Prupe.4G089800_v2.0.a1

DNA-damage-repair toleration protein DRT111

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0014235
Physical Location & Seq
Forward (+)
4468429 .. 4472620
4192 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G089800.1

Sequence Viewer

Length: 1149 bp
ATGCTGGGTGGATTGTACGGAGACCTCCCTCCACCGTCTTCGGCGGAGGAAGAGAAACCCAGCAACACCACGGTGTGGTCGAGCAGCACCAAAATGGCGCCGCAGACTCTCCGAAAACCCGCATCCGTTTTCGCTCCGCCGCAGACGATTCTCAAGTCCCAAAACAAGCCCAAGCCTTCGAATTTAGCCCAGCCCAAAATTACAGCATCACCGGTAATTGCGACGTCGGTGATACAAAATGACATGGTGCAACCTGCCCTGGTGGGGGTCACGTCGACGGTGCTAGAGGAGTATGATCCGGCGAGGCCCAATGACTACGAGGAGTACAAGAGGGAGAGGAAGAAGAAGGCAATGGAGGCTGAGATGAGGCGAGAACTCGATAGGAGGCGGCAAGAGGAGGAGGAGAAGGAGAGGAGAGAGAGGGAGGAGAGAGAAAGGGAAAGGGAGAGGGATTTTGGAGATTCAAGGTTGAATATTTCGGGTGAAGAAGCCTGGAGGAGGCGTGCGGCTATGAGCGGCGGGGCAGTTCCAGCAATGCCGGCAATTCCAAGGTCACCGTCTCCTCCTAATAATGGAGATGGTTTTACCATTGGGAAGTCGGAGAGTGGCGGGTTGGGGTTAGGGGCTGGCGGCCAAATGACCGCAGCACAGAGAATGATGGCGAAGATGGGGTGGAAGCAGGGGCAAGGGCTTGGGAAGCAGGAGCAGGGGATCACTACTCCCTTGATGGCCAAGAAAACGGATAGGAGAGCCGGGGTGATTGTGAATGCCAGTGAAACAAAGCCGGAGAAGAAGGCGAAGAGTGTCAGCCTTAATGGGCCACCCACCAGGGTTTTGCTGCTTAGAAACATGGTGGGCCCTGGCGAGGTGGATGATGAGTTGGAAGATGAGGTAGCATCAGAGTGTGCAAAGTATGGGACAGTGACCCGGGTGCTTATATTTGAGATAACGGAGCCAAATTTCCCGGTGGATGAGGCAGTGAGAATCTTTGTGCAGTTTGAGAGGTCTGAAGAAACGACTAAAGCATTGGTCGACCTTGATGGTCGATACTTTGGAGGTAGGGTGGTTCGCGCCTCGTTCTATGACGAGGAGAGGTTCGGCAACAACGAGCTGGCTCCAATGCCAGGAGAAATTCCCGGGTTCACTTGA

Protein Analysis

383

Amino Acids

42.24

Weight (kDa)

5.81

Isoelectric Point (pI)

