MD11G1020800.v1.1

Biotin/lipoate A/B protein ligase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011903
Physical Location & Seq
Forward (+)
1808808 .. 1812573
3766 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1020800.v1.1.491

Sequence Viewer

Length: 741 bp
ATGCATAATTGGTTATACAGTGGTTTGCAGGAAATGAGGGTTTCGCGAAATCTTGAGGTCTTGAAAATGGGCACTATCAGCTATCTGGAAGCACTTAAGCTGCAGGAAAAGCTGGTAGCTGATAGAAAAAGTCATAAGATTCCAGATACCCTTGTGTCCCTGCAACATCCACCTACATATACCCTTGGCAAACGACTAACCGATCACAATCTGTTGATTCCGGAGACTGAACTCAAAAGAATAGGAGCTGAACTGCACTACACACAAAGAGGAGGAGACATTACATTTCATGGTCCACATCAAGCCATCTTGTATCCCATTATTGCTCTTCATGATATGGGGCTTGGTGCTCGTAAATCTGTGGAGAAACTTGAGTCGACTATGATTGACTTGGCTTCTCTGTATGGCGTGAAAGCTTGCCCTGGAAATAAATGTGAAACGGGGGTTTGGGTTGGAGAGAGAAAAATCGGAGCCATTGGTGTACAGATTTCGTATGGAGTCACCTCTCATGGGTTGGCATTCAACATGGATCCATATTTGAGCTACTTTAAGCAAATTGTGCCTTGTGGAATTGCAAATAAAGACGTTACATCATTGAGAAGGGAGACGGGCTTGGCGCTGCCTACGGAAGATGTAGTTCAGGACCAGTTGATTTCTTGTTTTGCAAGACAATTTGGTTATGGTGATCTTGTATGGAAGGATGCTGCGTCAGTATCGTCAGACAATGGGGAAACTGAATGA

Protein Analysis

247

Amino Acids

27.3

Weight (kDa)

6.5

Isoelectric Point (pI)

