Rh6BG494500

SPOC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0008266
Physical Location & Seq
Forward (+)
69620611 .. 69626071
5461 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG494500.1

Sequence Viewer

Length: 3519 bp
ATGGCCTCCGCAGAGCAGCCCCCGAAGAAGCGAAGGCTGCACGAAGCCGAAGCAGAGCCACCATCCTCATCGCCGCCACCATCTCCGCCACCACCGCCACAATCCCTAGCCCAACGGCCGAGCGCGGCAGCTCCAGAATCTCTCTCCCAGGACGAAATTCTCAGGAAACGTAGACACCGCGACGAGATTCGAGGCGTTTACGACTGCTATAAGCGGATTAAGTTCTGCCTCTCCAAGAAGGACTCTGCTCTCACCGCCGATCTTGAGCAAGCCTATCTCTCCCTCATCACGGCCTCCAGAGGTTGTACGAGTGTACAACGCATTGTGGCTGATCTCATTCCTAGATATGCTTCGAAATGCCCCACTGCTCTTGAAGCCGCGGCAAGAGTTGTTGTCAATATGCATAACTGGAGTGTGGGGCTGATAAATAGAGGAGAGGATACTAATGGCTTTGCATTCCAGACTGCGAAATCCTGTATTCTTGGTCTATCGGATATTTGTTGCACTGCTTCCTCGGTGGGGTCAACATCCTCTGTCATTCGCGGTATTTGCTCCGGCGTTTTTCAGAATGTGCTTACCTTCTTTATATCGTCTTTTGAGTGGAAGGATGTCTTTCTGATTGTTGGAAAAGAGACTGTGAGAATGCAAGGTTCTGCTGATATCTTTCTTGAGCTAAAGGAGAAGTTTTCTGATGGAAATGAATCTTCGGTGACTAAGTTATCCCAGTTACGTGCTCTTTGTTTACTGTGGATTTTCTTTTGTTATCCTAAAGACTCACTTGCAGCCTGCTTTGAACTCTTGAAATCCACCTCATCAGAGGGAGTTCTTAAAGGGCAGTATTTTCTAAGTCAAATAACAAGCAAACTCGATAAGGATGGCGGGCATCATTTGAGCAAAACTAGTGATGAATCTAAAACAAGTACAACTTTTGTTGAAACAAGCAACGGAGGCAATGATGTTAGCTCTGAGCAGCTAGCATCAGATGGAAACAACGTCTTTGAAGATGCGATTTCAGTTTCAAATATCTGCTTGTTAGGACTGGTTCTTAGTAAAGATCCATTATTGCAAAGTTGGATGCTCTCAAAATATAAGAAGTTATGTAGATTGCCATCTATTTCAGATTTTAAATCTGCTTTGGAGGGTGTCTTTAAATCTTTTATAGATGTAATGGACGTAGAAGGTAATCGAGCAGACAGTGATGAGGATGATTCTGATCCATCGAGATTTATCAATCGACCATATCTGGTGTCTAGGTTCTCTAACCTACATGAAACTGGCAGTGATGTTTATTCTGATAGAGTGTCAGGTCAACATATGAAAGCTCGTAGCTCTGTAATGCCTCTTGAAGCTAATAGTCACTCAACAGGTTCCAATCAGGATAGTGGCGGTGCAAGGCCCATGGACTTTGAGATTAGGGAGCATGGAGATATGTCACATGGCAGGTCTTCCATGCCCAGGGACGTAGTGAACCACCAGATGCCTTCACCTGTCACAAGGTCACCATTAAGCTTTAGGAGTAATTCGTTTGATGGGAGAAACCACTTTCATATTGAGAAGAACAAAGATGCAAATTTGGATTTTGGTTCACCTCCCATGAGATCTTCAAGTGGAGGTGCAAACAGTTCTTTTGAATCTCCCAAATGTCATCCTGTGTCGTCATATGTGTCCCCCAAGACTCAAATTGTTTGGTATTCTGACGGGGATATTGCAGCCATGGACATTTTCTCAGCTTCTAAACAGCTCTGGCTGGGCTTTTTTGGTTCTGATGTATCTGAAGCCCATGTAAGGTTTCAGTTAGAGAGGTTTGGTGCCATGGAACAATTTTTTTTCTTTCCTCATAAAGGATTTGCCGTAGTTGAGTATAGAAACATTCTTGATGCCATAAAGGCTCGTGAATATACGCGCAGGCATTTTCCTTGGTGCATAAAGTTTATGGATACAGGACTGGGAACTAGAGGTGCTATGAATGGTGTTGCAGTTGGTTCTAGTTGTCATGTTTATGTTGGAAATGTTTCAAGTCAATGGGCCAAGGATGAGATTCTTCACGAATCAAGGAAGGTGCTTCGCAAGACTCCATACTCGGTCACTGATTTCAGCAATGAAGGTGCGGTACTGATGGAATTTGATACTCCTGAAGAAGCTGCTGCTGTGATGGCACATCTCAGACAGCATCGCAAGGAAAGGAGTAACCACAGGGCACCATATGGTGCCGGACCACTTAATGTAGTATCTCACATTGATGGTGCCAGATCTTTACCTACCCCTACCCATGTTGACATCAGAAGCAACCAGGGAAACATGTCTAATACTGCTGCTACCCCATTTGCAGTAAAGCATGAGAGCCACTCAATGGAGCTTGTATCCCCTAGAGTAAAATCTGAGAATCAGGGGAATTCAGTACAAAGTGGATATATGTTTCAGTCAAACAGAGCTGTCACTGGCTCTACAGAGATGCTTGAAGCTGGCACCAGAAGAGTTGATGGTTATGACAGTAACTTAGCTGTGAATTCTACACAAGGAGGTGGTCACGTTGCTTCTCATGCCAATGAACCTAGGTGGTTGTATTCAAAACCTGGAATGGAGCTGCATTCTGCACCAGGGAGCATTCCATGCATTCCTGTACCAACACAGATACCCTCAGCTCCACCTCCTCCACAAATTTCCTCATCTCCATTTATGCGACCTGTTTACCTCCCTCCAAATAGTTCTTGGGATCCGCGGGGTTCAAGTCACAATCGTCCTTTGAACCCAATCTCACCAGGTGTAGTCCCCAACAATTTTCATGGTAATGCTATTGTACCTCCCTTCATTCCTGCTTCTGTGACTCCACTTGCACAAATACAGGGAGCTCCAGTGCAGCAATTTGACCGTATGTTTTCAGTACCCACAGCACCACCACCATTATCATCTCTGCCACCTCCTCTACCTGAAATGCCACCTCCGTTGTCGCCACCCCCACCGCCTTTGCCTCAATCACAGCCACCTTTTGTTCCCCCTCCACCTTGTTCCCCCTCCCCTCCTGTGGCTGTTTCAGAACCATCTGTTGTGGAAACTTCTGGACAACCCTCACAATATCAGTGGCAGGGAACATTGTGTAAGAGTGGTGTTCATTACAGTACAGTTAATGCATATAGAGTGGATTCTGATATTTGCAAATATTCCAATGCCACTTCTGAACCTATAGAATGGCCTCTTAAGTTAGACATGACCAAGCGTACAGATTTTCGGCATGTGAAGTCAACATTTACTAGCACCCCACCACATAAGAGGGAAGTGTGTCGATTGATCCCATCTTCTGCAGCCGATCAGAAAGGGTTTCAGGATTTCTTGTCATACTTGAAGCAGAGGGACTGCTCTGGAGTGATCAAGATCCCAGCGGTGAATTCCATGTGGGCCAGACTTCTCTTCATACTTCCATACTCGGAAGAGACTTGCTCTATGCTCTCTATTGCGCCTTGTCAACCTGATTGCCTCATTGCTTTAATATTACCTAAAGAAACGAATTTTGAGTGGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 SPOC

Protein Analysis

1172

Amino Acids

128.74

Weight (kDa)

7.05

Isoelectric Point (pI)

54.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPOC PF07744 1019 - 1164 1.2e-21 SPOC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1431
AccB1I GGYRCC 5 cut(s) 1808, 2197, 2207, 2243, 2465
AccB7I CCANNNNNTGG 1 cut(s) 2350
