MD16G1059300.v1.1

cytochrome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012398
Physical Location & Seq
Forward (+)
4235180 .. 4237091
1912 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1059300.v1.1.491

Sequence Viewer

Length: 405 bp
ATGCCGACTTTGACGAAGCTGTATACAATGCAAGAAGCGTCGCAGCACAACACCAAAGACAACTGCTGGGTCGTCATCGATGGCAAGGTGTATGATGTGTCAACCTATTTGGACGACCACCCTGGTGGAGACGACGTACTCCTGGCTGCAACTGGACGAGATGCCACCGAAGATTTTGAAGAAGCTGGGCACAGCAAAACTGCCAGGGAGGAAATGAGAGCCTTATGCATTGGAGAACTTGACACCACTTCCTCGGATATTCCAGAGCTAGAGATTTTCCCCGAGAATCAGCCACCGGGCTATCCCAAGAAGCTTGCCAATTTGACAAAGCGATACTGGGCTGTTCCGGTGGCTGTTGTTGGCATCTCCGTGGTTATTGGAGTCTTGTACTGGCGCAAGAAGTGA

Protein Analysis

135

Amino Acids

14.94

Weight (kDa)

4.87

Isoelectric Point (pI)

30.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 9 - 81 2.2e-27 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26340
fragaria_vesca FvH4_4g29701
malus_domestica MD13G1059800.v1.1 MD13G1059900.v1.1 MD16G1059300.v1.1
prunus_persica Prupe.1G289700_v2.0.a1
pyrus_communis pycom13g05330
rosa_chinensis RchiOBHm_Chr4g0437951
rosa_laevigata RLG00000006399
rosa_multiflora Rmu_sc0007538.1_g000013 Rmu_sc0025141.1_g000004
rosa_roxburghii Rroxscaffold_5G00379110
rosa_rugosa Rorug04G0305800
rosa_samantha Rh4AG357400 Rh4BG368300 Rh4CG382400 Rh4DG363200
rosa_wichuraiana Rw0G011530 Rw0G016140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 23
AfaI GTAC 2 cut(s) 138, 389
AgsI TTSAA 1 cut(s) 179
AjnI CCWGG 3 cut(s) 121, 141, 203
AleI CACNNNNGTG 1 cut(s) 123
AluBI AGCT 4 cut(s) 19, 185, 268, 313
AluI AGCT 4 cut(s) 19, 185, 268, 313
Alw26I GTCTC 1 cut(s) 123
Ama87I CYCGRG 1 cut(s) 281
ApeKI GCWGC 2 cut(s) 43, 146
AspLEI GCGC 1 cut(s) 396
AsuC2I CCSGG 1 cut(s) 297
AvaI CYCGRG 1 cut(s) 281
BaeGI GKGCMC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 55, 133
BccI CCATC 1 cut(s) 74
BciT130I CCWGG 3 cut(s) 123, 143, 205
BcnI CCSGG 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 123
BfaI CTAG 1 cut(s) 269
BisI GCNGC 2 cut(s) 44, 147
BlsI GCNGC 2 cut(s) 45, 148
Bme1390I CCNGG 4 cut(s) 123, 143, 205, 297
BmeT110I CYCGRG 1 cut(s) 281
BmrFI CCNGG 4 cut(s) 123, 143, 205, 297
BmrI ACTGGG 1 cut(s) 346
BmsI GCATC 2 cut(s) 151, 372
BmuI ACTGGG 1 cut(s) 346
BpuMI CCSGG 1 cut(s) 297
Bsa29I ATCGAT 1 cut(s) 78
BsaJI CCNNGG 4 cut(s) 121, 204, 252, 369
BsaWI WCCGGW 1 cut(s) 346
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bse1I ACTGG 3 cut(s) 157, 341, 395
BseBI CCWGG 3 cut(s) 123, 143, 205
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 4 cut(s) 121, 204, 252, 369
BseNI ACTGG 3 cut(s) 157, 341, 395
BseSI GKGCMC 1 cut(s) 192
BseXI GCAGC 2 cut(s) 55, 133
BseYI CCCAGC 2 cut(s) 66, 185
BshVI ATCGAT 1 cut(s) 78
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 2 cut(s) 296, 347
BsmAI GTCTC 1 cut(s) 123
BsmBI CGTCTC 1 cut(s) 123
BsoBI CYCGRG 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 192
BspDI ATCGAT 1 cut(s) 78
BsrI ACTGG 3 cut(s) 157, 341, 395