33.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 28 - 214 8.6e-40 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011903)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04640 AT1G04640
fragaria_vesca FvH4_3g43830
malus_domestica MD11G1020800.v1.1
prunus_persica Prupe.6G017700_v2.0.a1
pyrus_communis pycom03g01630
rosa_chinensis RchiOBHm_Chr5g0079311
rosa_laevigata RLG00000036810
rosa_multiflora Rmu_sc0000607.1_g000005 Rmu_sc0002187.1_g000033
rosa_roxburghii Rroxscaffold_1G00003400 Rroxscaffold_1G00003620
rosa_rugosa Rorug05G0464300
rosa_samantha Rh5AG515200 Rh5BG540100 Rh5CG563100 Rh5DG550500
rosa_wichuraiana Rw0G005210 Rw5G048050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 377
AccII CGCG 1 cut(s) 46
AccIII TCCGGA 1 cut(s) 220
AclWI GGATC 2 cut(s) 524, 537
AfaI GTAC 1 cut(s) 483
AfiI CCNNNNNNNGG 1 cut(s) 510
AflII CTTAAG 1 cut(s) 95
AgsI TTSAA 2 cut(s) 64, 523
AjnI CCWGG 1 cut(s) 421
AluBI AGCT 7 cut(s) 81, 100, 112, 119, 248, 416, 543
AluI AGCT 7 cut(s) 81, 100, 112, 119, 248, 416, 543
Alw21I GWGCWC 1 cut(s) 352
Alw26I GTCTC 3 cut(s) 218, 270, 599
AlwI GGATC 2 cut(s) 524, 537
Aor13HI TCCGGA 1 cut(s) 220
ApeKI GCWGC 3 cut(s) 100, 619, 704
AspLEI GCGC 1 cut(s) 619
AspS9I GGNCC 2 cut(s) 293, 643
AsuHPI GGTGA 2 cut(s) 493, 695
AvaII GGWCC 2 cut(s) 293, 643
BaeGI GKGCMC 1 cut(s) 74
BaeI ACNNNNGTAYC 2 cut(s) 138, 171
BamHI GGATCC 1 cut(s) 529
Bbv12I GWGCWC 1 cut(s) 352
BbvI GCAGC 3 cut(s) 87, 606, 691
BccI CCATC 1 cut(s) 314
BciT130I CCWGG 1 cut(s) 423
BciVI GTATCC 1 cut(s) 324
BcoDI GTCTC 3 cut(s) 218, 270, 599
BfmI CTRYAG 1 cut(s) 101
BfoI RGCGCY 1 cut(s) 620
BfrI CTTAAG 1 cut(s) 95
BfuI GTATCC 1 cut(s) 324
BisI GCNGC 3 cut(s) 101, 620, 705
BlsI GCNGC 3 cut(s) 102, 621, 706
Bme1390I CCNGG 1 cut(s) 423
Bme18I GGWCC 2 cut(s) 293, 643
BmgT120I GGNCC 2 cut(s) 293, 643
BmiI GGNNCC 2 cut(s) 472, 531
BmrFI CCNGG 1 cut(s) 423
BmsI GCATC 1 cut(s) 691
BpuEI CTTGAG 2 cut(s) 74, 392
BsaBI GATNNNNATC 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 184, 421
BsaWI WCCGGW 1 cut(s) 220
Bsc4I CCNNNNNNNGG 1 cut(s) 510
Bse1I ACTGG 1 cut(s) 646
Bse8I GATNNNNATC 1 cut(s) 207
BseAI TCCGGA 1 cut(s) 220
BseBI CCWGG 1 cut(s) 423
BseDI CCNNGG 2 cut(s) 184, 421
BseGI GGATG 2 cut(s) 166, 706
BseJI GATNNNNATC 1 cut(s) 207
BseLI CCNNNNNNNGG 1 cut(s) 510
BseNI ACTGG 1 cut(s) 646
BseRI GAGGAG 2 cut(s) 285, 288
BseSI GKGCMC 1 cut(s) 74
BseXI GCAGC 3 cut(s) 87, 606, 691
BsgI GTGCAG 1 cut(s) 239
Bsh1236I CGCG 1 cut(s) 46
BsiHKAI GWGCWC 1 cut(s) 352
BsiSI CCGG 1 cut(s) 221
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 1 cut(s) 510
BsmAI GTCTC 3 cut(s) 218, 270, 599
BsmBI CGTCTC 1 cut(s) 599
BsmFI GGGAC 1 cut(s) 142
BsmI GAATGC 1 cut(s) 518
Bsp1286I GDGCHC 2 cut(s) 74, 352
