MD08G1086000.v1.1

SPOC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008266
Physical Location & Seq
Reverse (-)
7138002 .. 7143991
5990 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1086000.v1.1.491

Sequence Viewer

Length: 3456 bp
ATGGACTCGGGAGAGCAACCTCCGAAGAAGCGGAAGCTGCACGAATCCCAACCAGAACTGCCTCCATCGCCGCCATTATTACAACCACCGCCACCACTGCAAACCCTAGCCCCGCCGCGGAGCGTGGGCGCTCCGCAATCTCTGTCTCAGGAGGAGATTATGAAGAAACGGAGATACAGAGACGAGATTCGAAGTGTATACGATTGCTACAAGCGGATCAAGTTTTGCCTTTCGAAGAATGACTCTGCTCTCACGCCGGAGCTCGAGCAAGCTTATCTCTCTCTCATCACTGCTTCCAGAGGTTGTACGAGTGTACAACGCATTGTTGCTGATCTCATTCCTCGGTATGCTTCAAAATGTCCAACTGCCCTTGAAGCTGCAGGGAAAGTTGTTATAAATATGTATAACTGGAGCATGGCAGTAATAAAGAGAGGAGAGGATGCTGATGGTGTTGCATTTCAGACTGCTAAATCCTGTGTTCTTGGTCTAGCTGATATTTGCTGCACTGCATCTTCAGTGGCACCAACATCATCCGTCATTCGGGGGATTTGCTCTGCAGTTTTCCAGAATGTGCTCACCTTCTTCATATCCTCCTGTGAGGGAAAGGATGTCTTTCTTATTGTTGGGAAAGAAACTGTGAGAATACAAGATTCCTCTCAAAAATTTTCTGAGCTAAAGCAAAAGGTTTTAGATGAAAATGAATCGTCACCTATTAAATTATCCAAGTTATGTGCTCTCAGTTTAATATGGATTCTCTTTAGTTATCCACAAAAATTACTCGCAGCCTGGTTTGAACTCTTTAAATCCAGTGCATCGGATGGAGTTCTGAAAGGGCAGTATTTTCTAAGGCAAATGACAAGCAGGCTTGATAATGATGGTGGATATCCTTTCGGTAAAACTGGTGATGAATCTAAATCAAGTACAGGTTATACTGAATCTAGCACCAGAGGCTGCGAGGTTAGCTCTGAGCAGGTAGCATCAGATGGCAATCATGTCTATGGGGATGCATCAACAATTTCAGAGAGCTGCTTGTTAGGACTGGTTCTTAGCAAAGATCCGTCATTGCAAAGTTGGATGTTCTCAAAGTATAAAAAGTTATGTAAATCGTCGTCTTCCAAAGTTTTTAATGATATTAAATCTTCTTTGGAAGATGTATTTAATTCATTTTTAGGGCGAATGGATGTGGGAGATGATCAAGTGGATAGTGATGAGGATGATTCTGATCCATCCAGATTTATTGAACGAGCATATCTGGTTCCTAGGTTCTCTAACCAACATGAGGCCTGTAGTGAACTGTATGGAAAAGATAGTTCCAATCAGGAGAGTTGGGGAACAAGGTCCACAAACTCTGAGATTAGGGAGCATGGAGATATGTCACATGGCAGGTCTTCTGTGCCAAGGGACCTAATGAACCACCAGGTTCTTTCACCTGTCACAAGATCTCCACTAAATTTCAGGAGTAATTCATTTGATGGGAGAAAACATGGTCATCTTGACAAGAATCAAGATGCAATGGATTTTGGCTCACCTCTCATGAGATCTTCAAGTGGTGGTGTAAATAGTTCTTTTGAATCTCCTAGACCTCATATGGTGTCACCATATACTTCCACAACAACTCAAATTGTTTGGTATTCGGATGGGGATATTGGAGCCATGGATATTTTCTCCGCCTCTAAGCAGCTCTGGCTGGGCTTTTCAGGATCTGATGCGTCTGAAGCTCATGTAAGGTTTCAGTTAGAGAGGTTTGGTGCAGTAGAACAATTTATTTTCTTCCCACTCAAGGGGTTTGCCTTGGTAGAGTACAGAAACATTGTGGATGCCATAAAGGCTCGGGAATATATGCGTGGACATTTTCCTTGGCACGTGAAGTTTATGGATATAGGACTGGGAACTAGAGGTACCATGAATGGCATTGCAGTTGGTTCTAGTTGCCATGTTTATGTTGGAAATGTATTAAGTCAATGGGCCAAGGATGAGACTCTGCATGAATCAAGAAAAGTGATTTACAAGGGTCCTTACATGGTCACTGATCTTAGCAATGAAGGTGCGTTACTGATGGAATTTGATACTCCTGAAGAAGCTGCTGCGGTTATGGCACATCTCAGACAACATCGCAAGGAAAGGAGTAACCACAGGCCACCATATGGTGCAGGGCCAGCTAATGTCGTAATATCCCAAACTGATGGTGCAAGATCTGCACCTACCCCTACTCATATTGACATAAGAAGCAGCACCCCTGGAAACATGTCCAGTAGTCATGTTGCTGCACCGTTTTCAGTAAATCATGAGAGCCACCCCATGGAGCTTGCATCCCCTAGAGTAACATCCGAGAATCAGGGGAATACTGTACAGAGTGGATATTTATTTCAGTCAAAGAGAGCTACTGGCTCCACGGAGATGCTTGAATCTGGCACCCCAAAGCTTGATGGTTATGACAATAACTCACCTGTGGATCCTTCACAAGGTAGTCATGCCATTTCTCGTGCTGCTGAACAAAAGTGGATGTATGCAAAACCTGGAATGGAGCTGCATTCCGCACCAGGGAGCATTCCTTGTGTTCCTGTGCCAACACAGGGACCCTGTGTCCCACCTCCACTCCAAATTCACTCATCTCCATTTATACGGCCTGTTTACCCCCCTCCAAGTTGTTCTTGGGATCCACGGGGTGTAAATCATAATCCTCCTTTGAACCCAATCTCACCCGGTGTAATGCCCAACAATTTTCATGGGAATGCTATTGTAGCTCCCTTCATTCCTGCTTCTGTGACTCCACTTGCACAAATACAAGGAACTCCAGCACAGCAATTTGACCAAATGTTTTCAGTACCGATAATTCCACCACCGTTATCATCTCAACCACCTCCTCTGCCTGAAATGCCACCTCCAGTGCCCCCATCTCCACCACCTCTGCCTCAGTCGCAGCCGCCTTTTGTTCCTCCTCCTCCCCATTCACCTCCTCCACCTGTTCCAGAATCATCTGGTGTGGAAAGTTCTGGACAACGCTCTCAGTATCAGTGGCAGGGAATGTTATGCAAAAGTGGTGTTCATTACTGTACTGTTTATGCGCATAGGGTGGATTCAGATATATGCAAATATTTGAATGCCATTTCTGAACCTATAGAATGGCCTGCTAAGTTAGACATGACCAAGCGTACAGATTTTCGGCATGTGAAGTCAACATTTACTAGCACCCCACCACATAAAAGGGAGGTATGCAGATTGATCCCAGCTTCTGCAGGCGACCACAAGGGCTTTCAGGATTTCGTTTCATACTTGAAGCAGAGGGATTGCTCAGGAGTGATCAAGATCCCGGCTGTGAAATCCTTATGGGCGAGGCTTCTGTTCATACTTCCACACTCTGATGACATTTGTGCTATGCTCTCTATTGCACCGAGTCCTCCTGATTGTCTCATTGCTCTAATATTACCTAAAGAAACAAACTTCGAGTGGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 SPOC

Protein Analysis

1152

Amino Acids

126.58

Weight (kDa)

6.64

Isoelectric Point (pI)

57.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPOC PF07744 998 - 1143 3.1e-22 SPOC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 395
Acc16I TGCGCA 1 cut(s) 3066
Acc36I ACCTGC 2 cut(s) 961, 1374
Acc65I GGTACC 1 cut(s) 1898
AccB1I GGYRCC 3 cut(s) 520, 1898, 2411
AccB7I CCANNNNNTGG 2 cut(s) 2144, 2299
AccI GTMKAC 1 cut(s) 198
