Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0011225

Physical Location & Seq

Genomic Coordinates Forward (+)

39827569 .. 39833518

Gene Structure 5950 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1280500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2202 bp

ATGCCGTCGTTCGCCACCGTCCTCCGCCGGAAGCTCTCCGGCAAAGCCTTCCATTTCCGGATAGTAACAGTAAGAGAATTCTCGGCCTCCGAGCCGCGGAGGATAGAGAAGATTCTGATAGCGAACAGAGGCGAAATAGCCTGCAGGATCATGCGGACAGCCCAGCGACTCGGGATTCAAACCGTCGCCGTTTACAGCGACGCCGATAGGTATTCGCTTCATGTCAAATCGGCCGACGAGGCCGTGCATATTGGACCTCCACCGGCACGGCTCAGCTACCTCAACGCCTCCTCGATTCTCGACGCCGCCGTTCGTACTGGCGCTCAGGCTATTCATCCTGGCTATGGCTTTCTGTCGGAAAGTGCTGACTTTGCTCAACTTTGTGAAGATAAAGGTCTTACATTTATCGGGCCTCCGGCATCTGCCATTCGCGACATGGGTGATAAAAGTGCTTCAAAGAGAATAATGGGTGCGGCAGGGGTGCCGCTTGTGCCTGGATATCATGGTAACGATCAAGATATTGATCTAATGAAGTTAGAAGCTGACAAGATTGGATATCCAATCTTAATAAAGCCAACTCATGGAGGTGGAGGAAAGGGTATGAGGATTGTGCAGAGTCCTGATGAATTTGTCGAAGCTTTTTTGGGAGCACAACGTGAGGCTGCTGCTTCTTTTGGCGTTAGCACAATATTGTTGGAGAAGTATATTACACGGCCAAGGCACATAGAAGTTCAGATCTTTGGGGACAAACATGGGAATGTTCTACATTTATATGAAAGAGATTGCAGCGTCCAGAGAAGACACCAGAAAATAATTGAAGAAGCCCCAGCTCCAAATGTTTCTAATGACTTTCGATCTCACTTGGGTCAAGCTGCTGTATCTGCTGCCAAGGCAGTTGGTTATCACAATGCTGGCACTGTGGAGTTTATAGTTGATACAGTCTCAGGCCAATTTTATTTCATGGAGATGAACACTCGACTTCAGGTTGAGCATCCTGTGACTGAGATGATTGTCGGCCAAGATCTTGTAGAGTGGCAAATTCGTGTTGCAAACGGAGAACATCTTCCTATTAGTCAGTCACAGATCCCCTTATCAGGTCATGCTTTCGAAGCCCGAATATATGCTGAAAATGTACCGAAAGGATTCCTTCCAGCAACTGGAGTCCTTCATCACTATCATCATGTTCCAGTCTCACCAACAGTTAGAGTTGAGACTGGAGTTGAACGTGGAGACACTGTTAGCATGCATTATGATCCTATGATTGCTAAGCTTGTAGTATGGGGAGAAAATCGAACTGCAGCATTGGTGAAATTGAAGGATTGTTTGTCAAAGTTTCAGGTTGCAGGTTTACCAACCAACATCAATTTTCTGCTAAAACTTGCTAGCCATCAGGCATTTGAAAATGGTGATGTGGAAACCCATTTTATTGAACGCTTTAAAGATGACCTCTTTGTCGACCCTAGTAATTCACTACTGGTGGACAAAGTGGCTGGTGCTGCTAGATTTGGTGCTACACTTGCAGCTGCGTGTCTCATTGAAAAGGAAAATTCTGAATTTAGAGAAAATCTTCCTGGCAGCAAAGGCATAAACTCCATATGGTATTCTTCTCCCCCCTTCAGAGTCCATCATTGTGCTAGACATACTATGGAGCTTGAGTGGGAAAATGAATACGATAGCAGTGGCTCAAAGTTACTGAAGATTTCAACCACTTATAAAGAAGATGGGAGCTATCTGATTGAGGCAGAAGAAGACAGTTCCCAATGTGTGGAGGTCAAAGTAACTTGCATAGGCAACCACGATTTCCGAGTTGAAGCTGATGGTGTAATCATGGATGTTTGCTTAGCTGTTTACTCCAAGGACCAAACCCAGCTCATTCATATATGGCATGGGTCACATCATCATCATTTCAAGCAGAAAATTGGTCTTGAATTGTCTGATGATGATGATCTCGAACATAAGCCCAGATTTGAGAAATCGTCATACCCTCAGGGCAGTGTTGTTGCGCCCATGGCTGGGTTGGTGGTCAAGCTTCTGGTCAAGGATGGGACAAAGGTGGAAGGAGGACAGCCCATACTAGTTTTAGAGGCAATGAAGATGGAGCATGTTGTAAAGGCACCATCAGCGGGCTATGTCCACGGGCTTCATCTAGCAGCTGGCCAGCAGGTTTCTGATGGTAGTGTTCTCTTCAGTATCAAGGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004485 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0005840 GO:0006082 GO:0006520 GO:0006551 GO:0006552 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009507 GO:0009536 GO:0009987 GO:0016054 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022626 GO:0031974 GO:0032991 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046395 GO:0046872 GO:0046914 GO:0050897 GO:0070013 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00280 ko01100 map00280 map01100

