Rh5DG550500

Biotin/lipoate A/B protein ligase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0011903
Physical Location & Seq
Reverse (-)
84675488 .. 84676966
1479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG550500.1

Sequence Viewer

Length: 711 bp
ATGAGAGTTCCGCGGACTCTTGAGGTCTGGAAACTGGGGACTATCAACTATCTGGAGGCACTTAAGCTGCAGGAGAAGCTGGTGTCCGATAGAAAGGTTCATAAGATTCCGGATACTCTCCTGGCCCTGCAGCATCCGCCGACTTATACCCTTGGCAAGCGGCGAACTGATCACAATCTGTTGATTCCTGAGTCTGATCTAAAAGGAATAGGAGCTGAACTGCACTATACGCAGAGAGGAGGAGACATTACATTTCATGGTCCGCGCCAGGCCATCTTGTATCCCATTGTTTCGCTTAGGGATATTGGGCTTGGGGCTCGGAGATATGTGGAGAAGCTTGAGTTGACTATGATTGAATTGGCGTCTCAGTATGGTGTGAAGGCTTGTCCGGGGCAAGCAGGAGAGACTGGGGTGTGGGTTGGAGAGAGAAAGATCGGTGCAATTGGCGTGCGCATTTCTTATGGAGTGACGTCTCATGGGTTGGCATTCAACATTGATCCGGATTTGAACTATTTCAAGCATATTGTGCCGTGCGGTATTGCTGATAAAGAAGTTACGTCTCTGAGAAAGGAGACAGACTCGGCGCTTCCTACAGAAGAAGTAGTTCAGGACCAGTTGATTTCTTGCTTTGCAAGATTGTTTGGTTATGGTAATGTTGTGTGGAAGGATTCTGCTTCTTCATTATTGTCAGACAGTGGGGAAATCAAATGA

Protein Analysis

236

Amino Acids

26.18

Weight (kDa)

7.74

Isoelectric Point (pI)

