Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006461

Physical Location & Seq

Genomic Coordinates Forward (+)

10727317 .. 10738092

Gene Structure 10776 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G30400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4869 bp

ATGGGGTTTGAGCCGTTGGATTGGTATTGCAAGCCGGTGCCGAATGGTGTGTGGACTAAAACTGTGGATTATGCGTTTGGTGCATACACGCCTTGTGCTATTGACTCTTTTGTGCTTGGTATCTCTCATCTGGTTCTGTTGATTCTGTGTCTTTATCGCTTGTGGCTCATCACGAAGGATCACAAAGTGGATAAGTTCTGCTTGAGGTCTAAATGGTTTAGCTATTTTCTGGCTCTTTTGGCTGCTTATGCTACTGCGGAGCCTTTGTTTAGATTGGTCATGAGGATCTCTGTTTTGGATTTGGATGGAGCTGGGTTTCCTCCCTATGAGGCGTTTATGTTGGTCCTTGAGGCTTTTGCTTGGGGTTCTGCTTTGGTCATGACTGTTGTGGAAACTAAAACGTATATCCATGAACTCCGTTGGTATGTCAGATTCGCTGTCATTTATGCTCTTGTGGGAGACATGGTGTTGTTAAATCTTGTTCTCTCTGTTAAGGAGTACTATGGCAGTTTTAAACTGTATCTTTACATAAGCGAGGTGGCAGTTCAGGTTGCATTTGGAACCCTCTTGTTTGTGTATTTCCCTAATTTGGACCCTTACCCTGGTTACACACCAGTTGGGACTGAAAATTCCGAGGATTACGAGTATGAAGAGCTTCCTGGAGGAGAAAATATATGTCCTGAGAGGCATGCAAATTTATTTGACAGTATCTTCTTCTCATGGTTGAACCCATTGATGACTCTGGGATCAAAACGACCTCTCACCGAGAAGGATGTATGGCATCTGGACACTTGGGATAAAACTGAAACTCTTATGAGGAGCTTCCAGAAGTCCTGGGATAAGGAACTAGAAAAGCCCAAACCGTGGCTTTTGAGAGCACTGAACAACAGCCTTGGGGGAAGGTTTTGGTGGGGTGGCTTTTGGAAGATTGGGAATGACTGTTCACAGTTCGTGGGGCCTCTTCTACTGAATGAGCTCTTAAAGTCAATGCAACTTAATGAACCAGCGTGGATAGGTTACATCTATGCAATCTCAATCTTTGTTGGAGTGGTATTGGGGGTTTTATGTGAAGCTCAGTATTTCCAAAATGTGATGCGTGTTGGTTACCGGCTTAGGTCTGCACTGATTGCTGCTGTGTTCCGAAAATCTTTGAGGCTAACTAATGAGGGGCGGAAGAAGTTTCAAACAGGAAAAATAACAAACTTAATGACTACTGATGCTGAGTCGCTGCAGCAAATCTGCCAATCACTTCATACCATGTGGTCGGCGCCATTTCGTATAATTGTAGCACTGGTTCTCCTCTATCAACAATTGGGTGTTGCCTCGATCATTGGTGCATTGTTTCTTGTCCTTATGTTCCCCATACAGACTGTTATTATAAGCAAAACGCAGAAGTTAACAAAAGAAGGGTTGCAGCGTACTGACAAGAGAATTGGCCTAATGAATGAGGTTTTAGCGGCAATGGATACAGTGAAGTGTTACGCTTGGGAAAACAGTTTTCAGTCCAAGGTTCAAACTGTACGTGATGATGAATTATCTTGGTTCCGGAAAGCACAACTCCTGTCAGCGTTCAATATGTTCATACTAAACAGCATTCCTGTCCTCGTGACTGTTGTTTCATTTGGTGTGTTCTCATTGCTTGGAGGAGATCTGACACCTGCAAGAGCGTTTACGTCACTCTCTCTATTTTCTGTGCTTCGCTTCCCTTTATTCATGCTTCCAAACATTATAACTCAGATGGTAAATGCTAATGTATCCTTAAACCGTTTGGAGGAGGTACTGTCAACCGAAGAGAGAGTTCTCTTACCGAATCCTCCCATTGAACCTGGACAGCCAGCTATCTCAATAAGAAATGGATACTTCTCCTGGGATTCAAAGGCGGATAGGCCAACATTGTCAAACATCAACCTGGACATACCTCTTGGCAGCCTAGTTGCGGTAGTTGGCAGCACAGGAGAAGGAAAAACCTCCCTGATATCTGCTATGCTTGGGGAACTTCCTGCAAGATCTGATGCGACTGTTACTCTTAGAGGATCAGTCGCTTATGTTCCACAAGTTTCATGGATCTTTAACGCAACAGTACGTGACAATATATTGTTTGGGGCTCCTTTTGACCAAGAAAAATATGAAAGGGTGATTGATGTGACAGCACTCCAGCATGACCTTGAGTTACTGCCTGGAGGTGACCTCACGGAGATCGGAGAAAGGGGTGTTAACATCAGTGGGGGACAAAAGCAGAGGGTTTCTATGGCTAGGGCCGTTTACTCAAATTCAGACGTGTGCATCTTAGATGACCCATTGAGTGCCCTTGATGCGCATGTTGGTCAGCAGGTTTTTGAAAAATGCATAAAAAGGGAACTAGGGCAGACAACGAGAGTACTTGTTACAAATCAGCTCCACTTCCTATCACAAGTGGATAAAATCCTACTTGTCCATGAGGGAACAGTAAAAGAGGAAGGAACATATGAAGAATTATGCCATAGTGGCCCGTTGTTCCAGAGGTTAATGGAAAATGCAGGGAAGGTTGAAGATTATTCAGAAGAAAATGGAGAAGCTGAAGTAGATCAAACATCTGTAAAACCAGTTGAAAATGGGAACGCTAATAATCTGCAGAAGGATGGAATCGAGACAAAGAATTCCAAAGAAGGAAACTCTGTTCTTGTCAAGCGAGAAGAACGTGAAACTGGAGTTGTGAGTTGGAAAGTCCTGGAGAGGTACCAGAATGCACTTGGAGGTGCATGGGTAGTGATGATGCTCGTTATATGCTACGTCTTGACTCAAGTATTTCGGGTTTCAAGCAGCACTTGGTTGAGTGAGTGGACTGATTCAGGAACCCCAAAGACTCATGGACCCCTATTCTATAATATTGTCTATGCGCTTCTTTCGTTTGGACAGGTCTCTGTGACATTGATCAATTCATATTGGTTGATTATGTCCAGTCTATATGCAGCTAAAAAGATGCATGATGCTATGCTTGGTTCCATACTAAGGGCTCCAATGGTGTTCTTTCAAACCAATCCATTAGGACGGATAATCAATCGATTTGCAAAAGATATGGGAGATATTGATCGAACTGTGGCAGTCTTTGTAAACATGTTTATGGGTTCAATCGCACAGCTTCTTTCAACTGTTATCTTGATTGGCATTGTCAGCACTCTGTCCCTGTGGGCCATCATGCCCCTGTTGGTCGTGTTCTATGGAGCTTATCTGTATTACCAGAACACATCTCGGGAAATTAAACGTATGGATTCCACTACAAGATCGCCAGTTTATGCTCAATTTGGTGAGGCATTGAATGGACTATCTAGTATCCGTGCTTATAAAGCATATGACAGGATGGCTGAAATTAATGGAAGGTCAATGGACAATAACATCAGATTCACACTTGTAAACATGGCTGCAAATCGGTGGCTGGGAATCCGTTTGGAAGTTTTGGGAGGTCTCATGGTTTGGTTGACTGCGTCATTAGCCGTCATGCAGAACGGAAAGGCAGCGAACCAACAAGCATATGCATCTACGATGGGTTTGCTTCTCAGTTATGCGTTAAGCATTACCAGCTCTTTAACAGCTGTACTGAGACTCGCGAGTCTAGCTGAGAATAGTTTAAACTCGGTTGAGCGTGTTGGAAATTATATCGAGATACCATCAGAGGCTCCATTGGTCATTGAAAACAACCGTCCACCTCCCGGATGGCCATCATCTGGATCCATAAAATTTGAGGATGTTGTTCTTCGTTACCGCCCTGAGTTACCTCCTGTTCTTCATGGAGTTTCGTTCTTGATTTCTCCAATGGATAAGGTGGGAATTGTTGGGAGGACAGGCGCTGGGAAATCAAGCCTCTTAAATGCCTTATTCAGGATTGTGGAGCTGGAAAAAGGAAGGATTTTAATTGATGAATGCGACATTGGAAGATTTGGACTGATGGACCTACGTAAAGTGCTCGGAATTATACCGCAAGCGCCAGTTCTTTTCTCAGGTACCGTGAGATTCAATCTTGACCCATTTAGTGAACACAACGACGCCGATCTCTGGGAATCTCTTGAGAGGGCACACTTGAAAGATACTATCCGCAGAAATCCTCTTGGTCTTGATGCTGAGGTAACTGAGGCAGGAGAGAATTTCAGTGTTGGACAGAGACAGTTGTTGAGTCTTGCACGTGCATTGTTACGAAGATCTAAGATACTTGTTCTTGATGAAGCAACTGCTGCAGTTGACGTAAGAACTGATGTTCTCATCCAAAAGACCATCCGAGAAGAATTCAAGTCATGCACAATGCTAATCATCGCTCATCGTCTCAATACTATCATCGACTGTGACAAAGTTCTTGTCCTTGATTCTGGAAAAGTTCAGGAATTCAGTTCACCGGAGAATCTTCTTTCAAATGGAGAAAGTTCTTTCTCGAAGATGGTTCAAAGTACAGGAACTGCAAACGCGGAGTACTTACGTAGTATAACACTAGAGAACAAACGTACCAGAGAAGCTAACGGTGATGATTCACAACCTTTAGAAGGTCAAAGGAAATGGCAAGCTTCTTCTCGTTGGGCTGCAGCTGCTCAATTTGCATTGGCTGTGAGCCTCACTTCATCTCACAACGACCTCCAAAGCCTTGAAATCGAAGATGATAACAGTATTTTGAAGAAAACAAAGGACGCCGTCGTCACTCTACGCAGTGTCCTTGAAGGGAAACATGATAAAGAGATTGAAGACTCTCTAAACCAAAGTGACATCTCTAGAGAGCGTTGGTGGCCATCTCTTTACAAAATGGTCGAAGGGCTTGCCGTGATGAGCAGATTGGCGAGGAACAGAATGCAACACCCGGATTACAATTTAGAAGGGAAATCGTTTGACTGGGACAATGTCGAGATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006820 GO:0008150 GO:0008324 GO:0008490 GO:0008509 GO:0009506 GO:0015075 GO:0015103 GO:0015105 GO:0015291 GO:0015318 GO:0015399 GO:0015405 GO:0015446 GO:0015698 GO:0015700 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030054 GO:0031090 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0042946 GO:0042947 GO:0043225 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046685 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071702 GO:0090662 GO:0098588 GO:0098655 GO:0098656 GO:0098660 GO:0098805 GO:0099131 GO:0099132 GO:0099133 GO:1901264 GO:1901505 GO:1901656 GO:1901683 GO:1901684 GO:1902417 GO:1902418

