pycom13g03970

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0014902
Physical Location & Seq
Reverse (-)
2619778 .. 2621244
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g03970.1

Sequence Viewer

Length: 1467 bp
ATGGCCGGAATGGGTTTCCTCTCCTGCATTCTCTTTGCTTTCCTCTTTCACTGTCATTCCCAAGTTGCCCACTCCCGGAGCTCGCCGTCGACCTCCAAAACGACGGTGCTCGACGTCGCCGCTTCGATTCAGACGACGCTAAGAGCTCTGTCGTCGGAGGATACAAGTAGAACGGCACAGGCATTGTCCCAGCAAGACCATAGCTCGCTTTCTGTACCGTTACACTCTCGAATTTCACTTCACAAGCCTTCTCACAGCGACTACAAGTCCCTCACTTTGGCTCGACTCGAGCGCGACTCGGTCCGAGTCAGATCTCTCACCACTCGGTTGGATCTGGCTGTTAGAGGTGTTGCCACGTCGGACCTTAAACCCGTGGAAACCGGCAGCGGGTTACAGCTCGATGCGGATGGTTTTGAGGGCCCCATTATTTCCGGGACGAGTCAGGGTAGCGGCGAGTACTTCTCCCGAGTCGGGATCGGAAAACCGCCGAGTCAGGCCTACGTGGTGCTCGACACCGGGAGTGACATCAGCTGGGTGCAGTGCGCACCCTGCGCCGATTGCTACCAGCAAGCCGACCCTATCTTCGAGCCGGCTTCCTCGACTTCCTTCTCGCCGCTCTCGTGCGAATCCCAACAATGCAAATCCCTCGATGTGTTTGAGTGTCGGAACGGCACGTGTCTCTACGAGGTGGCGTACGGTGACGGCTCGTACACTGTCGGCGACTTTGTGACGGAGACAATCAGCATAGGCGGGGCTTCGGCGAAGGAGATTGCCATCGGCTGTGGCCACACCAACGAGGGCTTGTTCGTCGGAGCGGCTGGGCTTCTCGGACTCGGCGGCGGCTCACTCTCTTTTCCATCCCAGCTCAATGCCACGTCACTTTCCTACTGCCTCGTGGACCGCGACTCCGACTCGGCATCGACCCTCGATTTCAACTCGCCGCTCCGTCCCAACGCCGTTACTGCTCCGTTACGCCGCAACTCTCAGCTGGACACGTTTTACTATCTCGGTCTGACCGGACTCAGCGTCGGTGGCTCATTGCTCCCGATTCCCGAGTCCGCTTTCCAGATAGACGGGAGCGGAAACGGCGGAATCATCATCGACTCCGGCACGGCGGTGACTCGACTGCAGACGGACACATACAACGTGCTTCGCGACGCGTTCATGAAAGGCACTAAGGACTTGCCGTTTACGAAAGGGCCAGCGTTATTCGACGCGTGTTACGACTTGTCATCGAGGAAGAGCGTGGAGGTTCCGACGGTGTCGTTTCACTTCGCCGATGGGAAAGTGTTACCGTTACCGGCCAAAAATTTCCTGATACCGGTCGACTCGGACGGGACGTTTTGTTTTGCGTTCGCGCCCACGCCCTCTCCGCTGTCAATCATTGGGAATGTCCAACAGCAAGGGACACGTGTCGGTTTCGATCTAGTGAATTCTGTGGTCGGGTTCTCCCCCAACCAATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

489

Amino Acids

51.54

Weight (kDa)

5.11

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 152 - 284 2.2e-11 Eukaryotic aspartyl protease
TAXi_N PF14543 153 - 309 3.8e-46 Xylanase inhibitor N-terminal
TAXi_C PF14541 334 - 484 1.1e-35 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014902)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25510
fragaria_vesca FvH4_4g27770
malus_domestica MD13G1044900.v1.1
