AT1G71230

protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex (By similarity). The CSN complex is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of PSIAA6 by regulating the activity of the Ubl ligase SCF-TIR complex. Involved in CSN's deneddylation derubylation activity. Required for the deneddylation of all cullins. Essential for the structural integrity of the CSN holocomplex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011166
Physical Location & Seq
Forward (+)
26852406 .. 26854419
2014 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G71230.2

Sequence Viewer

Length: 1077 bp
ATGGAGGGTTCGTCGTCGACGATAGCAAGGAAGACATGGGAACTAGAGAACAGCATTCTAACAGTAGACTCACCTGATTCAACCTCCGACAACATCTTCTACTACGACGATACTTCACAGACTAGGTTCCAGCAAGAGAAACCGTGGGAGAATGATCCTCACTACTTTAAACGAGTCAAGATCTCAGCGCTCGCTCTTCTTAAGATGGTGGTTCACGCTCGCTCTGGTGGTACAATTGAAATAATGGGTCTTATGCAAGGTAAGACCGATGGTGATACTATCATTGTTATGGATGCTTTTGCTTTACCAGTGGAAGGTACTGAGACAAGGGTTAATGCTCAGGATGATGCTTATGAGTACATGGTTGAGTATTCACAGACCAACAAGCTCGCGGGGCGGCTGGAGAATGTTGTTGGATGGTATCACTCTCACCCTGGATATGGATGCTGGCTCTCCGGTATTGATGTTTCTACGCAGAGGCTTAACCAACAGCATCAGGAGCCATTTTTAGCTGTTGTTATTGATCCCACAAGGACTGTTTCAGCTGGTAAGGTTGAGATTGGTGCTTTCAGAACATACTCTAAAGGATATAAGCCTCCAGATGAACCTGTTTCTGAGTATCAAACTATTCCTTTAAATAAGATTGAGGACTTTGGTGTTCACTGCAAACAGTACTATTCATTAGATGTCACTTATTTCAAGTCATCTCTTGATTCTCACCTTCTGGATCTACTATGGAACAAGTACTGGGTGAACACTCTTTCTTCTTCTCCACTGCTGGGTAATGGAGACTATGTTGCTGGACAAATATCAGACTTAGCTGAGAAGCTTGAGCAAGCCGAGAGTCATCTGGTTCAGTCTCGCTTTGGAGGAGTTGTGCCATCATCCCTTCATAAGAAAAAAGAGGATGAGTCTCAACTAACTAAGATAACTCGGGATAGCGCAAAGATAACTGTGGAACAGGTCCATGGACTAATGTCGCAGGTCATAAAAGATGAATTATTCAACTCAATGCGTCAGTCCAACAACAAATCTCCCACTGACTCGTCGGATCCAGACCCTATGATTACATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000338 GO:0003002 GO:0003006 GO:0006109 GO:0006464 GO:0006508 GO:0006807 GO:0007275 GO:0007346 GO:0007389 GO:0008150 GO:0008152 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009889 GO:0009893 GO:0009894 GO:0009896 GO:0009908 GO:0009987 GO:0010033 GO:0010093 GO:0010099 GO:0010100 GO:0010387 GO:0010389 GO:0010564 GO:0010565 GO:0010604 GO:0010971 GO:0016043 GO:0019222 GO:0019538 GO:0022414 GO:0022607 GO:0030656 GO:0031323 GO:0031326 GO:0031347 GO:0032501 GO:0032502 GO:0034622 GO:0036211 GO:0042176 GO:0042221 GO:0043170 GO:0043255 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0045732 GO:0045787 GO:0045931 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051173 GO:0051193 GO:0051196 GO:0051239 GO:0051241 GO:0051246 GO:0051247 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0065003 GO:0065007 GO:0070646 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090068 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0099402 GO:1901564 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902749 GO:1902751 GO:1905392 GO:1905393 GO:2000026 GO:2000030 GO:2000082
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
JAB

Protein Analysis

358

Amino Acids

40.32

Weight (kDa)

