AT1G22920

protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex (By similarity). The CSN complex is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of PSIAA6 by regulating the activity of the Ubl ligase SCF-TIR complex. Involved in CSN's deneddylation derubylation activity. Required for the deneddylation of all cullins. Essential for the structural integrity of the CSN holocomplex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011166
Physical Location & Seq
Reverse (-)
8109574 .. 8112061
2488 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G22920.1

Sequence Viewer

Length: 1074 bp
ATGGAAGGTTCCTCGTCAGCCATCGCGAGGAAGACATGGGAGCTAGAGAACAACATTCTCCCAGTGGAACCAACCGATTCAGCCTCCGACAGTATATTCCACTACGACGACGCTTCACAAGCCAAAATCCAGCAGGAGAAGCCATGGGCCTCCGATCCTAACTACTTCAAGCGCGTTCACATCTCAGCCCTTGCTCTTCTCAAGATGGTGGTTCACGCTCGCTCCGGTGGCACAATCGAGATCATGGGTCTTATGCAGGGTAAAACCGAGGGTGATACAATCATCGTTATGGATGCTTTTGCTTTGCCTGTTGAAGGTACTGAGACTAGGGTTAATGCTCAGTCTGATGCCTATGAGTATATGGTTGAATACTCTCAGACCAGCAAGCTGGCTGGGAGGTTGGAGAACGTTGTTGGATGGTATCACTCTCACCCTGGGTATGGATGTTGGCTCTCGGGTATTGATGTTTCGACACAGATGCTTAACCAACAGTATCAGGAGCCATTCTTAGCTGTTGTTATTGATCCAACAAGGACTGTTTCGGCTGGTAAGGTTGAGATTGGGGCATTCAGAACATATCCAGAGGGACATAAGATCTCGGATGATCATGTTTCTGAGTATCAGACTATCCCTCTTAACAAGATTGAGGACTTTGGTGTACATTGCAAACAGTACTACTCATTGGACATCACTTATTTCAAGTCATCTCTCGATAGTCACCTTCTGGATCTCCTTTGGAACAAGTACTGGGTGAACACTCTTTCTTCTTCCCCACTGTTGGGCAATGGAGACTATGTTGCCGGGCAAATATCAGACTTGGCTGAGAAGCTCGAGCAAGCGGAGAGTCAGCTCGCTAACTCCCGGTATGGAGGAATTGCGCCAGCCGGTCACCAAAGGAGGAAAGAGGATGAGCCTCAACTCGCGAAGATAACTCGGGATAGTGCAAAGATAACTGTCGAGCAGGTCCATGGACTAATGTCACAGGTTATCAAAGACATCTTGTTCAATTCCGCTCGTCAGTCCAAGAAGTCTGCTGACGACTCATCAGATCCAGAGCCCATGATTACATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000338 GO:0003002 GO:0003006 GO:0006109 GO:0006464 GO:0006508 GO:0006807 GO:0007275 GO:0007346 GO:0007389 GO:0008150 GO:0008152 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009889 GO:0009893 GO:0009894 GO:0009896 GO:0009908 GO:0009987 GO:0010033 GO:0010093 GO:0010099 GO:0010100 GO:0010387 GO:0010389 GO:0010564 GO:0010565 GO:0010604 GO:0010971 GO:0016043 GO:0019222 GO:0019538 GO:0022414 GO:0022607 GO:0030656 GO:0031323 GO:0031326 GO:0031347 GO:0032501 GO:0032502 GO:0034622 GO:0036211 GO:0042176 GO:0042221 GO:0043170 GO:0043255 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0045732 GO:0045787 GO:0045931 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051173 GO:0051193 GO:0051196 GO:0051239 GO:0051241 GO:0051246 GO:0051247 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0065003 GO:0065007 GO:0070646 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090068 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0099402 GO:1901564 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902749 GO:1902751 GO:1905392 GO:1905393 GO:2000026 GO:2000030 GO:2000082
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
JAB

Protein Analysis

357

Amino Acids

39.73

Weight (kDa)