AccI GTMKAC 1 cut(s) 172
AccII CGCG 6 cut(s) 125, 180, 380, 543, 1903, 2719
AclWI GGATC 6 cut(s) 1049, 1208, 2708, 2721, 3280, 3364
AcoI YGGCCR 1 cut(s) 116
AcsI RAATTY 8 cut(s) 156, 1570, 2120, 2392, 2506, 2658, 3382, 3502
AcuI CTGAAG 2 cut(s) 1794, 2154
AdeI CACNNNGTG 1 cut(s) 2762
AfiI CCNNNNNNNGG 7 cut(s) 289, 519, 1455, 1785, 1841, 2350, 3022
AflII CTTAAG 1 cut(s) 3194
AflIII ACRYGT 1 cut(s) 2298
AhlI ACTAGT 1 cut(s) 899
AjnI CCWGG 6 cut(s) 147, 1454, 2289, 2572, 2596, 2758
Alw21I GWGCWC 2 cut(s) 736, 2851
Alw26I GTCTC 2 cut(s) 626, 3422
AlwI GGATC 6 cut(s) 1049, 1208, 2708, 2721, 3280, 3364
AoxI GGCC 7 cut(s) 3, 116, 291, 1394, 2025, 3188, 3393
ApoI RAATTY 8 cut(s) 156, 1570, 2120, 2392, 2506, 2658, 3382, 3502
Asp700I GAANNNNTTC 1 cut(s) 2011
AspA2I CCTAGG 1 cut(s) 2552
AspLEI GCGC 3 cut(s) 125, 1905, 3454
AspS9I GGNCC 4 cut(s) 1395, 2025, 2213, 3393
AsuHPI GGTGA 7 cut(s) 244, 721, 1476, 1491, 1578, 2748, 3391
AsuII TTCGAA 1 cut(s) 353
AsuNHI GCTAGC 1 cut(s) 973
AvaII GGWCC 1 cut(s) 2213
AvrII CCTAGG 1 cut(s) 2552
BaeGI GKGCMC 1 cut(s) 2200
BamHI GGATCC 1 cut(s) 2713
BanI GGYRCC 5 cut(s) 1808, 2197, 2207, 2243, 2465
BanII GRGCYC 1 cut(s) 2851
BarI GAAGNNNNNNTAC 2 cut(s) 2094, 2126
BauI CACGAG 1 cut(s) 1890
BbsI GAAGAC 1 cut(s) 1437
Bbv12I GWGCWC 2 cut(s) 736, 2851
BbvCI CCTCAGC 1 cut(s) 2638
BceAI ACGGC 3 cut(s) 131, 306, 1835
BciT130I CCWGG 6 cut(s) 149, 1456, 2291, 2574, 2598, 2760
BciVI GTATCC 3 cut(s) 433, 1930, 2371
BclI TGATCA 1 cut(s) 3363
BcoDI GTCTC 2 cut(s) 626, 3422
BcuI ACTAGT 1 cut(s) 899
BfmI CTRYAG 3 cut(s) 2445, 3180, 3297
BfrI CTTAAG 1 cut(s) 3194
BfuAI ACCTGC 1 cut(s) 1431
BfuI GTATCC 3 cut(s) 433, 1930, 2371
BglI GCCNNNNNGGC 1 cut(s) 1886
BglII AGATCT 2 cut(s) 1598, 2249
BlnI CCTAGG 1 cut(s) 2552
Bme1390I CCNGG 6 cut(s) 149, 1456, 2291, 2574, 2598, 2760
Bme18I GGWCC 1 cut(s) 2213
BmgT120I GGNCC 4 cut(s) 1395, 2025, 2213, 3393
BmiI GGNNCC 7 cut(s) 1369, 1810, 2199, 2209, 2245, 2467, 2715
BmrFI CCNGG 6 cut(s) 149, 1456, 2291, 2574, 2598, 2760
BmrI ACTGGG 2 cut(s) 718, 1955
BmsI GCATC 9 cut(s) 892, 986, 994, 1065, 1467, 1555, 1867, 2179, 2442