5.11

Isoelectric Point (pI)

45.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011166)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22920 AT1G22920 AT1G71230
fragaria_vesca FvH4_2g36890 FvH4_2g36890 FvH4_2g36890
malus_domestica MD08G1130400.v1.1 MD15G1108500.v1.1
prunus_persica Prupe.1G460800_v2.0.a1
pyrus_communis pycom08g10950
rosa_chinensis RchiOBHm_Chr6g0304891
rosa_laevigata RLG00000010941
rosa_multiflora Rmu_ssc0000278.1_g000004
rosa_roxburghii Rroxscaffold_7G00163370
rosa_rugosa Rorug06G0336100
rosa_samantha Rh6AG447300 Rh6BG454200 Rh6CG460800 Rh6DG447800
rosa_wichuraiana Rw6G038940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 973
AccI GTMKAC 2 cut(s) 17, 66
AccII CGCG 1 cut(s) 392
AciI CCGC 2 cut(s) 392, 397
AclWI GGATC 5 cut(s) 149, 518, 735, 1046, 1059
AfaI GTAC 5 cut(s) 232, 319, 359, 674, 746
AfeI AGCGCT 1 cut(s) 189
AfiI CCNNNNNNNGG 3 cut(s) 314, 440, 779
AflII CTTAAG 1 cut(s) 200
AgsI TTSAA 4 cut(s) 81, 239, 700, 1006
AjnI CCWGG 1 cut(s) 433
AluBI AGCT 5 cut(s) 388, 512, 545, 821, 829
AluI AGCT 5 cut(s) 388, 512, 545, 821, 829
Alw26I GTCTC 4 cut(s) 317, 783, 864, 918
AlwI GGATC 5 cut(s) 149, 518, 735, 1046, 1059
Ama87I CYCGRG 1 cut(s) 933
Aor51HI AGCGCT 1 cut(s) 189
AspLEI GCGC 2 cut(s) 190, 944
AspS9I GGNCC 1 cut(s) 964
AsuHPI GGTGA 5 cut(s) 63, 284, 422, 710, 763
AvaI CYCGRG 1 cut(s) 933
AvaII GGWCC 1 cut(s) 964
BamHI GGATCC 1 cut(s) 1051
BbsI GAAGAC 1 cut(s) 38
BccI CCATC 4 cut(s) 199, 263, 411, 889
BciT130I CCWGG 1 cut(s) 435
BcoDI GTCTC 4 cut(s) 317, 783, 864, 918
BfaI CTAG 2 cut(s) 44, 123
BfoI RGCGCY 1 cut(s) 191
BfrI CTTAAG 1 cut(s) 200
BfuAI ACCTGC 1 cut(s) 973
BglII AGATCT 1 cut(s) 180
BisI GCNGC 1 cut(s) 398
BlsI GCNGC 1 cut(s) 399
BmcAI AGTACT 2 cut(s) 674, 746
Bme1390I CCNGG 1 cut(s) 435
Bme18I GGWCC 1 cut(s) 964
BmeT110I CYCGRG 1 cut(s) 933
BmgT120I GGNCC 1 cut(s) 964
BmiI GGNNCC 3 cut(s) 128, 501, 1053
BmrFI CCNGG 1 cut(s) 435
BmrI ACTGGG 1 cut(s) 757
BmsI GCATC 4 cut(s) 283, 337, 434, 502
BmuI ACTGGG 1 cut(s) 757
BoxI GACNNNNGTC 1 cut(s) 976
BpiI GAAGAC 1 cut(s) 38
BpmI CTGGAG 2 cut(s) 422, 582
Bpu10I CCTNAGC 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 851
BsaJI CCNNGG 3 cut(s) 143, 433, 967
BsaWI WCCGGW 1 cut(s) 455
BsaXI ACNNNNNCTCC 5 cut(s) 26, 780, 810, 861, 891
Bsc4I CCNNNNNNNGG 3 cut(s) 314, 440, 779
