AT1G26340

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012398
Physical Location & Seq
Forward (+)
9113864 .. 9114977
1114 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G26340.1

Sequence Viewer

Length: 408 bp
ATGCCGACACTCACAAAGCTTTATTCCATGGAAGAAGCCGCAACTCACAACAAGCAAGATGACTGCTGGGTCGTCATCGACGGCAAGGTCTATGATGTATCCTCTTATATGGATGAGCATCCTGGAGGAGATGATGTGCTTCTTGCTGTCGCAGGCAAAGATGCAACGGATGATTTTGAAGACGCAGGGCACAGCAAAGATGCTAGGGAACTTATGGAGAAGTATTTTATTGGCGAGCTAGATGAATCTTCTTTACCGGAAATACCTGAGCTTAAGATCTACAAGAAGGACCAGCCACAAGACTCTGTTCAGAAGCTTTTTGACTTGACAAAGCAGTATTGGGTTGTTCCTGTCTCCATTATCACCATCTCTGTAGCGGTTAGTGTCTTGTTCTCTCGCAAGACTTAA

Protein Analysis

135

Amino Acids

15.22

Weight (kDa)

4.49

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 10 - 81 6e-31 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26340
fragaria_vesca FvH4_4g29701
malus_domestica MD13G1059800.v1.1 MD13G1059900.v1.1 MD16G1059300.v1.1
prunus_persica Prupe.1G289700_v2.0.a1
pyrus_communis pycom13g05330
rosa_chinensis RchiOBHm_Chr4g0437951
rosa_laevigata RLG00000006399
rosa_multiflora Rmu_sc0007538.1_g000013 Rmu_sc0025141.1_g000004
rosa_roxburghii Rroxscaffold_5G00379110
rosa_rugosa Rorug04G0305800
rosa_samantha Rh4AG357400 Rh4BG368300 Rh4CG382400 Rh4DG363200
rosa_wichuraiana Rw0G011530 Rw0G016140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 68, 86
AciI CCGC 2 cut(s) 39, 377
AflII CTTAAG 1 cut(s) 272
AgsI TTSAA 1 cut(s) 179
AjnI CCWGG 1 cut(s) 121
AluBI AGCT 4 cut(s) 19, 238, 271, 316
AluI AGCT 4 cut(s) 19, 238, 271, 316
Alw26I GTCTC 1 cut(s) 358
AspS9I GGNCC 1 cut(s) 289
AsuHPI GGTGA 1 cut(s) 355
AvaII GGWCC 1 cut(s) 289
BaeGI GKGCMC 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 186
BccI CCATC 1 cut(s) 374
BceAI ACGGC 1 cut(s) 97
BciT130I CCWGG 1 cut(s) 123
BciVI GTATCC 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 358
BfaI CTAG 2 cut(s) 204, 239
BfmI CTRYAG 1 cut(s) 372
BfrI CTTAAG 1 cut(s) 272
BfuI GTATCC 1 cut(s) 109
BglII AGATCT 1 cut(s) 276
BisI GCNGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 40
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 1 cut(s) 289
BmgT120I GGNCC 1 cut(s) 289
BmrFI CCNGG 1 cut(s) 123
BmsI GCATC 3 cut(s) 127, 151, 190
BpiI GAAGAC 1 cut(s) 186
BpmI CTGGAG 1 cut(s) 144
Bpu10I CCTNAGC 1 cut(s) 267
BsaBI GATNNNNATC 1 cut(s) 117
BsaJI CCNNGG 1 cut(s) 27
BsaWI WCCGGW 1 cut(s) 256
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bse8I GATNNNNATC 1 cut(s) 117
BseBI CCWGG 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 3 cut(s) 118, 118, 175
BseJI GATNNNNATC 1 cut(s) 117
BseMII CTCAG 1 cut(s) 258
BseRI GAGGAG 1 cut(s) 141
BseSI GKGCMC 1 cut(s) 192
BseYI CCCAGC 1 cut(s) 66
BsiSI CCGG 1 cut(s) 257
BsmAI GTCTC 1 cut(s) 358
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 1 cut(s) 276
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 2 cut(s) 39, 377
BspCNI CTCAG 1 cut(s) 259
BspTI CTTAAG 1 cut(s) 272
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 1 cut(s) 276
BssT1I CCWWGG 1 cut(s) 27
Bst2UI CCWGG 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 272
BstC8I GCNNGC 2 cut(s) 154, 236
BstDEI CTNAG 1 cut(s) 267