Bsp13I TCCGGA 1 cut(s) 220
Bsp1407I TGTACA 1 cut(s) 481
Bsp143I GATC 3 cut(s) 202, 529, 685
Bsp68I TCGCGA 1 cut(s) 46
BspEI TCCGGA 1 cut(s) 220
BspFNI CGCG 1 cut(s) 46
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 2 cut(s) 472, 531
BspMAI CTGCAG 1 cut(s) 105
BspPI GGATC 2 cut(s) 524, 537
BspQI GCTCTTC 1 cut(s) 333
BspTI CTTAAG 1 cut(s) 95
BsrGI TGTACA 1 cut(s) 481
BsrI ACTGG 1 cut(s) 646
BssECI CCNNGG 2 cut(s) 184, 421
BssMI GATC 3 cut(s) 202, 529, 685
BssT1I CCWWGG 1 cut(s) 184
Bst2UI CCWGG 1 cut(s) 423
Bst4CI ACNGT 1 cut(s) 20
Bst6I CTCTTC 1 cut(s) 333
BstAFI CTTAAG 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 559
BstAUI TGTACA 1 cut(s) 481
BstC8I GCNNGC 1 cut(s) 418
BstF5I GGATG 2 cut(s) 166, 706
BstFNI CGCG 1 cut(s) 46
BstH2I RGCGCY 1 cut(s) 620
BstHHI GCGC 1 cut(s) 619
BstKTI GATC 3 cut(s) 205, 532, 688
BstMAI GTCTC 3 cut(s) 218, 270, 599
BstMBI GATC 3 cut(s) 202, 529, 685
BstMWI GCNNNNNNNGC 3 cut(s) 78, 109, 559
BstNI CCWGG 1 cut(s) 423
BstSCI CCNGG 1 cut(s) 421
BstSFI CTRYAG 1 cut(s) 101
BstSLI GKGCMC 1 cut(s) 74
BstUI CGCG 1 cut(s) 46
BstV1I GCAGC 3 cut(s) 87, 606, 691
BstX2I RGATCY 1 cut(s) 529
BstYI RGATCY 1 cut(s) 529
BsuI GTATCC 1 cut(s) 324
BtsCI GGATG 2 cut(s) 166, 706
BtsIMutI CAGTG 1 cut(s) 25
BtuMI TCGCGA 1 cut(s) 46
Cac8I GCNNGC 1 cut(s) 418
CciI TCATGA 1 cut(s) 331
CfoI GCGC 1 cut(s) 619
Cfr13I GGNCC 2 cut(s) 293, 643
CseI GACGC 1 cut(s) 696
Csp6I GTAC 1 cut(s) 482
CviAII CATG 4 cut(s) 290, 332, 509, 526
CviQI GTAC 1 cut(s) 482
DpnI GATC 3 cut(s) 204, 531, 687
DpnII GATC 3 cut(s) 202, 529, 685
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
Eco130I CCWWGG 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 293, 643
EcoRII CCWGG 1 cut(s) 421
EcoT14I CCWWGG 1 cut(s) 184
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 184
Esp3I CGTCTC 1 cut(s) 599
FaeI CATG 4 cut(s) 293, 335, 512, 529
FaqI GGGAC 1 cut(s) 142
FatI CATG 4 cut(s) 289, 331, 508, 525
FblI GTMKAC 1 cut(s) 377
Fnu4HI GCNGC 3 cut(s) 101, 620, 705
FokI GGATG 2 cut(s) 153, 713
Fsp4HI GCNGC 3 cut(s) 101, 620, 705
GlaI GCGC 1 cut(s) 618
GluI GCNGC 3 cut(s) 101, 620, 705
HaeII RGCGCY 1 cut(s) 620
HapII CCGG 1 cut(s) 221
HgaI GACGC 1 cut(s) 696
HhaI GCGC 1 cut(s) 619
Hin1II CATG 4 cut(s) 293, 335, 512, 529
Hin6I GCGC 1 cut(s) 617
HinP1I GCGC 1 cut(s) 617
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HindIII AAGCTT 1 cut(s) 414
HinfI GANTC 4 cut(s) 139, 217, 374, 498
HpaII CCGG 1 cut(s) 221
HphI GGTGA 2 cut(s) 493, 695
Hpy166II GTNNAC 3 cut(s) 296, 378, 482
Hpy188I TCNGA 2 cut(s) 470, 721
Hpy188III TCNNGA 8 cut(s) 45, 53, 61, 86, 143, 221, 332, 641
Hpy8I GTNNAC 3 cut(s) 296, 378, 482
HpyAV CCTTC 2 cut(s) 594, 691
HpyCH4III ACNGT 1 cut(s) 20
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 7 cut(s) 4, 28, 103, 163, 256, 575, 665
