Rw0G016140

cytochrome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00751
OG0012398
Physical Location & Seq
Forward (+)
78246 .. 79807
1562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G016140.1

Sequence Viewer

Length: 399 bp
ATGCCGACGCTGACGAAGCTCTACACAATGCAAGAAGCCTCGCAGCACAACACCAAAGACGACTGCTGGATCGTCGTCGACGGCAAGGTGTACGATGTGTCAACATATTTGGATGATCACCCTGGTGGGGATGATGTAATCCTTGCTACAACTGGCAAAGATGCCACTGACGATTTTGAAGATGCTGGGCACAGCAAAAGTGCAAAGGAGCTCATGGAAACCATGTGCATTGGAGAGCTTGACACAGACACCTCCGCCTCAGCTGAACTTGAGAATCAACCAACTGGTTATCCGCAGAAGCTCATGACCCTGACAAAGCAATACTGGGCTGTTCCTGCGGCCGTTGTGGGCATCTCTGTGGTGGTTGGTTTCGTGTACTTGCGCAAGAAGAATACATGA

Protein Analysis

132

Amino Acids

14.48

Weight (kDa)

4.5

Isoelectric Point (pI)

16.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 9 - 81 1.1e-29 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26340
fragaria_vesca FvH4_4g29701
malus_domestica MD13G1059800.v1.1 MD13G1059900.v1.1 MD16G1059300.v1.1
prunus_persica Prupe.1G289700_v2.0.a1
pyrus_communis pycom13g05330
rosa_chinensis RchiOBHm_Chr4g0437951
rosa_laevigata RLG00000006399
rosa_multiflora Rmu_sc0007538.1_g000013 Rmu_sc0025141.1_g000004
rosa_roxburghii Rroxscaffold_5G00379110
rosa_rugosa Rorug04G0305800
rosa_samantha Rh4AG357400 Rh4BG368300 Rh4CG382400 Rh4DG363200
rosa_wichuraiana Rw0G011530 Rw0G016140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 383
AccI GTMKAC 1 cut(s) 78
AciI CCGC 3 cut(s) 255, 293, 338
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 1 cut(s) 339
AfaI GTAC 2 cut(s) 92, 377
AfiI CCNNNNNNNGG 1 cut(s) 127
AgsI TTSAA 1 cut(s) 179
AjnI CCWGG 1 cut(s) 121
AleI CACNNNNGTG 1 cut(s) 123
AluBI AGCT 5 cut(s) 19, 211, 238, 263, 301
AluI AGCT 5 cut(s) 19, 211, 238, 263, 301
Alw21I GWGCWC 1 cut(s) 213
AlwI GGATC 1 cut(s) 77
AoxI GGCC 1 cut(s) 339
ApeKI GCWGC 1 cut(s) 43
AspLEI GCGC 1 cut(s) 384
AsuHPI GGTGA 1 cut(s) 110
BaeGI GKGCMC 1 cut(s) 192
BanII GRGCYC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 213
BbvCI CCTCAGC 1 cut(s) 259
BbvI GCAGC 1 cut(s) 55
BceAI ACGGC 2 cut(s) 97, 326
BciT130I CCWGG 1 cut(s) 123
BclI TGATCA 1 cut(s) 115
BisI GCNGC 2 cut(s) 44, 339
BlsI GCNGC 2 cut(s) 45, 340
Bme1390I CCNGG 1 cut(s) 123
BmrFI CCNGG 1 cut(s) 123
BmrI ACTGGG 1 cut(s) 334
BmsI GCATC 3 cut(s) 151, 172, 360
BmuI ACTGGG 1 cut(s) 334
Bpu10I CCTNAGC 1 cut(s) 259
BpuEI CTTGAG 1 cut(s) 290
BsaJI CCNNGG 1 cut(s) 121
Bsc4I CCNNNNNNNGG 1 cut(s) 127
Bse1I ACTGG 3 cut(s) 157, 289, 329
BseBI CCWGG 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 121
BseGI GGATG 2 cut(s) 118, 136
BseLI CCNNNNNNNGG 1 cut(s) 127
BseMII CTCAG 1 cut(s) 273
BseNI ACTGG 3 cut(s) 157, 289, 329
BseSI GKGCMC 1 cut(s) 192
BseX3I CGGCCG 1 cut(s) 339
BseXI GCAGC 1 cut(s) 55
BseYI CCCAGC 1 cut(s) 185
Bsh1285I CGRYCG 1 cut(s) 342
BshFI GGCC 1 cut(s) 341
BsiEI CGRYCG 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 213
BslI CCNNNNNNNGG 1 cut(s) 127
BsnI GGCC 1 cut(s) 341
Bsp1286I GDGCHC 2 cut(s) 192, 213
Bsp143I GATC 2 cut(s) 69, 115
BspACI CCGC 3 cut(s) 255, 293, 338
BspANI GGCC 1 cut(s) 341
BspCNI CTCAG 1 cut(s) 272
BspHI TCATGA 1 cut(s) 303
BspPI GGATC 1 cut(s) 77
BsrI ACTGG 3 cut(s) 157, 289, 329
BssECI CCNNGG 1 cut(s) 121
BssMI GATC 2 cut(s) 69, 115
Bst2UI CCWGG 1 cut(s) 123
BstDEI CTNAG 1 cut(s) 259
BstF5I GGATG 2 cut(s) 118, 136
BstHHI GCGC 1 cut(s) 384
BstKTI GATC 2 cut(s) 72, 118
BstMBI GATC 2 cut(s) 69, 115
BstMCI CGRYCG 1 cut(s) 342
BstMWI GCNNNNNNNGC 2 cut(s) 16, 335
BstNI CCWGG 1 cut(s) 123
BstSCI CCNGG 1 cut(s) 121
