MD10G1253700.v1.1

DNA-damage-repair toleration protein DRT111

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014235
Physical Location & Seq
Reverse (-)
34620225 .. 34623765
3541 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1253700.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGCTAGGTGGTTTGTATGGAGACCTTCCTCCGCCCTCTTCCGCGGAGGAAGAGAAGCCCAGCAACAGCACGGTGTGGTCGAGCAGCACCAAAATGGCGCCGCAGACGCTCCGGAAGCCGTCGTCGATTTTTGCCCCGCCGCAGACGATTCTGAAGCCACAGAGCAAGCCTAAATCGTCGAATGCGGTGCAACCGAAAATTACAGCGTCACCGGCGGTTGCACTGTCGCCGGCGGTGATATACGATGAGGTGGAGCGACCTGAGCTGGTTGGGGTGACGTCGACGGTGCTGGAGGAGTATGATCCGGCGAGGCCGAATGACTATGAGGAGTACAGGAGGGAGCGGAAGAAGAAGGCAATGGAGGCCGAGATGAGGCGGGAACTAGATAGGAGGCGGCAGGAGGAGGAGGAAAGGGAGAGGAAAGAGAGGGAGGAGAGAGAAAGAGAGAAGGAGAGGGATTTGGGAGAATCAAGGAACATTTCAGGTGAGGAAGCCTGGAGGAGGCGTGCGGCGATGAGCGGGGCAGCAACGCCGGTAGTTCCGAGGTCCCCGTCCCCGCCCAGTAATGCAGATGGTTTTATCATTGGGAAGTCTGATAGTGGTGGGTTGGGGCTCGGGGCTGGCGGCCAAATGACCGCAGCACAAAGAATGATGGCGAAGATGGGGTGGAAGCAGGGTCAAGGGCTTGGTAAGCAGGAGCAGGGGATTACTACTCCCTTGATGGCCAAGAAAACGGATAGGCGAGCCGGGGTGATTGTGAATGCTAGTGAAACAAAGCCGGAGAAGAAGGCGAAGAGTGTCAGCCTTAACGGGCCACCCACGAGGGTTTTGCTGCTTAGAAACATGGTGGGCCCTGGAGAGGTGGATGATGAATTGGAAGATGAAGTAGCATCAGAGTGTGCAAAATATGGGACAGTGACGCGGGTGCTTATATTTGAGATAACAGAACCAAATTTCCCGGTGGATGAGGCGGTGAGAATCTTCATTCAGTTTGAGAGGTCTGAGGAAACGACTAAAGCATTGGTTGACCTTGACGGCCGGTACTTTGGAGGCAGAGTGGTGCGTGCCTCGTTCTATGATGAGGAGAGGTTCGGTAACAACGAGTTAGCTCCAATGCCAGGAGAAATCCCAGGTTTTACTTGA

Protein Analysis

381

Amino Acids

42.09

Weight (kDa)

5.53

Isoelectric Point (pI)

