Rh4BG350900

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014902
Physical Location & Seq
Reverse (-)
51203957 .. 51205435
1479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG350900.1

Sequence Viewer

Length: 1479 bp
ATGGCCCAGATGGGTTTTCTCTGCCACTACATTCTCTTTGCTTTCCTTTCTTACTCTGCTCACTCCCGCAACTTACCGGCCACCACCACCACCTCCAAAACGACGGTGCTCGACGTCGCCGCTTCCATTCAGGAGGCCCTAAACGCCATGTCGTCCGAGCACTACTCTTACAGTGGAGCCACTCAGTCATTGAGCCAAGAACACCAAACCTTCTCAGTTTCATCTTCACTCTCTCTTCAACTACATTCCCGAATTTCACTCCACCAGCATTCTCACAAAGACTACAAGTCGCTCAGTTTGGCTCGACTCGGGCGAGACTCCGCCCGAGTCAGATCTCTAACCACCAGGTTAGATCTGGCGGTGAGAGGCATTGCCACGTCGGATCTCAAACCCGTGGATACCCAACATTTCGGTACCGACGGGTTCGAGAGCCCCGTAGTTTCGGGGACGAGTGAGGGCAGCGGCGAGTACTTCTCCCGAGTCGGAATCGGCAAACCGCCCAGCCAGGTCTACGTCGTGCTCGACACGGGCAGTGACGTCAGCTGGGTCCAATGCGCGCCGTGCGCCGACTGTTACCAGCAAGCCGACCCCATTTTCGAGCCGGATTCCTCGGCTTCGTTCTCGCCGCTGTCGTGCGAGACCCGGCAGTGCAAGTCCCTCGACGTGTTCGAGTGCCGCAACAACACGTGTCTCTACGAGGTGGCCTACGGCGACGGCTCCTACACCGTCGGCGACTTCGTGACGGAGACGATCAGCATCGGCGGCGAGTCGGTGAAAAACGTTGCCATTGGATGTGGGCATAACAACGAAGGACTGTTTGTCGGTGCGGCTGGCTTGCTCGGGCTCGGCGGGGGGCCGTTGTCGTTTCCTTCCCAGCTCAACGCCACGTCGTTTTCGTACTGCCTCGTCGACCGTGACTCTGACTCGGCCTCGACTCTGGAATTCAACTCGGCAATCCCTCGCAACGCCGTCACGGCCCCGTTACGGCGCAACCCCCAGCTCGACACTTTCTACTACGTCGGACTGACCGGAATCAGCGTCGGCGGCGATATGCTTAAGATTCCCGACACGGCGTTCCTGATAGACGAGAGCGGAAACGGCGGAATCATCATCGACTCCGGCACGGCGGTGACCAGGCTCCAGACGGACACCTACGACGCGCTCCGCAGCGCGTTCGTGAACGGCACGAAGGGGCTGCAGTCCTCGAGCGGCGTGGCGCTGTTCGACGCGTGCTACGACCTGTCGTCGAATAAGACCGTGGAAGTACCAACGCTGTCGTTTCACTTCGAGGACGGGAAAGTGTTACCGTTGCCGGCGAAGAATTACCTGATTCCGGTGGACTCGCAGGGGACGTTCTGCTTTGCTTTCGCGCCGACGAGCTCGGCGCTGTCGATAATCGGAAACGTGCAGCAGCAAGGGACACGTGTCAGTTTCGACATAGCTAATTCGGTGGTTGGATTCTCTCCCAACCAATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

492

Amino Acids

52.42

Weight (kDa)

4.98

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 156 - 489 4.6e-21 Eukaryotic aspartyl protease
TAXi_N PF14543 157 - 315 8.9e-49 Xylanase inhibitor N-terminal
TAXi_C PF14541 338 - 488 8.4e-38 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014902)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25510
