Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0012398

Physical Location & Seq

Genomic Coordinates Forward (+)

4196201 .. 4198214

Gene Structure 2014 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1059800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 405 bp

ATGCCGACGTTGACGAAGCTGTACACAATGCAAGAAGCCTCGCAGCACAACACCAAAGACAACTGCTGGGTCGTCATCGATGGCAAGGTGTACGATGTGTCAACCTATTTGGACGACCACCCTGGTGGAGACGATGTACTCCTGGATGCAACTGGAAGAGATGCCACCGAAGATTTTGAAGATGCCGGGCACAGCAAAACTGCGAGGGAGGAAATGGAAGCCTTCTGCATTGGAGAGCTTGACACCACCTCCTTGGATATTCCAGAGCTCGAGATTTTCTCCAAGAATCAGCCAACTGATTATCCAAAGAAGCTCGTGGACTTGACAAAGCGATACTGGGCTGTTCCTGCGGCTGTTGTTGGCATCTCCGTGGTTGTCGCGGTCTTGCACTTGCGCAAGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009941 GO:0010319 GO:0016020 GO:0019867 GO:0031090 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cyt-b5

Protein Analysis

135

Amino Acids

14.96

Weight (kDa)

4.69

Isoelectric Point (pI)

26.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cyt-b5	PF00173	9 - 81	3.6e-28	Cytochrome b5-like Heme/Steroid binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012398)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26340
fragaria_vesca	FvH4_4g29701
malus_domestica	MD13G1059800.v1.1 MD13G1059900.v1.1 MD16G1059300.v1.1
prunus_persica	Prupe.1G289700_v2.0.a1
pyrus_communis	pycom13g05330
rosa_chinensis	RchiOBHm_Chr4g0437951
rosa_laevigata	RLG00000006399
rosa_multiflora	Rmu_sc0007538.1_g000013 Rmu_sc0025141.1_g000004
rosa_roxburghii	Rroxscaffold_5G00379110
rosa_rugosa	Rorug04G0305800
rosa_samantha	Rh4AG357400 Rh4BG368300 Rh4CG382400 Rh4DG363200
rosa_wichuraiana	Rw0G011530 Rw0G016140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	395
AccII	CGCG	1 cut(s)	380
AciI	CCGC	2 cut(s)	350, 380
AfaI	GTAC	3 cut(s)	23, 92, 138
AgsI	TTSAA	1 cut(s)	179
AjnI	CCWGG	2 cut(s)	121, 141
AleI	CACNNNNGTG	1 cut(s)	123
AluBI	AGCT	4 cut(s)	19, 238, 268, 313
AluI	AGCT	4 cut(s)	19, 238, 268, 313
Alw21I	GWGCWC	1 cut(s)	270
Alw26I	GTCTC	1 cut(s)	123
Ama87I	CYCGRG	1 cut(s)	269
ApeKI	GCWGC	1 cut(s)	43
AspLEI	GCGC	1 cut(s)	396
AsuC2I	CCSGG	1 cut(s)	187
AvaI	CYCGRG	1 cut(s)	269
BaeGI	GKGCMC	1 cut(s)	192
BanII	GRGCYC	1 cut(s)	270
BauI	CACGAG	1 cut(s)	314
Bbv12I	GWGCWC	1 cut(s)	270
BbvI	GCAGC	1 cut(s)	55
BccI	CCATC	1 cut(s)	74
BciT130I	CCWGG	2 cut(s)	123, 143
