AT1G27750

SPOC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008266
Physical Location & Seq
Forward (+)
9657173 .. 9661994
4822 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27750.1

Sequence Viewer

Length: 3228 bp
ATGGCTTGCGCCGAGCAGCCTATCAAAAAGCGTCGTCTGTATGAGTCAATCCCTGAATCTCACCCTCCGCCACCACCGCAACTAGAATCCCAATCCCCTTCTACGGTGGTAAGCTCGTTCCCGGCTCCGGTGACACCGTCGCCTCCATCTCAGGAGGAAATTCAAACGAGAAGTAGGAACAGAGAAGAGATTCGAAGAGTGCATGACTGCTATAAGCGTCTTAAATCTTGTGTTGCTCAGAGAGATGGTGGCGGCCGCTCTGCGAATCTTGAGCAAGCGTATCGCTCCCTCATCTCCGCTTCCAGAGGTTGTACTAGCGTTAAGCGCCTTGTGGCTGATCTCGTTCCTCGATATGCATTGTACTGTCCTACGGCTATTGGAGATGCAGTGCAAGCTGTTATTGACATGCATAACTTCAGCTTGGAAGCTTTAAAGAGAGGACAAGATGCTGATGGTGTTGCTTTTCAAACTGCTAAAGCCTGCATATTTGGTCTAGTTGACCTATGTTCTGCTGCTTTATCGAAGAAAACGTCATCACCAGGGGCTCGAGATATCTGCTCTGCCGTGTTCAGAAATGTGTTAACCTTCTTTGTGCTTTCTTTTGAGGGGAAAAATATCTTCCAGATAGTTGATAAGAGCGATTTGAAGTTGCAAGATCCTGACGAAATTTTTTCTCAGCTGATGCAAAAACTTTCAGATGGAAACTCGTTACCTTTGATCAAATTATCCCAGTTTCGTGTTTTAGCTTTACTGAAAGTATTCTTCAACTTCCCTAAAAAATCAATTGCCACTTGTTTCGGATTCTTCAATTCTTCATCCACGGAGGATGTAGCAACGGGGAGGTATCTCATTACTCATATGACAGAAAAGATTAATGATATTGATGCCGCTAGTATTGAACCTGAAGTAGACGAGAATTCTGGTCAGACAGGTTCTAACAATATAGAGGCTACTGGTAAGAATGCAGAAGGGTTGAATGGTGTACAAGAAGCATCAAACAGTCTAACGAGCTGCTTGTTAGAAATGGTTATTAGGAAGAGTTCCTCCATTGGTAGGTGGGCCTTCTTTCAGTATAAGAAAATATGCAGCCTGTCATCCTTCGTGGATATATCCAGTGCTGTTACTTCTCTAGAGGGAATCTTTGGATTTGTTGGAAAAAATATCAAGTTAGAAGATTGTCAAATGGACAGTGACGAGGATGATTATGGAAAATTTTCAGTTTCACATGTGAAACCTCACAGTTCTGCAGACAATGATGTCCGCTCTAGTGCTGGATCGGTTTATGATGCTGGAGGTTCACGATCTATGGATTTTGAGACTGTTGACCAGAGAGACTTGTCATGTGGTAGATCTTCAGTCCCAAGGGGATTATTAAACCAGCATACACCATCTCCTTCTGCAAGAGGGCCTTCAGATTTAAGAAGTAACTCTACGGATCGTAGGAACAATTTTGTTCTTGCAGGATCACCTGTTTATCAGGCAGTACCCCATGGCCCTTCATCAGGGCAAATTGCTTGGTATTTGGATGGGGACCCCACTGCTTTTGACATTTTTCCGGCTTCGGGGCAGTTGTGGTTAGGCTATTTAGGACCAGATGAAACAGAAGGTCATCTAAGGTTTCAGCTTGACAGGTACGGCCCTGTAGACCGTTTCTTTTTTGATCCAGTGAAAGGATTTGCTCTAGCAGAATATAGAAGCATAATTGATGCAATCAGAGCTCGAGAATATCTGCGAGCGCAATTTCCTTGGCGCATTAAATTTATGGATATAGGAGTGGGTGCAAGGGGATCTTTGAATGGAGTAGCATATGGTTATTGTACTCATCTGTATATTGGAGGCATCAGTAGCCAGTGGGAGAGGGATGAAATTGTCCATGAGTCGCGGCAAGCCCTTTATAAGGGGCCTCGCATGGTGACTGACCTGTACTATGAACATGCCCTTCTGATGGAGTTTGATACACCTGATGATGCTGCAATTGTAATGGCCCATCTCAGGTTTTTTCGGGGAGAAAAGAGTAAATTTCATTTGGCATCAATCAATCGTCCTTTGCCTCATGAAGATGGAGGAAGTCATCCAGAGAGGCACCTTCAAGTACCTCCCTCATCAAAACAGGATAGTGGTTCAGGAGAATATGTTTCCCCCTTAATGAGTACGGATAACCACTGTAATTCTGTACCTCCTGGAGCTACCTTTCAGCAAAACTGGCCTGCAGCTGGTAGCACTCTTGTGAATTCTGCACAAGGAGTCTCAGGAACTCCTCCATGTGTGCCCATACCAGCTCCGGGCCAGCCTGCAGTGCCTGCTACACCGACATCTCAAATCCCTCCATCTCCGTTTGTTCAACAACCCATTTACCCTCCTCCAAACAGCTCTTGGGATACAAGATCCCTAATCTCTCCAAGCGGTGATGCTGTAGCAACATCATCTCAGATGCAAGGACCTCCAGCGCAACAGGTTTCAGGTCCCTTCATGCCGCCTCCTGTGCATCCAGTCTCTCAGCCACAAGGACCTCAAGTTCAGCAATTTGATCAATTGTACCCACCTCCGCCTTTAGGACATTCATTACCTTCTGTACTCCAGCCACCACTACAACCTCAATCCCAGCCACCGGAACCACCGCCTGAAATGATGCCGCCTCCGCCTCAAGCTCTACCACCTCCTTTGCCTCACTCACACCCACCTCTGGTTCCTCCTCCTCCTTTTTCTCCACTGTTGTCCCCACGCTTACCTCCAATGGTCACCCAGTTGTGTGGATCAGAGGCTTCCAAACAGAACATAGGGCACCAATGGCAGGGTGCTTTGTCCAAAAGTGGAGTTCATTACTCCACCATCATTGCACAGAGATTGGAATCAGACATTTGCAAATATATCATTGGATCTCCCGAGCCTGTGCAATGGCCAGTTAAACTGGATATGACTAAGCGCACGGATATGAAGAACGTAAAAGCTACTTTTGCGAATACACAACCACACAAAAGGGAGGTTTGTCAGTTAATTCCTGCTGCTTTCAGCGATCGCAAAGGGCTCCAAGATTTCATAACATACTTGAAGCAGAGAGATTGTGCAGGAGTAATAAAGATCCCTGCATCAAGTCCCATGTGGGCAAGGCATCTCTTCATTCTTCCACACTCGCAAGAGACATGTTCCTTGCTTTCTGTTTCCCCATCTTCCTCGGAATGCTTGATCGGTTTGGTTTTGCCAAAGGATACAAATGCTGAGGCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 SPOC

Protein Analysis

1075

Amino Acids

117.45

Weight (kDa)

6.4

Isoelectric Point (pI)

59.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPOC PF07744 922 - 1067 3.2e-25 SPOC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1896
AasI GACNNNNNNGTC 1 cut(s) 1256
AccB1I GGYRCC 2 cut(s) 2082, 2781
AccBSI CCGCTC 2 cut(s) 258, 1263
AccI GTMKAC 2 cut(s) 909, 1644
AccII CGCG 1 cut(s) 1882
AcoI YGGCCR 2 cut(s) 253, 2897
AcsI RAATTY 7 cut(s) 159, 666, 916, 1211, 1757, 2018, 2230
AcuI CTGAAG 4 cut(s) 400, 924, 1338, 1395
AflIII ACRYGT 2 cut(s) 1225, 3140
AjnI CCWGG 2 cut(s) 538, 2179
AleI CACNNNNGTG 1 cut(s) 2225
AloI GAACNNNNNNTCC 2 cut(s) 2499, 2531
Alw21I GWGCWC 1 cut(s) 1720
Alw26I GTCTC 5 cut(s) 1310, 1326, 2251, 2497, 3131
AlwNI CAGNNNCTG 1 cut(s) 2300
Ama87I CYCGRG 3 cut(s) 546, 1719, 2882
ApeKI GCWGC 7 cut(s) 16, 512, 1011, 1086, 1970, 2210, 3002
ApoI RAATTY 7 cut(s) 159, 666, 916, 1211, 1757, 2018, 2230
ArsI GACNNNNNNTTYG 2 cut(s) 515, 547
AseI ATTAAT 1 cut(s) 873
AsiSI GCGATCGC 1 cut(s) 3016