fragaria_vesca FvH4_4g27770
malus_domestica MD13G1044900.v1.1
prunus_persica Prupe.1G307100_v2.0.a1
pyrus_communis pycom13g03970 pycom16g04080
rosa_chinensis RchiOBHm_Chr4g0435631
rosa_laevigata RLG00000006575
rosa_multiflora Rmu_sc0000051.1_g000004
rosa_roxburghii Rroxscaffold_5G00376850
rosa_rugosa Rorug04G0287400
rosa_samantha Rh4AG342600 Rh4BG350900 Rh4CG365600 Rh4DG345300
rosa_wichuraiana Rw4G029900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 117, 540
Acc65I GGTACC 1 cut(s) 413
AccB1I GGYRCC 1 cut(s) 413
AccBSI CCGCTC 2 cut(s) 1092, 1209
AccI GTMKAC 2 cut(s) 510, 909
AccII CGCG 5 cut(s) 557, 1160, 1172, 1229, 1370
AclI AACGTT 1 cut(s) 780
AclWI GGATC 1 cut(s) 390
AcoI YGGCCR 1 cut(s) 78
AcsI RAATTY 2 cut(s) 252, 941
AcvI CACGTG 2 cut(s) 687, 1424
AcyI GRCGYC 2 cut(s) 114, 537
AfaI GTAC 4 cut(s) 415, 470, 899, 1266
AfiI CCNNNNNNNGG 2 cut(s) 984, 1333
AflII CTTAAG 1 cut(s) 1055
AflIII ACRYGT 6 cut(s) 663, 684, 686, 1227, 1421, 1423
AgsI TTSAA 2 cut(s) 239, 946
AhdI GACNNNNNGTC 3 cut(s) 286, 818, 1243
AjiI CACGTC 3 cut(s) 378, 664, 888
AjnI CCWGG 3 cut(s) 344, 504, 1133
AleI CACNNNNGTG 1 cut(s) 1127
AluBI AGCT 5 cut(s) 543, 877, 1000, 1380, 1442
AluI AGCT 5 cut(s) 543, 877, 1000, 1380, 1442
Alw21I GWGCWC 4 cut(s) 111, 162, 522, 1382
Alw26I GTCTC 4 cut(s) 309, 632, 695, 740
AlwI GGATC 1 cut(s) 390
Ama87I CYCGRG 5 cut(s) 308, 324, 477, 839, 1204
AoxI GGCC 7 cut(s) 3, 78, 135, 702, 854, 927, 975
ApeKI GCWGC 5 cut(s) 459, 1167, 1195, 1408, 1411
ApoI RAATTY 2 cut(s) 252, 941
ArsI GACNNNNNNTTYG 2 cut(s) 578, 610
Asp718I GGTACC 1 cut(s) 413
AspLEI GCGC 9 cut(s) 557, 559, 566, 990, 1162, 1172, 1219, 1372, 1387
AspS9I GGNCC 5 cut(s) 4, 136, 547, 854, 976
AsuC2I CCSGG 1 cut(s) 643
AsuHPI GGTGA 3 cut(s) 373, 784, 1141
AvaI CYCGRG 5 cut(s) 308, 324, 477, 839, 1204
AvaII GGWCC 1 cut(s) 547
BanI GGYRCC 1 cut(s) 413
BanII GRGCYC 3 cut(s) 434, 846, 1382
BbrPI CACGTG 2 cut(s) 687, 1424
Bbv12I GWGCWC 4 cut(s) 111, 162, 522, 1382
BbvI GCAGC 5 cut(s) 471, 1179, 1182, 1420, 1423
BccI CCATC 1 cut(s) 4
BcgI CGANNNNNNTGC 6 cut(s) 640, 674, 1156, 1177, 1190, 1211
BciT130I CCWGG 3 cut(s) 346, 506, 1135
BciVI GTATCC 1 cut(s) 391