KEGG Pathways

Metabolic & Signaling

ko01523 ko01524 ko02010 ko04071 ko04976 ko04977 ko05206 map01523 map01524 map02010 map04071 map04976 map04977 map05206

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1622

Amino Acids

181.92

Weight (kDa)

5.87

Isoelectric Point (pI)

37.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	306 - 570	9.2e-39	ABC transporter transmembrane region
ABC_tran	PF00005	632 - 767	3.4e-18	ABC transporter
ABC_membrane	PF00664	917 - 1168	2.5e-33	ABC transporter transmembrane region
ABC_tran	PF00005	1254 - 1402	1.6e-24	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30400 AT1G30400 AT2G34660 AT2G34660 AT2G34660
fragaria_vesca	FvH4_2g14980 FvH4_2g14980 FvH4_2g14980
malus_domestica	MD05G1275500.v1.1
prunus_persica	Prupe.4G088500_v2.0.a1 Prupe.4G088500_v2.0.a1 Prupe.4G088500_v2.0.a1 Prupe.4G088500_v2.0.a1 Prupe.4G088500_v2.0.a1
pyrus_communis	pycom05g25670 pycom10g21060
rosa_chinensis	RchiOBHm_Chr5g0015421
rosa_laevigata	RLG00000032198
rosa_multiflora	Rmu_ssc0000168.1_g000001
rosa_roxburghii	Rroxscaffold_1G00061060
rosa_rugosa	Rorug05G0027300
rosa_samantha	Rh5AG120900 Rh5BG119700 Rh5CG130500 Rh5DG118800
rosa_wichuraiana	Rw5G010440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1379, 1730, 3321
AarI	CACCTGC	1 cut(s)	1666
AasI	GACNNNNNNGTC	2 cut(s)	3585, 4655
Acc16I	TGCGCA	1 cut(s)	2316
Acc36I	ACCTGC	2 cut(s)	1666, 2320
Acc65I	GGTACC	2 cut(s)	2715, 3977
AccB1I	GGYRCC	4 cut(s)	37, 1267, 2715, 3977
AccB7I	CCANNNNNTGG	2 cut(s)	864, 3701
AccII	CGCG	2 cut(s)	3584, 4433
AccIII	TCCGGA	1 cut(s)	1545
AciI	CCGC	9 cut(s)	257, 1171, 1457, 1880, 1937, 3739, 3953, 4069, 4433
AclWI	GGATC	7 cut(s)	186, 293, 754, 2041, 2072, 3699, 3712
AcoI	YGGCCR	2 cut(s)	3692, 4745
AcsI	RAATTY	8 cut(s)	628, 694, 2269, 2635, 3713, 4117, 4256, 4352
AcuI	CTGAAG	1 cut(s)	2577
AcvI	CACGTG	1 cut(s)	4157
AcyI	GRCGYC	3 cut(s)	1268, 4020, 4650
AdeI	CACNNNGTG	1 cut(s)	187
AflIII	ACRYGT	2 cut(s)	2277, 3093
AjiI	CACGTC	1 cut(s)	2278
AjnI	CCWGG	8 cut(s)	601, 658, 833, 1825, 1865, 1908, 2176, 2706
AjuI	GAANNNNNNNTTGG	2 cut(s)	3888, 3920
AloI	GAACNNNNNNTCC	4 cut(s)	925, 957, 2640, 2672
Alw21I	GWGCWC	3 cut(s)	880, 978, 3942
Alw26I	GTCTC	7 cut(s)	453, 2621, 2902, 3446, 3571, 4129, 4298
AlwI	GGATC	7 cut(s)	186, 293, 754, 2041, 2072, 3699, 3712
AlwNI	CAGNNNCTG	2 cut(s)	3824, 4424
Ama87I	CYCGRG	1 cut(s)	3228
Aor13HI	TCCGGA	1 cut(s)	1545
AoxI	GGCC	8 cut(s)	956, 1435, 1886, 2256, 2485, 3168, 3692, 4745
ApoI	RAATTY	8 cut(s)	628, 694, 2269, 2635, 3713, 4117, 4256, 4352
ArsI	GACNNNNNNTTYG	2 cut(s)	4311, 4343
AseI	ATTAAT	1 cut(s)	3348
Asp700I	GAANNNNTTC	1 cut(s)	654
Asp718I	GGTACC	2 cut(s)	2715, 3977
AspLEI	GCGC	5 cut(s)	1270, 2317, 2878, 3824, 3961