Asp700I GAANNNNTTC 3 cut(s) 189, 758, 1213
AspLEI GCGC 6 cut(s) 11, 327, 1738, 1752, 2449, 2925
AsuC2I CCSGG 2 cut(s) 122, 2283
AsuHPI GGTGA 7 cut(s) 53, 142, 528, 1458, 1924, 2417, 2731
AsuII TTCGAA 1 cut(s) 193
AvaI CYCGRG 3 cut(s) 546, 1719, 2882
AvaII GGWCC 5 cut(s) 1531, 1589, 2438, 2462, 2507
BaeGI GKGCMC 2 cut(s) 2271, 2784
BalI TGGCCA 1 cut(s) 2899
BanI GGYRCC 2 cut(s) 2082, 2781
BanII GRGCYC 3 cut(s) 547, 1720, 3027
BarI GAAGNNNNNNTAC 2 cut(s) 1414, 1446
Bbv12I GWGCWC 1 cut(s) 1720
BbvCI CCTCAGC 1 cut(s) 3216
BbvI GCAGC 7 cut(s) 28, 499, 998, 1098, 1957, 2222, 2989
BceAI ACGGC 3 cut(s) 387, 548, 1651
BcgI CGANNNNNNTGC 4 cut(s) 263, 297, 537, 571
BciT130I CCWGG 2 cut(s) 540, 2181
BciVI GTATCC 2 cut(s) 2371, 3199
BclI TGATCA 2 cut(s) 717, 2527
BcnI CCSGG 2 cut(s) 122, 2283
BcoDI GTCTC 5 cut(s) 1310, 1326, 2251, 2497, 3131
BfaI CTAG 7 cut(s) 83, 315, 494, 891, 1130, 1266, 1682
BfmI CTRYAG 5 cut(s) 1245, 1641, 2208, 2292, 2412
BfoI RGCGCY 1 cut(s) 328
BfuI GTATCC 2 cut(s) 2371, 3199
BglII AGATCT 1 cut(s) 1349
Bme1390I CCNGG 4 cut(s) 122, 540, 2181, 2283
Bme18I GGWCC 5 cut(s) 1531, 1589, 2438, 2462, 2507
BmeT110I CYCGRG 3 cut(s) 546, 1719, 2882
BmrFI CCNGG 4 cut(s) 122, 540, 2181, 2283
BmrI ACTGGG 2 cut(s) 724, 2737
BmuI ACTGGG 2 cut(s) 724, 2737
BpmI CTGGAG 4 cut(s) 1311, 2202, 2427, 2561
Bpu10I CCTNAGC 1 cut(s) 3216
Bpu14I TTCGAA 1 cut(s) 193
BpuEI CTTGAG 3 cut(s) 290, 2496, 2628
BpuMI CCSGG 2 cut(s) 122, 2283
BsaBI GATNNNNATC 1 cut(s) 2848
BsaJI CCNNGG 6 cut(s) 539, 819, 1361, 1489, 1745, 3171
BsaWI WCCGGW 2 cut(s) 127, 2608
BsaXI ACNNNNNCTCC 6 cut(s) 372, 402, 1375, 1405, 2118, 2148
Bse3DI GCAATG 2 cut(s) 2832, 2900
Bse8I GATNNNNATC 1 cut(s) 2848
BseBI CCWGG 2 cut(s) 540, 2181
BseDI CCNNGG 6 cut(s) 539, 819, 1361, 1489, 1745, 3171
BseGI GGATG 8 cut(s) 815, 832, 1094, 1204, 1531, 1867, 2071, 2485
BseJI GATNNNNATC 1 cut(s) 2848
BseMI GCAATG 2 cut(s) 2832, 2900
BseMII CTCAG 8 cut(s) 164, 251, 689, 2005, 2262, 2441, 2510, 3207
BseRI GAGGAG 4 cut(s) 2247, 2349, 2682, 2685
BseSI GKGCMC 2 cut(s) 2271, 2784
BseX3I CGGCCG 1 cut(s) 253
BseXI GCAGC 7 cut(s) 28, 499, 998, 1098, 1957, 2222, 2989
BseYI CCCAGC 1 cut(s) 2601
BsgI GTGCAG 2 cut(s) 2220, 3084
Bsh1236I CGCG 1 cut(s) 1882
Bsh1285I CGRYCG 2 cut(s) 256, 3016
BshNI GGYRCC 2 cut(s) 2082, 2781
