AT1G05600

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010477
Physical Location & Seq
Forward (+)
1671916 .. 1673923
2008 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G05600.2

Sequence Viewer

Length: 1515 bp
ATGAGTGTTGTTAGATGGCCAAGAGTTCTAACGCCAAGTCTTCTTTCTCAAATCCTAAAGAAGCAGAAGAATCCAGTGACAGCACTTAAGCTTTTTGAGGAAGCGAAAGAGAGGTTTCCGAGTTACGGTCACAATGGCTCGGTGTATGCTACCATGATTGACATTCTCGGTAAATCTAACCGTGTGTTGGAAATGAAATATGTCATTGAGAGAATGAAGGAAGATTCTTGTGAATGTAAAGATTCGGTTTTTGCATCGGTTATTCGTACATTCTCCAGAGCTGGAAGGCTGGAAGATGCTATTTCTCTCTTCAAGAGCCTTCATGAGTTCAATTGTGTGAATTGGAGTCTATCTTTCGATACTCTTTTACAGGAGATGGTTAAAGAATCCGAGCTCGAAGCTGCTTGTCATATCTTCAGAAAGTATTGTTATGGTTGGGAGGTGAATTCTCGAATAACGGCTCTGAATTTGCTTATGAAGGTTCTTTGCCAGGTCAATCGCTCTGACCTTGCGTCTCAGGTTTTTCAAGAGATGAATTACCAGGGTTGTTATCCGGACAGAGACAGTTACAGGATTTTGATGAAGGGGTTTTGTCTAGAGGGGAAACTTGAGGAAGCCACTCACCTGTTGTATTCAATGTTCTGGAGGATCTCACAGAAAGGTTCAGGTGAGGACATTGTGGTTTATAGAATTCTATTGGATGCATTATGTGATGCTGGTGAGGTTGATGATGCTATTGAAATTCTTGGTAAAATCTTGAGAAAAGGATTAAAGGCTCCGAAACGATGTTACCATCATATTGAAGCTGGTCACTGGGAGAGTAGCAGTGAGGGTATAGAGCGGGTGAAACGATTGTTAACCGAGACTTTGATCCGAGGTGCAATTCCCTGTTTGGATAGCTATAGTGCCATGGCTACTGATCTTTTTGAGGAAGGTAAGCTTGTTGAAGGTGAAGAGGTGCTCCTTGCAATGCGAAGCAAAGGCTTTGAGCCTACACCATTTATTTATGGTGCCAAGGTTAAAGCACTCTGTAGAGCCGGGAAGCTTAAAGAAGCAGTTTCTGTAATCAATAAGGAGATGATGCAAGGTCATTGCCTTCCAACAGTTGGAGTGTATAACGTTCTTATAAAAGGTCTATGCGATGATGGAAAATCCATGGAGGCTGTTGGATATCTCAAAAAGATGTCTAAGCAAGTTTCTTGTGTAGCGAACGAAGAAACATATCAAACTCTAGTGGATGGTCTGTGTCGAGATGGTCAATTTCTTGAGGCGAGTCAGGTCATGGAGGAGATGTTGATAAAATCACACTTTCCTGGTGTCGAAACTTACCATATGATGATAAAAGGTCTTTGTGATATGGACCGGCGATATGAAGCTGTGATGTGGTTGGAAGAGATGGTTAGTCAGGATATGGTACCGGAATCTTCTGTGTGGAAAGCTTTGGCTGAGTCTGTCTGCTTTTGTGCCATTGATGTTGTTGAGATACTTGAGCATCTAATTAGTAGCAAACGTTAA

Protein Analysis

504

Amino Acids

57.27

Weight (kDa)