31.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 16 - 203 4.6e-44 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011903)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04640 AT1G04640
fragaria_vesca FvH4_3g43830
malus_domestica MD11G1020800.v1.1
prunus_persica Prupe.6G017700_v2.0.a1
pyrus_communis pycom03g01630
rosa_chinensis RchiOBHm_Chr5g0079311
rosa_laevigata RLG00000036810
rosa_multiflora Rmu_sc0000607.1_g000005 Rmu_sc0002187.1_g000033
rosa_roxburghii Rroxscaffold_1G00003400 Rroxscaffold_1G00003620
rosa_rugosa Rorug05G0464300
rosa_samantha Rh5AG515200 Rh5BG540100 Rh5CG563100 Rh5DG550500
rosa_wichuraiana Rw0G005210 Rw5G048050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 473
Acc16I TGCGCA 1 cut(s) 452
AccII CGCG 2 cut(s) 13, 265
AccIII TCCGGA 2 cut(s) 109, 499
AciI CCGC 6 cut(s) 11, 13, 137, 160, 263, 534
AclWI GGATC 1 cut(s) 491
AcyI GRCGYC 2 cut(s) 362, 470
AflII CTTAAG 1 cut(s) 62
AgsI TTSAA 4 cut(s) 356, 490, 508, 517
AjnI CCWGG 2 cut(s) 120, 267
AluBI AGCT 4 cut(s) 67, 79, 215, 337
AluI AGCT 4 cut(s) 67, 79, 215, 337
Alw26I GTCTC 6 cut(s) 237, 369, 398, 477, 564, 566
AlwI GGATC 1 cut(s) 491
Aor13HI TCCGGA 2 cut(s) 109, 499
AoxI GGCC 2 cut(s) 123, 270
ApeKI GCWGC 2 cut(s) 67, 130
Asp700I GAANNNNTTC 2 cut(s) 512, 603
AspLEI GCGC 3 cut(s) 267, 453, 586
AspS9I GGNCC 3 cut(s) 124, 260, 610
AsuC2I CCSGG 1 cut(s) 390
AvaII GGWCC 2 cut(s) 260, 610
BanII GRGCYC 1 cut(s) 319
BbvI GCAGC 2 cut(s) 54, 142
BccI CCATC 1 cut(s) 281
BceAI ACGGC 1 cut(s) 514
BciT130I CCWGG 2 cut(s) 122, 269
BciVI GTATCC 2 cut(s) 106, 291
BclI TGATCA 1 cut(s) 169
BcnI CCSGG 1 cut(s) 390
BcoDI GTCTC 6 cut(s) 237, 369, 398, 477, 564, 566
BfmI CTRYAG 3 cut(s) 68, 128, 591
BfoI RGCGCY 1 cut(s) 587
BfrI CTTAAG 1 cut(s) 62
BfuI GTATCC 2 cut(s) 106, 291
BisI GCNGC 3 cut(s) 68, 131, 161
BlsI GCNGC 3 cut(s) 69, 132, 162
Bme1390I CCNGG 3 cut(s) 122, 269, 390
Bme18I GGWCC 2 cut(s) 260, 610
BmgT120I GGNCC 3 cut(s) 124, 260, 610
BmrFI CCNGG 3 cut(s) 122, 269, 390
BmrI ACTGGG 2 cut(s) 44, 417
BmsI GCATC 1 cut(s) 142
BmuI ACTGGG 2 cut(s) 44, 417
BplI GAGNNNNNCTC 2 cut(s) 563, 595
BpmI CTGGAG 1 cut(s) 74
Bpu10I CCTNAGC 1 cut(s) 296
BpuEI CTTGAG 2 cut(s) 41, 359
BpuMI CCSGG 1 cut(s) 390
BsaBI GATNNNNATC 1 cut(s) 174
BsaHI GRCGYC 2 cut(s) 362, 470
BsaJI CCNNGG 3 cut(s) 11, 151, 389
BsaWI WCCGGW 2 cut(s) 109, 499
Bse1I ACTGG 3 cut(s) 39, 412, 613
Bse8I GATNNNNATC 1 cut(s) 174
BseAI TCCGGA 2 cut(s) 109, 499
BseBI CCWGG 2 cut(s) 122, 269
BseDI CCNNGG 3 cut(s) 11, 151, 389
BseGI GGATG 1 cut(s) 133
BseJI GATNNNNATC 1 cut(s) 174
BseMII CTCAG 3 cut(s) 180, 380, 554
BseNI ACTGG 3 cut(s) 39, 412, 613
BseRI GAGGAG 2 cut(s) 252, 255
BseXI GCAGC 2 cut(s) 54, 142
BsgI GTGCAG 1 cut(s) 206
Bsh1236I CGCG 2 cut(s) 13, 265
BshFI GGCC 2 cut(s) 125, 272
BsiSI CCGG 3 cut(s) 110, 389, 500
BslFI GGGAC 1 cut(s) 52
BsmAI GTCTC 6 cut(s) 237, 369, 398, 477, 564, 566
BsmBI CGTCTC 3 cut(s) 369, 477, 564
BsmFI GGGAC 1 cut(s) 52
BsmI GAATGC 1 cut(s) 485
BsnI GGCC 2 cut(s) 125, 272