Pfam Domains

Protein Families

Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2

Protein Analysis

734

Amino Acids

80.73

Weight (kDa)

6.23

Isoelectric Point (pI)

33.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_carb_N	PF00289	35 - 143	2.9e-45	Biotin carboxylase, N-terminal domain
CPSase_L_D2	PF02786	148 - 356	2e-74	Carbamoyl-phosphate synthase L chain, ATP binding domain
Dala_Dala_lig_C	PF07478	175 - 325	5.5e-10	D-ala D-ala ligase C-terminus
Biotin_carb_C	PF02785	370 - 478	1.8e-35	Biotin carboxylase C-terminal domain
MCCA_BT	PF19331	489 - 653	4.9e-79	Methylcrotonoyl-CoA carboxylase subunit alpha BT domain
Biotin_lipoyl	PF00364	666 - 731	9.7e-17	Biotin-requiring enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011225)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03090 AT1G03090
fragaria_vesca	FvH4_2g23110 FvH4_2g23110 FvH4_2g23110 FvH4_2g23110
malus_domestica	MD11G1280500.v1.1
prunus_persica	Prupe.8G236400_v2.0.a1
pyrus_communis	pycom11g24820
rosa_chinensis	RchiOBHm_Chr6g0290331
rosa_laevigata	RLG00000012226
rosa_multiflora	Rmu_sc0013786.1_g000003
rosa_roxburghii	Rroxscaffold_7G00177150
rosa_rugosa	Rorug06G0211900
rosa_samantha	Rh6AG322200 Rh6BG329500 Rh6CG335400 Rh6DG322400
rosa_wichuraiana	Rw6G027820 Rw6G027920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1713
AasI	GACNNNNNNGTC	1 cut(s)	1451
Acc36I	ACCTGC	2 cut(s)	1334, 2152
AccB1I	GGYRCC	2 cut(s)	481, 2113
AccB7I	CCANNNNNTGG	3 cut(s)	581, 1157, 1765
AccI	GTMKAC	1 cut(s)	1455
AccII	CGCG	2 cut(s)	97, 432
AccIII	TCCGGA	1 cut(s)	57
AciI	CCGC	8 cut(s)	25, 95, 97, 154, 306, 473, 485, 2123
AclWI	GGATC	3 cut(s)	155, 1078, 1247
AcoI	YGGCCR	4 cut(s)	231, 713, 1015, 2155
AcsI	RAATTY	5 cut(s)	77, 626, 1038, 1546, 1553
AcuI	CTGAAG	4 cut(s)	965, 1600, 1715, 2170
AcyI	GRCGYC	2 cut(s)	201, 303
AdeI	CACNNNGTG	1 cut(s)	656
AfaI	GTAC	2 cut(s)	316, 1134
AfiI	CCNNNNNNNGG	9 cut(s)	96, 344, 581, 1094, 1157, 1765, 2012, 2013, 2123
AhlI	ACTAGT	1 cut(s)	2074
AjnI	CCWGG	3 cut(s)	337, 493, 1570
Alw21I	GWGCWC	1 cut(s)	652
