AT1G28050

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009189
Physical Location & Seq
Reverse (-)
9775522 .. 9778043
2522 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G28050.1

Sequence Viewer

Length: 1302 bp
ATGAGTAGTTCGGAGAGAGTACCGTGCGATTTCTGCGGCGAGCGTACGGCGGTTTTGTTTTGTAGAGCCGATACGGCGAAGCTGTGTTTGCCTTGTGATCAGCAAGTTCACACGGCGAATCTGTTGTCGAGGAAGCACGTGCGATCTCAGATCTGCGATAATTGCGGTAACGAGCCAGTCTCTGTTCGGTGTTTCACCGATAATCTGATTTTGTGTCAGGAGTGTGATTGGGATGTTCACGGAAGTTGTTCAGTTTCCGATGCTCATGTTCGATCCGCCGTGGAAGGTTTTTCCGGTTGTCCATCGGCGTTGGAGCTTGCTGCTTTATGGGGACTTGATTTGGAGCAAGGGAGGAAAGATGAAGAGAATCAAGTTCCGATGATGGCGATGATGATGGATAATTTCGGGATGCAGTTGGATTCTTGGGTTTTGGGATCTAATGAATTGATTGTTCCCAGCGATACGACGTTTAAGAAGCGTGGATCTTGTGGATCTAGTTGTGGGAGGTATAAGCAGGTATTGTGTAAGCAGCTTGAGGAGTTGCTTAAGAGTGGTGTTGTCGGTGGTGATGGCGATGATGGTGATCGTGACCGTGATTGTGACCGTGAGGGTGCTTGTGATGGAGATGGAGATGGAGAAGCAGGAGAGGGGCTTATGGTTCCGGAGATGTCAGAGAGATTGAAATGGTCAAGAGATGTTGAGGAGATCAATGGTGGCGGAGGAGGAGGAGTTAACCAGCAGTGGAATGCTACTACTACTAATCCTAGTGGTGGCCAGAGTTCTCAGATATGGGATTTTAACTTGGGACAGTCACGGGGACCTGAGGATACGAGTCGAGTGGAAGCTGCATATGTAGGGAAAGGTGCTGCTTCTTCATTCACAATCAACAATTTTGTTGACCATATGAATGAAACTTGTTCCACTAATGTGAAAGGTGTCAAAGAGATTAAAAAGGATGACTACAAGCGATCAACTTCAGGCCAGGTACAACCAACAAAATCTGAGAGCAACAATCGTCCAATTACCTTTGGCTCTGAGAAAGGTTCGAACTCCTCCAGTGACTTGCATTTCACAGAGCATATTGCTGGAACTAGTTGTAAGACCACAAGACTAGTTGCAACTAAGGCTGATCTGGAGCGGCTGGCTCAGAACAGAGGAGATGCAATGCAGCGTTACAAGGAAAAGAGGAAGACACGGAGATATGATAAGACCATAAGGTATGAATCGAGGAAGGCAAGAGCTGACACTAGGTTGCGTGTCAGAGGCAGATTTGTGAAAGCTAGTGAAGCTCCTTACCCTTAA

Protein Analysis

433

Amino Acids

47.61

Weight (kDa)

