Rh6CG460800

JAB/MPN domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0011166
Physical Location & Seq
Forward (+)
63478337 .. 63482044
3708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG460800.1

Sequence Viewer

Length: 1014 bp
ATGGATGCCGAAATAGCCCAGAAAACCTGGGAGCTCGAGAACAACATAGTCCCGATGGACACGCCTCCGTCCGGCGGCGCGGCGAGGACGAAGACGGACCCGTCGGCCGACGCCATCTTCTACTACGACGAGGCGGCGCAGACCAAGTTCCAGCAGGAGAAGCCGTGGGTTAACGACCCCCACTTCTTCAAGCGCGTCAAGATCTCCGCACTCGCGCTCCTGAAGATGGTCGTCCACGCGCGCTCCGGCGGCACCATCGAGGTCATGGGCCTCATGCAGGGCAAGACCGACGGCGACGCCATCATCGTCATGGACGCCTTTGCTTTGCCGGTTGAGGGCACCGAGACTAGGGTCAATGCCCAGGCCGATGCCTATGAGTATATGGTCGATTATTCCCAGACTAACAAACAGGCGGGGCGACTGGAGAATGTTGTTGGATGGTACCATTCTCACCCTGGTTATGGATGCTGGCTTTCGGGTATTGATGTTTCAACTCAGATGTTAAACCAGCAATTTCAGGAGCCCTTCTTGGCTGTTGTTATCGACCCAACTCGGACAGTGTCTGCTGGAAAAGTTGACATTGGTGCATTCAGGACATACCCAGAGGGATATAAGCCTCCAGATGATCCCATCTCCGAGTATCAGACTATTCCTCTTAACAAAATTGAGGACTTTGGTGTGCACTGTAAACAGTATTACTCTCTGGATATCACTTATTTCAAGTCTTGTCTTGACTCCCACCTCTTGGATCTGCTCTGGAACAAGTATTGGGTGAATACTCTCTCTTCCTCACCTTTGTTGGGTAATGGAGACTATGTTGCAGGACAAATTTCGGATTTAGCTGAAAAGCTGGAGCAAGCAGAGAATCATTTGTCTCATTCCCGCTTTGGACCATTAGTAACACCCACACAACGAAAGAAGGATGAAGAATCACAACTTGCTAAAATTACTAGGGATAGTGCAAAGATAACTGTGGAGCAGGTGCATGGTCTGATGTCACAGGTGAGCGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000338 GO:0003002 GO:0003006 GO:0006109 GO:0006464 GO:0006508 GO:0006807 GO:0007275 GO:0007346 GO:0007389 GO:0008150 GO:0008152 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009889 GO:0009893 GO:0009894 GO:0009896 GO:0009908 GO:0009987 GO:0010033 GO:0010093 GO:0010099 GO:0010100 GO:0010387 GO:0010389 GO:0010564 GO:0010565 GO:0010604 GO:0010971 GO:0016043 GO:0019222 GO:0019538 GO:0022414 GO:0022607 GO:0030656 GO:0031323 GO:0031326 GO:0031347 GO:0032501 GO:0032502 GO:0034622 GO:0036211 GO:0042176 GO:0042221 GO:0043170 GO:0043255 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0045732 GO:0045787 GO:0045931 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051173 GO:0051193 GO:0051196 GO:0051239 GO:0051241 GO:0051246 GO:0051247 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0065003 GO:0065007 GO:0070646 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090068 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0099402 GO:1901564 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902749 GO:1902751 GO:1905392 GO:1905393 GO:2000026 GO:2000030 GO:2000082
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
JAB

Protein Analysis

337

Amino Acids

37.65

Weight (kDa)