prunus_persica Prupe.1G307100_v2.0.a1
pyrus_communis pycom13g03970 pycom16g04080
rosa_chinensis RchiOBHm_Chr4g0435631
rosa_laevigata RLG00000006575
rosa_multiflora Rmu_sc0000051.1_g000004
rosa_roxburghii Rroxscaffold_5G00376850
rosa_rugosa Rorug04G0287400
rosa_samantha Rh4AG342600 Rh4BG350900 Rh4CG365600 Rh4DG345300
rosa_wichuraiana Rw4G029900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 117
Acc16I TGCGCA 1 cut(s) 544
AccBSI CCGCTC 4 cut(s) 616, 815, 943, 1080
AccI GTMKAC 2 cut(s) 89, 1326
AccII CGCG 6 cut(s) 294, 903, 1155, 1160, 1217, 1358
AclWI GGATC 2 cut(s) 339, 482
AcoI YGGCCR 3 cut(s) 3, 784, 1302
AcsI RAATTY 3 cut(s) 231, 1309, 1432
AcvI CACGTG 2 cut(s) 675, 1412
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 4 cut(s) 216, 458, 695, 710
AfiI CCNNNNNNNGG 5 cut(s) 75, 277, 387, 471, 1321
AflIII ACRYGT 6 cut(s) 674, 993, 1158, 1215, 1409, 1411
AgeI ACCGGT 1 cut(s) 1321
AgsI TTSAA 1 cut(s) 934
AhdI GACNNNNNGTC 2 cut(s) 265, 1025
AjiI CACGTC 2 cut(s) 357, 876
AleI CACNNNNGTG 1 cut(s) 1115
AluBI AGCT 7 cut(s) 81, 146, 204, 397, 531, 865, 988
AluI AGCT 7 cut(s) 81, 146, 204, 397, 531, 865, 988
Alw21I GWGCWC 4 cut(s) 83, 111, 148, 510
Alw26I GTCTC 2 cut(s) 683, 728
AlwI GGATC 2 cut(s) 339, 482
Ama87I CYCGRG 3 cut(s) 287, 465, 1052
AoxI GGCC 6 cut(s) 3, 418, 495, 784, 1199, 1302
ApaI GGGCCC 1 cut(s) 422
ApeKI GCWGC 1 cut(s) 384
ApoI RAATTY 3 cut(s) 231, 1309, 1432
ArsI GACNNNNNNTTYG 2 cut(s) 566, 598
AsiGI ACCGGT 1 cut(s) 1321
AspLEI GCGC 4 cut(s) 294, 545, 554, 1360
AspS9I GGNCC 6 cut(s) 301, 361, 418, 419, 898, 1199
AsuC2I CCSGG 3 cut(s) 76, 433, 517
AsuHPI GGTGA 3 cut(s) 310, 710, 1129
AvaI CYCGRG 3 cut(s) 287, 465, 1052
AvaII GGWCC 3 cut(s) 301, 361, 898
BaeGI GKGCMC 1 cut(s) 422
BalI TGGCCA 1 cut(s) 786
BanII GRGCYC 3 cut(s) 83, 148, 422
BauI CACGAG 2 cut(s) 619, 893
BbrPI CACGTG 2 cut(s) 675, 1412
Bbv12I GWGCWC 4 cut(s) 83, 111, 148, 510
BbvI GCAGC 1 cut(s) 396
BccI CCATC 4 cut(s) 401, 782, 865, 1274
BceAI ACGGC 8 cut(s) 70, 189, 685, 718, 941, 1102, 1128, 1171
BcgI CGANNNNNNTGC 2 cut(s) 628, 662
BciVI GTATCC 1 cut(s) 154
BcnI CCSGG 3 cut(s) 76, 433, 517
BcoDI GTCTC 2 cut(s) 683, 728
BfaI CTAG 1 cut(s) 1427
BfmI CTRYAG 1 cut(s) 1127
