Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0011225

Physical Location & Seq

Genomic Coordinates Forward (+)

52236054 .. 52241188

Gene Structure 5135 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG322200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1749 bp

ATGGCGTCAGTGGCCACCGTCCTCCGCCGGAAGCTCTCCGGCAAGCCATTCCGTTTCCGATTACTACCAGTCCGATCATTCTCCGACTCGCCGCAGAGAATCGAGAAGATTCTGATAGCCAACCGGGGCGAAATTGCGTGCAGGATCATGAGGACCGCAAAGAGACTCGGAATTCAAACCGTCGCCGTTTTCAGCGACGCCGATAGACACTCGCTCCACGTCAAATCCGCCGACGAGGCCGTTCGTATCGGTCCGGCTCCGGCTCGGCTCAGCTACCTCAACGCCTCGTCGATTCTTGACGCCGCCGTTCGTACTGGTGCGCAGGCTATCCATCCTGGCTATGGTTTTCTGTCAGAGAGTTCCGACTTTGCTCAACTTTGTGAGGATAAGGGTCTTACCTTTATTGGGCCTCCAGCATCTGCCATCAGGGACATGGGTGATAAAAGTGCGTCAAAGAGAATAATGGGTGCAGCAGGGGTACCTCTTGTGCCTGGATATCATGGGAAAGACCAAGATATTGATCTAATGAAGATAGAAGCCGACAAGATTGGATATCCAATCTTAATAAAGCCAACTCATGGAGGTGGGGGAAAGGGTATGAGGATTGTGCAAAGTCCGGATGAGTTTGTTGAAGCCTTCCTTGGAGCACAACGTGAGGCTGCTGCTTCTTTTGGCGTAAACACAATTTTGCTGGAGAAATATATTACACAGCCAAGACACATAGAAGTCCAGATATTTGGGGATAAACACGGGAATGTTCTACATTTATATGAGAGAGATTGCAGCGTACAAAGAAGGCACCAGAAAATAATCGAAGAAGCCCCAGCTCCAAATGTTTCTGATGACTTCCGGTCTCACTTGGGTCAAGCGGCTGTATCTGCTGCCAAGGCAGTTAGCTATCACAGTGCCGGCACAGTGGAATTTATAGTCGATACAGTCTCAGGCCAATTTTATTTCATGGAGATGAATACCCGTCTTCAGGTTGAGCATCCTGTGACTGAGATGATTGTTGGCCAAGATCTTGTAGAGTGGCAAATTCGTGTAGCAAATGGAGAACACCTTCCCATTAGTCAGTCACAGGTCCCTTTGTCAGGTCATGCTTTTGAAGCCCGTATATATGCTGAAAATGTACCAAAAGGGTTCCTTCCTGCAACTGGAGTTCTTCATCACTATCAGCATGCTCCAGTTTCACCATCAGTTCGAGTTGAGACTGGAGTTGAACAAGGCGACAGTGTTAGCATGCATTATGATCCTATGATTGCCAAGCTTGTAGTATGGGGAGAAAATCGTGCAGTAGCACTGGTAAAACTGAAGGATTGCTTGACAAAGTTTCAGGTTGCAGGTGTACCAACCAACATCAGTTTTCTGTCAAAACTTGCCAACCATCGGGAATTTGAAAATGGGAATGTGGAAACTCATTTTATTGAACATTTTAAAGATGACCTGTTTGTCAACCCTAGTAATTTGGAAGTAATGAACACAGTGCTTGGTGCTGCTAGATTTGTTGCCAAACTGGCGGCTGCATGTCTCATTGAAAAGGAAAATTCTGTATTCAGAGAAAATCTTCCTGGAAGCAACAACACGATCTCCATATGGTATTCTTCTCCGCCTTTCAGAGTCAATCATTGTGCTAGGCATACAATGGAACTCGAGTGGGAGAATGAATATGATAGCAGTGGCTCAAGGTTATTGACCTATTCTATCACTTACAAATCTGATGGGAGCTATCTAATTGAGGTAGTCTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004485 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0005840 GO:0006082 GO:0006520 GO:0006551 GO:0006552 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009507 GO:0009536 GO:0009987 GO:0016054 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022626 GO:0031974 GO:0032991 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046395 GO:0046872 GO:0046914 GO:0050897 GO:0070013 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00280 ko01100 map00280 map01100

Pfam Domains

Protein Families

Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2

Protein Analysis

582

Amino Acids

64.21

Weight (kDa)