5.07

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 63 - 174 1.2e-36 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
CSN5_C PF18323 262 - 335 2.2e-24 Cop9 signalosome subunit 5 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011166)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22920 AT1G22920 AT1G71230
fragaria_vesca FvH4_2g36890 FvH4_2g36890 FvH4_2g36890
malus_domestica MD08G1130400.v1.1 MD15G1108500.v1.1
prunus_persica Prupe.1G460800_v2.0.a1
pyrus_communis pycom08g10950
rosa_chinensis RchiOBHm_Chr6g0304891
rosa_laevigata RLG00000010941
rosa_multiflora Rmu_ssc0000278.1_g000004
rosa_roxburghii Rroxscaffold_7G00163370
rosa_rugosa Rorug06G0336100
rosa_samantha Rh6AG447300 Rh6BG454200 Rh6CG460800 Rh6DG447800
rosa_wichuraiana Rw6G038940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 970
AasI GACNNNNNNGTC 1 cut(s) 100
Acc36I ACCTGC 1 cut(s) 970
Acc65I GGTACC 1 cut(s) 441
AccB1I GGYRCC 3 cut(s) 251, 338, 441
AccB7I CCANNNNNTGG 1 cut(s) 745
AccII CGCG 5 cut(s) 80, 195, 215, 239, 241
AciI CCGC 7 cut(s) 75, 80, 134, 207, 249, 413, 883
AclWI GGATC 2 cut(s) 620, 756
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 1 cut(s) 828
AcuI CTGAAG 1 cut(s) 242
AcyI GRCGYC 3 cut(s) 111, 297, 315
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 9 cut(s) 71, 74, 226, 277, 335, 348, 461, 745, 800
AgsI TTSAA 3 cut(s) 190, 492, 721
AjnI CCWGG 3 cut(s) 26, 360, 454
AluBI AGCT 3 cut(s) 34, 842, 850
AluI AGCT 3 cut(s) 34, 842, 850
Alw21I GWGCWC 2 cut(s) 36, 684
Alw26I GTCTC 3 cut(s) 338, 804, 879
Alw44I GTGCAC 1 cut(s) 680
AlwI GGATC 2 cut(s) 620, 756
AlwNI CAGNNNCTG 1 cut(s) 563
Ama87I CYCGRG 1 cut(s) 35
AoxI GGCC 3 cut(s) 105, 268, 363
ApaLI GTGCAC 1 cut(s) 680
ApoI RAATTY 1 cut(s) 828
Asp718I GGTACC 1 cut(s) 441
AspLEI GCGC 6 cut(s) 80, 139, 195, 217, 241, 243
AspS9I GGNCC 3 cut(s) 97, 268, 890
AsuHPI GGTGA 3 cut(s) 443, 783, 784
AvaI CYCGRG 1 cut(s) 35
AvaII GGWCC 2 cut(s) 97, 890
BaeGI GKGCMC 2 cut(s) 341, 684
BanI GGYRCC 3 cut(s) 251, 338, 441
BanII GRGCYC 2 cut(s) 36, 525
BbsI GAAGAC 1 cut(s) 98
Bbv12I GWGCWC 2 cut(s) 36, 684
BccI CCATC 7 cut(s) 49, 122, 220, 263, 308, 432, 638
BceAI ACGGC 2 cut(s) 148, 307
BciT130I CCWGG 3 cut(s) 28, 362, 456
BcoDI GTCTC 3 cut(s) 338, 804, 879
BfaI CTAG 2 cut(s) 348, 951
BfuAI ACCTGC 1 cut(s) 970
BglII AGATCT 1 cut(s) 201
BisI GCNGC 4 cut(s) 76, 81, 135, 250
BlsI GCNGC 4 cut(s) 77, 82, 136, 251
Bme1390I CCNGG 3 cut(s) 28, 362, 456
Bme18I GGWCC 2 cut(s) 97, 890
BmeT110I CYCGRG 1 cut(s) 35
BmgT120I GGNCC 3 cut(s) 97, 268, 890
BmiI GGNNCC 5 cut(s) 99, 253, 340, 443, 522
BmrFI CCNGG 3 cut(s) 28, 362, 456
BmsI GCATC 2 cut(s) 358, 455
BoxI GACNNNNGTC 1 cut(s) 350
BpiI GAAGAC 1 cut(s) 98
BpmI CTGGAG 3 cut(s) 443, 603, 872
BsaHI GRCGYC 3 cut(s) 111, 297, 315
