Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006461

Physical Location & Seq

Genomic Coordinates Reverse (-)

40978430 .. 40997607

Gene Structure 19178 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1275500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4557 bp

ATGGGTTTTCAGCTTTTGGATTGGTATTGCCGCCCGGTGCCCGGTGGGGTCTGGACAAGAGCAGTGGAGAATGCTTTTGGTGCATACACGCCGTGCACGGTTGATTCTTTAGTTGTCTGCATTTCTCATTTGGTTGTTCTGGGCCTGTGCATTTATCGAATATGGCGGATCAAGAAGGATTTCAAAGTTCAGAGGTTCTGTTTGCAGTCCAAAGTTTACAACTACATGTTGGGGTTATTGGCTGCTTATTGCACTGCCGAGCCTGTGTTCCGGTTGATCATGGGAATTTCAGTGCTGAATTTAGATGGGCAGACTGGCCTTGCACCATTTGAGGTATTTTCTCTGATATTAGAGGCTCTTACTTGGTTCTCTACGCTGGTCATGATTGGTGTGGAATCTAAGATCTATATCCGTGAGGTCCGATGGTTTGTAAGGTTTGCGGTAATTTATACTTTAGTGGGGGATTCCGTGATGCTGAATCTTGTTCTTTCACTGAGGGAGTTATACGACAGATCTGTACTATATTTGTACATCAGTGAAGTTGCTGCTCAGGGTTTGTTTGGTATACTTTTGCTTGTGTACGTTCCATTTGTGGTCGATTATCCTGGTTATACTCCAATCCGTACTGAATCAATTGATGATGATGCATATGAAGAACTTCCTGGAGGAGAGCAAATATGTCCCGAGAGGCATGTGAACATATTGTCAAGAATGCTGTTTTCATGGGTGAATCCCCTTATGAAGCTAGGCTATCAAAGACCCATTACAGAGAAAGATGTCTGGAAATTGGATACTTGGGATCGTACTGAAACACTGAACAATAAGTTCCAAACATGTTGGGCTGAGGAATGTCGAAAGCCTAAACCATGGCTTTTAAGAGCATTGAACTCTAGTCTTGGCGGAAGTTCTATGTTTTGGTTTGGAGGCTTTTGGAAGATCGGGAATGATCTTTCCCAATTTGTGGGGCCGCTTATTTTGAATCATCTCTTACAGTCTATGCAACGAGGGGATCCAGCATGGATTGGTCACATCTATGCCTTCTCAATATTTGCTGGAGTGGTTGGTGGGGTGTTATCTGAAGCTCAGTATTTTCAAAATGTCATGCGCGTTGGCTTCCGGCTACGGTCAACCCTGGTTGCAGCTGTATTTCGGAAATCCTTTAGGCTGACTCATGAAGCAAGGAAGAAGTTTGCCTCTGGGAAAATAACCAACTTGATGACAACTGATGCTGAAGCACTTCAGGCACGCACTCTGAACAGTTTTTGCATGTTATCCCAATCTCTTCATATTTTGTGGTCAGCTCCATTTCGTATCGTTTTCTCCATGGTTCTTCTTTATCAGCAATTGGGGGTTGCTTCACTACTTGGTGCCCTCATGCTTGTTCTTTTGTTCCCCCTACAGACATTTGTGATAAGCAAAATGCAGAAATTGTCCAAGGAGGGGTTGCAGCGTACAGACAAGAGAATTGGTCTGATGAATGAAATTTTGGCTGCAATGGACACTGTGAAATGTTATGCATGGGAGAGTAGTTTCCAATCCAAAGTGCATAGTGTTCGAACTGATGAGTTGTTCTGGTTTCGAAAAGCATCACTGCTGGGAGCGTGCAACGGATTTATACTAAATAGCAGCCCAGTGGTAGTCACCGTGATTTCATTTGGATTGTTCACTTTTCTTGGGGGGGATCTTACACCTGCAAGGGCATTTACATCACTTACTCTGTTTGCTGTGCTTCGGTTTCCCCTATTCATACTTCCTAATATAATAACCCGGGTGGCTAATGCTAATGTATCCTTAAAGCGCTTAGAGGAGCTACTTTTAGCTGAAGAGAGAGTTCTTCTGCCAAACCCTCCTCTTGATCCAGGACTGCCGGCCATCTCAATTAGGAATGGATACTTTTCTTGGGACTCAAAGGCAGAGAAGCCCACGCTGACAGATGTTAATTTGGATATCCCTGTTGGTAACTTGGTAGCAATCGTTGGCGGTACTGGTGAAGGAAAGACGTCACTTGTATCTGCAATGATTGGGGAGCTTCCTCCTGTAGCAGAATCAAGTGTTGTAATGAGAGGAATGGTTGCTTATGTTCCACAAGTTTCATGGATTTTTAATGCAACTGTACGTGACAACATTTTATTTGGATCTTACTTTGACTCCAGAAGATATGAGAAGGCAATAGATGTGACTGCATTGAGGCAGGACCTTGACTTGTTGCCTGGTGGTGATCTGACTGAGATTGGTGAAAGAGGAGTGAATATTAGTGGTGGGCAGAAGCAAAGAGTCTCCATGGCTAGAGCTGTCTACTCCAGCTCTGATGTATACATTTTTGATGATCCCTTGAGTGCTCTGGATGCTCAAGTTGCTCGACAGGTTTTTGATAAATGTATCAGGGGAGAACTGAGAGGGAAAACCAGGGTTCTTGTTACAAATCAGCTGCATTTCCTTTCACAGGTTGATAGAATCATCCTGGTCCATGAAGGTATGGTGAAGGAGGAGGGAACATTTGAGGAGCTTTCCAATAATGGCACGCTGTTCAAAAGGCTAATGGAAAAAGCGGGAAAGATGGAAGAATGTGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAAAGAAGATAGTAGAACCTTTGATCAAAACAGTGAAATGATTGATCATAGTGCCTCTTCAAAACCTGTTGCCAATGGGGTGGTAAATGGTATGCCAAAAAATACAAGCCAAGCCAATAAACCAAAAGAAGGAAAATCTGTTCTTATCAAACAAGAAGAAAAGGAAACAGGCGTAGTAAGCTTGAAAGTTTTGGCGAGGTATAAGGATGCGTTAGGAGGCTTGTGGGCGGTTATGATACTCTTTACATGCTATGTTTCATCCGAGGTTCTACGAGTTTCAAGTAGCACATGGTTGAGCCATTGGACTGATCAAGGCACGACAGCGAATTATAACCCCGGTTTCTACAATATGATATATGCACTAATATCATTTGGTCAGGTTTTGGTGACATTAGCAAACTCTTATTGGTTGATTATTTCTAGTCTTTATGCTGCTAGGAGGTTGCATGAGGCCATGCTTAGCTCTATCTTAAAAGCTCCAATGGTCTTCTTCCAAACCAATCCCCTTGGACGGATCATAAATAGATTTGCAAAGGATCTTGGAGACATTGATCGTAATGTTGCTCCATTTGTGAATATGTTTCTTGGGCAAGCCTCTCAGCTCCTTTCTACCTTCATCTTGATAGGGATCGTGAGCACTATGTCCTTGTGGGCCATTATGCCCCTTCTCGTTTTGTTTTATGCAGCCTATCTCTACTATCAGAGCACGGCTCGTGAGTTAAAGCGCTTGGACTCCATCAGCAGATCCCCTGTTTATGCACAATTTGGTGAAGCACTGAATGGTTTATCAACTATCCGCGCATACAAGGCTTATGATCGGATGGCAGACATTAATGGAAGATCTGTGGATACCAATATCAGATTTGCACTGTTGACCATAAGTGGAAACCGCTGGCTTGCAATCCGTTTGGAAACATTGGGAGGCCTCATGATATGGTTTACGGCTACCTTTGCTGTTATGAAGAATGGAAGGGCAGAGAACCAGCAAGAGTTTGCATCCACTATGGGTCTACTTCTCAGTTATGCGCTTAATATTACAGATTTATTGACTGCTGTACTGAGACTTGCAAGTCAGGCTGAGAATAGCTTGAATGCTGTTGAGCGGGTCGGTACATACATAGAGTTGCCTTCAGAAGCTCCAGCTGTAATTGAGAGCAACCGCCCCCCTCCAGCGTGGCCTTCATTTGGATATATCAAGTTTGAGAATGTTGTTCTACGTTATAGACCGGAACTTCCCCCAGTTTTGCTTGAGTTGTCTTTCACAATTTCTCCTACTGACAAGGTCGGAATTGTTGGAAGGACTGGCGCTGGAAAGTCCAGCATGCTAAATGCTTTATTTCGGATTGTTGAATTAGAAAGAGGAAGAATTTTGATTGATGATTGTGATATCGCTAAGTTTGGACTGTCAGATTTACGTAAAGTTCTTGGTATCATACCTCAGTCACCGGTTCTTTTTTCAGGAACAGTGAGGTTTAATCTCGATCCCTTTCAAGAACATAATGATGCTGACCTCTGGGAAGCTTTAGAGAGGGCACATTTGAAGGATGCCATCAGGAGGAATTCTTTGGGATTAGATGCTGAGGTCTCGGAGGCAGGAGATAATTTCAGTGTTGGACAGCGACAACTATTAAGTCTTTCTCGAGCAGTGTTGCGAAGATCAAAAATACTCGTTCTTGATGAAGCAACTGCAGCTGTTGATGTCAGAACTGATGCTCTTATTCAAAAAACTATTCGTCAAGAATTTCAATCATGTACAATGCTCATCATTGCTCATCGTCTGAATACCATTATTGACTGCGACCGAATCCTTCTGCTTGATGCTGGCCGGGTTCGAGAATTCGATACACCAGAGCGCCTGCTGTCTAATGAAGGAAGTGCTTTCTCCAAAATGGTCCAAAGTACAGGCTCAGCGAATGCTCAGTATTTACGAAGCTTGGTACTTGGAGGGGAAGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0008150 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031090 GO:0042623 GO:0042626 GO:0042946 GO:0042947 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0098588 GO:0098805 GO:1901264 GO:1901505 GO:1901656 GO:1902417 GO:1902418

