FvH4_4g29701

cytochrome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012398
Physical Location & Seq
Forward (+)
29737979 .. 29739777
1799 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29701.t1

Sequence Viewer

Length: 393 bp
ATGCCGACGCTGACGAAGCTATACTCAATGAAAGAAGCCTCGCAGCACAACACCAAAGATGACTGCTGGATCGTCATCGACGGCAAGGTGTATGATGTTTCAACATATCTGGATGATCACCCTGGTGGAGATGATGTAATCCTTGCTACAACTGGCAAAGACGCCACTGATGAATTTGAAGATGCTGGGCATAGCAAAGATGCAAAGGAGCTCATGGAAACCATGTGCATTGGAGAGCTCGACACAGACGCCTCTGCCTCAGCTGAACCTGAAACTCAACCAACTGGTTATCCTGAGAAGCTCATGACACTGACAAAGCAATACTGGGCTGTTCCTGCAGCTGTTGTTGGCATCTCTGCGGTGGTTGGCATCTTGTACTTGCGGAAGAAGTGA

Protein Analysis

131

Amino Acids

14.2

Weight (kDa)

4.49

Isoelectric Point (pI)

23.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 10 - 81 2.2e-30 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26340
fragaria_vesca FvH4_4g29701
malus_domestica MD13G1059800.v1.1 MD13G1059900.v1.1 MD16G1059300.v1.1
prunus_persica Prupe.1G289700_v2.0.a1
pyrus_communis pycom13g05330
rosa_chinensis RchiOBHm_Chr4g0437951
rosa_laevigata RLG00000006399
rosa_multiflora Rmu_sc0007538.1_g000013 Rmu_sc0025141.1_g000004
rosa_roxburghii Rroxscaffold_5G00379110
rosa_rugosa Rorug04G0305800
rosa_samantha Rh4AG357400 Rh4BG368300 Rh4CG382400 Rh4DG363200
rosa_wichuraiana Rw0G011530 Rw0G016140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 359, 382
AclWI GGATC 1 cut(s) 77
AcsI RAATTY 1 cut(s) 173
AcyI GRCGYC 2 cut(s) 162, 249
AfaI GTAC 1 cut(s) 377
AgsI TTSAA 2 cut(s) 102, 179
AjnI CCWGG 1 cut(s) 121
AleI CACNNNNGTG 1 cut(s) 123
AluBI AGCT 6 cut(s) 19, 211, 238, 263, 301, 341
AluI AGCT 6 cut(s) 19, 211, 238, 263, 301, 341
Alw21I GWGCWC 2 cut(s) 213, 240
AlwI GGATC 1 cut(s) 77
ApeKI GCWGC 2 cut(s) 43, 338
ApoI RAATTY 1 cut(s) 173
AsuHPI GGTGA 1 cut(s) 110
BanII GRGCYC 2 cut(s) 213, 240
Bbv12I GWGCWC 2 cut(s) 213, 240
BbvCI CCTCAGC 1 cut(s) 259
BbvI GCAGC 2 cut(s) 55, 350
BceAI ACGGC 1 cut(s) 97
BciT130I CCWGG 1 cut(s) 123
BclI TGATCA 1 cut(s) 115
BfmI CTRYAG 1 cut(s) 336
BisI GCNGC 2 cut(s) 44, 339
BlsI GCNGC 2 cut(s) 45, 340
Bme1390I CCNGG 1 cut(s) 123
BmrFI CCNGG 1 cut(s) 123
BmrI ACTGGG 1 cut(s) 334
BmsI GCATC 4 cut(s) 172, 190, 360, 378
BmuI ACTGGG 1 cut(s) 334
Bpu10I CCTNAGC 1 cut(s) 259
BsaBI GATNNNNATC 1 cut(s) 74
BsaHI GRCGYC 2 cut(s) 162, 249
BsaJI CCNNGG 1 cut(s) 121
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bse1I ACTGG 3 cut(s) 157, 289, 329
Bse8I GATNNNNATC 1 cut(s) 74
BseBI CCWGG 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 121
BseGI GGATG 1 cut(s) 118
BseJI GATNNNNATC 1 cut(s) 74
BseMII CTCAG 2 cut(s) 273, 285
BseNI ACTGG 3 cut(s) 157, 289, 329
BseXI GCAGC 2 cut(s) 55, 350
BseYI CCCAGC 1 cut(s) 185
BsiHKAI GWGCWC 2 cut(s) 213, 240
Bsp1286I GDGCHC 2 cut(s) 213, 240
Bsp143I GATC 2 cut(s) 69, 115
BspACI CCGC 2 cut(s) 359, 382
BspCNI CTCAG 2 cut(s) 272, 286
BspHI TCATGA 1 cut(s) 303
BspMAI CTGCAG 1 cut(s) 340
BspPI GGATC 1 cut(s) 77
BsrI ACTGG 3 cut(s) 157, 289, 329
BssECI CCNNGG 1 cut(s) 121
BssMI GATC 2 cut(s) 69, 115
BssNI GRCGYC 2 cut(s) 162, 249
Bst2UI CCWGG 1 cut(s) 123
BstACI GRCGYC 2 cut(s) 162, 249