66.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30480
fragaria_vesca FvH4_3g10030 FvH4_3g10030
malus_domestica MD05G1274000.v1.1 MD10G1253700.v1.1
prunus_persica Prupe.4G089800_v2.0.a1
pyrus_communis pycom05g25540 pycom10g20930
rosa_chinensis RchiOBHm_Chr5g0015891
rosa_laevigata RLG00000032229
rosa_multiflora Rmu_sc0001878.1_g000014
rosa_roxburghii Rroxscaffold_1G00060700
rosa_rugosa Rorug05G0030000
rosa_samantha Rh5AG123600 Rh5BG122500 Rh5CG133900
rosa_wichuraiana Rw5G010670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 227
Acc36I ACCTGC 1 cut(s) 262
AccB1I GGYRCC 1 cut(s) 97
AccB7I CCANNNNNTGG 1 cut(s) 75
AccBSI CCGCTC 1 cut(s) 516
AccI GTMKAC 2 cut(s) 275, 1032
AccII CGCG 1 cut(s) 1071
AclWI GGATC 2 cut(s) 290, 719
AcoI YGGCCR 2 cut(s) 631, 729
AcsI RAATTY 3 cut(s) 181, 958, 1131
AcuI CTGAAG 1 cut(s) 1029
AcyI GRCGYC 2 cut(s) 98, 224
AdeI CACNNNGTG 1 cut(s) 75
AfaI GTAC 2 cut(s) 17, 326
AfiI CCNNNNNNNGG 7 cut(s) 75, 264, 265, 498, 572, 865, 1124
AgeI ACCGGT 1 cut(s) 211
AgsI TTSAA 2 cut(s) 465, 472
AjiI CACGTC 1 cut(s) 273
AjnI CCWGG 5 cut(s) 258, 491, 827, 859, 1123
AleI CACNNNNGTG 1 cut(s) 71
AluBI AGCT 1 cut(s) 1111
AluI AGCT 1 cut(s) 1111
Alw26I GTCTC 2 cut(s) 15, 564
AlwI GGATC 2 cut(s) 290, 719
Ama87I CYCGRG 2 cut(s) 927, 1136
AoxI GGCC 5 cut(s) 305, 631, 729, 818, 856
ApaI GGGCCC 1 cut(s) 860
ApeKI GCWGC 3 cut(s) 84, 644, 838
ApoI RAATTY 3 cut(s) 181, 958, 1131
AsiGI ACCGGT 1 cut(s) 211
AspLEI GCGC 2 cut(s) 100, 1073
AspS9I GGNCC 4 cut(s) 306, 818, 856, 857
AsuC2I CCSGG 6 cut(s) 754, 928, 929, 965, 1137, 1138
AsuHPI GGTGA 5 cut(s) 201, 241, 494, 546, 769
AsuII TTCGAA 1 cut(s) 179
AvaI CYCGRG 2 cut(s) 927, 1136
BaeGI GKGCMC 1 cut(s) 860
BalI TGGCCA 1 cut(s) 731
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 860
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 3 cut(s) 96, 656, 825
BccI CCATC 5 cut(s) 572, 652, 661, 721, 1034
BciT130I CCWGG 5 cut(s) 260, 493, 829, 861, 1125
BcnI CCSGG 6 cut(s) 754, 928, 929, 965, 1137, 1138
BcoDI GTCTC 2 cut(s) 15, 564
BfaI CTAG 1 cut(s) 284
BfoI RGCGCY 1 cut(s) 101
BfuAI ACCTGC 1 cut(s) 262
BisI GCNGC 9 cut(s) 85, 101, 140, 389, 507, 517, 631, 645, 839
BlsI GCNGC 9 cut(s) 86, 102, 141, 390, 508, 518, 632, 646, 840
BmeT110I CYCGRG 2 cut(s) 927, 1136
BmgBI CACGTC 1 cut(s) 273
BmgT120I GGNCC 4 cut(s) 306, 818, 856, 857