Alw26I	GTCTC	5 cut(s)	946, 1195, 1205, 1224, 1535
AlwI	GGATC	3 cut(s)	155, 1078, 1247
AlwNI	CAGNNNCTG	1 cut(s)	1157
Ama87I	CYCGRG	1 cut(s)	170
Aor13HI	TCCGGA	1 cut(s)	57
AoxI	GGCC	8 cut(s)	84, 231, 240, 410, 713, 946, 1015, 2155
ApoI	RAATTY	5 cut(s)	77, 626, 1038, 1546, 1553
Asp700I	GAANNNNTTC	3 cut(s)	1062, 1142, 1566
AspLEI	GCGC	2 cut(s)	323, 2005
AspS9I	GGNCC	3 cut(s)	254, 410, 1858
AsuHPI	GGTGA	4 cut(s)	452, 1185, 1318, 1418
AsuII	TTCGAA	1 cut(s)	1107
AsuNHI	GCTAGC	1 cut(s)	1382
AvaI	CYCGRG	1 cut(s)	170
AvaII	GGWCC	2 cut(s)	254, 1858
AxyI	CCTNAGG	1 cut(s)	1986
BalI	TGGCCA	1 cut(s)	2157
BanI	GGYRCC	2 cut(s)	481, 2113
BbsI	GAAGAC	2 cut(s)	805, 1755
Bbv12I	GWGCWC	1 cut(s)	652
BccI	CCATC	8 cut(s)	1395, 1632, 1715, 1811, 2036, 2089, 2125, 2165
BceAI	ACGGC	5 cut(s)	173, 227, 284, 293, 728
BciT130I	CCWGG	3 cut(s)	339, 495, 1572
BcoDI	GTCTC	5 cut(s)	946, 1195, 1205, 1224, 1535
BcuI	ACTAGT	1 cut(s)	2074
BfaI	CTAG	6 cut(s)	1383, 1461, 1500, 1635, 2075, 2147
BfmI	CTRYAG	2 cut(s)	142, 1296
BfoI	RGCGCY	1 cut(s)	324
BfuAI	ACCTGC	2 cut(s)	1334, 2152
BglI	GCCNNNNNGGC	1 cut(s)	239
BglII	AGATCT	2 cut(s)	735, 1021
BlpI	GCTNAGC	3 cut(s)	272, 1266, 1840
Bme1390I	CCNGG	3 cut(s)	339, 495, 1572
Bme18I	GGWCC	2 cut(s)	254, 1858
BmeT110I	CYCGRG	1 cut(s)	170
BmgT120I	GGNCC	3 cut(s)	254, 410, 1858
BmiI	GGNNCC	2 cut(s)	483, 2115
BmrFI	CCNGG	3 cut(s)	339, 495, 1572
BmsI	GCATC	2 cut(s)	428, 1000
BmtI	GCTAGC	1 cut(s)	1386
BpiI	GAAGAC	2 cut(s)	805, 1755
BpmI	CTGGAG	2 cut(s)	1179, 1236
Bpu10I	CCTNAGC	1 cut(s)	324
Bpu1102I	GCTNAGC	3 cut(s)	272, 1266, 1840
Bpu14I	TTCGAA	1 cut(s)	1107
BpuEI	CTTGAG	1 cut(s)	1673
BsaBI	GATNNNNATC	2 cut(s)	522, 1944
BsaHI	GRCGYC	2 cut(s)	201, 303
BsaJI	CCNNGG	6 cut(s)	95, 716, 888, 1854, 2007, 2134
BsaWI	WCCGGW	1 cut(s)	57
BsaXI	ACNNNNNCTCC	4 cut(s)	1221, 1251, 1640, 1670
Bsc4I	CCNNNNNNNGG	9 cut(s)	96, 344, 581, 1094, 1157, 1765, 2012, 2013, 2123
Bse118I	RCCGGY	1 cut(s)	262
Bse1I	ACTGG	5 cut(s)	322, 1162, 1187, 1219, 1479
Bse21I	CCTNAGG	1 cut(s)	1986
Bse3DI	GCAATG	1 cut(s)	2094