BcnI CCSGG 1 cut(s) 643
BcoDI GTCTC 4 cut(s) 309, 632, 695, 740
BfaI CTAG 1 cut(s) 1477
BfmI CTRYAG 1 cut(s) 1196
BfoI RGCGCY 2 cut(s) 1220, 1388
BfrI CTTAAG 1 cut(s) 1055
BfuI GTATCC 1 cut(s) 391
BglI GCCNNNNNGGC 1 cut(s) 974
BglII AGATCT 2 cut(s) 332, 352
BmcAI AGTACT 1 cut(s) 470
Bme1390I CCNGG 4 cut(s) 346, 506, 643, 1135
Bme18I GGWCC 1 cut(s) 547
BmeRI GACNNNNNGTC 3 cut(s) 286, 818, 1243
BmeT110I CYCGRG 5 cut(s) 308, 324, 477, 839, 1204
BmgBI CACGTC 3 cut(s) 378, 664, 888
BmgT120I GGNCC 5 cut(s) 4, 136, 547, 854, 976
BmiI GGNNCC 7 cut(s) 178, 415, 548, 718, 855, 978, 1139
BmrFI CCNGG 4 cut(s) 346, 506, 643, 1135
BmsI GCATC 1 cut(s) 765
BoxI GACNNNNGTC 1 cut(s) 1424
BplI GAGNNNNNCTC 4 cut(s) 149, 181, 458, 490
BpmI CTGGAG 1 cut(s) 1124
BpuMI CCSGG 1 cut(s) 643
BsaAI YACGTR 2 cut(s) 687, 1424
BsaBI GATNNNNATC 1 cut(s) 755
BsaHI GRCGYC 2 cut(s) 114, 537
BsaI GGTCTC 1 cut(s) 632
BsaJI CCNNGG 3 cut(s) 393, 609, 1257
BsaWI WCCGGW 2 cut(s) 1028, 1333
Bsc4I CCNNNNNNNGG 2 cut(s) 984, 1333
Bse118I RCCGGY 2 cut(s) 76, 1312
Bse3DI GCAATG 1 cut(s) 369
Bse8I GATNNNNATC 1 cut(s) 755
BseBI CCWGG 3 cut(s) 346, 506, 1135
BseDI CCNNGG 3 cut(s) 393, 609, 1257
BseGI GGATG 1 cut(s) 797
BseJI GATNNNNATC 1 cut(s) 755
BseLI CCNNNNNNNGG 2 cut(s) 984, 1333
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 3 cut(s) 197, 228, 307
BsePI GCGCGC 1 cut(s) 555
BseXI GCAGC 5 cut(s) 471, 1179, 1182, 1420, 1423
BseYI CCCAGC 4 cut(s) 500, 543, 873, 996
BsgI GTGCAG 1 cut(s) 1427
Bsh1236I CGCG 5 cut(s) 557, 1160, 1172, 1229, 1370
Bsh1285I CGRYCG 1 cut(s) 913
BshFI GGCC 7 cut(s) 5, 80, 137, 704, 856, 929, 977
BshNI GGYRCC 1 cut(s) 413
BsiEI CGRYCG 1 cut(s) 913
BsiHKAI GWGCWC 4 cut(s) 111, 162, 522, 1382
BsiHKCI CYCGRG 5 cut(s) 308, 324, 477, 839, 1204
BsiSI CCGG 7 cut(s) 77, 602, 643, 1029, 1119, 1313, 1334
BslFI GGGAC 4 cut(s) 460, 640, 1363, 1432
BslI CCNNNNNNNGG 2 cut(s) 984, 1333
BsmAI GTCTC 4 cut(s) 309, 632, 695, 740
BsmBI CGTCTC 1 cut(s) 740
BsmFI GGGAC 4 cut(s) 460, 640, 1363, 1432
BsmI GAATGC 1 cut(s) 268
BsnI GGCC 7 cut(s) 5, 80, 137, 704, 856, 929, 977
Bso31I GGTCTC 1 cut(s) 632
BsoBI CYCGRG 5 cut(s) 308, 324, 477, 839, 1204
