCCG	2 cut(s)	156, 254
CviQI	GTAC	4 cut(s)	482, 791, 1133, 1349
DdeI	CTNAG	3 cut(s)	272, 943, 1831
DpnI	GATC	9 cut(s)	76, 119, 149, 513, 1023, 1254, 1580, 1851, 1966
DpnII	GATC	9 cut(s)	74, 117, 147, 511, 1021, 1252, 1578, 1849, 1964
DraI	TTTAAA	1 cut(s)	1438
DraIII	CACNNNGTG	1 cut(s)	656
DriI	GACNNNNNGTC	1 cut(s)	853
EaeI	YGGCCR	1 cut(s)	1015
Eam1104I	CTCTTC	2 cut(s)	1731, 2180
Eam1105I	GACNNNNNGTC	1 cut(s)	853
EarI	CTCTTC	2 cut(s)	1731, 2180
EciI	GGCGGA	2 cut(s)	14, 220
Eco130I	CCWWGG	3 cut(s)	716, 888, 1998
Eco31I	GGTCTC	1 cut(s)	401
Eco32I	GATATC	2 cut(s)	500, 557
Eco47I	GGWCC	5 cut(s)	156, 254, 410, 855, 1881
Eco57I	CTGAAG	4 cut(s)	864, 965, 1334, 2161
Eco72I	CACGTG	1 cut(s)	862
Eco88I	CYCGRG	1 cut(s)	104
Eco91I	GGTNACC	1 cut(s)	1781
EcoO109I	RGGNCCY	1 cut(s)	1881
EcoO65I	GGTNACC	1 cut(s)	1781
EcoRI	GAATTC	2 cut(s)	174, 1540
EcoRII	CCWGG	3 cut(s)	337, 493, 1564
EcoRV	GATATC	2 cut(s)	500, 557
EcoT14I	CCWWGG	3 cut(s)	716, 888, 1998
EcoT22I	ATGCAT	1 cut(s)	1248
ErhI	CCWWGG	3 cut(s)	716, 888, 1998
FalI	AAGNNNNNCTT	6 cut(s)	627, 659, 1131, 1163, 1307, 1339
FaqI	GGGAC	5 cut(s)	446, 841, 1070, 1867, 1996
FauNDI	CATATG	1 cut(s)	1869
FokI	GGATG	5 cut(s)	321, 635, 978, 1835, 1959
FspBI	CTAG	3 cut(s)	1455, 1494, 1626
FspI	TGCGCA	2 cut(s)	324, 375
GlaI	GCGC	2 cut(s)	324, 375
GsuI	CTGGAG	4 cut(s)	716, 810, 1179, 1236
HaeIII	GGCC	4 cut(s)	242, 948, 1017, 1951
HgaI	GACGC	3 cut(s)	209, 311, 441
HhaI	GCGC	2 cut(s)	325, 376
Hin1I	GRCGYC	3 cut(s)	5, 201, 303
Hin6I	GCGC	2 cut(s)	323, 374
HinP1I	GCGC	2 cut(s)	323, 374
HindIII	AAGCTT	1 cut(s)	1268
HinfI	GANTC	7 cut(s)	86, 102, 112, 295, 1143, 1611, 2195
HphI	GGTGA	4 cut(s)	60, 391, 1185, 1793
Hpy166II	GTNNAC	4 cut(s)	791, 1349, 1840, 2125
Hpy188III	TCNNGA	9 cut(s)	106, 299, 509, 515, 620, 733, 1391, 1582, 1916
Hpy8I	GTNNAC	4 cut(s)	791, 1349, 1840, 2125
Hpy99I	CGWCG	5 cut(s)	188, 203, 239, 295, 305
HpyAV	CCTTC	5 cut(s)	1157, 1309, 1840, 2045, 2185
HpyCH4IV	ACGT	3 cut(s)	222, 655, 861
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 239, 881, 890, 1109, 1511, 2000, 2111
HpyF3I	CTNAG	3 cut(s)	272, 943, 1831
HpySE526I	ACGT	3 cut(s)	222, 655, 861
Hsp92I	GRCGYC	3 cut(s)	5, 201, 303
HspAI	GCGC	2 cut(s)	323, 374
KflI	GGGWCCC	1 cut(s)	1881
Kpn2I	TCCGGA	1 cut(s)	619
KpnI	GGTACC	1 cut(s)	485
KspI	CCGCGG	1 cut(s)	130
