AT1G30480

glycine rich nucleic binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014235
Physical Location & Seq
Forward (+)
10790022 .. 10792725
2704 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G30480.1

Sequence Viewer

Length: 1164 bp
ATGCTTGGTGGATTATACGGAGATCTTCCTCCACCGACCGATGATGAGAAACCCAGTGGAAACTCCTCTTCCGTCTGGTCAAGCAGTACCAAAATGGCTCCACCTACACTTCGCAAACCACCAGCTTTTGCTCCCCCGCAAACAATCTTAAGACCTCTCAACAAACCTAAACCTATCGTTTCGGCTCCGTACAAGCCTCCTCCTCCTTCGAATTCATCGCAATCGGTGCTTATTCCGGCGAACGAATCAGCACCTTCGCATCAGCCTGCATTGGTTGGTGTGACTTCATCGGTGATTGAAGAGTACGATCCAGCGAGACCTAACGATTACGAGGAGTATAAGAGGGAGAAGAAGAGGAAAGCTACGGAAGCTGAGATGAAACGAGAGATGGATAAGAGAAGGCAAGAGGATGAGGAAAGAGATAAGAGAGAAAGAGAAGAGAGGGAGAAGGAGAGAGAGAGAGATAACAGTGATCCGTCTCGGTTGAATATCTCCGGTGAGGAAGCGTGGAAGAGACGCGCTGCTATGAGTGGTGGTGGAAGTGGTGGGAAGGGAAGATCTTCGTCTCCGCCGGGGAATGTTGATGGGTTTAGTATTGGGAAATCGGAGACGAGTGGGTTAGGAGTAGGAGCAGGTGGACAGATGACAGCTGCTCAGAGGATGATGGCGAAGATGGGATGGAAACAAGGACAAGGGCTTGGGAAATCAGAGCAAGGGATTACTACGCCATTGATGGCTAAGAAGACTGATCGTAGAGCTGGTGTCATTGTCAATGCTAGTGAGAATAAATCTTCTTCGGCGGAGAAGAAGGTTGTTAAGAGTGTTAATATCAATGGTGAACCAACCAGGGTTTTGCTTCTTAGAAACATGGTTGGGCCAGGACAAGTAGATGATGAGCTAGAAGATGAGGTAGGAGGCGAGTGTGGCAAATACGGTACAGTCACTCGTGTACTGATATTCGAGATCACTGAACCGAACTTCCCTGTACACGAAGCAGTAAGAATCTTTGTTCAGTTTTCAAGACCCGAGGAAACAACTAAAGCCCTTGTAGACCTCGATGGGAGATACTTTGGAGGAAGAACCGTACGTGCAACGTTCTATGATGAAGAGAAATTCAGTAAGAACGAGTTGGCTCCAGTTCCAGGTGAAATCCCTGGCTATTAA

Protein Analysis

387

Amino Acids

42.32

Weight (kDa)

8.36

Isoelectric Point (pI)