Bse8I	GATNNNNATC	2 cut(s)	522, 1944
BseAI	TCCGGA	1 cut(s)	57
BseBI	CCWGG	3 cut(s)	339, 495, 1572
BseDI	CCNNGG	6 cut(s)	95, 716, 888, 1854, 2007, 2134
BseGI	GGATG	4 cut(s)	334, 991, 1837, 2047
BseJI	GATNNNNATC	2 cut(s)	522, 1944
BseLI	CCNNNNNNNGG	9 cut(s)	96, 344, 581, 1094, 1157, 1765, 2012, 2013, 2123
BseMI	GCAATG	1 cut(s)	2094
BseMII	CTCAG	5 cut(s)	286, 338, 957, 993, 2000
BseNI	ACTGG	5 cut(s)	322, 1162, 1187, 1219, 1479
BseRI	GAGGAG	1 cut(s)	280
BseX3I	CGGCCG	1 cut(s)	231
BseYI	CCCAGC	4 cut(s)	162, 826, 1866, 2012
BsgI	GTGCAG	1 cut(s)	632
Bsh1236I	CGCG	2 cut(s)	97, 432
Bsh1285I	CGRYCG	1 cut(s)	234
BshFI	GGCC	8 cut(s)	86, 233, 242, 412, 715, 948, 1017, 2157
BshNI	GGYRCC	2 cut(s)	481, 2113
BsiEI	CGRYCG	1 cut(s)	234
BsiHKAI	GWGCWC	1 cut(s)	652
BsiHKCI	CYCGRG	1 cut(s)	170
BsiSI	CCGG	5 cut(s)	28, 39, 58, 263, 416
BslFI	GGGAC	2 cut(s)	758, 2059
BslI	CCNNNNNNNGG	9 cut(s)	96, 344, 581, 1094, 1157, 1765, 2012, 2013, 2123
BsmAI	GTCTC	5 cut(s)	946, 1195, 1205, 1224, 1535
BsmFI	GGGAC	2 cut(s)	758, 2059
BsnI	GGCC	8 cut(s)	86, 233, 242, 412, 715, 948, 1017, 2157
BsoBI	CYCGRG	1 cut(s)	170
Bsp119I	TTCGAA	1 cut(s)	1107
Bsp1286I	GDGCHC	1 cut(s)	652
Bsp13I	TCCGGA	1 cut(s)	57
Bsp143I	GATC	9 cut(s)	147, 511, 523, 735, 854, 1021, 1083, 1252, 1945
Bsp1720I	GCTNAGC	3 cut(s)	272, 1266, 1840
Bsp19I	CCATGG	1 cut(s)	2007
Bsp68I	TCGCGA	1 cut(s)	432
BspACI	CCGC	8 cut(s)	25, 95, 97, 154, 306, 473, 485, 2123
BspANI	GGCC	8 cut(s)	86, 233, 242, 412, 715, 948, 1017, 2157
BspCNI	CTCAG	5 cut(s)	285, 337, 956, 994, 1999
BspEI	TCCGGA	1 cut(s)	57
BspFNI	CGCG	2 cut(s)	97, 432
BspLI	GGNNCC	2 cut(s)	483, 2115
BspMAI	CTGCAG	2 cut(s)	146, 1300
BspMI	ACCTGC	2 cut(s)	1334, 2152
BspOI	GCTAGC	1 cut(s)	1386
BspPI	GGATC	3 cut(s)	155, 1078, 1247
BspT104I	TTCGAA	1 cut(s)	1107
BspT107I	GGYRCC	2 cut(s)	481, 2113
BsrDI	GCAATG	1 cut(s)	2094
BsrFI	RCCGGY	1 cut(s)	262
BsrI	ACTGG	5 cut(s)	322, 1162, 1187, 1219, 1479
BssAI	RCCGGY	1 cut(s)	262
BssECI	CCNNGG	6 cut(s)	95, 716, 888, 1854, 2007, 2134
BssMI	GATC	9 cut(s)	147, 511, 523, 735, 854, 1021, 1083, 1252, 1945
