Rh6DG485600

SPOC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008266
Physical Location & Seq
Forward (+)
64777275 .. 64783317
6043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG485600.1

Sequence Viewer

Length: 3117 bp
ATGCATAACTGGAGTGTGGGGCTGATAAATAGAGGAGAGGATGCTAATGGCTTTGCATTCCACACTGCGAAATCCTGTATTCTTGGTCTATCGGATATTTGTTGCACTGCTTCCTCGGTGGCGTCAACATCCTCTGTCATTCGCGGTATTTGCTCCGGCGTTTTTCAGAATGTGCTTACCTTCTTTATATCGTCCTTTGAGGGGAAGGATGTCTTTCTGATTGTTGGTAAAGAGACTGTGAGAATGCAAGGTTCTGCTGATATCTTTCTTCAGCTAAAGGAGAAGTTTTCTGATGGAAATGAATCTTCAGTGACTAAGTTATCCCAGTTACGTGCTCTTTGTTTACTGTGGATTTTCTTTTGTTATCCTAAAGACTCACTTGCAGCCTGCTTTGAACTCTTGAAATCCACCTCATCAGAGGGAGTTCTTAAAGGGCAGTATTTTCTAAGTCAAATAACAAGCAAACTCGATAAGGATGGCGGGCATCATTTGAGCAAAACTAGTGATGAATCTAAAACAAGTACAACTTTTGTTGAAACAAGCAACGGAGGCAATGATGTTAGCTCTGAGCAGCTAGCATCAGATGGAAACAACGTCTTTGAAGATGCGATTTCAGTTTCAAATATCTGCTTGTTAGGACTGGTTCTTAGTAAAGATCCATTATTGCAAAGTTGGATGCTCTCAAAATATAAGAAGTTATGTAGATTGCCATCTATTTCAGATTTTAAATCTGCTTTGGAGGGTGTCTTTAAATCTTTTATAGATGTAATGGACGTAGAAGGTAATCGAGCAGACAGTGATGAGGATGATTCTGATCCATCGAGATTTATCAATCGACCATATCTGGTGTCTAGGTTCTCTAACCTACATGAAACTGGCAGTGATGTTTATTCTGATAGAGTGTCAGGTCAACATATGAAAGCTCGTAGCTCTGTAATGCCTCTCGAAGCTAATAGTCACTCAATGGGTTCCAATCAGGATAGTGGCGGTGCAAGGCCCATGGACTTTGAGATTAGGGAGCATGGAGATATGTCACATGGCAGGTCTTCCATGCCCAGGGACGTAGTGAACCACCAGATGCCTTCACCTGTCACAAGGTCTCCATTAAGCTTCAGGAGTAATTCGTTTGATGGGAGAAACCACTTTCATATTGAGAAGAACAAAGATACAAATTTGGATTTTGGTTCACCTCCCATGAGATCTTCAAGTGGAGGTGCAAACAGTTCTTTTGAATCTCCCAAATGTCATCCTGTGTCATATGTGTCCCCCAAGACTCAAATTGTTTGGTATTTTGATGGGGATATTGCAGCCATGGACATTTTCTCAGCTTCTAAACAGCTCTGGCTGGGCTTTTTTGGTTCTGATGTATCTGAAGCCCATGTAAGGTTTCAGTTAGAGAGGTTTGGTGCCATGGAACAATTTTTTTTCTTTCCTCATAAAGGATTTGCCGTAGTTGAGTATAGAAACATTCTTGATGCCATAAAGGCTCGTGAATATACGCGCAGGCATTTTCCTTGGTGCATAAAGTTTATGGATACAGGACTGGGAACTAGAGGTGCTATGAATGGTGTTGCAGTTGGTTCTAGTTGTCATGTTTATGTTGGAAATGTTTCAAGTCAATGGGCCAAGGATGAGATTCTTCACGAATCAAGGAAGGTGCTTCGCAAGACTCCATACTCGGTCACTGATTTCAGCAATGAAGGTGCGGTACTGATGGAATTTGATACTCCCGAAGAAGCTGCTGCTGTGATGGCACATCTCAGACAGCATCGCAAGGAAAGGAGTAACCACAGGGCACCATATGGTGCCGGACCACTTAATGTAGTATCTCACATTGATGGTGCCAGATCTTTACCTACCCCTACCCATGTTGACATCAGAAGCAACCAGGGAAACATGTCTAATACTGCTGCTACCCCATTTGCAGTAAAGCATGAGAGCCACTCAATGGAGCTTGTATCCCCTAGAGTAAAATCTGAGAATCAGGGGAATTCAGTACAAAGTGGATATATGTTTCAGTCAAACAGAGCTGTCACTGGCTCTACAGAGATGCTTGAAGCTGGCACCAGAAGAGTTGATGGTTATGACAGTAACTTAGCTGTGAATTCTACACAAGGAGGTGGTCACGTTGCTTCTCATGCCAATGAACCTAGGTGGTTGTATTCAAAACCTGGAATGGAACTGCATTCTGCACCAGGGAGCATTCCATGCATTCCTGTACCAACACAGATACCCTCAGCTCCACCTCCTCCACAAATTTCCTCATCTCCATTTATGCGACCTGTTTACCTCCCTCCAAATAGTTCTTGGGATCCACGGGGTTCAAGTCACAATCGTCCTTTGAACCCAATCTCACCAGGTGTAGTCCCCAACAATTTTCATGGTAATGCTAATGTACCTCCCTTCATTCCTGCTTCTGTGACTCCACTTGCACAAATACAGGGAGCTCCAGTGCAGCAATTTGACCGTATGTTTTCAGTACCCATAGCACCACCACCATTATCATCTCTGCCACCTCCTCTACCTGAAATGCCACCTCCGTTGTCGCCACCCCCACCGCCTTTGCCTCAATCACAGCCACCTTTTGTTCCCCCTCCACCTTGTTCCCCCTCCCCTCCTGTGGCTGTTTCAGAACCATCTGTTGTGGAAACTTCTGGACAACCCTCACAATATCAGTGGCAGGGAACATTGTGTAAGAGTGGTGTTCATTACAGTACAGTTAATGCATATAGAGTGGATTCTGATATTTGCAAATATTCCAATGCCACTTCTGAACCTATAGAATGGCCTCTTAAGTTAGACATGACCAAGCGTACAGATTTTCGGCATGTGAAGTCAACATTTACTAGCACCCCACCACATAAGAGGGAAGTGTGTCGATTGATCCCATCTTCTGCAGCCGATCAGAAAGGGTTTCAGGATTTCTTGTCATACTTGAAGCAGAGGGACTGCTCTGGAGTGATCAAGATCCCAGCGGTGAATTCCATGTGGGCCAGACTTCTCTTCATACTTCCATACTCGGAAGAGACTTGCTCTATGCTCTCTATTGCGCCTTGTCAACCTGATTGCCTCATTGCTTTAATATTACCTAAAGAAACGAATTTTGAGTGGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 SPOC

Protein Analysis

1038

Amino Acids

114.07

Weight (kDa)

6.56

Isoelectric Point (pI)

51.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPOC PF07744 885 - 1030 9.7e-22 SPOC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1032
AccB1I GGYRCC 5 cut(s) 1406, 1795, 1805, 1841, 2063
AccB7I CCANNNNNTGG 1 cut(s) 1948
AccII CGCG 2 cut(s) 144, 1501
AciI CCGC 6 cut(s) 144, 480, 987, 1706, 2558, 2975
AclWI GGATC 6 cut(s) 650, 809, 2306, 2319, 2878, 2962
AcsI RAATTY 7 cut(s) 1171, 1718, 1990, 2104, 2256, 2980, 3100
AcuI CTGAAG 4 cut(s) 254, 291, 1096, 1392
AcyI GRCGYC 1 cut(s) 122
AdeI CACNNNGTG 1 cut(s) 2360
AfaI GTAC 8 cut(s) 523, 1710, 1998, 2220, 2397, 2481, 2716, 2815
AfiI CCNNNNNNNGG 6 cut(s) 201, 1056, 1383, 1439, 1948, 2620
AflII CTTAAG 1 cut(s) 2792
AflIII ACRYGT 1 cut(s) 1896
AhlI ACTAGT 1 cut(s) 500
AjnI CCWGG 5 cut(s) 1055, 1887, 2170, 2194, 2356
Alw21I GWGCWC 2 cut(s) 337, 2449
Alw26I GTCTC 3 cut(s) 227, 1104, 3020
AlwI GGATC 6 cut(s) 650, 809, 2306, 2319, 2878, 2962
AoxI GGCC 4 cut(s) 995, 1623, 2786, 2991
ApeKI GCWGC 8 cut(s) 383, 571, 1307, 1739, 1742, 1910, 2455, 2897
