Rmu_sc0001878.1_g000014

DNA-damage-repair toleration protein DRT111

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001878.1
OG0014235
Physical Location & Seq
Reverse (-)
66955 .. 69554
2600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001878.1_g000014.1.cds

Sequence Viewer

Length: 1182 bp
atgctaggcgggctatacggcgacctcccgccgccgtcgtcggcggacgaagaaaacaaacccaccaactccacggtgtggtcgagcagcaccaaaatggcgccacagactctccgtaagccctcttcgattttcgcgccaccccagacgatcctcaaatcccagaccaagcccaaaccgtcggcgagctcggcggtttccaaaatcgccttggcgtcgccggtggccgcgccggtgatgcacagcgacgcgtcggtgcagccggcgctggtgggcgtcacgtcgacggtgctggaggagtacgatccggcgaggccgaatgactacgaggagtaccggagggagaggaagaagaaggcgatggaggccgaggtgaggagagagctcgatcggagaagacaggaggaggaggagaaggagaggagagagagggaggagagagaaagagaaagagaaagagagagggagagagatagggatagggatagggatttcggagattcgaggctgaacatttccggcgaggaagcgtggaggcggagggcggcgatgagtggctcagcggcggtgccggaagctccaagatcaccgtctccgccgagcaatgtggatggttttactattgggaagtcggagagtggaggattgggcgtgggagctggaggtcaaatgactgcagctcagagaatgatggcgaaaatggggtggaagcaggggcaggggcttgggaagctagagcaggggattactactcctttgatggccaagaagacggataggagagccggggtgattgtgaatgctagtgaaacaaagccggagaagaaggccaagggtgtcaatcttaatgggccacccactcgggtattgttgcttagaaacatggtgggaccgggtgaggtggatgacgagttagaagatgaagtagcatcagagtgtgcaaagtatggtacagtgacccgggtgctcatatttgagataacagagccaaacttcccagtggatgaagcagtaagaatatttgtgcagtttgagagacctgaagaaacaaccaaagcattggttgatcttgatggtcgatattttggaggtagagtggtccgtgcctcattctatgatgaggagaggtttggtaacaatgaattggctccaatgccaggagaaatccctggtttcacttga

Protein Analysis

393

Amino Acids

43.51

Weight (kDa)

5.77

Isoelectric Point (pI)

