Rh6CG499500

SPOC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0008266
Physical Location & Seq
Forward (+)
66368293 .. 66373154
4862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG499500.1

Sequence Viewer

Length: 3120 bp
ATGCATAACTGGAATGTGGGGCTGATAAATAGAGGAGAGGATACTAATGGCTTTGCATTCCAGACTGCGAAATCCTGTATTCTTGGTCTATCGGATATTTGTTGCACTGCTTCCTCGGTGGGGTCAACATCCTCTGTCATTCGCGGTATTTGCTCCGGCGTTTTTCAGAATGTGCTTACCTTCTTTATATCGTCCTTTGAGTGGAAGGATGTCTTTCTGATTGTTGGTAAAGAGACTGTGAGAATGCAAGGTTCTGCTGATATCTTTCTTGAGCTAAAGGAGAAGTTTTCTGATGGAAATGAATCTTCGGTGACTAAGTTATCCCAGTTACGTGCTCTTTGTTTACTGTGGATTTTCTTTTGTTATCCTAAAGACTCACTTGCAGCCTGCTTTGAACTCTTGAAATCCACCTCATCAGAGGGAGTTCTTAAAGGGCAGTATTTTCTAAGTCAAATAACAAGCAAACTCGATAAGGATGGCGGGCATCATTTGAGCAAAACTAGTGATGAATCTAAAACAAGTACAACTTTTGTTGAAACAAGCAACGGAGGCAATGATGTTAGCTCTGAGCAGCTAGCATCAGATGGAAACAACGTCTTTGAAGATGCGATTTCAGTTTCAAATATCTGCTTGTTAGGACTGGTTCTTAGTAAAGATCCATTATTGCAAAGTTGGATGCTCTCAAAATATAAGAAGTTATGTAGATTGCCATCTATTTCAGATTTTAAATCTGCTTTGGAGGGTGTCTTTAAATCTTTTATAGATGTAATGGACGTAGAAGGTAATCGAGCAGACAGTGATGAGGATGATTCTGATTCATCGAGATTTATCAATCGACCATATCTGGTGTCTAGGTTCTCTAACCTACATGAAACTGGCAGTGATGTTTATTCTGATAGAGTGTCAGGTCAACATATGAAAGCTCGTAGCTCTGTAATGCCTCTTGAAGCTAATAGTCACTCAACAGGTTCCAATCAGGATAGTGGCGGTGCAAGGCCCATGGACTTTGAGATTAGGGAGCATGGAGATATGTCACATGGCAGGTCTTCCATGCCCAGGGACGTAGTGAACCACCAGATGCCTTCACCTGTCACAAGGTCACCATTAAGCTTTAGGAGTAATTCGTTTGATGGGAGAAACCACTTTCATATTGAGAAGAACAAAGATGCAAATTTGGATTTTGGTTCACCTCCCATGAGATCTTCAAGTGGAGGTGCAAACAGTTCTTTTGAATCTCCCAAATGTCATCCTGTGTCGTCATATGTGTCCCCCAAGACTCAAATTGTTTGGTATTCTGACGGGGATATTGCAGCCATGGACATTTTCTCAGCTTCTAAACAGCTCTGGCTGGGCTTTTTTGGTTCTGATGTATCTGAAGCCCATGTAAGGTTTCAGTTAGAGAGGTTTGGTGCCATGGAACAATTTTTTTTCTTTCCTCATAAAGGATTTGCCGTAGTTGAGTATAGAAACATTCTTGATGCCATAAAGGCTCGTGAATATACGCGCAGGCATTTTCCTTGGTGCATAAAGTTTATGGATACAGGACTGGGAACTAGAGGTGCTATGAATGGTGTTGCAGTTGGTTCTAGTTGTCATGTTTATGTTGGAAATGTTTCAAGTCAATGGGCCAAGGATGAGATTCTTCACGAATCAAGGAAGGTGCTTCGCAAGACTCCATACTCGGTCACTGATTTCAGCAATGAAGGTGCGGTACTGATGGAATTTGATACTCCTGAAGAAGCTGCTGCTGTGATGGCACATCTCAGACAGCATCGCAAGGAAAGGAGTAACCACAGGGCACCATATGGTGCCGGACCACTTAATGTAGTATCTCACATTGATGGTGCCAGATCTTTACCTACCCCTACCCATGTTGACATCAGAAGCAACCAGGGAAACATGTCTAATACTGCTGCTACCCCATTTGCAGTAAAGCATGAGAGCCACTCAATGGAGCTTGTATCCCCTAGAGTAAAATCTGAGAATCAGGGGAATTCAGTACAAAGTGGATATATGTTTCAGTCAAACAGAGCTGTCACTGGCTCTACAGAGATGCTTGAAGCTGGCACCAGAAGAGTTGATGGTTATGACAGTAACTTAGCTGTGAATTCTACACAAGGAGGTGGTCACGTTGCTTCTCATGCCAATGAACCTAGGTGGTTGTATTCAAAACCTGGAATGGAGCTGCATTCTGCACCAGGGAGCATTCCATGCATTCCTGTACCAACACAGATACCCTCAGCTCCACCTCCTCCACAAATTTCCTCATCTCCATTTATGCGACCTGTTTACCTCCCTCCAAATAGTTCTTGGGATCCACGGGGTTCAAGTCACAATCGTCCTTTGAACCCAATCTCACCAGGTGTAGTCCTCAACAATTTTCATGGTAATGCTATTGTACCTCCCTTCATTCCTGCTTCTGTGACTCCACTTGCACAAATACAGGGAGCTCCAGTGCAGCAATTTGACCGTATGTTTTCAGTACCCACAGCACCACCACCATTATCATCTCTGCCACCTCCTCTACCTGAAATGCCACCTCCGTTGTCGCCACCCCCACCGCCTTTGCCTCAATCACAGCCACCTTTTGTTCCCCCTCCACCTTGTTCCCCCTCCCCTCCTGTGGCTGTTTCAGAACCATCTGTTGTGGAAACTTCTGGACAACCCTCACAATATCAGTGGCAGGGAACATTGTGTAAGAGTGGTGTTCATTACAGTACAGTTAATGCATATAGAGTGGATTCTGATATTTGCAAATATTCCAATGCCACTTCTGAACCTATAGAATGGCCTCTTAAGTTAGACATGACCAAGCGTACAGATTTTCGGCATGTGAAGTCAACATTTACTAGCACCCCACCACATAAGAGGGAAGTGTGTCGATTGATCCCATCTTCTGCAGCCGATCAGAAAGGGTTTCAGGATTTCTTGTCATACTTGAAGCAGAGGGACTGCTCTGGAGTGATCAAGATCCCAGCGGTGAATTCCATGTGGGCCAGACTTCTCTTCATACTTCCATACTCGGAAGAGACTTGCTCTATGCTCTCTATTGCCCCTTGTCAACCTGATTGCCTCATTGCTTTAATATTACCTAAAGAAACGAATTTTGAGTGGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 SPOC

Protein Analysis

1039

Amino Acids

114.19

Weight (kDa)

6.48

Isoelectric Point (pI)

50.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPOC PF07744 886 - 1031 9.7e-22 SPOC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1032
AccB1I GGYRCC 5 cut(s) 1409, 1798, 1808, 1844, 2066
AccB7I CCANNNNNTGG 1 cut(s) 1951
AccII CGCG 2 cut(s) 144, 1504
AciI CCGC 6 cut(s) 144, 480, 987, 1709, 2561, 2978
AclWI GGATC 5 cut(s) 650, 2309, 2322, 2881, 2965
AcsI RAATTY 7 cut(s) 1171, 1721, 1993, 2107, 2259, 2983, 3103
AcuI CTGAAG 2 cut(s) 1395, 1755
AdeI CACNNNGTG 1 cut(s) 2363
AfaI GTAC 8 cut(s) 523, 1713, 2001, 2223, 2400, 2484, 2719, 2818
AfiI CCNNNNNNNGG 7 cut(s) 120, 201, 1056, 1386, 1442, 1951, 2623
AflII CTTAAG 1 cut(s) 2795
AflIII ACRYGT 1 cut(s) 1899
AhlI ACTAGT 1 cut(s) 500
AjnI CCWGG 5 cut(s) 1055, 1890, 2173, 2197, 2359
Alw21I GWGCWC 2 cut(s) 337, 2452
Alw26I GTCTC 2 cut(s) 227, 3023
AlwI GGATC 5 cut(s) 650, 2309, 2322, 2881, 2965
AoxI GGCC 4 cut(s) 995, 1626, 2789, 2994
