FvH4_4g27770

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014902
Physical Location & Seq
Reverse (-)
28605364 .. 28606809
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27770.t1

Sequence Viewer

Length: 1446 bp
ATGGGTTCCCTCTGCTACTACATTCTCTTTGCTCTCCTCTCTTACTCTGCTCACTCCCGCAACTTACCCACCACCACCTCCAAAACCACCGTGCTCGACGTCGCCGCTTCCATTCAGGCCACCCTAAACGCCGTGTCGTCCTACTCTAACAGTGGAGCTACTCAGTCATCACTGACCCACTCAGCTTCCTCTTCCCTCTCTCTTCCACTACATTCCCGCATTTCACTCCACCAGCATTCCCACAAGGACTACAAGTCGCTCATTTCAGCTCGACTCGGGCGCGACTCGGCCCGAGTCAGATCTCTCACCACCCGGTTAGATCTCGCGGTCAGTGGCATTGCCACGTCGGATCTCAAACCCGTGGACACCGAAAGCTTCGCTACCGACGAGTTCGAGGGCCCCGTTGTTTCGGGGACGAGCCAGGGCAGCGGTGAGTACTTCTCCCGAGTCGGAATCGGCAGACCGCCGAGCCAGGTCTACGTGGTGCTCGACACGGGCAGTGACGTCAGCTGGGTCCAGTGCGCGCCGTGCGCCGACTGTTACCAGCAAGCCGACCCGATTTTCGAGCCGACTTCCTCGGCTTCCTTCGCGCAGCTGTCGTGCGACGCCGCGCGGTGCAAGTCCCTCGACGTGTTCGAATGCCGCAACAACACGTGTCTCTACGAGGTGGCCTACGGGGACGGGTCCTACACCGTCGGCGACTTCGTGACGGAGACGATCAGCATCGGCGGGGAATCTTTGAGCGACGTCGCCATTGGATGCGGGCACAACAACGAAGGCCTCTTCGTCGGCGCGGCTGGCTTGCTCGGACTCGGCGGAGGGCCGTTGTCGTTTCCTTCGCAGCTAAACGCCACGTCGTTTTCGTACTGCCTCGTCGATCGTGACTCCGACTCCACCTCGACTCTCGAGTTCAACACAGCACTGCCTCACAACGCCGTAACGGCTCCGTTACGGCGCAACAGCCAGCTCAACACTTTCTACTACGTCGGGCTGACCGGGATCAGCGTCGGCGGGGATATGTTAAAGATTCCGGCCAACGCGTTCGAGATAGACGAAAGCGGGAACGGCGGGATCATCATCGACTCCGGGACGGCGGTGACGAGGCTGCAGACGGAGACGTACGACGCGCTGCGGAGCGCGTTCGTGAACATGACGAAGGGGCTGGAGTCGACGAGCGGGGTGGCGCTGTTCGACGCGTGCTACGACTTGTCGAAGATTGAGAGCGTGGACGTGCCGGCATTGTCGTTTCACTTCGAGGACGGGAAGGAGCTGCCGTTGCCGGCGAAGAACTACTTGATTCCGGTGGACTCGCAGGGGACGTTCTGCTTCGCGTTCGCGCCGACGAGCGGGGCGCTGTCGATAATTGGGAACGTGCAGCAGCAAGGGACACGTGTCAGTTTCGACATAGCTAATTCGGTGGTTGGATTCTCGGCCGACCAATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

482

Amino Acids

50.79

Weight (kDa)

4.68

Isoelectric Point (pI)

36.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 145 - 477 1.3e-18 Eukaryotic aspartyl protease
TAXi_N PF14543 146 - 304 1.1e-47 Xylanase inhibitor N-terminal
TAXi_C PF14541 327 - 477 1.6e-38 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014902)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25510
fragaria_vesca FvH4_4g27770
malus_domestica MD13G1044900.v1.1
prunus_persica Prupe.1G307100_v2.0.a1
pyrus_communis pycom13g03970 pycom16g04080
rosa_chinensis RchiOBHm_Chr4g0435631
rosa_laevigata RLG00000006575
rosa_multiflora Rmu_sc0000051.1_g000004