KEGG Pathways

Metabolic & Signaling

ko01523 ko01524 ko02010 ko04071 ko04976 ko04977 ko05206 map01523 map01524 map02010 map04071 map04976 map04977 map05206

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1519

Amino Acids

169.9

Weight (kDa)

6.79

Isoelectric Point (pI)

43.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	306 - 581	1.1e-30	ABC transporter transmembrane region
ABC_tran	PF00005	643 - 778	2e-17	ABC transporter
ABC_membrane	PF00664	945 - 1185	8.6e-33	ABC transporter transmembrane region
ABC_tran	PF00005	1284 - 1430	3e-24	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30400 AT1G30400 AT2G34660 AT2G34660 AT2G34660
fragaria_vesca	FvH4_2g14980 FvH4_2g14980 FvH4_2g14980
malus_domestica	MD05G1275500.v1.1
prunus_persica	Prupe.4G088500_v2.0.a1 Prupe.4G088500_v2.0.a1 Prupe.4G088500_v2.0.a1 Prupe.4G088500_v2.0.a1 Prupe.4G088500_v2.0.a1
pyrus_communis	pycom05g25670 pycom10g21060
rosa_chinensis	RchiOBHm_Chr5g0015421
rosa_laevigata	RLG00000032198
rosa_multiflora	Rmu_ssc0000168.1_g000001
rosa_roxburghii	Rroxscaffold_1G00061060
rosa_rugosa	Rorug05G0027300
rosa_samantha	Rh5AG120900 Rh5BG119700 Rh5CG130500 Rh5DG118800
rosa_wichuraiana	Rw5G010440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2931
AarI	CACCTGC	1 cut(s)	1699
AasI	GACNNNNNNGTC	1 cut(s)	3669
AatII	GACGTC	1 cut(s)	2003
Acc36I	ACCTGC	1 cut(s)	1699
AccB1I	GGYRCC	2 cut(s)	37, 1367
AccB7I	CCANNNNNTGG	1 cut(s)	961
AccBSI	CCGCTC	1 cut(s)	3703
AccI	GTMKAC	4 cut(s)	565, 2295, 2313, 3610
AccII	CGCG	2 cut(s)	1107, 3399
AcoI	YGGCCR	2 cut(s)	1869, 4423
AcsI	RAATTY	7 cut(s)	285, 298, 1482, 3966, 4159, 4340, 4436
AcuI	CTGAAG	5 cut(s)	1098, 1223, 1251, 1842, 3714
AcyI	GRCGYC	1 cut(s)	2000
AdeI	CACNNNGTG	1 cut(s)	93
AfeI	AGCGCT	2 cut(s)	1799, 3326
AfiI	CCNNNNNNNGG	8 cut(s)	41, 961, 1440, 2443, 2729, 3111, 3785, 4155
AflIII	ACRYGT	2 cut(s)	225, 833
AgeI	ACCGGT	1 cut(s)	4045
AjnI	CCWGG	7 cut(s)	604, 661, 1131, 1858, 2209, 2405, 2460
AjuI	GAANNNNNNNTTGG	2 cut(s)	1469, 1501
AloI	GAACNNNNNNTCC	2 cut(s)	2724, 2756
Alw21I	GWGCWC	4 cut(s)	98, 2341, 3239, 3308
Alw26I	GTCTC	4 cut(s)	2281, 3138, 3655, 4189
Alw44I	GTGCAC	1 cut(s)	94
Ama87I	CYCGRG	3 cut(s)	683, 1767, 4239
Aor51HI	AGCGCT	2 cut(s)	1799, 3326
AoxI	GGCC	9 cut(s)	142, 316, 965, 1869, 3051, 3252, 3523, 3776, 4423
ApaLI	GTGCAC	1 cut(s)	94
ApoI	RAATTY	7 cut(s)	285, 298, 1482, 3966, 4159, 4340, 4436
AseI	ATTAAT	1 cut(s)	3432
AsiGI	ACCGGT	1 cut(s)	4045
Asp700I	GAANNNNTTC	3 cut(s)	179, 657, 1236
AspLEI	GCGC	7 cut(s)	1107, 1800, 3327, 3401, 3628, 3908, 4455