AccII CGCG 1 cut(s) 118
AcsI RAATTY 4 cut(s) 662, 1450, 2060, 2601
AcuI CTGAAG 3 cut(s) 498, 1734, 2094
AcvI CACGTG 1 cut(s) 1864
AdeI CACNNNGTG 2 cut(s) 2666, 2705
AfaI GTAC 9 cut(s) 307, 315, 922, 1802, 1900, 2349, 2826, 3055, 3154
AflIII ACRYGT 1 cut(s) 2244
AhdI GACNNNNNGTC 1 cut(s) 2582
AjnI CCWGG 5 cut(s) 785, 1416, 2236, 2515, 2539
Alw21I GWGCWC 3 cut(s) 264, 576, 736
Alw26I GTCTC 4 cut(s) 150, 174, 1970, 3413
AlwNI CAGNNNCTG 3 cut(s) 951, 1703, 3233
Ama87I CYCGRG 3 cut(s) 7, 263, 1830
AoxI GGCC 6 cut(s) 1281, 1965, 2135, 2153, 2624, 3125
ApoI RAATTY 4 cut(s) 662, 1450, 2060, 2601
Asp718I GGTACC 1 cut(s) 1898
AspA2I CCTAGG 1 cut(s) 1259
AspLEI GCGC 2 cut(s) 131, 3067
AspS9I GGNCC 6 cut(s) 1338, 1402, 1965, 2012, 2153, 2576
AsuC2I CCSGG 2 cut(s) 2703, 3311
AsuHPI GGTGA 9 cut(s) 568, 699, 914, 1419, 1518, 1587, 2436, 2691, 2943
AsuII TTCGAA 2 cut(s) 190, 233
AvaI CYCGRG 3 cut(s) 7, 263, 1830
AvaII GGWCC 4 cut(s) 1338, 1402, 2012, 2576
AvrII CCTAGG 1 cut(s) 1259
BaeGI GKGCMC 1 cut(s) 2892
BamHI GGATCC 2 cut(s) 2452, 2656
BanI GGYRCC 3 cut(s) 520, 1898, 2411
BanII GRGCYC 1 cut(s) 264
BarI GAAGNNNNNNTAC 2 cut(s) 2034, 2066
BauI CACGAG 1 cut(s) 2481
BbrPI CACGTG 1 cut(s) 1864
BbsI GAAGAC 2 cut(s) 1104, 1380
Bbv12I GWGCWC 3 cut(s) 264, 576, 736
BceAI ACGGC 1 cut(s) 2639
BciT130I CCWGG 5 cut(s) 787, 1418, 2238, 2517, 2541
BclI TGATCA 2 cut(s) 1192, 3300
BcnI CCSGG 2 cut(s) 2703, 3311
BcoDI GTCTC 4 cut(s) 150, 174, 1970, 3413
BfaI CTAG 9 cut(s) 107, 488, 939, 1260, 1578, 1893, 1926, 2316, 3186
BfmI CTRYAG 5 cut(s) 378, 555, 1285, 3117, 3234
BfoI RGCGCY 1 cut(s) 132
BfuAI ACCTGC 2 cut(s) 961, 1374
BglI GCCNNNNNGGC 1 cut(s) 1826
BglII AGATCT 3 cut(s) 1439, 1538, 2192
BlnI CCTAGG 1 cut(s) 1259
Bme1390I CCNGG 7 cut(s) 787, 1418, 2238, 2517, 2541, 2703, 3311
Bme18I GGWCC 4 cut(s) 1338, 1402, 2012, 2576
BmeRI GACNNNNNGTC 1 cut(s) 2582
BmeT110I CYCGRG 3 cut(s) 7, 263, 1830
BmgT120I GGNCC 6 cut(s) 1338, 1402, 1965, 2012, 2153, 2576
BmrFI CCNGG 7 cut(s) 787, 1418, 2238, 2517, 2541, 2703, 3311
BmrI ACTGGG 1 cut(s) 1895
BmuI ACTGGG 1 cut(s) 1895
BpiI GAAGAC 2 cut(s) 1104, 1380
