MD11G1288600.v1.1

R3H-associated N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0013427
Physical Location & Seq
Reverse (-)
40823605 .. 40826658
3054 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1288600.v1.1.491

Sequence Viewer

Length: 1002 bp
ATGTGCCGTGTGGCATCTCCACAAAGTACAAGTATATTCTGGTGTGGGTTCTCTCCTTCCCACTCTTCCGTATTTGTTGCGTCCCTCTTATTTCTCAGCGCCTTTGAAAGGAACGTTTCGTCGCCCTTTATCTCTCTCATCTCTTTTCTCAAAATCTATCGCCGCAGAAAAAACCCAGAAGCCCAGAAGAAGAAGAAGAAGAATCAAAGCCGCAAGCTTTCAGATCAGATTTGCATCCAATCAATCATGGCGACTCCCGGCGTTCTCAACAGAGAAGACGGCTTTTTCCTCAACTCTCCAATCCTCGAACCTCTAAATGGAGAGAGTGTCAAATCCCGTGGAATGTCGATTGAGAAGAAGATTGAGCAGCTTGAGAGCATGGCTGGAACGGTTAGCAACCGACGGTCTCGAAGATGGGTAAATGATCGGATTTTGTTGGAACTTGTTCCTCGTCTAAATGCAGAAGAAATCAGAGGCTTGTTTGCTCCACCACCTTGGGGTGATGATGTACCGCTGTCAGCATTTTGCATGACTAATGTGCGAGAGTGGGATACATTTAGGAATATAGACATGGATAAGCAGGCTAATGTGATTGATTCCATGAAGGGCCCAAGTGCAAAGAAGAAGAGTCCTGTTGATGCTGATAAAACGGCCGTCTTGAATGCATGGCGTAGGGTTGATTGTCGAACAAGAGAGGCACTTCGCCGTGGCGTCTTTGCAGAACTTGTTGAGTTCTATGAGGAGCGTGTACGGGCTTTTGTTACGGAGACTGAAGATGGAGATGGAGATGTTCTCATCCTGCAGGTCCAAGATCCTCTCCGTCGATTGTTGCTCCATGGTGTTTGTGAGTTCTACAACTTGGTCTCGGTGACGGTTGCTGAATCGGAGAATGGGGAGTCAACTAAGATGACCAAGATAACGAAGAAAAAAGGGCGTCCGATCGAACTTCCAAAGATCACTCTGTCCCATTTCTTGAAGATGAACAAACAAGGAATGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
R3H R3H-assoc

Protein Analysis

334

Amino Acids

37.96

Weight (kDa)

9.54

Isoelectric Point (pI)