BfuI GTATCC 1 cut(s) 154
BglII AGATCT 1 cut(s) 311
BisI GCNGC 9 cut(s) 120, 385, 451, 614, 816, 838, 841, 941, 976
BlsI GCNGC 9 cut(s) 121, 386, 452, 615, 817, 839, 842, 942, 977
BmcAI AGTACT 1 cut(s) 458
Bme1390I CCNGG 3 cut(s) 76, 433, 517
Bme18I GGWCC 3 cut(s) 301, 361, 898
BmeRI GACNNNNNGTC 2 cut(s) 265, 1025
BmeT110I CYCGRG 3 cut(s) 287, 465, 1052
BmgBI CACGTC 2 cut(s) 357, 876
BmgT120I GGNCC 6 cut(s) 301, 361, 418, 419, 898, 1199
BmiI GGNNCC 3 cut(s) 420, 421, 1254
BmrFI CCNGG 3 cut(s) 76, 433, 517
BmsI GCATC 2 cut(s) 391, 926
BoxI GACNNNNGTC 1 cut(s) 1412
BplI GAGNNNNNCTC 4 cut(s) 281, 313, 446, 478
BpuMI CCSGG 3 cut(s) 76, 433, 517
BsaAI YACGTR 3 cut(s) 502, 675, 1412
BsaBI GATNNNNATC 1 cut(s) 773
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 372
BsaWI WCCGGW 2 cut(s) 1016, 1321
BsaXI ACNNNNNCTCC 4 cut(s) 890, 920, 1354, 1384
Bsc4I CCNNNNNNNGG 5 cut(s) 75, 277, 387, 471, 1321
Bse118I RCCGGY 4 cut(s) 380, 589, 1300, 1321
Bse3DI GCAATG 1 cut(s) 1037
Bse8I GATNNNNATC 1 cut(s) 773
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 2 cut(s) 412, 857
BseJI GATNNNNATC 1 cut(s) 773
BseLI CCNNNNNNNGG 5 cut(s) 75, 277, 387, 471, 1321
BseMI GCAATG 1 cut(s) 1037
BseMII CTCAG 2 cut(s) 998, 1036
BseSI GKGCMC 1 cut(s) 422
BseXI GCAGC 1 cut(s) 396
BseYI CCCAGC 4 cut(s) 189, 531, 818, 861
BsgI GTGCAG 1 cut(s) 557
Bsh1236I CGCG 6 cut(s) 294, 903, 1155, 1160, 1217, 1358
Bsh1285I CGRYCG 1 cut(s) 1326
BshFI GGCC 6 cut(s) 5, 420, 497, 786, 1201, 1304
BshTI ACCGGT 1 cut(s) 1321
BsiEI CGRYCG 1 cut(s) 1326
BsiHKAI GWGCWC 4 cut(s) 83, 111, 148, 510
BsiHKCI CYCGRG 3 cut(s) 287, 465, 1052
BsiWI CGTACG 1 cut(s) 693
BslFI GGGAC 6 cut(s) 172, 253, 448, 933, 1351, 1420
BslI CCNNNNNNNGG 5 cut(s) 75, 277, 387, 471, 1321
BsmAI GTCTC 2 cut(s) 683, 728
BsmFI GGGAC 6 cut(s) 172, 253, 448, 933, 1351, 1420
BsmI GAATGC 1 cut(s) 27
BsnI GGCC 6 cut(s) 5, 420, 497, 786, 1201, 1304
BsoBI CYCGRG 3 cut(s) 287, 465, 1052
Bsp120I GGGCCC 1 cut(s) 418
Bsp1286I GDGCHC 5 cut(s) 83, 111, 148, 422, 510
Bsp143I GATC 4 cut(s) 311, 331, 474, 1423
Bsp68I TCGCGA 1 cut(s) 1155
BspANI GGCC 6 cut(s) 5, 420, 497, 786, 1201, 1304
BspCNI CTCAG 2 cut(s) 997, 1035
BspFNI CGCG 6 cut(s) 294, 903, 1155, 1160, 1217, 1358