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 109, 559
HpySE526I ACGT 1 cut(s) 585
Hsp92II CATG 4 cut(s) 293, 335, 512, 529
HspAI GCGC 1 cut(s) 617
Kpn2I TCCGGA 1 cut(s) 220
Kzo9I GATC 3 cut(s) 202, 529, 685
LguI GCTCTTC 1 cut(s) 333
LmnI GCTCC 2 cut(s) 245, 470
Lsp1109I GCAGC 3 cut(s) 87, 606, 691
LweI GCATC 1 cut(s) 691
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 2 cut(s) 499, 586
MalI GATC 3 cut(s) 204, 531, 687
MboI GATC 3 cut(s) 202, 529, 685
MboII GAAGA 2 cut(s) 320, 641
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 2 cut(s) 74, 352
MluCI AATT 4 cut(s) 7, 555, 570, 671
MlyI GAGTC 2 cut(s) 383, 507
MmeI TCCRAC 1 cut(s) 433
MnlI CCTC 5 cut(s) 30, 49, 263, 266, 514
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 220
MseI TTAA 2 cut(s) 96, 549
MspCI CTTAAG 1 cut(s) 95
MspI CCGG 1 cut(s) 221
MspR9I CCNGG 1 cut(s) 423
Mva1269I GAATGC 1 cut(s) 518
MvaI CCWGG 1 cut(s) 423
MvnI CGCG 1 cut(s) 46
MwoI GCNNNNNNNGC 3 cut(s) 78, 109, 559
NdeII GATC 3 cut(s) 202, 529, 685
NlaIII CATG 4 cut(s) 293, 335, 512, 529
NlaIV GGNNCC 2 cut(s) 472, 531
NmuCI GTSAC 1 cut(s) 499
NruI TCGCGA 1 cut(s) 46
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 331
PciSI GCTCTTC 1 cut(s) 333
PctI GAATGC 1 cut(s) 518
PfeI GAWTC 2 cut(s) 139, 217
PkrI GCNGC 3 cut(s) 102, 621, 706
PleI GAGTC 2 cut(s) 382, 506
PpsI GAGTC 2 cut(s) 382, 506
Psp6I CCWGG 1 cut(s) 421
PspGI CCWGG 1 cut(s) 421
PspN4I GGNNCC 2 cut(s) 472, 531
PspPI GGNCC 2 cut(s) 293, 643
PstI CTGCAG 1 cut(s) 105
PsuI RGATCY 1 cut(s) 529
RruI TCGCGA 1 cut(s) 46
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
SalI GTCGAC 1 cut(s) 376
SapI GCTCTTC 1 cut(s) 333
SaqAI TTAA 2 cut(s) 96, 549
SatI GCNGC 3 cut(s) 101, 620, 705
Sau3AI GATC 3 cut(s) 202, 529, 685
Sau96I GGNCC 2 cut(s) 293, 643
SchI GAGTC 2 cut(s) 383, 507
ScrFI CCNGG 1 cut(s) 423
SduI GDGCHC 2 cut(s) 74, 352
SfaNI GCATC 1 cut(s) 691
SfcI CTRYAG 1 cut(s) 101
SinI GGWCC 2 cut(s) 293, 643
SmlI CTYRAG 3 cut(s) 53, 95, 371
SmoI CTYRAG 3 cut(s) 53, 95, 371
Sse9I AATT 4 cut(s) 7, 555, 570, 671
StyD4I CCNGG 1 cut(s) 421
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 1 cut(s) 20
TaiI ACGT 1 cut(s) 588
TaqI TCGA 1 cut(s) 377
TasI AATT 4 cut(s) 7, 555, 570, 671
TatI WGTACW 1 cut(s) 481
TfiI GAWTC 2 cut(s) 139, 217
Tru1I TTAA 2 cut(s) 96, 549
Tru9I TTAA 2 cut(s) 96, 549
TscAI CASTG 1 cut(s) 25
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 3 cut(s) 100, 619, 704
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 2 cut(s) 278, 320
TspGWI ACGGA 1 cut(s) 641
TspRI CASTG 1 cut(s) 25
Vha464I CTTAAG 1 cut(s) 95
VpaK11BI GGWCC 2 cut(s) 293, 643
XmiI GTMKAC 1 cut(s) 377
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.