5.89

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 154 - 198 2e-10 PPR repeat family
PPR_1 PF12854 182 - 212 2.2e-06 PPR repeat
PPR_2 PF13041 340 - 381 4e-06 PPR repeat family
PPR_1 PF12854 365 - 395 5.9e-06 PPR repeat
PPR_2 PF13041 367 - 417 1e-09 PPR repeat family
PPR_1 PF12854 401 - 431 5.5e-08 PPR repeat
PPR_2 PF13041 404 - 452 2.3e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1127
Acc65I GGTACC 1 cut(s) 1414
AccB1I GGYRCC 2 cut(s) 1010, 1414
AccB7I CCANNNNNTGG 1 cut(s) 1106
AccBSI CCGCTC 1 cut(s) 841
AccIII TCCGGA 1 cut(s) 553
AciI CCGC 1 cut(s) 841
AclI AACGTT 2 cut(s) 1119, 1510
AclWI GGATC 2 cut(s) 656, 865
AcoI YGGCCR 1 cut(s) 17
AcsI RAATTY 4 cut(s) 445, 466, 690, 741
AcuI CTGAAG 1 cut(s) 400
AfaI GTAC 2 cut(s) 268, 1416
AfiI CCNNNNNNNGG 3 cut(s) 125, 187, 1106
AflII CTTAAG 1 cut(s) 86
AgsI TTSAA 7 cut(s) 313, 331, 527, 636, 740, 803, 947
AhdI GACNNNNNGTC 1 cut(s) 511
AjnI CCWGG 3 cut(s) 489, 540, 1312
AjuI GAANNNNNNNTTGG 2 cut(s) 28, 60
Alw21I GWGCWC 2 cut(s) 396, 963
Alw26I GTCTC 3 cut(s) 519, 555, 857
AlwI GGATC 2 cut(s) 656, 865
AlwNI CAGNNNCTG 1 cut(s) 1061
Aor13HI TCCGGA 1 cut(s) 553
AoxI GGCC 1 cut(s) 17
ApeKI GCWGC 1 cut(s) 401
ApoI RAATTY 4 cut(s) 445, 466, 690, 741
Asp718I GGTACC 1 cut(s) 1414
AspS9I GGNCC 1 cut(s) 1360
AsuC2I CCSGG 1 cut(s) 1039
AsuHPI GGTGA 6 cut(s) 454, 614, 680, 731, 856, 962
AvaII GGWCC 1 cut(s) 1360
BalI TGGCCA 1 cut(s) 19
BanI GGYRCC 2 cut(s) 1010, 1414
BanII GRGCYC 1 cut(s) 396
BbsI GAAGAC 1 cut(s) 32
Bbv12I GWGCWC 2 cut(s) 396, 963
BbvI GCAGC 1 cut(s) 388
BccI CCATC 7 cut(s) 9, 370, 801, 1139, 1232, 1247, 1390
BceAI ACGGC 1 cut(s) 474
BciT130I CCWGG 3 cut(s) 491, 542, 1314
BcnI CCSGG 1 cut(s) 1039
BcoDI GTCTC 3 cut(s) 519, 555, 857
BfaI CTAG 2 cut(s) 596, 1232
BfmI CTRYAG 2 cut(s) 901, 1030
BfrI CTTAAG 1 cut(s) 86
BisI GCNGC 1 cut(s) 402
BlsI GCNGC 1 cut(s) 403
Bme1390I CCNGG 4 cut(s) 491, 542, 1039, 1314
Bme18I GGWCC 1 cut(s) 1360
BmeRI GACNNNNNGTC 1 cut(s) 511
BmgT120I GGNCC 1 cut(s) 1360
BmiI GGNNCC 3 cut(s) 777, 1012, 1416
BmrFI CCNGG 4 cut(s) 491, 542, 1039, 1314
BmrI ACTGGG 1 cut(s) 823
BmsI GCATC 7 cut(s) 263, 286, 691, 703, 721, 1071, 1501
BmuI ACTGGG 1 cut(s) 823
BpiI GAAGAC 1 cut(s) 32
BpmI CTGGAG 2 cut(s) 259, 664
BpuEI CTTGAG 4 cut(s) 629, 778, 1286, 1508
BpuMI CCSGG 1 cut(s) 1039
BsaJI CCNNGG 5 cut(s) 541, 874, 909, 1014, 1155
BsaWI WCCGGW 2 cut(s) 553, 1417
BsaXI ACNNNNNCTCC 2 cut(s) 1277, 1307
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 187, 1106
Bse118I RCCGGY 1 cut(s) 1362