BplI GAGNNNNNCTC 2 cut(s) 947, 979
BpmI CTGGAG 3 cut(s) 430, 2778, 2868
Bpu10I CCTNAGC 1 cut(s) 3292
Bpu14I TTCGAA 2 cut(s) 190, 233
BpuEI CTTGAG 1 cut(s) 1763
BpuMI CCSGG 2 cut(s) 2703, 3311
BsaAI YACGTR 1 cut(s) 1864
BsaBI GATNNNNATC 3 cut(s) 987, 1221, 3305
BsaXI ACNNNNNCTCC 4 cut(s) 1426, 1456, 2580, 2610
Bse1I ACTGG 8 cut(s) 413, 807, 904, 1044, 1890, 2250, 2389, 2885
Bse3DI GCAATG 5 cut(s) 1061, 1518, 1911, 2044, 3411
Bse8I GATNNNNATC 3 cut(s) 987, 1221, 3305
BseBI CCWGG 5 cut(s) 787, 1418, 2238, 2517, 2541
BseJI GATNNNNATC 3 cut(s) 987, 1221, 3305
BseMI GCAATG 5 cut(s) 1061, 1518, 1911, 2044, 3411
BseMII CTCAG 9 cut(s) 161, 660, 751, 957, 1341, 2116, 2927, 3020, 3306
BseNI ACTGG 8 cut(s) 413, 807, 904, 1044, 1890, 2250, 2389, 2885
BseRI GAGGAG 6 cut(s) 167, 447, 2853, 2928, 2931, 2946
BseSI GKGCMC 1 cut(s) 2892
BseYI CCCAGC 2 cut(s) 1687, 3226
BsgI GTGCAG 6 cut(s) 23, 487, 1770, 2169, 2181, 2250
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 6 cut(s) 1283, 1967, 2137, 2155, 2626, 3127
BshNI GGYRCC 3 cut(s) 520, 1898, 2411
BsiHKAI GWGCWC 3 cut(s) 264, 576, 736
BsiHKCI CYCGRG 3 cut(s) 7, 263, 1830
BsiSI CCGG 3 cut(s) 257, 2703, 3311
BslFI GGGAC 3 cut(s) 1415, 2570, 2589
BsmAI GTCTC 4 cut(s) 150, 174, 1970, 3413
BsmBI CGTCTC 1 cut(s) 174
BsmFI GGGAC 3 cut(s) 1415, 2570, 2589
BsmI GAATGC 4 cut(s) 2530, 2547, 2737, 3106
BsnI GGCC 6 cut(s) 1283, 1967, 2137, 2155, 2626, 3127
BsoBI CYCGRG 3 cut(s) 7, 263, 1830
Bsp119I TTCGAA 2 cut(s) 190, 233
Bsp1286I GDGCHC 4 cut(s) 264, 576, 736, 2892
Bsp1407I TGTACA 2 cut(s) 313, 2347
Bsp19I CCATGG 2 cut(s) 1653, 2298
BspANI GGCC 6 cut(s) 1283, 1967, 2137, 2155, 2626, 3127
BspCNI CTCAG 9 cut(s) 160, 661, 750, 958, 1342, 2115, 2926, 3019, 3305
BspFNI CGCG 1 cut(s) 118
BspHI TCATGA 2 cut(s) 1533, 2284
BspMAI CTGCAG 3 cut(s) 382, 559, 3238
BspMI ACCTGC 2 cut(s) 961, 1374
BspT104I TTCGAA 2 cut(s) 190, 233
BspT107I GGYRCC 3 cut(s) 520, 1898, 2411
BsrDI GCAATG 5 cut(s) 1061, 1518, 1911, 2044, 3411
BsrGI TGTACA 2 cut(s) 313, 2347
BsrI ACTGG 8 cut(s) 413, 807, 904, 1044, 1890, 2250, 2389, 2885
BssNAI GTATAC 1 cut(s) 199
