MD16G1121100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014595
Physical Location & Seq
Reverse (-)
8711044 .. 8712399
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1121100.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGATTCGAAAGATGAGAACTTGGAGCTGATAAATGAGGCAATCAAGAGGCTCTTAGAGGAGAGGAAAAACAGTCAATCCTCCGCCGGCGAAGGCGTTTCCCAAGGTGGTGATGATGATGATGATGATGATGATAAAGATGAGGACCGCCTCCTCCTCCACCGATTGCTCTCTCAGTTGGAATCGTTAAAAGGCAATGGCGAGATTCGGAACTACGAAACTTCAACCAAAACGGGAGAGGAAACGTCCTCTACAAACGGCGAATTGAAGCCGGAAAATGAGAGTGGCGGCAAAGCAGATGGCGGTGGGGCTGAAATCGATACGGAAAAAATTGTGAGAGAAGTAAACAAGGTGAAGAAACAGAACAGCATCACTCACTGGCTGCTTTCTATCATGATTGTGCTCACTGTCGCCTGGCAAGTCTCCGAGGCGACTTTCCTTTGGAAACTCAACGAGGGATTCAGACACCCCTTTCGATGCATCGGAGGGATGCTGACGGGGAACGGGAAGGACTCGGAAGAACGCCACCGGAGTGAGTCTCCTTCTCTTCCTTCACTCCAAATACCTGAGCTCCCCTTGCCAGATATTAGTCTAAGTGAAAAGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

203

Amino Acids

22.46

Weight (kDa)