66.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-patch PF01585 216 - 256 1.6e-17 G-patch domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30480
fragaria_vesca FvH4_3g10030 FvH4_3g10030
malus_domestica MD05G1274000.v1.1 MD10G1253700.v1.1
prunus_persica Prupe.4G089800_v2.0.a1
pyrus_communis pycom05g25540 pycom10g20930
rosa_chinensis RchiOBHm_Chr5g0015891
rosa_laevigata RLG00000032229
rosa_multiflora Rmu_sc0001878.1_g000014
rosa_roxburghii Rroxscaffold_1G00060700
rosa_rugosa Rorug05G0030000
rosa_samantha Rh5AG123600 Rh5BG122500 Rh5CG133900
rosa_wichuraiana Rw5G010670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 623
Acc36I ACCTGC 1 cut(s) 623
AccI GTMKAC 1 cut(s) 1050
AccII CGCG 1 cut(s) 519
AciI CCGC 3 cut(s) 137, 569, 800
AclI AACGTT 1 cut(s) 1094
AclWI GGATC 2 cut(s) 302, 467
AcsI RAATTY 2 cut(s) 211, 1112
AfaI GTAC 7 cut(s) 88, 191, 305, 937, 951, 987, 1086
AfiI CCNNNNNNNGG 1 cut(s) 1142
AflII CTTAAG 1 cut(s) 148
AgsI TTSAA 3 cut(s) 299, 487, 1020
AjnI CCWGG 4 cut(s) 845, 877, 1141, 1153
AloI GAACNNNNNNTCC 2 cut(s) 963, 995
AluBI AGCT 6 cut(s) 125, 362, 371, 650, 758, 898
AluI AGCT 6 cut(s) 125, 362, 371, 650, 758, 898
Alw26I GTCTC 5 cut(s) 310, 483, 508, 570, 602
AlwI GGATC 2 cut(s) 302, 467
Ama87I CYCGRG 1 cut(s) 1025
AoxI GGCC 1 cut(s) 875
ApeKI GCWGC 2 cut(s) 521, 650
ApoI RAATTY 2 cut(s) 211, 1112
Asp700I GAANNNNTTC 1 cut(s) 559
AspLEI GCGC 1 cut(s) 521
AspS9I GGNCC 1 cut(s) 875
AsuC2I CCSGG 1 cut(s) 573
AsuHPI GGTGA 4 cut(s) 304, 509, 848, 1157
AsuII TTCGAA 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 1025
BauI CACGAG 1 cut(s) 945
BbsI GAAGAC 1 cut(s) 749
BbvI GCAGC 2 cut(s) 508, 637
BccI CCATC 7 cut(s) 382, 578, 658, 667, 672, 727, 1052
BciT130I CCWGG 4 cut(s) 847, 879, 1143, 1155
BcnI CCSGG 1 cut(s) 573
BcoDI GTCTC 5 cut(s) 310, 483, 508, 570, 602
BfaI CTAG 2 cut(s) 777, 899
BfrI CTTAAG 1 cut(s) 148
BfuAI ACCTGC 1 cut(s) 623
BglII AGATCT 2 cut(s) 22, 557
BisI GCNGC 2 cut(s) 522, 651
BlsI GCNGC 2 cut(s) 523, 652
Bme1390I CCNGG 5 cut(s) 573, 847, 879, 1143, 1155
BmeT110I CYCGRG 1 cut(s) 1025
BmgT120I GGNCC 1 cut(s) 875
BmiI GGNNCC 3 cut(s) 99, 186, 1134
BmrFI CCNGG 5 cut(s) 573, 847, 879, 1143, 1155
BmrI ACTGGG 1 cut(s) 48
BmsI GCATC 1 cut(s) 268
BmuI ACTGGG 1 cut(s) 48
BpiI GAAGAC 1 cut(s) 749
BpmI CTGGAG 1 cut(s) 1119
Bpu14I TTCGAA 1 cut(s) 209
