AT1G25510

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014902
Physical Location & Seq
Reverse (-)
8959087 .. 8961183
2097 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G25510.1

Sequence Viewer

Length: 1452 bp
ATGTCTCCTAACTACTCCTTCTTCTTCTTCATCTTCTTCCTCACTTCACACTCTTCTGTTTTCTCCAGAATCTTACCAGAGACCTCCACAACCACTACCTCAATTCTCAATGTCGCTGACTCCATCCACAGAACCAAGTATACGTCGTCGTTTCGGTTAAACCAGCAAGAAGAACAGACTCACTCTGCTTCTTCTTCTTTCAGCTTGCAGCTTCATTCACGAGTTTCCGTCCGAGGAACTGAACACTCAGACTATAAGTCACTCACACTAGCCAGACTCAACCGTGACACAGCCCGAGTCAAATCTCTCATAACCCGTTTAGATCTAGCCATCAACAACATATCCAAAGCAGATCTCAAACCCATCAGCACAATGTACACCACTGAGGAACAAGATATCGAAGCTCCTTTAATCTCCGGCACGACTCAAGGAAGTGGCGAGTACTTCACACGCGTCGGTATCGGAAAACCGGCGCGTGAAGTTTATATGGTTCTAGATACTGGAAGTGACGTGAACTGGCTACAATGTACTCCTTGCGCTGACTGTTACCACCAAACCGAACCAATCTTCGAGCCATCGTCGTCTTCTTCTTACGAGCCTCTCTCTTGTGACACTCCACAGTGCAATGCCCTTGAAGTCTCAGAATGTAGAAACGCCACGTGTCTTTACGAGGTTTCTTACGGTGATGGTTCTTACACCGTTGGAGATTTCGCCACCGAGACTCTCACAATCGGCTCAACTCTAGTTCAAAACGTCGCCGTTGGTTGTGGACACAGCAACGAAGGGTTGTTCGTCGGCGCCGCCGGTTTACTTGGACTCGGCGGTGGTTTGTTAGCTCTTCCGTCGCAGCTCAACACGACGTCGTTCTCGTACTGTCTCGTTGACCGTGACTCAGACTCGGCTTCGACCGTGGATTTCGGGACTTCTCTTTCTCCTGACGCCGTCGTGGCTCCGTTGCTACGTAACCACCAGCTCGACACGTTTTACTACCTAGGCCTCACCGGAATAAGCGTCGGCGGCGAGTTGCTTCAGATTCCTCAGTCTTCTTTCGAGATGGACGAGTCAGGCAGCGGTGGGATCATCATCGACTCAGGAACCGCCGTGACTCGTCTCCAGACCGAAATCTATAACTCGCTTAGAGACTCGTTCGTGAAAGGAACGTTGGACTTGGAAAAAGCAGCCGGGGTGGCGATGTTTGACACGTGTTATAACCTTTCGGCTAAGACGACGGTAGAAGTCCCGACCGTGGCTTTTCATTTCCCCGGCGGGAAGATGTTGGCTTTGCCGGCGAAGAACTACATGATTCCGGTTGACTCGGTGGGGACATTTTGTTTGGCATTTGCACCGACGGCGAGTTCACTAGCGATTATCGGAAACGTGCAGCAACAAGGGACACGTGTCACTTTCGATTTGGCAAATTCTCTCATTGGATTCTCGTCTAATAAATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

483

Amino Acids

51.99

Weight (kDa)

4.82

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 147 - 479 7.8e-21 Eukaryotic aspartyl protease
TAXi_N PF14543 148 - 307 2.5e-48 Xylanase inhibitor N-terminal
TAXi_C PF14541 329 - 479 1.8e-38 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014902)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25510
fragaria_vesca FvH4_4g27770
malus_domestica MD13G1044900.v1.1
prunus_persica Prupe.1G307100_v2.0.a1
pyrus_communis pycom13g03970 pycom16g04080
rosa_chinensis RchiOBHm_Chr4g0435631
