AT1G27300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014595
Physical Location & Seq
Forward (+)
9483157 .. 9484368
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27300.1

Sequence Viewer

Length: 603 bp
ATGGAGTCCAGTGAGGATGTGGAGAATCTGAGTAGAGCTATTGAGAAGCTTCTTCATGAGAAGAGGAAGAGAGAAGCTTCTGGTGACGCCTTCATTGAAGACGCTGATGATCAGCTTTTCCTCTCTAGCCTTATCTCTCAGTTGGAATCACCAAACCCCAAGGGGAAAGAAGATGTCATAACCGGAGAAGAAGAAGAAAAAGAAGAAGAATCTGCAGATTCTTCACCATCCAAAGGAAAAAGCGAGGGCCAGAGACAATTGGAAGAAAGTATAGAAGAAATAGCTAAAGACATCAAGAAGGTGAAGAAGCAGAACAAAATAACCCATGTACTTCTCTCGGCTACGATCATCCTGACATTGGTTTGGCAGCTCTCTGAGTACTCCATGATTTTCATGTTGAAAGATAGAATAAGCCACCCAGTCAGATCCATCGGAGGTATGCTTAATGGAATGTTCAAAGGTAAGTTACGTCCAATCAAGAACCAACTTGCGGGGACTTCCAACTCCAATGACCAAAACAACCATGGGAATGGATCACACACTGGACCTCAACTTCAAGTGCCCGAGCTGTTGCGAGAATTCGGTTTCGACGATGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

22.42

Weight (kDa)

4.99

Isoelectric Point (pI)