AspS9I	GGNCC	7 cut(s)	142, 418, 965, 2194, 2464, 3252, 4492
AsuC2I	CCSGG	6 cut(s)	35, 42, 1768, 1769, 2937, 4427
AsuHPI	GGTGA	9 cut(s)	739, 1633, 2000, 2228, 2246, 2491, 2998, 3380, 4035
AsuII	TTCGAA	2 cut(s)	1555, 1579
AvaI	CYCGRG	3 cut(s)	683, 1767, 4239
AvaII	GGWCC	4 cut(s)	418, 2194, 2464, 4492
BaeGI	GKGCMC	4 cut(s)	42, 98, 1372, 4135
BamHI	GGATCC	1 cut(s)	1009
BanI	GGYRCC	2 cut(s)	37, 1367
BauI	CACGAG	1 cut(s)	3312
BbsI	GAAGAC	1 cut(s)	3079
Bbv12I	GWGCWC	4 cut(s)	98, 2341, 3239, 3308
BbvCI	CCTCAGC	2 cut(s)	843, 4179
BccI	CCATC	7 cut(s)	299, 417, 1880, 2551, 3344, 3415, 4157
BceAI	ACGGC	3 cut(s)	76, 3324, 3558
BcgI	CGANNNNNNTGC	2 cut(s)	1535, 1569
BciT130I	CCWGG	7 cut(s)	606, 663, 1133, 1860, 2211, 2407, 2462
BciVI	GTATCC	4 cut(s)	784, 1798, 1883, 3442
BclI	TGATCA	4 cut(s)	276, 2623, 2644, 2908
BcnI	CCSGG	6 cut(s)	35, 42, 1768, 1769, 2937, 4427
BcoDI	GTCTC	4 cut(s)	2281, 3138, 3655, 4189
BfaI	CTAG	5 cut(s)	746, 891, 2286, 3021, 3036
BfmI	CTRYAG	3 cut(s)	1397, 2037, 4287
BfoI	RGCGCY	4 cut(s)	1801, 3328, 3909, 4456
BfuAI	ACCTGC	1 cut(s)	1699
BfuI	GTATCC	4 cut(s)	784, 1798, 1883, 3442
BglII	AGATCT	3 cut(s)	402, 512, 3440
BlpI	GCTNAGC	2 cut(s)	3059, 4507
Bme18I	GGWCC	4 cut(s)	418, 2194, 2464, 4492
BmeT110I	CYCGRG	3 cut(s)	683, 1767, 4239
BmgT120I	GGNCC	7 cut(s)	142, 418, 965, 2194, 2464, 3252, 4492
BmiI	GGNNCC	4 cut(s)	39, 966, 1011, 1369
BmrI	ACTGGG	2 cut(s)	1625, 3833
BmuI	ACTGGG	2 cut(s)	1625, 3833
BpiI	GAAGAC	1 cut(s)	3079
BplI	GAGNNNNNCTC	2 cut(s)	3295, 3327
BpmI	CTGGAG	6 cut(s)	684, 1074, 2134, 2284, 3723, 3753
Bpu10I	CCTNAGC	3 cut(s)	549, 843, 4179
Bpu1102I	GCTNAGC	2 cut(s)	3059, 4507
Bpu14I	TTCGAA	2 cut(s)	1555, 1579
BpuEI	CTTGAG	3 cut(s)	2334, 2353, 3869
BpuMI	CCSGG	6 cut(s)	35, 42, 1768, 1769, 2937, 4427
BsaAI	YACGTR	2 cut(s)	2117, 4016
BsaBI	GATNNNNATC	2 cut(s)	407, 3227
BsaHI	GRCGYC	1 cut(s)	2000
BsaI	GGTCTC	1 cut(s)	4189
BsaWI	WCCGGW	3 cut(s)	270, 3826, 4045
BsaXI	ACNNNNNCTCC	4 cut(s)	2132, 2162, 3853, 3883
Bsc4I	CCNNNNNNNGG	8 cut(s)	41, 961, 1440, 2443, 2729, 3111, 3785, 4155
Bse118I	RCCGGY	2 cut(s)	1867, 4045
Bse1I	ACTGG	5 cut(s)	319, 1631, 1990, 3839, 3907
Bse3DI	GCAATG	3 cut(s)	1500, 2022, 4365
Bse8I	GATNNNNATC	2 cut(s)	407, 3227
BseBI	CCWGG	7 cut(s)	606, 663, 1133, 1860, 2211, 2407, 2462
BseGI	GGATG	7 cut(s)	2350, 2457, 2812, 2858, 3426, 3596, 4150
BseJI	GATNNNNATC	2 cut(s)	407, 3227
BseLI	CCNNNNNNNGG	8 cut(s)	41, 961, 1440, 2443, 2729, 3111, 3785, 4155
BseMI	GCAATG	3 cut(s)	1500, 2022, 4365