Bse1I ACTGG 2 cut(s) 308, 752
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 3 cut(s) 143, 433, 967
BseGI GGATG 6 cut(s) 298, 349, 422, 449, 884, 913
BseLI CCNNNNNNNGG 3 cut(s) 314, 440, 779
BseMII CTCAG 5 cut(s) 198, 312, 353, 606, 813
BseNI ACTGG 2 cut(s) 308, 752
BseRI GAGGAG 1 cut(s) 885
BseYI CCCAGC 1 cut(s) 778
Bsh1236I CGCG 1 cut(s) 392
BsiHKCI CYCGRG 1 cut(s) 933
BsiSI CCGG 1 cut(s) 456
BslI CCNNNNNNNGG 3 cut(s) 314, 440, 779
BsmAI GTCTC 4 cut(s) 317, 783, 864, 918
BsmI GAATGC 1 cut(s) 54
BsoBI CYCGRG 1 cut(s) 933
Bsp143I GATC 5 cut(s) 154, 180, 523, 727, 1051
Bsp19I CCATGG 1 cut(s) 967
BspACI CCGC 2 cut(s) 392, 397
BspCNI CTCAG 5 cut(s) 197, 313, 352, 607, 814
BspFNI CGCG 1 cut(s) 392
BspLI GGNNCC 3 cut(s) 128, 501, 1053
BspMI ACCTGC 1 cut(s) 973
BspPI GGATC 5 cut(s) 149, 518, 735, 1046, 1059
BspQI GCTCTTC 1 cut(s) 201
BspTI CTTAAG 1 cut(s) 200
BsrI ACTGG 2 cut(s) 308, 752
BssECI CCNNGG 3 cut(s) 143, 433, 967
BssMI GATC 5 cut(s) 154, 180, 523, 727, 1051
BssT1I CCWWGG 1 cut(s) 967
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 5 cut(s) 64, 144, 538, 672, 955
Bst6I CTCTTC 1 cut(s) 201
BstAFI CTTAAG 1 cut(s) 200
BstC8I GCNNGC 5 cut(s) 192, 220, 390, 449, 837
BstDEI CTNAG 7 cut(s) 184, 321, 339, 615, 817, 822, 924
BstDSI CCRYGG 2 cut(s) 143, 967
BstF5I GGATG 6 cut(s) 298, 349, 422, 449, 884, 913
BstFNI CGCG 1 cut(s) 392
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 2 cut(s) 190, 944
BstKTI GATC 5 cut(s) 157, 183, 526, 730, 1054
BstMAI GTCTC 4 cut(s) 317, 783, 864, 918
BstMBI GATC 5 cut(s) 154, 180, 523, 727, 1051
BstMWI GCNNNNNNNGC 2 cut(s) 394, 499
BstNI CCWGG 1 cut(s) 435
BstPAI GACNNNNGTC 1 cut(s) 976
BstSCI CCNGG 1 cut(s) 433
BstUI CGCG 1 cut(s) 392
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 3 cut(s) 180, 727, 1051
BstYI RGATCY 3 cut(s) 180, 727, 1051
BtgI CCRYGG 2 cut(s) 143, 967
BtsCI GGATG 6 cut(s) 298, 349, 422, 449, 884, 913
BtsI GCAGTG 2 cut(s) 661, 773
BtsIMutI CAGTG 4 cut(s) 315, 661, 773, 1038
BveI ACCTGC 1 cut(s) 973
Cac8I GCNNGC 5 cut(s) 192, 220, 390, 449, 837
CfoI GCGC 2 cut(s) 190, 944
Cfr13I GGNCC 1 cut(s) 964
CseI GACGC 1 cut(s) 1004
Csp6I GTAC 5 cut(s) 231, 318, 358, 673, 745
CviAII CATG 3 cut(s) 36, 361, 968
CviQI GTAC 5 cut(s) 231, 318, 358, 673, 745
DdeI CTNAG 7 cut(s) 184, 321, 339, 615, 817, 822, 924