Bsp1286I GDGCHC 6 cut(s) 111, 162, 434, 522, 846, 1382
Bsp143I GATC 4 cut(s) 332, 352, 382, 750
BspANI GGCC 7 cut(s) 5, 80, 137, 704, 856, 929, 977
BspCNI CTCAG 3 cut(s) 196, 227, 306
BspFNI CGCG 5 cut(s) 557, 1160, 1172, 1229, 1370
BspLI GGNNCC 7 cut(s) 178, 415, 548, 718, 855, 978, 1139
BspMAI CTGCAG 1 cut(s) 1200
BspPI GGATC 1 cut(s) 390
BspT107I GGYRCC 1 cut(s) 413
BspTI CTTAAG 1 cut(s) 1055
BspTNI GGTCTC 1 cut(s) 632
BsrBI CCGCTC 2 cut(s) 1092, 1209
BsrDI GCAATG 1 cut(s) 369
BsrFI RCCGGY 2 cut(s) 76, 1312
BssAI RCCGGY 2 cut(s) 76, 1312
BssECI CCNNGG 3 cut(s) 393, 609, 1257
BssHII GCGCGC 1 cut(s) 555
BssMI GATC 4 cut(s) 332, 352, 382, 750
BssNI GRCGYC 2 cut(s) 114, 537
Bst2UI CCWGG 3 cut(s) 346, 506, 1135
Bst4CI ACNGT 8 cut(s) 106, 173, 572, 727, 816, 914, 1258, 1308
Bst6I CTCTTC 1 cut(s) 240
BstACI GRCGYC 2 cut(s) 114, 537
BstAFI CTTAAG 1 cut(s) 1055
BstBAI YACGTR 2 cut(s) 687, 1424
BstC8I GCNNGC 6 cut(s) 557, 582, 832, 836, 1231, 1314
BstDEI CTNAG 3 cut(s) 183, 214, 293
BstDSI CCRYGG 2 cut(s) 393, 1257
BstEII GGTNACC 1 cut(s) 1129
BstF5I GGATG 1 cut(s) 797
BstFNI CGCG 5 cut(s) 557, 1160, 1172, 1229, 1370
BstH2I RGCGCY 2 cut(s) 1220, 1388
BstHHI GCGC 9 cut(s) 557, 559, 566, 990, 1162, 1172, 1219, 1372, 1387
BstKTI GATC 4 cut(s) 335, 355, 385, 753
BstMAI GTCTC 4 cut(s) 309, 632, 695, 740
BstMBI GATC 4 cut(s) 332, 352, 382, 750
BstMCI CGRYCG 1 cut(s) 913
BstMWI GCNNNNNNNGC 7 cut(s) 143, 561, 563, 762, 974, 1044, 1098
BstNI CCWGG 3 cut(s) 346, 506, 1135
BstPAI GACNNNNGTC 1 cut(s) 1424
BstPI GGTNACC 1 cut(s) 1129
BstSCI CCNGG 4 cut(s) 344, 504, 641, 1133
BstSFI CTRYAG 1 cut(s) 1196
BstUI CGCG 5 cut(s) 557, 1160, 1172, 1229, 1370
BstV1I GCAGC 5 cut(s) 471, 1179, 1182, 1420, 1423
BstX2I RGATCY 3 cut(s) 332, 352, 382
BstYI RGATCY 3 cut(s) 332, 352, 382
BsuI GTATCC 1 cut(s) 391
BsuRI GGCC 7 cut(s) 5, 80, 137, 704, 856, 929, 977
BtgI CCRYGG 2 cut(s) 393, 1257
BtrI CACGTC 3 cut(s) 378, 664, 888
BtsCI GGATG 1 cut(s) 797
BtsI GCAGTG 2 cut(s) 538, 653
BtsIMutI CAGTG 3 cut(s) 178, 538, 653
Cac8I GCNNGC 6 cut(s) 557, 582, 832, 836, 1231, 1314
CfoI GCGC 9 cut(s) 557, 559, 566, 990, 1162, 1172, 1219, 1372, 1387