BssSI CACGAG 1 cut(s) 2481
BssT1I CCWWGG 7 cut(s) 1259, 1397, 1653, 1791, 1856, 1968, 2298
Bst1107I GTATAC 1 cut(s) 199
Bst2BI CACGAG 1 cut(s) 2481
Bst2UI CCWGG 5 cut(s) 787, 1418, 2238, 2517, 2541
Bst4CI ACNGT 7 cut(s) 637, 1296, 2271, 2347, 2844, 3053, 3058
BstAPI GCANNNNNTGC 1 cut(s) 2195
BstAUI TGTACA 2 cut(s) 313, 2347
BstBAI YACGTR 1 cut(s) 1864
BstBI TTCGAA 2 cut(s) 190, 233
BstC8I GCNNGC 6 cut(s) 270, 863, 2157, 2307, 3129, 3238
BstDSI CCRYGG 5 cut(s) 116, 1653, 2298, 2391, 2660
BstENI CCTNNNNNAGG 1 cut(s) 2460
BstFNI CGCG 1 cut(s) 118
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 2 cut(s) 131, 3067
BstMAI GTCTC 4 cut(s) 150, 174, 1970, 3413
BstNI CCWGG 5 cut(s) 787, 1418, 2238, 2517, 2541
BstNSI RCATGY 2 cut(s) 2248, 3170
BstSCI CCNGG 7 cut(s) 785, 1416, 2236, 2515, 2539, 2701, 3309
BstSFI CTRYAG 5 cut(s) 378, 555, 1285, 3117, 3234
BstSLI GKGCMC 1 cut(s) 2892
BstUI CGCG 1 cut(s) 118
BstV2I GAAGAC 2 cut(s) 1104, 1380
BstX2I RGATCY 8 cut(s) 1054, 1439, 1538, 1700, 2192, 2452, 2656, 3306
BstXI CCANNNNNNTGG 1 cut(s) 2183
BstYI RGATCY 8 cut(s) 1054, 1439, 1538, 1700, 2192, 2452, 2656, 3306
BstZ17I GTATAC 1 cut(s) 199
BsuRI GGCC 6 cut(s) 1283, 1967, 2137, 2155, 2626, 3127
BtgI CCRYGG 5 cut(s) 116, 1653, 2298, 2391, 2660
BtgZI GCGATG 2 cut(s) 51, 2096
BtsI GCAGTG 3 cut(s) 95, 288, 504
BtsIMutI CAGTG 8 cut(s) 95, 288, 504, 522, 814, 2025, 2892, 3020
BveI ACCTGC 2 cut(s) 961, 1374
Cac8I GCNNGC 6 cut(s) 270, 863, 2157, 2307, 3129, 3238
CaiI CAGNNNCTG 3 cut(s) 951, 1703, 3233
CciI TCATGA 2 cut(s) 1533, 2284
CfoI GCGC 2 cut(s) 131, 3067
Cfr13I GGNCC 6 cut(s) 1338, 1402, 1965, 2012, 2153, 2576
Cfr42I CCGCGG 1 cut(s) 119
CseI GACGC 1 cut(s) 1698
CsiI ACCWGGT 1 cut(s) 1416
Csp6I GTAC 9 cut(s) 306, 314, 921, 1801, 1899, 2348, 2825, 3054, 3153
CviQI GTAC 9 cut(s) 306, 314, 921, 1801, 1899, 2348, 2825, 3054, 3153
DraI TTTAAA 1 cut(s) 802
DraIII CACNNNGTG 2 cut(s) 2666, 2705
DriI GACNNNNNGTC 1 cut(s) 2582
Eam1105I GACNNNNNGTC 1 cut(s) 2582
EciI GGCGGA 1 cut(s) 1657
Ecl136II GAGCTC 1 cut(s) 262
Eco130I CCWWGG 7 cut(s) 1259, 1397, 1653, 1791, 1856, 1968, 2298
