pycom03g01630

Biotin/lipoate A/B protein ligase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0011903
Physical Location & Seq
Forward (+)
1212656 .. 1214711
2056 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g01630.3

Sequence Viewer

Length: 798 bp
ATGATTGCGGAAAGTGCTGTCACAGCCAGCAAAGTTTGGGTGGCTCGGGGACTTCTCAATCTCTCTGCAGTGGCTGGTCCAGCTTTCGGAATGAGAGTTCCACGAAATCTTGAGGTCTTGAGAATGGGCACCATGAGCTATCAGGAAGCACTTAAGCTGCAGGAAAAACTGGTTGCTGAAAGAAAAATTCATAAGATTCCAGATACTCTCGTGTCCCTGCAACATCCACCTACATACACCCTTGGCAAACGAAGAACTGATCACAATCTGTTGATTCCTGAGACTGAACTCAAAAGAATAGGAGCTCAACTGCACTACACACAAAGAGGAGGAGACATTACATATCACGGTCCACATCAAGCCATTTTATATCCCATTATTGCTCTTCGGGATATGGGGCTTGGTGCTCGTAAATATGTGGAGAAACTTGAGTCGACTATGATTGACTTGGCATCTCTGTATGGCGTAAAAGCTTGTCCTGGAAATAAATGTGAAACGGGGGTTTGGGTTGGAGATAGAAAAATCGGAGCAATTGGTGTACGGATTTCATATGGAATCACCTCTCATGGGTTGGCATTCAACATTGATCCAGATTTGAACTATTTCAAGCATATTGTGCCTTGTGGCATTGCAAATAAAGACGTTACATCATTGAGAAGGGAGACGGACTTAGCGCTTCCTACCGAAGAAGTAGTTCAGGACCAGTTGGTTTCTTGTTTTGCAAGACAATTTGGTTATAATGATCTTGTATGGAAGGATGCTGCTTCAATATTGTCAGAGAGCGGGGAAACTGAATGA

Protein Analysis

266

Amino Acids

29.31

Weight (kDa)