BmiI GGNNCC 4 cut(s) 99, 858, 954, 1116
BmsI GCATC 3 cut(s) 131, 215, 905
BpiI GAAGAC 1 cut(s) 30
BpmI CTGGAG 1 cut(s) 514
Bpu14I TTCGAA 1 cut(s) 179
BpuEI CTTGAG 1 cut(s) 137
BpuMI CCSGG 6 cut(s) 754, 928, 929, 965, 1137, 1138
BsaHI GRCGYC 2 cut(s) 98, 224
BsaI GGTCTC 1 cut(s) 15
BsaJI CCNNGG 8 cut(s) 69, 258, 548, 753, 828, 859, 927, 1136
BsaWI WCCGGW 1 cut(s) 211
BsaXI ACNNNNNCTCC 4 cut(s) 487, 517, 1047, 1077
Bsc4I CCNNNNNNNGG 7 cut(s) 75, 264, 265, 498, 572, 865, 1124
Bse118I RCCGGY 2 cut(s) 211, 538
Bse1I ACTGG 1 cut(s) 771
Bse3DI GCAATG 2 cut(s) 357, 540
BseBI CCWGG 5 cut(s) 260, 493, 829, 861, 1125
BseDI CCNNGG 8 cut(s) 69, 258, 548, 753, 828, 859, 927, 1136
BseGI GGATG 3 cut(s) 122, 877, 976
BseLI CCNNNNNNNGG 7 cut(s) 75, 264, 265, 498, 572, 865, 1124
BseMI GCAATG 2 cut(s) 357, 540
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 1 cut(s) 771
BseSI GKGCMC 1 cut(s) 860
BseXI GCAGC 3 cut(s) 96, 656, 825
BseYI CCCAGC 3 cut(s) 4, 59, 189
BsgI GTGCAG 1 cut(s) 1013
Bsh1236I CGCG 1 cut(s) 1071
BshFI GGCC 5 cut(s) 307, 633, 731, 820, 858
BshNI GGYRCC 1 cut(s) 97
BshTI ACCGGT 1 cut(s) 211
BsiHKCI CYCGRG 2 cut(s) 927, 1136
BsiSI CCGG 8 cut(s) 212, 299, 539, 753, 785, 928, 965, 1137
BslFI GGGAC 2 cut(s) 142, 931
BslI CCNNNNNNNGG 7 cut(s) 75, 264, 265, 498, 572, 865, 1124
BsmAI GTCTC 2 cut(s) 15, 564
BsmBI CGTCTC 1 cut(s) 564
BsmFI GGGAC 2 cut(s) 142, 931
BsmI GAATGC 1 cut(s) 772
BsnI GGCC 5 cut(s) 307, 633, 731, 820, 858
Bso31I GGTCTC 1 cut(s) 15
BsoBI CYCGRG 2 cut(s) 927, 1136
Bsp119I TTCGAA 1 cut(s) 179
Bsp120I GGGCCC 1 cut(s) 856
Bsp1286I GDGCHC 1 cut(s) 860
Bsp143I GATC 2 cut(s) 295, 711
BspANI GGCC 5 cut(s) 307, 633, 731, 820, 858
BspCNI CTCAG 1 cut(s) 352
BspFNI CGCG 1 cut(s) 1071
BspLI GGNNCC 4 cut(s) 99, 858, 954, 1116
BspMI ACCTGC 1 cut(s) 262
BspPI GGATC 2 cut(s) 290, 719
BspT104I TTCGAA 1 cut(s) 179
BspT107I GGYRCC 1 cut(s) 97
BspTNI GGTCTC 1 cut(s) 15
BsrBI CCGCTC 1 cut(s) 516
BsrDI GCAATG 2 cut(s) 357, 540
BsrFI RCCGGY 2 cut(s) 211, 538
BsrI ACTGG 1 cut(s) 771
BssAI RCCGGY 2 cut(s) 211, 538
BssECI CCNNGG 8 cut(s) 69, 258, 548, 753, 828, 859, 927, 1136
BssMI GATC 2 cut(s) 295, 711
BssNI GRCGYC 2 cut(s) 98, 224
BssT1I CCWWGG 1 cut(s) 548
Bst2UI CCWGG 5 cut(s) 260, 493, 829, 861, 1125
Bst4CI ACNGT 5 cut(s) 36, 73, 280, 558, 922