BmtI GCTAGC 1 cut(s) 977
BmuI ACTGGG 2 cut(s) 718, 1955
BpiI GAAGAC 1 cut(s) 1437
BplI GAGNNNNNCTC 4 cut(s) 2330, 2362, 3419, 3451
BpmI CTGGAG 5 cut(s) 117, 280, 430, 2835, 3378
Bpu10I CCTNAGC 1 cut(s) 2638
Bpu14I TTCGAA 1 cut(s) 353
BpuEI CTTGAG 2 cut(s) 284, 689
BsaAI YACGTR 1 cut(s) 731
BsaBI GATNNNNATC 3 cut(s) 1108, 1212, 3368
BsaXI ACNNNNNCTCC 2 cut(s) 2838, 2868
Bsc4I CCNNNNNNNGG 7 cut(s) 289, 519, 1455, 1785, 1841, 2350, 3022
Bse1I ACTGG 7 cut(s) 413, 724, 1044, 1279, 1950, 2443, 2852
Bse3DI GCAATG 3 cut(s) 958, 2104, 3474
Bse8I GATNNNNATC 3 cut(s) 1108, 1212, 3368
BseBI CCWGG 6 cut(s) 149, 1456, 2291, 2574, 2598, 2760
BseGI GGATG 8 cut(s) 62, 527, 613, 880, 1080, 1210, 1645, 2038
BseJI GATNNNNATC 3 cut(s) 1108, 1212, 3368
BseLI CCNNNNNNNGG 7 cut(s) 289, 519, 1455, 1785, 1841, 2350, 3022
BseMI GCAATG 3 cut(s) 958, 2104, 3474
BseMII CTCAG 6 cut(s) 175, 957, 1740, 2176, 2370, 2652
BseNI ACTGG 7 cut(s) 413, 724, 1044, 1279, 1950, 2443, 2852
BseRI GAGGAG 3 cut(s) 447, 2640, 2910
BseSI GKGCMC 1 cut(s) 2200
BseX3I CGGCCG 1 cut(s) 116
BseYI CCCAGC 2 cut(s) 1747, 3373
BsgI GTGCAG 3 cut(s) 23, 2577, 2876
Bsh1236I CGCG 6 cut(s) 125, 180, 380, 543, 1903, 2719
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 7 cut(s) 5, 118, 293, 1396, 2027, 3190, 3395
BshNI GGYRCC 5 cut(s) 1808, 2197, 2207, 2243, 2465
BsiEI CGRYCG 1 cut(s) 119
BsiHKAI GWGCWC 2 cut(s) 736, 2851
BsiSI CCGG 2 cut(s) 555, 2211
BslFI GGGAC 4 cut(s) 1472, 1651, 2753, 3362
BslI CCNNNNNNNGG 7 cut(s) 289, 519, 1455, 1785, 1841, 2350, 3022
BsmAI GTCTC 2 cut(s) 626, 3422
BsmFI GGGAC 4 cut(s) 1472, 1651, 2753, 3362
BsmI GAATGC 5 cut(s) 455, 648, 2587, 2604, 2613
BsnI GGCC 7 cut(s) 5, 118, 293, 1396, 2027, 3190, 3395
Bsp119I TTCGAA 1 cut(s) 353
Bsp1286I GDGCHC 3 cut(s) 736, 2200, 2851
Bsp1407I TGTACA 1 cut(s) 313
Bsp19I CCATGG 3 cut(s) 1398, 1713, 1812
BspANI GGCC 7 cut(s) 5, 118, 293, 1396, 2027, 3190, 3395
BspCNI CTCAG 6 cut(s) 174, 958, 1739, 2175, 2371, 2651
BspFNI CGCG 6 cut(s) 125, 180, 380, 543, 1903, 2719
BspLI GGNNCC 7 cut(s) 1369, 1810, 2199, 2209, 2245, 2467, 2715