Kzo9I	GATC	9 cut(s)	74, 117, 147, 511, 1021, 1252, 1578, 1849, 1964
LmnI	GCTCC	8 cut(s)	222, 265, 647, 829, 835, 1189, 1716, 2089
Lsp1109I	GCAGC	7 cut(s)	485, 649, 652, 871, 1477, 1504, 1526
LweI	GCATC	3 cut(s)	428, 1000, 2023
MaeI	CTAG	3 cut(s)	1455, 1494, 1626
MaeII	ACGT	3 cut(s)	222, 655, 861
MaeIII	GTNAC	7 cut(s)	29, 506, 997, 1077, 1468, 1696, 1781
MalI	GATC	9 cut(s)	76, 119, 149, 513, 1023, 1254, 1580, 1851, 1966
MboI	GATC	9 cut(s)	74, 117, 147, 511, 1021, 1252, 1578, 1849, 1964
MflI	RGATCY	1 cut(s)	1021
MhlI	GDGCHC	1 cut(s)	652
MlsI	TGGCCA	1 cut(s)	1017
MluNI	TGGCCA	1 cut(s)	1017
MlyI	GAGTC	3 cut(s)	80, 96, 1620
MmeI	TCCRAC	2 cut(s)	82, 108
Mox20I	TGGCCA	1 cut(s)	1017
Mph1103I	ATGCAT	1 cut(s)	1248
MroI	TCCGGA	1 cut(s)	619
MroXI	GAANNNNTTC	3 cut(s)	639, 1062, 1560
MscI	TGGCCA	1 cut(s)	1017
MseI	TTAA	5 cut(s)	527, 566, 1437, 1724, 2184
MslI	CAYNNNNRTG	6 cut(s)	585, 756, 771, 965, 1620, 1868
Msp20I	TGGCCA	1 cut(s)	1017
MspA1I	CMGCKG	3 cut(s)	129, 1517, 2144
MspR9I	CCNGG	5 cut(s)	339, 495, 1114, 1566, 1980
MvaI	CCWGG	3 cut(s)	339, 495, 1566
MvnI	CGCG	2 cut(s)	129, 161
MwoI	GCNNNNNNNGC	8 cut(s)	11, 239, 881, 890, 1109, 1511, 2000, 2111
NciI	CCSGG	2 cut(s)	1114, 1980
NcoI	CCATGG	1 cut(s)	1998
NdeI	CATATG	1 cut(s)	1869
NdeII	GATC	9 cut(s)	74, 117, 147, 511, 1021, 1252, 1578, 1849, 1964
NlaIV	GGNNCC	7 cut(s)	261, 483, 857, 1504, 1882, 1883, 2106
NmeAIII	GCCGAG	1 cut(s)	247
NmuCI	GTSAC	4 cut(s)	506, 997, 1077, 1781
NsbI	TGCGCA	2 cut(s)	324, 375
NsiI	ATGCAT	1 cut(s)	1248
NspI	RCATGY	3 cut(s)	1184, 1246, 2096
PaeI	GCATGC	2 cut(s)	1184, 1246
PaeR7I	CTCGAG	1 cut(s)	104
PaqCI	CACCTGC	1 cut(s)	1334
PcsI	WCGNNNNNNNCGW	1 cut(s)	306
PdmI	GAANNNNTTC	3 cut(s)	639, 1062, 1560
PfeI	GAWTC	4 cut(s)	112, 295, 1143, 2195
PflMI	CCANNNNNTGG	1 cut(s)	581
PfoI	TCCNGGA	1 cut(s)	1564
PinAI	ACCGGT	1 cut(s)	317
PleI	GAGTC	3 cut(s)	80, 96, 1619
PmaCI	CACGTG	1 cut(s)	862
PmlI	CACGTG	1 cut(s)	862
PpsI	GAGTC	3 cut(s)	80, 96, 1619
Ppu21I	YACGTR	1 cut(s)	862
PpuMI	RGGWCCY	1 cut(s)	1881
PshBI	ATTAAT	1 cut(s)	527
Psp5II	RGGWCCY	1 cut(s)	1881
Psp6I	CCWGG	3 cut(s)	337, 493, 1564
PspCI	CACGTG	1 cut(s)	862
PspEI	GGTNACC	1 cut(s)	1781
PspGI	CCWGG	3 cut(s)	337, 493, 1564
PspN4I	GGNNCC	7 cut(s)	261, 483, 857, 1504, 1882, 1883, 2106
PspPI	GGNCC	6 cut(s)	156, 254, 410, 855, 1881, 1950