63.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30480
fragaria_vesca FvH4_3g10030 FvH4_3g10030
malus_domestica MD05G1274000.v1.1 MD10G1253700.v1.1
prunus_persica Prupe.4G089800_v2.0.a1
pyrus_communis pycom05g25540 pycom10g20930
rosa_chinensis RchiOBHm_Chr5g0015891
rosa_laevigata RLG00000032229
rosa_multiflora Rmu_sc0001878.1_g000014
rosa_roxburghii Rroxscaffold_1G00060700
rosa_rugosa Rorug05G0030000
rosa_samantha Rh5AG123600 Rh5BG122500 Rh5CG133900
rosa_wichuraiana Rw5G010670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 100, 568
AccB7I CCANNNNNTGG 1 cut(s) 78
AccI GTMKAC 1 cut(s) 284
AccII CGCG 3 cut(s) 137, 230, 251
AclWI GGATC 2 cut(s) 145, 299
AcoI YGGCCR 2 cut(s) 225, 762
AcuI CTGAAG 1 cut(s) 1062
AcyI GRCGYC 3 cut(s) 101, 215, 276
AdeI CACNNNGTG 1 cut(s) 78
AfaI GTAC 3 cut(s) 302, 335, 952
AfiI CCNNNNNNNGG 3 cut(s) 78, 375, 1157
AflIII ACRYGT 1 cut(s) 249
AjiI CACGTC 1 cut(s) 282
AjnI CCWGG 2 cut(s) 1156, 1168
AluBI AGCT 6 cut(s) 189, 385, 578, 659, 680, 733
AluI AGCT 6 cut(s) 189, 385, 578, 659, 680, 733
Alw21I GWGCWC 3 cut(s) 191, 387, 969
Alw26I GTCTC 2 cut(s) 597, 1030
AlwI GGATC 2 cut(s) 145, 299
Ama87I CYCGRG 2 cut(s) 861, 960
AoxI GGCC 6 cut(s) 225, 314, 366, 762, 828, 851
ApeKI GCWGC 3 cut(s) 87, 259, 677
AspLEI GCGC 4 cut(s) 103, 139, 232, 268
AspS9I GGNCC 3 cut(s) 851, 890, 1099
AsuC2I CCSGG 4 cut(s) 787, 894, 961, 962
AsuHPI GGTGA 5 cut(s) 247, 385, 579, 802, 908
AvaI CYCGRG 2 cut(s) 861, 960
AvaII GGWCC 2 cut(s) 890, 1099
BalI TGGCCA 1 cut(s) 764
BanI GGYRCC 2 cut(s) 100, 568
BanII GRGCYC 2 cut(s) 191, 387
BarI GAAGNNNNNNTAC 2 cut(s) 109, 141
BbsI GAAGAC 2 cut(s) 403, 776
Bbv12I GWGCWC 3 cut(s) 191, 387, 969
BbvI GCAGC 3 cut(s) 99, 271, 689
BccI CCATC 5 cut(s) 355, 605, 685, 754, 1067
BceAI ACGGC 2 cut(s) 19, 34
BciT130I CCWGG 2 cut(s) 1158, 1170
BcnI CCSGG 4 cut(s) 787, 894, 961, 962
BcoDI GTCTC 2 cut(s) 597, 1030
BfaI CTAG 3 cut(s) 5, 734, 804
BfmI CTRYAG 1 cut(s) 675
BfoI RGCGCY 2 cut(s) 104, 269
BisI GCNGC 7 cut(s) 32, 88, 228, 260, 546, 564, 678
BlpI GCTNAGC 1 cut(s) 559
BlsI GCNGC 7 cut(s) 33, 89, 229, 261, 547, 565, 679
Bme1390I CCNGG 6 cut(s) 787, 894, 961, 962, 1158, 1170
Bme18I GGWCC 2 cut(s) 890, 1099
BmeT110I CYCGRG 2 cut(s) 861, 960
BmgBI CACGTC 1 cut(s) 282
BmgT120I GGNCC 3 cut(s) 851, 890, 1099
BmiI GGNNCC 4 cut(s) 102, 570, 891, 1149