rosa_laevigata RLG00000006575
rosa_multiflora Rmu_sc0000051.1_g000004
rosa_roxburghii Rroxscaffold_5G00376850
rosa_rugosa Rorug04G0287400
rosa_samantha Rh4AG342600 Rh4BG350900 Rh4CG365600 Rh4DG345300
rosa_wichuraiana Rw4G029900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1209
AatII GACGTC 1 cut(s) 863
AccB1I GGYRCC 1 cut(s) 797
AccI GTMKAC 1 cut(s) 140
AccII CGCG 2 cut(s) 453, 475
AciI CCGC 6 cut(s) 801, 822, 1017, 1071, 1098, 1266
AclI AACGTT 1 cut(s) 1160
AclWI GGATC 1 cut(s) 1085
AcsI RAATTY 1 cut(s) 1417
AcuI CTGAAG 1 cut(s) 1013
AcvI CACGTG 3 cut(s) 660, 1203, 1397
AcyI GRCGYC 3 cut(s) 798, 860, 939
AfaI GTAC 4 cut(s) 377, 443, 529, 872
AfiI CCNNNNNNNGG 1 cut(s) 1246
AflIII ACRYGT 7 cut(s) 451, 659, 978, 1200, 1202, 1394, 1396
AgsI TTSAA 2 cut(s) 635, 749
AhdI GACNNNNNGTC 1 cut(s) 256
AjiI CACGTC 1 cut(s) 511
AjuI GAANNNNNNNTTGG 2 cut(s) 1145, 1177
AluBI AGCT 6 cut(s) 204, 211, 404, 836, 850, 973
AluI AGCT 6 cut(s) 204, 211, 404, 836, 850, 973
Alw26I GTCTC 7 cut(s) 9, 74, 643, 713, 881, 1115, 1134
AlwI GGATC 1 cut(s) 1085
Ama87I CYCGRG 1 cut(s) 294
AoxI GGCC 1 cut(s) 994
ApeKI GCWGC 5 cut(s) 208, 847, 1068, 1178, 1381
ApoI RAATTY 1 cut(s) 1417
ArsI GACNNNNNNTTYG 2 cut(s) 1315, 1347
AspA2I CCTAGG 1 cut(s) 991
AspLEI GCGC 3 cut(s) 475, 539, 800
AsuC2I CCSGG 2 cut(s) 1183, 1263
AsuHPI GGTGA 2 cut(s) 695, 991
AvaI CYCGRG 1 cut(s) 294
AvrII CCTAGG 1 cut(s) 991
BanI GGYRCC 1 cut(s) 797
BauI CACGAG 1 cut(s) 219
BbrPI CACGTG 3 cut(s) 660, 1203, 1397
BbsI GAAGAC 2 cut(s) 576, 1035
BbvI GCAGC 5 cut(s) 220, 859, 1080, 1190, 1393
BccI CCATC 6 cut(s) 131, 338, 371, 583, 680, 1048
BceAI ACGGC 4 cut(s) 743, 926, 1085, 1365
BcnI CCSGG 2 cut(s) 1183, 1263
BcoDI GTCTC 7 cut(s) 9, 74, 643, 713, 881, 1115, 1134
BfaI CTAG 6 cut(s) 269, 326, 494, 743, 992, 1361
BfoI RGCGCY 1 cut(s) 801
BglI GCCNNNNNGGC 2 cut(s) 947, 1187
BglII AGATCT 2 cut(s) 322, 352
BisI GCNGC 7 cut(s) 209, 801, 848, 1018, 1069, 1179, 1382
BlnI CCTAGG 1 cut(s) 991
BlsI GCNGC 7 cut(s) 210, 802, 849, 1019, 1070, 1180, 1383
BmcAI AGTACT 1 cut(s) 443
Bme1390I CCNGG 2 cut(s) 1183, 1263
BmeRI GACNNNNNGTC 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 294
BmgBI CACGTC 1 cut(s) 511
BmiI GGNNCC 3 cut(s) 799, 951, 1096
BmrFI CCNGG 2 cut(s) 1183, 1263
