FvH4_3g43830

Biotin/lipoate A/B protein ligase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011903
Physical Location & Seq
Reverse (-)
36509926 .. 36511320
1395 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g43830.t1

Sequence Viewer

Length: 714 bp
ATGAGAGTTCCGCGGACTCTTGAGGTTTGGAAGCTGGGGACTGTCAACTATCTGCAGGCACTTAAGCTGCAAGAGAAGCTGGTTTCGGACAGAAAGGTTCATAATATTCCGGATACTCTCCTGGCCCTGCAGCATCCGCCGACATACACACTTGGAAAGCGGCGAACTGATCATAATCTATTAGTTTCTGAGGCTGAACTGAAAGGAATAGGTGCTGAACTGCACTACACGCAAAGAGGAGGAGACATTACGTTTCATGGTCCAAGACAGGCCATTTTGTATCCCATTGTTTCGCTTAGAGATATTGGGTTTGGGGCTCGGAGATATGTGGAGACGCTTGAGTTGACTATGATTGAATTGGCGTCTCAGTATGGCGTGAAAGCTTGTCCGGGGCAAGCAGGAGAGACTGGGGTGTGGGTGGGAGAGAGAAAGATTGGTGCAATTGGTGTGCGCATTTCATATGGAGTTACCTCTCATGGGTTGGCATTCAACATTGATCCAGATTTAAACTATTTCAAGCACATTGTGCCATGTGGTATTGCTGATAAAGAAGTGACATCTCTGAGAAAGGAGACAGACTTGGCGCTTCCTACCGATGAAGTAGTTCACGACCAGTTGATTTCTTGCTTTGCAAGATTGTTTGGTTATGGTAACCTTGTATGGAAGGATGCAGATGCTGCTTCAATATTATCAGACTATGGGGAAATCAACTGA

Protein Analysis

238

Amino Acids

26.31

Weight (kDa)

6.45

Isoelectric Point (pI)