ApoI RAATTY 7 cut(s) 1171, 1718, 1990, 2104, 2256, 2980, 3100
Asp700I GAANNNNTTC 1 cut(s) 1609
AspA2I CCTAGG 1 cut(s) 2150
AspLEI GCGC 2 cut(s) 1503, 3052
AspS9I GGNCC 4 cut(s) 996, 1623, 1811, 2991
AsuHPI GGTGA 4 cut(s) 1077, 1179, 2346, 2989
AsuNHI GCTAGC 1 cut(s) 574
AvaII GGWCC 1 cut(s) 1811
AvrII CCTAGG 1 cut(s) 2150
BaeGI GKGCMC 1 cut(s) 1798
BamHI GGATCC 1 cut(s) 2311
BanI GGYRCC 5 cut(s) 1406, 1795, 1805, 1841, 2063
BanII GRGCYC 1 cut(s) 2449
BarI GAAGNNNNNNTAC 2 cut(s) 1692, 1724
BauI CACGAG 1 cut(s) 1488
BbsI GAAGAC 1 cut(s) 1038
Bbv12I GWGCWC 2 cut(s) 337, 2449
BbvCI CCTCAGC 1 cut(s) 2236
BbvI GCAGC 8 cut(s) 395, 583, 1319, 1726, 1729, 1897, 2467, 2909
BceAI ACGGC 1 cut(s) 1433
BcgI CGANNNNNNTGC 2 cut(s) 1721, 1755
BciT130I CCWGG 5 cut(s) 1057, 1889, 2172, 2196, 2358
BciVI GTATCC 2 cut(s) 1528, 1969
BclI TGATCA 1 cut(s) 2961
BcoDI GTCTC 3 cut(s) 227, 1104, 3020
BcuI ACTAGT 1 cut(s) 500
BfaI CTAG 8 cut(s) 501, 575, 852, 1551, 1584, 1965, 2151, 2847
BfmI CTRYAG 3 cut(s) 2043, 2778, 2895
BfrI CTTAAG 1 cut(s) 2792
BfuAI ACCTGC 1 cut(s) 1032
BfuI GTATCC 2 cut(s) 1528, 1969
BglI GCCNNNNNGGC 1 cut(s) 1484
BglII AGATCT 2 cut(s) 1199, 1847
BisI GCNGC 8 cut(s) 384, 572, 1308, 1740, 1743, 1911, 2456, 2898
BlnI CCTAGG 1 cut(s) 2150
BlsI GCNGC 8 cut(s) 385, 573, 1309, 1741, 1744, 1912, 2457, 2899
Bme1390I CCNGG 5 cut(s) 1057, 1889, 2172, 2196, 2358
Bme18I GGWCC 1 cut(s) 1811
BmgT120I GGNCC 4 cut(s) 996, 1623, 1811, 2991
BmiI GGNNCC 7 cut(s) 970, 1408, 1797, 1807, 1843, 2065, 2313
BmrFI CCNGG 5 cut(s) 1057, 1889, 2172, 2196, 2358
BmrI ACTGGG 2 cut(s) 319, 1553
BmsI GCATC 9 cut(s) 31, 493, 587, 595, 666, 1068, 1465, 1777, 2040
BmtI GCTAGC 1 cut(s) 578
BmuI ACTGGG 2 cut(s) 319, 1553
BpiI GAAGAC 1 cut(s) 1038
BplI GAGNNNNNCTC 4 cut(s) 1928, 1960, 3017, 3049
BpmI CTGGAG 3 cut(s) 31, 2433, 2976
Bpu10I CCTNAGC 1 cut(s) 2236
BsaAI YACGTR 1 cut(s) 332
BsaBI GATNNNNATC 3 cut(s) 709, 813, 2966
BsaHI GRCGYC 1 cut(s) 122
BsaI GGTCTC 1 cut(s) 1104
BsaXI ACNNNNNCTCC 4 cut(s) 1084, 1114, 2436, 2466
Bsc4I CCNNNNNNNGG 6 cut(s) 201, 1056, 1383, 1439, 1948, 2620
Bse1I ACTGG 7 cut(s) 14, 325, 645, 880, 1548, 2041, 2450
Bse3DI GCAATG 3 cut(s) 559, 1702, 3072
Bse8I GATNNNNATC 3 cut(s) 709, 813, 2966
BseBI CCWGG 5 cut(s) 1057, 1889, 2172, 2196, 2358