BstDEI CTNAG 2 cut(s) 259, 294
BstF5I GGATG 1 cut(s) 118
BstKTI GATC 2 cut(s) 72, 118
BstMBI GATC 2 cut(s) 69, 115
BstMWI GCNNNNNNNGC 2 cut(s) 16, 335
BstNI CCWGG 1 cut(s) 123
BstSCI CCNGG 1 cut(s) 121
BstSFI CTRYAG 1 cut(s) 336
BstV1I GCAGC 2 cut(s) 55, 350
BtsCI GGATG 1 cut(s) 118
BtsIMutI CAGTG 2 cut(s) 165, 308
CciI TCATGA 1 cut(s) 303
CseI GACGC 3 cut(s) 16, 170, 257
Csp6I GTAC 1 cut(s) 376
CviAII CATG 3 cut(s) 214, 223, 304
CviJI RGCY 8 cut(s) 19, 38, 211, 238, 263, 301, 329, 341
CviKI_1 RGCY 8 cut(s) 19, 38, 211, 238, 263, 301, 329, 341
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 2 cut(s) 259, 294
DpnI GATC 2 cut(s) 71, 117
DpnII GATC 2 cut(s) 69, 115
Ecl136II GAGCTC 2 cut(s) 211, 238
Eco24I GRGCYC 2 cut(s) 213, 240
Eco53kI GAGCTC 2 cut(s) 211, 238
EcoICRI GAGCTC 2 cut(s) 211, 238
EcoRII CCWGG 1 cut(s) 121
EcoT38I GRGCYC 2 cut(s) 213, 240
FaeI CATG 3 cut(s) 217, 226, 307
FaiI YATR 7 cut(s) 22, 93, 106, 192, 215, 224, 305
FatI CATG 3 cut(s) 213, 222, 303
FbaI TGATCA 1 cut(s) 115
Fnu4HI GCNGC 2 cut(s) 44, 339
FokI GGATG 1 cut(s) 125
FriOI GRGCYC 2 cut(s) 213, 240
Fsp4HI GCNGC 2 cut(s) 44, 339
GluI GCNGC 2 cut(s) 44, 339
GsaI CCCAGC 1 cut(s) 189
HgaI GACGC 3 cut(s) 16, 170, 257
Hin1I GRCGYC 2 cut(s) 162, 249
Hin1II CATG 3 cut(s) 217, 226, 307
HphI GGTGA 1 cut(s) 110
Hpy188III TCNNGA 3 cut(s) 110, 293, 304
Hpy99I CGWCG 2 cut(s) 10, 83
HpyCH4V TGCA 3 cut(s) 203, 228, 338
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 335
HpyF3I CTNAG 2 cut(s) 259, 294
Hsp92I GRCGYC 2 cut(s) 162, 249
Hsp92II CATG 3 cut(s) 217, 226, 307
Ksp22I TGATCA 1 cut(s) 115
Kzo9I GATC 2 cut(s) 69, 115
LmnI GCTCC 1 cut(s) 208
Lsp1109I GCAGC 2 cut(s) 55, 350
LweI GCATC 4 cut(s) 172, 190, 360, 378
MalI GATC 2 cut(s) 71, 117
MboI GATC 2 cut(s) 69, 115
MboII GAAGA 1 cut(s) 191
MhlI GDGCHC 2 cut(s) 213, 240
MluCI AATT 1 cut(s) 173
MnlI CCTC 3 cut(s) 49, 262, 268
MslI CAYNNNNRTG 1 cut(s) 123
MspA1I CMGCKG 2 cut(s) 263, 341
MspR9I CCNGG 1 cut(s) 123
MvaI CCWGG 1 cut(s) 123
MwoI GCNNNNNNNGC 2 cut(s) 16, 335
NdeII GATC 2 cut(s) 69, 115
NlaIII CATG 3 cut(s) 217, 226, 307
OliI CACNNNNGTG 1 cut(s) 123
PagI TCATGA 1 cut(s) 303
PkrI GCNGC 2 cut(s) 45, 340
Psp124BI GAGCTC 2 cut(s) 213, 240
Psp6I CCWGG 1 cut(s) 121
PspFI CCCAGC 1 cut(s) 185
PspGI CCWGG 1 cut(s) 121
PstI CTGCAG 1 cut(s) 340
PvuII CAGCTG 2 cut(s) 263, 341
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
RseI CAYNNNNRTG 1 cut(s) 123
SacI GAGCTC 2 cut(s) 213, 240
SatI GCNGC 2 cut(s) 44, 339
Sau3AI GATC 2 cut(s) 69, 115
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 2 cut(s) 213, 240
SetI ASST 8 cut(s) 21, 90, 213, 240, 265, 271, 303, 343
SfaNI GCATC 4 cut(s) 172, 190, 360, 378
SfcI CTRYAG 1 cut(s) 336
SmiMI CAYNNNNRTG 1 cut(s) 123
Sse9I AATT 1 cut(s) 173
SsiI CCGC 2 cut(s) 359, 382
SstI GAGCTC 2 cut(s) 213, 240
StyD4I CCNGG 1 cut(s) 121
TaqI TCGA 2 cut(s) 78, 240
TasI AATT 1 cut(s) 173
TatI WGTACW 1 cut(s) 375
TscAI CASTG 2 cut(s) 172, 315
TseI GCWGC 2 cut(s) 43, 338
TspDTI ATGAA 2 cut(s) 44, 186
TspRI CASTG 2 cut(s) 172, 315
XapI RAATTY 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.