BoxI GACNNNNGTC 1 cut(s) 1397
BpiI GAAGAC 2 cut(s) 576, 1035
BplI GAGNNNNNCTC 2 cut(s) 587, 619
BpmI CTGGAG 2 cut(s) 49, 1097
BpuEI CTTGAG 1 cut(s) 411
BpuMI CCSGG 2 cut(s) 1183, 1263
BsaAI YACGTR 4 cut(s) 660, 962, 1203, 1397
BsaBI GATNNNNATC 1 cut(s) 1082
BsaHI GRCGYC 3 cut(s) 798, 860, 939
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 6 cut(s) 232, 909, 991, 1182, 1245, 1261
BsaWI WCCGGW 2 cut(s) 1001, 1306
Bsc4I CCNNNNNNNGG 1 cut(s) 1246
Bse118I RCCGGY 3 cut(s) 469, 803, 1285
Bse1I ACTGG 2 cut(s) 505, 521
Bse3DI GCAATG 1 cut(s) 631
Bse8I GATNNNNATC 1 cut(s) 1082
BseDI CCNNGG 6 cut(s) 232, 909, 991, 1182, 1245, 1261
BseGI GGATG 1 cut(s) 123
BseJI GATNNNNATC 1 cut(s) 1082
BseLI CCNNNNNNNGG 1 cut(s) 1246
BseMI GCAATG 1 cut(s) 631
BseMII CTCAG 6 cut(s) 261, 375, 654, 906, 1052, 1104
BseNI ACTGG 2 cut(s) 505, 521
BseXI GCAGC 5 cut(s) 220, 859, 1080, 1190, 1393
BsgI GTGCAG 1 cut(s) 1400
Bsh1236I CGCG 2 cut(s) 453, 475
Bsh1285I CGRYCG 2 cut(s) 909, 1245
BshFI GGCC 1 cut(s) 996
BshNI GGYRCC 1 cut(s) 797
BsiEI CGRYCG 2 cut(s) 909, 1245
BsiHKCI CYCGRG 1 cut(s) 294
BsiSI CCGG 8 cut(s) 417, 470, 804, 1002, 1182, 1263, 1286, 1307
BslFI GGGAC 4 cut(s) 934, 1223, 1336, 1405
BslI CCNNNNNNNGG 1 cut(s) 1246
BsmAI GTCTC 7 cut(s) 9, 74, 643, 713, 881, 1115, 1134
BsmBI CGTCTC 1 cut(s) 1115
BsmFI GGGAC 4 cut(s) 934, 1223, 1336, 1405
BsnI GGCC 1 cut(s) 996
Bso31I GGTCTC 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 294
Bsp1407I TGTACA 1 cut(s) 375
Bsp143I GATC 3 cut(s) 322, 352, 1077
BspACI CCGC 6 cut(s) 801, 822, 1017, 1071, 1098, 1266
BspANI GGCC 1 cut(s) 996
BspCNI CTCAG 6 cut(s) 260, 376, 653, 905, 1051, 1103
BspFNI CGCG 2 cut(s) 453, 475
BspLI GGNNCC 3 cut(s) 799, 951, 1096
BspPI GGATC 1 cut(s) 1085
BspQI GCTCTTC 1 cut(s) 843
BspT107I GGYRCC 1 cut(s) 797
BspTNI GGTCTC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 631
BsrFI RCCGGY 3 cut(s) 469, 803, 1285
BsrGI TGTACA 1 cut(s) 375
BsrI ACTGG 2 cut(s) 505, 521
BssAI RCCGGY 3 cut(s) 469, 803, 1285
BssECI CCNNGG 6 cut(s) 232, 909, 991, 1182, 1245, 1261
BssMI GATC 3 cut(s) 322, 352, 1077
BssNAI GTATAC 1 cut(s) 141
BssNI GRCGYC 3 cut(s) 798, 860, 939
BssSI CACGAG 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 991
Bst1107I GTATAC 1 cut(s) 141
Bst2BI CACGAG 1 cut(s) 219