63.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
R3H-assoc PF13902 116 - 236 1.6e-32 R3H-associated N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 793
AciI CCGC 3 cut(s) 163, 211, 512
AclI AACGTT 1 cut(s) 114
AclWI GGATC 1 cut(s) 806
AcoI YGGCCR 1 cut(s) 651
AcuI CTGAAG 1 cut(s) 792
AcyI GRCGYC 2 cut(s) 711, 934
AfaI GTAC 3 cut(s) 28, 510, 750
AfiI CCNNNNNNNGG 3 cut(s) 108, 317, 497
AgsI TTSAA 3 cut(s) 107, 661, 976
AluBI AGCT 2 cut(s) 217, 370
AluI AGCT 2 cut(s) 217, 370
Alw26I GTCTC 3 cut(s) 411, 761, 868
AlwI GGATC 1 cut(s) 806
AoxI GGCC 2 cut(s) 607, 651
ApaI GGGCCC 1 cut(s) 611
ApeKI GCWGC 1 cut(s) 367
Asp700I GAANNNNTTC 1 cut(s) 444
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 3 cut(s) 607, 608, 805
AsuC2I CCSGG 1 cut(s) 258
AsuHPI GGTGA 2 cut(s) 512, 880
AvaII GGWCC 1 cut(s) 805
BaeGI GKGCMC 1 cut(s) 611
BanII GRGCYC 1 cut(s) 611
BbsI GAAGAC 1 cut(s) 282
BbvI GCAGC 1 cut(s) 379
BccI CCATC 3 cut(s) 408, 770, 776
BceAI ACGGC 4 cut(s) 295, 638, 666, 690
BciVI GTATCC 1 cut(s) 544
BcnI CCSGG 1 cut(s) 258
BcoDI GTCTC 3 cut(s) 411, 761, 868
BfmI CTRYAG 1 cut(s) 800
BfoI RGCGCY 1 cut(s) 102
BfuAI ACCTGC 1 cut(s) 793
BfuI GTATCC 1 cut(s) 544
BisI GCNGC 3 cut(s) 163, 211, 368
BlsI GCNGC 3 cut(s) 164, 212, 369
Bme1390I CCNGG 1 cut(s) 258
Bme18I GGWCC 1 cut(s) 805
BmgT120I GGNCC 3 cut(s) 607, 608, 805
BmiI GGNNCC 1 cut(s) 609
BmrFI CCNGG 1 cut(s) 258
BmsI GCATC 3 cut(s) 23, 243, 628
BpiI GAAGAC 1 cut(s) 282
BplI GAGNNNNNCTC 2 cut(s) 777, 809
BpuEI CTTGAG 1 cut(s) 392
BpuMI CCSGG 1 cut(s) 258
BsaBI GATNNNNATC 1 cut(s) 233
BsaHI GRCGYC 2 cut(s) 711, 934
BsaI GGTCTC 2 cut(s) 411, 868
BsaJI CCNNGG 4 cut(s) 337, 494, 706, 835
BsaXI ACNNNNNCTCC 2 cut(s) 312, 342
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 317, 497
Bse8I GATNNNNATC 1 cut(s) 233
BseDI CCNNGG 4 cut(s) 337, 494, 706, 835
BseGI GGATG 2 cut(s) 234, 795
BseJI GATNNNNATC 1 cut(s) 233
BseLI CCNNNNNNNGG 3 cut(s) 108, 317, 497
BseMII CTCAG 1 cut(s) 109
BseRI GAGGAG 1 cut(s) 755
BseSI GKGCMC 1 cut(s) 611
BseX3I CGGCCG 1 cut(s) 651
BseXI GCAGC 1 cut(s) 379
Bsh1285I CGRYCG 2 cut(s) 654, 942
BshFI GGCC 2 cut(s) 609, 653
BsiEI CGRYCG 2 cut(s) 654, 942
BsiSI CCGG 1 cut(s) 258
BslFI GGGAC 2 cut(s) 67, 949
BslI CCNNNNNNNGG 3 cut(s) 108, 317, 497
BsmAI GTCTC 3 cut(s) 411, 761, 868
BsmFI GGGAC 2 cut(s) 67, 949
BsmI GAATGC 1 cut(s) 667
BsnI GGCC 2 cut(s) 609, 653
Bso31I GGTCTC 2 cut(s) 411, 868
Bsp120I GGGCCC 1 cut(s) 607
Bsp1286I GDGCHC 1 cut(s) 611
Bsp143I GATC 5 cut(s) 223, 424, 811, 939, 954
Bsp19I CCATGG 1 cut(s) 835
BspACI CCGC 3 cut(s) 163, 211, 512
BspANI GGCC 2 cut(s) 609, 653
BspCNI CTCAG 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 609
BspMAI CTGCAG 1 cut(s) 804
BspMI ACCTGC 1 cut(s) 793
BspPI GGATC 1 cut(s) 806
BspTNI GGTCTC 2 cut(s) 411, 868
BssECI CCNNGG 4 cut(s) 337, 494, 706, 835
