AT1G04640

Biotin/lipoate A/B protein ligase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011903
Physical Location & Seq
Forward (+)
1292023 .. 1293398
1376 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G04640.2

Sequence Viewer

Length: 708 bp
ATGAGATCCCCTAGAACCTTGGAGGTTTGGAAGCTAGGCACTGTCAACTATTTGAAATCCCTTAAACTGCAGGAGAAATTAGTTTCAGAGAGAAAAGCTCATCAGATTCCAGATACCCTCCTCTCGCTTCAGCATCCACCAACTTATACGCTCGGAAAGCGTAGAACCGATCACAATCTACTTATCCCTGAATCTGAACTTACAAAAATCGGAGCTGAACTTCATTATACTCAAAGAGGAGGAGACATCACCTTCCATGGCCCTCATCAAGCCATCTTATATCCCATCATTTCCTTACGCAGCATTGGTTTTGGTGCTAGGAACTACGTGGAGACATTGGAGCGGTCAATGATCGAGTTTGCTTCGATTTATGGCGTGAAAGCTCGAGCAGGAAACAAATGTGAGACTGGGGTTTGGGTTGGGGATAGGAAGATCGGTGCTATTGGGGTTAGGATATCTTCTGGAATCACTAGTCATGGTTTGGCCTTAAATATAGATCCTGATATGAAGTACTTTGAGCACATTGTGCCTTGTGGGATTGCTGATAAAGAAGTTACATCTTTGCGAAGAGAGACGGATACTCTGCTTCCTTCAGAAGAAGTGATTCATGAACAGTTGGTTTCTTGTTTAGCCAAAGCGTTTTCTTATGATGATGTTGTCTGGAAGGAAGATCCTTCACTCATTTTGGACACCCAAGATAAAGAATAA

Protein Analysis

235

Amino Acids

26.42

Weight (kDa)