Bst6I CTCTTC 2 cut(s) 45, 794
BstACI GRCGYC 2 cut(s) 98, 224
BstBI TTCGAA 1 cut(s) 179
BstC8I GCNNGC 4 cut(s) 504, 540, 628, 1113
BstDEI CTNAG 2 cut(s) 360, 842
BstDSI CCRYGG 1 cut(s) 69
BstEII GGTNACC 1 cut(s) 552
BstENI CCTNNNNNAGG 1 cut(s) 496
BstF5I GGATG 3 cut(s) 122, 877, 976
BstFNI CGCG 1 cut(s) 1071
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 2 cut(s) 100, 1073
BstKTI GATC 2 cut(s) 298, 714
BstMAI GTCTC 2 cut(s) 15, 564
BstMBI GATC 2 cut(s) 295, 711
BstMWI GCNNNNNNNGC 4 cut(s) 356, 530, 539, 697
BstNI CCWGG 5 cut(s) 260, 493, 829, 861, 1125
BstPI GGTNACC 1 cut(s) 552
BstSLI GKGCMC 1 cut(s) 860
BstUI CGCG 1 cut(s) 1071
BstV1I GCAGC 3 cut(s) 96, 656, 825
BstV2I GAAGAC 1 cut(s) 30
BsuRI GGCC 5 cut(s) 307, 633, 731, 820, 858
BtgI CCRYGG 1 cut(s) 69
BtrI CACGTC 1 cut(s) 273
BtsCI GGATG 3 cut(s) 122, 877, 976
BtsI GCAGTG 1 cut(s) 984
BtsIMutI CAGTG 3 cut(s) 778, 927, 984
BveI ACCTGC 1 cut(s) 262
Cac8I GCNNGC 4 cut(s) 504, 540, 628, 1113
CfoI GCGC 2 cut(s) 100, 1073
Cfr10I RCCGGY 2 cut(s) 211, 538
Cfr13I GGNCC 4 cut(s) 306, 818, 856, 857
Cfr9I CCCGGG 2 cut(s) 927, 1136
Csp6I GTAC 2 cut(s) 16, 325
CspAI ACCGGT 1 cut(s) 211
CviAII CATG 2 cut(s) 244, 850
CviQI GTAC 2 cut(s) 16, 325
DdeI CTNAG 2 cut(s) 360, 842
DinI GGCGCC 1 cut(s) 99
DpnI GATC 2 cut(s) 297, 713
DpnII GATC 2 cut(s) 295, 711
DraIII CACNNNGTG 1 cut(s) 75
EaeI YGGCCR 2 cut(s) 631, 729
Eam1104I CTCTTC 2 cut(s) 45, 794
EarI CTCTTC 2 cut(s) 45, 794
EciI GGCGGA 2 cut(s) 59, 126
Eco130I CCWWGG 1 cut(s) 548
Eco24I GRGCYC 1 cut(s) 860
Eco31I GGTCTC 1 cut(s) 15
Eco57I CTGAAG 1 cut(s) 1029
Eco88I CYCGRG 2 cut(s) 927, 1136
Eco91I GGTNACC 1 cut(s) 552
EcoNI CCTNNNNNAGG 1 cut(s) 496
EcoO109I RGGNCCY 1 cut(s) 857
EcoO65I GGTNACC 1 cut(s) 552
EcoRII CCWGG 5 cut(s) 258, 491, 827, 859, 1123
EcoT14I CCWWGG 1 cut(s) 548
EcoT38I GRGCYC 1 cut(s) 860
EgeI GGCGCC 1 cut(s) 99
EheI GGCGCC 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 548
Esp3I CGTCTC 1 cut(s) 564
FaeI CATG 2 cut(s) 247, 853
FaiI YATR 7 cut(s) 245, 294, 512, 851, 915, 938, 1083
FaqI GGGAC 2 cut(s) 142, 931
FatI CATG 2 cut(s) 243, 849
FauI CCCGC 3 cut(s) 127, 512, 602
FblI GTMKAC 2 cut(s) 275, 1032
Fnu4HI GCNGC 9 cut(s) 85, 101, 140, 389, 507, 517, 631, 645, 839
FokI GGATG 3 cut(s) 109, 884, 983
FriOI GRGCYC 1 cut(s) 860
Fsp4HI GCNGC 9 cut(s) 85, 101, 140, 389, 507, 517, 631, 645, 839