ApeKI GCWGC 9 cut(s) 383, 571, 1310, 1742, 1745, 1913, 2185, 2458, 2900
ApoI RAATTY 7 cut(s) 1171, 1721, 1993, 2107, 2259, 2983, 3103
Asp700I GAANNNNTTC 1 cut(s) 1612
AspA2I CCTAGG 1 cut(s) 2153
AspLEI GCGC 1 cut(s) 1506
AspS9I GGNCC 4 cut(s) 996, 1626, 1814, 2994
AsuHPI GGTGA 6 cut(s) 322, 1077, 1092, 1179, 2349, 2992
AsuNHI GCTAGC 1 cut(s) 574
AvaII GGWCC 1 cut(s) 1814
AvrII CCTAGG 1 cut(s) 2153
BaeGI GKGCMC 1 cut(s) 1801
BamHI GGATCC 1 cut(s) 2314
BanI GGYRCC 5 cut(s) 1409, 1798, 1808, 1844, 2066
BanII GRGCYC 1 cut(s) 2452
BarI GAAGNNNNNNTAC 2 cut(s) 1695, 1727
BauI CACGAG 1 cut(s) 1491
BbsI GAAGAC 1 cut(s) 1038
Bbv12I GWGCWC 2 cut(s) 337, 2452
BbvCI CCTCAGC 1 cut(s) 2239
BbvI GCAGC 9 cut(s) 395, 583, 1322, 1729, 1732, 1900, 2172, 2470, 2912
BceAI ACGGC 1 cut(s) 1436
BciT130I CCWGG 5 cut(s) 1057, 1892, 2175, 2199, 2361
BciVI GTATCC 3 cut(s) 34, 1531, 1972
BclI TGATCA 1 cut(s) 2964
BcoDI GTCTC 2 cut(s) 227, 3023
BcuI ACTAGT 1 cut(s) 500
BfaI CTAG 8 cut(s) 501, 575, 852, 1554, 1587, 1968, 2154, 2850
BfmI CTRYAG 3 cut(s) 2046, 2781, 2898
BfrI CTTAAG 1 cut(s) 2795
BfuAI ACCTGC 1 cut(s) 1032
BfuI GTATCC 3 cut(s) 34, 1531, 1972
BglI GCCNNNNNGGC 1 cut(s) 1487
BglII AGATCT 2 cut(s) 1199, 1850
BisI GCNGC 9 cut(s) 384, 572, 1311, 1743, 1746, 1914, 2186, 2459, 2901
BlnI CCTAGG 1 cut(s) 2153
BlsI GCNGC 9 cut(s) 385, 573, 1312, 1744, 1747, 1915, 2187, 2460, 2902
Bme1390I CCNGG 5 cut(s) 1057, 1892, 2175, 2199, 2361
Bme18I GGWCC 1 cut(s) 1814
BmgT120I GGNCC 4 cut(s) 996, 1626, 1814, 2994
BmiI GGNNCC 7 cut(s) 970, 1411, 1800, 1810, 1846, 2068, 2316
BmrFI CCNGG 5 cut(s) 1057, 1892, 2175, 2199, 2361
BmrI ACTGGG 2 cut(s) 319, 1556
BmsI GCATC 9 cut(s) 493, 587, 595, 666, 1068, 1156, 1468, 1780, 2043
BmtI GCTAGC 1 cut(s) 578
BmuI ACTGGG 2 cut(s) 319, 1556
BpiI GAAGAC 1 cut(s) 1038
BplI GAGNNNNNCTC 4 cut(s) 1931, 1963, 3020, 3052
BpmI CTGGAG 2 cut(s) 2436, 2979
Bpu10I CCTNAGC 1 cut(s) 2239
BpuEI CTTGAG 1 cut(s) 290
BsaAI YACGTR 1 cut(s) 332
BsaBI GATNNNNATC 2 cut(s) 709, 2969
BsaXI ACNNNNNCTCC 2 cut(s) 2439, 2469
Bsc4I CCNNNNNNNGG 7 cut(s) 120, 201, 1056, 1386, 1442, 1951, 2623
Bse1I ACTGG 7 cut(s) 14, 325, 645, 880, 1551, 2044, 2453
Bse3DI GCAATG 3 cut(s) 559, 1705, 3075
