pycom05g25540

DNA-damage-repair toleration protein DRT111

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014235
Physical Location & Seq
Reverse (-)
26897775 .. 26901263
3489 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25540.2

Sequence Viewer

Length: 1143 bp
ATGCTAGGTGGGTTGTACGGAGACCTCCCTCCGCCCTCTTCGGCGGAGGACGAGAAGCCCAGCAACAGCACGGTGTGGTCGAGCAGCACGAAAATGGCGCCGCAGACACTCCGGAAGCCAGCGTCGATTTTCGCCCCGCCGCAGACGATTCTAAAGGCACAGAGCAAGCCTAAATCATCGAATTCCGTGCAGCCGAAAATTACAGCGTCACCGGTGGTTGCATTGTCGCCGGCGGTGATACACGATGACGTGGCGCGACCTGAGCTGGTTGGGGTGACGTCGACGGTGCTGGAGGAGTATGATCCGGCGAGGCCCAATGACTATGAGGAGTACAAGAGGGAGAGGAAGAAGAAGGCTATGGAGGCCGAGATGAGGCGGGAACTCGATAGGAGGCGGCAAGAGGAGGAGGAGAAGGAGAGAAGAGAGAAGGAGGAGAGAGAAAGGGAGAGGGAAAGGGATTTTGGAGAATCAAGAAACATTTCGGGTGAGGAAGCCTGGAGGAGGCGTGCGGCGATGAGCGGGGCAGCAACGCCGTCAGTTCCGAGGTCCCCATCTCCGCCGAGTAATCCAGATGGTTTTACCATTGGGAAGTCGGACAGTGGAGGGTTGGGGCTTGGGGCTGGCGGCCAAATGACCGCGGCACAAAGAATGATGGCGAAGATGGGGTGGAAGCAGGGTCAAGGGCTTGGTAGGCAGGAGCAGGGGATTACCACTCCCTTGATGGCCAAGAAAACGGATAGGCGAGCCGGGGTGATTGTGAATGCTAGTGAAACAAAGCCGGAGAAGAAGGTGAAGAGTGTCAGCCTTAACGGGCCACCCACCAGGGTTTTGCTGCTTAGAAACATGGTGGGCCCTGGAGAGGTGGATGATGAATTGGAAGATGAAGTAGCTTCAGAGTGTGCAAAGTATGGGACAGTGACCCGGGTGCTTATATTTGAGATAACAGAGCCAAATTTTCCAGAGAACGAGGCGGTGAGGATCGTTATCCAGTTTGAGAGGTCTGAGGAAACGACTAAAGCATTGGTTGACCTTGATGGTCGGTACTTTGGAGGCAGAGTGGTTCGTGCCACGTTCTATGATGAGGAGAGGTTCGGTAACAACGAGTTGGCTCCAATGCCAGGAGAAATCCCAGGTTTTACTTGA

Protein Analysis

381

Amino Acids

42.04

Weight (kDa)

5.75

Isoelectric Point (pI)