BspMAI CTGCAG 1 cut(s) 3301
BspMI ACCTGC 1 cut(s) 1431
BspOI GCTAGC 1 cut(s) 977
BspPI GGATC 6 cut(s) 1049, 1208, 2708, 2721, 3280, 3364
BspT104I TTCGAA 1 cut(s) 353
BspT107I GGYRCC 5 cut(s) 1808, 2197, 2207, 2243, 2465
BspTI CTTAAG 1 cut(s) 3194
BsrDI GCAATG 3 cut(s) 958, 2104, 3474
BsrGI TGTACA 1 cut(s) 313
BsrI ACTGG 7 cut(s) 413, 724, 1044, 1279, 1950, 2443, 2852
BssSI CACGAG 1 cut(s) 1890
BssT1I CCWWGG 6 cut(s) 1398, 1713, 1812, 1916, 2028, 2552
Bst2BI CACGAG 1 cut(s) 1890
Bst2UI CCWGG 6 cut(s) 149, 1456, 2291, 2574, 2598, 2760
Bst4CI ACNGT 8 cut(s) 637, 747, 1196, 1622, 2492, 2870, 3116, 3121
Bst6I CTCTTC 3 cut(s) 2467, 3410, 3420
BstAFI CTTAAG 1 cut(s) 3194
BstAPI GCANNNNNTGC 1 cut(s) 2610
BstAUI TGTACA 1 cut(s) 313
BstBAI YACGTR 1 cut(s) 731
BstBI TTCGAA 1 cut(s) 353
BstC8I GCNNGC 6 cut(s) 270, 787, 881, 975, 1907, 2464
BstDSI CCRYGG 5 cut(s) 378, 1398, 1713, 1812, 2717
BstEII GGTNACC 1 cut(s) 1497
BstENI CCTNNNNNAGG 1 cut(s) 1839
BstF5I GGATG 8 cut(s) 62, 527, 613, 880, 1080, 1210, 1645, 2038
BstFNI CGCG 6 cut(s) 125, 180, 380, 543, 1903, 2719
BstHHI GCGC 3 cut(s) 125, 1905, 3454
BstMAI GTCTC 2 cut(s) 626, 3422
BstMCI CGRYCG 1 cut(s) 119
BstNI CCWGG 6 cut(s) 149, 1456, 2291, 2574, 2598, 2760
BstNSI RCATGY 2 cut(s) 2302, 3233
BstPI GGTNACC 1 cut(s) 1497
BstSCI CCNGG 6 cut(s) 147, 1454, 2289, 2572, 2596, 2758
BstSFI CTRYAG 3 cut(s) 2445, 3180, 3297
BstSLI GKGCMC 1 cut(s) 2200
BstUI CGCG 6 cut(s) 125, 180, 380, 543, 1903, 2719
BstV2I GAAGAC 1 cut(s) 1437
BstX2I RGATCY 5 cut(s) 1054, 1598, 2249, 2713, 3369
BstYI RGATCY 5 cut(s) 1054, 1598, 2249, 2713, 3369
BstZI CGGCCG 1 cut(s) 116
BsuI GTATCC 3 cut(s) 433, 1930, 2371
BsuRI GGCC 7 cut(s) 5, 118, 293, 1396, 2027, 3190, 3395
BtgI CCRYGG 5 cut(s) 378, 1398, 1713, 1812, 2717
BtgZI GCGATG 2 cut(s) 54, 2156
BtsCI GGATG 8 cut(s) 62, 527, 613, 880, 1080, 1210, 1645, 2038
BtsI GCAGTG 3 cut(s) 363, 504, 1285
BtsIMutI CAGTG 8 cut(s) 363, 504, 1201, 1285, 2085, 2436, 2859, 3083
BveI ACCTGC 1 cut(s) 1431
Cac8I GCNNGC 6 cut(s) 270, 787, 881, 975, 1907, 2464