BpuMI CCSGG 1 cut(s) 573
BsaAI YACGTR 1 cut(s) 1088
BsaI GGTCTC 1 cut(s) 310
BsaJI CCNNGG 4 cut(s) 572, 846, 1026, 1153
BsaWI WCCGGW 1 cut(s) 494
Bsc4I CCNNNNNNNGG 1 cut(s) 1142
Bse1I ACTGG 2 cut(s) 54, 1136
BseBI CCWGG 4 cut(s) 847, 879, 1143, 1155
BseDI CCNNGG 4 cut(s) 572, 846, 1026, 1153
BseGI GGATG 3 cut(s) 415, 666, 683
BseLI CCNNNNNNNGG 1 cut(s) 1142
BseMII CTCAG 2 cut(s) 363, 668
BseNI ACTGG 2 cut(s) 54, 1136
BseRI GAGGAG 4 cut(s) 55, 189, 192, 347
BseXI GCAGC 2 cut(s) 508, 637
Bsh1236I CGCG 1 cut(s) 519
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 1 cut(s) 877
BsiEI CGRYCG 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 1025
BsiSI CCGG 3 cut(s) 236, 495, 572
BsiWI CGTACG 1 cut(s) 1084
BslI CCNNNNNNNGG 1 cut(s) 1142
BsmAI GTCTC 5 cut(s) 310, 483, 508, 570, 602
BsmBI CGTCTC 4 cut(s) 483, 508, 570, 602
BsnI GGCC 1 cut(s) 877
Bso31I GGTCTC 1 cut(s) 310
BsoBI CYCGRG 1 cut(s) 1025
Bsp119I TTCGAA 1 cut(s) 209
Bsp1407I TGTACA 1 cut(s) 985
Bsp143I GATC 6 cut(s) 22, 307, 472, 557, 748, 963
BspACI CCGC 3 cut(s) 137, 569, 800
BspANI GGCC 1 cut(s) 877
BspCNI CTCAG 2 cut(s) 364, 667
BspFNI CGCG 1 cut(s) 519
BspLI GGNNCC 3 cut(s) 99, 186, 1134
BspMI ACCTGC 1 cut(s) 623
BspPI GGATC 2 cut(s) 302, 467
BspT104I TTCGAA 1 cut(s) 209
BspTI CTTAAG 1 cut(s) 148
BspTNI GGTCTC 1 cut(s) 310
BsrGI TGTACA 1 cut(s) 985
BsrI ACTGG 2 cut(s) 54, 1136
BssECI CCNNGG 4 cut(s) 572, 846, 1026, 1153
BssMI GATC 6 cut(s) 22, 307, 472, 557, 748, 963
BssSI CACGAG 1 cut(s) 945
Bst2BI CACGAG 1 cut(s) 945
Bst2UI CCWGG 4 cut(s) 847, 879, 1143, 1155
Bst4CI ACNGT 4 cut(s) 470, 935, 940, 1084
Bst6I CTCTTC 6 cut(s) 73, 294, 347, 432, 506, 1101
BstAFI CTTAAG 1 cut(s) 148
BstAPI GCANNNNNTGC 1 cut(s) 226
BstAUI TGTACA 1 cut(s) 985
BstBAI YACGTR 1 cut(s) 1088
BstBI TTCGAA 1 cut(s) 209
BstC8I GCNNGC 1 cut(s) 267
BstDEI CTNAG 4 cut(s) 372, 654, 738, 860
BstF5I GGATG 3 cut(s) 415, 666, 683
BstFNI CGCG 1 cut(s) 519
BstHHI GCGC 1 cut(s) 521
BstKTI GATC 6 cut(s) 25, 310, 475, 560, 751, 966
BstMAI GTCTC 5 cut(s) 310, 483, 508, 570, 602
BstMBI GATC 6 cut(s) 22, 307, 472, 557, 748, 963
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 3 cut(s) 226, 368, 924
BstNI CCWGG 4 cut(s) 847, 879, 1143, 1155
BstSCI CCNGG 5 cut(s) 571, 845, 877, 1141, 1153
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 2 cut(s) 508, 637