67.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30480
fragaria_vesca FvH4_3g10030 FvH4_3g10030
malus_domestica MD05G1274000.v1.1 MD10G1253700.v1.1
prunus_persica Prupe.4G089800_v2.0.a1
pyrus_communis pycom05g25540 pycom10g20930
rosa_chinensis RchiOBHm_Chr5g0015891
rosa_laevigata RLG00000032229
rosa_multiflora Rmu_sc0001878.1_g000014
rosa_roxburghii Rroxscaffold_1G00060700
rosa_rugosa Rorug05G0030000
rosa_samantha Rh5AG123600 Rh5BG122500 Rh5CG133900
rosa_wichuraiana Rw5G010670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 281
AccB1I GGYRCC 1 cut(s) 97
AccBSI CCGCTC 1 cut(s) 519
AccI GTMKAC 1 cut(s) 281
AccII CGCG 2 cut(s) 256, 638
AccIII TCCGGA 1 cut(s) 111
AclWI GGATC 2 cut(s) 296, 986
AcoI YGGCCR 2 cut(s) 625, 723
AcsI RAATTY 2 cut(s) 181, 952
AcuI CTGAAG 1 cut(s) 876
AcyI GRCGYC 2 cut(s) 98, 278
AdeI CACNNNGTG 1 cut(s) 75
AfaI GTAC 3 cut(s) 17, 332, 1043
AfiI CCNNNNNNNGG 4 cut(s) 372, 501, 859, 1118
AgeI ACCGGT 1 cut(s) 211
AjiI CACGTC 1 cut(s) 250
AjnI CCWGG 5 cut(s) 494, 821, 853, 1117, 1129
AjuI GAANNNNNNNTTGG 2 cut(s) 444, 476
AluBI AGCT 2 cut(s) 265, 890
AluI AGCT 2 cut(s) 265, 890
Alw26I GTCTC 1 cut(s) 15
AlwI GGATC 2 cut(s) 296, 986
Ama87I CYCGRG 1 cut(s) 921
Aor13HI TCCGGA 1 cut(s) 111
AoxI GGCC 6 cut(s) 311, 363, 625, 723, 812, 850
ApaI GGGCCC 1 cut(s) 854
ApeKI GCWGC 4 cut(s) 84, 190, 524, 832
ApoI RAATTY 2 cut(s) 181, 952
AsiGI ACCGGT 1 cut(s) 211
Asp700I GAANNNNTTC 1 cut(s) 478
AspLEI GCGC 2 cut(s) 100, 256
AspS9I GGNCC 5 cut(s) 312, 546, 812, 850, 851
AsuC2I CCSGG 3 cut(s) 748, 922, 923
AsuHPI GGTGA 7 cut(s) 201, 247, 286, 497, 763, 802, 985
AvaI CYCGRG 1 cut(s) 921
AvaII GGWCC 1 cut(s) 546
BaeGI GKGCMC 1 cut(s) 854
BalI TGGCCA 1 cut(s) 725
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 854
BbvI GCAGC 4 cut(s) 96, 202, 536, 819
BccI CCATC 6 cut(s) 559, 566, 646, 655, 715, 1028
BceAI ACGGC 1 cut(s) 517
BcgI CGANNNNNNTGC 2 cut(s) 169, 203
BciT130I CCWGG 5 cut(s) 496, 823, 855, 1119, 1131
BcnI CCSGG 3 cut(s) 748, 922, 923
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 2 cut(s) 5, 765
BfoI RGCGCY 1 cut(s) 101
Bme1390I CCNGG 8 cut(s) 496, 748, 823, 855, 922, 923, 1119, 1131
Bme18I GGWCC 1 cut(s) 546
BmeT110I CYCGRG 1 cut(s) 921
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 5 cut(s) 312, 546, 812, 850, 851
BmiI GGNNCC 4 cut(s) 99, 548, 852, 1110
BmrFI CCNGG 8 cut(s) 496, 748, 823, 855, 922, 923, 1119, 1131
BpmI CTGGAG 3 cut(s) 311, 517, 876
Bpu10I CCTNAGC 1 cut(s) 261
BpuMI CCSGG 3 cut(s) 748, 922, 923
BsaBI GATNNNNATC 1 cut(s) 983
BsaHI GRCGYC 2 cut(s) 98, 278
BsaI GGTCTC 1 cut(s) 15
BsaJI CCNNGG 7 cut(s) 542, 636, 747, 822, 853, 921, 1129
BsaWI WCCGGW 2 cut(s) 111, 211
BsaXI ACNNNNNCTCC 4 cut(s) 490, 520, 1041, 1071
Bsc4I CCNNNNNNNGG 4 cut(s) 372, 501, 859, 1118
Bse118I RCCGGY 2 cut(s) 211, 229
Bse1I ACTGG 1 cut(s) 988
Bse8I GATNNNNATC 1 cut(s) 983
BseAI TCCGGA 1 cut(s) 111
BseBI CCWGG 5 cut(s) 496, 823, 855, 1119, 1131
BseDI CCNNGG 7 cut(s) 542, 636, 747, 822, 853, 921, 1129
BseGI GGATG 1 cut(s) 871
BseJI GATNNNNATC 1 cut(s) 983
BseLI CCNNNNNNNGG 4 cut(s) 372, 501, 859, 1118
BseMII CTCAG 2 cut(s) 252, 993
BseNI ACTGG 1 cut(s) 988
BseRI GAGGAG 8 cut(s) 308, 341, 416, 419, 422, 446, 514, 1097
BseSI GKGCMC 1 cut(s) 854
BseXI GCAGC 4 cut(s) 96, 202, 536, 819
BseYI CCCAGC 1 cut(s) 59
BsgI GTGCAG 1 cut(s) 209
Bsh1236I CGCG 2 cut(s) 256, 638
BshFI GGCC 6 cut(s) 313, 365, 627, 725, 814, 852
BshNI GGYRCC 1 cut(s) 97
BshTI ACCGGT 1 cut(s) 211
BsiHKCI CYCGRG 1 cut(s) 921
BsiSI CCGG 7 cut(s) 112, 212, 230, 305, 747, 779, 922
BslFI GGGAC 2 cut(s) 532, 925
BslI CCNNNNNNNGG 4 cut(s) 372, 501, 859, 1118
BsmAI GTCTC 1 cut(s) 15
BsmFI GGGAC 2 cut(s) 532, 925
BsmI GAATGC 1 cut(s) 766
BsnI GGCC 6 cut(s) 313, 365, 627, 725, 814, 852
Bso31I GGTCTC 1 cut(s) 15
BsoBI CYCGRG 1 cut(s) 921
Bsp120I GGGCCC 1 cut(s) 850
Bsp1286I GDGCHC 1 cut(s) 854
Bsp13I TCCGGA 1 cut(s) 111
Bsp143I GATC 2 cut(s) 301, 978
BspANI GGCC 6 cut(s) 313, 365, 627, 725, 814, 852
BspCNI CTCAG 2 cut(s) 253, 994
BspEI TCCGGA 1 cut(s) 111
BspFNI CGCG 2 cut(s) 256, 638
BspLI GGNNCC 4 cut(s) 99, 548, 852, 1110
BspPI GGATC 2 cut(s) 296, 986
BspT107I GGYRCC 1 cut(s) 97
BspTNI GGTCTC 1 cut(s) 15
BsrBI CCGCTC 1 cut(s) 519
BsrFI RCCGGY 2 cut(s) 211, 229
BsrI ACTGG 1 cut(s) 988
BssAI RCCGGY 2 cut(s) 211, 229
BssECI CCNNGG 7 cut(s) 542, 636, 747, 822, 853, 921, 1129
BssMI GATC 2 cut(s) 301, 978
BssNI GRCGYC 2 cut(s) 98, 278
Bst2UI CCWGG 5 cut(s) 496, 823, 855, 1119, 1131
Bst4CI ACNGT 4 cut(s) 73, 286, 599, 916
Bst6I CTCTTC 3 cut(s) 43, 415, 788
BstACI GRCGYC 2 cut(s) 98, 278