BseGI GGATG 8 cut(s) 46, 128, 214, 481, 681, 811, 1246, 1636
BseJI GATNNNNATC 3 cut(s) 709, 813, 2966
BseLI CCNNNNNNNGG 6 cut(s) 201, 1056, 1383, 1439, 1948, 2620
BseMI GCAATG 3 cut(s) 559, 1702, 3072
BseMII CTCAG 5 cut(s) 558, 1338, 1774, 1968, 2250
BseNI ACTGG 7 cut(s) 14, 325, 645, 880, 1548, 2041, 2450
BseRI GAGGAG 3 cut(s) 48, 2238, 2508
BseSI GKGCMC 1 cut(s) 1798
BseXI GCAGC 8 cut(s) 395, 583, 1319, 1726, 1729, 1897, 2467, 2909
BseYI CCCAGC 2 cut(s) 1345, 2971
BsgI GTGCAG 2 cut(s) 2175, 2474
Bsh1236I CGCG 2 cut(s) 144, 1501
BshFI GGCC 4 cut(s) 997, 1625, 2788, 2993
BshNI GGYRCC 5 cut(s) 1406, 1795, 1805, 1841, 2063
BsiHKAI GWGCWC 2 cut(s) 337, 2449
BsiSI CCGG 2 cut(s) 156, 1809
BslFI GGGAC 4 cut(s) 1073, 1249, 2351, 2960
BslI CCNNNNNNNGG 6 cut(s) 201, 1056, 1383, 1439, 1948, 2620
BsmAI GTCTC 3 cut(s) 227, 1104, 3020
BsmFI GGGAC 4 cut(s) 1073, 1249, 2351, 2960
BsmI GAATGC 5 cut(s) 56, 249, 2185, 2202, 2211
BsnI GGCC 4 cut(s) 997, 1625, 2788, 2993
Bso31I GGTCTC 1 cut(s) 1104
Bsp1286I GDGCHC 3 cut(s) 337, 1798, 2449
Bsp143I GATC 9 cut(s) 655, 814, 1199, 1847, 2311, 2883, 2902, 2961, 2967
Bsp19I CCATGG 3 cut(s) 999, 1311, 1410
BspACI CCGC 6 cut(s) 144, 480, 987, 1706, 2558, 2975
BspANI GGCC 4 cut(s) 997, 1625, 2788, 2993
BspCNI CTCAG 5 cut(s) 559, 1337, 1773, 1969, 2249
BspFNI CGCG 2 cut(s) 144, 1501
BspLI GGNNCC 7 cut(s) 970, 1408, 1797, 1807, 1843, 2065, 2313
BspMAI CTGCAG 1 cut(s) 2899
BspMI ACCTGC 1 cut(s) 1032
BspOI GCTAGC 1 cut(s) 578
BspPI GGATC 6 cut(s) 650, 809, 2306, 2319, 2878, 2962
BspT107I GGYRCC 5 cut(s) 1406, 1795, 1805, 1841, 2063
BspTI CTTAAG 1 cut(s) 2792
BspTNI GGTCTC 1 cut(s) 1104
BsrDI GCAATG 3 cut(s) 559, 1702, 3072
BsrI ACTGG 7 cut(s) 14, 325, 645, 880, 1548, 2041, 2450
BssMI GATC 9 cut(s) 655, 814, 1199, 1847, 2311, 2883, 2902, 2961, 2967
BssNI GRCGYC 1 cut(s) 122
BssSI CACGAG 1 cut(s) 1488
BssT1I CCWWGG 6 cut(s) 999, 1311, 1410, 1514, 1626, 2150
Bst2BI CACGAG 1 cut(s) 1488
Bst2UI CCWGG 5 cut(s) 1057, 1889, 2172, 2196, 2358
Bst4CI ACNGT 8 cut(s) 238, 348, 797, 1223, 2090, 2468, 2714, 2719
Bst6I CTCTTC 3 cut(s) 2065, 3008, 3018
BstACI GRCGYC 1 cut(s) 122
BstAFI CTTAAG 1 cut(s) 2792
BstAPI GCANNNNNTGC 1 cut(s) 2208
BstBAI YACGTR 1 cut(s) 332
BstC8I GCNNGC 5 cut(s) 388, 482, 576, 1505, 2062