Eco147I AGGCCT 1 cut(s) 1283
Eco24I GRGCYC 1 cut(s) 264
Eco32I GATATC 1 cut(s) 884
Eco47I GGWCC 4 cut(s) 1338, 1402, 2012, 2576
Eco53kI GAGCTC 1 cut(s) 262
Eco57I CTGAAG 3 cut(s) 498, 1734, 2094
Eco72I CACGTG 1 cut(s) 1864
Eco88I CYCGRG 3 cut(s) 7, 263, 1830
EcoICRI GAGCTC 1 cut(s) 262
EcoNI CCTNNNNNAGG 1 cut(s) 2460
EcoO109I RGGNCCY 3 cut(s) 1402, 2012, 2576
EcoRII CCWGG 5 cut(s) 785, 1416, 2236, 2515, 2539
EcoRV GATATC 1 cut(s) 884
EcoT14I CCWWGG 7 cut(s) 1259, 1397, 1653, 1791, 1856, 1968, 2298
EcoT22I ATGCAT 1 cut(s) 1009
EcoT38I GRGCYC 1 cut(s) 264
ErhI CCWWGG 7 cut(s) 1259, 1397, 1653, 1791, 1856, 1968, 2298
Esp3I CGTCTC 1 cut(s) 174
FalI AAGNNNNNCTT 4 cut(s) 596, 628, 2635, 2667
FaqI GGGAC 3 cut(s) 1415, 2570, 2589
FauI CCCGC 1 cut(s) 120
FauNDI CATATG 2 cut(s) 1587, 2143
FbaI TGATCA 2 cut(s) 1192, 3300
FblI GTMKAC 1 cut(s) 198
FriOI GRGCYC 1 cut(s) 264
FspAI RTGCGCAY 1 cut(s) 3066
FspBI CTAG 9 cut(s) 107, 488, 939, 1260, 1578, 1893, 1926, 2316, 3186
FspI TGCGCA 1 cut(s) 3066
GlaI GCGC 2 cut(s) 130, 3066
GsaI CCCAGC 2 cut(s) 1691, 3230
GsuI CTGGAG 3 cut(s) 430, 2778, 2868
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 6 cut(s) 1283, 1967, 2137, 2155, 2626, 3127
HapII CCGG 3 cut(s) 257, 2703, 3311
HgaI GACGC 1 cut(s) 1698
HhaI GCGC 2 cut(s) 131, 3067
Hin6I GCGC 2 cut(s) 129, 3065
HinP1I GCGC 2 cut(s) 129, 3065
HincII GTYRAC 1 cut(s) 3177
HindII GTYRAC 1 cut(s) 3177
HindIII AAGCTT 2 cut(s) 270, 2420
HpaII CCGG 3 cut(s) 257, 2703, 3311
HphI GGTGA 9 cut(s) 568, 699, 914, 1419, 1518, 1587, 2436, 2691, 2943
Hpy166II GTNNAC 7 cut(s) 199, 314, 1292, 1341, 1847, 2632, 3177
Hpy8I GTNNAC 7 cut(s) 199, 314, 1292, 1341, 1847, 2632, 3177
Hpy99I CGWCG 1 cut(s) 1111
HpyAV CCTTC 4 cut(s) 589, 2036, 2466, 2758
HpyCH4III ACNGT 7 cut(s) 637, 1296, 2271, 2347, 2844, 3053, 3058
HpyCH4IV ACGT 1 cut(s) 1863
HpySE526I ACGT 1 cut(s) 1863
HspAI GCGC 2 cut(s) 129, 3065
KflI GGGWCCC 1 cut(s) 2576
KpnI GGTACC 1 cut(s) 1902
Ksp22I TGATCA 2 cut(s) 1192, 3300
KspI CCGCGG 1 cut(s) 119
MabI ACCWGGT 1 cut(s) 1416
MaeI CTAG 9 cut(s) 107, 488, 939, 1260, 1578, 1893, 1926, 2316, 3186