FspBI CTAG 1 cut(s) 284
GlaI GCGC 2 cut(s) 99, 1072
GluI GCNGC 9 cut(s) 85, 101, 140, 389, 507, 517, 631, 645, 839
GsaI CCCAGC 3 cut(s) 8, 63, 193
GsuI CTGGAG 1 cut(s) 514
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 5 cut(s) 307, 633, 731, 820, 858
HapII CCGG 8 cut(s) 212, 299, 539, 753, 785, 928, 965, 1137
HhaI GCGC 2 cut(s) 100, 1073
Hin1I GRCGYC 2 cut(s) 98, 224
Hin1II CATG 2 cut(s) 247, 853
Hin6I GCGC 2 cut(s) 98, 1071
HinP1I GCGC 2 cut(s) 98, 1071
HincII GTYRAC 2 cut(s) 276, 1033
HindII GTYRAC 2 cut(s) 276, 1033
HinfI GANTC 4 cut(s) 106, 148, 461, 984
HpaII CCGG 8 cut(s) 212, 299, 539, 753, 785, 928, 965, 1137
HphI GGTGA 5 cut(s) 201, 241, 494, 546, 769
Hpy166II GTNNAC 3 cut(s) 276, 1033, 1143
Hpy188I TCNGA 4 cut(s) 113, 601, 901, 1009
Hpy8I GTNNAC 3 cut(s) 276, 1033, 1143
Hpy99I CGWCG 4 cut(s) 226, 229, 277, 280
HpyAV CCTTC 4 cut(s) 186, 340, 400, 787
HpyCH4III ACNGT 5 cut(s) 36, 73, 280, 558, 922
HpyCH4IV ACGT 2 cut(s) 224, 272
HpyCH4V TGCA 3 cut(s) 250, 908, 994
HpyF10VI GCNNNNNNNGC 4 cut(s) 356, 530, 539, 697
HpyF3I CTNAG 2 cut(s) 360, 842
HpySE526I ACGT 2 cut(s) 224, 272
Hsp92I GRCGYC 2 cut(s) 98, 224
Hsp92II CATG 2 cut(s) 247, 853
HspAI GCGC 2 cut(s) 98, 1071
KasI GGCGCC 1 cut(s) 97
KroI GCCGGC 1 cut(s) 538
KroNI GCCGGC 1 cut(s) 540
Kzo9I GATC 2 cut(s) 295, 711
LmnI GCTCC 4 cut(s) 139, 703, 952, 1120
Lsp1109I GCAGC 3 cut(s) 96, 656, 825
LweI GCATC 3 cut(s) 131, 215, 905
MaeI CTAG 1 cut(s) 284
MaeII ACGT 2 cut(s) 224, 272
MaeIII GTNAC 3 cut(s) 268, 552, 922
MalI GATC 2 cut(s) 297, 713
MbiI CCGCTC 1 cut(s) 516
MboI GATC 2 cut(s) 295, 711
MhlI GDGCHC 1 cut(s) 860
MlsI TGGCCA 1 cut(s) 731
MluCI AATT 6 cut(s) 181, 198, 216, 543, 958, 1131
MluNI TGGCCA 1 cut(s) 731
Mly113I GGCGCC 1 cut(s) 98
MlyI GAGTC 1 cut(s) 100
MmeI TCCRAC 2 cut(s) 579, 861
Mox20I TGGCCA 1 cut(s) 731
MroNI GCCGGC 1 cut(s) 538
MscI TGGCCA 1 cut(s) 731
MseI TTAA 1 cut(s) 813
MslI CAYNNNNRTG 3 cut(s) 71, 92, 901
Msp20I TGGCCA 1 cut(s) 731
MspI CCGG 8 cut(s) 212, 299, 539, 753, 785, 928, 965, 1137
Mva1269I GAATGC 1 cut(s) 772
MvaI CCWGG 5 cut(s) 260, 493, 829, 861, 1125
MvnI CGCG 1 cut(s) 1071
MwoI GCNNNNNNNGC 4 cut(s) 356, 530, 539, 697
NaeI GCCGGC 1 cut(s) 540
NarI GGCGCC 1 cut(s) 98
NciI CCSGG 6 cut(s) 754, 928, 929, 965, 1137, 1138
NdeII GATC 2 cut(s) 295, 711