4.74

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014595)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27300
fragaria_vesca FvH4_4g23570
malus_domestica MD16G1121100.v1.1
prunus_persica Prupe.1G224100_v2.0.a1
pyrus_communis pycom13g10540 pycom16g10370
rosa_chinensis RchiOBHm_Chr4g0429971
rosa_laevigata RLG00000007016
rosa_multiflora Rmu_co8323629.1_g000001 Rmu_ssc0000150.1_g000037
rosa_roxburghii Rroxscaffold_5G00371800
rosa_rugosa Rorug04G0239800
rosa_samantha Rh4AG296200 Rh4BG302700 Rh4CG318200 Rh4DG300000
rosa_wichuraiana Rw4G025660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 84, 148, 288, 303
AgsI TTSAA 2 cut(s) 225, 268
AjnI CCWGG 1 cut(s) 413
AleI CACNNNNGTG 1 cut(s) 531
AluBI AGCT 2 cut(s) 28, 571
AluI AGCT 2 cut(s) 28, 571
Alw21I GWGCWC 2 cut(s) 405, 573
Alw26I GTCTC 2 cut(s) 427, 543
ApeKI GCWGC 1 cut(s) 382
ArsI GACNNNNNNTTYG 2 cut(s) 456, 488
AspS9I GGNCC 1 cut(s) 145
AsuHPI GGTGA 2 cut(s) 122, 364
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 1 cut(s) 145
BanII GRGCYC 1 cut(s) 573
Bbv12I GWGCWC 2 cut(s) 405, 573
BbvI GCAGC 1 cut(s) 369
BccI CCATC 1 cut(s) 293
BceAI ACGGC 1 cut(s) 274
BciT130I CCWGG 1 cut(s) 415
BcoDI GTCTC 2 cut(s) 427, 543
BisI GCNGC 2 cut(s) 289, 383
BlsI GCNGC 2 cut(s) 290, 384
Bme1390I CCNGG 1 cut(s) 415
Bme18I GGWCC 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 145
BmrFI CCNGG 1 cut(s) 415
BmsI GCATC 4 cut(s) 378, 467, 480, 489
BplI GAGNNNNNCTC 2 cut(s) 523, 555
Bpu10I CCTNAGC 1 cut(s) 567
Bpu14I TTCGAA 1 cut(s) 8
Bsa29I ATCGAT 1 cut(s) 318
BsaJI CCNNGG 2 cut(s) 103, 426
BsaWI WCCGGW 1 cut(s) 528
BsaXI ACNNNNNCTCC 2 cut(s) 555, 585
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 383
Bse3DI GCAATG 1 cut(s) 202
BseBI CCWGG 1 cut(s) 415
BseCI ATCGAT 1 cut(s) 318
BseDI CCNNGG 2 cut(s) 103, 426
BseGI GGATG 1 cut(s) 495
BseMI GCAATG 1 cut(s) 202
BseMII CTCAG 2 cut(s) 188, 558
BseNI ACTGG 1 cut(s) 383
BseRI GAGGAG 3 cut(s) 74, 143, 146
BseXI GCAGC 1 cut(s) 369
BshVI ATCGAT 1 cut(s) 318
BsiHKAI GWGCWC 2 cut(s) 405, 573
BsiSI CCGG 3 cut(s) 87, 272, 529
BsmAI GTCTC 2 cut(s) 427, 543
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 2 cut(s) 405, 573
BspACI CCGC 4 cut(s) 84, 148, 288, 303
BspCNI CTCAG 2 cut(s) 187, 559
BspDI ATCGAT 1 cut(s) 318
BspHI TCATGA 1 cut(s) 393
BspT104I TTCGAA 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 202
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 1 cut(s) 383
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 2 cut(s) 103, 426
BssT1I CCWWGG 1 cut(s) 103
Bst2UI CCWGG 1 cut(s) 415
Bst4CI ACNGT 2 cut(s) 74, 409
Bst6I CTCTTC 1 cut(s) 552
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 1 cut(s) 88
BstDEI CTNAG 4 cut(s) 55, 174, 567, 593
BstF5I GGATG 1 cut(s) 495
BstMAI GTCTC 2 cut(s) 427, 543
BstMWI GCNNNNNNNGC 1 cut(s) 577
BstNI CCWGG 1 cut(s) 415
BstSCI CCNGG 1 cut(s) 413
BstV1I GCAGC 1 cut(s) 369
Bsu15I ATCGAT 1 cut(s) 318
BsuTUI ATCGAT 1 cut(s) 318
BtsCI GGATG 1 cut(s) 495
BtsIMutI CAGTG 2 cut(s) 376, 405
Cac8I GCNNGC 1 cut(s) 88
CciI TCATGA 1 cut(s) 393
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 145
ClaI ATCGAT 1 cut(s) 318
CviAII CATG 1 cut(s) 394
CviJI RGCY 6 cut(s) 28, 52, 271, 311, 382, 571
CviKI_1 RGCY 6 cut(s) 28, 52, 271, 311, 382, 571
DdeI CTNAG 4 cut(s) 55, 174, 567, 593
