AT1G03250

R3H-associated N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013427
Physical Location & Seq
Reverse (-)
793315 .. 795403
2089 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G03250.3

Sequence Viewer

Length: 738 bp
ATGGCGGCTACCAATGTCATGCGGCGAGACGAGAGCCTTCTTATTGATCCCCAAAGAGGAGATACAAGTGTTTCTCGTGGCCTCTCGCTTGAGAAGAAAATTGAGGCCCTTGAGAGCCTGGCCGGACAAGTTAGTAACAGGCGATCTCGGAGATGGTTAAATGATCGTATTCTGATGGAACTTGTTCCTCGTTTGGATGCCCAAGAAATCAGAGGCTTGTTTGCTCCACCACCGTGGGGTGATGATGTCCCACCCTCAGCATTTTCTTTGACTAATGTTGGAGAATGGGACAAATTTAGAAACATAGACATGGACAAGGAGGCCAACATCATGGATTCCTTGAATAGGTCATCCGTAAGGCAGAAAGGTAGCGTGGACGGTGATAAAATCGCTGTCTTAAATGCTTGGAGAAGAATAGATTGTAGGACAAGAGATGCTCTTCGACGTAGCTTTTTACCAGAACTAATCGAGGGATATGAGAGCTGTATAAGTCACTTCATCGAGGAAGGTGGTGAAGGAGATGTTCTTGAGCTCAAGGTTCAAGACCCATTCCATAGATTGCTTTTACACGGTGTTTGTGAGTATCACAATTTGGTATCAACAACTGCAACAGAACAGATAGGAAGGATAGCAATGAAGACAACGAGCATCAAATGGAAGAAGAGTGGCGATTCAGGGGAGAAACCAAGCATCAGCCTAGCGCATTTCCTTAGGATGTCAAAGGAAGGAGCTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
R3H R3H-assoc

Protein Analysis

245

Amino Acids

27.72

Weight (kDa)

6.55

Isoelectric Point (pI)