BssMI GATC 5 cut(s) 223, 424, 811, 939, 954
BssNI GRCGYC 2 cut(s) 711, 934
BssT1I CCWWGG 2 cut(s) 494, 835
Bst4CI ACNGT 3 cut(s) 391, 405, 874
Bst6I CTCTTC 2 cut(s) 70, 620
BstACI GRCGYC 2 cut(s) 711, 934
BstC8I GCNNGC 2 cut(s) 215, 582
BstDEI CTNAG 2 cut(s) 95, 903
BstDSI CCRYGG 3 cut(s) 337, 706, 835
BstENI CCTNNNNNAGG 1 cut(s) 106
BstF5I GGATG 2 cut(s) 234, 795
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 5 cut(s) 226, 427, 814, 942, 957
BstMAI GTCTC 3 cut(s) 411, 761, 868
BstMBI GATC 5 cut(s) 223, 424, 811, 939, 954
BstMCI CGRYCG 2 cut(s) 654, 942
BstSCI CCNGG 1 cut(s) 256
BstSFI CTRYAG 1 cut(s) 800
BstSLI GKGCMC 1 cut(s) 611
BstV1I GCAGC 1 cut(s) 379
BstV2I GAAGAC 1 cut(s) 282
BstX2I RGATCY 1 cut(s) 811
BstXI CCANNNNNNTGG 1 cut(s) 495
BstYI RGATCY 1 cut(s) 811
BstZI CGGCCG 1 cut(s) 651
BsuI GTATCC 1 cut(s) 544
BsuRI GGCC 2 cut(s) 609, 653
BtgI CCRYGG 3 cut(s) 337, 706, 835
BtsCI GGATG 2 cut(s) 234, 795
BveI ACCTGC 1 cut(s) 793
Cac8I GCNNGC 2 cut(s) 215, 582
CfoI GCGC 1 cut(s) 101
Cfr13I GGNCC 3 cut(s) 607, 608, 805
CseI GACGC 3 cut(s) 69, 700, 923
Csp6I GTAC 3 cut(s) 27, 509, 749
CspCI CAANNNNNGTGG 3 cut(s) 319, 354, 977
CviAII CATG 7 cut(s) 247, 379, 529, 571, 601, 666, 836
CviQI GTAC 3 cut(s) 27, 509, 749
DdeI CTNAG 2 cut(s) 95, 903
DpnI GATC 5 cut(s) 225, 426, 813, 941, 956
DpnII GATC 5 cut(s) 223, 424, 811, 939, 954
EaeI YGGCCR 1 cut(s) 651
EagI CGGCCG 1 cut(s) 651
Eam1104I CTCTTC 2 cut(s) 70, 620
EarI CTCTTC 2 cut(s) 70, 620
EclXI CGGCCG 1 cut(s) 651
Eco130I CCWWGG 2 cut(s) 494, 835
Eco24I GRGCYC 1 cut(s) 611
Eco31I GGTCTC 2 cut(s) 411, 868
Eco47I GGWCC 1 cut(s) 805
Eco52I CGGCCG 1 cut(s) 651
Eco57I CTGAAG 1 cut(s) 792
EcoNI CCTNNNNNAGG 1 cut(s) 106
EcoO109I RGGNCCY 1 cut(s) 607
EcoT14I CCWWGG 2 cut(s) 494, 835
EcoT22I ATGCAT 1 cut(s) 667
EcoT38I GRGCYC 1 cut(s) 611
ErhI CCWWGG 2 cut(s) 494, 835
FaeI CATG 7 cut(s) 250, 382, 532, 574, 604, 669, 839
FaqI GGGAC 2 cut(s) 67, 949
FatI CATG 7 cut(s) 246, 378, 528, 570, 600, 665, 835
Fnu4HI GCNGC 3 cut(s) 163, 211, 368
FokI GGATG 2 cut(s) 221, 782
FriOI GRGCYC 1 cut(s) 611
Fsp4HI GCNGC 3 cut(s) 163, 211, 368
GlaI GCGC 1 cut(s) 100
GluI GCNGC 3 cut(s) 163, 211, 368
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 2 cut(s) 609, 653
HapII CCGG 1 cut(s) 258
HgaI GACGC 3 cut(s) 69, 700, 923
HhaI GCGC 1 cut(s) 101
Hin1I GRCGYC 2 cut(s) 711, 934
Hin1II CATG 7 cut(s) 250, 382, 532, 574, 604, 669, 839
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HincII GTYRAC 1 cut(s) 900
HindII GTYRAC 1 cut(s) 900
HindIII AAGCTT 1 cut(s) 215
HinfI GANTC 6 cut(s) 202, 253, 596, 628, 881, 896
HpaII CCGG 1 cut(s) 258
HphI GGTGA 2 cut(s) 512, 880
Hpy166II GTNNAC 2 cut(s) 749, 900
Hpy188I TCNGA 6 cut(s) 223, 228, 429, 473, 886, 939
Hpy188III TCNNGA 3 cut(s) 408, 658, 973
Hpy8I GTNNAC 2 cut(s) 749, 900
Hpy99I CGWCG 3 cut(s) 124, 405, 825