rosa_roxburghii Rroxscaffold_5G00376850
rosa_rugosa Rorug04G0287400
rosa_samantha Rh4AG342600 Rh4BG350900 Rh4CG365600 Rh4DG345300
rosa_wichuraiana Rw4G029900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 102, 507, 750
AccBSI CCGCTC 2 cut(s) 1176, 1347
AccI GTMKAC 2 cut(s) 477, 1169
AclWI GGATC 3 cut(s) 357, 1007, 1079
AcoI YGGCCR 2 cut(s) 1032, 1431
AcvI CACGTG 2 cut(s) 654, 1391
AcyI GRCGYC 4 cut(s) 99, 504, 606, 747
AfaI GTAC 3 cut(s) 437, 866, 1121
AfiI CCNNNNNNNGG 1 cut(s) 1346
AflIII ACRYGT 7 cut(s) 630, 651, 653, 1038, 1194, 1388, 1390
AgsI TTSAA 1 cut(s) 913
AhdI GACNNNNNGTC 1 cut(s) 253
AjiI CACGTC 4 cut(s) 345, 631, 855, 1231
AjnI CCWGG 2 cut(s) 420, 471
Alw21I GWGCWC 2 cut(s) 96, 489
Alw26I GTCTC 3 cut(s) 662, 707, 1109
AlwI GGATC 3 cut(s) 357, 1007, 1079
Ama87I CYCGRG 4 cut(s) 275, 291, 444, 905
AoxI GGCC 8 cut(s) 117, 288, 397, 669, 778, 821, 1032, 1431
ApaI GGGCCC 1 cut(s) 401
ApeKI GCWGC 8 cut(s) 426, 592, 841, 1105, 1129, 1270, 1375, 1378
ArsI GACNNNNNNTTYG 2 cut(s) 545, 577
AspS9I GGNCC 6 cut(s) 289, 397, 398, 514, 684, 821
AsuC2I CCSGG 3 cut(s) 313, 997, 1087
AsuHPI GGTGA 3 cut(s) 298, 443, 1108
AsuII TTCGAA 1 cut(s) 636
AvaI CYCGRG 4 cut(s) 275, 291, 444, 905
AvaII GGWCC 2 cut(s) 514, 684
BaeGI GKGCMC 2 cut(s) 401, 768
BanII GRGCYC 1 cut(s) 401
BbrPI CACGTG 2 cut(s) 654, 1391
Bbv12I GWGCWC 2 cut(s) 96, 489
BbvI GCAGC 8 cut(s) 438, 604, 853, 1092, 1116, 1257, 1387, 1390
BceAI ACGGC 9 cut(s) 116, 511, 808, 920, 957, 968, 1081, 1107, 1258
BcgI CGANNNNNNTGC 2 cut(s) 607, 641
BciT130I CCWGG 2 cut(s) 422, 473
BcnI CCSGG 3 cut(s) 313, 997, 1087
BcoDI GTCTC 3 cut(s) 662, 707, 1109
BfaI CTAG 1 cut(s) 1444
BfmI CTRYAG 1 cut(s) 1106
BfoI RGCGCY 2 cut(s) 1187, 1355
BglI GCCNNNNNGGC 1 cut(s) 941
BglII AGATCT 2 cut(s) 299, 319
BmcAI AGTACT 1 cut(s) 437
Bme1390I CCNGG 5 cut(s) 313, 422, 473, 997, 1087
Bme18I GGWCC 2 cut(s) 514, 684
BmeRI GACNNNNNGTC 1 cut(s) 253
BmeT110I CYCGRG 4 cut(s) 275, 291, 444, 905
BmgBI CACGTC 4 cut(s) 345, 631, 855, 1231
BmgT120I GGNCC 6 cut(s) 289, 397, 398, 514, 684, 821
BmiI GGNNCC 6 cut(s) 7, 399, 400, 515, 685, 945
BmrFI CCNGG 5 cut(s) 313, 422, 473, 997, 1087
BmsI GCATC 2 cut(s) 732, 749
BoxI GACNNNNGTC 1 cut(s) 1391
BplI GAGNNNNNCTC 2 cut(s) 425, 457
BpmI CTGGAG 1 cut(s) 1184
Bpu14I TTCGAA 1 cut(s) 636
BpuMI CCSGG 3 cut(s) 313, 997, 1087
BsaAI YACGTR 3 cut(s) 481, 654, 1391