Bsp1286I GDGCHC 1 cut(s) 319
Bsp13I TCCGGA 2 cut(s) 109, 499
Bsp143I GATC 4 cut(s) 169, 196, 432, 496
BspACI CCGC 6 cut(s) 11, 13, 137, 160, 263, 534
BspANI GGCC 2 cut(s) 125, 272
BspCNI CTCAG 3 cut(s) 181, 379, 555
BspEI TCCGGA 2 cut(s) 109, 499
BspFNI CGCG 2 cut(s) 13, 265
BspMAI CTGCAG 2 cut(s) 72, 132
BspPI GGATC 1 cut(s) 491
BspTI CTTAAG 1 cut(s) 62
BsrI ACTGG 3 cut(s) 39, 412, 613
BssECI CCNNGG 3 cut(s) 11, 151, 389
BssMI GATC 4 cut(s) 169, 196, 432, 496
BssNI GRCGYC 2 cut(s) 362, 470
BssT1I CCWWGG 1 cut(s) 151
Bst2UI CCWGG 2 cut(s) 122, 269
Bst4CI ACNGT 1 cut(s) 695
BstACI GRCGYC 2 cut(s) 362, 470
BstAFI CTTAAG 1 cut(s) 62
BstAPI GCANNNNNTGC 1 cut(s) 526
BstC8I GCNNGC 3 cut(s) 158, 396, 449
BstDEI CTNAG 4 cut(s) 189, 296, 366, 563
BstDSI CCRYGG 1 cut(s) 11
BstF5I GGATG 1 cut(s) 133
BstFNI CGCG 2 cut(s) 13, 265
BstH2I RGCGCY 1 cut(s) 587
BstHHI GCGC 3 cut(s) 267, 453, 586
BstKTI GATC 4 cut(s) 172, 199, 435, 499
BstMAI GTCTC 6 cut(s) 237, 369, 398, 477, 564, 566
BstMBI GATC 4 cut(s) 169, 196, 432, 496
BstMWI GCNNNNNNNGC 4 cut(s) 76, 136, 229, 526
BstNI CCWGG 2 cut(s) 122, 269
BstSCI CCNGG 3 cut(s) 120, 267, 388
BstSFI CTRYAG 3 cut(s) 68, 128, 591
BstUI CGCG 2 cut(s) 13, 265
BstV1I GCAGC 2 cut(s) 54, 142
BsuI GTATCC 2 cut(s) 106, 291
BsuRI GGCC 2 cut(s) 125, 272
BtgI CCRYGG 1 cut(s) 11
BtsCI GGATG 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 700
Cac8I GCNNGC 3 cut(s) 158, 396, 449
CfoI GCGC 3 cut(s) 267, 453, 586
Cfr13I GGNCC 3 cut(s) 124, 260, 610
Cfr42I CCGCGG 1 cut(s) 14
CseI GACGC 1 cut(s) 351
CviAII CATG 2 cut(s) 257, 476
CviJI RGCY 9 cut(s) 67, 79, 125, 215, 272, 310, 317, 337, 383
CviKI_1 RGCY 9 cut(s) 67, 79, 125, 215, 272, 310, 317, 337, 383
DdeI CTNAG 4 cut(s) 189, 296, 366, 563
DpnI GATC 4 cut(s) 171, 198, 434, 498
DpnII GATC 4 cut(s) 169, 196, 432, 496
EciI GGCGGA 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 151
Eco24I GRGCYC 1 cut(s) 319
Eco47I GGWCC 2 cut(s) 260, 610
EcoRII CCWGG 2 cut(s) 120, 267
EcoT14I CCWWGG 1 cut(s) 151
EcoT38I GRGCYC 1 cut(s) 319
ErhI CCWWGG 1 cut(s) 151
Esp3I CGTCTC 3 cut(s) 369, 477, 564
FaeI CATG 2 cut(s) 260, 479
FaqI GGGAC 1 cut(s) 52
FatI CATG 2 cut(s) 256, 475
FbaI TGATCA 1 cut(s) 169
Fnu4HI GCNGC 3 cut(s) 68, 131, 161
FokI GGATG 1 cut(s) 120
FriOI GRGCYC 1 cut(s) 319
Fsp4HI GCNGC 3 cut(s) 68, 131, 161
FspAI RTGCGCAY 1 cut(s) 452
FspI TGCGCA 1 cut(s) 452
GlaI GCGC 3 cut(s) 266, 452, 585
GluI GCNGC 3 cut(s) 68, 131, 161
GsuI CTGGAG 1 cut(s) 74
HaeII RGCGCY 1 cut(s) 587
HaeIII GGCC 2 cut(s) 125, 272
HapII CCGG 3 cut(s) 110, 389, 500
HgaI GACGC 1 cut(s) 351
HhaI GCGC 3 cut(s) 267, 453, 586
Hin1I GRCGYC 2 cut(s) 362, 470
Hin1II CATG 2 cut(s) 260, 479
Hin6I GCGC 3 cut(s) 265, 451, 584
HinP1I GCGC 3 cut(s) 265, 451, 584
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 6 cut(s) 16, 106, 184, 191, 578, 668
HpaII CCGG 3 cut(s) 110, 389, 500
Hpy166II GTNNAC 1 cut(s) 345
Hpy188I TCNGA 5 cut(s) 88, 196, 321, 564, 691
Hpy188III TCNNGA 7 cut(s) 20, 28, 53, 110, 188, 500, 608