Bse8I GATNNNNATC 2 cut(s) 709, 2969
BseBI CCWGG 5 cut(s) 1057, 1892, 2175, 2199, 2361
BseGI GGATG 7 cut(s) 128, 214, 481, 681, 811, 1246, 1639
BseJI GATNNNNATC 2 cut(s) 709, 2969
BseLI CCNNNNNNNGG 7 cut(s) 120, 201, 1056, 1386, 1442, 1951, 2623
BseMI GCAATG 3 cut(s) 559, 1705, 3075
BseMII CTCAG 5 cut(s) 558, 1341, 1777, 1971, 2253
BseNI ACTGG 7 cut(s) 14, 325, 645, 880, 1551, 2044, 2453
BseRI GAGGAG 3 cut(s) 48, 2241, 2511
BseSI GKGCMC 1 cut(s) 1801
BseXI GCAGC 9 cut(s) 395, 583, 1322, 1729, 1732, 1900, 2172, 2470, 2912
BseYI CCCAGC 2 cut(s) 1348, 2974
BsgI GTGCAG 2 cut(s) 2178, 2477
Bsh1236I CGCG 2 cut(s) 144, 1504
BshFI GGCC 4 cut(s) 997, 1628, 2791, 2996
BshNI GGYRCC 5 cut(s) 1409, 1798, 1808, 1844, 2066
BsiHKAI GWGCWC 2 cut(s) 337, 2452
BsiSI CCGG 2 cut(s) 156, 1812
BslFI GGGAC 3 cut(s) 1073, 1252, 2963
BslI CCNNNNNNNGG 7 cut(s) 120, 201, 1056, 1386, 1442, 1951, 2623
BsmAI GTCTC 2 cut(s) 227, 3023
BsmFI GGGAC 3 cut(s) 1073, 1252, 2963
BsmI GAATGC 5 cut(s) 56, 249, 2188, 2205, 2214
BsnI GGCC 4 cut(s) 997, 1628, 2791, 2996
Bsp1286I GDGCHC 3 cut(s) 337, 1801, 2452
Bsp143I GATC 8 cut(s) 655, 1199, 1850, 2314, 2886, 2905, 2964, 2970
Bsp19I CCATGG 3 cut(s) 999, 1314, 1413
BspACI CCGC 6 cut(s) 144, 480, 987, 1709, 2561, 2978
BspANI GGCC 4 cut(s) 997, 1628, 2791, 2996
BspCNI CTCAG 5 cut(s) 559, 1340, 1776, 1972, 2252
BspFNI CGCG 2 cut(s) 144, 1504
BspLI GGNNCC 7 cut(s) 970, 1411, 1800, 1810, 1846, 2068, 2316
BspMAI CTGCAG 1 cut(s) 2902
BspMI ACCTGC 1 cut(s) 1032
BspOI GCTAGC 1 cut(s) 578
BspPI GGATC 5 cut(s) 650, 2309, 2322, 2881, 2965
BspT107I GGYRCC 5 cut(s) 1409, 1798, 1808, 1844, 2066
BspTI CTTAAG 1 cut(s) 2795
BsrDI GCAATG 3 cut(s) 559, 1705, 3075
BsrI ACTGG 7 cut(s) 14, 325, 645, 880, 1551, 2044, 2453
BssMI GATC 8 cut(s) 655, 1199, 1850, 2314, 2886, 2905, 2964, 2970
BssSI CACGAG 1 cut(s) 1491
BssT1I CCWWGG 6 cut(s) 999, 1314, 1413, 1517, 1629, 2153
Bst2BI CACGAG 1 cut(s) 1491
Bst2UI CCWGG 5 cut(s) 1057, 1892, 2175, 2199, 2361
Bst4CI ACNGT 8 cut(s) 238, 348, 797, 1223, 2093, 2471, 2717, 2722
Bst6I CTCTTC 3 cut(s) 2068, 3011, 3021
BstAFI CTTAAG 1 cut(s) 2795
BstAPI GCANNNNNTGC 1 cut(s) 2211
BstBAI YACGTR 1 cut(s) 332
BstC8I GCNNGC 5 cut(s) 388, 482, 576, 1508, 2065