BstSLI GKGCMC 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 55
BstZI CGGCCG 1 cut(s) 339
BsuRI GGCC 1 cut(s) 341
BtsCI GGATG 2 cut(s) 118, 136
BtsIMutI CAGTG 1 cut(s) 165
CciI TCATGA 1 cut(s) 303
CfoI GCGC 1 cut(s) 384
CseI GACGC 1 cut(s) 16
Csp6I GTAC 2 cut(s) 91, 376
CviAII CATG 4 cut(s) 214, 223, 304, 396
CviJI RGCY 8 cut(s) 19, 38, 211, 238, 263, 301, 329, 341
CviKI_1 RGCY 8 cut(s) 19, 38, 211, 238, 263, 301, 329, 341
CviQI GTAC 2 cut(s) 91, 376
DdeI CTNAG 1 cut(s) 259
DpnI GATC 2 cut(s) 71, 117
DpnII GATC 2 cut(s) 69, 115
EaeI YGGCCR 1 cut(s) 339
EagI CGGCCG 1 cut(s) 339
EciI GGCGGA 1 cut(s) 244
Ecl136II GAGCTC 1 cut(s) 211
EclXI CGGCCG 1 cut(s) 339
Eco24I GRGCYC 1 cut(s) 213
Eco52I CGGCCG 1 cut(s) 339
Eco53kI GAGCTC 1 cut(s) 211
EcoICRI GAGCTC 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 121
EcoT38I GRGCYC 1 cut(s) 213
FaeI CATG 4 cut(s) 217, 226, 307, 399
FaiI YATR 5 cut(s) 106, 215, 224, 305, 397
FatI CATG 4 cut(s) 213, 222, 303, 395
FbaI TGATCA 1 cut(s) 115
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 2 cut(s) 44, 339
FokI GGATG 2 cut(s) 125, 143
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 2 cut(s) 44, 339
FspI TGCGCA 1 cut(s) 383
GlaI GCGC 1 cut(s) 383
GluI GCNGC 2 cut(s) 44, 339
GsaI CCCAGC 1 cut(s) 189
HaeIII GGCC 1 cut(s) 341
HgaI GACGC 1 cut(s) 16
HhaI GCGC 1 cut(s) 384
Hin1II CATG 4 cut(s) 217, 226, 307, 399
Hin6I GCGC 1 cut(s) 382
HinP1I GCGC 1 cut(s) 382
HincII GTYRAC 2 cut(s) 79, 102
HindII GTYRAC 2 cut(s) 79, 102
HinfI GANTC 1 cut(s) 274
HphI GGTGA 1 cut(s) 110
Hpy166II GTNNAC 4 cut(s) 79, 91, 102, 376
Hpy188III TCNNGA 1 cut(s) 304
Hpy8I GTNNAC 4 cut(s) 79, 91, 102, 376
Hpy99I CGWCG 4 cut(s) 10, 77, 80, 83
HpyCH4V TGCA 3 cut(s) 31, 203, 228
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 335
HpyF3I CTNAG 1 cut(s) 259
Hsp92II CATG 4 cut(s) 217, 226, 307, 399
HspAI GCGC 1 cut(s) 382
Ksp22I TGATCA 1 cut(s) 115
Kzo9I GATC 2 cut(s) 69, 115
LmnI GCTCC 1 cut(s) 208
LpnPI CCDG 9 cut(s) 52, 108, 135, 138, 171, 270, 310, 323, 348
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 3 cut(s) 151, 172, 360
MalI GATC 2 cut(s) 71, 117
MboI GATC 2 cut(s) 69, 115
MboII GAAGA 1 cut(s) 191
MhlI GDGCHC 2 cut(s) 192, 213
MnlI CCTC 3 cut(s) 49, 262, 268
MslI CAYNNNNRTG 2 cut(s) 123, 356
MspA1I CMGCKG 1 cut(s) 263
MspR9I CCNGG 1 cut(s) 123
MvaI CCWGG 1 cut(s) 123
MwoI GCNNNNNNNGC 2 cut(s) 16, 335
NdeII GATC 2 cut(s) 69, 115
NlaIII CATG 4 cut(s) 217, 226, 307, 399
NsbI TGCGCA 1 cut(s) 383
OliI CACNNNNGTG 1 cut(s) 123
PagI TCATGA 1 cut(s) 303
PfeI GAWTC 1 cut(s) 274
PkrI GCNGC 2 cut(s) 45, 340
Psp124BI GAGCTC 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 121
PspFI CCCAGC 1 cut(s) 185
PspGI CCWGG 1 cut(s) 121
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 2 cut(s) 92, 377
RsaNI GTAC 2 cut(s) 91, 376
RseI CAYNNNNRTG 2 cut(s) 123, 356
SacI GAGCTC 1 cut(s) 213
SalI GTCGAC 1 cut(s) 77
SatI GCNGC 2 cut(s) 44, 339
Sau3AI GATC 2 cut(s) 69, 115
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 2 cut(s) 192, 213
SetI ASST 7 cut(s) 21, 90, 213, 240, 254, 265, 303
SfaNI GCATC 3 cut(s) 151, 172, 360
SgrDI CGTCGACG 1 cut(s) 77
SmiMI CAYNNNNRTG 2 cut(s) 123, 356
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
SsiI CCGC 3 cut(s) 255, 293, 338
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 1 cut(s) 121
TaqI TCGA 1 cut(s) 78
TatI WGTACW 1 cut(s) 375
TauI GCSGC 1 cut(s) 341
TfiI GAWTC 1 cut(s) 274
TscAI CASTG 1 cut(s) 172
TseI GCWGC 1 cut(s) 43
TspRI CASTG 1 cut(s) 172
XmiI GTMKAC 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.