BcnI	CCSGG	1 cut(s)	187
BcoDI	GTCTC	1 cut(s)	123
BisI	GCNGC	2 cut(s)	44, 351
BlsI	GCNGC	2 cut(s)	45, 352
Bme1390I	CCNGG	3 cut(s)	123, 143, 187
BmeT110I	CYCGRG	1 cut(s)	269
BmrFI	CCNGG	3 cut(s)	123, 143, 187
BmrI	ACTGGG	1 cut(s)	346
BmsI	GCATC	4 cut(s)	136, 151, 172, 372
BmuI	ACTGGG	1 cut(s)	346
BplI	GAGNNNNNCTC	2 cut(s)	263, 295
BpuMI	CCSGG	1 cut(s)	187
Bsa29I	ATCGAT	1 cut(s)	78
BsaJI	CCNNGG	3 cut(s)	121, 252, 369
BsaXI	ACNNNNNCTCC	4 cut(s)	120, 150, 233, 263
Bse1I	ACTGG	2 cut(s)	157, 341
BseBI	CCWGG	2 cut(s)	123, 143
BseCI	ATCGAT	1 cut(s)	78
BseDI	CCNNGG	3 cut(s)	121, 252, 369
BseGI	GGATG	1 cut(s)	151
BseNI	ACTGG	2 cut(s)	157, 341
BseSI	GKGCMC	1 cut(s)	192
BseXI	GCAGC	1 cut(s)	55
BseYI	CCCAGC	1 cut(s)	66
Bsh1236I	CGCG	1 cut(s)	380
BshVI	ATCGAT	1 cut(s)	78
BsiHKAI	GWGCWC	1 cut(s)	270
BsiHKCI	CYCGRG	1 cut(s)	269
BsiSI	CCGG	1 cut(s)	186
BsmAI	GTCTC	1 cut(s)	123
BsmBI	CGTCTC	1 cut(s)	123
BsoBI	CYCGRG	1 cut(s)	269
Bsp1286I	GDGCHC	2 cut(s)	192, 270
Bsp1407I	TGTACA	1 cut(s)	21
BspACI	CCGC	2 cut(s)	350, 380
BspDI	ATCGAT	1 cut(s)	78
BspFNI	CGCG	1 cut(s)	380
BsrGI	TGTACA	1 cut(s)	21
BsrI	ACTGG	2 cut(s)	157, 341
BssECI	CCNNGG	3 cut(s)	121, 252, 369
BssSI	CACGAG	1 cut(s)	314
BssT1I	CCWWGG	1 cut(s)	252
Bst2BI	CACGAG	1 cut(s)	314
Bst2UI	CCWGG	2 cut(s)	123, 143
Bst6I	CTCTTC	1 cut(s)	151
BstAUI	TGTACA	1 cut(s)	21
BstDSI	CCRYGG	1 cut(s)	369
BstF5I	GGATG	1 cut(s)	151
BstFNI	CGCG	1 cut(s)	380
BstHHI	GCGC	1 cut(s)	396
BstMAI	GTCTC	1 cut(s)	123
BstMWI	GCNNNNNNNGC	1 cut(s)	347
BstNI	CCWGG	2 cut(s)	123, 143
BstSCI	CCNGG	3 cut(s)	121, 141, 185
BstSLI	GKGCMC	1 cut(s)	192
BstUI	CGCG	1 cut(s)	380
BstV1I	GCAGC	1 cut(s)	55
BstXI	CCANNNNNNTGG	2 cut(s)	125, 253
Bsu15I	ATCGAT	1 cut(s)	78
BsuTUI	ATCGAT	1 cut(s)	78
BtgI	CCRYGG	1 cut(s)	369
BtsCI	GGATG	1 cut(s)	151
CfoI	GCGC	1 cut(s)	396
ClaI	ATCGAT	1 cut(s)	78
Csp6I	GTAC	3 cut(s)	22, 91, 137
CviJI	RGCY	9 cut(s)	19, 38, 221, 238, 268, 292, 313, 341, 353
CviKI_1	RGCY	9 cut(s)	19, 38, 221, 238, 268, 292, 313, 341, 353
CviQI	GTAC	3 cut(s)	22, 91, 137
Eam1104I	CTCTTC	1 cut(s)	151
EarI	CTCTTC	1 cut(s)	151
Ecl136II	GAGCTC	1 cut(s)	268
Eco130I	CCWWGG	1 cut(s)	252
Eco24I	GRGCYC	1 cut(s)	270
Eco53kI	GAGCTC	1 cut(s)	268
Eco88I	CYCGRG	1 cut(s)	269
EcoICRI	GAGCTC	1 cut(s)	268
EcoRII	CCWGG	2 cut(s)	121, 141
EcoT14I	CCWWGG	1 cut(s)	252