Cfr10I RCCGGY 2 cut(s) 76, 1312
Cfr13I GGNCC 5 cut(s) 4, 136, 547, 854, 976
CseI GACGC 3 cut(s) 1027, 1166, 1235
CsiI ACCWGGT 1 cut(s) 344
Csp6I GTAC 4 cut(s) 414, 469, 898, 1265
CviAII CATG 1 cut(s) 148
CviQI GTAC 4 cut(s) 414, 469, 898, 1265
DdeI CTNAG 3 cut(s) 183, 214, 293
DpnI GATC 4 cut(s) 334, 354, 384, 752
DpnII GATC 4 cut(s) 332, 352, 382, 750
DriI GACNNNNNGTC 3 cut(s) 286, 818, 1243
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 1 cut(s) 240
Eam1105I GACNNNNNGTC 3 cut(s) 286, 818, 1243
EarI CTCTTC 1 cut(s) 240
EciI GGCGGA 2 cut(s) 310, 1116
Ecl136II GAGCTC 1 cut(s) 1380
Eco24I GRGCYC 3 cut(s) 434, 846, 1382
Eco31I GGTCTC 1 cut(s) 632
Eco47I GGWCC 1 cut(s) 547
Eco53kI GAGCTC 1 cut(s) 1380
Eco72I CACGTG 2 cut(s) 687, 1424
Eco88I CYCGRG 5 cut(s) 308, 324, 477, 839, 1204
Eco91I GGTNACC 1 cut(s) 1129
EcoICRI GAGCTC 1 cut(s) 1380
EcoO109I RGGNCCY 1 cut(s) 136
EcoO65I GGTNACC 1 cut(s) 1129
EcoRI GAATTC 1 cut(s) 941
EcoRII CCWGG 3 cut(s) 344, 504, 1133
EcoT38I GRGCYC 3 cut(s) 434, 846, 1382
Esp3I CGTCTC 1 cut(s) 740
FaeI CATG 1 cut(s) 151
FaiI YATR 4 cut(s) 149, 801, 1052, 1439
FaqI GGGAC 4 cut(s) 460, 640, 1363, 1432
FatI CATG 1 cut(s) 147
FauI CCCGC 2 cut(s) 74, 842
FblI GTMKAC 2 cut(s) 510, 909
FokI GGATG 1 cut(s) 804
FriOI GRGCYC 3 cut(s) 434, 846, 1382
FspBI CTAG 1 cut(s) 1477
GlaI GCGC 9 cut(s) 556, 558, 565, 989, 1161, 1171, 1218, 1371, 1386
GsaI CCCAGC 4 cut(s) 504, 547, 877, 1000
GsuI CTGGAG 1 cut(s) 1124
HaeII RGCGCY 2 cut(s) 1220, 1388
HaeIII GGCC 7 cut(s) 5, 80, 137, 704, 856, 929, 977
HapII CCGG 7 cut(s) 77, 602, 643, 1029, 1119, 1313, 1334
HgaI GACGC 3 cut(s) 1027, 1166, 1235
HhaI GCGC 9 cut(s) 557, 559, 566, 990, 1162, 1172, 1219, 1372, 1387
Hin1I GRCGYC 2 cut(s) 114, 537
Hin1II CATG 1 cut(s) 151
Hin6I GCGC 9 cut(s) 555, 557, 564, 988, 1160, 1170, 1217, 1370, 1385
HinP1I GCGC 9 cut(s) 555, 557, 564, 988, 1160, 1170, 1217, 1370, 1385
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HpaII CCGG 7 cut(s) 77, 602, 643, 1029, 1119, 1313, 1334
HphI GGTGA 3 cut(s) 373, 784, 1141
Hpy166II GTNNAC 4 cut(s) 511, 910, 1180, 1339
Hpy188I TCNGA 7 cut(s) 157, 332, 382, 485, 922, 1022, 1400