BspHI TCATGA 1 cut(s) 1164
BspLI GGNNCC 3 cut(s) 420, 421, 1254
BspMAI CTGCAG 1 cut(s) 1131
BspPI GGATC 2 cut(s) 339, 482
BspQI GCTCTTC 1 cut(s) 1235
BsrBI CCGCTC 4 cut(s) 616, 815, 943, 1080
BsrDI GCAATG 1 cut(s) 1037
BsrFI RCCGGY 4 cut(s) 380, 589, 1300, 1321
BssAI RCCGGY 4 cut(s) 380, 589, 1300, 1321
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 4 cut(s) 311, 331, 474, 1423
BssNI GRCGYC 1 cut(s) 114
BssSI CACGAG 2 cut(s) 619, 893
Bst2BI CACGAG 2 cut(s) 619, 893
Bst4CI ACNGT 7 cut(s) 53, 106, 219, 698, 715, 1261, 1296
Bst6I CTCTTC 1 cut(s) 1235
BstACI GRCGYC 1 cut(s) 114
BstBAI YACGTR 3 cut(s) 502, 675, 1412
BstC8I GCNNGC 5 cut(s) 83, 206, 570, 591, 1203
BstDEI CTNAG 4 cut(s) 140, 984, 1022, 1176
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 2 cut(s) 412, 857
BstFNI CGCG 6 cut(s) 294, 903, 1155, 1160, 1217, 1358
BstHHI GCGC 4 cut(s) 294, 545, 554, 1360
BstKTI GATC 4 cut(s) 314, 334, 477, 1426
BstMAI GTCTC 2 cut(s) 683, 728
BstMBI GATC 4 cut(s) 311, 331, 474, 1423
BstMCI CGRYCG 1 cut(s) 1326
BstMWI GCNNNNNNNGC 7 cut(s) 549, 551, 558, 962, 1032, 1086, 1372
BstPAI GACNNNNGTC 1 cut(s) 1412
BstSCI CCNGG 3 cut(s) 74, 431, 515
BstSFI CTRYAG 1 cut(s) 1127
BstSLI GKGCMC 1 cut(s) 422
BstUI CGCG 6 cut(s) 294, 903, 1155, 1160, 1217, 1358
BstV1I GCAGC 1 cut(s) 396
BstX2I RGATCY 2 cut(s) 311, 331
BstXI CCANNNNNNTGG 1 cut(s) 328
BstYI RGATCY 2 cut(s) 311, 331
BsuI GTATCC 1 cut(s) 154
BsuRI GGCC 6 cut(s) 5, 420, 497, 786, 1201, 1304
BtgI CCRYGG 1 cut(s) 372
BtrI CACGTC 2 cut(s) 357, 876
BtsCI GGATG 2 cut(s) 412, 857
BtsI GCAGTG 1 cut(s) 545
BtsIMutI CAGTG 3 cut(s) 49, 545, 711
BtuMI TCGCGA 1 cut(s) 1155
Cac8I GCNNGC 5 cut(s) 83, 206, 570, 591, 1203
CciI TCATGA 1 cut(s) 1164
CfoI GCGC 4 cut(s) 294, 545, 554, 1360
Cfr10I RCCGGY 4 cut(s) 380, 589, 1300, 1321
Cfr13I GGNCC 6 cut(s) 301, 361, 418, 419, 898, 1199
CpoI CGGWCCG 1 cut(s) 301
CseI GACGC 4 cut(s) 145, 1015, 1166, 1223
Csp6I GTAC 4 cut(s) 215, 457, 694, 709
CspAI ACCGGT 1 cut(s) 1321
CspI CGGWCCG 1 cut(s) 301
CviAII CATG 1 cut(s) 1165
CviQI GTAC 4 cut(s) 215, 457, 694, 709
DdeI CTNAG 4 cut(s) 140, 984, 1022, 1176
DpnI GATC 4 cut(s) 313, 333, 476, 1425