BstV2I GAAGAC 1 cut(s) 749
BstX2I RGATCY 2 cut(s) 22, 557
BstYI RGATCY 2 cut(s) 22, 557
BsuRI GGCC 1 cut(s) 877
BtgZI GCGATG 1 cut(s) 201
BtsCI GGATG 3 cut(s) 415, 666, 683
BtsIMutI CAGTG 3 cut(s) 61, 475, 966
BveI ACCTGC 1 cut(s) 623
Cac8I GCNNGC 1 cut(s) 267
CfoI GCGC 1 cut(s) 521
Cfr13I GGNCC 1 cut(s) 875
CseI GACGC 1 cut(s) 525
Csp6I GTAC 7 cut(s) 87, 190, 304, 936, 950, 986, 1085
CviAII CATG 1 cut(s) 868
CviQI GTAC 7 cut(s) 87, 190, 304, 936, 950, 986, 1085
DdeI CTNAG 4 cut(s) 372, 654, 738, 860
DpnI GATC 6 cut(s) 24, 309, 474, 559, 750, 965
DpnII GATC 6 cut(s) 22, 307, 472, 557, 748, 963
Eam1104I CTCTTC 6 cut(s) 73, 294, 347, 432, 506, 1101
EarI CTCTTC 6 cut(s) 73, 294, 347, 432, 506, 1101
EciI GGCGGA 2 cut(s) 558, 815
Eco31I GGTCTC 1 cut(s) 310
Eco88I CYCGRG 1 cut(s) 1025
EcoRI GAATTC 1 cut(s) 211
EcoRII CCWGG 4 cut(s) 845, 877, 1141, 1153
Esp3I CGTCTC 4 cut(s) 483, 508, 570, 602
FaeI CATG 1 cut(s) 871
FaiI YATR 5 cut(s) 16, 339, 527, 869, 1101
FatI CATG 1 cut(s) 867
FauI CCCGC 1 cut(s) 144
FblI GTMKAC 1 cut(s) 1050
Fnu4HI GCNGC 2 cut(s) 522, 651
FokI GGATG 3 cut(s) 422, 673, 690
Fsp4HI GCNGC 2 cut(s) 522, 651
FspBI CTAG 2 cut(s) 777, 899
GlaI GCGC 1 cut(s) 520
GluI GCNGC 2 cut(s) 522, 651
GsuI CTGGAG 1 cut(s) 1119
HaeIII GGCC 1 cut(s) 877
HapII CCGG 3 cut(s) 236, 495, 572
HgaI GACGC 1 cut(s) 525
HhaI GCGC 1 cut(s) 521
Hin1II CATG 1 cut(s) 871
Hin6I GCGC 1 cut(s) 519
HinP1I GCGC 1 cut(s) 519
HinfI GANTC 2 cut(s) 245, 1002
HpaII CCGG 3 cut(s) 236, 495, 572
HphI GGTGA 4 cut(s) 304, 509, 848, 1157
Hpy166II GTNNAC 5 cut(s) 638, 839, 950, 988, 1051
Hpy188I TCNGA 3 cut(s) 607, 657, 709
Hpy188III TCNNGA 2 cut(s) 961, 1020
Hpy8I GTNNAC 5 cut(s) 638, 839, 950, 988, 1051
HpyAV CCTTC 6 cut(s) 216, 264, 393, 442, 544, 802
HpyCH4III ACNGT 4 cut(s) 470, 935, 940, 1084
HpyCH4IV ACGT 2 cut(s) 1087, 1094
HpyCH4V TGCA 2 cut(s) 269, 1091
HpyF10VI GCNNNNNNNGC 3 cut(s) 226, 368, 924
HpyF3I CTNAG 4 cut(s) 372, 654, 738, 860
HpySE526I ACGT 2 cut(s) 1087, 1094
Hsp92II CATG 1 cut(s) 871
HspAI GCGC 1 cut(s) 519
Kzo9I GATC 6 cut(s) 22, 307, 472, 557, 748, 963
LmnI GCTCC 5 cut(s) 103, 136, 190, 629, 1138
Lsp1109I GCAGC 2 cut(s) 508, 637
LweI GCATC 1 cut(s) 268
MaeI CTAG 2 cut(s) 777, 899
MaeII ACGT 2 cut(s) 1087, 1094
MaeIII GTNAC 2 cut(s) 280, 940
MalI GATC 6 cut(s) 24, 309, 474, 559, 750, 965