AspS9I	GGNCC	8 cut(s)	343, 592, 956, 2256, 2486, 2849, 3168, 3925
AsuC2I	CCSGG	2 cut(s)	3687, 4817
AsuHPI	GGTGA	6 cut(s)	754, 2146, 2195, 3296, 4353, 4499
AvaI	CYCGRG	1 cut(s)	3228
AvaII	GGWCC	4 cut(s)	343, 592, 2849, 3925
BaeGI	GKGCMC	2 cut(s)	2308, 4051
BaeI	ACNNNNGTAYC	2 cut(s)	4453, 4486
BalI	TGGCCA	2 cut(s)	3694, 4747
BamHI	GGATCC	1 cut(s)	3704
BanI	GGYRCC	4 cut(s)	37, 1267, 2715, 3977
BanII	GRGCYC	3 cut(s)	978, 2107, 2995
BauI	CACGAG	1 cut(s)	1604
BbrPI	CACGTG	1 cut(s)	4157
BbsI	GAAGAC	1 cut(s)	4710
Bbv12I	GWGCWC	3 cut(s)	880, 978, 3942
BbvCI	CCTCAGC	1 cut(s)	4095
BceAI	ACGGC	4 cut(s)	2243, 3455, 4637, 4763
BcgI	CGANNNNNNTGC	4 cut(s)	3508, 3542, 4757, 4791
BciT130I	CCWGG	8 cut(s)	603, 660, 835, 1827, 1867, 1910, 2178, 2708
BciVI	GTATCC	4 cut(s)	1459, 1765, 1850, 3320
BclI	TGATCA	1 cut(s)	2910
BcnI	CCSGG	2 cut(s)	3687, 4817
BcoDI	GTCTC	7 cut(s)	453, 2621, 2902, 3446, 3571, 4129, 4298
BfaI	CTAG	8 cut(s)	848, 1931, 2253, 2360, 3306, 3590, 4457, 4731
BfmI	CTRYAG	4 cut(s)	1229, 2609, 4206, 4545
BfoI	RGCGCY	3 cut(s)	1271, 3825, 3962
BfuAI	ACCTGC	2 cut(s)	1666, 2320
BfuI	GTATCC	4 cut(s)	1459, 1765, 1850, 3320
BglI	GCCNNNNNGGC	1 cut(s)	2484
BglII	AGATCT	3 cut(s)	1648, 2006, 4172
BmcAI	AGTACT	3 cut(s)	500, 2379, 4439
Bme18I	GGWCC	4 cut(s)	343, 592, 2849, 3925
BmeT110I	CYCGRG	1 cut(s)	3228
BmgBI	CACGTC	1 cut(s)	2278
BmgT120I	GGNCC	8 cut(s)	343, 592, 956, 2256, 2486, 2849, 3168, 3925
BmrI	ACTGGG	1 cut(s)	4858
BmuI	ACTGGG	1 cut(s)	4858
BpiI	GAAGAC	1 cut(s)	4710
BplI	GAGNNNNNCTC	2 cut(s)	2172, 2204
BpmI	CTGGAG	5 cut(s)	681, 2138, 2199, 2706, 2729
Bpu10I	CCTNAGC	2 cut(s)	1112, 4095
BpuEI	CTTGAG	5 cut(s)	223, 368, 2186, 2763, 4061
BpuMI	CCSGG	2 cut(s)	3687, 4817
Bsa29I	ATCGAT	1 cut(s)	3040
BsaAI	YACGTR	5 cut(s)	1523, 2084, 3932, 4157, 4445
BsaBI	GATNNNNATC	2 cut(s)	3066, 3694
BsaHI	GRCGYC	3 cut(s)	1268, 4020, 4650
BsaI	GGTCTC	2 cut(s)	2902, 3446
BsaJI	CCNNGG	7 cut(s)	601, 633, 834, 863, 892, 1506, 1866
BsaWI	WCCGGW	2 cut(s)	1545, 4363
BsaXI	ACNNNNNCTCC	8 cut(s)	251, 281, 450, 480, 1281, 1311, 1766, 1796
Bse118I	RCCGGY	2 cut(s)	34, 1107
Bse1I	ACTGG	8 cut(s)	614, 1296, 2582, 2689, 2937, 3266, 3962, 4853
Bse3DI	GCAATG	2 cut(s)	1467, 1634
Bse8I	GATNNNNATC	2 cut(s)	3066, 3694
BseAI	TCCGGA	1 cut(s)	1545
BseBI	CCWGG	8 cut(s)	603, 660, 835, 1827, 1867, 1910, 2178, 2708
BseCI	ATCGAT	1 cut(s)	3040
BseDI	CCNNGG	7 cut(s)	601, 633, 834, 863, 892, 1506, 1866
BseGI	GGATG	8 cut(s)	310, 778, 2623, 3342, 3695, 3727, 4233, 4245
BseJI	GATNNNNATC	2 cut(s)	3066, 3694
BseMI	GCAATG	2 cut(s)	1467, 1634
BseNI	ACTGG	8 cut(s)	614, 1296, 2582, 2689, 2937, 3266, 3962, 4853
BseRI	GAGGAG	5 cut(s)	678, 832, 1289, 1659, 1787