BsaBI GATNNNNATC 2 cut(s) 722, 1076
BsaHI GRCGYC 4 cut(s) 99, 504, 606, 747
BsaJI CCNNGG 3 cut(s) 360, 421, 576
BsaWI WCCGGW 1 cut(s) 1300
BsaXI ACNNNNNCTCC 4 cut(s) 875, 905, 1259, 1289
Bsc4I CCNNNNNNNGG 1 cut(s) 1346
Bse118I RCCGGY 2 cut(s) 1234, 1279
Bse1I ACTGG 1 cut(s) 517
Bse3DI GCAATG 1 cut(s) 336
Bse8I GATNNNNATC 2 cut(s) 722, 1076
BseBI CCWGG 2 cut(s) 422, 473
BseDI CCNNGG 3 cut(s) 360, 421, 576
BseGI GGATG 1 cut(s) 764
BseJI GATNNNNATC 2 cut(s) 722, 1076
BseLI CCNNNNNNNGG 1 cut(s) 1346
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 2 cut(s) 176, 195
BseNI ACTGG 1 cut(s) 517
BsePI GCGCGC 1 cut(s) 522
BseRI GAGGAG 1 cut(s) 26
BseSI GKGCMC 2 cut(s) 401, 768
BseX3I CGGCCG 1 cut(s) 1431
BseXI GCAGC 8 cut(s) 438, 604, 853, 1092, 1116, 1257, 1387, 1390
BseYI CCCAGC 1 cut(s) 510
BsgI GTGCAG 1 cut(s) 1394
Bsh1285I CGRYCG 2 cut(s) 880, 1434
BshFI GGCC 8 cut(s) 119, 290, 399, 671, 780, 823, 1034, 1433
BsiEI CGRYCG 2 cut(s) 880, 1434
BsiHKAI GWGCWC 2 cut(s) 96, 489
BsiHKCI CYCGRG 4 cut(s) 275, 291, 444, 905
BsiSI CCGG 7 cut(s) 313, 996, 1031, 1086, 1235, 1280, 1301
BsiWI CGTACG 1 cut(s) 1119
BslFI GGGAC 6 cut(s) 427, 607, 692, 1102, 1330, 1399
BslI CCNNNNNNNGG 1 cut(s) 1346
BsmAI GTCTC 3 cut(s) 662, 707, 1109
BsmBI CGTCTC 2 cut(s) 707, 1109
BsmFI GGGAC 6 cut(s) 427, 607, 692, 1102, 1330, 1399
BsmI GAATGC 2 cut(s) 235, 644
BsnI GGCC 8 cut(s) 119, 290, 399, 671, 780, 823, 1034, 1433
BsoBI CYCGRG 4 cut(s) 275, 291, 444, 905
Bsp119I TTCGAA 1 cut(s) 636
Bsp120I GGGCCC 1 cut(s) 397
Bsp1286I GDGCHC 4 cut(s) 96, 401, 489, 768
Bsp143I GATC 7 cut(s) 299, 319, 349, 717, 877, 999, 1071
BspANI GGCC 8 cut(s) 119, 290, 399, 671, 780, 823, 1034, 1433
BspCNI CTCAG 2 cut(s) 175, 194
BspLI GGNNCC 6 cut(s) 7, 399, 400, 515, 685, 945
BspMAI CTGCAG 1 cut(s) 1110
BspPI GGATC 3 cut(s) 357, 1007, 1079
BspT104I TTCGAA 1 cut(s) 636
BsrBI CCGCTC 2 cut(s) 1176, 1347
BsrDI GCAATG 1 cut(s) 336
BsrFI RCCGGY 2 cut(s) 1234, 1279
BsrI ACTGG 1 cut(s) 517
BssAI RCCGGY 2 cut(s) 1234, 1279
BssECI CCNNGG 3 cut(s) 360, 421, 576
BssHII GCGCGC 1 cut(s) 522
BssMI GATC 7 cut(s) 299, 319, 349, 717, 877, 999, 1071
BssNI GRCGYC 4 cut(s) 99, 504, 606, 747
Bst2UI CCWGG 2 cut(s) 422, 473
Bst4CI ACNGT 4 cut(s) 91, 152, 539, 694
Bst6I CTCTTC 3 cut(s) 196, 207, 788
BstACI GRCGYC 4 cut(s) 99, 504, 606, 747
BstBAI YACGTR 3 cut(s) 481, 654, 1391
BstBI TTCGAA 1 cut(s) 636