Hpy8I GTNNAC 4 cut(s) 511, 910, 1180, 1339
HpyAV CCTTC 4 cut(s) 220, 803, 879, 1183
HpyCH4III ACNGT 8 cut(s) 106, 173, 572, 727, 816, 914, 1258, 1308
HpyCH4V TGCA 3 cut(s) 651, 1198, 1408
HpyF10VI GCNNNNNNNGC 7 cut(s) 143, 561, 563, 762, 974, 1044, 1098
HpyF3I CTNAG 3 cut(s) 183, 214, 293
Hsp92I GRCGYC 2 cut(s) 114, 537
Hsp92II CATG 1 cut(s) 151
HspAI GCGC 9 cut(s) 555, 557, 564, 988, 1160, 1170, 1217, 1370, 1385
KpnI GGTACC 1 cut(s) 417
KroI GCCGGC 1 cut(s) 1312
KroNI GCCGGC 1 cut(s) 1314
Kzo9I GATC 4 cut(s) 332, 352, 382, 750
LmnI GCTCC 4 cut(s) 176, 722, 1143, 1167
Lsp1109I GCAGC 5 cut(s) 471, 1179, 1182, 1420, 1423
LweI GCATC 1 cut(s) 765
MabI ACCWGGT 1 cut(s) 344
MaeI CTAG 1 cut(s) 1477
MaeIII GTNAC 8 cut(s) 533, 572, 739, 914, 970, 981, 1129, 1302
MalI GATC 4 cut(s) 334, 354, 384, 752
MbiI CCGCTC 2 cut(s) 1092, 1209
MboI GATC 4 cut(s) 332, 352, 382, 750
MboII GAAGA 3 cut(s) 216, 227, 1330
MflI RGATCY 3 cut(s) 332, 352, 382
MhlI GDGCHC 6 cut(s) 111, 162, 434, 522, 846, 1382
MluCI AATT 4 cut(s) 252, 941, 1321, 1444
MluI ACGCGT 1 cut(s) 1227
MmeI TCCRAC 4 cut(s) 360, 463, 1000, 1435
MroNI GCCGGC 1 cut(s) 1312
MseI TTAA 1 cut(s) 1056
MslI CAYNNNNRTG 1 cut(s) 1127
MspA1I CMGCKG 3 cut(s) 462, 543, 628
MspCI CTTAAG 1 cut(s) 1055
MspI CCGG 7 cut(s) 77, 602, 643, 1029, 1119, 1313, 1334
MspR9I CCNGG 4 cut(s) 346, 506, 643, 1135
Mva1269I GAATGC 1 cut(s) 268
MvaI CCWGG 3 cut(s) 346, 506, 1135
MvnI CGCG 5 cut(s) 557, 1160, 1172, 1229, 1370
MwoI GCNNNNNNNGC 7 cut(s) 143, 561, 563, 762, 974, 1044, 1098
NaeI GCCGGC 1 cut(s) 1314
NciI CCSGG 1 cut(s) 643
NdeII GATC 4 cut(s) 332, 352, 382, 750
NgoMIV GCCGGC 1 cut(s) 1312
NlaIII CATG 1 cut(s) 151
NlaIV GGNNCC 7 cut(s) 178, 415, 548, 718, 855, 978, 1139
NmeAIII GCCGAG 5 cut(s) 590, 825, 905, 929, 1361
NmuCI GTSAC 5 cut(s) 533, 739, 914, 970, 1129
OliI CACNNNNGTG 1 cut(s) 1127
PaeR7I CTCGAG 1 cut(s) 1204
PauI GCGCGC 1 cut(s) 555
PctI GAATGC 1 cut(s) 268
PdiI GCCGGC 1 cut(s) 1314
PfeI GAWTC 7 cut(s) 486, 605, 1032, 1060, 1104, 1330, 1458
PmaCI CACGTG 2 cut(s) 687, 1424
PmlI CACGTG 2 cut(s) 687, 1424
Ppu21I YACGTR 2 cut(s) 687, 1424