5.8

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCT PF06203 385 - 427 8.5e-17 CCT motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 505
AccBSI CCGCTC 1 cut(s) 1138
AccIII TCCGGA 1 cut(s) 661
AciI CCGC 6 cut(s) 36, 50, 165, 276, 717, 1138
AclWI GGATC 4 cut(s) 267, 442, 490, 499
AcoI YGGCCR 1 cut(s) 772
AcuI CTGAAG 1 cut(s) 960
AcvI CACGTG 1 cut(s) 139
AfaI GTAC 3 cut(s) 21, 46, 987
AfiI CCNNNNNNNGG 1 cut(s) 770
AflII CTTAAG 1 cut(s) 545
AgsI TTSAA 1 cut(s) 682
AhlI ACTAGT 2 cut(s) 1091, 1111
AjnI CCWGG 1 cut(s) 981
AleI CACNNNNGTG 1 cut(s) 926
AluBI AGCT 7 cut(s) 82, 316, 532, 845, 1241, 1280, 1289
AluI AGCT 7 cut(s) 82, 316, 532, 845, 1241, 1280, 1289
Alw26I GTCTC 1 cut(s) 184
AlwI GGATC 4 cut(s) 267, 442, 490, 499
AlwNI CAGNNNCTG 1 cut(s) 182
Aor13HI TCCGGA 1 cut(s) 661
AoxI GGCC 2 cut(s) 772, 979
ApeKI GCWGC 5 cut(s) 320, 529, 845, 866, 1168
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
Asp700I GAANNNNTTC 1 cut(s) 247
AspS9I GGNCC 1 cut(s) 818
AsuHPI GGTGA 3 cut(s) 187, 578, 593
AsuII TTCGAA 1 cut(s) 1046
AvaII GGWCC 1 cut(s) 818
AxyI CCTNAGG 1 cut(s) 822
BalI TGGCCA 1 cut(s) 774
BbrPI CACGTG 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 1196
BbvI GCAGC 5 cut(s) 307, 541, 832, 853, 1180
BccI CCATC 8 cut(s) 310, 376, 388, 563, 572, 614, 620, 626
BceAI ACGGC 4 cut(s) 63, 90, 129, 263
BciT130I CCWGG 1 cut(s) 983
BciVI GTATCC 1 cut(s) 820
BclI TGATCA 1 cut(s) 97
BcoDI GTCTC 1 cut(s) 184
BcuI ACTAGT 2 cut(s) 1091, 1111
BfaI CTAG 6 cut(s) 495, 765, 1092, 1112, 1248, 1281
BfrI CTTAAG 1 cut(s) 545
BfuAI ACCTGC 1 cut(s) 505
BfuI GTATCC 1 cut(s) 820
BglI GCCNNNNNGGC 1 cut(s) 74
BglII AGATCT 1 cut(s) 150
BisI GCNGC 7 cut(s) 37, 321, 530, 846, 867, 1139, 1169
BlsI GCNGC 7 cut(s) 38, 322, 531, 847, 868, 1140, 1170
Bme1390I CCNGG 1 cut(s) 983
Bme18I GGWCC 1 cut(s) 818
BmgT120I GGNCC 1 cut(s) 818
BmiI GGNNCC 2 cut(s) 660, 819
BmrFI CCNGG 1 cut(s) 983
BmsI GCATC 3 cut(s) 250, 399, 1150
BpiI GAAGAC 1 cut(s) 1196
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BpmI CTGGAG 2 cut(s) 1039, 1154
Bpu14I TTCGAA 1 cut(s) 1046
BpuEI CTTGAG 1 cut(s) 554
BsaAI YACGTR 1 cut(s) 139
BsaBI GATNNNNATC 1 cut(s) 582
BsaJI CCNNGG 1 cut(s) 279
BsaWI WCCGGW 2 cut(s) 293, 661
BsaXI ACNNNNNCTCC 2 cut(s) 714, 744
Bsc4I CCNNNNNNNGG 1 cut(s) 770
Bse1I ACTGG 2 cut(s) 176, 1056
Bse21I CCTNAGG 1 cut(s) 822
Bse3DI GCAATG 1 cut(s) 1170