BstC8I GCNNGC 9 cut(s) 524, 549, 764, 799, 803, 965, 1198, 1236, 1281
BstDEI CTNAG 2 cut(s) 162, 181
BstDSI CCRYGG 1 cut(s) 360
BstF5I GGATG 1 cut(s) 764
BstH2I RGCGCY 2 cut(s) 1187, 1355
BstKTI GATC 7 cut(s) 302, 322, 352, 720, 880, 1002, 1074
BstMAI GTCTC 3 cut(s) 662, 707, 1109
BstMBI GATC 7 cut(s) 299, 319, 349, 717, 877, 999, 1071
BstMCI CGRYCG 2 cut(s) 880, 1434
BstMWI GCNNNNNNNGC 8 cut(s) 426, 528, 530, 587, 798, 941, 1065, 1276
BstNI CCWGG 2 cut(s) 422, 473
BstPAI GACNNNNGTC 1 cut(s) 1391
BstSCI CCNGG 5 cut(s) 311, 420, 471, 995, 1085
BstSFI CTRYAG 1 cut(s) 1106
BstSLI GKGCMC 2 cut(s) 401, 768
BstV1I GCAGC 8 cut(s) 438, 604, 853, 1092, 1116, 1257, 1387, 1390
BstX2I RGATCY 3 cut(s) 299, 319, 349
BstYI RGATCY 3 cut(s) 299, 319, 349
BstZI CGGCCG 1 cut(s) 1431
BsuRI GGCC 8 cut(s) 119, 290, 399, 671, 780, 823, 1034, 1433
BtgI CCRYGG 1 cut(s) 360
BtrI CACGTC 4 cut(s) 345, 631, 855, 1231
BtsCI GGATG 1 cut(s) 764
BtsI GCAGTG 2 cut(s) 505, 920
BtsIMutI CAGTG 6 cut(s) 157, 170, 337, 505, 524, 920
Cac8I GCNNGC 9 cut(s) 524, 549, 764, 799, 803, 965, 1198, 1236, 1281
Cfr10I RCCGGY 2 cut(s) 1234, 1279
Cfr13I GGNCC 6 cut(s) 289, 397, 398, 514, 684, 821
CseI GACGC 4 cut(s) 614, 994, 1133, 1202
Csp6I GTAC 3 cut(s) 436, 865, 1120
CviAII CATG 1 cut(s) 1150
CviQI GTAC 3 cut(s) 436, 865, 1120
DdeI CTNAG 2 cut(s) 162, 181
DpnI GATC 7 cut(s) 301, 321, 351, 719, 879, 1001, 1073
DpnII GATC 7 cut(s) 299, 319, 349, 717, 877, 999, 1071
DriI GACNNNNNGTC 1 cut(s) 253
EaeI YGGCCR 2 cut(s) 1032, 1431
EagI CGGCCG 1 cut(s) 1431
Eam1104I CTCTTC 3 cut(s) 196, 207, 788
Eam1105I GACNNNNNGTC 1 cut(s) 253
EarI CTCTTC 3 cut(s) 196, 207, 788
EciI GGCGGA 1 cut(s) 831
EclXI CGGCCG 1 cut(s) 1431
Eco147I AGGCCT 1 cut(s) 780
Eco24I GRGCYC 1 cut(s) 401
Eco47I GGWCC 2 cut(s) 514, 684
Eco52I CGGCCG 1 cut(s) 1431
Eco72I CACGTG 2 cut(s) 654, 1391
Eco88I CYCGRG 4 cut(s) 275, 291, 444, 905
EcoO109I RGGNCCY 3 cut(s) 397, 398, 684
EcoRII CCWGG 2 cut(s) 420, 471
EcoT38I GRGCYC 1 cut(s) 401
Esp3I CGTCTC 2 cut(s) 707, 1109
FaeI CATG 1 cut(s) 1153
FaiI YATR 3 cut(s) 1019, 1151, 1406
FalI AAGNNNNNCTT 2 cut(s) 1277, 1309
FaqI GGGAC 6 cut(s) 427, 607, 692, 1102, 1330, 1399
FatI CATG 1 cut(s) 1149
FauI CCCGC 9 cut(s) 65, 224, 722, 755, 1004, 1052, 1061, 1169, 1340
FblI GTMKAC 2 cut(s) 477, 1169
FokI GGATG 1 cut(s) 771
FriOI GRGCYC 1 cut(s) 401
FspBI CTAG 1 cut(s) 1444