DpnI GATC 5 cut(s) 156, 182, 525, 729, 1053
DpnII GATC 5 cut(s) 154, 180, 523, 727, 1051
DraI TTTAAA 2 cut(s) 169, 636
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco130I CCWWGG 1 cut(s) 967
Eco47I GGWCC 1 cut(s) 964
Eco47III AGCGCT 1 cut(s) 189
Eco88I CYCGRG 1 cut(s) 933
EcoRII CCWGG 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 967
ErhI CCWWGG 1 cut(s) 967
FaeI CATG 3 cut(s) 39, 364, 971
FatI CATG 3 cut(s) 35, 360, 967
FauI CCCGC 1 cut(s) 385
FblI GTMKAC 2 cut(s) 17, 66
Fnu4HI GCNGC 1 cut(s) 398
FokI GGATG 6 cut(s) 305, 356, 429, 456, 871, 920
Fsp4HI GCNGC 1 cut(s) 398
FspBI CTAG 2 cut(s) 44, 123
GlaI GCGC 2 cut(s) 189, 943
GluI GCNGC 1 cut(s) 398
GsaI CCCAGC 1 cut(s) 782
GsuI CTGGAG 2 cut(s) 422, 582
HaeII RGCGCY 1 cut(s) 191
HapII CCGG 1 cut(s) 456
HgaI GACGC 1 cut(s) 1004
HhaI GCGC 2 cut(s) 190, 944
Hin1II CATG 3 cut(s) 39, 364, 971
Hin6I GCGC 2 cut(s) 188, 942
HinP1I GCGC 2 cut(s) 188, 942
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HindIII AAGCTT 1 cut(s) 827
HinfI GANTC 7 cut(s) 68, 77, 174, 713, 844, 911, 1043
HpaII CCGG 1 cut(s) 456
HphI GGTGA 5 cut(s) 63, 284, 422, 710, 763
Hpy166II GTNNAC 5 cut(s) 18, 67, 214, 661, 754
Hpy188I TCNGA 5 cut(s) 88, 572, 616, 814, 1051
Hpy188III TCNNGA 8 cut(s) 178, 341, 497, 599, 710, 725, 935, 1055
Hpy8I GTNNAC 5 cut(s) 18, 67, 214, 661, 754
Hpy99I CGWCG 5 cut(s) 16, 19, 22, 110, 1051
HpyAV CCTTC 3 cut(s) 308, 731, 899
HpyCH4III ACNGT 5 cut(s) 64, 144, 538, 672, 955
HpyCH4V TGCA 2 cut(s) 256, 666
HpyF10VI GCNNNNNNNGC 2 cut(s) 394, 499
HpyF3I CTNAG 7 cut(s) 184, 321, 339, 615, 817, 822, 924
Hsp92II CATG 3 cut(s) 39, 364, 971
HspAI GCGC 2 cut(s) 188, 942
Kzo9I GATC 5 cut(s) 154, 180, 523, 727, 1051
LguI GCTCTTC 1 cut(s) 201
LmnI GCTCC 1 cut(s) 499
LweI GCATC 4 cut(s) 283, 337, 434, 502
MaeI CTAG 2 cut(s) 44, 123
MaeIII GTNAC 1 cut(s) 688
MalI GATC 5 cut(s) 156, 182, 525, 729, 1053
MboI GATC 5 cut(s) 154, 180, 523, 727, 1051
MboII GAAGA 5 cut(s) 43, 88, 188, 756, 759
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 3 cut(s) 180, 727, 1051
MluCI AATT 2 cut(s) 234, 998
MlyI GAGTC 5 cut(s) 62, 183, 853, 920, 1037
MmeI TCCRAC 4 cut(s) 111, 394, 1029, 1047
MnlI CCTC 7 cut(s) 94, 168, 471, 606, 640, 863, 898
MseI TTAA 5 cut(s) 168, 201, 333, 483, 635
MslI CAYNNNNRTG 1 cut(s) 287
MspA1I CMGCKG 1 cut(s) 545