6.65

Isoelectric Point (pI)

40.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_carb_N	PF00289	34 - 142	2.2e-45	Biotin carboxylase, N-terminal domain
CPSase_L_D2	PF02786	147 - 355	1.2e-74	Carbamoyl-phosphate synthase L chain, ATP binding domain
Dala_Dala_lig_C	PF07478	174 - 324	2.8e-10	D-ala D-ala ligase C-terminus
Biotin_carb_C	PF02785	369 - 476	4.1e-36	Biotin carboxylase C-terminal domain
MCCA_BT	PF19331	489 - 580	4e-38	Methylcrotonoyl-CoA carboxylase subunit alpha BT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0011225)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03090 AT1G03090
fragaria_vesca	FvH4_2g23110 FvH4_2g23110 FvH4_2g23110 FvH4_2g23110
malus_domestica	MD11G1280500.v1.1
prunus_persica	Prupe.8G236400_v2.0.a1
pyrus_communis	pycom11g24820
rosa_chinensis	RchiOBHm_Chr6g0290331
rosa_laevigata	RLG00000012226
rosa_multiflora	Rmu_sc0013786.1_g000003
rosa_roxburghii	Rroxscaffold_7G00177150
rosa_rugosa	Rorug06G0211900
rosa_samantha	Rh6AG322200 Rh6BG329500 Rh6CG335400 Rh6DG322400
rosa_wichuraiana	Rw6G027820 Rw6G027920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1331
AasI	GACNNNNNNGTC	1 cut(s)	1448
Acc16I	TGCGCA	1 cut(s)	321
Acc36I	ACCTGC	1 cut(s)	1331
Acc65I	GGTACC	1 cut(s)	478
AccB1I	GGYRCC	2 cut(s)	478, 798
AccB7I	CCANNNNNTGG	1 cut(s)	578
AccIII	TCCGGA	1 cut(s)	616
AciI	CCGC	8 cut(s)	25, 92, 156, 228, 303, 869, 1517, 1607
AclWI	GGATC	2 cut(s)	152, 1244
AcoI	YGGCCR	2 cut(s)	12, 1012
AcsI	RAATTY	5 cut(s)	171, 920, 1035, 1391, 1543
AcuI	CTGAAG	2 cut(s)	962, 1331
AcyI	GRCGYC	3 cut(s)	5, 198, 300
AdeI	CACNNNGTG	1 cut(s)	653
AfaI	GTAC	5 cut(s)	313, 480, 789, 1131, 1347
AfiI	CCNNNNNNNGG	7 cut(s)	341, 405, 578, 979, 1091, 1154, 1386
AgsI	TTSAA	7 cut(s)	176, 632, 1106, 1220, 1397, 1427, 1535
AhdI	GACNNNNNGTC	1 cut(s)	850
AjiI	CACGTC	1 cut(s)	220
AjnI	CCWGG	3 cut(s)	334, 490, 1567
AjuI	GAANNNNNNNTTGG	2 cut(s)	624, 656
AluBI	AGCT	6 cut(s)	34, 273, 827, 897, 1267, 1725
AluI	AGCT	6 cut(s)	34, 273, 827, 897, 1267, 1725
Alw21I	GWGCWC	1 cut(s)	649
Alw26I	GTCTC	5 cut(s)	157, 858, 943, 1202, 1532
AlwI	GGATC	2 cut(s)	152, 1244
AlwNI	CAGNNNCTG	1 cut(s)	419
Ama87I	CYCGRG	1 cut(s)	1649
Aor13HI	TCCGGA	1 cut(s)	616
AoxI	GGCC	5 cut(s)	12, 237, 407, 943, 1012
ApeKI	GCWGC	7 cut(s)	470, 659, 662, 783, 881, 1493, 1520