55.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
R3H-assoc PF13902 1 - 91 6.5e-20 R3H-associated N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 22
AclWI GGATC 1 cut(s) 41
AcoI YGGCCR 1 cut(s) 120
AcsI RAATTY 1 cut(s) 293
AfiI CCNNNNNNNGG 3 cut(s) 56, 236, 345
AgsI TTSAA 2 cut(s) 343, 542
AjnI CCWGG 1 cut(s) 117
AleI CACNNNNGTG 1 cut(s) 232
AluBI AGCT 4 cut(s) 450, 483, 532, 731
AluI AGCT 4 cut(s) 450, 483, 532, 731
Alw21I GWGCWC 1 cut(s) 534
Alw26I GTCTC 1 cut(s) 21
AlwI GGATC 1 cut(s) 41
AoxI GGCC 4 cut(s) 79, 105, 120, 321
ApoI RAATTY 1 cut(s) 293
Asp700I GAANNNNTTC 1 cut(s) 183
AspLEI GCGC 1 cut(s) 703
AspS9I GGNCC 1 cut(s) 106
AsuHPI GGTGA 3 cut(s) 251, 392, 524
AxyI CCTNAGG 1 cut(s) 710
BaeI ACNNNNGTAYC 2 cut(s) 54, 87
BanII GRGCYC 1 cut(s) 534
BauI CACGAG 1 cut(s) 75
BbsI GAAGAC 1 cut(s) 644
Bbv12I GWGCWC 1 cut(s) 534
BbvCI CCTCAGC 1 cut(s) 256
BccI CCATC 2 cut(s) 147, 169
BciT130I CCWGG 1 cut(s) 119
BcoDI GTCTC 1 cut(s) 21
BfaI CTAG 1 cut(s) 698
BisI GCNGC 2 cut(s) 6, 23
BlsI GCNGC 2 cut(s) 7, 24
Bme1390I CCNGG 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 106
BmrFI CCNGG 1 cut(s) 119
BmsI GCATC 4 cut(s) 187, 424, 657, 699
BpiI GAAGAC 1 cut(s) 644
Bpu10I CCTNAGC 1 cut(s) 256
BpuEI CTTGAG 4 cut(s) 110, 131, 518, 548
BsaJI CCNNGG 1 cut(s) 233
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 236, 345
Bse21I CCTNAGG 1 cut(s) 710
Bse3DI GCAATG 1 cut(s) 639
BseBI CCWGG 1 cut(s) 119
BseDI CCNNGG 1 cut(s) 233
BseGI GGATG 3 cut(s) 202, 350, 720
BseLI CCNNNNNNNGG 3 cut(s) 56, 236, 345
BseMI GCAATG 1 cut(s) 639
BseMII CTCAG 1 cut(s) 270
BseRI GAGGAG 1 cut(s) 72
BshFI GGCC 4 cut(s) 81, 107, 122, 323
BsiHKAI GWGCWC 1 cut(s) 534
BsiSI CCGG 1 cut(s) 123
BslFI GGGAC 2 cut(s) 233, 302
BslI CCNNNNNNNGG 3 cut(s) 56, 236, 345
BsmAI GTCTC 1 cut(s) 21
BsmBI CGTCTC 1 cut(s) 21
BsmFI GGGAC 2 cut(s) 233, 302
BsnI GGCC 4 cut(s) 81, 107, 122, 323
Bsp1286I GDGCHC 1 cut(s) 534
Bsp143I GATC 3 cut(s) 46, 143, 163
BspACI CCGC 2 cut(s) 5, 22
BspANI GGCC 4 cut(s) 81, 107, 122, 323
BspCNI CTCAG 1 cut(s) 269
BspPI GGATC 1 cut(s) 41
BspQI GCTCTTC 1 cut(s) 444
BsrDI GCAATG 1 cut(s) 639
BssECI CCNNGG 1 cut(s) 233
BssMI GATC 3 cut(s) 46, 143, 163
BssSI CACGAG 1 cut(s) 75
Bst2BI CACGAG 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 119
Bst4CI ACNGT 3 cut(s) 234, 380, 572
Bst6I CTCTTC 2 cut(s) 444, 656
BstDEI CTNAG 2 cut(s) 256, 710
BstDSI CCRYGG 1 cut(s) 233
BstENI CCTNNNNNAGG 1 cut(s) 343
BstF5I GGATG 3 cut(s) 202, 350, 720
BstHHI GCGC 1 cut(s) 703
BstKTI GATC 3 cut(s) 49, 146, 166
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 3 cut(s) 46, 143, 163
BstNI CCWGG 1 cut(s) 119
BstSCI CCNGG 1 cut(s) 117
BstV2I GAAGAC 1 cut(s) 644
BstXI CCANNNNNNTGG 2 cut(s) 234, 331
Bsu36I CCTNAGG 1 cut(s) 710
BsuRI GGCC 4 cut(s) 81, 107, 122, 323
BtgI CCRYGG 1 cut(s) 233
BtsCI GGATG 3 cut(s) 202, 350, 720
CfoI GCGC 1 cut(s) 703
Cfr13I GGNCC 1 cut(s) 106
CviAII CATG 3 cut(s) 19, 310, 331
DdeI CTNAG 2 cut(s) 256, 710
DpnI GATC 3 cut(s) 48, 145, 165
DpnII GATC 3 cut(s) 46, 143, 163
EaeI YGGCCR 1 cut(s) 120
Eam1104I CTCTTC 2 cut(s) 444, 656
EarI CTCTTC 2 cut(s) 444, 656
Ecl136II GAGCTC 1 cut(s) 532
Eco24I GRGCYC 1 cut(s) 534
Eco53kI GAGCTC 1 cut(s) 532
Eco81I CCTNAGG 1 cut(s) 710
EcoICRI GAGCTC 1 cut(s) 532