6.51

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 16 - 203 1.2e-44 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011903)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04640 AT1G04640
fragaria_vesca FvH4_3g43830
malus_domestica MD11G1020800.v1.1
prunus_persica Prupe.6G017700_v2.0.a1
pyrus_communis pycom03g01630
rosa_chinensis RchiOBHm_Chr5g0079311
rosa_laevigata RLG00000036810
rosa_multiflora Rmu_sc0000607.1_g000005 Rmu_sc0002187.1_g000033
rosa_roxburghii Rroxscaffold_1G00003400 Rroxscaffold_1G00003620
rosa_rugosa Rorug05G0464300
rosa_samantha Rh5AG515200 Rh5BG540100 Rh5CG563100 Rh5DG550500
rosa_wichuraiana Rw0G005210 Rw5G048050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 343
AciI CCGC 1 cut(s) 343
AclWI GGATC 2 cut(s) 491, 665
AcuI CTGAAG 2 cut(s) 113, 576
AdeI CACNNNGTG 1 cut(s) 526
AfaI GTAC 1 cut(s) 512
AgsI TTSAA 1 cut(s) 55
AhlI ACTAGT 1 cut(s) 470
AluBI AGCT 4 cut(s) 34, 98, 215, 383
AluI AGCT 4 cut(s) 34, 98, 215, 383
Alw21I GWGCWC 1 cut(s) 522
Alw26I GTCTC 4 cut(s) 237, 326, 398, 566
AlwI GGATC 2 cut(s) 491, 665
Ama87I CYCGRG 1 cut(s) 384
AoxI GGCC 2 cut(s) 259, 483
ApeKI GCWGC 1 cut(s) 300
Asp700I GAANNNNTTC 1 cut(s) 603
AspS9I GGNCC 1 cut(s) 260
AsuHPI GGTGA 1 cut(s) 241
AvaI CYCGRG 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 522
BbvI GCAGC 1 cut(s) 312
BccI CCATC 2 cut(s) 281, 293
BciVI GTATCC 1 cut(s) 571
BcoDI GTCTC 4 cut(s) 237, 326, 398, 566
BcuI ACTAGT 1 cut(s) 470
BfaI CTAG 4 cut(s) 12, 35, 318, 471
BfmI CTRYAG 1 cut(s) 68
BfuI GTATCC 1 cut(s) 571
BisI GCNGC 1 cut(s) 301
BlsI GCNGC 1 cut(s) 302
BmcAI AGTACT 1 cut(s) 512
BmeT110I CYCGRG 1 cut(s) 384
BmgT120I GGNCC 1 cut(s) 260
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 1 cut(s) 142
BmuI ACTGGG 1 cut(s) 417
BplI GAGNNNNNCTC 2 cut(s) 82, 114
BsaAI YACGTR 1 cut(s) 328
BsaBI GATNNNNATC 1 cut(s) 174
BsaJI CCNNGG 2 cut(s) 18, 256
Bse1I ACTGG 1 cut(s) 412
Bse8I GATNNNNATC 1 cut(s) 174
BseDI CCNNGG 2 cut(s) 18, 256
BseGI GGATG 1 cut(s) 133
BseJI GATNNNNATC 1 cut(s) 174
BseNI ACTGG 1 cut(s) 412
BseRI GAGGAG 3 cut(s) 110, 252, 255
BseXI GCAGC 1 cut(s) 312
BshFI GGCC 2 cut(s) 261, 485
BsiHKAI GWGCWC 1 cut(s) 522
BsiHKCI CYCGRG 1 cut(s) 384
BsmAI GTCTC 4 cut(s) 237, 326, 398, 566
BsmBI CGTCTC 1 cut(s) 566
BsnI GGCC 2 cut(s) 261, 485
BsoBI CYCGRG 1 cut(s) 384
Bsp1286I GDGCHC 1 cut(s) 522
Bsp143I GATC 6 cut(s) 5, 169, 351, 432, 496, 670
Bsp19I CCATGG 1 cut(s) 256
BspACI CCGC 1 cut(s) 343
BspANI GGCC 2 cut(s) 261, 485
BspHI TCATGA 1 cut(s) 607
BspMAI CTGCAG 1 cut(s) 72
BspPI GGATC 2 cut(s) 491, 665
BsrBI CCGCTC 1 cut(s) 343
BsrI ACTGG 1 cut(s) 412
BssECI CCNNGG 2 cut(s) 18, 256
BssMI GATC 6 cut(s) 5, 169, 351, 432, 496, 670
BssT1I CCWWGG 2 cut(s) 18, 256
Bst4CI ACNGT 2 cut(s) 43, 615
Bst6I CTCTTC 1 cut(s) 562
BstAPI GCANNNNNTGC 1 cut(s) 526
BstBAI YACGTR 1 cut(s) 328
BstDSI CCRYGG 1 cut(s) 256
BstF5I GGATG 1 cut(s) 133
BstKTI GATC 6 cut(s) 8, 172, 354, 435, 499, 673
BstMAI GTCTC 4 cut(s) 237, 326, 398, 566
BstMBI GATC 6 cut(s) 5, 169, 351, 432, 496, 670
BstMWI GCNNNNNNNGC 2 cut(s) 157, 526
BstSFI CTRYAG 1 cut(s) 68
BstV1I GCAGC 1 cut(s) 312
BstX2I RGATCY 3 cut(s) 5, 496, 670
BstYI RGATCY 3 cut(s) 5, 496, 670
BsuI GTATCC 1 cut(s) 571
BsuRI GGCC 2 cut(s) 261, 485
BtgI CCRYGG 1 cut(s) 256
BtsCI GGATG 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 39
CciI TCATGA 1 cut(s) 607
Cfr13I GGNCC 1 cut(s) 260
Csp6I GTAC 1 cut(s) 511
CviAII CATG 3 cut(s) 257, 476, 608