EcoT38I	GRGCYC	1 cut(s)	270
ErhI	CCWWGG	1 cut(s)	252
Esp3I	CGTCTC	1 cut(s)	123
Fnu4HI	GCNGC	2 cut(s)	44, 351
FokI	GGATG	1 cut(s)	158
FriOI	GRGCYC	1 cut(s)	270
Fsp4HI	GCNGC	2 cut(s)	44, 351
FspI	TGCGCA	1 cut(s)	395
GlaI	GCGC	1 cut(s)	395
GluI	GCNGC	2 cut(s)	44, 351
GsaI	CCCAGC	1 cut(s)	70
HapII	CCGG	1 cut(s)	186
HhaI	GCGC	1 cut(s)	396
Hin6I	GCGC	1 cut(s)	394
HinP1I	GCGC	1 cut(s)	394
HincII	GTYRAC	2 cut(s)	12, 102
HindII	GTYRAC	2 cut(s)	12, 102
HinfI	GANTC	1 cut(s)	286
HpaII	CCGG	1 cut(s)	186
Hpy166II	GTNNAC	5 cut(s)	12, 24, 91, 102, 319
Hpy188III	TCNNGA	2 cut(s)	263, 271
Hpy8I	GTNNAC	5 cut(s)	12, 24, 91, 102, 319
Hpy99I	CGWCG	1 cut(s)	10
HpyAV	CCTTC	1 cut(s)	232
HpyCH4IV	ACGT	1 cut(s)	8
HpyCH4V	TGCA	4 cut(s)	31, 149, 228, 388
HpyF10VI	GCNNNNNNNGC	1 cut(s)	347
HpySE526I	ACGT	1 cut(s)	8
HspAI	GCGC	1 cut(s)	394
Lsp1109I	GCAGC	1 cut(s)	55
LweI	GCATC	4 cut(s)	136, 151, 172, 372
MaeII	ACGT	1 cut(s)	8
MboII	GAAGA	3 cut(s)	168, 182, 191
MhlI	GDGCHC	2 cut(s)	192, 270
MnlI	CCTC	4 cut(s)	49, 198, 202, 259
MslI	CAYNNNNRTG	2 cut(s)	123, 368
MspI	CCGG	1 cut(s)	186
MspR9I	CCNGG	3 cut(s)	123, 143, 187
MvaI	CCWGG	2 cut(s)	123, 143
MvnI	CGCG	1 cut(s)	380
MwoI	GCNNNNNNNGC	1 cut(s)	347
NciI	CCSGG	1 cut(s)	187
NsbI	TGCGCA	1 cut(s)	395
OliI	CACNNNNGTG	1 cut(s)	123
PaeR7I	CTCGAG	1 cut(s)	269
PfeI	GAWTC	1 cut(s)	286
PfoI	TCCNGGA	1 cut(s)	141
PkrI	GCNGC	2 cut(s)	45, 352
Psp124BI	GAGCTC	1 cut(s)	270
Psp6I	CCWGG	2 cut(s)	121, 141
PspFI	CCCAGC	1 cut(s)	66
PspGI	CCWGG	2 cut(s)	121, 141
RsaI	GTAC	3 cut(s)	23, 92, 138
RsaNI	GTAC	3 cut(s)	22, 91, 137
RseI	CAYNNNNRTG	2 cut(s)	123, 368
SacI	GAGCTC	1 cut(s)	270
SatI	GCNGC	2 cut(s)	44, 351
ScrFI	CCNGG	3 cut(s)	123, 143, 187
SduI	GDGCHC	2 cut(s)	192, 270
SetI	ASST	8 cut(s)	11, 21, 90, 107, 240, 251, 270, 315
SfaNI	GCATC	4 cut(s)	136, 151, 172, 372
Sfr274I	CTCGAG	1 cut(s)	269
SlaI	CTCGAG	1 cut(s)	269
SmiMI	CAYNNNNRTG	2 cut(s)	123, 368
SmlI	CTYRAG	1 cut(s)	269
SmoI	CTYRAG	1 cut(s)	269
SsiI	CCGC	2 cut(s)	350, 380
SstI	GAGCTC	1 cut(s)	270
StyD4I	CCNGG	3 cut(s)	121, 141, 185
StyI	CCWWGG	1 cut(s)	252
TaiI	ACGT	1 cut(s)	11
TaqI	TCGA	2 cut(s)	78, 270
TatI	WGTACW	2 cut(s)	21, 136
TauI	GCSGC	1 cut(s)	353
TfiI	GAWTC	1 cut(s)	286
TseI	GCWGC	1 cut(s)	43
TspGWI	ACGGA	1 cut(s)	358
XhoI	CTCGAG	1 cut(s)	269

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.