HpyAV CCTTC 2 cut(s) 66, 598
HpyCH4III ACNGT 3 cut(s) 391, 405, 874
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 7 cut(s) 234, 461, 528, 617, 665, 719, 802
HpyF3I CTNAG 2 cut(s) 95, 903
HpySE526I ACGT 1 cut(s) 114
Hsp92I GRCGYC 2 cut(s) 711, 934
Hsp92II CATG 7 cut(s) 250, 382, 532, 574, 604, 669, 839
HspAI GCGC 1 cut(s) 99
Kzo9I GATC 5 cut(s) 223, 424, 811, 939, 954
LmnI GCTCC 3 cut(s) 490, 742, 837
LpnPI CCDG 9 cut(s) 25, 189, 197, 271, 369, 566, 645, 788, 812
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 3 cut(s) 23, 243, 628
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 2 cut(s) 760, 868
MalI GATC 5 cut(s) 225, 426, 813, 941, 956
MboI GATC 5 cut(s) 223, 424, 811, 939, 954
MflI RGATCY 1 cut(s) 811
MhlI GDGCHC 1 cut(s) 611
MlyI GAGTC 3 cut(s) 247, 637, 905
MmeI TCCRAC 1 cut(s) 417
MnlI CCTC 9 cut(s) 95, 299, 314, 321, 459, 467, 688, 733, 825
Mph1103I ATGCAT 1 cut(s) 667
MroXI GAANNNNTTC 1 cut(s) 444
MspA1I CMGCKG 1 cut(s) 514
MspI CCGG 1 cut(s) 258
MspR9I CCNGG 1 cut(s) 258
Mva1269I GAATGC 1 cut(s) 667
NciI CCSGG 1 cut(s) 258
NcoI CCATGG 1 cut(s) 835
NdeII GATC 5 cut(s) 223, 424, 811, 939, 954
NlaIII CATG 7 cut(s) 250, 382, 532, 574, 604, 669, 839
NlaIV GGNNCC 1 cut(s) 609
NmuCI GTSAC 1 cut(s) 868
NsiI ATGCAT 1 cut(s) 667
PctI GAATGC 1 cut(s) 667
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 3 cut(s) 202, 596, 881
PkrI GCNGC 3 cut(s) 164, 212, 369
Ple19I CGATCG 1 cut(s) 942
PleI GAGTC 3 cut(s) 247, 636, 904
PpsI GAGTC 3 cut(s) 247, 636, 904
Psp1406I AACGTT 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 609
PspOMI GGGCCC 1 cut(s) 607
PspPI GGNCC 3 cut(s) 607, 608, 805
PstI CTGCAG 1 cut(s) 804
PsuI RGATCY 1 cut(s) 811
PvuI CGATCG 1 cut(s) 942
RsaI GTAC 3 cut(s) 28, 510, 750
RsaNI GTAC 3 cut(s) 27, 509, 749
SatI GCNGC 3 cut(s) 163, 211, 368
Sau3AI GATC 5 cut(s) 223, 424, 811, 939, 954
Sau96I GGNCC 3 cut(s) 607, 608, 805
SbfI CCTGCAGG 1 cut(s) 804
SchI GAGTC 3 cut(s) 247, 637, 905
ScrFI CCNGG 1 cut(s) 258
SdaI CCTGCAGG 1 cut(s) 804
SduI GDGCHC 1 cut(s) 611
SetI ASST 6 cut(s) 117, 219, 313, 372, 496, 807
SfaNI GCATC 3 cut(s) 23, 243, 628
SfcI CTRYAG 1 cut(s) 800
SinI GGWCC 1 cut(s) 805
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse8387I CCTGCAGG 1 cut(s) 804
SsiI CCGC 3 cut(s) 163, 211, 512
StyD4I CCNGG 1 cut(s) 256
StyI CCWWGG 2 cut(s) 494, 835
TaaI ACNGT 3 cut(s) 391, 405, 874
TaiI ACGT 1 cut(s) 117
TaqI TCGA 6 cut(s) 306, 347, 409, 685, 823, 942
TatI WGTACW 1 cut(s) 26
TauI GCSGC 2 cut(s) 165, 213
TfiI GAWTC 3 cut(s) 202, 596, 881
TseFI GTSAC 1 cut(s) 868
TseI GCWGC 1 cut(s) 367
Tsp45I GTSAC 1 cut(s) 868
TspDTI ATGAA 2 cut(s) 617, 995
TspGWI ACGGA 3 cut(s) 58, 779, 809
VpaK11BI GGWCC 1 cut(s) 805
XagI CCTNNNNNAGG 1 cut(s) 106
XmnI GAANNNNTTC 1 cut(s) 444
Zsp2I ATGCAT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.