Bst6I CTCTTC 2 cut(s) 58, 843
BstACI GRCGYC 3 cut(s) 798, 860, 939
BstAUI TGTACA 1 cut(s) 375
BstBAI YACGTR 4 cut(s) 660, 962, 1203, 1397
BstC8I GCNNGC 2 cut(s) 206, 1287
BstDEI CTNAG 8 cut(s) 247, 384, 640, 892, 1038, 1090, 1136, 1221
BstDSI CCRYGG 2 cut(s) 909, 1245
BstF5I GGATG 1 cut(s) 123
BstFNI CGCG 2 cut(s) 453, 475
BstH2I RGCGCY 1 cut(s) 801
BstHHI GCGC 3 cut(s) 475, 539, 800
BstKTI GATC 3 cut(s) 325, 355, 1080
BstMAI GTCTC 7 cut(s) 9, 74, 643, 713, 881, 1115, 1134
BstMBI GATC 3 cut(s) 322, 352, 1077
BstMCI CGRYCG 2 cut(s) 909, 1245
BstMWI GCNNNNNNNGC 5 cut(s) 947, 1017, 1187, 1286, 1349
BstPAI GACNNNNGTC 1 cut(s) 1397
BstSCI CCNGG 2 cut(s) 1181, 1261
BstSNI TACGTA 1 cut(s) 962
BstUI CGCG 2 cut(s) 453, 475
BstV1I GCAGC 5 cut(s) 220, 859, 1080, 1190, 1393
BstV2I GAAGAC 2 cut(s) 576, 1035
BstX2I RGATCY 2 cut(s) 322, 352
BstYI RGATCY 2 cut(s) 322, 352
BstZ17I GTATAC 1 cut(s) 141
BsuRI GGCC 1 cut(s) 996
BtgI CCRYGG 2 cut(s) 909, 1245
BtgZI GCGATG 1 cut(s) 1205
BtrI CACGTC 1 cut(s) 511
BtsCI GGATG 1 cut(s) 123
BtsIMutI CAGTG 2 cut(s) 381, 626
Cac8I GCNNGC 2 cut(s) 206, 1287
CfoI GCGC 3 cut(s) 475, 539, 800
Cfr10I RCCGGY 3 cut(s) 469, 803, 1285
CseI GACGC 3 cut(s) 442, 947, 1000
Csp6I GTAC 4 cut(s) 376, 442, 528, 871
CviAII CATG 1 cut(s) 1300
CviQI GTAC 4 cut(s) 376, 442, 528, 871
DdeI CTNAG 8 cut(s) 247, 384, 640, 892, 1038, 1090, 1136, 1221
DinI GGCGCC 1 cut(s) 799
DpnI GATC 3 cut(s) 324, 354, 1079
DpnII GATC 3 cut(s) 322, 352, 1077
DriI GACNNNNNGTC 1 cut(s) 256
Eam1104I CTCTTC 2 cut(s) 58, 843
Eam1105I GACNNNNNGTC 1 cut(s) 256
EarI CTCTTC 2 cut(s) 58, 843
Eco105I TACGTA 1 cut(s) 962
Eco130I CCWWGG 1 cut(s) 991
Eco147I AGGCCT 1 cut(s) 996
Eco31I GGTCTC 1 cut(s) 74
Eco32I GATATC 1 cut(s) 397
Eco57I CTGAAG 1 cut(s) 1013
Eco72I CACGTG 3 cut(s) 660, 1203, 1397
Eco88I CYCGRG 1 cut(s) 294
EcoRV GATATC 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 991
EgeI GGCGCC 1 cut(s) 799
EheI GGCGCC 1 cut(s) 799
ErhI CCWWGG 1 cut(s) 991
Esp3I CGTCTC 1 cut(s) 1115
FaeI CATG 1 cut(s) 1303
FaiI YATR 9 cut(s) 141, 255, 311, 341, 486, 488, 1128, 1209, 1301
FaqI GGGAC 4 cut(s) 934, 1223, 1336, 1405
FatI CATG 1 cut(s) 1299
FauI CCCGC 1 cut(s) 1259
FblI GTMKAC 1 cut(s) 140
Fnu4HI GCNGC 7 cut(s) 209, 801, 848, 1018, 1069, 1179, 1382