ApoI	RAATTY	5 cut(s)	171, 920, 1035, 1391, 1543
Asp700I	GAANNNNTTC	2 cut(s)	1059, 1563
Asp718I	GGTACC	1 cut(s)	478
AspLEI	GCGC	1 cut(s)	322
AspS9I	GGNCC	4 cut(s)	153, 251, 407, 1081
AsuC2I	CCSGG	1 cut(s)	125
AsuHPI	GGTGA	2 cut(s)	449, 1182
AvaI	CYCGRG	1 cut(s)	1649
AvaII	GGWCC	3 cut(s)	153, 251, 1081
BalI	TGGCCA	2 cut(s)	14, 1014
BanI	GGYRCC	2 cut(s)	478, 798
BbsI	GAAGAC	1 cut(s)	968
Bbv12I	GWGCWC	1 cut(s)	649
BbvI	GCAGC	7 cut(s)	482, 646, 649, 795, 868, 1480, 1507
BccI	CCATC	5 cut(s)	339, 431, 1201, 1392, 1712
BceAI	ACGGC	3 cut(s)	170, 224, 290
BciT130I	CCWGG	3 cut(s)	336, 492, 1569
BcnI	CCSGG	1 cut(s)	125
BcoDI	GTCTC	5 cut(s)	157, 858, 943, 1202, 1532
BfaI	CTAG	3 cut(s)	1458, 1497, 1632
BfuAI	ACCTGC	1 cut(s)	1331
BglI	GCCNNNNNGGC	2 cut(s)	236, 1514
BglII	AGATCT	1 cut(s)	1018
BlpI	GCTNAGC	1 cut(s)	269
Bme1390I	CCNGG	4 cut(s)	125, 336, 492, 1569
Bme18I	GGWCC	3 cut(s)	153, 251, 1081
BmeRI	GACNNNNNGTC	1 cut(s)	850
BmeT110I	CYCGRG	1 cut(s)	1649
BmgBI	CACGTC	1 cut(s)	220
BmgT120I	GGNCC	4 cut(s)	153, 251, 407, 1081
BmiI	GGNNCC	5 cut(s)	258, 480, 800, 1083, 1142
BmrFI	CCNGG	4 cut(s)	125, 336, 492, 1569
BmsI	GCATC	2 cut(s)	425, 997
BpiI	GAAGAC	1 cut(s)	968
BpmI	CTGGAG	5 cut(s)	396, 713, 1167, 1176, 1233
Bpu1102I	GCTNAGC	1 cut(s)	269
BpuEI	CTTGAG	1 cut(s)	1666
BpuMI	CCSGG	1 cut(s)	125
BsaBI	GATNNNNATC	1 cut(s)	519
BsaHI	GRCGYC	3 cut(s)	5, 198, 300
BsaI	GGTCTC	1 cut(s)	858
BsaJI	CCNNGG	3 cut(s)	124, 640, 885
BsaWI	WCCGGW	2 cut(s)	616, 849
BsaXI	ACNNNNNCTCC	4 cut(s)	198, 228, 1571, 1601
Bsc4I	CCNNNNNNNGG	7 cut(s)	341, 405, 578, 979, 1091, 1154, 1386
Bse118I	RCCGGY	1 cut(s)	908
Bse1I	ACTGG	7 cut(s)	68, 319, 1159, 1184, 1216, 1305, 1518
Bse8I	GATNNNNATC	1 cut(s)	519
BseAI	TCCGGA	1 cut(s)	616
BseBI	CCWGG	3 cut(s)	336, 492, 1569
BseDI	CCNNGG	3 cut(s)	124, 640, 885
BseGI	GGATG	3 cut(s)	331, 625, 988
BseJI	GATNNNNATC	1 cut(s)	519
BseLI	CCNNNNNNNGG	7 cut(s)	341, 405, 578, 979, 1091, 1154, 1386
BseMII	CTCAG	3 cut(s)	283, 954, 990
BseNI	ACTGG	7 cut(s)	68, 319, 1159, 1184, 1216, 1305, 1518
BseXI	GCAGC	7 cut(s)	482, 646, 649, 795, 868, 1480, 1507
BseYI	CCCAGC	1 cut(s)	823
BsgI	GTGCAG	3 cut(s)	160, 489, 1311
BshFI	GGCC	5 cut(s)	14, 239, 409, 945, 1014
BshNI	GGYRCC	2 cut(s)	478, 798
BsiHKAI	GWGCWC	1 cut(s)	649
BsiHKCI	CYCGRG	1 cut(s)	1649
BsiSI	CCGG	8 cut(s)	28, 39, 124, 254, 260, 617, 850, 909