5.11

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 55 - 167 3.4e-37 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
CSN5_C PF18323 255 - 336 6.6e-28 Cop9 signalosome subunit 5 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011166)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22920 AT1G22920 AT1G71230
fragaria_vesca FvH4_2g36890 FvH4_2g36890 FvH4_2g36890
malus_domestica MD08G1130400.v1.1 MD15G1108500.v1.1
prunus_persica Prupe.1G460800_v2.0.a1
pyrus_communis pycom08g10950
rosa_chinensis RchiOBHm_Chr6g0304891
rosa_laevigata RLG00000010941
rosa_multiflora Rmu_ssc0000278.1_g000004
rosa_roxburghii Rroxscaffold_7G00163370
rosa_rugosa Rorug06G0336100
rosa_samantha Rh6AG447300 Rh6BG454200 Rh6CG460800 Rh6DG447800
rosa_wichuraiana Rw6G038940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 952
AccBSI CCGCTC 1 cut(s) 1013
AccII CGCG 3 cut(s) 26, 174, 923
AciI CCGC 2 cut(s) 839, 1011
AclI AACGTT 1 cut(s) 408
AclWI GGATC 4 cut(s) 149, 518, 735, 1043
AfaI GTAC 4 cut(s) 319, 660, 674, 746
AfiI CCNNNNNNNGG 5 cut(s) 27, 314, 440, 778, 779
AgsI TTSAA 5 cut(s) 169, 314, 368, 700, 1006
AjnI CCWGG 1 cut(s) 433
AluBI AGCT 5 cut(s) 43, 388, 512, 829, 850
AluI AGCT 5 cut(s) 43, 388, 512, 829, 850
Alw26I GTCTC 2 cut(s) 317, 783
AlwI GGATC 4 cut(s) 149, 518, 735, 1043
Ama87I CYCGRG 3 cut(s) 454, 830, 933
AoxI GGCC 1 cut(s) 147
AspLEI GCGC 2 cut(s) 174, 880
AspS9I GGNCC 2 cut(s) 147, 964
AsuC2I CCSGG 2 cut(s) 802, 862
AsuHPI GGTGA 5 cut(s) 284, 422, 710, 763, 881
AvaI CYCGRG 3 cut(s) 454, 830, 933
AvaII GGWCC 1 cut(s) 964
BanII GRGCYC 1 cut(s) 1059
BbsI GAAGAC 1 cut(s) 38
BccI CCATC 3 cut(s) 29, 199, 411
BcgI CGANNNNNNTGC 2 cut(s) 460, 494
BciT130I CCWGG 1 cut(s) 435
BclI TGATCA 1 cut(s) 604
BcnI CCSGG 2 cut(s) 802, 862
BcoDI GTCTC 2 cut(s) 317, 783
BfaI CTAG 2 cut(s) 44, 327
BfuAI ACCTGC 1 cut(s) 952
BglII AGATCT 1 cut(s) 594
BmcAI AGTACT 2 cut(s) 674, 746
Bme1390I CCNGG 3 cut(s) 435, 802, 862
Bme18I GGWCC 1 cut(s) 964
BmeT110I CYCGRG 3 cut(s) 454, 830, 933
BmgT120I GGNCC 2 cut(s) 147, 964
BmiI GGNNCC 3 cut(s) 10, 69, 501
BmrFI CCNGG 3 cut(s) 435, 802, 862
BmrI ACTGGG 2 cut(s) 56, 757
BmsI GCATC 3 cut(s) 283, 337, 468
BmuI ACTGGG 2 cut(s) 56, 757
BoxI GACNNNNGTC 1 cut(s) 976
BpiI GAAGAC 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 185
BpuMI CCSGG 2 cut(s) 802, 862
BsaJI CCNNGG 5 cut(s) 143, 267, 433, 434, 967
BsaWI WCCGGW 1 cut(s) 224
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 780, 810
Bsc4I CCNNNNNNNGG 5 cut(s) 27, 314, 440, 778, 779
Bse118I RCCGGY 1 cut(s) 884