FokI GGATG 1 cut(s) 110
Fsp4HI GCNGC 7 cut(s) 209, 801, 848, 1018, 1069, 1179, 1382
FspBI CTAG 6 cut(s) 269, 326, 494, 743, 992, 1361
GlaI GCGC 3 cut(s) 474, 538, 799
GluI GCNGC 7 cut(s) 209, 801, 848, 1018, 1069, 1179, 1382
GsuI CTGGAG 2 cut(s) 49, 1097
HaeII RGCGCY 1 cut(s) 801
HaeIII GGCC 1 cut(s) 996
HapII CCGG 8 cut(s) 417, 470, 804, 1002, 1182, 1263, 1286, 1307
HgaI GACGC 3 cut(s) 442, 947, 1000
HhaI GCGC 3 cut(s) 475, 539, 800
Hin1I GRCGYC 3 cut(s) 798, 860, 939
Hin1II CATG 1 cut(s) 1303
Hin6I GCGC 3 cut(s) 473, 537, 798
HinP1I GCGC 3 cut(s) 473, 537, 798
HincII GTYRAC 2 cut(s) 883, 1312
HindII GTYRAC 2 cut(s) 883, 1312
HpaII CCGG 8 cut(s) 417, 470, 804, 1002, 1182, 1263, 1286, 1307
HphI GGTGA 2 cut(s) 695, 991
Hpy166II GTNNAC 8 cut(s) 141, 378, 514, 770, 809, 883, 1312, 1358
Hpy188I TCNGA 7 cut(s) 233, 250, 464, 643, 895, 1032, 1373
Hpy8I GTNNAC 8 cut(s) 141, 378, 514, 770, 809, 883, 1312, 1358
HpyAV CCTTC 2 cut(s) 28, 776
HpyCH4V TGCA 4 cut(s) 208, 624, 1343, 1381
HpyF10VI GCNNNNNNNGC 5 cut(s) 947, 1017, 1187, 1286, 1349
HpyF3I CTNAG 8 cut(s) 247, 384, 640, 892, 1038, 1090, 1136, 1221
Hsp92I GRCGYC 3 cut(s) 798, 860, 939
Hsp92II CATG 1 cut(s) 1303
HspAI GCGC 3 cut(s) 473, 537, 798
KasI GGCGCC 1 cut(s) 797
KroI GCCGGC 1 cut(s) 1285
KroNI GCCGGC 1 cut(s) 1287
Kzo9I GATC 3 cut(s) 322, 352, 1077
LguI GCTCTTC 1 cut(s) 843
LmnI GCTCC 2 cut(s) 409, 955
Lsp1109I GCAGC 5 cut(s) 220, 859, 1080, 1190, 1393
MaeI CTAG 6 cut(s) 269, 326, 494, 743, 992, 1361
MaeIII GTNAC 9 cut(s) 258, 284, 506, 545, 608, 887, 962, 1102, 1399
MalI GATC 3 cut(s) 324, 354, 1079
MboI GATC 3 cut(s) 322, 352, 1077
MflI RGATCY 2 cut(s) 322, 352
MluCI AATT 2 cut(s) 102, 1417
MluI ACGCGT 1 cut(s) 451
Mly113I GGCGCC 1 cut(s) 798
MmeI TCCRAC 2 cut(s) 682, 1143
MnlI CCTC 9 cut(s) 50, 94, 109, 227, 379, 609, 664, 1007, 1047
MroNI GCCGGC 1 cut(s) 1285
MseI TTAA 2 cut(s) 158, 410
MspA1I CMGCKG 1 cut(s) 1071
MspI CCGG 8 cut(s) 417, 470, 804, 1002, 1182, 1263, 1286, 1307
MspR9I CCNGG 2 cut(s) 1183, 1263
MvnI CGCG 2 cut(s) 453, 475
MwoI GCNNNNNNNGC 5 cut(s) 947, 1017, 1187, 1286, 1349
NaeI GCCGGC 1 cut(s) 1287
NarI GGCGCC 1 cut(s) 798
NciI CCSGG 2 cut(s) 1183, 1263
NdeII GATC 3 cut(s) 322, 352, 1077
NgoMIV GCCGGC 1 cut(s) 1285
NlaIII CATG 1 cut(s) 1303