BmrFI CCNGG 6 cut(s) 787, 894, 961, 962, 1158, 1170
BmrI ACTGGG 1 cut(s) 992
BmsI GCATC 2 cut(s) 228, 938
BmuI ACTGGG 1 cut(s) 992
BpiI GAAGAC 2 cut(s) 403, 776
BpmI CTGGAG 2 cut(s) 314, 681
Bpu1102I GCTNAGC 1 cut(s) 559
BpuMI CCSGG 4 cut(s) 787, 894, 961, 962
BsaHI GRCGYC 3 cut(s) 101, 215, 276
BsaI GGTCTC 1 cut(s) 1030
BsaJI CCNNGG 7 cut(s) 72, 210, 369, 786, 831, 960, 1168
BsaWI WCCGGW 1 cut(s) 336
BsaXI ACNNNNNCTCC 4 cut(s) 96, 126, 1080, 1110
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 375, 1157
Bse118I RCCGGY 3 cut(s) 220, 232, 262
Bse1I ACTGG 1 cut(s) 998
Bse3DI GCAATG 1 cut(s) 610
BseBI CCWGG 2 cut(s) 1158, 1170
BseDI CCNNGG 7 cut(s) 72, 210, 369, 786, 831, 960, 1168
BseGI GGATG 3 cut(s) 616, 910, 1009
BseLI CCNNNNNNNGG 3 cut(s) 78, 375, 1157
BseMI GCAATG 1 cut(s) 610
BseMII CTCAG 2 cut(s) 573, 695
BseNI ACTGG 1 cut(s) 998
BseRI GAGGAG 9 cut(s) 311, 344, 391, 419, 422, 425, 436, 449, 1136
BseXI GCAGC 3 cut(s) 99, 271, 689
BsgI GTGCAG 2 cut(s) 278, 1046
Bsh1236I CGCG 3 cut(s) 137, 230, 251
Bsh1285I CGRYCG 1 cut(s) 391
BshFI GGCC 6 cut(s) 227, 316, 368, 764, 830, 853
BshNI GGYRCC 2 cut(s) 100, 568
BsiEI CGRYCG 1 cut(s) 391
BsiHKAI GWGCWC 3 cut(s) 191, 387, 969
BsiHKCI CYCGRG 2 cut(s) 861, 960
BslFI GGGAC 1 cut(s) 903
BslI CCNNNNNNNGG 3 cut(s) 78, 375, 1157
BsmAI GTCTC 2 cut(s) 597, 1030
BsmBI CGTCTC 1 cut(s) 597
BsmFI GGGAC 1 cut(s) 903
BsmI GAATGC 1 cut(s) 805
BsnI GGCC 6 cut(s) 227, 316, 368, 764, 830, 853
Bso31I GGTCTC 1 cut(s) 1030
BsoBI CYCGRG 2 cut(s) 861, 960
Bsp1286I GDGCHC 3 cut(s) 191, 387, 969
Bsp143I GATC 5 cut(s) 150, 304, 388, 584, 1066
Bsp1720I GCTNAGC 1 cut(s) 559
BspANI GGCC 6 cut(s) 227, 316, 368, 764, 830, 853
BspCNI CTCAG 2 cut(s) 572, 694
BspFNI CGCG 3 cut(s) 137, 230, 251
BspLI GGNNCC 4 cut(s) 102, 570, 891, 1149
BspMAI CTGCAG 1 cut(s) 679
BspPI GGATC 2 cut(s) 145, 299
BspT107I GGYRCC 2 cut(s) 100, 568
BspTNI GGTCTC 1 cut(s) 1030
BsrDI GCAATG 1 cut(s) 610
BsrFI RCCGGY 3 cut(s) 220, 232, 262
BsrI ACTGG 1 cut(s) 998
BssAI RCCGGY 3 cut(s) 220, 232, 262
BssECI CCNNGG 7 cut(s) 72, 210, 369, 786, 831, 960, 1168
BssMI GATC 5 cut(s) 150, 304, 388, 584, 1066
BssNI GRCGYC 3 cut(s) 101, 215, 276
BssT1I CCWWGG 2 cut(s) 210, 831
Bst2UI CCWGG 2 cut(s) 1158, 1170
Bst4CI ACNGT 5 cut(s) 76, 180, 289, 591, 955
Bst6I CTCTTC 1 cut(s) 130
BstACI GRCGYC 3 cut(s) 101, 215, 276
BstC8I GCNNGC 3 cut(s) 11, 187, 264