BstDEI CTNAG 9 cut(s) 315, 446, 567, 647, 1327, 1763, 1980, 2098, 2239
BstDSI CCRYGG 4 cut(s) 999, 1314, 1413, 2318
BstEII GGTNACC 1 cut(s) 1098
BstENI CCTNNNNNAGG 1 cut(s) 1440
BstF5I GGATG 7 cut(s) 128, 214, 481, 681, 811, 1246, 1639
BstFNI CGCG 2 cut(s) 144, 1504
BstHHI GCGC 1 cut(s) 1506
BstKTI GATC 8 cut(s) 658, 1202, 1853, 2317, 2889, 2908, 2967, 2973
BstMAI GTCTC 2 cut(s) 227, 3023
BstMBI GATC 8 cut(s) 655, 1199, 1850, 2314, 2886, 2905, 2964, 2970
BstMWI GCNNNNNNNGC 6 cut(s) 150, 549, 1487, 1754, 2141, 2211
BstNI CCWGG 5 cut(s) 1057, 1892, 2175, 2199, 2361
BstNSI RCATGY 2 cut(s) 1903, 2834
BstPI GGTNACC 1 cut(s) 1098
BstSCI CCNGG 5 cut(s) 1055, 1890, 2173, 2197, 2359
BstSFI CTRYAG 3 cut(s) 2046, 2781, 2898
BstSLI GKGCMC 1 cut(s) 1801
BstUI CGCG 2 cut(s) 144, 1504
BstV1I GCAGC 9 cut(s) 395, 583, 1322, 1729, 1732, 1900, 2172, 2470, 2912
BstV2I GAAGAC 1 cut(s) 1038
BstX2I RGATCY 5 cut(s) 655, 1199, 1850, 2314, 2970
BstYI RGATCY 5 cut(s) 655, 1199, 1850, 2314, 2970
BsuI GTATCC 3 cut(s) 34, 1531, 1972
BsuRI GGCC 4 cut(s) 997, 1628, 2791, 2996
BtgI CCRYGG 4 cut(s) 999, 1314, 1413, 2318
BtgZI GCGATG 1 cut(s) 1757
BtsCI GGATG 7 cut(s) 128, 214, 481, 681, 811, 1246, 1639
BtsI GCAGTG 2 cut(s) 105, 886
BtsIMutI CAGTG 7 cut(s) 105, 802, 886, 1686, 2037, 2460, 2684
BveI ACCTGC 1 cut(s) 1032
Cac8I GCNNGC 5 cut(s) 388, 482, 576, 1508, 2065
CfoI GCGC 1 cut(s) 1506
Cfr13I GGNCC 4 cut(s) 996, 1626, 1814, 2994
CsiI ACCWGGT 1 cut(s) 2359
Csp6I GTAC 8 cut(s) 522, 1712, 2000, 2222, 2399, 2483, 2718, 2817
CspCI CAANNNNNGTGG 6 cut(s) 2526, 2547, 2561, 2582, 2660, 2695
CviQI GTAC 8 cut(s) 522, 1712, 2000, 2222, 2399, 2483, 2718, 2817
DdeI CTNAG 9 cut(s) 315, 446, 567, 647, 1327, 1763, 1980, 2098, 2239
DpnI GATC 8 cut(s) 657, 1201, 1852, 2316, 2888, 2907, 2966, 2972
DpnII GATC 8 cut(s) 655, 1199, 1850, 2314, 2886, 2905, 2964, 2970
DraI TTTAAA 2 cut(s) 727, 751
DraIII CACNNNGTG 1 cut(s) 2363
Eam1104I CTCTTC 3 cut(s) 2068, 3011, 3021
EarI CTCTTC 3 cut(s) 2068, 3011, 3021
Ecl136II GAGCTC 1 cut(s) 2450
Eco130I CCWWGG 6 cut(s) 999, 1314, 1413, 1517, 1629, 2153
Eco24I GRGCYC 1 cut(s) 2452
Eco32I GATATC 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 1814