BssNI	GRCGYC	2 cut(s)	201, 303
BssT1I	CCWWGG	4 cut(s)	716, 888, 1854, 2007
Bst2UI	CCWGG	3 cut(s)	339, 495, 1572
Bst4CI	ACNGT	8 cut(s)	19, 70, 184, 919, 940, 1201, 1237, 1754
Bst6I	CTCTTC	1 cut(s)	2189
BstACI	GRCGYC	2 cut(s)	201, 303
BstBI	TTCGAA	1 cut(s)	1107
BstC8I	GCNNGC	7 cut(s)	142, 913, 1244, 1384, 2125, 2155, 2159
BstDEI	CTNAG	7 cut(s)	272, 324, 943, 1002, 1266, 1840, 1986
BstDSI	CCRYGG	3 cut(s)	95, 2007, 2134
BstF5I	GGATG	4 cut(s)	334, 991, 1837, 2047
BstFNI	CGCG	2 cut(s)	97, 432
BstH2I	RGCGCY	1 cut(s)	324
BstHHI	GCGC	2 cut(s)	323, 2005
BstKTI	GATC	9 cut(s)	150, 514, 526, 738, 857, 1024, 1086, 1255, 1948
BstMAI	GTCTC	5 cut(s)	946, 1195, 1205, 1224, 1535
BstMBI	GATC	9 cut(s)	147, 511, 523, 735, 854, 1021, 1083, 1252, 1945
BstMCI	CGRYCG	1 cut(s)	234
BstNI	CCWGG	3 cut(s)	339, 495, 1572
BstNSI	RCATGY	2 cut(s)	1246, 2105
BstSCI	CCNGG	3 cut(s)	337, 493, 1570
BstSFI	CTRYAG	2 cut(s)	142, 1296
BstUI	CGCG	2 cut(s)	97, 432
BstV2I	GAAGAC	2 cut(s)	805, 1755
BstX2I	RGATCY	3 cut(s)	735, 1021, 1083
BstYI	RGATCY	3 cut(s)	735, 1021, 1083
BstZI	CGGCCG	1 cut(s)	231
Bsu36I	CCTNAGG	1 cut(s)	1986
BsuRI	GGCC	8 cut(s)	86, 233, 242, 412, 715, 948, 1017, 2157
BtgI	CCRYGG	3 cut(s)	95, 2007, 2134
BtsCI	GGATG	4 cut(s)	334, 991, 1837, 2047
BtsI	GCAGTG	2 cut(s)	1684, 1999
BtsIMutI	CAGTG	4 cut(s)	915, 1233, 1684, 1999
BtuMI	TCGCGA	1 cut(s)	432
BveI	ACCTGC	2 cut(s)	1334, 2152
Cac8I	GCNNGC	7 cut(s)	142, 913, 1244, 1384, 2125, 2155, 2159
CaiI	CAGNNNCTG	1 cut(s)	1157
CfoI	GCGC	2 cut(s)	323, 2005
Cfr10I	RCCGGY	1 cut(s)	262
Cfr13I	GGNCC	3 cut(s)	254, 410, 1858
Cfr42I	CCGCGG	1 cut(s)	98
CseI	GACGC	3 cut(s)	209, 311, 778
Csp6I	GTAC	2 cut(s)	315, 1133
CviQI	GTAC	2 cut(s)	315, 1133
DdeI	CTNAG	7 cut(s)	272, 324, 943, 1002, 1266, 1840, 1986
DpnI	GATC	9 cut(s)	149, 513, 525, 737, 856, 1023, 1085, 1254, 1947
DpnII	GATC	9 cut(s)	147, 511, 523, 735, 854, 1021, 1083, 1252, 1945
DraI	TTTAAA	1 cut(s)	1438
DraIII	CACNNNGTG	1 cut(s)	656
DrdI	GACNNNNNNGTC	1 cut(s)	1451
DseDI	GACNNNNNNGTC	1 cut(s)	1451