BstDEI CTNAG 3 cut(s) 559, 681, 875
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 3 cut(s) 616, 910, 1009
BstFNI CGCG 3 cut(s) 137, 230, 251
BstH2I RGCGCY 2 cut(s) 104, 269
BstHHI GCGC 4 cut(s) 103, 139, 232, 268
BstKTI GATC 5 cut(s) 153, 307, 391, 587, 1069
BstMAI GTCTC 2 cut(s) 597, 1030
BstMBI GATC 5 cut(s) 150, 304, 388, 584, 1066
BstMCI CGRYCG 1 cut(s) 391
BstMWI GCNNNNNNNGC 6 cut(s) 10, 191, 238, 265, 365, 730
BstNI CCWGG 2 cut(s) 1158, 1170
BstSCI CCNGG 6 cut(s) 785, 892, 959, 960, 1156, 1168
BstSFI CTRYAG 1 cut(s) 675
BstUI CGCG 3 cut(s) 137, 230, 251
BstV1I GCAGC 3 cut(s) 99, 271, 689
BstV2I GAAGAC 2 cut(s) 403, 776
BstXI CCANNNNNNTGG 1 cut(s) 1060
BsuRI GGCC 6 cut(s) 227, 316, 368, 764, 830, 853
BtgI CCRYGG 1 cut(s) 72
BtgZI GCGATG 2 cut(s) 374, 563
BtrI CACGTC 1 cut(s) 282
BtsCI GGATG 3 cut(s) 616, 910, 1009
BtsIMutI CAGTG 2 cut(s) 960, 1005
Cac8I GCNNGC 3 cut(s) 11, 187, 264
CfoI GCGC 4 cut(s) 103, 139, 232, 268
Cfr10I RCCGGY 3 cut(s) 220, 232, 262
Cfr13I GGNCC 3 cut(s) 851, 890, 1099
Cfr9I CCCGGG 1 cut(s) 960
CseI GACGC 4 cut(s) 204, 240, 257, 265
Csp6I GTAC 3 cut(s) 301, 334, 951
CviAII CATG 1 cut(s) 883
CviQI GTAC 3 cut(s) 301, 334, 951
DdeI CTNAG 3 cut(s) 559, 681, 875
DinI GGCGCC 1 cut(s) 102
DpnI GATC 5 cut(s) 152, 306, 390, 586, 1068
DpnII GATC 5 cut(s) 150, 304, 388, 584, 1066
DraIII CACNNNGTG 1 cut(s) 78
EaeI YGGCCR 2 cut(s) 225, 762
Eam1104I CTCTTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 130
EciI GGCGGA 3 cut(s) 59, 553, 585
Ecl136II GAGCTC 2 cut(s) 189, 385
Eco130I CCWWGG 2 cut(s) 210, 831
Eco24I GRGCYC 2 cut(s) 191, 387
Eco31I GGTCTC 1 cut(s) 1030
Eco47I GGWCC 2 cut(s) 890, 1099
Eco53kI GAGCTC 2 cut(s) 189, 385
Eco57I CTGAAG 1 cut(s) 1062
Eco88I CYCGRG 2 cut(s) 861, 960
EcoICRI GAGCTC 2 cut(s) 189, 385
EcoRII CCWGG 2 cut(s) 1156, 1168
EcoT14I CCWWGG 2 cut(s) 210, 831
EcoT38I GRGCYC 2 cut(s) 191, 387
EgeI GGCGCC 1 cut(s) 102
EheI GGCGCC 1 cut(s) 102
ErhI CCWWGG 2 cut(s) 210, 831
Esp3I CGTCTC 1 cut(s) 597
FaeI CATG 1 cut(s) 886
FaiI YATR 5 cut(s) 16, 884, 948, 971, 1116
FaqI GGGAC 1 cut(s) 903
FatI CATG 1 cut(s) 882
FauI CCCGC 2 cut(s) 2, 36
FblI GTMKAC 1 cut(s) 284
Fnu4HI GCNGC 7 cut(s) 32, 88, 228, 260, 546, 564, 678
FokI GGATG 3 cut(s) 623, 917, 1016
FriOI GRGCYC 2 cut(s) 191, 387
Fsp4HI GCNGC 7 cut(s) 32, 88, 228, 260, 546, 564, 678
FspBI CTAG 3 cut(s) 5, 734, 804