BseNI	ACTGG	5 cut(s)	319, 1631, 1990, 3839, 3907
BseRI	GAGGAG	6 cut(s)	681, 1820, 1839, 2256, 2501, 2516
BseSI	GKGCMC	4 cut(s)	42, 98, 1372, 4135
BseYI	CCCAGC	1 cut(s)	1594
Bsh1236I	CGCG	2 cut(s)	1107, 3399
Bsh1285I	CGRYCG	1 cut(s)	4402
BshFI	GGCC	9 cut(s)	144, 318, 967, 1871, 3053, 3254, 3525, 3778, 4425
BshNI	GGYRCC	2 cut(s)	37, 1367
BshTI	ACCGGT	1 cut(s)	4045
BsiEI	CGRYCG	1 cut(s)	4402
BsiHKAI	GWGCWC	4 cut(s)	98, 2341, 3239, 3308
BsiHKCI	CYCGRG	3 cut(s)	683, 1767, 4239
BslFI	GGGAC	2 cut(s)	666, 1916
BslI	CCNNNNNNNGG	8 cut(s)	41, 961, 1440, 2443, 2729, 3111, 3785, 4155
BsmAI	GTCTC	4 cut(s)	2281, 3138, 3655, 4189
BsmFI	GGGAC	2 cut(s)	666, 1916
BsmI	GAATGC	4 cut(s)	76, 717, 3697, 4519
BsnI	GGCC	9 cut(s)	144, 318, 967, 1871, 3053, 3254, 3525, 3778, 4425
Bso31I	GGTCTC	1 cut(s)	4189
BsoBI	CYCGRG	3 cut(s)	683, 1767, 4239
Bsp119I	TTCGAA	2 cut(s)	1555, 1579
Bsp1286I	GDGCHC	7 cut(s)	42, 98, 1372, 2341, 3239, 3308, 4135
Bsp1407I	TGTACA	2 cut(s)	528, 4352
Bsp1720I	GCTNAGC	2 cut(s)	3059, 4507
Bsp19I	CCATGG	3 cut(s)	866, 1323, 2280
BspANI	GGCC	9 cut(s)	144, 318, 967, 1871, 3053, 3254, 3525, 3778, 4425
BspFNI	CGCG	2 cut(s)	1107, 3399
BspHI	TCATGA	3 cut(s)	381, 1171, 3528
BspLI	GGNNCC	4 cut(s)	39, 966, 1011, 1369
BspMAI	CTGCAG	1 cut(s)	4291
BspMI	ACCTGC	1 cut(s)	1699
BspT104I	TTCGAA	2 cut(s)	1555, 1579
BspT107I	GGYRCC	2 cut(s)	37, 1367
BspTNI	GGTCTC	1 cut(s)	4189
BsrBI	CCGCTC	1 cut(s)	3703
BsrDI	GCAATG	3 cut(s)	1500, 2022, 4365
BsrFI	RCCGGY	2 cut(s)	1867, 4045
BsrGI	TGTACA	2 cut(s)	528, 4352
BsrI	ACTGG	5 cut(s)	319, 1631, 1990, 3839, 3907
BssAI	RCCGGY	2 cut(s)	1867, 4045
BssNAI	GTATAC	2 cut(s)	566, 2314
BssNI	GRCGYC	1 cut(s)	2000
BssSI	CACGAG	1 cut(s)	3312
BssT1I	CCWWGG	5 cut(s)	866, 1323, 1434, 2280, 3106
Bst1107I	GTATAC	2 cut(s)	566, 2314
Bst2BI	CACGAG	1 cut(s)	3312
Bst2UI	CCWGG	7 cut(s)	606, 663, 1133, 1860, 2211, 2407, 2462
Bst6I	CTCTTC	3 cut(s)	1287, 1818, 2662
BstACI	GRCGYC	1 cut(s)	2000
BstAUI	TGTACA	2 cut(s)	528, 4352
BstBAI	YACGTR	2 cut(s)	2117, 4016
BstBI	TTCGAA	2 cut(s)	1555, 1579
BstDSI	CCRYGG	3 cut(s)	866, 1323, 2280
BstENI	CCTNNNNNAGG	1 cut(s)	2441
BstF5I	GGATG	7 cut(s)	2350, 2457, 2812, 2858, 3426, 3596, 4150
BstFNI	CGCG	2 cut(s)	1107, 3399
BstH2I	RGCGCY	4 cut(s)	1801, 3328, 3909, 4456
BstHHI	GCGC	7 cut(s)	1107, 1800, 3327, 3401, 3628, 3908, 4455
BstMAI	GTCTC	4 cut(s)	2281, 3138, 3655, 4189
BstMCI	CGRYCG	1 cut(s)	4402
BstMWI	GCNNNNNNNGC	9 cut(s)	80, 1241, 2345, 2354, 2778, 3407, 3551, 3674, 4289
BstNI	CCWGG	7 cut(s)	606, 663, 1133, 1860, 2211, 2407, 2462