EaeI	YGGCCR	4 cut(s)	231, 713, 1015, 2155
EagI	CGGCCG	1 cut(s)	231
Eam1104I	CTCTTC	1 cut(s)	2189
EarI	CTCTTC	1 cut(s)	2189
EciI	GGCGGA	1 cut(s)	14
EclXI	CGGCCG	1 cut(s)	231
Eco130I	CCWWGG	4 cut(s)	716, 888, 1854, 2007
Eco32I	GATATC	2 cut(s)	500, 557
Eco47I	GGWCC	2 cut(s)	254, 1858
Eco52I	CGGCCG	1 cut(s)	231
Eco57I	CTGAAG	4 cut(s)	965, 1600, 1715, 2170
Eco81I	CCTNAGG	1 cut(s)	1986
Eco88I	CYCGRG	1 cut(s)	170
EcoRI	GAATTC	1 cut(s)	77
EcoRII	CCWGG	3 cut(s)	337, 493, 1570
EcoRV	GATATC	2 cut(s)	500, 557
EcoT14I	CCWWGG	4 cut(s)	716, 888, 1854, 2007
EcoT22I	ATGCAT	1 cut(s)	1248
ErhI	CCWWGG	4 cut(s)	716, 888, 1854, 2007
FalI	AAGNNNNNCTT	2 cut(s)	1131, 1163
FaqI	GGGAC	2 cut(s)	758, 2059
FauI	CCCGC	1 cut(s)	2116
FauNDI	CATATG	1 cut(s)	1595
FblI	GTMKAC	1 cut(s)	1455
FokI	GGATG	4 cut(s)	321, 978, 1844, 2054
FspBI	CTAG	6 cut(s)	1383, 1461, 1500, 1635, 2075, 2147
GlaI	GCGC	2 cut(s)	322, 2004
GsaI	CCCAGC	4 cut(s)	166, 830, 1870, 2016
GsuI	CTGGAG	2 cut(s)	1179, 1236
HaeII	RGCGCY	1 cut(s)	324
HaeIII	GGCC	8 cut(s)	86, 233, 242, 412, 715, 948, 1017, 2157
HapII	CCGG	5 cut(s)	28, 39, 58, 263, 416
HgaI	GACGC	3 cut(s)	209, 311, 778
HhaI	GCGC	2 cut(s)	323, 2005
Hin1I	GRCGYC	2 cut(s)	201, 303
Hin6I	GCGC	2 cut(s)	321, 2003
HinP1I	GCGC	2 cut(s)	321, 2003
HincII	GTYRAC	1 cut(s)	1456
HindII	GTYRAC	1 cut(s)	1456
HindIII	AAGCTT	3 cut(s)	636, 1268, 2027
HinfI	GANTC	8 cut(s)	112, 168, 175, 295, 616, 1143, 1161, 1620
HpaII	CCGG	5 cut(s)	28, 39, 58, 263, 416
HphI	GGTGA	4 cut(s)	452, 1185, 1318, 1418
Hpy166II	GTNNAC	6 cut(s)	193, 1349, 1456, 1480, 1849, 2134
Hpy188III	TCNNGA	9 cut(s)	58, 172, 299, 431, 515, 620, 793, 1925, 1949
Hpy8I	GTNNAC	6 cut(s)	193, 1349, 1456, 1480, 1849, 2134
Hpy99I	CGWCG	5 cut(s)	10, 188, 203, 239, 305
HpyAV	CCTTC	6 cut(s)	58, 1157, 1175, 1309, 1624, 2051
HpyCH4III	ACNGT	8 cut(s)	19, 70, 184, 919, 940, 1201, 1237, 1754
HpyCH4IV	ACGT	2 cut(s)	655, 1225
HpyF3I	CTNAG	7 cut(s)	272, 324, 943, 1002, 1266, 1840, 1986
HpySE526I	ACGT	2 cut(s)	655, 1225
Hsp92I	GRCGYC	2 cut(s)	201, 303
HspAI	GCGC	2 cut(s)	321, 2003