Bse8I GATNNNNATC 1 cut(s) 582
BseAI TCCGGA 1 cut(s) 661
BseBI CCWGG 1 cut(s) 983
BseDI CCNNGG 1 cut(s) 279
BseGI GGATG 3 cut(s) 238, 414, 961
BseJI GATNNNNATC 1 cut(s) 582
BseLI CCNNNNNNNGG 1 cut(s) 770
BseMI GCAATG 1 cut(s) 1170
BseMII CTCAG 6 cut(s) 161, 797, 813, 993, 1026, 1160
BseNI ACTGG 2 cut(s) 176, 1056
BseRI GAGGAG 7 cut(s) 551, 716, 735, 738, 741, 1042, 1170
BseXI GCAGC 5 cut(s) 307, 541, 832, 853, 1180
BseYI CCCAGC 1 cut(s) 455
BshFI GGCC 2 cut(s) 774, 981
BsiSI CCGG 2 cut(s) 294, 662
BsiWI CGTACG 1 cut(s) 44
BslFI GGGAC 3 cut(s) 345, 819, 831
BslI CCNNNNNNNGG 1 cut(s) 770
BsmAI GTCTC 1 cut(s) 184
BsmFI GGGAC 3 cut(s) 345, 819, 831
BsmI GAATGC 1 cut(s) 751
BsnI GGCC 2 cut(s) 774, 981
Bsp119I TTCGAA 1 cut(s) 1046
Bsp13I TCCGGA 1 cut(s) 661
BspACI CCGC 6 cut(s) 36, 50, 165, 276, 717, 1138
BspANI GGCC 2 cut(s) 774, 981
BspCNI CTCAG 6 cut(s) 160, 796, 814, 994, 1027, 1159
BspEI TCCGGA 1 cut(s) 661
BspLI GGNNCC 2 cut(s) 660, 819
BspMI ACCTGC 1 cut(s) 505
BspPI GGATC 4 cut(s) 267, 442, 490, 499
BspT104I TTCGAA 1 cut(s) 1046
BspTI CTTAAG 1 cut(s) 545
BsrBI CCGCTC 1 cut(s) 1138
BsrDI GCAATG 1 cut(s) 1170
BsrI ACTGG 2 cut(s) 176, 1056
BssECI CCNNGG 1 cut(s) 279
Bst2UI CCWGG 1 cut(s) 983
Bst4CI ACNGT 4 cut(s) 24, 593, 605, 810
Bst6I CTCTTC 1 cut(s) 357
BstAFI CTTAAG 1 cut(s) 545
BstBAI YACGTR 1 cut(s) 139
BstBI TTCGAA 1 cut(s) 1046
BstC8I GCNNGC 3 cut(s) 41, 318, 1143
BstDEI CTNAG 7 cut(s) 147, 783, 822, 1002, 1035, 1122, 1146
BstDSI CCRYGG 1 cut(s) 279
BstF5I GGATG 3 cut(s) 238, 414, 961
BstMAI GTCTC 1 cut(s) 184
BstMWI GCNNNNNNNGC 6 cut(s) 33, 74, 88, 162, 1124, 1286
BstNI CCWGG 1 cut(s) 983
BstSCI CCNGG 1 cut(s) 981
BstV1I GCAGC 5 cut(s) 307, 541, 832, 853, 1180
BstV2I GAAGAC 1 cut(s) 1196
BstX2I RGATCY 4 cut(s) 150, 434, 482, 491
BstYI RGATCY 4 cut(s) 150, 434, 482, 491
Bsu36I CCTNAGG 1 cut(s) 822
BsuI GTATCC 1 cut(s) 820
BsuRI GGCC 2 cut(s) 774, 981
BtgI CCRYGG 1 cut(s) 279
BtgZI GCGATG 2 cut(s) 401, 588
BtsCI GGATG 3 cut(s) 238, 414, 961
BtsI GCAGTG 1 cut(s) 746
BtsIMutI CAGTG 2 cut(s) 746, 1063
BveI ACCTGC 1 cut(s) 505
Cac8I GCNNGC 3 cut(s) 41, 318, 1143
CaiI CAGNNNCTG 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 818
Csp6I GTAC 3 cut(s) 20, 45, 986
CviAII CATG 1 cut(s) 266
CviQI GTAC 3 cut(s) 20, 45, 986
DdeI CTNAG 7 cut(s) 147, 783, 822, 1002, 1035, 1122, 1146
EaeI YGGCCR 1 cut(s) 772