BstNSI	RCATGY	6 cut(s)	229, 695, 837, 1270, 2850, 3925
BstSFI	CTRYAG	3 cut(s)	1397, 2037, 4287
BstSLI	GKGCMC	4 cut(s)	42, 98, 1372, 4135
BstSNI	TACGTA	1 cut(s)	4016
BstUI	CGCG	2 cut(s)	1107, 3399
BstV2I	GAAGAC	1 cut(s)	3079
BstX2I	RGATCY	8 cut(s)	402, 512, 1009, 1681, 2135, 3136, 3344, 3440
BstXI	CCANNNNNNTGG	1 cut(s)	2680
BstYI	RGATCY	8 cut(s)	402, 512, 1009, 1681, 2135, 3136, 3344, 3440
BstZ17I	GTATAC	2 cut(s)	566, 2314
BsuI	GTATCC	4 cut(s)	784, 1798, 1883, 3442
BsuRI	GGCC	9 cut(s)	144, 318, 967, 1871, 3053, 3254, 3525, 3778, 4425
BtgI	CCRYGG	3 cut(s)	866, 1323, 2280
BtsCI	GGATG	7 cut(s)	2350, 2457, 2812, 2858, 3426, 3596, 4150
BtsI	GCAGTG	4 cut(s)	69, 252, 1589, 4251
BveI	ACCTGC	1 cut(s)	1699
CciI	TCATGA	3 cut(s)	381, 1171, 3528
CfoI	GCGC	7 cut(s)	1107, 1800, 3327, 3401, 3628, 3908, 4455
Cfr10I	RCCGGY	2 cut(s)	1867, 4045
Cfr13I	GGNCC	7 cut(s)	142, 418, 965, 2194, 2464, 3252, 4492
Cfr9I	CCCGGG	1 cut(s)	1767
CspAI	ACCGGT	1 cut(s)	4045
CspCI	CAANNNNNGTGG	2 cut(s)	45, 80
DraIII	CACNNNGTG	1 cut(s)	93
DrdI	GACNNNNNNGTC	1 cut(s)	3669
DseDI	GACNNNNNNGTC	1 cut(s)	3669
EaeI	YGGCCR	2 cut(s)	1869, 4423
Eam1104I	CTCTTC	3 cut(s)	1287, 1818, 2662
EarI	CTCTTC	3 cut(s)	1287, 1818, 2662
EciI	GGCGGA	2 cut(s)	181, 915
Eco105I	TACGTA	1 cut(s)	4016
Eco130I	CCWWGG	5 cut(s)	866, 1323, 1434, 2280, 3106
Eco147I	AGGCCT	1 cut(s)	3525
Eco31I	GGTCTC	1 cut(s)	4189
Eco32I	GATATC	2 cut(s)	1948, 3988
Eco47I	GGWCC	4 cut(s)	418, 2194, 2464, 4492
Eco47III	AGCGCT	2 cut(s)	1799, 3326
Eco57I	CTGAAG	5 cut(s)	1098, 1223, 1251, 1842, 3714
Eco88I	CYCGRG	3 cut(s)	683, 1767, 4239
EcoNI	CCTNNNNNAGG	1 cut(s)	2441
EcoO109I	RGGNCCY	1 cut(s)	2194
EcoRI	GAATTC	2 cut(s)	4159, 4436
EcoRII	CCWGG	7 cut(s)	604, 661, 1131, 1858, 2209, 2405, 2460
EcoRV	GATATC	2 cut(s)	1948, 3988
EcoT14I	CCWWGG	5 cut(s)	866, 1323, 1434, 2280, 3106
EcoT22I	ATGCAT	2 cut(s)	649, 1519
ErhI	CCWWGG	5 cut(s)	866, 1323, 1434, 2280, 3106
FaqI	GGGAC	2 cut(s)	666, 1916
FauI	CCCGC	2 cut(s)	2542, 3696
FauNDI	CATATG	1 cut(s)	649
FbaI	TGATCA	4 cut(s)	276, 2623, 2644, 2908
FblI	GTMKAC	4 cut(s)	565, 2295, 2313, 3610
FokI	GGATG	7 cut(s)	2357, 2444, 2819, 2845, 3433, 3583, 4157
FspBI	CTAG	5 cut(s)	746, 891, 2286, 3021, 3036
GlaI	GCGC	7 cut(s)	1106, 1799, 3326, 3400, 3627, 3907, 4454
GsaI	CCCAGC	1 cut(s)	1598
GsuI	CTGGAG	6 cut(s)	684, 1074, 2134, 2284, 3723, 3753
HaeII	RGCGCY	4 cut(s)	1801, 3328, 3909, 4456
HaeIII	GGCC	9 cut(s)	144, 318, 967, 1871, 3053, 3254, 3525, 3778, 4425
HhaI	GCGC	7 cut(s)	1107, 1800, 3327, 3401, 3628, 3908, 4455