NlaIV GGNNCC 3 cut(s) 799, 951, 1096
NmeAIII GCCGAG 2 cut(s) 798, 878
NmuCI GTSAC 7 cut(s) 258, 284, 506, 608, 887, 1102, 1399
PceI AGGCCT 1 cut(s) 996
PciSI GCTCTTC 1 cut(s) 843
PcsI WCGNNNNNNNCGW 3 cut(s) 866, 1056, 1223
PdiI GCCGGC 1 cut(s) 1287
PfeI GAWTC 4 cut(s) 69, 1033, 1303, 1431
PflFI GACNNNGTC 1 cut(s) 941
PkrI GCNGC 7 cut(s) 210, 802, 849, 1019, 1070, 1180, 1383
PluTI GGCGCC 1 cut(s) 801
PmaCI CACGTG 3 cut(s) 660, 1203, 1397
PmlI CACGTG 3 cut(s) 660, 1203, 1397
Ppu21I YACGTR 4 cut(s) 660, 962, 1203, 1397
PshAI GACNNNNGTC 1 cut(s) 1397
PsiI TTATAA 1 cut(s) 1209
Psp1406I AACGTT 1 cut(s) 1160
PspCI CACGTG 3 cut(s) 660, 1203, 1397
PspN4I GGNNCC 3 cut(s) 799, 951, 1096
PsrI GAACNNNNNNTAC 2 cut(s) 124, 156
PsuI RGATCY 2 cut(s) 322, 352
PsyI GACNNNGTC 1 cut(s) 941
RsaI GTAC 4 cut(s) 377, 443, 529, 872
RsaNI GTAC 4 cut(s) 376, 442, 528, 871
SapI GCTCTTC 1 cut(s) 843
SaqAI TTAA 2 cut(s) 158, 410
SatI GCNGC 7 cut(s) 209, 801, 848, 1018, 1069, 1179, 1382
Sau3AI GATC 3 cut(s) 322, 352, 1077
ScaI AGTACT 1 cut(s) 443
ScrFI CCNGG 2 cut(s) 1183, 1263
SfoI GGCGCC 1 cut(s) 799
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
SnaBI TACGTA 1 cut(s) 962
Sse9I AATT 2 cut(s) 102, 1417
SseBI AGGCCT 1 cut(s) 996
SsiI CCGC 6 cut(s) 801, 822, 1017, 1071, 1098, 1266
SspDI GGCGCC 1 cut(s) 797
SspMI CTAG 6 cut(s) 269, 326, 494, 743, 992, 1361
StuI AGGCCT 1 cut(s) 996
StyD4I CCNGG 2 cut(s) 1181, 1261
StyI CCWWGG 1 cut(s) 991
TaqI TCGA 7 cut(s) 399, 570, 905, 975, 1050, 1086, 1407
TaqII GACCGA 1 cut(s) 1133
TasI AATT 2 cut(s) 102, 1417
TatI WGTACW 3 cut(s) 375, 441, 527
TauI GCSGC 2 cut(s) 803, 1020
TfiI GAWTC 4 cut(s) 69, 1033, 1303, 1431
Tru1I TTAA 2 cut(s) 158, 410
Tru9I TTAA 2 cut(s) 158, 410
TscAI CASTG 2 cut(s) 388, 626
TseFI GTSAC 7 cut(s) 258, 284, 506, 608, 887, 1102, 1399
TseI GCWGC 5 cut(s) 208, 847, 1068, 1178, 1381
Tsp45I GTSAC 7 cut(s) 258, 284, 506, 608, 887, 1102, 1399
TspDTI ATGAA 3 cut(s) 19, 203, 1244
TspGWI ACGGA 3 cut(s) 217, 831, 942
TspRI CASTG 2 cut(s) 388, 626
Tth111I GACNNNGTC 1 cut(s) 941
XapI RAATTY 1 cut(s) 1417
XbaI TCTAGA 1 cut(s) 493
XmaJI CCTAGG 1 cut(s) 991
XmiI GTMKAC 1 cut(s) 140
XspI CTAG 6 cut(s) 269, 326, 494, 743, 992, 1361
ZraI GACGTC 1 cut(s) 861
ZrmI AGTACT 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.