Hpy8I GTNNAC 1 cut(s) 345
HpyAV CCTTC 2 cut(s) 373, 658
HpyCH4III ACNGT 1 cut(s) 695
HpyCH4IV ACGT 2 cut(s) 470, 557
HpyCH4V TGCA 5 cut(s) 70, 130, 223, 440, 632
HpyF10VI GCNNNNNNNGC 4 cut(s) 76, 136, 229, 526
HpyF3I CTNAG 4 cut(s) 189, 296, 366, 563
HpySE526I ACGT 2 cut(s) 470, 557
Hsp92I GRCGYC 2 cut(s) 362, 470
Hsp92II CATG 2 cut(s) 260, 479
HspAI GCGC 3 cut(s) 265, 451, 584
Kpn2I TCCGGA 2 cut(s) 109, 499
Ksp22I TGATCA 1 cut(s) 169
KspI CCGCGG 1 cut(s) 14
Kzo9I GATC 4 cut(s) 169, 196, 432, 496
LmnI GCTCC 1 cut(s) 212
Lsp1109I GCAGC 2 cut(s) 54, 142
LweI GCATC 1 cut(s) 142
MaeII ACGT 2 cut(s) 470, 557
MaeIII GTNAC 2 cut(s) 466, 553
MalI GATC 4 cut(s) 171, 198, 434, 498
MboI GATC 4 cut(s) 169, 196, 432, 496
MboII GAAGA 2 cut(s) 608, 669
MfeI CAATTG 1 cut(s) 441
MhlI GDGCHC 1 cut(s) 319
MluCI AATT 2 cut(s) 356, 441
MlyI GAGTC 3 cut(s) 10, 200, 572
MmeI TCCRAC 1 cut(s) 400
MnlI CCTC 4 cut(s) 16, 49, 230, 233
MroI TCCGGA 2 cut(s) 109, 499
MroXI GAANNNNTTC 2 cut(s) 512, 603
MseI TTAA 1 cut(s) 63
MspA1I CMGCKG 1 cut(s) 13
MspCI CTTAAG 1 cut(s) 62
MspI CCGG 3 cut(s) 110, 389, 500
MspR9I CCNGG 3 cut(s) 122, 269, 390
MunI CAATTG 1 cut(s) 441
Mva1269I GAATGC 1 cut(s) 485
MvaI CCWGG 2 cut(s) 122, 269
MvnI CGCG 2 cut(s) 13, 265
MwoI GCNNNNNNNGC 4 cut(s) 76, 136, 229, 526
NciI CCSGG 1 cut(s) 390
NdeII GATC 4 cut(s) 169, 196, 432, 496
NlaIII CATG 2 cut(s) 260, 479
NmeAIII GCCGAG 1 cut(s) 560
NmuCI GTSAC 1 cut(s) 466
NsbI TGCGCA 1 cut(s) 452
PctI GAATGC 1 cut(s) 485
PdmI GAANNNNTTC 2 cut(s) 512, 603
PfeI GAWTC 3 cut(s) 106, 184, 668
PkrI GCNGC 3 cut(s) 69, 132, 162
PleI GAGTC 3 cut(s) 10, 199, 572
PpsI GAGTC 3 cut(s) 10, 199, 572
Psp6I CCWGG 2 cut(s) 120, 267
PspGI CCWGG 2 cut(s) 120, 267
PspPI GGNCC 3 cut(s) 124, 260, 610
PstI CTGCAG 2 cut(s) 72, 132
SacII CCGCGG 1 cut(s) 14
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 3 cut(s) 68, 131, 161
Sau3AI GATC 4 cut(s) 169, 196, 432, 496
Sau96I GGNCC 3 cut(s) 124, 260, 610
SchI GAGTC 3 cut(s) 10, 200, 572
ScrFI CCNGG 3 cut(s) 122, 269, 390
SduI GDGCHC 1 cut(s) 319
SetI ASST 8 cut(s) 27, 69, 81, 99, 217, 339, 473, 560
SfaNI GCATC 1 cut(s) 142
SfcI CTRYAG 3 cut(s) 68, 128, 591
Sfr303I CCGCGG 1 cut(s) 14
SgrBI CCGCGG 1 cut(s) 14
SinI GGWCC 2 cut(s) 260, 610
SmlI CTYRAG 3 cut(s) 20, 62, 338
SmoI CTYRAG 3 cut(s) 20, 62, 338
Sse9I AATT 2 cut(s) 356, 441
SsiI CCGC 6 cut(s) 11, 13, 137, 160, 263, 534
StyD4I CCNGG 3 cut(s) 120, 267, 388
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 1 cut(s) 695
TaiI ACGT 2 cut(s) 473, 560
TasI AATT 2 cut(s) 356, 441
TauI GCSGC 1 cut(s) 163
TfiI GAWTC 3 cut(s) 106, 184, 668
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TscAI CASTG 1 cut(s) 700
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 2 cut(s) 67, 130
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 3 cut(s) 89, 245, 669
TspRI CASTG 1 cut(s) 700
Vha464I CTTAAG 1 cut(s) 62
VpaK11BI GGWCC 2 cut(s) 260, 610
XmnI GAANNNNTTC 2 cut(s) 512, 603
ZraI GACGTC 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.