BstC8I GCNNGC 6 cut(s) 120, 167, 231, 507, 622, 744
BstDEI CTNAG 3 cut(s) 261, 836, 1002
BstDSI CCRYGG 1 cut(s) 636
BstENI CCTNNNNNAGG 1 cut(s) 499
BstF5I GGATG 1 cut(s) 871
BstFNI CGCG 2 cut(s) 256, 638
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 2 cut(s) 100, 256
BstKTI GATC 2 cut(s) 304, 981
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 2 cut(s) 301, 978
BstMWI GCNNNNNNNGC 3 cut(s) 262, 362, 691
BstNI CCWGG 5 cut(s) 496, 823, 855, 1119, 1131
BstSCI CCNGG 8 cut(s) 494, 746, 821, 853, 920, 921, 1117, 1129
BstSLI GKGCMC 1 cut(s) 854
BstUI CGCG 2 cut(s) 256, 638
BstV1I GCAGC 4 cut(s) 96, 202, 536, 819
BsuRI GGCC 6 cut(s) 313, 365, 627, 725, 814, 852
BtgI CCRYGG 1 cut(s) 636
BtgZI GCGATG 1 cut(s) 527
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 1 cut(s) 871
BtsIMutI CAGTG 2 cut(s) 604, 921
Cac8I GCNNGC 6 cut(s) 120, 167, 231, 507, 622, 744
CfoI GCGC 2 cut(s) 100, 256
Cfr10I RCCGGY 2 cut(s) 211, 229
Cfr13I GGNCC 5 cut(s) 312, 546, 812, 850, 851
Cfr42I CCGCGG 1 cut(s) 639
Cfr9I CCCGGG 1 cut(s) 921
CseI GACGC 2 cut(s) 111, 195
Csp6I GTAC 3 cut(s) 16, 331, 1042
CspAI ACCGGT 1 cut(s) 211
CviAII CATG 1 cut(s) 844
CviQI GTAC 3 cut(s) 16, 331, 1042
DdeI CTNAG 3 cut(s) 261, 836, 1002
DinI GGCGCC 1 cut(s) 99
DpnI GATC 2 cut(s) 303, 980
DpnII GATC 2 cut(s) 301, 978
DraIII CACNNNGTG 1 cut(s) 75
EaeI YGGCCR 2 cut(s) 625, 723
Eam1104I CTCTTC 3 cut(s) 43, 415, 788
EarI CTCTTC 3 cut(s) 43, 415, 788
EciI GGCGGA 3 cut(s) 21, 59, 546
Eco24I GRGCYC 1 cut(s) 854
Eco31I GGTCTC 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 546
Eco57I CTGAAG 1 cut(s) 876
Eco88I CYCGRG 1 cut(s) 921
EcoNI CCTNNNNNAGG 1 cut(s) 499
EcoO109I RGGNCCY 2 cut(s) 546, 851
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 5 cut(s) 494, 821, 853, 1117, 1129
EcoT38I GRGCYC 1 cut(s) 854
EgeI GGCGCC 1 cut(s) 99
EheI GGCGCC 1 cut(s) 99
FaeI CATG 1 cut(s) 847
FaiI YATR 7 cut(s) 300, 324, 359, 845, 909, 932, 1077
FaqI GGGAC 2 cut(s) 532, 925
FatI CATG 1 cut(s) 843
FauI CCCGC 3 cut(s) 144, 369, 512
FblI GTMKAC 1 cut(s) 281
FokI GGATG 1 cut(s) 878
FriOI GRGCYC 1 cut(s) 854
FspBI CTAG 2 cut(s) 5, 765
GlaI GCGC 2 cut(s) 99, 255
GsaI CCCAGC 1 cut(s) 63
GsuI CTGGAG 3 cut(s) 311, 517, 876
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 6 cut(s) 313, 365, 627, 725, 814, 852