DpnII GATC 4 cut(s) 311, 331, 474, 1423
DriI GACNNNNNGTC 2 cut(s) 265, 1025
EaeI YGGCCR 3 cut(s) 3, 784, 1302
Eam1104I CTCTTC 1 cut(s) 1235
Eam1105I GACNNNNNGTC 2 cut(s) 265, 1025
EarI CTCTTC 1 cut(s) 1235
EciI GGCGGA 1 cut(s) 1104
Ecl136II GAGCTC 2 cut(s) 81, 146
Eco147I AGGCCT 1 cut(s) 497
Eco24I GRGCYC 3 cut(s) 83, 148, 422
Eco47I GGWCC 3 cut(s) 301, 361, 898
Eco53kI GAGCTC 2 cut(s) 81, 146
Eco72I CACGTG 2 cut(s) 675, 1412
Eco88I CYCGRG 3 cut(s) 287, 465, 1052
EcoICRI GAGCTC 2 cut(s) 81, 146
EcoO109I RGGNCCY 2 cut(s) 418, 419
EcoRI GAATTC 1 cut(s) 1432
EcoT38I GRGCYC 3 cut(s) 83, 148, 422
FaeI CATG 1 cut(s) 1168
FaiI YATR 4 cut(s) 201, 746, 1141, 1166
FaqI GGGAC 6 cut(s) 172, 253, 448, 933, 1351, 1420
FatI CATG 1 cut(s) 1164
FauI CCCGC 2 cut(s) 380, 743
FblI GTMKAC 2 cut(s) 89, 1326
Fnu4HI GCNGC 9 cut(s) 120, 385, 451, 614, 816, 838, 841, 941, 976
FokI GGATG 2 cut(s) 419, 844
FriOI GRGCYC 3 cut(s) 83, 148, 422
Fsp4HI GCNGC 9 cut(s) 120, 385, 451, 614, 816, 838, 841, 941, 976
FspAI RTGCGCAY 1 cut(s) 544
FspBI CTAG 1 cut(s) 1427
FspI TGCGCA 1 cut(s) 544
GlaI GCGC 4 cut(s) 293, 544, 553, 1359
GluI GCNGC 9 cut(s) 120, 385, 451, 614, 816, 838, 841, 941, 976
GsaI CCCAGC 4 cut(s) 193, 535, 822, 865
HaeIII GGCC 6 cut(s) 5, 420, 497, 786, 1201, 1304
HgaI GACGC 4 cut(s) 145, 1015, 1166, 1223
HhaI GCGC 4 cut(s) 294, 545, 554, 1360
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 1 cut(s) 1168
Hin6I GCGC 4 cut(s) 292, 543, 552, 1358
HinP1I GCGC 4 cut(s) 292, 543, 552, 1358
HincII GTYRAC 2 cut(s) 90, 1327
HindII GTYRAC 2 cut(s) 90, 1327
HphI GGTGA 3 cut(s) 310, 710, 1129
Hpy166II GTNNAC 5 cut(s) 90, 711, 898, 1191, 1327
Hpy188III TCNNGA 9 cut(s) 228, 465, 472, 1045, 1052, 1066, 1154, 1165, 1315
Hpy8I GTNNAC 5 cut(s) 90, 711, 898, 1191, 1327
HpyAV CCTTC 3 cut(s) 258, 616, 757
HpyCH4III ACNGT 7 cut(s) 53, 106, 219, 698, 715, 1261, 1296
HpyCH4IV ACGT 9 cut(s) 114, 356, 501, 674, 875, 995, 1146, 1340, 1411
HpyCH4V TGCA 4 cut(s) 27, 538, 639, 1129
HpyF10VI GCNNNNNNNGC 7 cut(s) 549, 551, 558, 962, 1032, 1086, 1372
HpyF3I CTNAG 4 cut(s) 140, 984, 1022, 1176
HpySE526I ACGT 9 cut(s) 114, 356, 501, 674, 875, 995, 1146, 1340, 1411
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 1 cut(s) 1168