BsiEI CGRYCG 2 cut(s) 256, 3016
BsiHKAI GWGCWC 1 cut(s) 1720
BsiHKCI CYCGRG 3 cut(s) 546, 1719, 2882
BsiSI CCGG 5 cut(s) 122, 128, 1556, 2282, 2609
BslFI GGGAC 5 cut(s) 1343, 1544, 2448, 2701, 3078
BsmAI GTCTC 5 cut(s) 1310, 1326, 2251, 2497, 3131
BsmFI GGGAC 5 cut(s) 1343, 1544, 2448, 2701, 3078
BsmI GAATGC 2 cut(s) 967, 3182
BsoBI CYCGRG 3 cut(s) 546, 1719, 2882
Bsp119I TTCGAA 1 cut(s) 193
Bsp1286I GDGCHC 5 cut(s) 547, 1720, 2271, 2784, 3027
Bsp1407I TGTACA 1 cut(s) 982
Bsp19I CCATGG 1 cut(s) 1489
BspCNI CTCAG 8 cut(s) 163, 250, 688, 2004, 2261, 2440, 2509, 3208
BspFNI CGCG 1 cut(s) 1882
BspHI TCATGA 2 cut(s) 2053, 3224
BspMAI CTGCAG 3 cut(s) 1249, 2212, 2296
BspT104I TTCGAA 1 cut(s) 193
BspT107I GGYRCC 2 cut(s) 2082, 2781
BsrBI CCGCTC 2 cut(s) 258, 1263
BsrDI GCAATG 2 cut(s) 2832, 2900
BsrGI TGTACA 1 cut(s) 982
BssECI CCNNGG 6 cut(s) 539, 819, 1361, 1489, 1745, 3171
BssT1I CCWWGG 3 cut(s) 1361, 1489, 1745
Bst2UI CCWGG 2 cut(s) 540, 2181
Bst6I CTCTTC 4 cut(s) 180, 190, 1031, 3119
BstAPI GCANNNNNTGC 1 cut(s) 2300
BstAUI TGTACA 1 cut(s) 982
BstBI TTCGAA 1 cut(s) 193
BstDSI CCRYGG 2 cut(s) 819, 1489
BstEII GGTNACC 1 cut(s) 2737
BstENI CCTNNNNNAGG 2 cut(s) 1500, 1895
BstF5I GGATG 8 cut(s) 815, 832, 1094, 1204, 1531, 1867, 2071, 2485
BstFNI CGCG 1 cut(s) 1882
BstH2I RGCGCY 1 cut(s) 328
BstHHI GCGC 6 cut(s) 11, 327, 1738, 1752, 2449, 2925
BstMAI GTCTC 5 cut(s) 1310, 1326, 2251, 2497, 3131
BstMCI CGRYCG 2 cut(s) 256, 3016
BstNI CCWGG 2 cut(s) 540, 2181
BstNSI RCATGY 4 cut(s) 409, 1229, 1937, 3144
BstPI GGTNACC 1 cut(s) 2737
BstSCI CCNGG 4 cut(s) 120, 538, 2179, 2281
BstSFI CTRYAG 5 cut(s) 1245, 1641, 2208, 2292, 2412
BstSLI GKGCMC 2 cut(s) 2271, 2784
BstUI CGCG 1 cut(s) 1882
BstV1I GCAGC 7 cut(s) 28, 499, 998, 1098, 1957, 2222, 2989
BstX2I RGATCY 6 cut(s) 655, 1349, 1787, 2384, 2876, 3078
BstXI CCANNNNNNTGG 1 cut(s) 2750
BstYI RGATCY 6 cut(s) 655, 1349, 1787, 2384, 2876, 3078
BstZI CGGCCG 1 cut(s) 253
BsuI GTATCC 2 cut(s) 2371, 3199
BtgI CCRYGG 2 cut(s) 819, 1489
BtsCI GGATG 8 cut(s) 815, 832, 1094, 1204, 1531, 1867, 2071, 2485
BtsI GCAGTG 3 cut(s) 393, 1536, 2301
BtsIMutI CAGTG 9 cut(s) 393, 1120, 1195, 1536, 1671, 1856, 2161, 2301, 2708
CaiI CAGNNNCTG 1 cut(s) 2300
CciI TCATGA 2 cut(s) 2053, 3224
CciNI GCGGCCGC 1 cut(s) 253