BseSI	GKGCMC	2 cut(s)	2308, 4051
BseYI	CCCAGC	3 cut(s)	311, 3412, 3824
BsgI	GTGCAG	1 cut(s)	1104
Bsh1236I	CGCG	2 cut(s)	3584, 4433
BshFI	GGCC	8 cut(s)	958, 1437, 1888, 2258, 2487, 3170, 3694, 4747
BshNI	GGYRCC	4 cut(s)	37, 1267, 2715, 3977
BshVI	ATCGAT	1 cut(s)	3040
BsiHKAI	GWGCWC	3 cut(s)	880, 978, 3942
BsiHKCI	CYCGRG	1 cut(s)	3228
BsiSI	CCGG	6 cut(s)	35, 1108, 1546, 3687, 4364, 4817
BslFI	GGGAC	4 cut(s)	634, 2241, 3145, 4865
BsmAI	GTCTC	7 cut(s)	453, 2621, 2902, 3446, 3571, 4129, 4298
BsmBI	CGTCTC	1 cut(s)	4298
BsmFI	GGGAC	4 cut(s)	634, 2241, 3145, 4865
BsmI	GAATGC	4 cut(s)	1593, 2728, 3902, 4812
BsnI	GGCC	8 cut(s)	958, 1437, 1888, 2258, 2487, 3170, 3694, 4747
Bso31I	GGTCTC	2 cut(s)	2902, 3446
BsoBI	CYCGRG	1 cut(s)	3228
Bsp1286I	GDGCHC	7 cut(s)	880, 978, 2107, 2308, 2995, 3942, 4051
Bsp13I	TCCGGA	1 cut(s)	1545
Bsp68I	TCGCGA	1 cut(s)	3584
BspACI	CCGC	9 cut(s)	257, 1171, 1457, 1880, 1937, 3739, 3953, 4069, 4433
BspANI	GGCC	8 cut(s)	958, 1437, 1888, 2258, 2487, 3170, 3694, 4747
BspDI	ATCGAT	1 cut(s)	3040
BspEI	TCCGGA	1 cut(s)	1545
BspFNI	CGCG	2 cut(s)	3584, 4433
BspHI	TCATGA	2 cut(s)	279, 378
BspMAI	CTGCAG	4 cut(s)	1233, 2613, 4210, 4549
BspMI	ACCTGC	2 cut(s)	1666, 2320
BspPI	GGATC	7 cut(s)	186, 293, 754, 2041, 2072, 3699, 3712
BspQI	GCTCTTC	1 cut(s)	645
BspT107I	GGYRCC	4 cut(s)	37, 1267, 2715, 3977
BspTNI	GGTCTC	2 cut(s)	2902, 3446
BsrDI	GCAATG	2 cut(s)	1467, 1634
BsrFI	RCCGGY	2 cut(s)	34, 1107
BsrI	ACTGG	8 cut(s)	614, 1296, 2582, 2689, 2937, 3266, 3962, 4853
BssAI	RCCGGY	2 cut(s)	34, 1107
BssECI	CCNNGG	7 cut(s)	601, 633, 834, 863, 892, 1506, 1866
BssNI	GRCGYC	3 cut(s)	1268, 4020, 4650
BssSI	CACGAG	1 cut(s)	1604
BssT1I	CCWWGG	2 cut(s)	892, 1506
Bst2BI	CACGAG	1 cut(s)	1604
Bst2UI	CCWGG	8 cut(s)	603, 660, 835, 1827, 1867, 1910, 2178, 2708
Bst6I	CTCTTC	3 cut(s)	645, 966, 1785
BstACI	GRCGYC	3 cut(s)	1268, 4020, 4650
BstAPI	GCANNNNNTGC	2 cut(s)	1127, 4205
BstBAI	YACGTR	5 cut(s)	1523, 2084, 3932, 4157, 4445
BstC8I	GCNNGC	6 cut(s)	32, 690, 1836, 3957, 4527, 4776
BstDSI	CCRYGG	1 cut(s)	863
BstEII	GGTNACC	2 cut(s)	1103, 2183
BstENI	CCTNNNNNAGG	2 cut(s)	3853, 4506
BstF5I	GGATG	8 cut(s)	310, 778, 2623, 3342, 3695, 3727, 4233, 4245
BstFNI	CGCG	2 cut(s)	3584, 4433
BstH2I	RGCGCY	3 cut(s)	1271, 3825, 3962
BstHHI	GCGC	5 cut(s)	1270, 2317, 2878, 3824, 3961
BstMAI	GTCTC	7 cut(s)	453, 2621, 2902, 3446, 3571, 4129, 4298
BstNI	CCWGG	8 cut(s)	603, 660, 835, 1827, 1867, 1910, 2178, 2708
BstNSI	RCATGY	3 cut(s)	692, 2321, 3097
BstPI	GGTNACC	2 cut(s)	1103, 2183
BstSFI	CTRYAG	4 cut(s)	1229, 2609, 4206, 4545
BstSLI	GKGCMC	2 cut(s)	2308, 4051
BstSNI	TACGTA	2 cut(s)	3932, 4445
BstUI	CGCG	2 cut(s)	3584, 4433
BstV2I	GAAGAC	1 cut(s)	4710