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 3 cut(s) 118, 118, 175
BstKTI GATC 1 cut(s) 279
BstMAI GTCTC 1 cut(s) 358
BstMBI GATC 1 cut(s) 276
BstNI CCWGG 1 cut(s) 123
BstSCI CCNGG 1 cut(s) 121
BstSFI CTRYAG 1 cut(s) 372
BstSLI GKGCMC 1 cut(s) 192
BstV2I GAAGAC 1 cut(s) 186
BstX2I RGATCY 1 cut(s) 276
BstYI RGATCY 1 cut(s) 276
BsuI GTATCC 1 cut(s) 109
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 3 cut(s) 118, 118, 175
Cac8I GCNNGC 2 cut(s) 154, 236
Cfr13I GGNCC 1 cut(s) 289
CseI GACGC 1 cut(s) 191
CviAII CATG 1 cut(s) 28
CviJI RGCY 6 cut(s) 19, 38, 238, 271, 295, 316
CviKI_1 RGCY 6 cut(s) 19, 38, 238, 271, 295, 316
DdeI CTNAG 1 cut(s) 267
DpnI GATC 1 cut(s) 278
DpnII GATC 1 cut(s) 276
DrdI GACNNNNNNGTC 2 cut(s) 68, 86
DseDI GACNNNNNNGTC 2 cut(s) 68, 86
Eco130I CCWWGG 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 289
EcoRII CCWGG 1 cut(s) 121
EcoT14I CCWWGG 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 1 cut(s) 31
FaiI YATR 5 cut(s) 29, 93, 108, 110, 215
FatI CATG 1 cut(s) 27
Fnu4HI GCNGC 1 cut(s) 39
FokI GGATG 3 cut(s) 105, 125, 182
Fsp4HI GCNGC 1 cut(s) 39
FspBI CTAG 2 cut(s) 204, 239
GluI GCNGC 1 cut(s) 39
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 1 cut(s) 144
HapII CCGG 1 cut(s) 257
HgaI GACGC 1 cut(s) 191
Hin1II CATG 1 cut(s) 31
HindIII AAGCTT 2 cut(s) 17, 314
HinfI GANTC 2 cut(s) 245, 302
HpaII CCGG 1 cut(s) 257
HphI GGTGA 1 cut(s) 355
Hpy188I TCNGA 1 cut(s) 312
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 1 cut(s) 280
HpyCH4V TGCA 1 cut(s) 164
HpyF3I CTNAG 1 cut(s) 267
Hsp92II CATG 1 cut(s) 31
Kzo9I GATC 1 cut(s) 276
LpnPI CCDG 9 cut(s) 52, 108, 135, 138, 171, 270, 279, 305, 363
LweI GCATC 3 cut(s) 127, 151, 190
MaeI CTAG 2 cut(s) 204, 239
MalI GATC 1 cut(s) 278
MboI GATC 1 cut(s) 276
MboII GAAGA 3 cut(s) 44, 191, 240
MflI RGATCY 1 cut(s) 276
MhlI GDGCHC 1 cut(s) 192
MlyI GAGTC 1 cut(s) 296
MnlI CCTC 2 cut(s) 112, 119
MseI TTAA 2 cut(s) 273, 406
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 1 cut(s) 257
MspR9I CCNGG 1 cut(s) 123
MvaI CCWGG 1 cut(s) 123
NcoI CCATGG 1 cut(s) 27
NdeII GATC 1 cut(s) 276
NlaIII CATG 1 cut(s) 31
PfeI GAWTC 1 cut(s) 245
PfoI TCCNGGA 1 cut(s) 121
PkrI GCNGC 1 cut(s) 40
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
Psp6I CCWGG 1 cut(s) 121
PspFI CCCAGC 1 cut(s) 66
PspGI CCWGG 1 cut(s) 121
PspPI GGNCC 1 cut(s) 289
PsuI RGATCY 1 cut(s) 276
SaqAI TTAA 2 cut(s) 273, 406
SatI GCNGC 1 cut(s) 39
Sau3AI GATC 1 cut(s) 276
Sau96I GGNCC 1 cut(s) 289
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 1 cut(s) 192
SetI ASST 6 cut(s) 21, 90, 240, 268, 273, 318
SfaNI GCATC 3 cut(s) 127, 151, 190
SfcI CTRYAG 1 cut(s) 372
SinI GGWCC 1 cut(s) 289
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
SsiI CCGC 2 cut(s) 39, 377
SspMI CTAG 2 cut(s) 204, 239
StyD4I CCNGG 1 cut(s) 121
StyI CCWWGG 1 cut(s) 27
TaqI TCGA 1 cut(s) 78
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 2 cut(s) 273, 406
Tru9I TTAA 2 cut(s) 273, 406
TspDTI ATGAA 1 cut(s) 258
TspGWI ACGGA 1 cut(s) 182
Vha464I CTTAAG 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 289
XspI CTAG 2 cut(s) 204, 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.