57.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014595)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27300
fragaria_vesca FvH4_4g23570
malus_domestica MD16G1121100.v1.1
prunus_persica Prupe.1G224100_v2.0.a1
pyrus_communis pycom13g10540 pycom16g10370
rosa_chinensis RchiOBHm_Chr4g0429971
rosa_laevigata RLG00000007016
rosa_multiflora Rmu_co8323629.1_g000001 Rmu_ssc0000150.1_g000037
rosa_roxburghii Rroxscaffold_5G00371800
rosa_rugosa Rorug04G0239800
rosa_samantha Rh4AG296200 Rh4BG302700 Rh4CG318200 Rh4DG300000
rosa_wichuraiana Rw4G025660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 491
AclWI GGATC 2 cut(s) 420, 541
AcsI RAATTY 1 cut(s) 578
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 2 cut(s) 330, 380
AfiI CCNNNNNNNGG 3 cut(s) 233, 358, 490
AgsI TTSAA 4 cut(s) 98, 400, 457, 557
AluBI AGCT 7 cut(s) 38, 49, 77, 115, 284, 370, 568
AluI AGCT 7 cut(s) 38, 49, 77, 115, 284, 370, 568
Alw26I GTCTC 1 cut(s) 247
AlwI GGATC 2 cut(s) 420, 541
Ama87I CYCGRG 1 cut(s) 563
AoxI GGCC 1 cut(s) 247
ApeKI GCWGC 1 cut(s) 367
ApoI RAATTY 1 cut(s) 578
AspS9I GGNCC 2 cut(s) 247, 545
AsuHPI GGTGA 4 cut(s) 95, 141, 216, 313
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 1 cut(s) 545
BaeGI GKGCMC 1 cut(s) 564
BbsI GAAGAC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 379
BccI CCATC 2 cut(s) 235, 437
BclI TGATCA 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 247
BfaI CTAG 1 cut(s) 126
BfmI CTRYAG 1 cut(s) 213
BisI GCNGC 1 cut(s) 368
BlsI GCNGC 1 cut(s) 369
BmcAI AGTACT 1 cut(s) 380
Bme18I GGWCC 1 cut(s) 545
BmeT110I CYCGRG 1 cut(s) 563
BmgT120I GGNCC 2 cut(s) 247, 545
BmrI ACTGGG 1 cut(s) 413
BmuI ACTGGG 1 cut(s) 413
BpiI GAAGAC 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 2 cut(s) 159, 523
BsaWI WCCGGW 1 cut(s) 182
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 358, 490
Bse1I ACTGG 3 cut(s) 9, 419, 547
BseDI CCNNGG 2 cut(s) 159, 523
BseGI GGATG 3 cut(s) 22, 227, 348
BseLI CCNNNNNNNGG 3 cut(s) 233, 358, 490
BseMII CTCAG 3 cut(s) 20, 152, 366
BseNI ACTGG 3 cut(s) 9, 419, 547
BseSI GKGCMC 1 cut(s) 564
BseXI GCAGC 1 cut(s) 379
BshFI GGCC 1 cut(s) 249
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 1 cut(s) 183
BslFI GGGAC 1 cut(s) 508
BslI CCNNNNNNNGG 3 cut(s) 233, 358, 490
BsmAI GTCTC 1 cut(s) 247
BsmFI GGGAC 1 cut(s) 508
BsnI GGCC 1 cut(s) 249
BsoBI CYCGRG 1 cut(s) 563
Bsp1286I GDGCHC 1 cut(s) 564
Bsp143I GATC 4 cut(s) 109, 345, 425, 533
Bsp19I CCATGG 1 cut(s) 523
BspACI CCGC 1 cut(s) 491
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 3 cut(s) 21, 151, 367
BspHI TCATGA 1 cut(s) 55
BspMAI CTGCAG 1 cut(s) 217
BspPI GGATC 2 cut(s) 420, 541
BsrI ACTGG 3 cut(s) 9, 419, 547
BssECI CCNNGG 2 cut(s) 159, 523
BssMI GATC 4 cut(s) 109, 345, 425, 533
BssNI GRCGYC 1 cut(s) 87
BssT1I CCWWGG 2 cut(s) 159, 523
Bst6I CTCTTC 2 cut(s) 56, 62
BstACI GRCGYC 1 cut(s) 87
BstDEI CTNAG 3 cut(s) 29, 138, 375
BstDSI CCRYGG 1 cut(s) 523
BstF5I GGATG 3 cut(s) 22, 227, 348
BstKTI GATC 4 cut(s) 112, 348, 428, 536
BstMAI GTCTC 1 cut(s) 247
BstMBI GATC 4 cut(s) 109, 345, 425, 533
BstSFI CTRYAG 1 cut(s) 213
BstSLI GKGCMC 1 cut(s) 564
BstV1I GCAGC 1 cut(s) 379
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 425
BstXI CCANNNNNNTGG 1 cut(s) 530
BstYI RGATCY 1 cut(s) 425
BsuRI GGCC 1 cut(s) 249
BtgI CCRYGG 1 cut(s) 523
BtsCI GGATG 3 cut(s) 22, 227, 348
BtsIMutI CAGTG 2 cut(s) 16, 540
CciI TCATGA 1 cut(s) 55
Cfr13I GGNCC 2 cut(s) 247, 545
CseI GACGC 2 cut(s) 95, 110
Csp6I GTAC 2 cut(s) 329, 379