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
EciI GGCGGA 2 cut(s) 265, 732
Eco47I GGWCC 1 cut(s) 818
Eco57I CTGAAG 1 cut(s) 960
Eco72I CACGTG 1 cut(s) 139
Eco81I CCTNAGG 1 cut(s) 822
EcoO109I RGGNCCY 1 cut(s) 818
EcoRII CCWGG 1 cut(s) 981
FaeI CATG 1 cut(s) 269
FaqI GGGAC 3 cut(s) 345, 819, 831
FatI CATG 1 cut(s) 265
FauNDI CATATG 2 cut(s) 850, 903
FbaI TGATCA 1 cut(s) 97
Fnu4HI GCNGC 7 cut(s) 37, 321, 530, 846, 867, 1139, 1169
FokI GGATG 3 cut(s) 245, 421, 968
Fsp4HI GCNGC 7 cut(s) 37, 321, 530, 846, 867, 1139, 1169
FspBI CTAG 6 cut(s) 495, 765, 1092, 1112, 1248, 1281
GluI GCNGC 7 cut(s) 37, 321, 530, 846, 867, 1139, 1169
GsaI CCCAGC 1 cut(s) 459
GsuI CTGGAG 2 cut(s) 1039, 1154
HaeIII GGCC 2 cut(s) 774, 981
HapII CCGG 2 cut(s) 294, 662
Hin1II CATG 1 cut(s) 269
HincII GTYRAC 2 cut(s) 733, 898
HindII GTYRAC 2 cut(s) 733, 898
HinfI GANTC 5 cut(s) 118, 367, 419, 832, 1223
HpaI GTTAAC 1 cut(s) 733
HpaII CCGG 2 cut(s) 294, 662
HphI GGTGA 3 cut(s) 187, 578, 593
Hpy166II GTNNAC 4 cut(s) 109, 238, 733, 898
Hpy188III TCNNGA 6 cut(s) 218, 406, 587, 662, 690, 1133
Hpy8I GTNNAC 4 cut(s) 109, 238, 733, 898
Hpy99I CGWCG 1 cut(s) 469
HpyAV CCTTC 2 cut(s) 278, 1225
HpyCH4III ACNGT 4 cut(s) 24, 593, 605, 810
HpyCH4IV ACGT 2 cut(s) 138, 467
HpyCH4V TGCA 6 cut(s) 412, 848, 1066, 1118, 1163, 1168
HpyF10VI GCNNNNNNNGC 6 cut(s) 33, 74, 88, 162, 1124, 1286
HpyF3I CTNAG 7 cut(s) 147, 783, 822, 1002, 1035, 1122, 1146
HpySE526I ACGT 2 cut(s) 138, 467
Hsp92II CATG 1 cut(s) 269
Kpn2I TCCGGA 1 cut(s) 661
Ksp22I TGATCA 1 cut(s) 97
KspAI GTTAAC 1 cut(s) 733
LmnI GCTCC 4 cut(s) 313, 343, 1135, 1294
Lsp1109I GCAGC 5 cut(s) 307, 541, 832, 853, 1180
LweI GCATC 3 cut(s) 250, 399, 1150
MaeI CTAG 6 cut(s) 495, 765, 1092, 1112, 1248, 1281
MaeII ACGT 2 cut(s) 138, 467
MaeIII GTNAC 6 cut(s) 167, 587, 599, 810, 1058, 1172
MbiI CCGCTC 1 cut(s) 1138
MboII GAAGA 3 cut(s) 374, 864, 1201
MflI RGATCY 4 cut(s) 150, 434, 482, 491
MlsI TGGCCA 1 cut(s) 774
MluCI AATT 5 cut(s) 160, 400, 443, 889, 1020
MluNI TGGCCA 1 cut(s) 774
MlyI GAGTC 1 cut(s) 841
MmeI TCCRAC 2 cut(s) 291, 396
Mox20I TGGCCA 1 cut(s) 774
MroI TCCGGA 1 cut(s) 661
MroXI GAANNNNTTC 1 cut(s) 247
MscI TGGCCA 1 cut(s) 774
MseI TTAA 6 cut(s) 471, 546, 732, 798, 948, 1300
MslI CAYNNNNRTG 2 cut(s) 906, 926
Msp20I TGGCCA 1 cut(s) 774
MspCI CTTAAG 1 cut(s) 545
MspI CCGG 2 cut(s) 294, 662
MspR9I CCNGG 1 cut(s) 983
Mva1269I GAATGC 1 cut(s) 751