HapII CCGG 7 cut(s) 112, 212, 230, 305, 747, 779, 922
HgaI GACGC 2 cut(s) 111, 195
HhaI GCGC 2 cut(s) 100, 256
Hin1I GRCGYC 2 cut(s) 98, 278
Hin1II CATG 1 cut(s) 847
Hin6I GCGC 2 cut(s) 98, 254
HinP1I GCGC 2 cut(s) 98, 254
HincII GTYRAC 2 cut(s) 282, 1027
HindII GTYRAC 2 cut(s) 282, 1027
HinfI GANTC 2 cut(s) 148, 467
HpaII CCGG 7 cut(s) 112, 212, 230, 305, 747, 779, 922
HphI GGTGA 7 cut(s) 201, 247, 286, 497, 763, 802, 985
Hpy166II GTNNAC 2 cut(s) 282, 1027
Hpy188I TCNGA 4 cut(s) 543, 595, 895, 1003
Hpy188III TCNNGA 4 cut(s) 112, 471, 569, 959
Hpy8I GTNNAC 2 cut(s) 282, 1027
Hpy99I CGWCG 3 cut(s) 127, 283, 286
HpyAV CCTTC 4 cut(s) 346, 406, 421, 781
HpyCH4III ACNGT 4 cut(s) 73, 286, 599, 916
HpyCH4IV ACGT 3 cut(s) 249, 278, 1070
HpyCH4V TGCA 3 cut(s) 190, 221, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 262, 362, 691
HpyF3I CTNAG 3 cut(s) 261, 836, 1002
HpySE526I ACGT 3 cut(s) 249, 278, 1070
Hsp92I GRCGYC 2 cut(s) 98, 278
Hsp92II CATG 1 cut(s) 847
HspAI GCGC 2 cut(s) 98, 254
KasI GGCGCC 1 cut(s) 97
Kpn2I TCCGGA 1 cut(s) 111
KroI GCCGGC 1 cut(s) 229
KroNI GCCGGC 1 cut(s) 231
KspI CCGCGG 1 cut(s) 639
Kzo9I GATC 2 cut(s) 301, 978
LmnI GCTCC 2 cut(s) 697, 1114
Lsp1109I GCAGC 4 cut(s) 96, 202, 536, 819
MaeI CTAG 2 cut(s) 5, 765
MaeII ACGT 3 cut(s) 249, 278, 1070
MaeIII GTNAC 4 cut(s) 207, 274, 916, 1094
MalI GATC 2 cut(s) 303, 980
MbiI CCGCTC 1 cut(s) 519
MboI GATC 2 cut(s) 301, 978
MboII GAAGA 8 cut(s) 30, 358, 361, 432, 670, 796, 805, 890
MhlI GDGCHC 1 cut(s) 854
MlsI TGGCCA 1 cut(s) 725
MluCI AATT 4 cut(s) 181, 198, 872, 952
MluNI TGGCCA 1 cut(s) 725
Mly113I GGCGCC 1 cut(s) 98
MmeI TCCRAC 1 cut(s) 573
Mox20I TGGCCA 1 cut(s) 725
MreI CGCCGGCG 1 cut(s) 229
MroI TCCGGA 1 cut(s) 111
MroNI GCCGGC 1 cut(s) 229
MroXI GAANNNNTTC 1 cut(s) 478
MscI TGGCCA 1 cut(s) 725
MseI TTAA 1 cut(s) 807
MslI CAYNNNNRTG 1 cut(s) 92
Msp20I TGGCCA 1 cut(s) 725
MspA1I CMGCKG 1 cut(s) 638
MspI CCGG 7 cut(s) 112, 212, 230, 305, 747, 779, 922
MspR9I CCNGG 8 cut(s) 496, 748, 823, 855, 922, 923, 1119, 1131
Mva1269I GAATGC 1 cut(s) 766
MvaI CCWGG 5 cut(s) 496, 823, 855, 1119, 1131
MvnI CGCG 2 cut(s) 256, 638
MwoI GCNNNNNNNGC 3 cut(s) 262, 362, 691
NaeI GCCGGC 1 cut(s) 231
NarI GGCGCC 1 cut(s) 98
NciI CCSGG 3 cut(s) 748, 922, 923