BstDEI CTNAG 9 cut(s) 315, 446, 567, 647, 1324, 1760, 1977, 2095, 2236
BstDSI CCRYGG 4 cut(s) 999, 1311, 1410, 2315
BstENI CCTNNNNNAGG 1 cut(s) 1437
BstF5I GGATG 8 cut(s) 46, 128, 214, 481, 681, 811, 1246, 1636
BstFNI CGCG 2 cut(s) 144, 1501
BstHHI GCGC 2 cut(s) 1503, 3052
BstKTI GATC 9 cut(s) 658, 817, 1202, 1850, 2314, 2886, 2905, 2964, 2970
BstMAI GTCTC 3 cut(s) 227, 1104, 3020
BstMBI GATC 9 cut(s) 655, 814, 1199, 1847, 2311, 2883, 2902, 2961, 2967
BstMWI GCNNNNNNNGC 6 cut(s) 150, 549, 1484, 1751, 2138, 2208
BstNI CCWGG 5 cut(s) 1057, 1889, 2172, 2196, 2358
BstNSI RCATGY 2 cut(s) 1900, 2831
BstSCI CCNGG 5 cut(s) 1055, 1887, 2170, 2194, 2356
BstSFI CTRYAG 3 cut(s) 2043, 2778, 2895
BstSLI GKGCMC 1 cut(s) 1798
BstUI CGCG 2 cut(s) 144, 1501
BstV1I GCAGC 8 cut(s) 395, 583, 1319, 1726, 1729, 1897, 2467, 2909
BstV2I GAAGAC 1 cut(s) 1038
BstX2I RGATCY 5 cut(s) 655, 1199, 1847, 2311, 2967
BstYI RGATCY 5 cut(s) 655, 1199, 1847, 2311, 2967
BsuI GTATCC 2 cut(s) 1528, 1969
BsuRI GGCC 4 cut(s) 997, 1625, 2788, 2993
BtgI CCRYGG 4 cut(s) 999, 1311, 1410, 2315
BtgZI GCGATG 1 cut(s) 1754
BtsCI GGATG 8 cut(s) 46, 128, 214, 481, 681, 811, 1246, 1636
BtsI GCAGTG 3 cut(s) 63, 105, 886
BtsIMutI CAGTG 9 cut(s) 63, 105, 315, 802, 886, 1683, 2034, 2457, 2681
BveI ACCTGC 1 cut(s) 1032
Cac8I GCNNGC 5 cut(s) 388, 482, 576, 1505, 2062
CfoI GCGC 2 cut(s) 1503, 3052
Cfr13I GGNCC 4 cut(s) 996, 1623, 1811, 2991
CseI GACGC 1 cut(s) 111
CsiI ACCWGGT 1 cut(s) 2356
Csp6I GTAC 8 cut(s) 522, 1709, 1997, 2219, 2396, 2480, 2715, 2814
CspCI CAANNNNNGTGG 6 cut(s) 2523, 2544, 2558, 2579, 2657, 2692
CviQI GTAC 8 cut(s) 522, 1709, 1997, 2219, 2396, 2480, 2715, 2814
DdeI CTNAG 9 cut(s) 315, 446, 567, 647, 1324, 1760, 1977, 2095, 2236
DpnI GATC 9 cut(s) 657, 816, 1201, 1849, 2313, 2885, 2904, 2963, 2969
DpnII GATC 9 cut(s) 655, 814, 1199, 1847, 2311, 2883, 2902, 2961, 2967
DraI TTTAAA 2 cut(s) 727, 751
DraIII CACNNNGTG 1 cut(s) 2360
Eam1104I CTCTTC 3 cut(s) 2065, 3008, 3018
EarI CTCTTC 3 cut(s) 2065, 3008, 3018
Ecl136II GAGCTC 1 cut(s) 2447
Eco130I CCWWGG 6 cut(s) 999, 1311, 1410, 1514, 1626, 2150
Eco24I GRGCYC 1 cut(s) 2449
Eco31I GGTCTC 1 cut(s) 1104
Eco32I GATATC 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 1811
Eco53kI GAGCTC 1 cut(s) 2447