Bse1I ACTGG 2 cut(s) 74, 818
Bse3DI GCAATG 2 cut(s) 975, 1090
BseAI TCCGGA 1 cut(s) 553
BseBI CCWGG 3 cut(s) 491, 542, 1314
BseDI CCNNGG 5 cut(s) 541, 874, 909, 1014, 1155
BseGI GGATG 2 cut(s) 706, 1243
BseLI CCNNNNNNNGG 3 cut(s) 125, 187, 1106
BseMI GCAATG 2 cut(s) 975, 1090
BseMII CTCAG 2 cut(s) 530, 1437
BseNI ACTGG 2 cut(s) 74, 818
BseRI GAGGAG 1 cut(s) 1301
BseXI GCAGC 1 cut(s) 388
BshFI GGCC 1 cut(s) 19
BshNI GGYRCC 2 cut(s) 1010, 1414
BsiHKAI GWGCWC 2 cut(s) 396, 963
BsiSI CCGG 4 cut(s) 554, 1038, 1363, 1418
BslI CCNNNNNNNGG 3 cut(s) 125, 187, 1106
BsmAI GTCTC 3 cut(s) 519, 555, 857
BsmBI CGTCTC 1 cut(s) 519
BsnI GGCC 1 cut(s) 19
Bsp1286I GDGCHC 2 cut(s) 396, 963
Bsp13I TCCGGA 1 cut(s) 553
Bsp143I GATC 3 cut(s) 648, 870, 919
Bsp19I CCATGG 2 cut(s) 909, 1155
BspACI CCGC 1 cut(s) 841
BspANI GGCC 1 cut(s) 19
BspCNI CTCAG 2 cut(s) 529, 1438
BspEI TCCGGA 1 cut(s) 553
BspHI TCATGA 1 cut(s) 322
BspLI GGNNCC 3 cut(s) 777, 1012, 1416
BspPI GGATC 2 cut(s) 656, 865
BspT107I GGYRCC 2 cut(s) 1010, 1414
BspTI CTTAAG 1 cut(s) 86
BsrBI CCGCTC 1 cut(s) 841
BsrDI GCAATG 2 cut(s) 975, 1090
BsrFI RCCGGY 1 cut(s) 1362
BsrI ACTGG 2 cut(s) 74, 818
BssAI RCCGGY 1 cut(s) 1362
BssECI CCNNGG 5 cut(s) 541, 874, 909, 1014, 1155
BssMI GATC 3 cut(s) 648, 870, 919
BssT1I CCWWGG 3 cut(s) 909, 1014, 1155
Bst2UI CCWGG 3 cut(s) 491, 542, 1314
Bst4CI ACNGT 4 cut(s) 128, 182, 566, 1105
Bst6I CTCTTC 3 cut(s) 314, 948, 1386
BstAFI CTTAAG 1 cut(s) 86
BstDEI CTNAG 3 cut(s) 516, 1188, 1446
BstDSI CCRYGG 2 cut(s) 909, 1155
BstF5I GGATG 2 cut(s) 706, 1243
BstKTI GATC 3 cut(s) 651, 873, 922
BstMAI GTCTC 3 cut(s) 519, 555, 857
BstMBI GATC 3 cut(s) 648, 870, 919
BstNI CCWGG 3 cut(s) 491, 542, 1314
BstSCI CCNGG 4 cut(s) 489, 540, 1037, 1312
BstSFI CTRYAG 2 cut(s) 901, 1030
BstV1I GCAGC 1 cut(s) 388
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 1 cut(s) 648
BstYI RGATCY 1 cut(s) 648
BsuRI GGCC 1 cut(s) 19
BtgI CCRYGG 2 cut(s) 909, 1155
BtgZI GCGATG 1 cut(s) 1155
BtsCI GGATG 2 cut(s) 706, 1243
BtsI GCAGTG 1 cut(s) 832
BtsIMutI CAGTG 3 cut(s) 81, 811, 832
CaiI CAGNNNCTG 1 cut(s) 1061
CciI TCATGA 1 cut(s) 322
Cfr10I RCCGGY 1 cut(s) 1362
Cfr13I GGNCC 1 cut(s) 1360
CseI GACGC 1 cut(s) 501
Csp6I GTAC 2 cut(s) 267, 1415
CviAII CATG 5 cut(s) 154, 323, 910, 1156, 1282
CviQI GTAC 2 cut(s) 267, 1415
DdeI CTNAG 3 cut(s) 516, 1188, 1446
DpnI GATC 3 cut(s) 650, 872, 921
DpnII GATC 3 cut(s) 648, 870, 919
DriI GACNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 1 cut(s) 17
Eam1104I CTCTTC 3 cut(s) 314, 948, 1386