GsaI CCCAGC 1 cut(s) 514
GsuI CTGGAG 1 cut(s) 1184
HaeII RGCGCY 2 cut(s) 1187, 1355
HaeIII GGCC 8 cut(s) 119, 290, 399, 671, 780, 823, 1034, 1433
HapII CCGG 7 cut(s) 313, 996, 1031, 1086, 1235, 1280, 1301
HgaI GACGC 4 cut(s) 614, 994, 1133, 1202
Hin1I GRCGYC 4 cut(s) 99, 504, 606, 747
Hin1II CATG 1 cut(s) 1153
HincII GTYRAC 1 cut(s) 1170
HindII GTYRAC 1 cut(s) 1170
HindIII AAGCTT 1 cut(s) 373
HpaII CCGG 7 cut(s) 313, 996, 1031, 1086, 1235, 1280, 1301
HphI GGTGA 3 cut(s) 298, 443, 1108
Hpy166II GTNNAC 6 cut(s) 364, 478, 1147, 1170, 1228, 1306
Hpy188I TCNGA 5 cut(s) 299, 349, 452, 809, 889
Hpy188III TCNNGA 6 cut(s) 444, 706, 881, 905, 1045, 1144
Hpy8I GTNNAC 6 cut(s) 364, 478, 1147, 1170, 1228, 1306
HpyAV CCTTC 5 cut(s) 595, 770, 846, 1150, 1258
HpyCH4III ACNGT 4 cut(s) 91, 152, 539, 694
HpyCH4V TGCA 3 cut(s) 618, 1108, 1375
HpyF10VI GCNNNNNNNGC 8 cut(s) 426, 528, 530, 587, 798, 941, 1065, 1276
HpyF3I CTNAG 2 cut(s) 162, 181
Hsp92I GRCGYC 4 cut(s) 99, 504, 606, 747
Hsp92II CATG 1 cut(s) 1153
KroI GCCGGC 2 cut(s) 1234, 1279
KroNI GCCGGC 2 cut(s) 1236, 1281
Kzo9I GATC 7 cut(s) 299, 319, 349, 717, 877, 999, 1071
LmnI GCTCC 4 cut(s) 155, 949, 1134, 1267
Lsp1109I GCAGC 8 cut(s) 438, 604, 853, 1092, 1116, 1257, 1387, 1390
LweI GCATC 2 cut(s) 732, 749
MaeI CTAG 1 cut(s) 1444
MaeIII GTNAC 7 cut(s) 500, 539, 706, 881, 937, 948, 1096
MalI GATC 7 cut(s) 301, 321, 351, 719, 879, 1001, 1073
MbiI CCGCTC 2 cut(s) 1176, 1347
MboI GATC 7 cut(s) 299, 319, 349, 717, 877, 999, 1071
MboII GAAGA 5 cut(s) 183, 194, 775, 1225, 1297
MflI RGATCY 3 cut(s) 299, 319, 349
MhlI GDGCHC 4 cut(s) 96, 401, 489, 768
MluCI AATT 2 cut(s) 1362, 1411
MluI ACGCGT 2 cut(s) 1038, 1194
MmeI TCCRAC 4 cut(s) 327, 430, 912, 1402
MroNI GCCGGC 2 cut(s) 1234, 1279
MseI TTAA 1 cut(s) 1022
MspA1I CMGCKG 3 cut(s) 429, 510, 595
MspI CCGG 7 cut(s) 313, 996, 1031, 1086, 1235, 1280, 1301
MspR9I CCNGG 5 cut(s) 313, 422, 473, 997, 1087
Mva1269I GAATGC 2 cut(s) 235, 644
MvaI CCWGG 2 cut(s) 422, 473
MwoI GCNNNNNNNGC 8 cut(s) 426, 528, 530, 587, 798, 941, 1065, 1276
NaeI GCCGGC 2 cut(s) 1236, 1281
NciI CCSGG 3 cut(s) 313, 997, 1087
NdeII GATC 7 cut(s) 299, 319, 349, 717, 877, 999, 1071
NgoMIV GCCGGC 2 cut(s) 1234, 1279
NlaIII CATG 1 cut(s) 1153
NlaIV GGNNCC 6 cut(s) 7, 399, 400, 515, 685, 945
NmeAIII GCCGAG 5 cut(s) 266, 492, 557, 792, 1409
NmuCI GTSAC 4 cut(s) 500, 706, 881, 1096