Hin1I	GRCGYC	1 cut(s)	2000
Hin6I	GCGC	7 cut(s)	1105, 1798, 3325, 3399, 3626, 3906, 4453
HinP1I	GCGC	7 cut(s)	1105, 1798, 3325, 3399, 3626, 3906, 4453
HincII	GTYRAC	2 cut(s)	1128, 3474
HindII	GTYRAC	2 cut(s)	1128, 3474
HindIII	AAGCTT	3 cut(s)	2779, 4119, 4531
HphI	GGTGA	9 cut(s)	739, 1633, 2000, 2228, 2246, 2491, 2998, 3380, 4035
HpyCH4IV	ACGT	5 cut(s)	582, 2000, 2116, 3817, 4015
HpyF10VI	GCNNNNNNNGC	9 cut(s)	80, 1241, 2345, 2354, 2778, 3407, 3551, 3674, 4289
HpySE526I	ACGT	5 cut(s)	582, 2000, 2116, 3817, 4015
Hsp92I	GRCGYC	1 cut(s)	2000
HspAI	GCGC	7 cut(s)	1105, 1798, 3325, 3399, 3626, 3906, 4453
KroI	GCCGGC	1 cut(s)	1867
KroNI	GCCGGC	1 cut(s)	1869
Ksp22I	TGATCA	4 cut(s)	276, 2623, 2644, 2908
LmnI	GCTCC	9 cut(s)	1306, 1598, 1807, 2026, 2503, 3082, 3169, 3207, 3742
MaeI	CTAG	5 cut(s)	746, 891, 2286, 3021, 3036
MaeII	ACGT	5 cut(s)	582, 2000, 2116, 3817, 4015
MaeIII	GTNAC	9 cut(s)	1025, 1639, 1958, 2001, 2117, 2176, 2416, 2986, 4041
MbiI	CCGCTC	1 cut(s)	3703
MfeI	CAATTG	2 cut(s)	633, 1343
MflI	RGATCY	8 cut(s)	402, 512, 1009, 1681, 2135, 3136, 3344, 3440
MhlI	GDGCHC	7 cut(s)	42, 98, 1372, 2341, 3239, 3308, 4135
MlyI	GAGTC	5 cut(s)	1162, 1898, 2141, 2283, 3326
MmeI	TCCRAC	3 cut(s)	3865, 3874, 4192
Mph1103I	ATGCAT	2 cut(s)	649, 1519
MroNI	GCCGGC	1 cut(s)	1867
MroXI	GAANNNNTTC	3 cut(s)	179, 657, 1236
MslI	CAYNNNNRTG	2 cut(s)	1032, 4101
MspA1I	CMGCKG	5 cut(s)	1142, 2428, 3492, 3743, 4292
MunI	CAATTG	2 cut(s)	633, 1343
Mva1269I	GAATGC	4 cut(s)	76, 717, 3697, 4519
MvaI	CCWGG	7 cut(s)	606, 663, 1133, 1860, 2211, 2407, 2462
MvnI	CGCG	2 cut(s)	1107, 3399
MwoI	GCNNNNNNNGC	9 cut(s)	80, 1241, 2345, 2354, 2778, 3407, 3551, 3674, 4289
NaeI	GCCGGC	1 cut(s)	1869
NciI	CCSGG	6 cut(s)	35, 42, 1768, 1769, 2937, 4427
NcoI	CCATGG	3 cut(s)	866, 1323, 2280
NdeI	CATATG	1 cut(s)	649
NgoMIV	GCCGGC	1 cut(s)	1867
NlaIV	GGNNCC	4 cut(s)	39, 966, 1011, 1369
NmeAIII	GCCGAG	1 cut(s)	283
NmuCI	GTSAC	7 cut(s)	1025, 1639, 2001, 2117, 2176, 2986, 4041
NsiI	ATGCAT	2 cut(s)	649, 1519
NspI	RCATGY	6 cut(s)	229, 695, 837, 1270, 2850, 3925
NspV	TTCGAA	2 cut(s)	1555, 1579
PaeI	GCATGC	1 cut(s)	3925
PaeR7I	CTCGAG	1 cut(s)	4239
PagI	TCATGA	3 cut(s)	381, 1171, 3528
PaqCI	CACCTGC	1 cut(s)	1699
PceI	AGGCCT	1 cut(s)	3525
PciI	ACATGT	2 cut(s)	225, 833
PctI	GAATGC	4 cut(s)	76, 717, 3697, 4519
PdiI	GCCGGC	1 cut(s)	1869
PdmI	GAANNNNTTC	3 cut(s)	179, 657, 1236
PflFI	GACNNNGTC	1 cut(s)	3881
PflMI	CCANNNNNTGG	1 cut(s)	961
PfoI	TCCNGGA	2 cut(s)	661, 1858
PinAI	ACCGGT	1 cut(s)	4045