CfoI GCGC 3 cut(s) 125, 1905, 3454
Cfr13I GGNCC 4 cut(s) 1395, 2025, 2213, 3393
Cfr42I CCGCGG 2 cut(s) 381, 2720
CsiI ACCWGGT 1 cut(s) 2758
CspCI CAANNNNNGTGG 8 cut(s) 352, 387, 2925, 2946, 2960, 2981, 3059, 3094
DraI TTTAAA 2 cut(s) 1126, 1150
DraIII CACNNNGTG 1 cut(s) 2762
EaeI YGGCCR 1 cut(s) 116
EagI CGGCCG 1 cut(s) 116
Eam1104I CTCTTC 3 cut(s) 2467, 3410, 3420
EarI CTCTTC 3 cut(s) 2467, 3410, 3420
EciI GGCGGA 1 cut(s) 75
Ecl136II GAGCTC 1 cut(s) 2849
EclXI CGGCCG 1 cut(s) 116
Eco130I CCWWGG 6 cut(s) 1398, 1713, 1812, 1916, 2028, 2552
Eco24I GRGCYC 1 cut(s) 2851
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 1 cut(s) 2213
Eco52I CGGCCG 1 cut(s) 116
Eco53kI GAGCTC 1 cut(s) 2849
Eco57I CTGAAG 2 cut(s) 1794, 2154
Eco91I GGTNACC 1 cut(s) 1497
EcoICRI GAGCTC 1 cut(s) 2849
EcoNI CCTNNNNNAGG 1 cut(s) 1839
EcoO65I GGTNACC 1 cut(s) 1497
EcoRI GAATTC 3 cut(s) 2392, 2506, 3382
EcoRII CCWGG 6 cut(s) 147, 1454, 2289, 2572, 2596, 2758
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 6 cut(s) 1398, 1713, 1812, 1916, 2028, 2552
EcoT22I ATGCAT 3 cut(s) 405, 2615, 3130
EcoT38I GRGCYC 1 cut(s) 2851
ErhI CCWWGG 6 cut(s) 1398, 1713, 1812, 1916, 2028, 2552
FalI AAGNNNNNCTT 6 cut(s) 596, 628, 762, 794, 910, 942
FaqI GGGAC 4 cut(s) 1472, 1651, 2753, 3362
FauI CCCGC 2 cut(s) 872, 2712
FauNDI CATATG 3 cut(s) 1314, 1660, 2203
FbaI TGATCA 1 cut(s) 3363
FblI GTMKAC 1 cut(s) 172
FokI GGATG 8 cut(s) 49, 514, 620, 887, 1087, 1217, 1632, 2045
FriOI GRGCYC 1 cut(s) 2851
GlaI GCGC 3 cut(s) 124, 1904, 3453
GsaI CCCAGC 2 cut(s) 1751, 3377
GsuI CTGGAG 5 cut(s) 117, 280, 430, 2835, 3378
HaeIII GGCC 7 cut(s) 5, 118, 293, 1396, 2027, 3190, 3395
HapII CCGG 2 cut(s) 555, 2211
HhaI GCGC 3 cut(s) 125, 1905, 3454
Hin6I GCGC 3 cut(s) 123, 1903, 3452
HinP1I GCGC 3 cut(s) 123, 1903, 3452
HincII GTYRAC 5 cut(s) 525, 1310, 2275, 3240, 3461
HindII GTYRAC 5 cut(s) 525, 1310, 2275, 3240, 3461
HindIII AAGCTT 1 cut(s) 1507
HpaII CCGG 2 cut(s) 555, 2211
HphI GGTGA 7 cut(s) 244, 721, 1476, 1491, 1578, 2748, 3391
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 9 cut(s) 27, 232, 589, 598, 1172, 1491, 2050, 2096, 2815