BslFI	GGGAC	2 cut(s)	443, 1067
BslI	CCNNNNNNNGG	7 cut(s)	341, 405, 578, 979, 1091, 1154, 1386
BsmAI	GTCTC	5 cut(s)	157, 858, 943, 1202, 1532
BsmFI	GGGAC	2 cut(s)	443, 1067
BsnI	GGCC	5 cut(s)	14, 239, 409, 945, 1014
Bso31I	GGTCTC	1 cut(s)	858
BsoBI	CYCGRG	1 cut(s)	1649
Bsp1286I	GDGCHC	1 cut(s)	649
Bsp13I	TCCGGA	1 cut(s)	616
Bsp143I	GATC	6 cut(s)	74, 144, 520, 1018, 1249, 1584
Bsp1720I	GCTNAGC	1 cut(s)	269
BspACI	CCGC	8 cut(s)	25, 92, 156, 228, 303, 869, 1517, 1607
BspANI	GGCC	5 cut(s)	14, 239, 409, 945, 1014
BspCNI	CTCAG	3 cut(s)	282, 953, 991
BspEI	TCCGGA	1 cut(s)	616
BspHI	TCATGA	1 cut(s)	147
BspLI	GGNNCC	5 cut(s)	258, 480, 800, 1083, 1142
BspMI	ACCTGC	1 cut(s)	1331
BspPI	GGATC	2 cut(s)	152, 1244
BspT107I	GGYRCC	2 cut(s)	478, 798
BspTNI	GGTCTC	1 cut(s)	858
BsrFI	RCCGGY	1 cut(s)	908
BsrI	ACTGG	7 cut(s)	68, 319, 1159, 1184, 1216, 1305, 1518
BssAI	RCCGGY	1 cut(s)	908
BssECI	CCNNGG	3 cut(s)	124, 640, 885
BssMI	GATC	6 cut(s)	74, 144, 520, 1018, 1249, 1584
BssNI	GRCGYC	3 cut(s)	5, 198, 300
BssT1I	CCWWGG	2 cut(s)	640, 885
Bst2UI	CCWGG	3 cut(s)	336, 492, 1569
Bst4CI	ACNGT	7 cut(s)	19, 181, 905, 916, 937, 1232, 1483
BstACI	GRCGYC	3 cut(s)	5, 198, 300
BstC8I	GCNNGC	6 cut(s)	44, 139, 324, 910, 1179, 1241
BstDEI	CTNAG	3 cut(s)	269, 940, 999
BstENI	CCTNNNNNAGG	1 cut(s)	1089
BstF5I	GGATG	3 cut(s)	331, 625, 988
BstHHI	GCGC	1 cut(s)	322
BstKTI	GATC	6 cut(s)	77, 147, 523, 1021, 1252, 1587
BstMAI	GTCTC	5 cut(s)	157, 858, 943, 1202, 1532
BstMBI	GATC	6 cut(s)	74, 144, 520, 1018, 1249, 1584
BstMWI	GCNNNNNNNGC	6 cut(s)	11, 236, 878, 887, 1106, 1514
BstNI	CCWGG	3 cut(s)	336, 492, 1569
BstNSI	RCATGY	3 cut(s)	1181, 1243, 1527
BstSCI	CCNGG	4 cut(s)	123, 334, 490, 1567
BstV1I	GCAGC	7 cut(s)	482, 646, 649, 795, 868, 1480, 1507
BstV2I	GAAGAC	1 cut(s)	968
BstX2I	RGATCY	1 cut(s)	1018
BstXI	CCANNNNNNTGG	1 cut(s)	737
BstYI	RGATCY	1 cut(s)	1018
BsuRI	GGCC	5 cut(s)	14, 239, 409, 945, 1014
BtrI	CACGTC	1 cut(s)	220
BtsCI	GGATG	3 cut(s)	331, 625, 988
BtsI	GCAGTG	1 cut(s)	1681
BtsIMutI	CAGTG	7 cut(s)	15, 910, 921, 1237, 1298, 1488, 1681
BveI	ACCTGC	1 cut(s)	1331
Cac8I	GCNNGC	6 cut(s)	44, 139, 324, 910, 1179, 1241
CaiI	CAGNNNCTG	1 cut(s)	419
CciI	TCATGA	1 cut(s)	147
CfoI	GCGC	1 cut(s)	322
Cfr10I	RCCGGY	1 cut(s)	908
Cfr13I	GGNCC	4 cut(s)	153, 251, 407, 1081