BsaJI CCNNGG 4 cut(s) 27, 164, 360, 454
BsaXI ACNNNNNCTCC 8 cut(s) 149, 179, 201, 227, 231, 257, 801, 831
Bsc4I CCNNNNNNNGG 9 cut(s) 71, 74, 226, 277, 335, 348, 461, 745, 800
Bse118I RCCGGY 1 cut(s) 328
Bse1I ACTGG 1 cut(s) 426
BseBI CCWGG 3 cut(s) 28, 362, 456
BseDI CCNNGG 4 cut(s) 27, 164, 360, 454
BseGI GGATG 4 cut(s) 10, 443, 470, 928
BseLI CCNNNNNNNGG 9 cut(s) 71, 74, 226, 277, 335, 348, 461, 745, 800
BseMII CTCAG 1 cut(s) 509
BseNI ACTGG 1 cut(s) 426
BsePI GCGCGC 1 cut(s) 239
BseSI GKGCMC 2 cut(s) 341, 684
BseX3I CGGCCG 1 cut(s) 105
Bsh1236I CGCG 5 cut(s) 80, 195, 215, 239, 241
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 3 cut(s) 107, 270, 365
BshNI GGYRCC 3 cut(s) 251, 338, 441
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 2 cut(s) 36, 684
BsiHKCI CYCGRG 1 cut(s) 35
BsiSI CCGG 3 cut(s) 72, 246, 329
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 9 cut(s) 71, 74, 226, 277, 335, 348, 461, 745, 800
BsmAI GTCTC 3 cut(s) 338, 804, 879
BsmFI GGGAC 1 cut(s) 35
BsmI GAATGC 1 cut(s) 587
BsnI GGCC 3 cut(s) 107, 270, 365
BsoBI CYCGRG 1 cut(s) 35
Bsp1286I GDGCHC 4 cut(s) 36, 341, 525, 684
Bsp143I GATC 3 cut(s) 201, 625, 748
BspACI CCGC 7 cut(s) 75, 80, 134, 207, 249, 413, 883
BspANI GGCC 3 cut(s) 107, 270, 365
BspCNI CTCAG 1 cut(s) 508
BspFNI CGCG 5 cut(s) 80, 195, 215, 239, 241
BspLI GGNNCC 5 cut(s) 99, 253, 340, 443, 522
BspMI ACCTGC 1 cut(s) 970
BspPI GGATC 2 cut(s) 620, 756
BspT107I GGYRCC 3 cut(s) 251, 338, 441
BsrFI RCCGGY 1 cut(s) 328
BsrI ACTGG 1 cut(s) 426
BssAI RCCGGY 1 cut(s) 328
BssECI CCNNGG 4 cut(s) 27, 164, 360, 454
BssHII GCGCGC 1 cut(s) 239
BssMI GATC 3 cut(s) 201, 625, 748
BssNI GRCGYC 3 cut(s) 111, 297, 315
Bst2UI CCWGG 3 cut(s) 28, 362, 456
Bst4CI ACNGT 4 cut(s) 559, 686, 693, 973
Bst6I CTCTTC 1 cut(s) 790
BstACI GRCGYC 3 cut(s) 111, 297, 315
BstC8I GCNNGC 3 cut(s) 241, 470, 858
BstDEI CTNAG 1 cut(s) 495
BstDSI CCRYGG 1 cut(s) 164
BstENI CCTNNNNNAGG 1 cut(s) 275
BstF5I GGATG 4 cut(s) 10, 443, 470, 928
BstFNI CGCG 5 cut(s) 80, 195, 215, 239, 241
BstHHI GCGC 6 cut(s) 80, 139, 195, 217, 241, 243
BstKTI GATC 3 cut(s) 204, 628, 751
BstMAI GTCTC 3 cut(s) 338, 804, 879
BstMBI GATC 3 cut(s) 201, 625, 748
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 3 cut(s) 14, 160, 249
BstNI CCWGG 3 cut(s) 28, 362, 456
BstPAI GACNNNNGTC 1 cut(s) 350
BstSCI CCNGG 3 cut(s) 26, 360, 454
BstSLI GKGCMC 2 cut(s) 341, 684
BstUI CGCG 5 cut(s) 80, 195, 215, 239, 241
BstV2I GAAGAC 1 cut(s) 98
BstX2I RGATCY 2 cut(s) 201, 748
BstYI RGATCY 2 cut(s) 201, 748
BstZI CGGCCG 1 cut(s) 105
BsuRI GGCC 3 cut(s) 107, 270, 365
BtgI CCRYGG 1 cut(s) 164
BtsCI GGATG 4 cut(s) 10, 443, 470, 928
BtsIMutI CAGTG 2 cut(s) 564, 682
BveI ACCTGC 1 cut(s) 970
Cac8I GCNNGC 3 cut(s) 241, 470, 858
CaiI CAGNNNCTG 1 cut(s) 563