HpyCH4III ACNGT 8 cut(s) 637, 747, 1196, 1622, 2492, 2870, 3116, 3121
HpyCH4IV ACGT 6 cut(s) 169, 730, 993, 1173, 1461, 2529
HpySE526I ACGT 6 cut(s) 169, 730, 993, 1173, 1461, 2529
HspAI GCGC 3 cut(s) 123, 1903, 3452
Ksp22I TGATCA 1 cut(s) 3363
KspI CCGCGG 2 cut(s) 381, 2720
LmnI GCTCC 9 cut(s) 136, 557, 1417, 2353, 2581, 2601, 2647, 2846, 2854
LweI GCATC 9 cut(s) 892, 986, 994, 1065, 1467, 1555, 1867, 2179, 2442
MabI ACCWGGT 1 cut(s) 2758
MaeII ACGT 6 cut(s) 169, 730, 993, 1173, 1461, 2529
MflI RGATCY 5 cut(s) 1054, 1598, 2249, 2713, 3369
MhlI GDGCHC 3 cut(s) 736, 2200, 2851
MlyI GAGTC 5 cut(s) 236, 767, 1669, 2065, 2818
MmeI TCCRAC 3 cut(s) 604, 1052, 1984
Mph1103I ATGCAT 3 cut(s) 405, 2615, 3130
MroXI GAANNNNTTC 1 cut(s) 2011
MseI TTAA 9 cut(s) 219, 828, 1125, 1149, 1505, 2220, 3123, 3195, 3482
MslI CAYNNNNRTG 4 cut(s) 1998, 2238, 2544, 2787
MspA1I CMGCKG 3 cut(s) 380, 2719, 3377
MspCI CTTAAG 1 cut(s) 3194
MspI CCGG 2 cut(s) 555, 2211
MspR9I CCNGG 6 cut(s) 149, 1456, 2291, 2574, 2598, 2760
Mva1269I GAATGC 5 cut(s) 455, 648, 2587, 2604, 2613
MvaI CCWGG 6 cut(s) 149, 1456, 2291, 2574, 2598, 2760
MvnI CGCG 6 cut(s) 125, 180, 380, 543, 1903, 2719
NcoI CCATGG 3 cut(s) 1398, 1713, 1812
NdeI CATATG 3 cut(s) 1314, 1660, 2203
NheI GCTAGC 1 cut(s) 973
NlaIV GGNNCC 7 cut(s) 1369, 1810, 2199, 2209, 2245, 2467, 2715
NmeAIII GCCGAG 1 cut(s) 144
NsiI ATGCAT 3 cut(s) 405, 2615, 3130
NspI RCATGY 2 cut(s) 2302, 3233
NspV TTCGAA 1 cut(s) 353
PasI CCCWGGG 1 cut(s) 1455
PciI ACATGT 1 cut(s) 2298
PctI GAATGC 5 cut(s) 455, 648, 2587, 2604, 2613
PdmI GAANNNNTTC 1 cut(s) 2011
PflMI CCANNNNNTGG 1 cut(s) 2350
PleI GAGTC 5 cut(s) 236, 767, 1669, 2065, 2818
PpsI GAGTC 5 cut(s) 236, 767, 1669, 2065, 2818
Ppu21I YACGTR 1 cut(s) 731
PscI ACATGT 1 cut(s) 2298
Psp124BI GAGCTC 1 cut(s) 2851
Psp6I CCWGG 6 cut(s) 147, 1454, 2289, 2572, 2596, 2758
PspEI GGTNACC 1 cut(s) 1497
PspFI CCCAGC 2 cut(s) 1747, 3373
PspGI CCWGG 6 cut(s) 147, 1454, 2289, 2572, 2596, 2758
PspN4I GGNNCC 7 cut(s) 1369, 1810, 2199, 2209, 2245, 2467, 2715