PspPPI	RGGWCCY	1 cut(s)	1881
PstI	CTGCAG	1 cut(s)	146
PsuI	RGATCY	1 cut(s)	1021
PvuII	CAGCTG	2 cut(s)	1517, 2144
RsaI	GTAC	4 cut(s)	483, 792, 1134, 1350
RsaNI	GTAC	4 cut(s)	482, 791, 1133, 1349
RseI	CAYNNNNRTG	6 cut(s)	585, 756, 771, 965, 1620, 1868
Rsr2I	CGGWCCG	2 cut(s)	156, 254
RsrII	CGGWCCG	2 cut(s)	156, 254
SacII	CCGCGG	1 cut(s)	130
SaqAI	TTAA	5 cut(s)	527, 566, 1437, 1724, 2184
Sau3AI	GATC	9 cut(s)	74, 117, 147, 511, 1021, 1252, 1578, 1849, 1964
Sau96I	GGNCC	6 cut(s)	156, 254, 410, 855, 1881, 1950
SbfI	CCTGCAGG	1 cut(s)	146
SchI	GAGTC	3 cut(s)	80, 96, 1620
ScrFI	CCNGG	5 cut(s)	339, 495, 1114, 1566, 1980
SdaI	CCTGCAGG	1 cut(s)	146
SduI	GDGCHC	1 cut(s)	652
SfaNI	GCATC	3 cut(s)	428, 1000, 2023
SfcI	CTRYAG	1 cut(s)	142
Sfr274I	CTCGAG	1 cut(s)	104
Sfr303I	CCGCGG	1 cut(s)	130
SgrAI	CRCCGGYG	1 cut(s)	317
SgrBI	CCGCGG	1 cut(s)	130
SinI	GGWCC	5 cut(s)	156, 254, 410, 855, 1881
SlaI	CTCGAG	1 cut(s)	104
SmiMI	CAYNNNNRTG	6 cut(s)	585, 756, 771, 965, 1620, 1868
SmlI	CTYRAG	2 cut(s)	104, 1643
SmoI	CTYRAG	2 cut(s)	104, 1643
SphI	GCATGC	2 cut(s)	1184, 1246
Sse8387I	CCTGCAGG	1 cut(s)	146
SspMI	CTAG	3 cut(s)	1455, 1494, 1626
StyD4I	CCNGG	5 cut(s)	337, 493, 1112, 1564, 1978
StyI	CCWWGG	3 cut(s)	716, 888, 1998
TaiI	ACGT	3 cut(s)	225, 658, 864
TaqI	TCGA	6 cut(s)	105, 293, 300, 1204, 1583, 1844
TaqII	GACCGA	2 cut(s)	242, 1016
TatI	WGTACW	1 cut(s)	790
TauI	GCSGC	6 cut(s)	94, 97, 132, 169, 308, 875
TfiI	GAWTC	4 cut(s)	112, 295, 1143, 2195
Tru1I	TTAA	5 cut(s)	527, 566, 1437, 1724, 2184
Tru9I	TTAA	5 cut(s)	527, 566, 1437, 1724, 2184
TscAI	CASTG	8 cut(s)	793, 913, 924, 1308, 1485, 1641, 1675, 2173
TseFI	GTSAC	4 cut(s)	506, 997, 1077, 1781
TseI	GCWGC	7 cut(s)	473, 662, 665, 884, 1490, 1514, 1517
Tsp45I	GTSAC	4 cut(s)	506, 997, 1077, 1781
TspGWI	ACGGA	1 cut(s)	41
TspRI	CASTG	8 cut(s)	793, 913, 924, 1308, 1485, 1641, 1675, 2173
Van91I	CCANNNNNTGG	1 cut(s)	581
VpaK11BI	GGWCC	5 cut(s)	156, 254, 410, 855, 1881
VspI	ATTAAT	1 cut(s)	527
XapI	RAATTY	4 cut(s)	174, 1038, 1394, 1540
XceI	RCATGY	3 cut(s)	1184, 1246, 2096
XcmI	CCANNNNNNNNNTGG	3 cut(s)	341, 433, 2005
XhoI	CTCGAG	1 cut(s)	104
XmnI	GAANNNNTTC	3 cut(s)	639, 1062, 1560
XspI	CTAG	3 cut(s)	1455, 1494, 1626
Zsp2I	ATGCAT	1 cut(s)	1248

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.