PshAI GACNNNNGTC 1 cut(s) 1424
Psp124BI GAGCTC 1 cut(s) 1382
Psp1406I AACGTT 1 cut(s) 780
Psp6I CCWGG 3 cut(s) 344, 504, 1133
PspCI CACGTG 2 cut(s) 687, 1424
PspEI GGTNACC 1 cut(s) 1129
PspFI CCCAGC 4 cut(s) 500, 543, 873, 996
PspGI CCWGG 3 cut(s) 344, 504, 1133
PspN4I GGNNCC 7 cut(s) 178, 415, 548, 718, 855, 978, 1139
PspPI GGNCC 5 cut(s) 4, 136, 547, 854, 976
PspXI VCTCGAGB 1 cut(s) 1204
PstI CTGCAG 1 cut(s) 1200
PsuI RGATCY 3 cut(s) 332, 352, 382
PteI GCGCGC 1 cut(s) 555
PvuII CAGCTG 1 cut(s) 543
RsaI GTAC 4 cut(s) 415, 470, 899, 1266
RsaNI GTAC 4 cut(s) 414, 469, 898, 1265
RseI CAYNNNNRTG 1 cut(s) 1127
SacI GAGCTC 1 cut(s) 1382
SalI GTCGAC 1 cut(s) 908
SaqAI TTAA 1 cut(s) 1056
Sau3AI GATC 4 cut(s) 332, 352, 382, 750
Sau96I GGNCC 5 cut(s) 4, 136, 547, 854, 976
ScaI AGTACT 1 cut(s) 470
ScrFI CCNGG 4 cut(s) 346, 506, 643, 1135
SduI GDGCHC 6 cut(s) 111, 162, 434, 522, 846, 1382
SexAI ACCWGGT 1 cut(s) 344
SfaNI GCATC 1 cut(s) 765
SfcI CTRYAG 1 cut(s) 1196
Sfr274I CTCGAG 1 cut(s) 1204
SinI GGWCC 1 cut(s) 547
SlaI CTCGAG 1 cut(s) 1204
SmiMI CAYNNNNRTG 1 cut(s) 1127
SmlI CTYRAG 2 cut(s) 1055, 1204
SmoI CTYRAG 2 cut(s) 1055, 1204
Sse9I AATT 4 cut(s) 252, 941, 1321, 1444
SspMI CTAG 1 cut(s) 1477
SstI GAGCTC 1 cut(s) 1382
StyD4I CCNGG 4 cut(s) 344, 504, 641, 1133
TaaI ACNGT 8 cut(s) 106, 173, 572, 727, 816, 914, 1258, 1308
TasI AATT 4 cut(s) 252, 941, 1321, 1444
TatI WGTACW 1 cut(s) 468
TauI GCSGC 8 cut(s) 122, 465, 628, 678, 765, 830, 1047, 1212
TfiI GAWTC 7 cut(s) 486, 605, 1032, 1060, 1104, 1330, 1458
Tru1I TTAA 1 cut(s) 1056
Tru9I TTAA 1 cut(s) 1056
TscAI CASTG 3 cut(s) 178, 538, 653
TseFI GTSAC 5 cut(s) 533, 739, 914, 970, 1129
TseI GCWGC 5 cut(s) 459, 1167, 1195, 1408, 1411
Tsp45I GTSAC 5 cut(s) 533, 739, 914, 970, 1129
TspDTI ATGAA 1 cut(s) 210
TspGWI ACGGA 2 cut(s) 758, 1160
TspRI CASTG 3 cut(s) 178, 538, 653
Vha464I CTTAAG 1 cut(s) 1055
VpaK11BI GGWCC 1 cut(s) 547
XapI RAATTY 2 cut(s) 252, 941
XcmI CCANNNNNNNNNTGG 1 cut(s) 352
XhoI CTCGAG 1 cut(s) 1204
XmiI GTMKAC 2 cut(s) 510, 909
XspI CTAG 1 cut(s) 1477
ZraI GACGTC 2 cut(s) 115, 538
ZrmI AGTACT 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.