8.27

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011903)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04640 AT1G04640
fragaria_vesca FvH4_3g43830
malus_domestica MD11G1020800.v1.1
prunus_persica Prupe.6G017700_v2.0.a1
pyrus_communis pycom03g01630
rosa_chinensis RchiOBHm_Chr5g0079311
rosa_laevigata RLG00000036810
rosa_multiflora Rmu_sc0000607.1_g000005 Rmu_sc0002187.1_g000033
rosa_roxburghii Rroxscaffold_1G00003400 Rroxscaffold_1G00003620
rosa_rugosa Rorug05G0464300
rosa_samantha Rh5AG515200 Rh5BG540100 Rh5CG563100 Rh5DG550500
rosa_wichuraiana Rw0G005210 Rw5G048050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 738
AccB1I GGYRCC 1 cut(s) 128
AccBSI CCGCTC 1 cut(s) 783
AccI GTMKAC 1 cut(s) 434
AciI CCGC 2 cut(s) 8, 783
AclWI GGATC 1 cut(s) 581
AcsI RAATTY 1 cut(s) 186
AfaI GTAC 1 cut(s) 540
AfeI AGCGCT 1 cut(s) 675
AfiI CCNNNNNNNGG 2 cut(s) 86, 567
AflII CTTAAG 1 cut(s) 152
AgsI TTSAA 4 cut(s) 580, 598, 607, 768
AjnI CCWGG 1 cut(s) 478
AloI GAACNNNNNNTCC 2 cut(s) 81, 113
AluBI AGCT 5 cut(s) 83, 138, 157, 305, 473
AluI AGCT 5 cut(s) 83, 138, 157, 305, 473
Alw21I GWGCWC 2 cut(s) 307, 409
Alw26I GTCTC 3 cut(s) 275, 327, 656
AlwI GGATC 1 cut(s) 581
AlwNI CAGNNNCTG 1 cut(s) 74
Ama87I CYCGRG 1 cut(s) 45
Aor51HI AGCGCT 1 cut(s) 675
ApeKI GCWGC 2 cut(s) 157, 761
ApoI RAATTY 1 cut(s) 186
Asp700I GAANNNNTTC 2 cut(s) 602, 693
AspLEI GCGC 1 cut(s) 676
AspS9I GGNCC 3 cut(s) 77, 350, 700
AsuHPI GGTGA 1 cut(s) 550
AvaI CYCGRG 1 cut(s) 45
AvaII GGWCC 3 cut(s) 77, 350, 700
BaeGI GKGCMC 1 cut(s) 131
BaeI ACNNNNGTAYC 2 cut(s) 195, 228
BanI GGYRCC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 307
BauI CACGAG 1 cut(s) 209
Bbv12I GWGCWC 2 cut(s) 307, 409
BbvI GCAGC 2 cut(s) 144, 748
BciT130I CCWGG 1 cut(s) 480
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 3 cut(s) 275, 327, 656
BfmI CTRYAG 2 cut(s) 66, 158
BfoI RGCGCY 1 cut(s) 677
BfrI CTTAAG 1 cut(s) 152
BisI GCNGC 2 cut(s) 158, 762
BlsI GCNGC 2 cut(s) 159, 763
Bme1390I CCNGG 1 cut(s) 480
Bme18I GGWCC 3 cut(s) 77, 350, 700
BmeT110I CYCGRG 1 cut(s) 45
BmgT120I GGNCC 3 cut(s) 77, 350, 700
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 1 cut(s) 480
BmsI GCATC 2 cut(s) 461, 748
BpuEI CTTGAG 3 cut(s) 131, 139, 449
BsaBI GATNNNNATC 1 cut(s) 264
BsaJI CCNNGG 1 cut(s) 241
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 567
Bse1I ACTGG 2 cut(s) 174, 703
Bse3DI GCAATG 1 cut(s) 627
Bse8I GATNNNNATC 1 cut(s) 264
BseBI CCWGG 1 cut(s) 480
BseDI CCNNGG 1 cut(s) 241
BseGI GGATG 2 cut(s) 223, 763
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 2 cut(s) 86, 567
BseMI GCAATG 1 cut(s) 627
BseMII CTCAG 1 cut(s) 270
BseNI ACTGG 2 cut(s) 174, 703
BseRI GAGGAG 2 cut(s) 342, 345
BseSI GKGCMC 1 cut(s) 131
BseXI GCAGC 2 cut(s) 144, 748
BsgI GTGCAG 1 cut(s) 296
BshNI GGYRCC 1 cut(s) 128
BsiHKAI GWGCWC 2 cut(s) 307, 409
BsiHKCI CYCGRG 1 cut(s) 45
BslFI GGGAC 2 cut(s) 63, 199
BslI CCNNNNNNNGG 2 cut(s) 86, 567
BsmAI GTCTC 3 cut(s) 275, 327, 656
BsmBI CGTCTC 1 cut(s) 656