Eam1105I GACNNNNNGTC 1 cut(s) 511
EarI CTCTTC 3 cut(s) 314, 948, 1386
Ecl136II GAGCTC 1 cut(s) 394
Eco130I CCWWGG 3 cut(s) 909, 1014, 1155
Eco24I GRGCYC 1 cut(s) 396
Eco32I GATATC 1 cut(s) 1172
Eco47I GGWCC 1 cut(s) 1360
Eco53kI GAGCTC 1 cut(s) 394
Eco57I CTGAAG 1 cut(s) 400
EcoICRI GAGCTC 1 cut(s) 394
EcoRI GAATTC 2 cut(s) 445, 690
EcoRII CCWGG 3 cut(s) 489, 540, 1312
EcoRV GATATC 1 cut(s) 1172
EcoT14I CCWWGG 3 cut(s) 909, 1014, 1155
EcoT22I ATGCAT 1 cut(s) 706
EcoT38I GRGCYC 1 cut(s) 396
ErhI CCWWGG 3 cut(s) 909, 1014, 1155
Esp3I CGTCTC 1 cut(s) 519
FaeI CATG 5 cut(s) 157, 326, 913, 1159, 1285
FalI AAGNNNNNCTT 2 cut(s) 924, 956
FatI CATG 5 cut(s) 153, 322, 909, 1155, 1281
FauI CCCGC 1 cut(s) 834
FauNDI CATATG 1 cut(s) 1332
Fnu4HI GCNGC 1 cut(s) 402
FokI GGATG 2 cut(s) 713, 1250
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 1 cut(s) 402
FspBI CTAG 2 cut(s) 596, 1232
GluI GCNGC 1 cut(s) 402
GsuI CTGGAG 2 cut(s) 259, 664
HaeIII GGCC 1 cut(s) 19
HapII CCGG 4 cut(s) 554, 1038, 1363, 1418
HgaI GACGC 1 cut(s) 501
Hin1II CATG 5 cut(s) 157, 326, 913, 1159, 1285
HincII GTYRAC 1 cut(s) 858
HindII GTYRAC 1 cut(s) 858
HindIII AAGCTT 4 cut(s) 89, 938, 1043, 1437
HinfI GANTC 8 cut(s) 70, 224, 242, 346, 386, 1273, 1421, 1448
HpaI GTTAAC 1 cut(s) 858
HpaII CCGG 4 cut(s) 554, 1038, 1363, 1418
HphI GGTGA 6 cut(s) 454, 614, 680, 731, 856, 962
Hpy166II GTNNAC 1 cut(s) 858
Hpy188I TCNGA 7 cut(s) 120, 391, 419, 465, 505, 780, 875
Hpy8I GTNNAC 1 cut(s) 858
HpyAV CCTTC 8 cut(s) 211, 279, 329, 472, 577, 926, 941, 1106
HpyCH4III ACNGT 4 cut(s) 128, 182, 566, 1105
HpyCH4IV ACGT 2 cut(s) 1119, 1510
HpyCH4V TGCA 5 cut(s) 254, 704, 881, 968, 1084
HpyF3I CTNAG 3 cut(s) 516, 1188, 1446
HpySE526I ACGT 2 cut(s) 1119, 1510
Hsp92II CATG 5 cut(s) 157, 326, 913, 1159, 1285
Kpn2I TCCGGA 1 cut(s) 553
KpnI GGTACC 1 cut(s) 1418
KspAI GTTAAC 1 cut(s) 858
Kzo9I GATC 3 cut(s) 648, 870, 919
LmnI GCTCC 2 cut(s) 781, 966
Lsp1109I GCAGC 1 cut(s) 388
LweI GCATC 7 cut(s) 263, 286, 691, 703, 721, 1071, 1501
MaeI CTAG 2 cut(s) 596, 1232
MaeII ACGT 2 cut(s) 1119, 1510
MaeIII GTNAC 6 cut(s) 76, 122, 128, 566, 788, 809
MalI GATC 3 cut(s) 650, 872, 921
MbiI CCGCTC 1 cut(s) 841
MboI GATC 3 cut(s) 648, 870, 919
MfeI CAATTG 1 cut(s) 331
MflI RGATCY 1 cut(s) 648
MhlI GDGCHC 2 cut(s) 396, 963
MlsI TGGCCA 1 cut(s) 19
MluNI TGGCCA 1 cut(s) 19
MlyI GAGTC 3 cut(s) 355, 1282, 1457
MmeI TCCRAC 5 cut(s) 168, 1087, 1124, 1147, 1368
Mox20I TGGCCA 1 cut(s) 19
Mph1103I ATGCAT 1 cut(s) 706
MroI TCCGGA 1 cut(s) 553