PleI	GAGTC	5 cut(s)	1162, 1898, 2141, 2282, 3326
PpsI	GAGTC	5 cut(s)	1162, 1898, 2141, 2282, 3326
Ppu21I	YACGTR	2 cut(s)	2117, 4016
PpuMI	RGGWCCY	1 cut(s)	2194
PscI	ACATGT	2 cut(s)	225, 833
PshBI	ATTAAT	1 cut(s)	3432
PsiI	TTATAA	1 cut(s)	2931
Psp5II	RGGWCCY	1 cut(s)	2194
Psp6I	CCWGG	7 cut(s)	604, 661, 1131, 1858, 2209, 2405, 2460
PspFI	CCCAGC	1 cut(s)	1594
PspGI	CCWGG	7 cut(s)	604, 661, 1131, 1858, 2209, 2405, 2460
PspN4I	GGNNCC	4 cut(s)	39, 966, 1011, 1369
PspPI	GGNCC	7 cut(s)	142, 418, 965, 2194, 2464, 3252, 4492
PspPPI	RGGWCCY	1 cut(s)	2194
PstI	CTGCAG	1 cut(s)	4291
PsuI	RGATCY	8 cut(s)	402, 512, 1009, 1681, 2135, 3136, 3344, 3440
PsyI	GACNNNGTC	1 cut(s)	3881
PvuII	CAGCTG	4 cut(s)	1142, 2428, 3743, 4292
RseI	CAYNNNNRTG	2 cut(s)	1032, 4101
Sau96I	GGNCC	7 cut(s)	142, 418, 965, 2194, 2464, 3252, 4492
SchI	GAGTC	5 cut(s)	1162, 1898, 2141, 2283, 3326
SduI	GDGCHC	7 cut(s)	42, 98, 1372, 2341, 3239, 3308, 4135
SfcI	CTRYAG	3 cut(s)	1397, 2037, 4287
Sfr274I	CTCGAG	1 cut(s)	4239
SfuI	TTCGAA	2 cut(s)	1555, 1579
SinI	GGWCC	4 cut(s)	418, 2194, 2464, 4492
SlaI	CTCGAG	1 cut(s)	4239
SmaI	CCCGGG	1 cut(s)	1769
SmiMI	CAYNNNNRTG	2 cut(s)	1032, 4101
SmlI	CTYRAG	4 cut(s)	2332, 2349, 3848, 4239
SmoI	CTYRAG	4 cut(s)	2332, 2349, 3848, 4239
SnaBI	TACGTA	1 cut(s)	4016
SphI	GCATGC	1 cut(s)	3925
SseBI	AGGCCT	1 cut(s)	3525
SspI	AATATT	3 cut(s)	1047, 2251, 3634
SspMI	CTAG	5 cut(s)	746, 891, 2286, 3021, 3036
StuI	AGGCCT	1 cut(s)	3525
StyI	CCWWGG	5 cut(s)	866, 1323, 1434, 2280, 3106
TaiI	ACGT	5 cut(s)	585, 2003, 2119, 3820, 4018
TaqII	GACCGA	1 cut(s)	4416
TatI	WGTACW	5 cut(s)	517, 528, 3655, 4352, 4499
TauI	GCSGC	2 cut(s)	33, 970
TseFI	GTSAC	7 cut(s)	1025, 1639, 2001, 2117, 2176, 2986, 4041
Tsp45I	GTSAC	7 cut(s)	1025, 1639, 2001, 2117, 2176, 2986, 4041
TspGWI	ACGGA	6 cut(s)	401, 457, 611, 1623, 3127, 3494
TspMI	CCCGGG	1 cut(s)	1767
Tth111I	GACNNNGTC	1 cut(s)	3881
Van91I	CCANNNNNTGG	1 cut(s)	961
VneI	GTGCAC	1 cut(s)	94
VpaK11BI	GGWCC	4 cut(s)	418, 2194, 2464, 4492
VspI	ATTAAT	1 cut(s)	3432
XagI	CCTNNNNNAGG	1 cut(s)	2441
XapI	RAATTY	7 cut(s)	285, 298, 1482, 3966, 4159, 4340, 4436
XceI	RCATGY	6 cut(s)	229, 695, 837, 1270, 2850, 3925
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2091
XhoI	CTCGAG	1 cut(s)	4239
XmaI	CCCGGG	1 cut(s)	1767
XmiI	GTMKAC	4 cut(s)	565, 2295, 2313, 3610
XmnI	GAANNNNTTC	3 cut(s)	179, 657, 1236
XspI	CTAG	5 cut(s)	746, 891, 2286, 3021, 3036
ZraI	GACGTC	1 cut(s)	2001
Zsp2I	ATGCAT	2 cut(s)	649, 1519

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.