CfoI GCGC 6 cut(s) 11, 327, 1738, 1752, 2449, 2925
CseI GACGC 2 cut(s) 20, 206
CspCI CAANNNNNGTGG 6 cut(s) 1525, 1560, 2643, 2678, 2815, 2850
DraI TTTAAA 1 cut(s) 432
DrdI GACNNNNNNGTC 1 cut(s) 1256
DseDI GACNNNNNNGTC 1 cut(s) 1256
EaeI YGGCCR 2 cut(s) 253, 2897
EagI CGGCCG 1 cut(s) 253
Eam1104I CTCTTC 4 cut(s) 180, 190, 1031, 3119
EarI CTCTTC 4 cut(s) 180, 190, 1031, 3119
EciI GGCGGA 3 cut(s) 57, 2535, 2628
Ecl136II GAGCTC 1 cut(s) 1718
EclXI CGGCCG 1 cut(s) 253
Eco130I CCWWGG 3 cut(s) 1361, 1489, 1745
Eco147I AGGCCT 1 cut(s) 3221
Eco24I GRGCYC 3 cut(s) 547, 1720, 3027
Eco32I GATATC 1 cut(s) 553
Eco47I GGWCC 5 cut(s) 1531, 1589, 2438, 2462, 2507
Eco52I CGGCCG 1 cut(s) 253
Eco53kI GAGCTC 1 cut(s) 1718
Eco57I CTGAAG 4 cut(s) 400, 924, 1338, 1395
Eco88I CYCGRG 3 cut(s) 546, 1719, 2882
Eco91I GGTNACC 1 cut(s) 2737
EcoICRI GAGCTC 1 cut(s) 1718
EcoNI CCTNNNNNAGG 2 cut(s) 1500, 1895
EcoO109I RGGNCCY 6 cut(s) 1406, 1531, 1901, 2438, 2462, 2507
EcoO65I GGTNACC 1 cut(s) 2737
EcoRI GAATTC 2 cut(s) 916, 2230
EcoRII CCWGG 2 cut(s) 538, 2179
EcoRV GATATC 1 cut(s) 553
EcoT14I CCWWGG 3 cut(s) 1361, 1489, 1745
EcoT22I ATGCAT 2 cut(s) 358, 411
EcoT38I GRGCYC 3 cut(s) 547, 1720, 3027
ErhI CCWWGG 3 cut(s) 1361, 1489, 1745
FalI AAGNNNNNCTT 4 cut(s) 1393, 1425, 1774, 1806
FaqI GGGAC 5 cut(s) 1343, 1544, 2448, 2701, 3078
FauNDI CATATG 2 cut(s) 858, 1807
FbaI TGATCA 2 cut(s) 717, 2527
FblI GTMKAC 2 cut(s) 909, 1644
FokI GGATG 8 cut(s) 802, 839, 1081, 1211, 1538, 1874, 2058, 2472
FriOI GRGCYC 3 cut(s) 547, 1720, 3027
FspBI CTAG 7 cut(s) 83, 315, 494, 891, 1130, 1266, 1682
GlaI GCGC 6 cut(s) 10, 326, 1737, 1751, 2448, 2924
GsaI CCCAGC 1 cut(s) 2605
GsuI CTGGAG 4 cut(s) 1311, 2202, 2427, 2561
HaeII RGCGCY 1 cut(s) 328
HapII CCGG 5 cut(s) 122, 128, 1556, 2282, 2609
HgaI GACGC 2 cut(s) 20, 206
HhaI GCGC 6 cut(s) 11, 327, 1738, 1752, 2449, 2925
Hin6I GCGC 6 cut(s) 9, 325, 1736, 1750, 2447, 2923
HinP1I GCGC 6 cut(s) 9, 325, 1736, 1750, 2447, 2923
HincII GTYRAC 3 cut(s) 499, 582, 1324
HindII GTYRAC 3 cut(s) 499, 582, 1324
HindIII AAGCTT 1 cut(s) 426
HpaI GTTAAC 1 cut(s) 582
HpaII CCGG 5 cut(s) 122, 128, 1556, 2282, 2609
HphI GGTGA 7 cut(s) 53, 142, 528, 1458, 1924, 2417, 2731
Hpy166II GTNNAC 7 cut(s) 499, 582, 910, 983, 1298, 1324, 1645