Bse1I ACTGG 2 cut(s) 62, 752
Bse3DI GCAATG 2 cut(s) 661, 790
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 5 cut(s) 143, 267, 433, 434, 967
BseGI GGATG 5 cut(s) 298, 422, 449, 607, 913
BseLI CCNNNNNNNGG 5 cut(s) 27, 314, 440, 778, 779
BseMI GCAATG 2 cut(s) 661, 790
BseMII CTCAG 6 cut(s) 198, 312, 353, 389, 606, 813
BseNI ACTGG 2 cut(s) 62, 752
BseYI CCCAGC 1 cut(s) 392
Bsh1236I CGCG 3 cut(s) 26, 174, 923
BshFI GGCC 1 cut(s) 149
BsiHKCI CYCGRG 3 cut(s) 454, 830, 933
BsiSI CCGG 4 cut(s) 225, 801, 862, 885
BslFI GGGAC 1 cut(s) 600
BslI CCNNNNNNNGG 5 cut(s) 27, 314, 440, 778, 779
BsmAI GTCTC 2 cut(s) 317, 783
BsmFI GGGAC 1 cut(s) 600
BsmI GAATGC 1 cut(s) 566
BsnI GGCC 1 cut(s) 149
BsoBI CYCGRG 3 cut(s) 454, 830, 933
Bsp1286I GDGCHC 1 cut(s) 1059
Bsp1407I TGTACA 1 cut(s) 658
Bsp143I GATC 7 cut(s) 154, 240, 523, 594, 604, 727, 1048
Bsp19I CCATGG 2 cut(s) 143, 967
Bsp68I TCGCGA 2 cut(s) 26, 923
BspACI CCGC 2 cut(s) 839, 1011
BspANI GGCC 1 cut(s) 149
BspCNI CTCAG 6 cut(s) 197, 313, 352, 388, 607, 814
BspFNI CGCG 3 cut(s) 26, 174, 923
BspLI GGNNCC 3 cut(s) 10, 69, 501
BspMI ACCTGC 1 cut(s) 952
BspPI GGATC 4 cut(s) 149, 518, 735, 1043
BspQI GCTCTTC 1 cut(s) 201
BsrBI CCGCTC 1 cut(s) 1013
BsrDI GCAATG 2 cut(s) 661, 790
BsrFI RCCGGY 1 cut(s) 884
BsrGI TGTACA 1 cut(s) 658
BsrI ACTGG 2 cut(s) 62, 752
BssAI RCCGGY 1 cut(s) 884
BssECI CCNNGG 5 cut(s) 143, 267, 433, 434, 967
BssMI GATC 7 cut(s) 154, 240, 523, 594, 604, 727, 1048
BssT1I CCWWGG 2 cut(s) 143, 967
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 6 cut(s) 92, 492, 538, 672, 777, 955
Bst6I CTCTTC 1 cut(s) 201
BstAUI TGTACA 1 cut(s) 658
BstC8I GCNNGC 6 cut(s) 220, 386, 390, 837, 852, 882
BstDEI CTNAG 7 cut(s) 184, 321, 339, 375, 508, 615, 822
BstDSI CCRYGG 2 cut(s) 143, 967
BstEII GGTNACC 1 cut(s) 887
BstENI CCTNNNNNAGG 1 cut(s) 312
BstF5I GGATG 5 cut(s) 298, 422, 449, 607, 913
BstFNI CGCG 3 cut(s) 26, 174, 923
BstHHI GCGC 2 cut(s) 174, 880
BstKTI GATC 7 cut(s) 157, 243, 526, 597, 607, 730, 1051
BstMAI GTCTC 2 cut(s) 317, 783
BstMBI GATC 7 cut(s) 154, 240, 523, 594, 604, 727, 1048
BstMWI GCNNNNNNNGC 3 cut(s) 119, 139, 228
BstNI CCWGG 1 cut(s) 435
BstPAI GACNNNNGTC 1 cut(s) 976
BstPI GGTNACC 1 cut(s) 887
BstSCI CCNGG 3 cut(s) 433, 800, 860
BstUI CGCG 3 cut(s) 26, 174, 923
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 3 cut(s) 594, 727, 1048
BstXI CCANNNNNNTGG 1 cut(s) 388
BstYI RGATCY 3 cut(s) 594, 727, 1048
BsuRI GGCC 1 cut(s) 149
BtgI CCRYGG 2 cut(s) 143, 967
BtgZI GCGATG 1 cut(s) 7
BtsCI GGATG 5 cut(s) 298, 422, 449, 607, 913