CviAII CATG 5 cut(s) 56, 326, 385, 394, 524
CviQI GTAC 2 cut(s) 329, 379
DdeI CTNAG 3 cut(s) 29, 138, 375
DpnI GATC 4 cut(s) 111, 347, 427, 535
DpnII GATC 4 cut(s) 109, 345, 425, 533
Eam1104I CTCTTC 2 cut(s) 56, 62
EarI CTCTTC 2 cut(s) 56, 62
Eco130I CCWWGG 2 cut(s) 159, 523
Eco47I GGWCC 1 cut(s) 545
Eco88I CYCGRG 1 cut(s) 563
EcoRI GAATTC 1 cut(s) 578
EcoT14I CCWWGG 2 cut(s) 159, 523
ErhI CCWWGG 2 cut(s) 159, 523
FaeI CATG 5 cut(s) 59, 329, 388, 397, 527
FaiI YATR 8 cut(s) 57, 179, 272, 327, 386, 395, 440, 525
FaqI GGGAC 1 cut(s) 508
FatI CATG 5 cut(s) 55, 325, 384, 393, 523
FauI CCCGC 1 cut(s) 484
FbaI TGATCA 1 cut(s) 109
Fnu4HI GCNGC 1 cut(s) 368
FokI GGATG 3 cut(s) 29, 214, 335
Fsp4HI GCNGC 1 cut(s) 368
FspBI CTAG 1 cut(s) 126
GluI GCNGC 1 cut(s) 368
HaeIII GGCC 1 cut(s) 249
HapII CCGG 1 cut(s) 183
HgaI GACGC 2 cut(s) 95, 110
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 5 cut(s) 59, 329, 388, 397, 527
HindIII AAGCTT 2 cut(s) 47, 75
HinfI GANTC 5 cut(s) 5, 25, 146, 209, 218
HpaII CCGG 1 cut(s) 183
HphI GGTGA 4 cut(s) 95, 141, 216, 313
Hpy188I TCNGA 4 cut(s) 30, 376, 425, 434
Hpy188III TCNNGA 4 cut(s) 56, 295, 352, 478
Hpy99I CGWCG 1 cut(s) 593
HpyAV CCTTC 2 cut(s) 100, 292
HpyCH4IV ACGT 1 cut(s) 469
HpyCH4V TGCA 1 cut(s) 215
HpyF3I CTNAG 3 cut(s) 29, 138, 375
HpySE526I ACGT 1 cut(s) 469
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 5 cut(s) 59, 329, 388, 397, 527
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 4 cut(s) 109, 345, 425, 533
LpnPI CCDG 7 cut(s) 22, 66, 196, 263, 365, 432, 528
Lsp1109I GCAGC 1 cut(s) 379
MaeI CTAG 1 cut(s) 126
MaeII ACGT 1 cut(s) 469
MaeIII GTNAC 2 cut(s) 83, 465
MalI GATC 4 cut(s) 111, 347, 427, 535
MboI GATC 4 cut(s) 109, 345, 425, 533
MfeI CAATTG 1 cut(s) 257
MflI RGATCY 1 cut(s) 425
MhlI GDGCHC 1 cut(s) 564
MluCI AATT 2 cut(s) 257, 578
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 2 cut(s) 123, 525
MnlI CCTC 6 cut(s) 7, 57, 131, 238, 428, 558
MseI TTAA 1 cut(s) 444
MslI CAYNNNNRTG 1 cut(s) 528
MspI CCGG 1 cut(s) 183
MunI CAATTG 1 cut(s) 257
NcoI CCATGG 1 cut(s) 523
NdeII GATC 4 cut(s) 109, 345, 425, 533
NlaIII CATG 5 cut(s) 59, 329, 388, 397, 527
NmeAIII GCCGAG 1 cut(s) 317
NmuCI GTSAC 1 cut(s) 83
PagI TCATGA 1 cut(s) 55
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PfeI GAWTC 4 cut(s) 25, 146, 209, 218
PkrI GCNGC 1 cut(s) 369
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PspPI GGNCC 2 cut(s) 247, 545
PstI CTGCAG 1 cut(s) 217
PsuI RGATCY 1 cut(s) 425
RsaI GTAC 2 cut(s) 330, 380
RsaNI GTAC 2 cut(s) 329, 379
RseI CAYNNNNRTG 1 cut(s) 528
SaqAI TTAA 1 cut(s) 444
SatI GCNGC 1 cut(s) 368
Sau3AI GATC 4 cut(s) 109, 345, 425, 533
Sau96I GGNCC 2 cut(s) 247, 545
ScaI AGTACT 1 cut(s) 380
SchI GAGTC 1 cut(s) 14
SduI GDGCHC 1 cut(s) 564
SfcI CTRYAG 1 cut(s) 213
SinI GGWCC 1 cut(s) 545
SmiMI CAYNNNNRTG 1 cut(s) 528
Sse9I AATT 2 cut(s) 257, 578
SsiI CCGC 1 cut(s) 491
SspMI CTAG 1 cut(s) 126
StyI CCWWGG 2 cut(s) 159, 523
TaiI ACGT 1 cut(s) 472
TaqI TCGA 1 cut(s) 588
TasI AATT 2 cut(s) 257, 578
TatI WGTACW 2 cut(s) 328, 378
TfiI GAWTC 4 cut(s) 25, 146, 209, 218
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TscAI CASTG 2 cut(s) 16, 547
TseFI GTSAC 1 cut(s) 83
TseI GCWGC 1 cut(s) 367
Tsp45I GTSAC 1 cut(s) 83
TspDTI ATGAA 3 cut(s) 44, 82, 382
TspRI CASTG 2 cut(s) 16, 547
VpaK11BI GGWCC 1 cut(s) 545
XapI RAATTY 1 cut(s) 578
XcmI CCANNNNNNNNNTGG 2 cut(s) 16, 521
XspI CTAG 1 cut(s) 126
ZrmI AGTACT 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.