PspPI GGNCC 4 cut(s) 1395, 2025, 2213, 3393
PstI CTGCAG 1 cut(s) 3301
PsuI RGATCY 5 cut(s) 1054, 1598, 2249, 2713, 3369
RseI CAYNNNNRTG 4 cut(s) 1998, 2238, 2544, 2787
SacI GAGCTC 1 cut(s) 2851
SacII CCGCGG 2 cut(s) 381, 2720
SaqAI TTAA 9 cut(s) 219, 828, 1125, 1149, 1505, 2220, 3123, 3195, 3482
Sau96I GGNCC 4 cut(s) 1395, 2025, 2213, 3393
SchI GAGTC 5 cut(s) 236, 767, 1669, 2065, 2818
ScrFI CCNGG 6 cut(s) 149, 1456, 2291, 2574, 2598, 2760
SduI GDGCHC 3 cut(s) 736, 2200, 2851
SexAI ACCWGGT 1 cut(s) 2758
SfaNI GCATC 9 cut(s) 892, 986, 994, 1065, 1467, 1555, 1867, 2179, 2442
SfcI CTRYAG 3 cut(s) 2445, 3180, 3297
Sfr303I CCGCGG 2 cut(s) 381, 2720
SfuI TTCGAA 1 cut(s) 353
SgrBI CCGCGG 2 cut(s) 381, 2720
SinI GGWCC 1 cut(s) 2213
SmiMI CAYNNNNRTG 4 cut(s) 1998, 2238, 2544, 2787
SmlI CTYRAG 3 cut(s) 263, 668, 3194
SmoI CTYRAG 3 cut(s) 263, 668, 3194
SpeI ACTAGT 1 cut(s) 899
SspI AATATT 2 cut(s) 3158, 3486
SstI GAGCTC 1 cut(s) 2851
StyD4I CCNGG 6 cut(s) 147, 1454, 2289, 2572, 2596, 2758
StyI CCWWGG 6 cut(s) 1398, 1713, 1812, 1916, 2028, 2552
TaaI ACNGT 8 cut(s) 637, 747, 1196, 1622, 2492, 2870, 3116, 3121
TaiI ACGT 6 cut(s) 172, 733, 996, 1176, 1464, 2532
TaqI TCGA 7 cut(s) 190, 353, 867, 1186, 1220, 1234, 3280
TaqII GACCGA 1 cut(s) 2071
TatI WGTACW 4 cut(s) 313, 920, 2398, 3116
TauI GCSGC 4 cut(s) 76, 128, 380, 383
Tru1I TTAA 9 cut(s) 219, 828, 1125, 1149, 1505, 2220, 3123, 3195, 3482
Tru9I TTAA 9 cut(s) 219, 828, 1125, 1149, 1505, 2220, 3123, 3195, 3482
TscAI CASTG 8 cut(s) 370, 511, 1201, 1285, 2092, 2443, 2859, 3083
TspGWI ACGGA 2 cut(s) 960, 2931
TspRI CASTG 8 cut(s) 370, 511, 1201, 1285, 2092, 2443, 2859, 3083
Van91I CCANNNNNTGG 1 cut(s) 2350
Vha464I CTTAAG 1 cut(s) 3194
VpaK11BI GGWCC 1 cut(s) 2213
XagI CCTNNNNNAGG 1 cut(s) 1839
XapI RAATTY 8 cut(s) 156, 1570, 2120, 2392, 2506, 2658, 3382, 3502
XceI RCATGY 2 cut(s) 2302, 3233
XcmI CCANNNNNNNNNTGG 1 cut(s) 2706
XmaJI CCTAGG 1 cut(s) 2552
XmiI GTMKAC 1 cut(s) 172
XmnI GAANNNNTTC 1 cut(s) 2011
Zsp2I ATGCAT 3 cut(s) 405, 2615, 3130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.