28.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 16 - 203 3.5e-43 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011903)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04640 AT1G04640
fragaria_vesca FvH4_3g43830
malus_domestica MD11G1020800.v1.1
prunus_persica Prupe.6G017700_v2.0.a1
pyrus_communis pycom03g01630
rosa_chinensis RchiOBHm_Chr5g0079311
rosa_laevigata RLG00000036810
rosa_multiflora Rmu_sc0000607.1_g000005 Rmu_sc0002187.1_g000033
rosa_roxburghii Rroxscaffold_1G00003400 Rroxscaffold_1G00003620
rosa_rugosa Rorug05G0464300
rosa_samantha Rh5AG515200 Rh5BG540100 Rh5CG563100 Rh5DG550500
rosa_wichuraiana Rw0G005210 Rw5G048050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 452
AccII CGCG 1 cut(s) 13
AccIII TCCGGA 1 cut(s) 109
AciI CCGC 4 cut(s) 11, 13, 137, 160
AclWI GGATC 1 cut(s) 491
AcyI GRCGYC 1 cut(s) 362
AdeI CACNNNGTG 1 cut(s) 526
AfiI CCNNNNNNNGG 1 cut(s) 477
AflII CTTAAG 1 cut(s) 62
AgsI TTSAA 4 cut(s) 356, 490, 517, 684
AjnI CCWGG 1 cut(s) 120
AluBI AGCT 4 cut(s) 34, 67, 79, 383
AluI AGCT 4 cut(s) 34, 67, 79, 383
Alw26I GTCTC 5 cut(s) 237, 326, 369, 398, 566
AlwI GGATC 1 cut(s) 491
AlwNI CAGNNNCTG 1 cut(s) 677
Aor13HI TCCGGA 1 cut(s) 109
AoxI GGCC 2 cut(s) 123, 270
ApeKI GCWGC 3 cut(s) 67, 130, 677
Asp700I GAANNNNTTC 1 cut(s) 603
AspLEI GCGC 2 cut(s) 453, 586
AspS9I GGNCC 2 cut(s) 124, 260
AsuC2I CCSGG 1 cut(s) 390
AvaII GGWCC 1 cut(s) 260
BanII GRGCYC 1 cut(s) 319
BbvI GCAGC 3 cut(s) 54, 142, 664
BciT130I CCWGG 1 cut(s) 122
BciVI GTATCC 2 cut(s) 106, 291
BclI TGATCA 1 cut(s) 169
BcnI CCSGG 1 cut(s) 390
BcoDI GTCTC 5 cut(s) 237, 326, 369, 398, 566
BfmI CTRYAG 2 cut(s) 53, 128
BfoI RGCGCY 1 cut(s) 587
BfrI CTTAAG 1 cut(s) 62
BfuI GTATCC 2 cut(s) 106, 291
BisI GCNGC 4 cut(s) 68, 131, 161, 678
BlsI GCNGC 4 cut(s) 69, 132, 162, 679
Bme1390I CCNGG 2 cut(s) 122, 390
Bme18I GGWCC 1 cut(s) 260
BmgT120I GGNCC 2 cut(s) 124, 260
BmrFI CCNGG 2 cut(s) 122, 390
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 3 cut(s) 142, 658, 664
BmuI ACTGGG 1 cut(s) 417
BpuEI CTTGAG 2 cut(s) 41, 359
BpuMI CCSGG 1 cut(s) 390
BsaBI GATNNNNATC 1 cut(s) 174
BsaHI GRCGYC 1 cut(s) 362
BsaJI CCNNGG 2 cut(s) 11, 389
BsaWI WCCGGW 1 cut(s) 109
Bsc4I CCNNNNNNNGG 1 cut(s) 477
Bse1I ACTGG 2 cut(s) 412, 613
Bse8I GATNNNNATC 1 cut(s) 174
BseAI TCCGGA 1 cut(s) 109
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 2 cut(s) 11, 389
BseGI GGATG 2 cut(s) 133, 673
BseJI GATNNNNATC 1 cut(s) 174
BseLI CCNNNNNNNGG 1 cut(s) 477
BseMII CTCAG 3 cut(s) 180, 380, 554
BseNI ACTGG 2 cut(s) 412, 613
BseRI GAGGAG 2 cut(s) 252, 255
BseXI GCAGC 3 cut(s) 54, 142, 664
BseYI CCCAGC 1 cut(s) 34
BsgI GTGCAG 1 cut(s) 206
Bsh1236I CGCG 1 cut(s) 13
BshFI GGCC 2 cut(s) 125, 272
BsiSI CCGG 2 cut(s) 110, 389
BslFI GGGAC 1 cut(s) 52
BslI CCNNNNNNNGG 1 cut(s) 477
BsmAI GTCTC 5 cut(s) 237, 326, 369, 398, 566
BsmBI CGTCTC 2 cut(s) 326, 369