Eco57I CTGAAG 4 cut(s) 254, 291, 1096, 1392
EcoICRI GAGCTC 1 cut(s) 2447
EcoNI CCTNNNNNAGG 1 cut(s) 1437
EcoRI GAATTC 3 cut(s) 1990, 2104, 2980
EcoRII CCWGG 5 cut(s) 1055, 1887, 2170, 2194, 2356
EcoRV GATATC 1 cut(s) 262
EcoT14I CCWWGG 6 cut(s) 999, 1311, 1410, 1514, 1626, 2150
EcoT22I ATGCAT 3 cut(s) 6, 2213, 2728
EcoT38I GRGCYC 1 cut(s) 2449
ErhI CCWWGG 6 cut(s) 999, 1311, 1410, 1514, 1626, 2150
FalI AAGNNNNNCTT 6 cut(s) 197, 229, 363, 395, 511, 543
FaqI GGGAC 4 cut(s) 1073, 1249, 2351, 2960
FauI CCCGC 1 cut(s) 473
FauNDI CATATG 3 cut(s) 915, 1258, 1801
FbaI TGATCA 1 cut(s) 2961
Fnu4HI GCNGC 8 cut(s) 384, 572, 1308, 1740, 1743, 1911, 2456, 2898
FokI GGATG 8 cut(s) 53, 115, 221, 488, 688, 818, 1233, 1643
FriOI GRGCYC 1 cut(s) 2449
Fsp4HI GCNGC 8 cut(s) 384, 572, 1308, 1740, 1743, 1911, 2456, 2898
FspBI CTAG 8 cut(s) 501, 575, 852, 1551, 1584, 1965, 2151, 2847
GlaI GCGC 2 cut(s) 1502, 3051
GluI GCNGC 8 cut(s) 384, 572, 1308, 1740, 1743, 1911, 2456, 2898
GsaI CCCAGC 2 cut(s) 1349, 2975
GsuI CTGGAG 3 cut(s) 31, 2433, 2976
HaeIII GGCC 4 cut(s) 997, 1625, 2788, 2993
HapII CCGG 2 cut(s) 156, 1809
HgaI GACGC 1 cut(s) 111
HhaI GCGC 2 cut(s) 1503, 3052
Hin1I GRCGYC 1 cut(s) 122
Hin6I GCGC 2 cut(s) 1501, 3050
HinP1I GCGC 2 cut(s) 1501, 3050
HincII GTYRAC 5 cut(s) 126, 911, 1873, 2838, 3059
HindII GTYRAC 5 cut(s) 126, 911, 1873, 2838, 3059
HindIII AAGCTT 1 cut(s) 1108
HpaII CCGG 2 cut(s) 156, 1809
HphI GGTGA 4 cut(s) 1077, 1179, 2346, 2989
Hpy166II GTNNAC 9 cut(s) 126, 344, 911, 1069, 1187, 1873, 2287, 2838, 3059
Hpy8I GTNNAC 9 cut(s) 126, 344, 911, 1069, 1187, 1873, 2287, 2838, 3059
HpyAV CCTTC 7 cut(s) 190, 199, 773, 1092, 1648, 1694, 2413
HpyCH4III ACNGT 8 cut(s) 238, 348, 797, 1223, 2090, 2468, 2714, 2719
HpyCH4IV ACGT 5 cut(s) 331, 594, 774, 1062, 2127
HpyF10VI GCNNNNNNNGC 6 cut(s) 150, 549, 1484, 1751, 2138, 2208
HpyF3I CTNAG 9 cut(s) 315, 446, 567, 647, 1324, 1760, 1977, 2095, 2236
HpySE526I ACGT 5 cut(s) 331, 594, 774, 1062, 2127
Hsp92I GRCGYC 1 cut(s) 122
HspAI GCGC 2 cut(s) 1501, 3050
Ksp22I TGATCA 1 cut(s) 2961
Kzo9I GATC 9 cut(s) 655, 814, 1199, 1847, 2311, 2883, 2902, 2961, 2967
LmnI GCTCC 7 cut(s) 158, 1018, 1951, 2199, 2245, 2444, 2452
Lsp1109I GCAGC 8 cut(s) 395, 583, 1319, 1726, 1729, 1897, 2467, 2909