NgoMIV GCCGGC 1 cut(s) 538
NlaIII CATG 2 cut(s) 247, 853
NlaIV GGNNCC 4 cut(s) 99, 858, 954, 1116
NmuCI GTSAC 3 cut(s) 268, 552, 922
NspV TTCGAA 1 cut(s) 179
OliI CACNNNNGTG 1 cut(s) 71
PcsI WCGNNNNNNNCGW 2 cut(s) 77, 1104
PctI GAATGC 1 cut(s) 772
PdiI GCCGGC 1 cut(s) 540
PfeI GAWTC 3 cut(s) 148, 461, 984
PflMI CCANNNNNTGG 1 cut(s) 75
PinAI ACCGGT 1 cut(s) 211
PkrI GCNGC 9 cut(s) 86, 102, 141, 390, 508, 518, 632, 646, 840
PleI GAGTC 1 cut(s) 100
PluTI GGCGCC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 100
Psp6I CCWGG 5 cut(s) 258, 491, 827, 859, 1123
PspEI GGTNACC 1 cut(s) 552
PspFI CCCAGC 3 cut(s) 4, 59, 189
PspGI CCWGG 5 cut(s) 258, 491, 827, 859, 1123
PspN4I GGNNCC 4 cut(s) 99, 858, 954, 1116
PspOMI GGGCCC 1 cut(s) 856
PspPI GGNCC 4 cut(s) 306, 818, 856, 857
RsaI GTAC 2 cut(s) 17, 326
RsaNI GTAC 2 cut(s) 16, 325
RseI CAYNNNNRTG 3 cut(s) 71, 92, 901
SalI GTCGAC 2 cut(s) 274, 1031
SaqAI TTAA 1 cut(s) 813
SatI GCNGC 9 cut(s) 85, 101, 140, 389, 507, 517, 631, 645, 839
Sau3AI GATC 2 cut(s) 295, 711
Sau96I GGNCC 4 cut(s) 306, 818, 856, 857
SchI GAGTC 1 cut(s) 100
SduI GDGCHC 1 cut(s) 860
SfaNI GCATC 3 cut(s) 131, 215, 905
SfoI GGCGCC 1 cut(s) 99
SfuI TTCGAA 1 cut(s) 179
SgrDI CGTCGACG 1 cut(s) 274
SmaI CCCGGG 2 cut(s) 929, 1138
SmiMI CAYNNNNRTG 3 cut(s) 71, 92, 901
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 6 cut(s) 181, 198, 216, 543, 958, 1131
SspDI GGCGCC 1 cut(s) 97
SspI AATATT 1 cut(s) 475
SspMI CTAG 1 cut(s) 284
StyI CCWWGG 1 cut(s) 548
TaaI ACNGT 5 cut(s) 36, 73, 280, 558, 922
TaiI ACGT 2 cut(s) 227, 275
TaqI TCGA 6 cut(s) 80, 179, 275, 378, 1032, 1045
TasI AATT 6 cut(s) 181, 198, 216, 543, 958, 1131
TatI WGTACW 1 cut(s) 324
TauI GCSGC 6 cut(s) 103, 142, 391, 509, 519, 633
TfiI GAWTC 3 cut(s) 148, 461, 984
Tru1I TTAA 1 cut(s) 813
Tru9I TTAA 1 cut(s) 813
TscAI CASTG 3 cut(s) 778, 927, 984
TseFI GTSAC 3 cut(s) 268, 552, 922
TseI GCWGC 3 cut(s) 84, 644, 838
Tsp45I GTSAC 3 cut(s) 268, 552, 922
TspGWI ACGGA 4 cut(s) 33, 115, 755, 965
TspMI CCCGGG 2 cut(s) 927, 1136
TspRI CASTG 3 cut(s) 778, 927, 984
Van91I CCANNNNNTGG 1 cut(s) 75
XagI CCTNNNNNAGG 1 cut(s) 496
XapI RAATTY 3 cut(s) 181, 958, 1131
XmaI CCCGGG 2 cut(s) 927, 1136
XmiI GTMKAC 2 cut(s) 275, 1032
XspI CTAG 1 cut(s) 284
ZraI GACGTC 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.