BsmFI GGGAC 1 cut(s) 52
BsmI GAATGC 1 cut(s) 485
BsnI GGCC 2 cut(s) 125, 272
Bsp1286I GDGCHC 1 cut(s) 319
Bsp13I TCCGGA 1 cut(s) 109
Bsp143I GATC 2 cut(s) 169, 496
BspACI CCGC 4 cut(s) 11, 13, 137, 160
BspANI GGCC 2 cut(s) 125, 272
BspCNI CTCAG 3 cut(s) 181, 379, 555
BspEI TCCGGA 1 cut(s) 109
BspFNI CGCG 1 cut(s) 13
BspMAI CTGCAG 2 cut(s) 57, 132
BspPI GGATC 1 cut(s) 491
BspTI CTTAAG 1 cut(s) 62
BsrI ACTGG 2 cut(s) 412, 613
BssECI CCNNGG 2 cut(s) 11, 389
BssMI GATC 2 cut(s) 169, 496
BssNI GRCGYC 1 cut(s) 362
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 1 cut(s) 43
BstACI GRCGYC 1 cut(s) 362
BstAFI CTTAAG 1 cut(s) 62
BstAPI GCANNNNNTGC 2 cut(s) 526, 677
BstC8I GCNNGC 2 cut(s) 57, 396
BstDEI CTNAG 4 cut(s) 189, 296, 366, 563
BstDSI CCRYGG 1 cut(s) 11
BstEII GGTNACC 1 cut(s) 650
BstF5I GGATG 2 cut(s) 133, 673
BstFNI CGCG 1 cut(s) 13
BstH2I RGCGCY 1 cut(s) 587
BstHHI GCGC 2 cut(s) 453, 586
BstKTI GATC 2 cut(s) 172, 499
BstMAI GTCTC 5 cut(s) 237, 326, 369, 398, 566
BstMBI GATC 2 cut(s) 169, 496
BstMWI GCNNNNNNNGC 5 cut(s) 76, 136, 229, 526, 677
BstNI CCWGG 1 cut(s) 122
BstPI GGTNACC 1 cut(s) 650
BstSCI CCNGG 2 cut(s) 120, 388
BstSFI CTRYAG 2 cut(s) 53, 128
BstUI CGCG 1 cut(s) 13
BstV1I GCAGC 3 cut(s) 54, 142, 664
BsuI GTATCC 2 cut(s) 106, 291
BsuRI GGCC 2 cut(s) 125, 272
BtgI CCRYGG 1 cut(s) 11
BtsCI GGATG 2 cut(s) 133, 673
Cac8I GCNNGC 2 cut(s) 57, 396
CaiI CAGNNNCTG 1 cut(s) 677
CfoI GCGC 2 cut(s) 453, 586
Cfr13I GGNCC 2 cut(s) 124, 260
Cfr42I CCGCGG 1 cut(s) 14
CseI GACGC 2 cut(s) 343, 351
CviAII CATG 3 cut(s) 257, 476, 531
CviJI RGCY 8 cut(s) 34, 67, 79, 125, 194, 272, 317, 383
CviKI_1 RGCY 8 cut(s) 34, 67, 79, 125, 194, 272, 317, 383
DdeI CTNAG 4 cut(s) 189, 296, 366, 563
DpnI GATC 2 cut(s) 171, 498
DpnII GATC 2 cut(s) 169, 496
DraI TTTAAA 1 cut(s) 507
DraIII CACNNNGTG 1 cut(s) 526
EciI GGCGGA 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 260
Eco91I GGTNACC 1 cut(s) 650
EcoO65I GGTNACC 1 cut(s) 650
EcoRII CCWGG 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 319
Esp3I CGTCTC 2 cut(s) 326, 369
FaeI CATG 3 cut(s) 260, 479, 534
FaqI GGGAC 1 cut(s) 52
FatI CATG 3 cut(s) 256, 475, 530
FauNDI CATATG 1 cut(s) 460
FbaI TGATCA 1 cut(s) 169
Fnu4HI GCNGC 4 cut(s) 68, 131, 161, 678
FokI GGATG 2 cut(s) 120, 680
FriOI GRGCYC 1 cut(s) 319
Fsp4HI GCNGC 4 cut(s) 68, 131, 161, 678
FspAI RTGCGCAY 1 cut(s) 452
FspI TGCGCA 1 cut(s) 452
GlaI GCGC 2 cut(s) 452, 585
GluI GCNGC 4 cut(s) 68, 131, 161, 678
GsaI CCCAGC 1 cut(s) 38
HaeII RGCGCY 1 cut(s) 587
HaeIII GGCC 2 cut(s) 125, 272
HapII CCGG 2 cut(s) 110, 389
HgaI GACGC 2 cut(s) 343, 351
HhaI GCGC 2 cut(s) 453, 586
Hin1I GRCGYC 1 cut(s) 362
Hin1II CATG 3 cut(s) 260, 479, 534
Hin6I GCGC 2 cut(s) 451, 584
HinP1I GCGC 2 cut(s) 451, 584
HincII GTYRAC 2 cut(s) 46, 345
HindII GTYRAC 2 cut(s) 46, 345
HindIII AAGCTT 1 cut(s) 381