BsmFI GGGAC 2 cut(s) 63, 199
BsmI GAATGC 1 cut(s) 575
BsoBI CYCGRG 1 cut(s) 45
Bsp1286I GDGCHC 3 cut(s) 131, 307, 409
Bsp143I GATC 3 cut(s) 259, 586, 742
BspACI CCGC 2 cut(s) 8, 783
BspCNI CTCAG 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 130
BspMAI CTGCAG 2 cut(s) 70, 162
BspPI GGATC 1 cut(s) 581
BspQI GCTCTTC 1 cut(s) 390
BspT107I GGYRCC 1 cut(s) 128
BspTI CTTAAG 1 cut(s) 152
BsrBI CCGCTC 1 cut(s) 783
BsrDI GCAATG 1 cut(s) 627
BsrI ACTGG 2 cut(s) 174, 703
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 3 cut(s) 259, 586, 742
BssSI CACGAG 1 cut(s) 209
BssT1I CCWWGG 1 cut(s) 241
Bst2BI CACGAG 1 cut(s) 209
Bst2UI CCWGG 1 cut(s) 480
Bst4CI ACNGT 1 cut(s) 350
Bst6I CTCTTC 1 cut(s) 390
BstAFI CTTAAG 1 cut(s) 152
BstAPI GCANNNNNTGC 1 cut(s) 616
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 2 cut(s) 279, 670
BstF5I GGATG 2 cut(s) 223, 763
BstH2I RGCGCY 1 cut(s) 677
BstHHI GCGC 1 cut(s) 676
BstKTI GATC 3 cut(s) 262, 589, 745
BstMAI GTCTC 3 cut(s) 275, 327, 656
BstMBI GATC 3 cut(s) 259, 586, 742
BstMWI GCNNNNNNNGC 5 cut(s) 14, 23, 80, 135, 616
BstNI CCWGG 1 cut(s) 480
BstSCI CCNGG 1 cut(s) 478
BstSFI CTRYAG 2 cut(s) 66, 158
BstSLI GKGCMC 1 cut(s) 131
BstV1I GCAGC 2 cut(s) 144, 748
BtsCI GGATG 2 cut(s) 223, 763
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 28
CaiI CAGNNNCTG 1 cut(s) 74
CfoI GCGC 1 cut(s) 676
Cfr13I GGNCC 3 cut(s) 77, 350, 700
Csp6I GTAC 1 cut(s) 539
CviAII CATG 2 cut(s) 133, 566
CviQI GTAC 1 cut(s) 539
DdeI CTNAG 2 cut(s) 279, 670
DpnI GATC 3 cut(s) 261, 588, 744
DpnII GATC 3 cut(s) 259, 586, 742
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
Ecl136II GAGCTC 1 cut(s) 305
Eco130I CCWWGG 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 307
Eco47I GGWCC 3 cut(s) 77, 350, 700
Eco47III AGCGCT 1 cut(s) 675
Eco53kI GAGCTC 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 45
EcoICRI GAGCTC 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 478
EcoT14I CCWWGG 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 241
Esp3I CGTCTC 1 cut(s) 656
FaeI CATG 2 cut(s) 136, 569
FaqI GGGAC 2 cut(s) 63, 199
FatI CATG 2 cut(s) 132, 565
FauI CCCGC 1 cut(s) 776
FauNDI CATATG 1 cut(s) 550
FbaI TGATCA 1 cut(s) 259
FblI GTMKAC 1 cut(s) 434
Fnu4HI GCNGC 2 cut(s) 158, 762
FokI GGATG 2 cut(s) 210, 770
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 2 cut(s) 158, 762
GlaI GCGC 1 cut(s) 675
GluI GCNGC 2 cut(s) 158, 762
HaeII RGCGCY 1 cut(s) 677
HhaI GCGC 1 cut(s) 676
Hin1II CATG 2 cut(s) 136, 569
Hin6I GCGC 1 cut(s) 674
HinP1I GCGC 1 cut(s) 674
HincII GTYRAC 1 cut(s) 435
HindII GTYRAC 1 cut(s) 435
HindIII AAGCTT 1 cut(s) 471
HinfI GANTC 4 cut(s) 196, 274, 431, 555
HphI GGTGA 1 cut(s) 550
Hpy166II GTNNAC 3 cut(s) 353, 435, 539
Hpy188I TCNGA 3 cut(s) 89, 527, 778
Hpy188III TCNNGA 8 cut(s) 110, 118, 143, 200, 278, 389, 590, 698
Hpy8I GTNNAC 3 cut(s) 353, 435, 539
HpyAV CCTTC 2 cut(s) 651, 748
HpyCH4III ACNGT 1 cut(s) 350
HpyCH4IV ACGT 1 cut(s) 642
HpyCH4V TGCA 6 cut(s) 68, 160, 220, 313, 632, 722