EcoNI CCTNNNNNAGG 1 cut(s) 343
EcoO109I RGGNCCY 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 117
EcoT38I GRGCYC 1 cut(s) 534
Esp3I CGTCTC 1 cut(s) 21
FaeI CATG 3 cut(s) 22, 313, 334
FaiI YATR 7 cut(s) 20, 305, 311, 332, 477, 488, 555
FaqI GGGAC 2 cut(s) 233, 302
FatI CATG 3 cut(s) 18, 309, 330
Fnu4HI GCNGC 2 cut(s) 6, 23
FokI GGATG 3 cut(s) 209, 337, 727
FriOI GRGCYC 1 cut(s) 534
Fsp4HI GCNGC 2 cut(s) 6, 23
FspBI CTAG 1 cut(s) 698
GlaI GCGC 1 cut(s) 702
GluI GCNGC 2 cut(s) 6, 23
HaeIII GGCC 4 cut(s) 81, 107, 122, 323
HapII CCGG 1 cut(s) 123
HhaI GCGC 1 cut(s) 703
Hin1II CATG 3 cut(s) 22, 313, 334
Hin6I GCGC 1 cut(s) 701
HinP1I GCGC 1 cut(s) 701
HinfI GANTC 2 cut(s) 335, 671
HpaII CCGG 1 cut(s) 123
HphI GGTGA 3 cut(s) 251, 392, 524
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 3 cut(s) 150, 174, 212
Hpy188III TCNNGA 2 cut(s) 527, 542
Hpy8I GTNNAC 1 cut(s) 376
Hpy99I CGWCG 1 cut(s) 447
HpyAV CCTTC 5 cut(s) 47, 500, 509, 618, 719
HpyCH4III ACNGT 3 cut(s) 234, 380, 572
HpyCH4IV ACGT 1 cut(s) 445
HpyCH4V TGCA 1 cut(s) 608
HpyF3I CTNAG 2 cut(s) 256, 710
HpySE526I ACGT 1 cut(s) 445
Hsp92II CATG 3 cut(s) 22, 313, 334
HspAI GCGC 1 cut(s) 701
Kzo9I GATC 3 cut(s) 46, 143, 163
LguI GCTCTTC 1 cut(s) 444
LmnI GCTCC 2 cut(s) 229, 728
LpnPI CCDG 6 cut(s) 104, 124, 131, 136, 471, 660
LweI GCATC 4 cut(s) 187, 424, 657, 699
MaeI CTAG 1 cut(s) 698
MaeII ACGT 1 cut(s) 445
MaeIII GTNAC 2 cut(s) 134, 491
MalI GATC 3 cut(s) 48, 145, 165
MboI GATC 3 cut(s) 46, 143, 163
MboII GAAGA 6 cut(s) 106, 423, 431, 649, 670, 673
MhlI GDGCHC 1 cut(s) 534
MluCI AATT 3 cut(s) 99, 293, 589
MmeI TCCRAC 1 cut(s) 259
MnlI CCTC 9 cut(s) 50, 92, 97, 198, 206, 265, 313, 463, 496
MroXI GAANNNNTTC 1 cut(s) 183
MseI TTAA 2 cut(s) 158, 398
MslI CAYNNNNRTG 2 cut(s) 232, 308
MspI CCGG 1 cut(s) 123
MspR9I CCNGG 1 cut(s) 119
MvaI CCWGG 1 cut(s) 119
NdeII GATC 3 cut(s) 46, 143, 163
NlaIII CATG 3 cut(s) 22, 313, 334
NmuCI GTSAC 1 cut(s) 491
OliI CACNNNNGTG 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 444
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 2 cut(s) 335, 671
PkrI GCNGC 2 cut(s) 7, 24
Psp124BI GAGCTC 1 cut(s) 534
Psp6I CCWGG 1 cut(s) 117
PspGI CCWGG 1 cut(s) 117
PspPI GGNCC 1 cut(s) 106
RseI CAYNNNNRTG 2 cut(s) 232, 308
SacI GAGCTC 1 cut(s) 534
SapI GCTCTTC 1 cut(s) 444
SaqAI TTAA 2 cut(s) 158, 398
SatI GCNGC 2 cut(s) 6, 23
Sau3AI GATC 3 cut(s) 46, 143, 163
Sau96I GGNCC 1 cut(s) 106
ScrFI CCNGG 1 cut(s) 119
SduI GDGCHC 1 cut(s) 534
SetI ASST 9 cut(s) 350, 370, 448, 452, 485, 511, 534, 540, 733
SfaNI GCATC 4 cut(s) 187, 424, 657, 699
SmiMI CAYNNNNRTG 2 cut(s) 232, 308
SmlI CTYRAG 4 cut(s) 89, 110, 527, 533
SmoI CTYRAG 4 cut(s) 89, 110, 527, 533
Sse9I AATT 3 cut(s) 99, 293, 589
SsiI CCGC 2 cut(s) 5, 22
SspMI CTAG 1 cut(s) 698
SstI GAGCTC 1 cut(s) 534
StyD4I CCNGG 1 cut(s) 117
TaaI ACNGT 3 cut(s) 234, 380, 572
TaiI ACGT 1 cut(s) 448
TaqI TCGA 3 cut(s) 442, 468, 501
TasI AATT 3 cut(s) 99, 293, 589
TauI GCSGC 2 cut(s) 8, 25
TfiI GAWTC 2 cut(s) 335, 671
Tru1I TTAA 2 cut(s) 158, 398
Tru9I TTAA 2 cut(s) 158, 398
TseFI GTSAC 1 cut(s) 491
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 2 cut(s) 487, 650
TspGWI ACGGA 1 cut(s) 343
XagI CCTNNNNNAGG 1 cut(s) 343
XapI RAATTY 1 cut(s) 293
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.