BtsIMutI CAGTG 2 cut(s) 69, 773
BtuMI TCGCGA 2 cut(s) 26, 923
BveI ACCTGC 1 cut(s) 952
Cac8I GCNNGC 6 cut(s) 220, 386, 390, 837, 852, 882
CfoI GCGC 2 cut(s) 174, 880
Cfr10I RCCGGY 1 cut(s) 884
Cfr13I GGNCC 2 cut(s) 147, 964
CseI GACGC 1 cut(s) 119
Csp6I GTAC 4 cut(s) 318, 659, 673, 745
CviAII CATG 6 cut(s) 36, 144, 244, 608, 968, 1060
CviQI GTAC 4 cut(s) 318, 659, 673, 745
DdeI CTNAG 7 cut(s) 184, 321, 339, 375, 508, 615, 822
DpnI GATC 7 cut(s) 156, 242, 525, 596, 606, 729, 1050
DpnII GATC 7 cut(s) 154, 240, 523, 594, 604, 727, 1048
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco130I CCWWGG 2 cut(s) 143, 967
Eco24I GRGCYC 1 cut(s) 1059
Eco47I GGWCC 1 cut(s) 964
Eco88I CYCGRG 3 cut(s) 454, 830, 933
Eco91I GGTNACC 1 cut(s) 887
EcoNI CCTNNNNNAGG 1 cut(s) 312
EcoO65I GGTNACC 1 cut(s) 887
EcoRII CCWGG 1 cut(s) 433
EcoT14I CCWWGG 2 cut(s) 143, 967
EcoT38I GRGCYC 1 cut(s) 1059
ErhI CCWWGG 2 cut(s) 143, 967
FaeI CATG 6 cut(s) 39, 147, 247, 611, 971, 1063
FaqI GGGAC 1 cut(s) 600
FatI CATG 6 cut(s) 35, 143, 243, 607, 967, 1059
FbaI TGATCA 1 cut(s) 604
FokI GGATG 5 cut(s) 305, 429, 456, 614, 920
FriOI GRGCYC 1 cut(s) 1059
FspBI CTAG 2 cut(s) 44, 327
GlaI GCGC 2 cut(s) 173, 879
GsaI CCCAGC 1 cut(s) 396
HaeIII GGCC 1 cut(s) 149
HapII CCGG 4 cut(s) 225, 801, 862, 885
HgaI GACGC 1 cut(s) 119
HhaI GCGC 2 cut(s) 174, 880
Hin1II CATG 6 cut(s) 39, 147, 247, 611, 971, 1063
Hin6I GCGC 2 cut(s) 172, 878
HinP1I GCGC 2 cut(s) 172, 878
HinfI GANTC 3 cut(s) 77, 844, 1040
HpaII CCGG 4 cut(s) 225, 801, 862, 885
HphI GGTGA 5 cut(s) 284, 422, 710, 763, 881
Hpy166II GTNNAC 4 cut(s) 178, 214, 659, 754
Hpy8I GTNNAC 4 cut(s) 178, 214, 659, 754
Hpy99I CGWCG 2 cut(s) 110, 113
HpyAV CCTTC 2 cut(s) 308, 731
HpyCH4III ACNGT 6 cut(s) 92, 492, 538, 672, 777, 955
HpyCH4IV ACGT 1 cut(s) 408
HpyCH4V TGCA 3 cut(s) 256, 666, 944
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 139, 228
HpyF3I CTNAG 7 cut(s) 184, 321, 339, 375, 508, 615, 822
HpySE526I ACGT 1 cut(s) 408
Hsp92II CATG 6 cut(s) 39, 147, 247, 611, 971, 1063
HspAI GCGC 2 cut(s) 172, 878
Ksp22I TGATCA 1 cut(s) 604
Kzo9I GATC 7 cut(s) 154, 240, 523, 594, 604, 727, 1048
LguI GCTCTTC 1 cut(s) 201
LmnI GCTCC 3 cut(s) 40, 227, 499
LweI GCATC 3 cut(s) 283, 337, 468
MaeI CTAG 2 cut(s) 44, 327
MaeII ACGT 1 cut(s) 408
MaeIII GTNAC 3 cut(s) 716, 887, 978
MalI GATC 7 cut(s) 156, 242, 525, 596, 606, 729, 1050
MbiI CCGCTC 1 cut(s) 1013
MboI GATC 7 cut(s) 154, 240, 523, 594, 604, 727, 1048
MboII GAAGA 5 cut(s) 43, 188, 756, 759, 937
MflI RGATCY 3 cut(s) 594, 727, 1048