CfoI GCGC 6 cut(s) 80, 139, 195, 217, 241, 243
Cfr10I RCCGGY 1 cut(s) 328
Cfr13I GGNCC 3 cut(s) 97, 268, 890
CseI GACGC 4 cut(s) 119, 184, 305, 323
Csp6I GTAC 1 cut(s) 442
CviAII CATG 4 cut(s) 265, 274, 310, 986
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 1 cut(s) 495
DpnI GATC 3 cut(s) 203, 627, 750
DpnII GATC 3 cut(s) 201, 625, 748
DrdI GACNNNNNNGTC 1 cut(s) 100
DseDI GACNNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 105
EagI CGGCCG 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 790
EarI CTCTTC 1 cut(s) 790
Ecl136II GAGCTC 1 cut(s) 34
EclXI CGGCCG 1 cut(s) 105
Eco24I GRGCYC 2 cut(s) 36, 525
Eco32I GATATC 1 cut(s) 709
Eco47I GGWCC 2 cut(s) 97, 890
Eco52I CGGCCG 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 242
Eco88I CYCGRG 1 cut(s) 35
EcoICRI GAGCTC 1 cut(s) 34
EcoNI CCTNNNNNAGG 1 cut(s) 275
EcoRII CCWGG 3 cut(s) 26, 360, 454
EcoRV GATATC 1 cut(s) 709
EcoT38I GRGCYC 2 cut(s) 36, 525
FaeI CATG 4 cut(s) 268, 277, 313, 989
FaqI GGGAC 1 cut(s) 35
FatI CATG 4 cut(s) 264, 273, 309, 985
FauI CCCGC 2 cut(s) 406, 890
Fnu4HI GCNGC 4 cut(s) 76, 81, 135, 250
FokI GGATG 4 cut(s) 17, 450, 477, 935
FriOI GRGCYC 2 cut(s) 36, 525
Fsp4HI GCNGC 4 cut(s) 76, 81, 135, 250
FspBI CTAG 2 cut(s) 348, 951
GlaI GCGC 6 cut(s) 79, 138, 194, 216, 240, 242
GluI GCNGC 4 cut(s) 76, 81, 135, 250
GsuI CTGGAG 3 cut(s) 443, 603, 872
HaeIII GGCC 3 cut(s) 107, 270, 365
HapII CCGG 3 cut(s) 72, 246, 329
HgaI GACGC 4 cut(s) 119, 184, 305, 323
HhaI GCGC 6 cut(s) 80, 139, 195, 217, 241, 243
Hin1I GRCGYC 3 cut(s) 111, 297, 315
Hin1II CATG 4 cut(s) 268, 277, 313, 989
Hin6I GCGC 6 cut(s) 78, 137, 193, 215, 239, 241
HinP1I GCGC 6 cut(s) 78, 137, 193, 215, 239, 241
HincII GTYRAC 2 cut(s) 172, 577
HindII GTYRAC 2 cut(s) 172, 577
HinfI GANTC 3 cut(s) 734, 865, 929
HpaI GTTAAC 1 cut(s) 172
HpaII CCGG 3 cut(s) 72, 246, 329
HphI GGTGA 3 cut(s) 443, 783, 784
Hpy166II GTNNAC 5 cut(s) 172, 235, 577, 682, 689
Hpy188I TCNGA 6 cut(s) 498, 555, 637, 645, 835, 993
Hpy8I GTNNAC 5 cut(s) 172, 235, 577, 682, 689
Hpy99I CGWCG 5 cut(s) 106, 113, 131, 293, 299
HpyAV CCTTC 2 cut(s) 535, 913
HpyCH4III ACNGT 4 cut(s) 559, 686, 693, 973
HpyCH4V TGCA 6 cut(s) 277, 587, 682, 821, 962, 985
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 160, 249
HpyF3I CTNAG 1 cut(s) 495
Hsp92I GRCGYC 3 cut(s) 111, 297, 315
Hsp92II CATG 4 cut(s) 268, 277, 313, 989
HspAI GCGC 6 cut(s) 78, 137, 193, 215, 239, 241
KpnI GGTACC 1 cut(s) 445
KspAI GTTAAC 1 cut(s) 172
Kzo9I GATC 3 cut(s) 201, 625, 748
LmnI GCTCC 6 cut(s) 31, 222, 248, 520, 853, 976
LweI GCATC 2 cut(s) 358, 455
MaeI CTAG 2 cut(s) 348, 951
MaeIII GTNAC 2 cut(s) 898, 996
MalI GATC 3 cut(s) 203, 627, 750
MboI GATC 3 cut(s) 201, 625, 748
MboII GAAGA 6 cut(s) 103, 109, 178, 235, 777, 938
MflI RGATCY 2 cut(s) 201, 748