CpoI	CGGWCCG	1 cut(s)	251
CseI	GACGC	3 cut(s)	206, 308, 438
Csp6I	GTAC	5 cut(s)	312, 479, 788, 1130, 1346
CspI	CGGWCCG	1 cut(s)	251
CviAII	CATG	9 cut(s)	148, 433, 500, 578, 958, 1097, 1178, 1240, 1524
CviQI	GTAC	5 cut(s)	312, 479, 788, 1130, 1346
DdeI	CTNAG	3 cut(s)	269, 940, 999
DpnI	GATC	6 cut(s)	76, 146, 522, 1020, 1251, 1586
DpnII	GATC	6 cut(s)	74, 144, 520, 1018, 1249, 1584
DraI	TTTAAA	1 cut(s)	1435
DraIII	CACNNNGTG	1 cut(s)	653
DrdI	GACNNNNNNGTC	1 cut(s)	1448
DriI	GACNNNNNGTC	1 cut(s)	850
DseDI	GACNNNNNNGTC	1 cut(s)	1448
EaeI	YGGCCR	2 cut(s)	12, 1012
Eam1105I	GACNNNNNGTC	1 cut(s)	850
EciI	GGCGGA	3 cut(s)	14, 217, 1596
Eco130I	CCWWGG	2 cut(s)	640, 885
Eco31I	GGTCTC	1 cut(s)	858
Eco32I	GATATC	2 cut(s)	497, 554
Eco47I	GGWCC	3 cut(s)	153, 251, 1081
Eco57I	CTGAAG	2 cut(s)	962, 1331
Eco88I	CYCGRG	1 cut(s)	1649
EcoNI	CCTNNNNNAGG	1 cut(s)	1089
EcoO109I	RGGNCCY	1 cut(s)	1081
EcoRI	GAATTC	1 cut(s)	171
EcoRII	CCWGG	3 cut(s)	334, 490, 1567
EcoRV	GATATC	2 cut(s)	497, 554
EcoT14I	CCWWGG	2 cut(s)	640, 885
EcoT22I	ATGCAT	1 cut(s)	1245
ErhI	CCWWGG	2 cut(s)	640, 885
FaeI	CATG	9 cut(s)	151, 436, 503, 581, 961, 1100, 1181, 1243, 1527
FalI	AAGNNNNNCTT	6 cut(s)	624, 656, 1128, 1160, 1304, 1336
FaqI	GGGAC	2 cut(s)	443, 1067
FatI	CATG	9 cut(s)	147, 432, 499, 577, 957, 1096, 1177, 1239, 1523
FauNDI	CATATG	1 cut(s)	1592
FokI	GGATG	3 cut(s)	318, 632, 975
FspBI	CTAG	3 cut(s)	1458, 1497, 1632
FspI	TGCGCA	1 cut(s)	321
GlaI	GCGC	1 cut(s)	321
GsaI	CCCAGC	1 cut(s)	827
GsuI	CTGGAG	5 cut(s)	396, 713, 1167, 1176, 1233
HaeIII	GGCC	5 cut(s)	14, 239, 409, 945, 1014
HapII	CCGG	8 cut(s)	28, 39, 124, 254, 260, 617, 850, 909
HgaI	GACGC	3 cut(s)	206, 308, 438
HhaI	GCGC	1 cut(s)	322
Hin1I	GRCGYC	3 cut(s)	5, 198, 300
Hin1II	CATG	9 cut(s)	151, 436, 503, 581, 961, 1100, 1181, 1243, 1527
Hin6I	GCGC	1 cut(s)	320
HinP1I	GCGC	1 cut(s)	320
HincII	GTYRAC	1 cut(s)	1453
HindII	GTYRAC	1 cut(s)	1453
HindIII	AAGCTT	1 cut(s)	1265
HinfI	GANTC	6 cut(s)	86, 99, 109, 165, 292, 1617
HpaII	CCGG	8 cut(s)	28, 39, 124, 254, 260, 617, 850, 909
HphI	GGTGA	2 cut(s)	449, 1182
Hpy166II	GTNNAC	3 cut(s)	679, 1346, 1453
Hpy188III	TCNNGA	6 cut(s)	103, 148, 296, 617, 730, 1388
Hpy8I	GTNNAC	3 cut(s)	679, 1346, 1453
Hpy99I	CGWCG	4 cut(s)	185, 200, 236, 292
HpyAV	CCTTC	5 cut(s)	646, 789, 1070, 1154, 1306