MspCI CTTAAG 1 cut(s) 200
MspI CCGG 1 cut(s) 456
MspR9I CCNGG 1 cut(s) 435
MunI CAATTG 1 cut(s) 234
Mva1269I GAATGC 1 cut(s) 54
MvaI CCWGG 1 cut(s) 435
MvnI CGCG 1 cut(s) 392
MwoI GCNNNNNNNGC 2 cut(s) 394, 499
NcoI CCATGG 1 cut(s) 967
NdeII GATC 5 cut(s) 154, 180, 523, 727, 1051
NlaIII CATG 3 cut(s) 39, 364, 971
NlaIV GGNNCC 3 cut(s) 128, 501, 1053
NmeAIII GCCGAG 1 cut(s) 865
NmuCI GTSAC 1 cut(s) 688
PciSI GCTCTTC 1 cut(s) 201
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PctI GAATGC 1 cut(s) 54
PfeI GAWTC 2 cut(s) 77, 713
PkrI GCNGC 1 cut(s) 399
PleI GAGTC 5 cut(s) 62, 182, 852, 919, 1037
PpsI GAGTC 5 cut(s) 62, 182, 852, 919, 1037
PshAI GACNNNNGTC 1 cut(s) 976
Psp6I CCWGG 1 cut(s) 433
PspFI CCCAGC 1 cut(s) 778
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 3 cut(s) 128, 501, 1053
PspPI GGNCC 1 cut(s) 964
PsuI RGATCY 3 cut(s) 180, 727, 1051
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 5 cut(s) 232, 319, 359, 674, 746
RsaNI GTAC 5 cut(s) 231, 318, 358, 673, 745
RseI CAYNNNNRTG 1 cut(s) 287
SalI GTCGAC 1 cut(s) 16
SapI GCTCTTC 1 cut(s) 201
SaqAI TTAA 5 cut(s) 168, 201, 333, 483, 635
SatI GCNGC 1 cut(s) 398
Sau3AI GATC 5 cut(s) 154, 180, 523, 727, 1051
Sau96I GGNCC 1 cut(s) 964
ScaI AGTACT 2 cut(s) 674, 746
SchI GAGTC 5 cut(s) 62, 183, 853, 920, 1037
ScrFI CCNGG 1 cut(s) 435
SfaNI GCATC 4 cut(s) 283, 337, 434, 502
SgrDI CGTCGACG 1 cut(s) 16
SinI GGWCC 1 cut(s) 964
SmiMI CAYNNNNRTG 1 cut(s) 287
SmlI CTYRAG 2 cut(s) 200, 830
SmoI CTYRAG 2 cut(s) 200, 830
Sse9I AATT 2 cut(s) 234, 998
SsiI CCGC 2 cut(s) 392, 397
SspMI CTAG 2 cut(s) 44, 123
StyD4I CCNGG 1 cut(s) 433
StyI CCWWGG 1 cut(s) 967
TaaI ACNGT 5 cut(s) 64, 144, 538, 672, 955
TaqI TCGA 1 cut(s) 17
TaqII GACCGA 1 cut(s) 281
TasI AATT 2 cut(s) 234, 998
TatI WGTACW 3 cut(s) 357, 672, 744
TauI GCSGC 1 cut(s) 400
TfiI GAWTC 2 cut(s) 77, 713
Tru1I TTAA 5 cut(s) 168, 201, 333, 483, 635
Tru9I TTAA 5 cut(s) 168, 201, 333, 483, 635
TscAI CASTG 4 cut(s) 315, 668, 780, 1045
TseFI GTSAC 1 cut(s) 688
Tsp45I GTSAC 1 cut(s) 688
TspDTI ATGAA 4 cut(s) 618, 669, 881, 1011
TspRI CASTG 4 cut(s) 315, 668, 780, 1045
Vha464I CTTAAG 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 964
XmiI GTMKAC 2 cut(s) 17, 66
XspI CTAG 2 cut(s) 44, 123
ZrmI AGTACT 2 cut(s) 674, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.