Eco53kI GAGCTC 1 cut(s) 2450
Eco57I CTGAAG 2 cut(s) 1395, 1755
Eco91I GGTNACC 1 cut(s) 1098
EcoICRI GAGCTC 1 cut(s) 2450
EcoNI CCTNNNNNAGG 1 cut(s) 1440
EcoO65I GGTNACC 1 cut(s) 1098
EcoRI GAATTC 3 cut(s) 1993, 2107, 2983
EcoRII CCWGG 5 cut(s) 1055, 1890, 2173, 2197, 2359
EcoRV GATATC 1 cut(s) 262
EcoT14I CCWWGG 6 cut(s) 999, 1314, 1413, 1517, 1629, 2153
EcoT22I ATGCAT 3 cut(s) 6, 2216, 2731
EcoT38I GRGCYC 1 cut(s) 2452
ErhI CCWWGG 6 cut(s) 999, 1314, 1413, 1517, 1629, 2153
FalI AAGNNNNNCTT 6 cut(s) 197, 229, 363, 395, 511, 543
FaqI GGGAC 3 cut(s) 1073, 1252, 2963
FauI CCCGC 1 cut(s) 473
FauNDI CATATG 3 cut(s) 915, 1261, 1804
FbaI TGATCA 1 cut(s) 2964
Fnu4HI GCNGC 9 cut(s) 384, 572, 1311, 1743, 1746, 1914, 2186, 2459, 2901
FokI GGATG 7 cut(s) 115, 221, 488, 688, 818, 1233, 1646
FriOI GRGCYC 1 cut(s) 2452
Fsp4HI GCNGC 9 cut(s) 384, 572, 1311, 1743, 1746, 1914, 2186, 2459, 2901
FspBI CTAG 8 cut(s) 501, 575, 852, 1554, 1587, 1968, 2154, 2850
GlaI GCGC 1 cut(s) 1505
GluI GCNGC 9 cut(s) 384, 572, 1311, 1743, 1746, 1914, 2186, 2459, 2901
GsaI CCCAGC 2 cut(s) 1352, 2978
GsuI CTGGAG 2 cut(s) 2436, 2979
HaeIII GGCC 4 cut(s) 997, 1628, 2791, 2996
HapII CCGG 2 cut(s) 156, 1812
HhaI GCGC 1 cut(s) 1506
Hin6I GCGC 1 cut(s) 1504
HinP1I GCGC 1 cut(s) 1504
HincII GTYRAC 5 cut(s) 126, 911, 1876, 2841, 3062
HindII GTYRAC 5 cut(s) 126, 911, 1876, 2841, 3062
HindIII AAGCTT 1 cut(s) 1108
HpaII CCGG 2 cut(s) 156, 1812
HphI GGTGA 6 cut(s) 322, 1077, 1092, 1179, 2349, 2992
Hpy166II GTNNAC 9 cut(s) 126, 344, 911, 1069, 1187, 1876, 2290, 2841, 3062
Hpy8I GTNNAC 9 cut(s) 126, 344, 911, 1069, 1187, 1876, 2290, 2841, 3062
HpyAV CCTTC 7 cut(s) 190, 199, 773, 1092, 1651, 1697, 2416
HpyCH4III ACNGT 8 cut(s) 238, 348, 797, 1223, 2093, 2471, 2717, 2722
HpyCH4IV ACGT 5 cut(s) 331, 594, 774, 1062, 2130
HpyF10VI GCNNNNNNNGC 6 cut(s) 150, 549, 1487, 1754, 2141, 2211
HpyF3I CTNAG 9 cut(s) 315, 446, 567, 647, 1327, 1763, 1980, 2098, 2239
HpySE526I ACGT 5 cut(s) 331, 594, 774, 1062, 2130
HspAI GCGC 1 cut(s) 1504
Ksp22I TGATCA 1 cut(s) 2964
Kzo9I GATC 8 cut(s) 655, 1199, 1850, 2314, 2886, 2905, 2964, 2970
LmnI GCTCC 8 cut(s) 158, 1018, 1954, 2182, 2202, 2248, 2447, 2455
Lsp1109I GCAGC 9 cut(s) 395, 583, 1322, 1729, 1732, 1900, 2172, 2470, 2912