HspAI GCGC 4 cut(s) 292, 543, 552, 1358
KroI GCCGGC 1 cut(s) 589
KroNI GCCGGC 1 cut(s) 591
Kzo9I GATC 4 cut(s) 311, 331, 474, 1423
LguI GCTCTTC 1 cut(s) 1235
LmnI GCTCC 6 cut(s) 78, 812, 948, 970, 1047, 1077
Lsp1109I GCAGC 1 cut(s) 396
LweI GCATC 2 cut(s) 391, 926
MaeI CTAG 1 cut(s) 1427
MaeII ACGT 9 cut(s) 114, 356, 501, 674, 875, 995, 1146, 1340, 1411
MalI GATC 4 cut(s) 313, 333, 476, 1425
MbiI CCGCTC 4 cut(s) 616, 815, 943, 1080
MboI GATC 4 cut(s) 311, 331, 474, 1423
MboII GAAGA 2 cut(s) 574, 1252
MflI RGATCY 2 cut(s) 311, 331
MhlI GDGCHC 5 cut(s) 83, 111, 148, 422, 510
MlsI TGGCCA 1 cut(s) 786
MluCI AATT 3 cut(s) 231, 1309, 1432
MluI ACGCGT 2 cut(s) 1158, 1215
MluNI TGGCCA 1 cut(s) 786
MmeI TCCRAC 8 cut(s) 135, 309, 339, 644, 790, 933, 1280, 1420
Mox20I TGGCCA 1 cut(s) 786
MroNI GCCGGC 1 cut(s) 589
MscI TGGCCA 1 cut(s) 786
MseI TTAA 1 cut(s) 366
MslI CAYNNNNRTG 1 cut(s) 1115
Msp20I TGGCCA 1 cut(s) 786
MspA1I CMGCKG 4 cut(s) 387, 531, 988, 1375
MspR9I CCNGG 3 cut(s) 76, 433, 517
Mva1269I GAATGC 1 cut(s) 27
MvnI CGCG 6 cut(s) 294, 903, 1155, 1160, 1217, 1358
MwoI GCNNNNNNNGC 7 cut(s) 549, 551, 558, 962, 1032, 1086, 1372
NaeI GCCGGC 1 cut(s) 591
NciI CCSGG 3 cut(s) 76, 433, 517
NdeII GATC 4 cut(s) 311, 331, 474, 1423
NgoMIV GCCGGC 1 cut(s) 589
NlaIII CATG 1 cut(s) 1168
NlaIV GGNNCC 3 cut(s) 420, 421, 1254
NmeAIII GCCGAG 3 cut(s) 513, 813, 893
NmuCI GTSAC 5 cut(s) 521, 698, 727, 876, 1117
NruI TCGCGA 1 cut(s) 1155
NsbI TGCGCA 1 cut(s) 544
OliI CACNNNNGTG 1 cut(s) 1115
PaeR7I CTCGAG 1 cut(s) 287
PagI TCATGA 1 cut(s) 1164
PceI AGGCCT 1 cut(s) 497
PciSI GCTCTTC 1 cut(s) 1235
PcsI WCGNNNNNNNCGW 7 cut(s) 131, 507, 617, 900, 1152, 1221, 1337
PctI GAATGC 1 cut(s) 27
PdiI GCCGGC 1 cut(s) 591
PfeI GAWTC 4 cut(s) 127, 626, 1048, 1092
Pfl23II CGTACG 1 cut(s) 693
PflFI GACNNNGTC 2 cut(s) 299, 1261
PfoI TCCNGGA 2 cut(s) 74, 431
PinAI ACCGGT 1 cut(s) 1321
PkrI GCNGC 9 cut(s) 121, 386, 452, 615, 817, 839, 842, 942, 977
PmaCI CACGTG 2 cut(s) 675, 1412
PmlI CACGTG 2 cut(s) 675, 1412
Ppu21I YACGTR 3 cut(s) 502, 675, 1412
PshAI GACNNNNGTC 1 cut(s) 1412
Psp124BI GAGCTC 2 cut(s) 83, 148
PspCI CACGTG 2 cut(s) 675, 1412
PspFI CCCAGC 4 cut(s) 189, 531, 818, 861