HpyCH4III	ACNGT	7 cut(s)	19, 181, 905, 916, 937, 1232, 1483
HpyCH4IV	ACGT	2 cut(s)	219, 652
HpyCH4V	TGCA	9 cut(s)	141, 470, 610, 783, 1151, 1243, 1292, 1340, 1523
HpyF10VI	GCNNNNNNNGC	6 cut(s)	11, 236, 878, 887, 1106, 1514
HpyF3I	CTNAG	3 cut(s)	269, 940, 999
HpySE526I	ACGT	2 cut(s)	219, 652
Hsp92I	GRCGYC	3 cut(s)	5, 198, 300
Hsp92II	CATG	9 cut(s)	151, 436, 503, 581, 961, 1100, 1181, 1243, 1527
HspAI	GCGC	1 cut(s)	320
Kpn2I	TCCGGA	1 cut(s)	616
KpnI	GGTACC	1 cut(s)	482
KroI	GCCGGC	1 cut(s)	908
KroNI	GCCGGC	1 cut(s)	910
Kzo9I	GATC	6 cut(s)	74, 144, 520, 1018, 1249, 1584
LmnI	GCTCC	6 cut(s)	219, 262, 644, 832, 1186, 1722
Lsp1109I	GCAGC	7 cut(s)	482, 646, 649, 795, 868, 1480, 1507
LweI	GCATC	2 cut(s)	425, 997
MaeI	CTAG	3 cut(s)	1458, 1497, 1632
MaeII	ACGT	2 cut(s)	219, 652
MaeIII	GTNAC	2 cut(s)	994, 1074
MalI	GATC	6 cut(s)	76, 146, 522, 1020, 1251, 1586
MboI	GATC	6 cut(s)	74, 144, 520, 1018, 1249, 1584
MboII	GAAGA	7 cut(s)	118, 541, 827, 968, 1154, 1556, 1593
MflI	RGATCY	1 cut(s)	1018
MhlI	GDGCHC	1 cut(s)	649
MlsI	TGGCCA	2 cut(s)	14, 1014
MluNI	TGGCCA	2 cut(s)	14, 1014
MlyI	GAGTC	3 cut(s)	80, 159, 1626
MmeI	TCCRAC	2 cut(s)	108, 387
Mox20I	TGGCCA	2 cut(s)	14, 1014
Mph1103I	ATGCAT	1 cut(s)	1245
MroI	TCCGGA	1 cut(s)	616
MroNI	GCCGGC	1 cut(s)	908
MroXI	GAANNNNTTC	2 cut(s)	1059, 1563
MscI	TGGCCA	2 cut(s)	14, 1014
MseI	TTAA	2 cut(s)	563, 1434
MslI	CAYNNNNRTG	4 cut(s)	582, 753, 768, 962
Msp20I	TGGCCA	2 cut(s)	14, 1014
MspI	CCGG	8 cut(s)	28, 39, 124, 254, 260, 617, 850, 909
MspR9I	CCNGG	4 cut(s)	125, 336, 492, 1569
MvaI	CCWGG	3 cut(s)	336, 492, 1569
MwoI	GCNNNNNNNGC	6 cut(s)	11, 236, 878, 887, 1106, 1514
NaeI	GCCGGC	1 cut(s)	910
NciI	CCSGG	1 cut(s)	125
NdeI	CATATG	1 cut(s)	1592
NdeII	GATC	6 cut(s)	74, 144, 520, 1018, 1249, 1584
NgoMIV	GCCGGC	1 cut(s)	908
NlaIII	CATG	9 cut(s)	151, 436, 503, 581, 961, 1100, 1181, 1243, 1527
NlaIV	GGNNCC	5 cut(s)	258, 480, 800, 1083, 1142
NmeAIII	GCCGAG	1 cut(s)	244
NmuCI	GTSAC	2 cut(s)	994, 1074
NsbI	TGCGCA	1 cut(s)	321
NsiI	ATGCAT	1 cut(s)	1245
NspI	RCATGY	3 cut(s)	1181, 1243, 1527
PaeI	GCATGC	2 cut(s)	1181, 1243
PaeR7I	CTCGAG	1 cut(s)	1649
PagI	TCATGA	1 cut(s)	147
PaqCI	CACCTGC	1 cut(s)	1331
PdiI	GCCGGC	1 cut(s)	910
PdmI	GAANNNNTTC	2 cut(s)	1059, 1563
PfeI	GAWTC	3 cut(s)	99, 109, 292
PflMI	CCANNNNNTGG	1 cut(s)	578
PfoI	TCCNGGA	1 cut(s)	1567