GlaI GCGC 4 cut(s) 102, 138, 231, 267
GluI GCNGC 7 cut(s) 32, 88, 228, 260, 546, 564, 678
GsuI CTGGAG 2 cut(s) 314, 681
HaeII RGCGCY 2 cut(s) 104, 269
HaeIII GGCC 6 cut(s) 227, 316, 368, 764, 830, 853
HgaI GACGC 4 cut(s) 204, 240, 257, 265
HhaI GCGC 4 cut(s) 103, 139, 232, 268
Hin1I GRCGYC 3 cut(s) 101, 215, 276
Hin1II CATG 1 cut(s) 886
Hin6I GCGC 4 cut(s) 101, 137, 230, 266
HinP1I GCGC 4 cut(s) 101, 137, 230, 266
HincII GTYRAC 1 cut(s) 285
HindII GTYRAC 1 cut(s) 285
HinfI GANTC 2 cut(s) 109, 500
HphI GGTGA 5 cut(s) 247, 385, 579, 802, 908
Hpy166II GTNNAC 1 cut(s) 285
Hpy188I TCNGA 5 cut(s) 393, 497, 634, 684, 934
Hpy188III TCNNGA 1 cut(s) 1070
Hpy8I GTNNAC 1 cut(s) 285
Hpy99I CGWCG 8 cut(s) 40, 43, 184, 220, 251, 256, 286, 289
HpyAV CCTTC 3 cut(s) 349, 409, 820
HpyCH4III ACNGT 5 cut(s) 76, 180, 289, 591, 955
HpyCH4IV ACGT 1 cut(s) 281
HpyCH4V TGCA 5 cut(s) 241, 259, 677, 941, 1027
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 191, 238, 265, 365, 730
HpyF3I CTNAG 3 cut(s) 559, 681, 875
HpySE526I ACGT 1 cut(s) 281
Hsp92I GRCGYC 3 cut(s) 101, 215, 276
Hsp92II CATG 1 cut(s) 886
HspAI GCGC 4 cut(s) 101, 137, 230, 266
KasI GGCGCC 1 cut(s) 100
KroI GCCGGC 1 cut(s) 262
KroNI GCCGGC 1 cut(s) 264
Kzo9I GATC 5 cut(s) 150, 304, 388, 584, 1066
LmnI GCTCC 3 cut(s) 583, 656, 1153
Lsp1109I GCAGC 3 cut(s) 99, 271, 689
LweI GCATC 2 cut(s) 228, 938
MaeI CTAG 3 cut(s) 5, 734, 804
MaeII ACGT 1 cut(s) 281
MaeIII GTNAC 3 cut(s) 277, 955, 1133
MalI GATC 5 cut(s) 152, 306, 390, 586, 1068
MboI GATC 5 cut(s) 150, 304, 388, 584, 1066
MboII GAAGA 9 cut(s) 62, 117, 361, 364, 408, 781, 835, 929, 1055
MhlI GDGCHC 3 cut(s) 191, 387, 969
MlsI TGGCCA 1 cut(s) 764
MluCI AATT 1 cut(s) 1142
MluI ACGCGT 1 cut(s) 249
MluNI TGGCCA 1 cut(s) 764
Mly113I GGCGCC 1 cut(s) 101
MlyI GAGTC 1 cut(s) 103
MmeI TCCRAC 1 cut(s) 612
Mox20I TGGCCA 1 cut(s) 764
MroNI GCCGGC 1 cut(s) 262
MscI TGGCCA 1 cut(s) 764
MseI TTAA 1 cut(s) 846
MslI CAYNNNNRTG 2 cut(s) 95, 934
Msp20I TGGCCA 1 cut(s) 764
MspA1I CMGCKG 1 cut(s) 563
MspR9I CCNGG 6 cut(s) 787, 894, 961, 962, 1158, 1170
Mva1269I GAATGC 1 cut(s) 805
MvaI CCWGG 2 cut(s) 1158, 1170
MvnI CGCG 3 cut(s) 137, 230, 251
MwoI GCNNNNNNNGC 6 cut(s) 10, 191, 238, 265, 365, 730
NaeI GCCGGC 1 cut(s) 264
NarI GGCGCC 1 cut(s) 101
NciI CCSGG 4 cut(s) 787, 894, 961, 962
NdeII GATC 5 cut(s) 150, 304, 388, 584, 1066