MvaI CCWGG 1 cut(s) 983
MwoI GCNNNNNNNGC 6 cut(s) 33, 74, 88, 162, 1124, 1286
NdeI CATATG 2 cut(s) 850, 903
NlaIII CATG 1 cut(s) 269
NlaIV GGNNCC 2 cut(s) 660, 819
NmuCI GTSAC 4 cut(s) 587, 599, 810, 1058
NspV TTCGAA 1 cut(s) 1046
OliI CACNNNNGTG 1 cut(s) 926
PctI GAATGC 1 cut(s) 751
PdmI GAANNNNTTC 1 cut(s) 247
PfeI GAWTC 4 cut(s) 118, 367, 419, 1223
Pfl23II CGTACG 1 cut(s) 44
PkrI GCNGC 7 cut(s) 38, 322, 531, 847, 868, 1140, 1170
PleI GAGTC 1 cut(s) 840
PmaCI CACGTG 1 cut(s) 139
PmlI CACGTG 1 cut(s) 139
PpsI GAGTC 1 cut(s) 840
Ppu21I YACGTR 1 cut(s) 139
PpuMI RGGWCCY 1 cut(s) 818
Psp5II RGGWCCY 1 cut(s) 818
Psp6I CCWGG 1 cut(s) 981
PspCI CACGTG 1 cut(s) 139
PspFI CCCAGC 1 cut(s) 455
PspGI CCWGG 1 cut(s) 981
PspLI CGTACG 1 cut(s) 44
PspN4I GGNNCC 2 cut(s) 660, 819
PspPI GGNCC 1 cut(s) 818
PspPPI RGGWCCY 1 cut(s) 818
PstNI CAGNNNCTG 1 cut(s) 182
PsuI RGATCY 4 cut(s) 150, 434, 482, 491
RsaI GTAC 3 cut(s) 21, 46, 987
RsaNI GTAC 3 cut(s) 20, 45, 986
RseI CAYNNNNRTG 2 cut(s) 906, 926
SaqAI TTAA 6 cut(s) 471, 546, 732, 798, 948, 1300
SatI GCNGC 7 cut(s) 37, 321, 530, 846, 867, 1139, 1169
Sau96I GGNCC 1 cut(s) 818
SchI GAGTC 1 cut(s) 841
ScrFI CCNGG 1 cut(s) 983
SfaNI GCATC 3 cut(s) 250, 399, 1150
SfuI TTCGAA 1 cut(s) 1046
SinI GGWCC 1 cut(s) 818
SmiMI CAYNNNNRTG 2 cut(s) 906, 926
SmlI CTYRAG 2 cut(s) 533, 545
SmoI CTYRAG 2 cut(s) 533, 545
SpeI ACTAGT 2 cut(s) 1091, 1111
Sse9I AATT 5 cut(s) 160, 400, 443, 889, 1020
SsiI CCGC 6 cut(s) 36, 50, 165, 276, 717, 1138
SspMI CTAG 6 cut(s) 495, 765, 1092, 1112, 1248, 1281
StyD4I CCNGG 1 cut(s) 981
TaaI ACNGT 4 cut(s) 24, 593, 605, 810
TaiI ACGT 2 cut(s) 141, 470
TaqI TCGA 5 cut(s) 128, 271, 835, 1046, 1226
TasI AATT 5 cut(s) 160, 400, 443, 889, 1020
TauI GCSGC 2 cut(s) 39, 1141
TfiI GAWTC 4 cut(s) 118, 367, 419, 1223
Tru1I TTAA 6 cut(s) 471, 546, 732, 798, 948, 1300
Tru9I TTAA 6 cut(s) 471, 546, 732, 798, 948, 1300
TscAI CASTG 2 cut(s) 746, 1063
TseFI GTSAC 4 cut(s) 587, 599, 810, 1058
TseI GCWGC 5 cut(s) 320, 529, 845, 866, 1168
Tsp45I GTSAC 4 cut(s) 587, 599, 810, 1058
TspDTI ATGAA 6 cut(s) 375, 456, 864, 920, 924, 1236
TspGWI ACGGA 2 cut(s) 255, 1210
TspRI CASTG 2 cut(s) 746, 1063
Vha464I CTTAAG 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 818
XmnI GAANNNNTTC 1 cut(s) 247
XspI CTAG 6 cut(s) 495, 765, 1092, 1112, 1248, 1281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.