Kpn2I	TCCGGA	1 cut(s)	57
KspI	CCGCGG	1 cut(s)	98
Kzo9I	GATC	9 cut(s)	147, 511, 523, 735, 854, 1021, 1083, 1252, 1945
LmnI	GCTCC	5 cut(s)	647, 835, 1648, 1725, 2098
LweI	GCATC	2 cut(s)	428, 1000
MaeI	CTAG	6 cut(s)	1383, 1461, 1500, 1635, 2075, 2147
MaeII	ACGT	2 cut(s)	655, 1225
MaeIII	GTNAC	7 cut(s)	64, 506, 997, 1077, 1689, 1777, 1890
MalI	GATC	9 cut(s)	149, 513, 525, 737, 856, 1023, 1085, 1254, 1947
MboI	GATC	9 cut(s)	147, 511, 523, 735, 854, 1021, 1083, 1252, 1945
MflI	RGATCY	3 cut(s)	735, 1021, 1083
MhlI	GDGCHC	1 cut(s)	652
MlsI	TGGCCA	1 cut(s)	2157
MluNI	TGGCCA	1 cut(s)	2157
MlyI	GAGTC	4 cut(s)	162, 625, 1170, 1629
MmeI	TCCRAC	2 cut(s)	336, 675
Mox20I	TGGCCA	1 cut(s)	2157
Mph1103I	ATGCAT	1 cut(s)	1248
MroI	TCCGGA	1 cut(s)	57
MroXI	GAANNNNTTC	3 cut(s)	1062, 1142, 1566
MscI	TGGCCA	1 cut(s)	2157
MseI	TTAA	2 cut(s)	566, 1437
MslI	CAYNNNNRTG	5 cut(s)	585, 756, 771, 965, 1629
Msp20I	TGGCCA	1 cut(s)	2157
MspA1I	CMGCKG	4 cut(s)	97, 1523, 2123, 2153
MspI	CCGG	5 cut(s)	28, 39, 58, 263, 416
MspR9I	CCNGG	3 cut(s)	339, 495, 1572
MvaI	CCWGG	3 cut(s)	339, 495, 1572
MvnI	CGCG	2 cut(s)	97, 432
NcoI	CCATGG	1 cut(s)	2007
NdeI	CATATG	1 cut(s)	1595
NdeII	GATC	9 cut(s)	147, 511, 523, 735, 854, 1021, 1083, 1252, 1945
NheI	GCTAGC	1 cut(s)	1382
NlaIV	GGNNCC	2 cut(s)	483, 2115
NmeAIII	GCCGAG	1 cut(s)	62
NmuCI	GTSAC	3 cut(s)	997, 1077, 1890
NruI	TCGCGA	1 cut(s)	432
NsiI	ATGCAT	1 cut(s)	1248
NspI	RCATGY	2 cut(s)	1246, 2105
NspV	TTCGAA	1 cut(s)	1107
PaeI	GCATGC	1 cut(s)	1246
PcsI	WCGNNNNNNNCGW	1 cut(s)	306
PdmI	GAANNNNTTC	3 cut(s)	1062, 1142, 1566
PfeI	GAWTC	4 cut(s)	112, 175, 295, 1143
PflMI	CCANNNNNTGG	3 cut(s)	581, 1157, 1765
PleI	GAGTC	4 cut(s)	162, 624, 1169, 1628
PpsI	GAGTC	4 cut(s)	162, 624, 1169, 1628
PsiI	TTATAA	1 cut(s)	1713
Psp6I	CCWGG	3 cut(s)	337, 493, 1570
PspFI	CCCAGC	4 cut(s)	162, 826, 1866, 2012
PspGI	CCWGG	3 cut(s)	337, 493, 1570
PspN4I	GGNNCC	2 cut(s)	483, 2115
PspPI	GGNCC	3 cut(s)	254, 410, 1858
PstI	CTGCAG	2 cut(s)	146, 1300
PstNI	CAGNNNCTG	1 cut(s)	1157