MboI GATC 6 cut(s) 22, 307, 472, 557, 748, 963
MflI RGATCY 2 cut(s) 22, 557
MluCI AATT 2 cut(s) 211, 1112
MroXI GAANNNNTTC 1 cut(s) 559
MseI TTAA 4 cut(s) 149, 816, 825, 1162
MspA1I CMGCKG 1 cut(s) 650
MspCI CTTAAG 1 cut(s) 148
MspI CCGG 3 cut(s) 236, 495, 572
MspR9I CCNGG 5 cut(s) 573, 847, 879, 1143, 1155
MvaI CCWGG 4 cut(s) 847, 879, 1143, 1155
MvnI CGCG 1 cut(s) 519
MwoI GCNNNNNNNGC 3 cut(s) 226, 368, 924
NciI CCSGG 1 cut(s) 573
NdeII GATC 6 cut(s) 22, 307, 472, 557, 748, 963
NlaIII CATG 1 cut(s) 871
NlaIV GGNNCC 3 cut(s) 99, 186, 1134
NmuCI GTSAC 2 cut(s) 280, 940
NspV TTCGAA 1 cut(s) 209
PaqCI CACCTGC 1 cut(s) 623
PdmI GAANNNNTTC 1 cut(s) 559
PfeI GAWTC 2 cut(s) 245, 1002
Pfl23II CGTACG 1 cut(s) 1084
PkrI GCNGC 2 cut(s) 523, 652
Ppu21I YACGTR 1 cut(s) 1088
Psp1406I AACGTT 1 cut(s) 1094
Psp6I CCWGG 4 cut(s) 845, 877, 1141, 1153
PspGI CCWGG 4 cut(s) 845, 877, 1141, 1153
PspLI CGTACG 1 cut(s) 1084
PspN4I GGNNCC 3 cut(s) 99, 186, 1134
PspPI GGNCC 1 cut(s) 875
PsuI RGATCY 2 cut(s) 22, 557
PvuII CAGCTG 1 cut(s) 650
RsaI GTAC 7 cut(s) 88, 191, 305, 937, 951, 987, 1086
RsaNI GTAC 7 cut(s) 87, 190, 304, 936, 950, 986, 1085
SaqAI TTAA 4 cut(s) 149, 816, 825, 1162
SatI GCNGC 2 cut(s) 522, 651
Sau3AI GATC 6 cut(s) 22, 307, 472, 557, 748, 963
Sau96I GGNCC 1 cut(s) 875
ScrFI CCNGG 5 cut(s) 573, 847, 879, 1143, 1155
SfaNI GCATC 1 cut(s) 268
SfuI TTCGAA 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse9I AATT 2 cut(s) 211, 1112
SsiI CCGC 3 cut(s) 137, 569, 800
SspMI CTAG 2 cut(s) 777, 899
StyD4I CCNGG 5 cut(s) 571, 845, 877, 1141, 1153
TaaI ACNGT 4 cut(s) 470, 935, 940, 1084
TaiI ACGT 2 cut(s) 1090, 1097
TaqI TCGA 3 cut(s) 209, 960, 1056
TaqII GACCGA 1 cut(s) 53
TasI AATT 2 cut(s) 211, 1112
TatI WGTACW 2 cut(s) 949, 985
TfiI GAWTC 2 cut(s) 245, 1002
Tru1I TTAA 4 cut(s) 149, 816, 825, 1162
Tru9I TTAA 4 cut(s) 149, 816, 825, 1162
TscAI CASTG 3 cut(s) 61, 475, 973
TseFI GTSAC 2 cut(s) 280, 940
TseI GCWGC 2 cut(s) 521, 650
Tsp45I GTSAC 2 cut(s) 280, 940
TspDTI ATGAA 4 cut(s) 204, 276, 392, 1119
TspGWI ACGGA 5 cut(s) 33, 61, 177, 380, 465
TspRI CASTG 3 cut(s) 61, 475, 973
Vha464I CTTAAG 1 cut(s) 148
XapI RAATTY 2 cut(s) 211, 1112
XmiI GTMKAC 1 cut(s) 1050
XmnI GAANNNNTTC 1 cut(s) 559
XspI CTAG 2 cut(s) 777, 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.