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 23, 80, 135, 616
HpyF3I CTNAG 2 cut(s) 279, 670
HpySE526I ACGT 1 cut(s) 642
Hsp92II CATG 2 cut(s) 136, 569
HspAI GCGC 1 cut(s) 674
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 3 cut(s) 259, 586, 742
LguI GCTCTTC 1 cut(s) 390
LmnI GCTCC 2 cut(s) 302, 527
Lsp1109I GCAGC 2 cut(s) 144, 748
LweI GCATC 2 cut(s) 461, 748
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 2 cut(s) 19, 643
MalI GATC 3 cut(s) 261, 588, 744
MbiI CCGCTC 1 cut(s) 783
MboI GATC 3 cut(s) 259, 586, 742
MboII GAAGA 3 cut(s) 264, 377, 698
MfeI CAATTG 1 cut(s) 531
MhlI GDGCHC 3 cut(s) 131, 307, 409
MluCI AATT 3 cut(s) 186, 531, 728
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 1 cut(s) 490
MnlI CCTC 4 cut(s) 106, 320, 323, 571
MroXI GAANNNNTTC 2 cut(s) 602, 693
MseI TTAA 1 cut(s) 153
MspCI CTTAAG 1 cut(s) 152
MspR9I CCNGG 1 cut(s) 480
MunI CAATTG 1 cut(s) 531
Mva1269I GAATGC 1 cut(s) 575
MvaI CCWGG 1 cut(s) 480
MwoI GCNNNNNNNGC 5 cut(s) 14, 23, 80, 135, 616
NdeI CATATG 1 cut(s) 550
NdeII GATC 3 cut(s) 259, 586, 742
NlaIII CATG 2 cut(s) 136, 569
NlaIV GGNNCC 1 cut(s) 130
NmuCI GTSAC 1 cut(s) 19
PciSI GCTCTTC 1 cut(s) 390
PctI GAATGC 1 cut(s) 575
PdmI GAANNNNTTC 2 cut(s) 602, 693
PfeI GAWTC 3 cut(s) 196, 274, 555
PfoI TCCNGGA 1 cut(s) 478
PkrI GCNGC 2 cut(s) 159, 763
PleI GAGTC 1 cut(s) 439
PpsI GAGTC 1 cut(s) 439
PsiI TTATAA 1 cut(s) 738
Psp124BI GAGCTC 1 cut(s) 307
Psp6I CCWGG 1 cut(s) 478
PspGI CCWGG 1 cut(s) 478
PspN4I GGNNCC 1 cut(s) 130
PspPI GGNCC 3 cut(s) 77, 350, 700
PstI CTGCAG 2 cut(s) 70, 162
PstNI CAGNNNCTG 1 cut(s) 74
RsaI GTAC 1 cut(s) 540
RsaNI GTAC 1 cut(s) 539
SacI GAGCTC 1 cut(s) 307
SalI GTCGAC 1 cut(s) 433
SapI GCTCTTC 1 cut(s) 390
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 2 cut(s) 158, 762
Sau3AI GATC 3 cut(s) 259, 586, 742
Sau96I GGNCC 3 cut(s) 77, 350, 700
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 1 cut(s) 480
SduI GDGCHC 3 cut(s) 131, 307, 409
SetI ASST 9 cut(s) 85, 117, 140, 159, 232, 307, 475, 563, 645
SfaNI GCATC 2 cut(s) 461, 748
SfcI CTRYAG 2 cut(s) 66, 158
SinI GGWCC 3 cut(s) 77, 350, 700
SmlI CTYRAG 4 cut(s) 110, 118, 152, 428
SmoI CTYRAG 4 cut(s) 110, 118, 152, 428
Sse9I AATT 3 cut(s) 186, 531, 728
SsiI CCGC 2 cut(s) 8, 783
SspI AATATT 1 cut(s) 771
SstI GAGCTC 1 cut(s) 307
StyD4I CCNGG 1 cut(s) 478
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 1 cut(s) 350
TaiI ACGT 1 cut(s) 645
TaqI TCGA 1 cut(s) 434
TasI AATT 3 cut(s) 186, 531, 728
TfiI GAWTC 3 cut(s) 196, 274, 555
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TscAI CASTG 1 cut(s) 75
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 2 cut(s) 157, 761
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 2 cut(s) 179, 537
TspGWI ACGGA 2 cut(s) 556, 680
TspRI CASTG 1 cut(s) 75
Vha464I CTTAAG 1 cut(s) 152
VpaK11BI GGWCC 3 cut(s) 77, 350, 700
XapI RAATTY 1 cut(s) 186
XmiI GTMKAC 1 cut(s) 434
XmnI GAANNNNTTC 2 cut(s) 602, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.