LweI GCATC 9 cut(s) 31, 493, 587, 595, 666, 1068, 1465, 1777, 2040
MabI ACCWGGT 1 cut(s) 2356
MaeI CTAG 8 cut(s) 501, 575, 852, 1551, 1584, 1965, 2151, 2847
MaeII ACGT 5 cut(s) 331, 594, 774, 1062, 2127
MalI GATC 9 cut(s) 657, 816, 1201, 1849, 2313, 2885, 2904, 2963, 2969
MboI GATC 9 cut(s) 655, 814, 1199, 1847, 2311, 2883, 2902, 2961, 2967
MflI RGATCY 5 cut(s) 655, 1199, 1847, 2311, 2967
MhlI GDGCHC 3 cut(s) 337, 1798, 2449
MlyI GAGTC 4 cut(s) 368, 1267, 1663, 2416
MmeI TCCRAC 2 cut(s) 653, 1582
Mph1103I ATGCAT 3 cut(s) 6, 2213, 2728
MroXI GAANNNNTTC 1 cut(s) 1609
MseI TTAA 8 cut(s) 429, 726, 750, 1106, 1818, 2721, 2793, 3080
MslI CAYNNNNRTG 4 cut(s) 1596, 1836, 2142, 2385
MspA1I CMGCKG 1 cut(s) 2975
MspCI CTTAAG 1 cut(s) 2792
MspI CCGG 2 cut(s) 156, 1809
MspR9I CCNGG 5 cut(s) 1057, 1889, 2172, 2196, 2358
Mva1269I GAATGC 5 cut(s) 56, 249, 2185, 2202, 2211
MvaI CCWGG 5 cut(s) 1057, 1889, 2172, 2196, 2358
MvnI CGCG 2 cut(s) 144, 1501
MwoI GCNNNNNNNGC 6 cut(s) 150, 549, 1484, 1751, 2138, 2208
NcoI CCATGG 3 cut(s) 999, 1311, 1410
NdeI CATATG 3 cut(s) 915, 1258, 1801
NdeII GATC 9 cut(s) 655, 814, 1199, 1847, 2311, 2883, 2902, 2961, 2967
NheI GCTAGC 1 cut(s) 574
NlaIV GGNNCC 7 cut(s) 970, 1408, 1797, 1807, 1843, 2065, 2313
NmuCI GTSAC 9 cut(s) 310, 956, 1032, 1090, 1681, 2032, 2123, 2327, 2419
NsiI ATGCAT 3 cut(s) 6, 2213, 2728
NspI RCATGY 2 cut(s) 1900, 2831
PasI CCCWGGG 1 cut(s) 1056
PciI ACATGT 1 cut(s) 1896
PctI GAATGC 5 cut(s) 56, 249, 2185, 2202, 2211
PdmI GAANNNNTTC 1 cut(s) 1609
PfeI GAWTC 8 cut(s) 302, 509, 809, 1232, 1636, 1646, 1981, 2738
PflMI CCANNNNNTGG 1 cut(s) 1948
PkrI GCNGC 8 cut(s) 385, 573, 1309, 1741, 1744, 1912, 2457, 2899
PleI GAGTC 4 cut(s) 368, 1267, 1663, 2416
PpsI GAGTC 4 cut(s) 368, 1267, 1663, 2416
Ppu21I YACGTR 1 cut(s) 332
PscI ACATGT 1 cut(s) 1896
Psp124BI GAGCTC 1 cut(s) 2449
Psp6I CCWGG 5 cut(s) 1055, 1887, 2170, 2194, 2356
PspFI CCCAGC 2 cut(s) 1345, 2971
PspGI CCWGG 5 cut(s) 1055, 1887, 2170, 2194, 2356
PspN4I GGNNCC 7 cut(s) 970, 1408, 1797, 1807, 1843, 2065, 2313
PspPI GGNCC 4 cut(s) 996, 1623, 1811, 2991
PstI CTGCAG 1 cut(s) 2899
PsuI RGATCY 5 cut(s) 655, 1199, 1847, 2311, 2967
RsaI GTAC 8 cut(s) 523, 1710, 1998, 2220, 2397, 2481, 2716, 2815
RsaNI GTAC 8 cut(s) 522, 1709, 1997, 2219, 2396, 2480, 2715, 2814