HinfI GANTC 1 cut(s) 16
HpaII CCGG 2 cut(s) 110, 389
Hpy166II GTNNAC 3 cut(s) 46, 345, 607
Hpy188I TCNGA 5 cut(s) 88, 190, 321, 564, 694
Hpy188III TCNNGA 4 cut(s) 20, 110, 500, 608
Hpy8I GTNNAC 3 cut(s) 46, 345, 607
HpyAV CCTTC 1 cut(s) 658
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4IV ACGT 1 cut(s) 251
HpyCH4V TGCA 7 cut(s) 55, 70, 130, 223, 440, 632, 671
HpyF10VI GCNNNNNNNGC 5 cut(s) 76, 136, 229, 526, 677
HpyF3I CTNAG 4 cut(s) 189, 296, 366, 563
HpySE526I ACGT 1 cut(s) 251
Hsp92I GRCGYC 1 cut(s) 362
Hsp92II CATG 3 cut(s) 260, 479, 534
HspAI GCGC 2 cut(s) 451, 584
Kpn2I TCCGGA 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 169
KspI CCGCGG 1 cut(s) 14
Kzo9I GATC 2 cut(s) 169, 496
Lsp1109I GCAGC 3 cut(s) 54, 142, 664
LweI GCATC 3 cut(s) 142, 658, 664
MaeII ACGT 1 cut(s) 251
MaeIII GTNAC 3 cut(s) 466, 553, 650
MalI GATC 2 cut(s) 171, 498
MboI GATC 2 cut(s) 169, 496
MfeI CAATTG 1 cut(s) 441
MhlI GDGCHC 1 cut(s) 319
MluCI AATT 2 cut(s) 356, 441
MlyI GAGTC 1 cut(s) 10
MnlI CCTC 5 cut(s) 16, 184, 230, 233, 481
MroI TCCGGA 1 cut(s) 109
MroXI GAANNNNTTC 1 cut(s) 603
MseI TTAA 2 cut(s) 63, 506
MspA1I CMGCKG 1 cut(s) 13
MspCI CTTAAG 1 cut(s) 62
MspI CCGG 2 cut(s) 110, 389
MspR9I CCNGG 2 cut(s) 122, 390
MunI CAATTG 1 cut(s) 441
Mva1269I GAATGC 1 cut(s) 485
MvaI CCWGG 1 cut(s) 122
MvnI CGCG 1 cut(s) 13
MwoI GCNNNNNNNGC 5 cut(s) 76, 136, 229, 526, 677
NciI CCSGG 1 cut(s) 390
NdeI CATATG 1 cut(s) 460
NdeII GATC 2 cut(s) 169, 496
NlaIII CATG 3 cut(s) 260, 479, 534
NmuCI GTSAC 1 cut(s) 553
NsbI TGCGCA 1 cut(s) 452
PctI GAATGC 1 cut(s) 485
PdmI GAANNNNTTC 1 cut(s) 603
PkrI GCNGC 4 cut(s) 69, 132, 162, 679
PleI GAGTC 1 cut(s) 10
PpsI GAGTC 1 cut(s) 10
Psp6I CCWGG 1 cut(s) 120
PspEI GGTNACC 1 cut(s) 650
PspFI CCCAGC 1 cut(s) 34
PspGI CCWGG 1 cut(s) 120
PspPI GGNCC 2 cut(s) 124, 260
PstI CTGCAG 2 cut(s) 57, 132
PstNI CAGNNNCTG 1 cut(s) 677
SacII CCGCGG 1 cut(s) 14
SaqAI TTAA 2 cut(s) 63, 506
SatI GCNGC 4 cut(s) 68, 131, 161, 678
Sau3AI GATC 2 cut(s) 169, 496
Sau96I GGNCC 2 cut(s) 124, 260
SchI GAGTC 1 cut(s) 10
ScrFI CCNGG 2 cut(s) 122, 390
SduI GDGCHC 1 cut(s) 319
SfaNI GCATC 3 cut(s) 142, 658, 664
SfcI CTRYAG 2 cut(s) 53, 128
Sfr303I CCGCGG 1 cut(s) 14
SgrBI CCGCGG 1 cut(s) 14
SinI GGWCC 1 cut(s) 260
SmlI CTYRAG 3 cut(s) 20, 62, 338
SmoI CTYRAG 3 cut(s) 20, 62, 338
Sse9I AATT 2 cut(s) 356, 441
SsiI CCGC 4 cut(s) 11, 13, 137, 160
SspI AATATT 2 cut(s) 106, 687
StyD4I CCNGG 2 cut(s) 120, 388
TaaI ACNGT 1 cut(s) 43
TaiI ACGT 1 cut(s) 254
TasI AATT 2 cut(s) 356, 441
TauI GCSGC 1 cut(s) 163
Tru1I TTAA 2 cut(s) 63, 506
Tru9I TTAA 2 cut(s) 63, 506
TseFI GTSAC 1 cut(s) 553
TseI GCWGC 3 cut(s) 67, 130, 677
Tsp45I GTSAC 1 cut(s) 553
TspDTI ATGAA 4 cut(s) 89, 245, 447, 612
Vha464I CTTAAG 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 260
XmnI GAANNNNTTC 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.