MhlI GDGCHC 1 cut(s) 1059
MluCI AATT 2 cut(s) 873, 1006
MlyI GAGTC 2 cut(s) 853, 1034
MmeI TCCRAC 4 cut(s) 111, 381, 394, 551
MseI TTAA 3 cut(s) 333, 483, 636
MslI CAYNNNNRTG 1 cut(s) 287
MspI CCGG 4 cut(s) 225, 801, 862, 885
MspR9I CCNGG 3 cut(s) 435, 802, 862
Mva1269I GAATGC 1 cut(s) 566
MvaI CCWGG 1 cut(s) 435
MvnI CGCG 3 cut(s) 26, 174, 923
MwoI GCNNNNNNNGC 3 cut(s) 119, 139, 228
NciI CCSGG 2 cut(s) 802, 862
NcoI CCATGG 2 cut(s) 143, 967
NdeII GATC 7 cut(s) 154, 240, 523, 594, 604, 727, 1048
NlaIII CATG 6 cut(s) 39, 147, 247, 611, 971, 1063
NlaIV GGNNCC 3 cut(s) 10, 69, 501
NmuCI GTSAC 3 cut(s) 716, 887, 978
NruI TCGCGA 2 cut(s) 26, 923
PaeR7I CTCGAG 1 cut(s) 830
PasI CCCWGGG 1 cut(s) 434
PciSI GCTCTTC 1 cut(s) 201
PctI GAATGC 1 cut(s) 566
PfeI GAWTC 1 cut(s) 77
PleI GAGTC 2 cut(s) 852, 1034
PpsI GAGTC 2 cut(s) 852, 1034
PshAI GACNNNNGTC 1 cut(s) 976
Psp1406I AACGTT 1 cut(s) 408
Psp6I CCWGG 1 cut(s) 433
PspEI GGTNACC 1 cut(s) 887
PspFI CCCAGC 1 cut(s) 392
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 3 cut(s) 10, 69, 501
PspPI GGNCC 2 cut(s) 147, 964
PspXI VCTCGAGB 1 cut(s) 830
PsuI RGATCY 3 cut(s) 594, 727, 1048
RruI TCGCGA 2 cut(s) 26, 923
RsaI GTAC 4 cut(s) 319, 660, 674, 746
RsaNI GTAC 4 cut(s) 318, 659, 673, 745
RseI CAYNNNNRTG 1 cut(s) 287
SapI GCTCTTC 1 cut(s) 201
SaqAI TTAA 3 cut(s) 333, 483, 636
Sau3AI GATC 7 cut(s) 154, 240, 523, 594, 604, 727, 1048
Sau96I GGNCC 2 cut(s) 147, 964
ScaI AGTACT 2 cut(s) 674, 746
SchI GAGTC 2 cut(s) 853, 1034
ScrFI CCNGG 3 cut(s) 435, 802, 862
SduI GDGCHC 1 cut(s) 1059
SfaNI GCATC 3 cut(s) 283, 337, 468
Sfr274I CTCGAG 1 cut(s) 830
SinI GGWCC 1 cut(s) 964
SlaI CTCGAG 1 cut(s) 830
SmiMI CAYNNNNRTG 1 cut(s) 287
SmlI CTYRAG 2 cut(s) 200, 830
SmoI CTYRAG 2 cut(s) 200, 830
Sse9I AATT 2 cut(s) 873, 1006
SsiI CCGC 2 cut(s) 839, 1011
SspMI CTAG 2 cut(s) 44, 327
StyD4I CCNGG 3 cut(s) 433, 800, 860
StyI CCWWGG 2 cut(s) 143, 967
TaaI ACNGT 6 cut(s) 92, 492, 538, 672, 777, 955
TaiI ACGT 1 cut(s) 411
TaqI TCGA 5 cut(s) 237, 470, 711, 831, 957
TasI AATT 2 cut(s) 873, 1006
TatI WGTACW 3 cut(s) 658, 672, 744
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 3 cut(s) 333, 483, 636
Tru9I TTAA 3 cut(s) 333, 483, 636
TscAI CASTG 2 cut(s) 69, 780
TseFI GTSAC 3 cut(s) 716, 887, 978
Tsp45I GTSAC 3 cut(s) 716, 887, 978
TspRI CASTG 2 cut(s) 69, 780
VpaK11BI GGWCC 1 cut(s) 964
XagI CCTNNNNNAGG 1 cut(s) 312
XhoI CTCGAG 1 cut(s) 830
XspI CTAG 2 cut(s) 44, 327
ZrmI AGTACT 2 cut(s) 674, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.