BssECI CCNNGG 4 cut(s) 121, 204, 252, 369
BssNAI GTATAC 1 cut(s) 24
Bst1107I GTATAC 1 cut(s) 24
Bst2UI CCWGG 3 cut(s) 123, 143, 205
BstC8I GCNNGC 1 cut(s) 315
BstDSI CCRYGG 1 cut(s) 369
BstHHI GCGC 1 cut(s) 396
BstMAI GTCTC 1 cut(s) 123
BstNI CCWGG 3 cut(s) 123, 143, 205
BstSCI CCNGG 4 cut(s) 121, 141, 203, 295
BstSLI GKGCMC 1 cut(s) 192
BstV1I GCAGC 2 cut(s) 55, 133
BstXI CCANNNNNNTGG 1 cut(s) 125
BstZ17I GTATAC 1 cut(s) 24
Bsu15I ATCGAT 1 cut(s) 78
BsuTUI ATCGAT 1 cut(s) 78
BtgI CCRYGG 1 cut(s) 369
Cac8I GCNNGC 1 cut(s) 315
CfoI GCGC 1 cut(s) 396
ClaI ATCGAT 1 cut(s) 78
CseI GACGC 1 cut(s) 27
Csp6I GTAC 2 cut(s) 137, 388
CviQI GTAC 2 cut(s) 137, 388
Eco88I CYCGRG 1 cut(s) 281
EcoRII CCWGG 3 cut(s) 121, 141, 203
EcoT22I ATGCAT 1 cut(s) 230
Esp3I CGTCTC 1 cut(s) 123
FaiI YATR 3 cut(s) 24, 93, 226
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 2 cut(s) 44, 147
Fsp4HI GCNGC 2 cut(s) 44, 147
FspBI CTAG 1 cut(s) 269
GlaI GCGC 1 cut(s) 395
GluI GCNGC 2 cut(s) 44, 147
GsaI CCCAGC 2 cut(s) 70, 189
HapII CCGG 2 cut(s) 296, 347
HgaI GACGC 1 cut(s) 27
HhaI GCGC 1 cut(s) 396
Hin6I GCGC 1 cut(s) 394
HinP1I GCGC 1 cut(s) 394
HincII GTYRAC 1 cut(s) 102
HindII GTYRAC 1 cut(s) 102
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 2 cut(s) 286, 381
HpaII CCGG 2 cut(s) 296, 347
Hpy166II GTNNAC 2 cut(s) 24, 102
Hpy188I TCNGA 1 cut(s) 256
Hpy188III TCNNGA 1 cut(s) 263
Hpy8I GTNNAC 2 cut(s) 24, 102
Hpy99I CGWCG 2 cut(s) 43, 137
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 3 cut(s) 31, 149, 228
HpySE526I ACGT 1 cut(s) 135
HspAI GCGC 1 cut(s) 394
Lsp1109I GCAGC 2 cut(s) 55, 133
LweI GCATC 2 cut(s) 151, 372
MaeI CTAG 1 cut(s) 269
MaeII ACGT 1 cut(s) 135
MboII GAAGA 2 cut(s) 182, 191
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 1 cut(s) 319
MlyI GAGTC 1 cut(s) 390
MnlI CCTC 2 cut(s) 202, 262
Mph1103I ATGCAT 1 cut(s) 230
MslI CAYNNNNRTG 2 cut(s) 123, 368
MspI CCGG 2 cut(s) 296, 347
MspR9I CCNGG 4 cut(s) 123, 143, 205, 297
MvaI CCWGG 3 cut(s) 123, 143, 205
NciI CCSGG 1 cut(s) 297
NsiI ATGCAT 1 cut(s) 230
OliI CACNNNNGTG 1 cut(s) 123
PfeI GAWTC 1 cut(s) 286
PkrI GCNGC 2 cut(s) 45, 148
PleI GAGTC 1 cut(s) 389
PpsI GAGTC 1 cut(s) 389
Psp6I CCWGG 3 cut(s) 121, 141, 203
PspFI CCCAGC 2 cut(s) 66, 185
PspGI CCWGG 3 cut(s) 121, 141, 203
RsaI GTAC 2 cut(s) 138, 389
RsaNI GTAC 2 cut(s) 137, 388
RseI CAYNNNNRTG 2 cut(s) 123, 368
SatI GCNGC 2 cut(s) 44, 147
SchI GAGTC 1 cut(s) 390
ScrFI CCNGG 4 cut(s) 123, 143, 205, 297
SduI GDGCHC 1 cut(s) 192
SetI ASST 7 cut(s) 21, 90, 107, 138, 187, 270, 315
SfaNI GCATC 2 cut(s) 151, 372
SmiMI CAYNNNNRTG 2 cut(s) 123, 368
Sse9I AATT 1 cut(s) 319
SspMI CTAG 1 cut(s) 269
StyD4I CCNGG 4 cut(s) 121, 141, 203, 295
TaiI ACGT 1 cut(s) 138
TaqI TCGA 1 cut(s) 78
TasI AATT 1 cut(s) 319
TatI WGTACW 1 cut(s) 387
TfiI GAWTC 1 cut(s) 286
TseI GCWGC 2 cut(s) 43, 146
TspGWI ACGGA 1 cut(s) 358
XmiI GTMKAC 1 cut(s) 23
XspI CTAG 1 cut(s) 269
Zsp2I ATGCAT 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.