CviJI RGCY 8 cut(s) 34, 98, 215, 261, 272, 383, 485, 632
CviKI_1 RGCY 8 cut(s) 34, 98, 215, 261, 272, 383, 485, 632
CviQI GTAC 1 cut(s) 511
DpnI GATC 6 cut(s) 7, 171, 353, 434, 498, 672
DpnII GATC 6 cut(s) 5, 169, 351, 432, 496, 670
DraIII CACNNNGTG 1 cut(s) 526
Eam1104I CTCTTC 1 cut(s) 562
EarI CTCTTC 1 cut(s) 562
Eco130I CCWWGG 2 cut(s) 18, 256
Eco32I GATATC 1 cut(s) 456
Eco57I CTGAAG 2 cut(s) 113, 576
Eco88I CYCGRG 1 cut(s) 384
EcoRV GATATC 1 cut(s) 456
EcoT14I CCWWGG 2 cut(s) 18, 256
ErhI CCWWGG 2 cut(s) 18, 256
Esp3I CGTCTC 1 cut(s) 566
FaeI CATG 3 cut(s) 260, 479, 611
FatI CATG 3 cut(s) 256, 475, 607
Fnu4HI GCNGC 1 cut(s) 301
FokI GGATG 1 cut(s) 120
Fsp4HI GCNGC 1 cut(s) 301
FspBI CTAG 4 cut(s) 12, 35, 318, 471
GluI GCNGC 1 cut(s) 301
HaeIII GGCC 2 cut(s) 261, 485
Hin1II CATG 3 cut(s) 260, 479, 611
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HinfI GANTC 4 cut(s) 106, 191, 465, 604
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 6 cut(s) 88, 105, 155, 196, 212, 595
Hpy188III TCNNGA 5 cut(s) 110, 462, 500, 608, 661
Hpy8I GTNNAC 1 cut(s) 46
HpyAV CCTTC 4 cut(s) 262, 600, 658, 684
HpyCH4III ACNGT 2 cut(s) 43, 615
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 1 cut(s) 70
HpyF10VI GCNNNNNNNGC 2 cut(s) 157, 526
HpySE526I ACGT 1 cut(s) 327
Hsp92II CATG 3 cut(s) 260, 479, 611
Kzo9I GATC 6 cut(s) 5, 169, 351, 432, 496, 670
LmnI GCTCC 2 cut(s) 212, 340
LpnPI CCDG 8 cut(s) 56, 123, 201, 375, 393, 447, 513, 646
Lsp1109I GCAGC 1 cut(s) 312
LweI GCATC 1 cut(s) 142
MaeI CTAG 4 cut(s) 12, 35, 318, 471
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 553
MalI GATC 6 cut(s) 7, 171, 353, 434, 498, 672
MbiI CCGCTC 1 cut(s) 343
MboI GATC 6 cut(s) 5, 169, 351, 432, 496, 670
MboII GAAGA 5 cut(s) 442, 450, 579, 608, 680
MflI RGATCY 3 cut(s) 5, 496, 670
MhlI GDGCHC 1 cut(s) 522
MluCI AATT 1 cut(s) 77
MnlI CCTC 6 cut(s) 16, 128, 131, 230, 233, 273
MroXI GAANNNNTTC 1 cut(s) 603
MseI TTAA 2 cut(s) 63, 488
MwoI GCNNNNNNNGC 2 cut(s) 157, 526
NcoI CCATGG 1 cut(s) 256
NdeII GATC 6 cut(s) 5, 169, 351, 432, 496, 670
NlaIII CATG 3 cut(s) 260, 479, 611
PaeR7I CTCGAG 1 cut(s) 384
PagI TCATGA 1 cut(s) 607
PdmI GAANNNNTTC 1 cut(s) 603
PfeI GAWTC 4 cut(s) 106, 191, 465, 604
PkrI GCNGC 1 cut(s) 302
Ppu21I YACGTR 1 cut(s) 328
PspPI GGNCC 1 cut(s) 260
PspXI VCTCGAGB 1 cut(s) 384
PstI CTGCAG 1 cut(s) 72
PsuI RGATCY 3 cut(s) 5, 496, 670
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
SaqAI TTAA 2 cut(s) 63, 488
SatI GCNGC 1 cut(s) 301
Sau3AI GATC 6 cut(s) 5, 169, 351, 432, 496, 670
Sau96I GGNCC 1 cut(s) 260
ScaI AGTACT 1 cut(s) 512
SduI GDGCHC 1 cut(s) 522
SetI ASST 8 cut(s) 20, 27, 36, 100, 217, 254, 330, 385
SfaNI GCATC 1 cut(s) 142
SfcI CTRYAG 1 cut(s) 68
Sfr274I CTCGAG 1 cut(s) 384
SlaI CTCGAG 1 cut(s) 384
SmlI CTYRAG 1 cut(s) 384
SmoI CTYRAG 1 cut(s) 384
SpeI ACTAGT 1 cut(s) 470
Sse9I AATT 1 cut(s) 77
SsiI CCGC 1 cut(s) 343
SspMI CTAG 4 cut(s) 12, 35, 318, 471
StyI CCWWGG 2 cut(s) 18, 256
TaaI ACNGT 2 cut(s) 43, 615
TaiI ACGT 1 cut(s) 330
TaqI TCGA 3 cut(s) 354, 365, 385
TasI AATT 1 cut(s) 77
TatI WGTACW 1 cut(s) 510
TfiI GAWTC 4 cut(s) 106, 191, 465, 604
Tru1I TTAA 2 cut(s) 63, 488
Tru9I TTAA 2 cut(s) 63, 488
TscAI CASTG 1 cut(s) 46
TseI GCWGC 1 cut(s) 300
TspDTI ATGAA 4 cut(s) 212, 521, 596, 624
TspGWI ACGGA 1 cut(s) 590
TspRI CASTG 1 cut(s) 46
XhoI CTCGAG 1 cut(s) 384
XmnI GAANNNNTTC 1 cut(s) 603
XspI CTAG 4 cut(s) 12, 35, 318, 471
ZrmI AGTACT 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.