PspLI CGTACG 1 cut(s) 693
PspN4I GGNNCC 3 cut(s) 420, 421, 1254
PspOMI GGGCCC 1 cut(s) 418
PspPI GGNCC 6 cut(s) 301, 361, 418, 419, 898, 1199
PspXI VCTCGAGB 1 cut(s) 287
PstI CTGCAG 1 cut(s) 1131
PsuI RGATCY 2 cut(s) 311, 331
PsyI GACNNNGTC 2 cut(s) 299, 1261
PvuII CAGCTG 2 cut(s) 531, 988
RruI TCGCGA 1 cut(s) 1155
RsaI GTAC 4 cut(s) 216, 458, 695, 710
RsaNI GTAC 4 cut(s) 215, 457, 694, 709
RseI CAYNNNNRTG 1 cut(s) 1115
Rsr2I CGGWCCG 1 cut(s) 301
RsrII CGGWCCG 1 cut(s) 301
SacI GAGCTC 2 cut(s) 83, 148
SalI GTCGAC 2 cut(s) 88, 1325
SapI GCTCTTC 1 cut(s) 1235
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 9 cut(s) 120, 385, 451, 614, 816, 838, 841, 941, 976
Sau3AI GATC 4 cut(s) 311, 331, 474, 1423
Sau96I GGNCC 6 cut(s) 301, 361, 418, 419, 898, 1199
ScaI AGTACT 1 cut(s) 458
ScrFI CCNGG 3 cut(s) 76, 433, 517
SduI GDGCHC 5 cut(s) 83, 111, 148, 422, 510
SfaNI GCATC 2 cut(s) 391, 926
SfcI CTRYAG 1 cut(s) 1127
Sfr274I CTCGAG 1 cut(s) 287
SinI GGWCC 3 cut(s) 301, 361, 898
SlaI CTCGAG 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 1115
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 3 cut(s) 231, 1309, 1432
SseBI AGGCCT 1 cut(s) 497
SspMI CTAG 1 cut(s) 1427
SstI GAGCTC 2 cut(s) 83, 148
StuI AGGCCT 1 cut(s) 497
StyD4I CCNGG 3 cut(s) 74, 431, 515
TaaI ACNGT 7 cut(s) 53, 106, 219, 698, 715, 1261, 1296
TaiI ACGT 9 cut(s) 117, 359, 504, 677, 878, 998, 1149, 1343, 1414
TaqII GACCGA 2 cut(s) 289, 998
TasI AATT 3 cut(s) 231, 1309, 1432
TatI WGTACW 1 cut(s) 456
TauI GCSGC 8 cut(s) 122, 453, 616, 818, 840, 843, 943, 978
TfiI GAWTC 4 cut(s) 127, 626, 1048, 1092
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 3 cut(s) 56, 545, 718
TseFI GTSAC 5 cut(s) 521, 698, 727, 876, 1117
TseI GCWGC 1 cut(s) 384
Tsp45I GTSAC 5 cut(s) 521, 698, 727, 876, 1117
TspDTI ATGAA 2 cut(s) 1153, 1181
TspGWI ACGGA 4 cut(s) 746, 935, 957, 1148
TspRI CASTG 3 cut(s) 56, 545, 718
Tth111I GACNNNGTC 2 cut(s) 299, 1261
VpaK11BI GGWCC 3 cut(s) 301, 361, 898
XapI RAATTY 3 cut(s) 231, 1309, 1432
XhoI CTCGAG 1 cut(s) 287
XmiI GTMKAC 2 cut(s) 89, 1326
XspI CTAG 1 cut(s) 1427
ZraI GACGTC 1 cut(s) 115
ZrmI AGTACT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.