MaeII ACGT 1 cut(s) 1863
MaeIII GTNAC 9 cut(s) 705, 1374, 1432, 1593, 2023, 2049, 2126, 2320, 2764
MflI RGATCY 8 cut(s) 1054, 1439, 1538, 1700, 2192, 2452, 2656, 3306
MhlI GDGCHC 4 cut(s) 264, 576, 736, 2892
MlyI GAGTC 4 cut(s) 236, 1972, 2761, 3403
MmeI TCCRAC 3 cut(s) 386, 1052, 1924
Mph1103I ATGCAT 1 cut(s) 1009
MseI TTAA 7 cut(s) 714, 743, 801, 1125, 1134, 1158, 1955
MslI CAYNNNNRTG 5 cut(s) 996, 1938, 2396, 2730, 3360
MspA1I CMGCKG 1 cut(s) 118
MspI CCGG 3 cut(s) 257, 2703, 3311
MspR9I CCNGG 7 cut(s) 787, 1418, 2238, 2517, 2541, 2703, 3311
Mva1269I GAATGC 4 cut(s) 2530, 2547, 2737, 3106
MvaI CCWGG 5 cut(s) 787, 1418, 2238, 2517, 2541
MvnI CGCG 1 cut(s) 118
NciI CCSGG 2 cut(s) 2703, 3311
NcoI CCATGG 2 cut(s) 1653, 2298
NdeI CATATG 2 cut(s) 1587, 2143
NmuCI GTSAC 6 cut(s) 705, 1374, 1432, 1593, 2023, 2764
NsbI TGCGCA 1 cut(s) 3066
NsiI ATGCAT 1 cut(s) 1009
NspI RCATGY 2 cut(s) 2248, 3170
NspV TTCGAA 2 cut(s) 190, 233
PaeR7I CTCGAG 1 cut(s) 263
PagI TCATGA 2 cut(s) 1533, 2284
PceI AGGCCT 1 cut(s) 1283
PciI ACATGT 1 cut(s) 2244
PctI GAATGC 4 cut(s) 2530, 2547, 2737, 3106
PflMI CCANNNNNTGG 2 cut(s) 2144, 2299
PleI GAGTC 4 cut(s) 236, 1972, 2761, 3402
PmaCI CACGTG 1 cut(s) 1864
PmlI CACGTG 1 cut(s) 1864
PpsI GAGTC 4 cut(s) 236, 1972, 2761, 3402
Ppu21I YACGTR 1 cut(s) 1864
PpuMI RGGWCCY 3 cut(s) 1402, 2012, 2576
PscI ACATGT 1 cut(s) 2244
PsiI TTATAA 1 cut(s) 395
Psp124BI GAGCTC 1 cut(s) 264
Psp5II RGGWCCY 3 cut(s) 1402, 2012, 2576
Psp6I CCWGG 5 cut(s) 785, 1416, 2236, 2515, 2539
PspCI CACGTG 1 cut(s) 1864
PspFI CCCAGC 2 cut(s) 1687, 3226
PspGI CCWGG 5 cut(s) 785, 1416, 2236, 2515, 2539
PspPI GGNCC 6 cut(s) 1338, 1402, 1965, 2012, 2153, 2576
PspPPI RGGWCCY 3 cut(s) 1402, 2012, 2576
PspXI VCTCGAGB 1 cut(s) 263
PsrI GAACNNNNNNTAC 2 cut(s) 1882, 1914
PstI CTGCAG 3 cut(s) 382, 559, 3238
PstNI CAGNNNCTG 3 cut(s) 951, 1703, 3233
PsuI RGATCY 8 cut(s) 1054, 1439, 1538, 1700, 2192, 2452, 2656, 3306
RsaI GTAC 9 cut(s) 307, 315, 922, 1802, 1900, 2349, 2826, 3055, 3154
RsaNI GTAC 9 cut(s) 306, 314, 921, 1801, 1899, 2348, 2825, 3054, 3153