NdeII GATC 2 cut(s) 301, 978
NgoMIV GCCGGC 1 cut(s) 229
NlaIII CATG 1 cut(s) 847
NlaIV GGNNCC 4 cut(s) 99, 548, 852, 1110
NmeAIII GCCGAG 2 cut(s) 391, 585
NmuCI GTSAC 3 cut(s) 207, 274, 916
PcsI WCGNNNNNNNCGW 3 cut(s) 77, 86, 1098
PctI GAATGC 1 cut(s) 766
PdiI GCCGGC 1 cut(s) 231
PdmI GAANNNNTTC 1 cut(s) 478
PfeI GAWTC 2 cut(s) 148, 467
PinAI ACCGGT 1 cut(s) 211
PluTI GGCGCC 1 cut(s) 101
PpuMI RGGWCCY 1 cut(s) 546
Psp5II RGGWCCY 1 cut(s) 546
Psp6I CCWGG 5 cut(s) 494, 821, 853, 1117, 1129
PspFI CCCAGC 1 cut(s) 59
PspGI CCWGG 5 cut(s) 494, 821, 853, 1117, 1129
PspN4I GGNNCC 4 cut(s) 99, 548, 852, 1110
PspOMI GGGCCC 1 cut(s) 850
PspPI GGNCC 5 cut(s) 312, 546, 812, 850, 851
PspPPI RGGWCCY 1 cut(s) 546
RsaI GTAC 3 cut(s) 17, 332, 1043
RsaNI GTAC 3 cut(s) 16, 331, 1042
RseI CAYNNNNRTG 1 cut(s) 92
SacII CCGCGG 1 cut(s) 639
SalI GTCGAC 1 cut(s) 280
SaqAI TTAA 1 cut(s) 807
Sau3AI GATC 2 cut(s) 301, 978
Sau96I GGNCC 5 cut(s) 312, 546, 812, 850, 851
ScrFI CCNGG 8 cut(s) 496, 748, 823, 855, 922, 923, 1119, 1131
SduI GDGCHC 1 cut(s) 854
SfoI GGCGCC 1 cut(s) 99
Sfr303I CCGCGG 1 cut(s) 639
SgrAI CRCCGGYG 2 cut(s) 211, 229
SgrBI CCGCGG 1 cut(s) 639
SgrDI CGTCGACG 1 cut(s) 280
SinI GGWCC 1 cut(s) 546
SmaI CCCGGG 1 cut(s) 923
SmiMI CAYNNNNRTG 1 cut(s) 92
Sse9I AATT 4 cut(s) 181, 198, 872, 952
SspDI GGCGCC 1 cut(s) 97
SspMI CTAG 2 cut(s) 5, 765
StyD4I CCNGG 8 cut(s) 494, 746, 821, 853, 920, 921, 1117, 1129
TaaI ACNGT 4 cut(s) 73, 286, 599, 916
TaiI ACGT 3 cut(s) 252, 281, 1073
TaqI TCGA 5 cut(s) 80, 125, 179, 281, 384
TasI AATT 4 cut(s) 181, 198, 872, 952
TatI WGTACW 1 cut(s) 330
TauI GCSGC 6 cut(s) 103, 142, 397, 512, 627, 641
TfiI GAWTC 2 cut(s) 148, 467
Tru1I TTAA 1 cut(s) 807
Tru9I TTAA 1 cut(s) 807
TscAI CASTG 2 cut(s) 604, 921
TseFI GTSAC 3 cut(s) 207, 274, 916
TseI GCWGC 4 cut(s) 84, 190, 524, 832
Tsp45I GTSAC 3 cut(s) 207, 274, 916
TspDTI ATGAA 2 cut(s) 885, 897
TspGWI ACGGA 3 cut(s) 33, 175, 749
TspMI CCCGGG 1 cut(s) 921
TspRI CASTG 2 cut(s) 604, 921
VpaK11BI GGWCC 1 cut(s) 546
XagI CCTNNNNNAGG 1 cut(s) 499
XapI RAATTY 2 cut(s) 181, 952
XcmI CCANNNNNNNNNTGG 1 cut(s) 718
XmaI CCCGGG 1 cut(s) 921
XmiI GTMKAC 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 478
XspI CTAG 2 cut(s) 5, 765
ZraI GACGTC 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.