Hpy8I GTNNAC 7 cut(s) 499, 582, 910, 983, 1298, 1324, 1645
Hpy99I CGWCG 2 cut(s) 36, 142
HpyCH4IV ACGT 2 cut(s) 530, 2940
HpySE526I ACGT 2 cut(s) 530, 2940
HspAI GCGC 6 cut(s) 9, 325, 1736, 1750, 2447, 2923
KflI GGGWCCC 1 cut(s) 1531
Ksp22I TGATCA 2 cut(s) 717, 2527
KspAI GTTAAC 1 cut(s) 582
LmnI GCTCC 5 cut(s) 130, 290, 2183, 2284, 3030
Lsp1109I GCAGC 7 cut(s) 28, 499, 998, 1098, 1957, 2222, 2989
MaeI CTAG 7 cut(s) 83, 315, 494, 891, 1130, 1266, 1682
MaeII ACGT 2 cut(s) 530, 2940
MaeIII GTNAC 7 cut(s) 130, 708, 1120, 1190, 1424, 1912, 2737
MbiI CCGCTC 2 cut(s) 258, 1263
MfeI CAATTG 3 cut(s) 783, 1974, 2531
MflI RGATCY 6 cut(s) 655, 1349, 1787, 2384, 2876, 3078
MhlI GDGCHC 5 cut(s) 547, 1720, 2271, 2784, 3027
MlsI TGGCCA 1 cut(s) 2899
MluNI TGGCCA 1 cut(s) 2899
MlyI GAGTC 3 cut(s) 53, 1886, 2253
MmeI TCCRAC 1 cut(s) 1132
Mox20I TGGCCA 1 cut(s) 2899
Mph1103I ATGCAT 2 cut(s) 358, 411
MroXI GAANNNNTTC 3 cut(s) 189, 758, 1213
MscI TGGCCA 1 cut(s) 2899
MslI CAYNNNNRTG 3 cut(s) 2058, 2225, 2930
Msp20I TGGCCA 1 cut(s) 2899
MspA1I CMGCKG 2 cut(s) 679, 2213
MspI CCGG 5 cut(s) 122, 128, 1556, 2282, 2609
MspR9I CCNGG 4 cut(s) 122, 540, 2181, 2283
MunI CAATTG 3 cut(s) 783, 1974, 2531
Mva1269I GAATGC 2 cut(s) 967, 3182
MvaI CCWGG 2 cut(s) 540, 2181
MvnI CGCG 1 cut(s) 1882
NciI CCSGG 2 cut(s) 122, 2283
NcoI CCATGG 1 cut(s) 1489
NdeI CATATG 2 cut(s) 858, 1807
NmeAIII GCCGAG 1 cut(s) 37
NmuCI GTSAC 4 cut(s) 130, 1190, 1912, 2737
NotI GCGGCCGC 1 cut(s) 253
NsiI ATGCAT 2 cut(s) 358, 411
NspI RCATGY 4 cut(s) 409, 1229, 1937, 3144
NspV TTCGAA 1 cut(s) 193
OliI CACNNNNGTG 1 cut(s) 2225
PaeR7I CTCGAG 2 cut(s) 546, 1719
PagI TCATGA 2 cut(s) 2053, 3224
PceI AGGCCT 1 cut(s) 3221
PciI ACATGT 2 cut(s) 1225, 3140
PcsI WCGNNNNNNNCGW 1 cut(s) 527
PctI GAATGC 2 cut(s) 967, 3182
PdmI GAANNNNTTC 3 cut(s) 189, 758, 1213
PfeI GAWTC 7 cut(s) 56, 86, 190, 265, 801, 1137, 2849
PflFI GACNNNGTC 1 cut(s) 136
PfoI TCCNGGA 1 cut(s) 2179
Ple19I CGATCG 1 cut(s) 3016
PleI GAGTC 3 cut(s) 52, 1885, 2252
PpsI GAGTC 3 cut(s) 52, 1885, 2252
PpuMI RGGWCCY 4 cut(s) 1531, 2438, 2462, 2507
PscI ACATGT 2 cut(s) 1225, 3140
PshBI ATTAAT 1 cut(s) 873
PsiI TTATAA 1 cut(s) 1896
Psp124BI GAGCTC 1 cut(s) 1720
Psp5II RGGWCCY 4 cut(s) 1531, 2438, 2462, 2507
Psp6I CCWGG 2 cut(s) 538, 2179
PspEI GGTNACC 1 cut(s) 2737