NgoMIV GCCGGC 1 cut(s) 262
NlaIII CATG 1 cut(s) 886
NlaIV GGNNCC 4 cut(s) 102, 570, 891, 1149
NmeAIII GCCGAG 3 cut(s) 170, 394, 624
NmuCI GTSAC 2 cut(s) 277, 955
PcsI WCGNNNNNNNCGW 1 cut(s) 80
PctI GAATGC 1 cut(s) 805
PdiI GCCGGC 1 cut(s) 264
PfeI GAWTC 1 cut(s) 500
PflMI CCANNNNNTGG 1 cut(s) 78
PkrI GCNGC 7 cut(s) 33, 89, 229, 261, 547, 565, 679
Ple19I CGATCG 1 cut(s) 391
PleI GAGTC 1 cut(s) 103
PluTI GGCGCC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 103
Psp124BI GAGCTC 2 cut(s) 191, 387
Psp6I CCWGG 2 cut(s) 1156, 1168
PspGI CCWGG 2 cut(s) 1156, 1168
PspN4I GGNNCC 4 cut(s) 102, 570, 891, 1149
PspPI GGNCC 3 cut(s) 851, 890, 1099
PstI CTGCAG 1 cut(s) 679
PvuI CGATCG 1 cut(s) 391
RsaI GTAC 3 cut(s) 302, 335, 952
RsaNI GTAC 3 cut(s) 301, 334, 951
RseI CAYNNNNRTG 2 cut(s) 95, 934
SacI GAGCTC 2 cut(s) 191, 387
SalI GTCGAC 1 cut(s) 283
SaqAI TTAA 1 cut(s) 846
SatI GCNGC 7 cut(s) 32, 88, 228, 260, 546, 564, 678
Sau3AI GATC 5 cut(s) 150, 304, 388, 584, 1066
Sau96I GGNCC 3 cut(s) 851, 890, 1099
SchI GAGTC 1 cut(s) 103
ScrFI CCNGG 6 cut(s) 787, 894, 961, 962, 1158, 1170
SduI GDGCHC 3 cut(s) 191, 387, 969
SfaNI GCATC 2 cut(s) 228, 938
SfcI CTRYAG 1 cut(s) 675
SfoI GGCGCC 1 cut(s) 102
SgrAI CRCCGGYG 2 cut(s) 220, 232
SgrDI CGTCGACG 1 cut(s) 283
SinI GGWCC 2 cut(s) 890, 1099
SmaI CCCGGG 1 cut(s) 962
SmiMI CAYNNNNRTG 2 cut(s) 95, 934
Sse9I AATT 1 cut(s) 1142
SspDI GGCGCC 1 cut(s) 100
SspI AATATT 1 cut(s) 1020
SspMI CTAG 3 cut(s) 5, 734, 804
SstI GAGCTC 2 cut(s) 191, 387
StyD4I CCNGG 6 cut(s) 785, 892, 959, 960, 1156, 1168
StyI CCWWGG 2 cut(s) 210, 831
TaaI ACNGT 5 cut(s) 76, 180, 289, 591, 955
TaiI ACGT 1 cut(s) 284
TaqI TCGA 6 cut(s) 83, 128, 284, 387, 503, 1078
TasI AATT 1 cut(s) 1142
TauI GCSGC 4 cut(s) 34, 230, 548, 566
TfiI GAWTC 1 cut(s) 500
Tru1I TTAA 1 cut(s) 846
Tru9I TTAA 1 cut(s) 846
TscAI CASTG 2 cut(s) 960, 1005
TseFI GTSAC 2 cut(s) 277, 955
TseI GCWGC 3 cut(s) 87, 259, 677
Tsp45I GTSAC 2 cut(s) 277, 955
TspDTI ATGAA 3 cut(s) 936, 1020, 1155
TspGWI ACGGA 3 cut(s) 104, 788, 1091
TspMI CCCGGG 1 cut(s) 960
TspRI CASTG 2 cut(s) 960, 1005
Van91I CCANNNNNTGG 1 cut(s) 78
VpaK11BI GGWCC 2 cut(s) 890, 1099
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
XmaI CCCGGG 1 cut(s) 960
XmiI GTMKAC 1 cut(s) 284
XspI CTAG 3 cut(s) 5, 734, 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.