LweI GCATC 9 cut(s) 493, 587, 595, 666, 1068, 1156, 1468, 1780, 2043
MabI ACCWGGT 1 cut(s) 2359
MaeI CTAG 8 cut(s) 501, 575, 852, 1554, 1587, 1968, 2154, 2850
MaeII ACGT 5 cut(s) 331, 594, 774, 1062, 2130
MalI GATC 8 cut(s) 657, 1201, 1852, 2316, 2888, 2907, 2966, 2972
MboI GATC 8 cut(s) 655, 1199, 1850, 2314, 2886, 2905, 2964, 2970
MflI RGATCY 5 cut(s) 655, 1199, 1850, 2314, 2970
MhlI GDGCHC 3 cut(s) 337, 1801, 2452
MlyI GAGTC 4 cut(s) 368, 1270, 1666, 2419
MmeI TCCRAC 2 cut(s) 653, 1585
Mph1103I ATGCAT 3 cut(s) 6, 2216, 2731
MroXI GAANNNNTTC 1 cut(s) 1612
MseI TTAA 8 cut(s) 429, 726, 750, 1106, 1821, 2724, 2796, 3083
MslI CAYNNNNRTG 4 cut(s) 1599, 1839, 2145, 2388
MspA1I CMGCKG 1 cut(s) 2978
MspCI CTTAAG 1 cut(s) 2795
MspI CCGG 2 cut(s) 156, 1812
MspR9I CCNGG 5 cut(s) 1057, 1892, 2175, 2199, 2361
Mva1269I GAATGC 5 cut(s) 56, 249, 2188, 2205, 2214
MvaI CCWGG 5 cut(s) 1057, 1892, 2175, 2199, 2361
MvnI CGCG 2 cut(s) 144, 1504
MwoI GCNNNNNNNGC 6 cut(s) 150, 549, 1487, 1754, 2141, 2211
NcoI CCATGG 3 cut(s) 999, 1314, 1413
NdeI CATATG 3 cut(s) 915, 1261, 1804
NdeII GATC 8 cut(s) 655, 1199, 1850, 2314, 2886, 2905, 2964, 2970
NheI GCTAGC 1 cut(s) 574
NlaIV GGNNCC 7 cut(s) 970, 1411, 1800, 1810, 1846, 2068, 2316
NsiI ATGCAT 3 cut(s) 6, 2216, 2731
NspI RCATGY 2 cut(s) 1903, 2834
PasI CCCWGGG 1 cut(s) 1056
PciI ACATGT 1 cut(s) 1899
PctI GAATGC 5 cut(s) 56, 249, 2188, 2205, 2214
PdmI GAANNNNTTC 1 cut(s) 1612
PfeI GAWTC 9 cut(s) 302, 509, 809, 815, 1232, 1639, 1649, 1984, 2741
PflMI CCANNNNNTGG 1 cut(s) 1951
PkrI GCNGC 9 cut(s) 385, 573, 1312, 1744, 1747, 1915, 2187, 2460, 2902
PleI GAGTC 4 cut(s) 368, 1270, 1666, 2419
PpsI GAGTC 4 cut(s) 368, 1270, 1666, 2419
Ppu21I YACGTR 1 cut(s) 332
PscI ACATGT 1 cut(s) 1899
Psp124BI GAGCTC 1 cut(s) 2452
Psp6I CCWGG 5 cut(s) 1055, 1890, 2173, 2197, 2359
PspEI GGTNACC 1 cut(s) 1098
PspFI CCCAGC 2 cut(s) 1348, 2974
PspGI CCWGG 5 cut(s) 1055, 1890, 2173, 2197, 2359
PspN4I GGNNCC 7 cut(s) 970, 1411, 1800, 1810, 1846, 2068, 2316
PspPI GGNCC 4 cut(s) 996, 1626, 1814, 2994
PstI CTGCAG 1 cut(s) 2902
PsuI RGATCY 5 cut(s) 655, 1199, 1850, 2314, 2970
RsaI GTAC 8 cut(s) 523, 1713, 2001, 2223, 2400, 2484, 2719, 2818