PspFI CCCAGC 1 cut(s) 2601
PspGI CCWGG 2 cut(s) 538, 2179
PspPPI RGGWCCY 4 cut(s) 1531, 2438, 2462, 2507
PstI CTGCAG 3 cut(s) 1249, 2212, 2296
PstNI CAGNNNCTG 1 cut(s) 2300
PsuI RGATCY 6 cut(s) 655, 1349, 1787, 2384, 2876, 3078
PsyI GACNNNGTC 1 cut(s) 136
PvuI CGATCG 1 cut(s) 3016
PvuII CAGCTG 2 cut(s) 679, 2213
RgaI GCGATCGC 1 cut(s) 3016
RseI CAYNNNNRTG 3 cut(s) 2058, 2225, 2930
SacI GAGCTC 1 cut(s) 1720
SchI GAGTC 3 cut(s) 53, 1886, 2253
ScrFI CCNGG 4 cut(s) 122, 540, 2181, 2283
SduI GDGCHC 5 cut(s) 547, 1720, 2271, 2784, 3027
SfaAI GCGATCGC 1 cut(s) 3016
SfcI CTRYAG 5 cut(s) 1245, 1641, 2208, 2292, 2412
Sfr274I CTCGAG 2 cut(s) 546, 1719
SfuI TTCGAA 1 cut(s) 193
SgfI GCGATCGC 1 cut(s) 3016
SinI GGWCC 5 cut(s) 1531, 1589, 2438, 2462, 2507
SlaI CTCGAG 2 cut(s) 546, 1719
SmiMI CAYNNNNRTG 3 cut(s) 2058, 2225, 2930
SmlI CTYRAG 5 cut(s) 269, 546, 1719, 2511, 2643
SmoI CTYRAG 5 cut(s) 269, 546, 1719, 2511, 2643
SseBI AGGCCT 1 cut(s) 3221
SspMI CTAG 7 cut(s) 83, 315, 494, 891, 1130, 1266, 1682
SstI GAGCTC 1 cut(s) 1720
StuI AGGCCT 1 cut(s) 3221
StyD4I CCNGG 4 cut(s) 120, 538, 2179, 2281
StyI CCWWGG 3 cut(s) 1361, 1489, 1745
TaiI ACGT 2 cut(s) 533, 2943
TaqI TCGA 5 cut(s) 193, 349, 521, 547, 1720
TatI WGTACW 6 cut(s) 311, 360, 982, 1817, 1923, 2572
TauI GCSGC 6 cut(s) 255, 258, 890, 1885, 2476, 2635
TfiI GAWTC 7 cut(s) 56, 86, 190, 265, 801, 1137, 2849
TscAI CASTG 9 cut(s) 393, 1120, 1195, 1543, 1671, 1856, 2168, 2301, 2715
TseFI GTSAC 4 cut(s) 130, 1190, 1912, 2737
TseI GCWGC 7 cut(s) 16, 512, 1011, 1086, 1970, 2210, 3002
Tsp45I GTSAC 4 cut(s) 130, 1190, 1912, 2737
TspGWI ACGGA 5 cut(s) 836, 1448, 2168, 2322, 2942
TspRI CASTG 9 cut(s) 393, 1120, 1195, 1543, 1671, 1856, 2168, 2301, 2715
Tth111I GACNNNGTC 1 cut(s) 136
VpaK11BI GGWCC 5 cut(s) 1531, 1589, 2438, 2462, 2507
VspI ATTAAT 1 cut(s) 873
XagI CCTNNNNNAGG 2 cut(s) 1500, 1895
XapI RAATTY 7 cut(s) 159, 666, 916, 1211, 1757, 2018, 2230
XbaI TCTAGA 1 cut(s) 1129
XceI RCATGY 4 cut(s) 409, 1229, 1937, 3144
XcmI CCANNNNNNNNNTGG 1 cut(s) 2370
XhoI CTCGAG 2 cut(s) 546, 1719
XmiI GTMKAC 2 cut(s) 909, 1644
XmnI GAANNNNTTC 3 cut(s) 189, 758, 1213
XspI CTAG 7 cut(s) 83, 315, 494, 891, 1130, 1266, 1682
Zsp2I ATGCAT 2 cut(s) 358, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.