BstX2I	RGATCY	6 cut(s)	285, 1648, 2006, 2064, 3704, 4172
BstYI	RGATCY	6 cut(s)	285, 1648, 2006, 2064, 3704, 4172
Bsu15I	ATCGAT	1 cut(s)	3040
BsuI	GTATCC	4 cut(s)	1459, 1765, 1850, 3320
BsuRI	GGCC	8 cut(s)	958, 1437, 1888, 2258, 2487, 3170, 3694, 4747
BsuTUI	ATCGAT	1 cut(s)	3040
BtgI	CCRYGG	1 cut(s)	863
BtgZI	GCGATG	1 cut(s)	4267
BtrI	CACGTC	1 cut(s)	2278
BtsCI	GGATG	8 cut(s)	310, 778, 2623, 3342, 3695, 3727, 4233, 4245
BtsI	GCAGTG	1 cut(s)	4675
BtsIMutI	CAGTG	7 cut(s)	878, 1121, 1289, 1476, 2227, 4129, 4675
BtuMI	TCGCGA	1 cut(s)	3584
BveI	ACCTGC	2 cut(s)	1666, 2320
Cac8I	GCNNGC	6 cut(s)	32, 690, 1836, 3957, 4527, 4776
CaiI	CAGNNNCTG	2 cut(s)	3824, 4424
CciI	TCATGA	2 cut(s)	279, 378
CfoI	GCGC	5 cut(s)	1270, 2317, 2878, 3824, 3961
Cfr10I	RCCGGY	2 cut(s)	34, 1107
Cfr13I	GGNCC	8 cut(s)	343, 592, 956, 2256, 2486, 2849, 3168, 3925
ClaI	ATCGAT	1 cut(s)	3040
CseI	GACGC	3 cut(s)	3450, 4028, 4658
DinI	GGCGCC	1 cut(s)	1269
DraI	TTTAAA	2 cut(s)	514, 3606
DraIII	CACNNNGTG	1 cut(s)	187
DrdI	GACNNNNNNGTC	2 cut(s)	3585, 4655
DseDI	GACNNNNNNGTC	2 cut(s)	3585, 4655
EaeI	YGGCCR	2 cut(s)	3692, 4745
Eam1104I	CTCTTC	3 cut(s)	645, 966, 1785
EarI	CTCTTC	3 cut(s)	645, 966, 1785
EciI	GGCGGA	2 cut(s)	1186, 1895
Ecl136II	GAGCTC	1 cut(s)	976
Eco105I	TACGTA	2 cut(s)	3932, 4445
Eco130I	CCWWGG	2 cut(s)	892, 1506
Eco24I	GRGCYC	3 cut(s)	978, 2107, 2995
Eco31I	GGTCTC	2 cut(s)	2902, 3446
Eco32I	GATATC	1 cut(s)	1977
Eco47I	GGWCC	4 cut(s)	343, 592, 2849, 3925
Eco53kI	GAGCTC	1 cut(s)	976
Eco57I	CTGAAG	1 cut(s)	2577
Eco72I	CACGTG	1 cut(s)	4157
Eco88I	CYCGRG	1 cut(s)	3228
Eco91I	GGTNACC	2 cut(s)	1103, 2183
EcoICRI	GAGCTC	1 cut(s)	976
EcoNI	CCTNNNNNAGG	2 cut(s)	3853, 4506
EcoO109I	RGGNCCY	1 cut(s)	956
EcoO65I	GGTNACC	2 cut(s)	1103, 2183
EcoRI	GAATTC	3 cut(s)	2635, 4256, 4352
EcoRII	CCWGG	8 cut(s)	601, 658, 833, 1825, 1865, 1908, 2176, 2706
EcoRV	GATATC	1 cut(s)	1977
EcoT14I	CCWWGG	2 cut(s)	892, 1506
EcoT22I	ATGCAT	3 cut(s)	2348, 2964, 3514
EcoT38I	GRGCYC	3 cut(s)	978, 2107, 2995
EgeI	GGCGCC	1 cut(s)	1269
EheI	GGCGCC	1 cut(s)	1269
ErhI	CCWWGG	2 cut(s)	892, 1506
Esp3I	CGTCTC	1 cut(s)	4298
FalI	AAGNNNNNCTT	4 cut(s)	185, 217, 2788, 2820
FaqI	GGGAC	4 cut(s)	634, 2241, 3145, 4865
FauNDI	CATATG	3 cut(s)	2464, 3328, 3508
FbaI	TGATCA	1 cut(s)	2910
FokI	GGATG	8 cut(s)	317, 785, 2630, 3349, 3702, 3734, 4220, 4232
FriOI	GRGCYC	3 cut(s)	978, 2107, 2995
FspAI	RTGCGCAY	1 cut(s)	2316
FspBI	CTAG	8 cut(s)	848, 1931, 2253, 2360, 3306, 3590, 4457, 4731
FspI	TGCGCA	1 cut(s)	2316
GlaI	GCGC	5 cut(s)	1269, 2316, 2877, 3823, 3960
GsaI	CCCAGC	3 cut(s)	315, 3416, 3828
GsuI	CTGGAG	5 cut(s)	681, 2138, 2199, 2706, 2729
HaeII	RGCGCY	3 cut(s)	1271, 3825, 3962
HaeIII	GGCC	8 cut(s)	958, 1437, 1888, 2258, 2487, 3170, 3694, 4747