PsuI	RGATCY	3 cut(s)	735, 1021, 1083
PvuII	CAGCTG	2 cut(s)	1523, 2153
RruI	TCGCGA	1 cut(s)	432
RsaI	GTAC	2 cut(s)	316, 1134
RsaNI	GTAC	2 cut(s)	315, 1133
RseI	CAYNNNNRTG	5 cut(s)	585, 756, 771, 965, 1629
SacII	CCGCGG	1 cut(s)	98
SalI	GTCGAC	1 cut(s)	1454
SaqAI	TTAA	2 cut(s)	566, 1437
Sau3AI	GATC	9 cut(s)	147, 511, 523, 735, 854, 1021, 1083, 1252, 1945
Sau96I	GGNCC	3 cut(s)	254, 410, 1858
SbfI	CCTGCAGG	1 cut(s)	146
SchI	GAGTC	4 cut(s)	162, 625, 1170, 1629
ScrFI	CCNGG	3 cut(s)	339, 495, 1572
SdaI	CCTGCAGG	1 cut(s)	146
SduI	GDGCHC	1 cut(s)	652
SfaNI	GCATC	2 cut(s)	428, 1000
SfcI	CTRYAG	2 cut(s)	142, 1296
SfiI	GGCCNNNNNGGCC	1 cut(s)	239
Sfr303I	CCGCGG	1 cut(s)	98
SfuI	TTCGAA	1 cut(s)	1107
SgrBI	CCGCGG	1 cut(s)	98
SinI	GGWCC	2 cut(s)	254, 1858
SmiMI	CAYNNNNRTG	5 cut(s)	585, 756, 771, 965, 1629
SmlI	CTYRAG	1 cut(s)	1652
SmoI	CTYRAG	1 cut(s)	1652
SpeI	ACTAGT	1 cut(s)	2074
SphI	GCATGC	1 cut(s)	1246
Sse8387I	CCTGCAGG	1 cut(s)	146
SsiI	CCGC	8 cut(s)	25, 95, 97, 154, 306, 473, 485, 2123
SspI	AATATT	1 cut(s)	690
SspMI	CTAG	6 cut(s)	1383, 1461, 1500, 1635, 2075, 2147
StyD4I	CCNGG	3 cut(s)	337, 493, 1570
StyI	CCWWGG	4 cut(s)	716, 888, 1854, 2007
TaaI	ACNGT	8 cut(s)	19, 70, 184, 919, 940, 1201, 1237, 1754
TaiI	ACGT	2 cut(s)	658, 1228
TaqI	TCGA	9 cut(s)	293, 300, 633, 853, 976, 1107, 1291, 1455, 1950
TauI	GCSGC	4 cut(s)	97, 308, 476, 487
TfiI	GAWTC	4 cut(s)	112, 175, 295, 1143
Tru1I	TTAA	2 cut(s)	566, 1437
Tru9I	TTAA	2 cut(s)	566, 1437
TscAI	CASTG	4 cut(s)	922, 1240, 1684, 1999
TseFI	GTSAC	3 cut(s)	997, 1077, 1890
Tsp45I	GTSAC	3 cut(s)	997, 1077, 1890
TspGWI	ACGGA	1 cut(s)	1068
TspRI	CASTG	4 cut(s)	922, 1240, 1684, 1999
Van91I	CCANNNNNTGG	3 cut(s)	581, 1157, 1765
VpaK11BI	GGWCC	2 cut(s)	254, 1858
XapI	RAATTY	5 cut(s)	77, 626, 1038, 1546, 1553
XceI	RCATGY	2 cut(s)	1246, 2105
XcmI	CCANNNNNNNNNTGG	2 cut(s)	433, 2014
XmiI	GTMKAC	1 cut(s)	1455
XmnI	GAANNNNTTC	3 cut(s)	1062, 1142, 1566
XspI	CTAG	6 cut(s)	1383, 1461, 1500, 1635, 2075, 2147
Zsp2I	ATGCAT	1 cut(s)	1248

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.