MscI TGGCCA 1 cut(s) 19
MseI TTAA 7 cut(s) 87, 381, 770, 857, 1020, 1047, 1513
Msp20I TGGCCA 1 cut(s) 19
MspCI CTTAAG 1 cut(s) 86
MspI CCGG 4 cut(s) 554, 1038, 1363, 1418
MspR9I CCNGG 4 cut(s) 491, 542, 1039, 1314
MunI CAATTG 1 cut(s) 331
MvaI CCWGG 3 cut(s) 491, 542, 1314
NciI CCSGG 1 cut(s) 1039
NcoI CCATGG 2 cut(s) 909, 1155
NdeI CATATG 1 cut(s) 1332
NdeII GATC 3 cut(s) 648, 870, 919
NlaIII CATG 5 cut(s) 157, 326, 913, 1159, 1285
NlaIV GGNNCC 3 cut(s) 777, 1012, 1416
NmuCI GTSAC 3 cut(s) 76, 128, 809
NsiI ATGCAT 1 cut(s) 706
PagI TCATGA 1 cut(s) 322
PfeI GAWTC 5 cut(s) 70, 224, 242, 386, 1421
PflMI CCANNNNNTGG 1 cut(s) 1106
PkrI GCNGC 1 cut(s) 403
PleI GAGTC 3 cut(s) 354, 1281, 1456
PpsI GAGTC 3 cut(s) 354, 1281, 1456
PsiI TTATAA 1 cut(s) 1127
Psp124BI GAGCTC 1 cut(s) 396
Psp1406I AACGTT 2 cut(s) 1119, 1510
Psp6I CCWGG 3 cut(s) 489, 540, 1312
PspGI CCWGG 3 cut(s) 489, 540, 1312
PspN4I GGNNCC 3 cut(s) 777, 1012, 1416
PspPI GGNCC 1 cut(s) 1360
PsrI GAACNNNNNNTAC 2 cut(s) 623, 655
PstNI CAGNNNCTG 1 cut(s) 1061
PsuI RGATCY 1 cut(s) 648
RsaI GTAC 2 cut(s) 268, 1416
RsaNI GTAC 2 cut(s) 267, 1415
SacI GAGCTC 1 cut(s) 396
SaqAI TTAA 7 cut(s) 87, 381, 770, 857, 1020, 1047, 1513
SatI GCNGC 1 cut(s) 402
Sau3AI GATC 3 cut(s) 648, 870, 919
Sau96I GGNCC 1 cut(s) 1360
SchI GAGTC 3 cut(s) 355, 1282, 1457
ScrFI CCNGG 4 cut(s) 491, 542, 1039, 1314
SduI GDGCHC 2 cut(s) 396, 963
SfaNI GCATC 7 cut(s) 263, 286, 691, 703, 721, 1071, 1501
SfcI CTRYAG 2 cut(s) 901, 1030
SinI GGWCC 1 cut(s) 1360
SmlI CTYRAG 5 cut(s) 86, 608, 757, 1265, 1487
SmoI CTYRAG 5 cut(s) 86, 608, 757, 1265, 1487
SsiI CCGC 1 cut(s) 841
SspMI CTAG 2 cut(s) 596, 1232
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 4 cut(s) 489, 540, 1037, 1312
StyI CCWWGG 3 cut(s) 909, 1014, 1155
TaaI ACNGT 4 cut(s) 128, 182, 566, 1105
TaiI ACGT 2 cut(s) 1122, 1513
TaqI TCGA 5 cut(s) 357, 396, 451, 1249, 1320
TfiI GAWTC 5 cut(s) 70, 224, 242, 386, 1421
Tru1I TTAA 7 cut(s) 87, 381, 770, 857, 1020, 1047, 1513
Tru9I TTAA 7 cut(s) 87, 381, 770, 857, 1020, 1047, 1513
TscAI CASTG 3 cut(s) 81, 818, 832
TseFI GTSAC 3 cut(s) 76, 128, 809
TseI GCWGC 1 cut(s) 401
Tsp45I GTSAC 3 cut(s) 76, 128, 809
TspDTI ATGAA 7 cut(s) 209, 230, 311, 491, 548, 596, 1386
TspRI CASTG 3 cut(s) 81, 818, 832
Van91I CCANNNNNTGG 1 cut(s) 1106
Vha464I CTTAAG 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 1360
XapI RAATTY 4 cut(s) 445, 466, 690, 741
XbaI TCTAGA 1 cut(s) 595
XspI CTAG 2 cut(s) 596, 1232
Zsp2I ATGCAT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.