HapII	CCGG	6 cut(s)	35, 1108, 1546, 3687, 4364, 4817
HgaI	GACGC	3 cut(s)	3450, 4028, 4658
HhaI	GCGC	5 cut(s)	1270, 2317, 2878, 3824, 3961
Hin1I	GRCGYC	3 cut(s)	1268, 4020, 4650
Hin6I	GCGC	5 cut(s)	1268, 2315, 2876, 3822, 3959
HinP1I	GCGC	5 cut(s)	1268, 2315, 2876, 3822, 3959
HincII	GTYRAC	5 cut(s)	1398, 1785, 2215, 3456, 4213
HindII	GTYRAC	5 cut(s)	1398, 1785, 2215, 3456, 4213
HindIII	AAGCTT	1 cut(s)	4527
HpaI	GTTAAC	2 cut(s)	1398, 2215
HpaII	CCGG	6 cut(s)	35, 1108, 1546, 3687, 4364, 4817
HphI	GGTGA	6 cut(s)	754, 2146, 2195, 3296, 4353, 4499
Hpy99I	CGWCG	2 cut(s)	4022, 4658
Hsp92I	GRCGYC	3 cut(s)	1268, 4020, 4650
HspAI	GCGC	5 cut(s)	1268, 2315, 2876, 3822, 3959
KasI	GGCGCC	1 cut(s)	1267
Kpn2I	TCCGGA	1 cut(s)	1545
KpnI	GGTACC	2 cut(s)	2719, 3981
Ksp22I	TGATCA	1 cut(s)	2910
KspAI	GTTAAC	2 cut(s)	1398, 2215
LguI	GCTCTTC	1 cut(s)	645
LmnI	GCTCC	9 cut(s)	259, 308, 819, 2110, 2400, 2998, 3200, 3658, 3865
MaeI	CTAG	8 cut(s)	848, 1931, 2253, 2360, 3306, 3590, 4457, 4731
MfeI	CAATTG	1 cut(s)	1310
MflI	RGATCY	6 cut(s)	285, 1648, 2006, 2064, 3704, 4172
MhlI	GDGCHC	7 cut(s)	880, 978, 2107, 2308, 2995, 3942, 4051
MlsI	TGGCCA	2 cut(s)	3694, 4747
MluNI	TGGCCA	2 cut(s)	3694, 4747
Mly113I	GGCGCC	1 cut(s)	1268
MlyI	GAGTC	9 cut(s)	98, 733, 1232, 2770, 2836, 3573, 3595, 4156, 4700
MmeI	TCCRAC	4 cut(s)	1024, 2678, 3604, 4108
Mox20I	TGGCCA	2 cut(s)	3694, 4747
Mph1103I	ATGCAT	3 cut(s)	2348, 2964, 3514
MroI	TCCGGA	1 cut(s)	1545
MroXI	GAANNNNTTC	1 cut(s)	654
MscI	TGGCCA	2 cut(s)	3694, 4747
MslI	CAYNNNNRTG	1 cut(s)	3098
Msp20I	TGGCCA	2 cut(s)	3694, 4747
MspA1I	CMGCKG	2 cut(s)	3569, 4550
MspI	CCGG	6 cut(s)	35, 1108, 1546, 3687, 4364, 4817
MssI	GTTTAAAC	1 cut(s)	3606
MunI	CAATTG	1 cut(s)	1310
Mva1269I	GAATGC	4 cut(s)	1593, 2728, 3902, 4812
MvaI	CCWGG	8 cut(s)	603, 660, 835, 1827, 1867, 1910, 2178, 2708
MvnI	CGCG	2 cut(s)	3584, 4433
NarI	GGCGCC	1 cut(s)	1268
NciI	CCSGG	2 cut(s)	3687, 4817
NdeI	CATATG	3 cut(s)	2464, 3328, 3508
NmuCI	GTSAC	9 cut(s)	1606, 1674, 2084, 2143, 2183, 2902, 4313, 4657, 4721
NruI	TCGCGA	1 cut(s)	3584
NsbI	TGCGCA	1 cut(s)	2316
NsiI	ATGCAT	3 cut(s)	2348, 2964, 3514
NspI	RCATGY	3 cut(s)	692, 2321, 3097
PaeI	GCATGC	1 cut(s)	692
PagI	TCATGA	2 cut(s)	279, 378
PaqCI	CACCTGC	1 cut(s)	1666
PciI	ACATGT	1 cut(s)	3093
PciSI	GCTCTTC	1 cut(s)	645
PctI	GAATGC	4 cut(s)	1593, 2728, 3902, 4812
PdmI	GAANNNNTTC	1 cut(s)	654
PflFI	GACNNNGTC	3 cut(s)	3460, 4652, 4856
PflMI	CCANNNNNTGG	2 cut(s)	864, 3701
PfoI	TCCNGGA	3 cut(s)	658, 2706, 3685
PleI	GAGTC	9 cut(s)	98, 733, 1231, 2770, 2836, 3573, 3594, 4155, 4700
PluTI	GGCGCC	1 cut(s)	1271
PmaCI	CACGTG	1 cut(s)	4157
PmeI	GTTTAAAC	1 cut(s)	3606
PmlI	CACGTG	1 cut(s)	4157