RseI CAYNNNNRTG 4 cut(s) 1596, 1836, 2142, 2385
SacI GAGCTC 1 cut(s) 2449
SaqAI TTAA 8 cut(s) 429, 726, 750, 1106, 1818, 2721, 2793, 3080
SatI GCNGC 8 cut(s) 384, 572, 1308, 1740, 1743, 1911, 2456, 2898
Sau3AI GATC 9 cut(s) 655, 814, 1199, 1847, 2311, 2883, 2902, 2961, 2967
Sau96I GGNCC 4 cut(s) 996, 1623, 1811, 2991
SchI GAGTC 4 cut(s) 368, 1267, 1663, 2416
ScrFI CCNGG 5 cut(s) 1057, 1889, 2172, 2196, 2358
SduI GDGCHC 3 cut(s) 337, 1798, 2449
SexAI ACCWGGT 1 cut(s) 2356
SfaNI GCATC 9 cut(s) 31, 493, 587, 595, 666, 1068, 1465, 1777, 2040
SfcI CTRYAG 3 cut(s) 2043, 2778, 2895
SinI GGWCC 1 cut(s) 1811
SmiMI CAYNNNNRTG 4 cut(s) 1596, 1836, 2142, 2385
SmlI CTYRAG 1 cut(s) 2792
SmoI CTYRAG 1 cut(s) 2792
SpeI ACTAGT 1 cut(s) 500
SsiI CCGC 6 cut(s) 144, 480, 987, 1706, 2558, 2975
SspI AATATT 2 cut(s) 2756, 3084
SspMI CTAG 8 cut(s) 501, 575, 852, 1551, 1584, 1965, 2151, 2847
SstI GAGCTC 1 cut(s) 2449
StyD4I CCNGG 5 cut(s) 1055, 1887, 2170, 2194, 2356
StyI CCWWGG 6 cut(s) 999, 1311, 1410, 1514, 1626, 2150
TaaI ACNGT 8 cut(s) 238, 348, 797, 1223, 2090, 2468, 2714, 2719
TaiI ACGT 5 cut(s) 334, 597, 777, 1065, 2130
TaqI TCGA 6 cut(s) 468, 787, 821, 835, 945, 2878
TaqII GACCGA 1 cut(s) 1669
TatI WGTACW 3 cut(s) 521, 1996, 2714
TfiI GAWTC 8 cut(s) 302, 509, 809, 1232, 1636, 1646, 1981, 2738
Tru1I TTAA 8 cut(s) 429, 726, 750, 1106, 1818, 2721, 2793, 3080
Tru9I TTAA 8 cut(s) 429, 726, 750, 1106, 1818, 2721, 2793, 3080
TscAI CASTG 9 cut(s) 70, 112, 315, 802, 886, 1690, 2041, 2457, 2681
TseFI GTSAC 9 cut(s) 310, 956, 1032, 1090, 1681, 2032, 2123, 2327, 2419
TseI GCWGC 8 cut(s) 383, 571, 1307, 1739, 1742, 1910, 2455, 2897
Tsp45I GTSAC 9 cut(s) 310, 956, 1032, 1090, 1681, 2032, 2123, 2327, 2419
TspGWI ACGGA 2 cut(s) 561, 2529
TspRI CASTG 9 cut(s) 70, 112, 315, 802, 886, 1690, 2041, 2457, 2681
Van91I CCANNNNNTGG 1 cut(s) 1948
Vha464I CTTAAG 1 cut(s) 2792
VpaK11BI GGWCC 1 cut(s) 1811
XagI CCTNNNNNAGG 1 cut(s) 1437
XapI RAATTY 7 cut(s) 1171, 1718, 1990, 2104, 2256, 2980, 3100
XceI RCATGY 2 cut(s) 1900, 2831
XcmI CCANNNNNNNNNTGG 1 cut(s) 2304
XmaJI CCTAGG 1 cut(s) 2150
XmnI GAANNNNTTC 1 cut(s) 1609
XspI CTAG 8 cut(s) 501, 575, 852, 1551, 1584, 1965, 2151, 2847
Zsp2I ATGCAT 3 cut(s) 6, 2213, 2728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.