PleI	GAGTC	3 cut(s)	80, 159, 1625
PpsI	GAGTC	3 cut(s)	80, 159, 1625
PpuMI	RGGWCCY	1 cut(s)	1081
Psp5II	RGGWCCY	1 cut(s)	1081
Psp6I	CCWGG	3 cut(s)	334, 490, 1567
PspFI	CCCAGC	1 cut(s)	823
PspGI	CCWGG	3 cut(s)	334, 490, 1567
PspN4I	GGNNCC	5 cut(s)	258, 480, 800, 1083, 1142
PspPI	GGNCC	4 cut(s)	153, 251, 407, 1081
PspPPI	RGGWCCY	1 cut(s)	1081
PspXI	VCTCGAGB	1 cut(s)	1649
PstNI	CAGNNNCTG	1 cut(s)	419
PsuI	RGATCY	1 cut(s)	1018
RsaI	GTAC	5 cut(s)	313, 480, 789, 1131, 1347
RsaNI	GTAC	5 cut(s)	312, 479, 788, 1130, 1346
RseI	CAYNNNNRTG	4 cut(s)	582, 753, 768, 962
Rsr2I	CGGWCCG	1 cut(s)	251
RsrII	CGGWCCG	1 cut(s)	251
SaqAI	TTAA	2 cut(s)	563, 1434
Sau3AI	GATC	6 cut(s)	74, 144, 520, 1018, 1249, 1584
Sau96I	GGNCC	4 cut(s)	153, 251, 407, 1081
SchI	GAGTC	3 cut(s)	80, 159, 1626
ScrFI	CCNGG	4 cut(s)	125, 336, 492, 1569
SduI	GDGCHC	1 cut(s)	649
SfaNI	GCATC	2 cut(s)	425, 997
Sfr274I	CTCGAG	1 cut(s)	1649
SinI	GGWCC	3 cut(s)	153, 251, 1081
SlaI	CTCGAG	1 cut(s)	1649
SmiMI	CAYNNNNRTG	4 cut(s)	582, 753, 768, 962
SmlI	CTYRAG	2 cut(s)	1649, 1681
SmoI	CTYRAG	2 cut(s)	1649, 1681
SphI	GCATGC	2 cut(s)	1181, 1243
SsiI	CCGC	8 cut(s)	25, 92, 156, 228, 303, 869, 1517, 1607
SspMI	CTAG	3 cut(s)	1458, 1497, 1632
StyD4I	CCNGG	4 cut(s)	123, 334, 490, 1567
StyI	CCWWGG	2 cut(s)	640, 885
TaaI	ACNGT	7 cut(s)	19, 181, 905, 916, 937, 1232, 1483
TaiI	ACGT	2 cut(s)	222, 655
TaqI	TCGA	6 cut(s)	102, 290, 813, 930, 1201, 1650
TaqII	GACCGA	1 cut(s)	239
TauI	GCSGC	4 cut(s)	94, 305, 872, 1520
TfiI	GAWTC	3 cut(s)	99, 109, 292
Tru1I	TTAA	2 cut(s)	563, 1434
Tru9I	TTAA	2 cut(s)	563, 1434
TscAI	CASTG	7 cut(s)	15, 910, 921, 1237, 1305, 1488, 1681
TseFI	GTSAC	2 cut(s)	994, 1074
TseI	GCWGC	7 cut(s)	470, 659, 662, 783, 881, 1493, 1520
Tsp45I	GTSAC	2 cut(s)	994, 1074
TspDTI	ATGAA	6 cut(s)	542, 946, 980, 1154, 1490, 1677
TspGWI	ACGGA	1 cut(s)	41
TspRI	CASTG	7 cut(s)	15, 910, 921, 1237, 1305, 1488, 1681
Van91I	CCANNNNNTGG	1 cut(s)	578
VpaK11BI	GGWCC	3 cut(s)	153, 251, 1081
XagI	CCTNNNNNAGG	1 cut(s)	1089
XapI	RAATTY	5 cut(s)	171, 920, 1035, 1391, 1543
XceI	RCATGY	3 cut(s)	1181, 1243, 1527
XcmI	CCANNNNNNNNNTGG	2 cut(s)	338, 430
XhoI	CTCGAG	1 cut(s)	1649
XmnI	GAANNNNTTC	2 cut(s)	1059, 1563
XspI	CTAG	3 cut(s)	1458, 1497, 1632
Zsp2I	ATGCAT	1 cut(s)	1245

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.