RsaNI GTAC 8 cut(s) 522, 1712, 2000, 2222, 2399, 2483, 2718, 2817
RseI CAYNNNNRTG 4 cut(s) 1599, 1839, 2145, 2388
SacI GAGCTC 1 cut(s) 2452
SaqAI TTAA 8 cut(s) 429, 726, 750, 1106, 1821, 2724, 2796, 3083
SatI GCNGC 9 cut(s) 384, 572, 1311, 1743, 1746, 1914, 2186, 2459, 2901
Sau3AI GATC 8 cut(s) 655, 1199, 1850, 2314, 2886, 2905, 2964, 2970
Sau96I GGNCC 4 cut(s) 996, 1626, 1814, 2994
SchI GAGTC 4 cut(s) 368, 1270, 1666, 2419
ScrFI CCNGG 5 cut(s) 1057, 1892, 2175, 2199, 2361
SduI GDGCHC 3 cut(s) 337, 1801, 2452
SexAI ACCWGGT 1 cut(s) 2359
SfaNI GCATC 9 cut(s) 493, 587, 595, 666, 1068, 1156, 1468, 1780, 2043
SfcI CTRYAG 3 cut(s) 2046, 2781, 2898
SinI GGWCC 1 cut(s) 1814
SmiMI CAYNNNNRTG 4 cut(s) 1599, 1839, 2145, 2388
SmlI CTYRAG 2 cut(s) 269, 2795
SmoI CTYRAG 2 cut(s) 269, 2795
SpeI ACTAGT 1 cut(s) 500
SsiI CCGC 6 cut(s) 144, 480, 987, 1709, 2561, 2978
SspI AATATT 2 cut(s) 2759, 3087
SspMI CTAG 8 cut(s) 501, 575, 852, 1554, 1587, 1968, 2154, 2850
SstI GAGCTC 1 cut(s) 2452
StyD4I CCNGG 5 cut(s) 1055, 1890, 2173, 2197, 2359
StyI CCWWGG 6 cut(s) 999, 1314, 1413, 1517, 1629, 2153
TaaI ACNGT 8 cut(s) 238, 348, 797, 1223, 2093, 2471, 2717, 2722
TaiI ACGT 5 cut(s) 334, 597, 777, 1065, 2133
TaqI TCGA 5 cut(s) 468, 787, 821, 835, 2881
TaqII GACCGA 1 cut(s) 1672
TatI WGTACW 3 cut(s) 521, 1999, 2717
TfiI GAWTC 9 cut(s) 302, 509, 809, 815, 1232, 1639, 1649, 1984, 2741
Tru1I TTAA 8 cut(s) 429, 726, 750, 1106, 1821, 2724, 2796, 3083
Tru9I TTAA 8 cut(s) 429, 726, 750, 1106, 1821, 2724, 2796, 3083
TscAI CASTG 7 cut(s) 112, 802, 886, 1693, 2044, 2460, 2684
TseI GCWGC 9 cut(s) 383, 571, 1310, 1742, 1745, 1913, 2185, 2458, 2900
TspGWI ACGGA 2 cut(s) 561, 2532
TspRI CASTG 7 cut(s) 112, 802, 886, 1693, 2044, 2460, 2684
Van91I CCANNNNNTGG 1 cut(s) 1951
Vha464I CTTAAG 1 cut(s) 2795
VpaK11BI GGWCC 1 cut(s) 1814
XagI CCTNNNNNAGG 1 cut(s) 1440
XapI RAATTY 7 cut(s) 1171, 1721, 1993, 2107, 2259, 2983, 3103
XceI RCATGY 2 cut(s) 1903, 2834
XcmI CCANNNNNNNNNTGG 1 cut(s) 2307
XmaJI CCTAGG 1 cut(s) 2153
XmnI GAANNNNTTC 1 cut(s) 1612
XspI CTAG 8 cut(s) 501, 575, 852, 1554, 1587, 1968, 2154, 2850
Zsp2I ATGCAT 3 cut(s) 6, 2216, 2731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.