PpsI	GAGTC	9 cut(s)	98, 733, 1231, 2770, 2836, 3573, 3594, 4155, 4700
Ppu21I	YACGTR	5 cut(s)	1523, 2084, 3932, 4157, 4445
PscI	ACATGT	1 cut(s)	3093
PshBI	ATTAAT	1 cut(s)	3348
PsiI	TTATAA	3 cut(s)	1379, 1730, 3321
Psp124BI	GAGCTC	1 cut(s)	978
Psp6I	CCWGG	8 cut(s)	601, 658, 833, 1825, 1865, 1908, 2176, 2706
PspCI	CACGTG	1 cut(s)	4157
PspEI	GGTNACC	2 cut(s)	1103, 2183
PspFI	CCCAGC	3 cut(s)	311, 3412, 3824
PspGI	CCWGG	8 cut(s)	601, 658, 833, 1825, 1865, 1908, 2176, 2706
PspPI	GGNCC	8 cut(s)	343, 592, 956, 2256, 2486, 2849, 3168, 3925
PsrI	GAACNNNNNNTAC	2 cut(s)	1278, 1310
PstI	CTGCAG	4 cut(s)	1233, 2613, 4210, 4549
PstNI	CAGNNNCTG	2 cut(s)	3824, 4424
PsuI	RGATCY	6 cut(s)	285, 1648, 2006, 2064, 3704, 4172
PsyI	GACNNNGTC	3 cut(s)	3460, 4652, 4856
PvuII	CAGCTG	2 cut(s)	3569, 4550
RruI	TCGCGA	1 cut(s)	3584
RseI	CAYNNNNRTG	1 cut(s)	3098
SacI	GAGCTC	1 cut(s)	978
SapI	GCTCTTC	1 cut(s)	645
Sau96I	GGNCC	8 cut(s)	343, 592, 956, 2256, 2486, 2849, 3168, 3925
ScaI	AGTACT	3 cut(s)	500, 2379, 4439
SchI	GAGTC	9 cut(s)	98, 733, 1232, 2770, 2836, 3573, 3595, 4156, 4700
SduI	GDGCHC	7 cut(s)	880, 978, 2107, 2308, 2995, 3942, 4051
SfcI	CTRYAG	4 cut(s)	1229, 2609, 4206, 4545
SfoI	GGCGCC	1 cut(s)	1269
SinI	GGWCC	4 cut(s)	343, 592, 2849, 3925
SmiMI	CAYNNNNRTG	1 cut(s)	3098
SmlI	CTYRAG	5 cut(s)	202, 347, 2165, 2778, 4040
SmoI	CTYRAG	5 cut(s)	202, 347, 2165, 2778, 4040
SnaBI	TACGTA	2 cut(s)	3932, 4445
SphI	GCATGC	1 cut(s)	692
SsiI	CCGC	9 cut(s)	257, 1171, 1457, 1880, 1937, 3739, 3953, 4069, 4433
SspDI	GGCGCC	1 cut(s)	1267
SspI	AATATT	1 cut(s)	2866
SspMI	CTAG	8 cut(s)	848, 1931, 2253, 2360, 3306, 3590, 4457, 4731
SstI	GAGCTC	1 cut(s)	978
StyI	CCWWGG	2 cut(s)	892, 1506
TatI	WGTACW	5 cut(s)	498, 2377, 3571, 4415, 4437
TauI	GCSGC	1 cut(s)	1460
TscAI	CASTG	7 cut(s)	885, 1128, 1296, 1476, 2227, 4129, 4675
TseFI	GTSAC	9 cut(s)	1606, 1674, 2084, 2143, 2183, 2902, 4313, 4657, 4721
Tsp45I	GTSAC	9 cut(s)	1606, 1674, 2084, 2143, 2183, 2902, 4313, 4657, 4721
TspGWI	ACGGA	6 cut(s)	407, 2207, 3043, 3302, 3410, 3498
TspRI	CASTG	7 cut(s)	885, 1128, 1296, 1476, 2227, 4129, 4675
Tth111I	GACNNNGTC	3 cut(s)	3460, 4652, 4856
Van91I	CCANNNNNTGG	2 cut(s)	864, 3701
VpaK11BI	GGWCC	4 cut(s)	343, 592, 2849, 3925
VspI	ATTAAT	1 cut(s)	3348
XagI	CCTNNNNNAGG	2 cut(s)	3853, 4506
XapI	RAATTY	8 cut(s)	628, 694, 2269, 2635, 3713, 4117, 4256, 4352
XbaI	TCTAGA	1 cut(s)	4730
XceI	RCATGY	3 cut(s)	692, 2321, 3097
XcmI	CCANNNNNNNNNTGG	3 cut(s)	2058, 2726, 3687
XmnI	GAANNNNTTC	1 cut(s)	654
XspI	CTAG	8 cut(s)	848, 1931, 2253, 2360, 3306, 3590, 4457, 4731
ZrmI	AGTACT	3 cut(s)	500, 2379, 4439
Zsp2I	ATGCAT	3 cut(s)	2348, 2964, 3514

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.