RseI CAYNNNNRTG 5 cut(s) 996, 1938, 2396, 2730, 3360
SacI GAGCTC 1 cut(s) 264
SacII CCGCGG 1 cut(s) 119
SaqAI TTAA 7 cut(s) 714, 743, 801, 1125, 1134, 1158, 1955
Sau96I GGNCC 6 cut(s) 1338, 1402, 1965, 2012, 2153, 2576
SchI GAGTC 4 cut(s) 236, 1972, 2761, 3403
ScrFI CCNGG 7 cut(s) 787, 1418, 2238, 2517, 2541, 2703, 3311
SduI GDGCHC 4 cut(s) 264, 576, 736, 2892
SexAI ACCWGGT 1 cut(s) 1416
SfcI CTRYAG 5 cut(s) 378, 555, 1285, 3117, 3234
Sfr274I CTCGAG 1 cut(s) 263
Sfr303I CCGCGG 1 cut(s) 119
SfuI TTCGAA 2 cut(s) 190, 233
SgrBI CCGCGG 1 cut(s) 119
SinI GGWCC 4 cut(s) 1338, 1402, 2012, 2576
SlaI CTCGAG 1 cut(s) 263
SmiMI CAYNNNNRTG 5 cut(s) 996, 1938, 2396, 2730, 3360
SmlI CTYRAG 2 cut(s) 263, 1778
SmoI CTYRAG 2 cut(s) 263, 1778
SseBI AGGCCT 1 cut(s) 1283
SspI AATATT 2 cut(s) 3095, 3423
SspMI CTAG 9 cut(s) 107, 488, 939, 1260, 1578, 1893, 1926, 2316, 3186
SstI GAGCTC 1 cut(s) 264
StuI AGGCCT 1 cut(s) 1283
StyD4I CCNGG 7 cut(s) 785, 1416, 2236, 2515, 2539, 2701, 3309
StyI CCWWGG 7 cut(s) 1259, 1397, 1653, 1791, 1856, 1968, 2298
TaaI ACNGT 7 cut(s) 637, 1296, 2271, 2347, 2844, 3053, 3058
TaiI ACGT 1 cut(s) 1866
TaqI TCGA 4 cut(s) 190, 233, 264, 3444
TatI WGTACW 5 cut(s) 313, 920, 1800, 2347, 3053
TauI GCSGC 3 cut(s) 73, 118, 2926
Tru1I TTAA 7 cut(s) 714, 743, 801, 1125, 1134, 1158, 1955
Tru9I TTAA 7 cut(s) 714, 743, 801, 1125, 1134, 1158, 1955
TscAI CASTG 8 cut(s) 102, 295, 511, 522, 814, 2032, 2892, 3020
TseFI GTSAC 6 cut(s) 705, 1374, 1432, 1593, 2023, 2764
Tsp45I GTSAC 6 cut(s) 705, 1374, 1432, 1593, 2023, 2764
TspGWI ACGGA 4 cut(s) 184, 523, 1047, 2408
TspRI CASTG 8 cut(s) 102, 295, 511, 522, 814, 2032, 2892, 3020
Van91I CCANNNNNTGG 2 cut(s) 2144, 2299
VpaK11BI GGWCC 4 cut(s) 1338, 1402, 2012, 2576
XagI CCTNNNNNAGG 1 cut(s) 2460
XapI RAATTY 4 cut(s) 662, 1450, 2060, 2601
XceI RCATGY 2 cut(s) 2248, 3170
XcmI CCANNNNNNNNNTGG 2 cut(s) 1940, 2649
XhoI CTCGAG 1 cut(s) 263
XmaJI CCTAGG 1 cut(s) 1259
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 9 cut(s) 107, 488, 939, 1260, 1578, 1893, 1926, 2316, 3186
Zsp2I ATGCAT 1 cut(s) 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.