MhlI GDGCHC 4 cut(s) 36, 341, 525, 684
MluCI AATT 4 cut(s) 512, 663, 828, 945
MlyI GAGTC 1 cut(s) 728
MmeI TCCRAC 1 cut(s) 415
MseI TTAA 3 cut(s) 171, 503, 657
MslI CAYNNNNRTG 1 cut(s) 308
MspI CCGG 3 cut(s) 72, 246, 329
MspR9I CCNGG 3 cut(s) 28, 362, 456
Mva1269I GAATGC 1 cut(s) 587
MvaI CCWGG 3 cut(s) 28, 362, 456
MvnI CGCG 5 cut(s) 80, 195, 215, 239, 241
MwoI GCNNNNNNNGC 3 cut(s) 14, 160, 249
NdeII GATC 3 cut(s) 201, 625, 748
NlaIII CATG 4 cut(s) 268, 277, 313, 989
NlaIV GGNNCC 5 cut(s) 99, 253, 340, 443, 522
NmuCI GTSAC 1 cut(s) 996
PaeR7I CTCGAG 1 cut(s) 35
PaqCI CACCTGC 1 cut(s) 970
PauI GCGCGC 1 cut(s) 239
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PctI GAATGC 1 cut(s) 587
PfeI GAWTC 2 cut(s) 865, 929
PflFI GACNNNGTC 1 cut(s) 559
PflMI CCANNNNNTGG 1 cut(s) 745
PkrI GCNGC 4 cut(s) 77, 82, 136, 251
PleI GAGTC 1 cut(s) 728
PpsI GAGTC 1 cut(s) 728
PshAI GACNNNNGTC 1 cut(s) 350
Psp124BI GAGCTC 1 cut(s) 36
Psp6I CCWGG 3 cut(s) 26, 360, 454
PspGI CCWGG 3 cut(s) 26, 360, 454
PspN4I GGNNCC 5 cut(s) 99, 253, 340, 443, 522
PspPI GGNCC 3 cut(s) 97, 268, 890
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 2 cut(s) 201, 748
PsyI GACNNNGTC 1 cut(s) 559
PteI GCGCGC 1 cut(s) 239
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
RseI CAYNNNNRTG 1 cut(s) 308
SacI GAGCTC 1 cut(s) 36
SaqAI TTAA 3 cut(s) 171, 503, 657
SatI GCNGC 4 cut(s) 76, 81, 135, 250
Sau3AI GATC 3 cut(s) 201, 625, 748
Sau96I GGNCC 3 cut(s) 97, 268, 890
SchI GAGTC 1 cut(s) 728
ScrFI CCNGG 3 cut(s) 28, 362, 456
SduI GDGCHC 4 cut(s) 36, 341, 525, 684
SetI ASST 9 cut(s) 29, 36, 264, 744, 796, 844, 852, 984, 1005
SfaNI GCATC 2 cut(s) 358, 455
Sfr274I CTCGAG 1 cut(s) 35
SinI GGWCC 2 cut(s) 97, 890
SlaI CTCGAG 1 cut(s) 35
SmiMI CAYNNNNRTG 1 cut(s) 308
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
Sse9I AATT 4 cut(s) 512, 663, 828, 945
SsiI CCGC 7 cut(s) 75, 80, 134, 207, 249, 413, 883
SspMI CTAG 2 cut(s) 348, 951
SstI GAGCTC 1 cut(s) 36
StyD4I CCNGG 3 cut(s) 26, 360, 454
TaaI ACNGT 4 cut(s) 559, 686, 693, 973
TaqI TCGA 4 cut(s) 36, 258, 387, 543
TaqII GACCGA 1 cut(s) 302
TasI AATT 4 cut(s) 512, 663, 828, 945
TauI GCSGC 4 cut(s) 78, 83, 137, 252
TfiI GAWTC 2 cut(s) 865, 929
Tru1I TTAA 3 cut(s) 171, 503, 657
Tru9I TTAA 3 cut(s) 171, 503, 657
TscAI CASTG 2 cut(s) 564, 689
TseFI GTSAC 1 cut(s) 996
Tsp45I GTSAC 1 cut(s) 996
TspDTI ATGAA 1 cut(s) 939
TspGWI ACGGA 2 cut(s) 57, 110
TspRI CASTG 2 cut(s) 564, 689
Tth111I GACNNNGTC 1 cut(s) 559
Van91I CCANNNNNTGG 1 cut(s) 745
VneI GTGCAC 1 cut(s) 680
VpaK11BI GGWCC 2 cut(s) 97, 890
XagI CCTNNNNNAGG 1 cut(s) 275
XapI RAATTY 1 cut(s) 828
XcmI CCANNNNNNNNNTGG 3 cut(s) 262, 307, 452
XhoI CTCGAG 1 cut(s) 35
XspI CTAG 2 cut(s) 348, 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.