NspV TTCGAA 1 cut(s) 636
PaeR7I CTCGAG 1 cut(s) 905
PauI GCGCGC 1 cut(s) 522
PceI AGGCCT 1 cut(s) 780
PctI GAATGC 2 cut(s) 235, 644
PdiI GCCGGC 2 cut(s) 1236, 1281
PfeI GAWTC 5 cut(s) 453, 734, 1027, 1297, 1425
Pfl23II CGTACG 1 cut(s) 1119
PfoI TCCNGGA 1 cut(s) 1085
Ple19I CGATCG 1 cut(s) 880
PmaCI CACGTG 2 cut(s) 654, 1391
PmlI CACGTG 2 cut(s) 654, 1391
Ppu21I YACGTR 3 cut(s) 481, 654, 1391
PpuMI RGGWCCY 1 cut(s) 684
PshAI GACNNNNGTC 1 cut(s) 1391
Psp5II RGGWCCY 1 cut(s) 684
Psp6I CCWGG 2 cut(s) 420, 471
PspCI CACGTG 2 cut(s) 654, 1391
PspFI CCCAGC 1 cut(s) 510
PspGI CCWGG 2 cut(s) 420, 471
PspLI CGTACG 1 cut(s) 1119
PspN4I GGNNCC 6 cut(s) 7, 399, 400, 515, 685, 945
PspOMI GGGCCC 1 cut(s) 397
PspPI GGNCC 6 cut(s) 289, 397, 398, 514, 684, 821
PspPPI RGGWCCY 1 cut(s) 684
PstI CTGCAG 1 cut(s) 1110
PsuI RGATCY 3 cut(s) 299, 319, 349
PteI GCGCGC 1 cut(s) 522
PvuI CGATCG 1 cut(s) 880
PvuII CAGCTG 2 cut(s) 510, 595
RsaI GTAC 3 cut(s) 437, 866, 1121
RsaNI GTAC 3 cut(s) 436, 865, 1120
SalI GTCGAC 1 cut(s) 1168
SaqAI TTAA 1 cut(s) 1022
Sau3AI GATC 7 cut(s) 299, 319, 349, 717, 877, 999, 1071
Sau96I GGNCC 6 cut(s) 289, 397, 398, 514, 684, 821
ScaI AGTACT 1 cut(s) 437
ScrFI CCNGG 5 cut(s) 313, 422, 473, 997, 1087
SduI GDGCHC 4 cut(s) 96, 401, 489, 768
SfaNI GCATC 2 cut(s) 732, 749
SfcI CTRYAG 1 cut(s) 1106
Sfr274I CTCGAG 1 cut(s) 905
SfuI TTCGAA 1 cut(s) 636
SinI GGWCC 2 cut(s) 514, 684
SlaI CTCGAG 1 cut(s) 905
SmlI CTYRAG 1 cut(s) 905
SmoI CTYRAG 1 cut(s) 905
Sse9I AATT 2 cut(s) 1362, 1411
SseBI AGGCCT 1 cut(s) 780
SspMI CTAG 1 cut(s) 1444
StuI AGGCCT 1 cut(s) 780
StyD4I CCNGG 5 cut(s) 311, 420, 471, 995, 1085
TaaI ACNGT 4 cut(s) 91, 152, 539, 694
TasI AATT 2 cut(s) 1362, 1411
TatI WGTACW 1 cut(s) 435
TauI GCSGC 4 cut(s) 107, 611, 645, 797
TfiI GAWTC 5 cut(s) 453, 734, 1027, 1297, 1425
Tru1I TTAA 1 cut(s) 1022
Tru9I TTAA 1 cut(s) 1022
TscAI CASTG 6 cut(s) 157, 177, 337, 505, 524, 927
TseFI GTSAC 4 cut(s) 500, 706, 881, 1096
TseI GCWGC 8 cut(s) 426, 592, 841, 1105, 1129, 1270, 1375, 1378
Tsp45I GTSAC 4 cut(s) 500, 706, 881, 1096
TspGWI ACGGA 3 cut(s) 725, 936, 1127
TspRI CASTG 6 cut(s) 157, 177, 337, 505, 524, 927
VpaK11BI GGWCC 2 cut(s) 514, 684
XhoI CTCGAG 1 cut(s) 905
XmiI GTMKAC 2 cut(s) 477, 1169
XspI CTAG 1 cut(s) 1444
ZraI GACGTC 3 cut(s) 100, 505, 748
ZrmI AGTACT 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.