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
EciI GGCGGA 1 cut(s) 73
Ecl136II GAGCTC 1 cut(s) 571
Eco130I CCWWGG 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 573
Eco47I GGWCC 1 cut(s) 145
Eco53kI GAGCTC 1 cut(s) 571
EcoICRI GAGCTC 1 cut(s) 571
EcoRII CCWGG 1 cut(s) 413
EcoT14I CCWWGG 1 cut(s) 103
EcoT22I ATGCAT 1 cut(s) 482
EcoT38I GRGCYC 1 cut(s) 573
ErhI CCWWGG 1 cut(s) 103
FaeI CATG 1 cut(s) 397
FaiI YATR 1 cut(s) 395
FalI AAGNNNNNCTT 2 cut(s) 38, 70
FatI CATG 1 cut(s) 393
Fnu4HI GCNGC 2 cut(s) 289, 383
FokI GGATG 1 cut(s) 502
FriOI GRGCYC 1 cut(s) 573
Fsp4HI GCNGC 2 cut(s) 289, 383
GluI GCNGC 2 cut(s) 289, 383
HapII CCGG 3 cut(s) 87, 272, 529
Hin1II CATG 1 cut(s) 397
HinfI GANTC 6 cut(s) 5, 182, 205, 459, 512, 536
HpaII CCGG 3 cut(s) 87, 272, 529
HphI GGTGA 2 cut(s) 122, 364
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 5 cut(s) 210, 427, 464, 485, 517
Hpy188III TCNNGA 2 cut(s) 46, 394
Hpy8I GTNNAC 1 cut(s) 346
HpyAV CCTTC 4 cut(s) 86, 502, 552, 561
HpyCH4III ACNGT 2 cut(s) 74, 409
HpyCH4IV ACGT 1 cut(s) 245
HpyCH4V TGCA 1 cut(s) 480
HpyF10VI GCNNNNNNNGC 1 cut(s) 577
HpyF3I CTNAG 4 cut(s) 55, 174, 567, 593
HpySE526I ACGT 1 cut(s) 245
Hsp92II CATG 1 cut(s) 397
KroI GCCGGC 1 cut(s) 86
KroNI GCCGGC 1 cut(s) 88
LmnI GCTCC 2 cut(s) 25, 576
LpnPI CCDG 8 cut(s) 100, 285, 364, 400, 427, 542, 579, 594
Lsp1109I GCAGC 1 cut(s) 369
LweI GCATC 4 cut(s) 378, 467, 480, 489
MaeII ACGT 1 cut(s) 245
MboII GAAGA 3 cut(s) 367, 530, 539
MhlI GDGCHC 2 cut(s) 405, 573
MluCI AATT 2 cut(s) 263, 330
MlyI GAGTC 2 cut(s) 506, 545
MmeI TCCRAC 1 cut(s) 159
Mph1103I ATGCAT 1 cut(s) 482
MreI CGCCGGCG 1 cut(s) 86
MroNI GCCGGC 1 cut(s) 86
MseI TTAA 1 cut(s) 188
MslI CAYNNNNRTG 2 cut(s) 398, 531
MspI CCGG 3 cut(s) 87, 272, 529
MspR9I CCNGG 1 cut(s) 415
MvaI CCWGG 1 cut(s) 415
MwoI GCNNNNNNNGC 1 cut(s) 577
NaeI GCCGGC 1 cut(s) 88
NgoMIV GCCGGC 1 cut(s) 86
NlaIII CATG 1 cut(s) 397
NsiI ATGCAT 1 cut(s) 482
NspV TTCGAA 1 cut(s) 8
OliI CACNNNNGTG 1 cut(s) 531
PagI TCATGA 1 cut(s) 393
PdiI GCCGGC 1 cut(s) 88
PfeI GAWTC 4 cut(s) 5, 182, 205, 459
PkrI GCNGC 2 cut(s) 290, 384
PleI GAGTC 2 cut(s) 506, 544
PpsI GAGTC 2 cut(s) 506, 544
Psp124BI GAGCTC 1 cut(s) 573
Psp6I CCWGG 1 cut(s) 413
PspGI CCWGG 1 cut(s) 413
PspPI GGNCC 1 cut(s) 145
RseI CAYNNNNRTG 2 cut(s) 398, 531
SacI GAGCTC 1 cut(s) 573
SaqAI TTAA 1 cut(s) 188
SatI GCNGC 2 cut(s) 289, 383
Sau96I GGNCC 1 cut(s) 145
SchI GAGTC 2 cut(s) 506, 545
ScrFI CCNGG 1 cut(s) 415
SduI GDGCHC 2 cut(s) 405, 573
SetI ASST 6 cut(s) 30, 109, 248, 354, 568, 573
SfaNI GCATC 4 cut(s) 378, 467, 480, 489
SfuI TTCGAA 1 cut(s) 8
SgrAI CRCCGGYG 1 cut(s) 86
SinI GGWCC 1 cut(s) 145
SmiMI CAYNNNNRTG 2 cut(s) 398, 531
Sse9I AATT 2 cut(s) 263, 330
SsiI CCGC 4 cut(s) 84, 148, 288, 303
SstI GAGCTC 1 cut(s) 573
StyD4I CCNGG 1 cut(s) 413
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 2 cut(s) 74, 409
TaiI ACGT 1 cut(s) 248
TaqI TCGA 3 cut(s) 8, 318, 475
TasI AATT 2 cut(s) 263, 330
TauI GCSGC 1 cut(s) 291
TfiI GAWTC 4 cut(s) 5, 182, 205, 459
Tru1I TTAA 1 cut(s) 188
Tru9I TTAA 1 cut(s) 188
TscAI CASTG 2 cut(s) 383, 412
TseI GCWGC 1 cut(s) 382
TspGWI ACGGA 1 cut(s) 338
TspRI CASTG 2 cut(s) 383, 412
VpaK11BI GGWCC 1 cut(s) 145
Zsp2I ATGCAT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.