68.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-patch PF01585 212 - 252 4e-17 G-patch domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30480
fragaria_vesca FvH4_3g10030 FvH4_3g10030
malus_domestica MD05G1274000.v1.1 MD10G1253700.v1.1
prunus_persica Prupe.4G089800_v2.0.a1
pyrus_communis pycom05g25540 pycom10g20930
rosa_chinensis RchiOBHm_Chr5g0015891
rosa_laevigata RLG00000032229
rosa_multiflora Rmu_sc0001878.1_g000014
rosa_roxburghii Rroxscaffold_1G00060700
rosa_rugosa Rorug05G0030000
rosa_samantha Rh5AG123600 Rh5BG122500 Rh5CG133900
rosa_wichuraiana Rw5G010670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 281
AccB1I GGYRCC 1 cut(s) 97
AccBSI CCGCTC 2 cut(s) 343, 519
AccI GTMKAC 1 cut(s) 281
AccII CGCG 2 cut(s) 44, 922
AccIII TCCGGA 1 cut(s) 111
AclWI GGATC 1 cut(s) 296
AcoI YGGCCR 3 cut(s) 625, 723, 1036
AcsI RAATTY 1 cut(s) 952
AcuI CTGAAG 1 cut(s) 173
AcyI GRCGYC 2 cut(s) 98, 278
AdeI CACNNNGTG 1 cut(s) 75
AfaI GTAC 2 cut(s) 332, 1043
AfiI CCNNNNNNNGG 4 cut(s) 372, 501, 859, 1118
AjnI CCWGG 4 cut(s) 494, 853, 1117, 1129
AloI GAACNNNNNNTCC 2 cut(s) 939, 971
AluBI AGCT 2 cut(s) 265, 1109
AluI AGCT 2 cut(s) 265, 1109
Alw26I GTCTC 1 cut(s) 15
AlwI GGATC 1 cut(s) 296
Ama87I CYCGRG 1 cut(s) 614
Aor13HI TCCGGA 1 cut(s) 111
AoxI GGCC 7 cut(s) 311, 363, 625, 723, 812, 850, 1036
ApaI GGGCCC 1 cut(s) 854
ApeKI GCWGC 4 cut(s) 84, 524, 638, 832
ApoI RAATTY 1 cut(s) 952
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 4 cut(s) 546, 812, 850, 851
AsuC2I CCSGG 2 cut(s) 748, 959
AsuHPI GGTGA 6 cut(s) 201, 247, 286, 497, 763, 985
AvaI CYCGRG 1 cut(s) 614
AvaII GGWCC 1 cut(s) 546
BaeGI GKGCMC 1 cut(s) 854
BalI TGGCCA 1 cut(s) 725
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 2 cut(s) 615, 854
BauI CACGAG 1 cut(s) 820
BbvI GCAGC 4 cut(s) 96, 536, 650, 819
BccI CCATC 4 cut(s) 566, 646, 655, 715
BceAI ACGGC 2 cut(s) 103, 1051
BcgI CGANNNNNNTGC 4 cut(s) 169, 203, 811, 845
BciT130I CCWGG 4 cut(s) 496, 855, 1119, 1131
BcnI CCSGG 2 cut(s) 748, 959
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 3 cut(s) 5, 383, 765
BfoI RGCGCY 1 cut(s) 101
BisI GCNGC 9 cut(s) 85, 101, 140, 395, 510, 525, 625, 639, 833
BlsI GCNGC 9 cut(s) 86, 102, 141, 396, 511, 526, 626, 640, 834
Bme1390I CCNGG 6 cut(s) 496, 748, 855, 959, 1119, 1131
Bme18I GGWCC 1 cut(s) 546
BmeT110I CYCGRG 1 cut(s) 614
BmgT120I GGNCC 4 cut(s) 546, 812, 850, 851
BmiI GGNNCC 3 cut(s) 99, 548, 852
BmrFI CCNGG 6 cut(s) 496, 748, 855, 959, 1119, 1131
BmrI ACTGGG 1 cut(s) 555
BmsI GCATC 1 cut(s) 899
BmuI ACTGGG 1 cut(s) 555
BpmI CTGGAG 3 cut(s) 311, 517, 876
Bpu10I CCTNAGC 1 cut(s) 261
BpuMI CCSGG 2 cut(s) 748, 959
BsaHI GRCGYC 2 cut(s) 98, 278
BsaI GGTCTC 1 cut(s) 15
BsaJI CCNNGG 5 cut(s) 42, 542, 747, 853, 1129
BsaWI WCCGGW 1 cut(s) 111
BsaXI ACNNNNNCTCC 4 cut(s) 490, 520, 1041, 1071
Bsc4I CCNNNNNNNGG 4 cut(s) 372, 501, 859, 1118
Bse118I RCCGGY 4 cut(s) 211, 229, 532, 1038
Bse1I ACTGG 1 cut(s) 561
Bse3DI GCAATG 1 cut(s) 363
BseAI TCCGGA 1 cut(s) 111
BseBI CCWGG 4 cut(s) 496, 855, 1119, 1131
BseDI CCNNGG 5 cut(s) 42, 542, 747, 853, 1129
BseGI GGATG 2 cut(s) 871, 970
BseLI CCNNNNNNNGG 4 cut(s) 372, 501, 859, 1118
BseMI GCAATG 1 cut(s) 363
BseMII CTCAG 2 cut(s) 252, 993
BseNI ACTGG 1 cut(s) 561
BseRI GAGGAG 7 cut(s) 308, 341, 416, 419, 446, 514, 1097
BseSI GKGCMC 1 cut(s) 854
BseX3I CGGCCG 1 cut(s) 1036
BseXI GCAGC 4 cut(s) 96, 536, 650, 819
BseYI CCCAGC 1 cut(s) 59
Bsh1236I CGCG 2 cut(s) 44, 922
Bsh1285I CGRYCG 1 cut(s) 1039
BshFI GGCC 7 cut(s) 313, 365, 627, 725, 814, 852, 1038
BshNI GGYRCC 1 cut(s) 97
BsiEI CGRYCG 1 cut(s) 1039
BsiHKCI CYCGRG 1 cut(s) 614
BsiSI CCGG 9 cut(s) 112, 212, 230, 305, 533, 747, 779, 959, 1039
BslFI GGGAC 3 cut(s) 532, 538, 925
BslI CCNNNNNNNGG 4 cut(s) 372, 501, 859, 1118
BsmAI GTCTC 1 cut(s) 15
BsmFI GGGAC 3 cut(s) 532, 538, 925
BsmI GAATGC 2 cut(s) 187, 766
BsnI GGCC 7 cut(s) 313, 365, 627, 725, 814, 852, 1038
Bso31I GGTCTC 1 cut(s) 15
BsoBI CYCGRG 1 cut(s) 614
Bsp120I GGGCCC 1 cut(s) 850
Bsp1286I GDGCHC 2 cut(s) 615, 854
Bsp13I TCCGGA 1 cut(s) 111
Bsp143I GATC 1 cut(s) 301
BspANI GGCC 7 cut(s) 313, 365, 627, 725, 814, 852, 1038
BspCNI CTCAG 2 cut(s) 253, 994
BspEI TCCGGA 1 cut(s) 111
BspFNI CGCG 2 cut(s) 44, 922
BspLI GGNNCC 3 cut(s) 99, 548, 852
BspPI GGATC 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 97
BspTNI GGTCTC 1 cut(s) 15
BsrBI CCGCTC 2 cut(s) 343, 519
BsrDI GCAATG 1 cut(s) 363
BsrFI RCCGGY 4 cut(s) 211, 229, 532, 1038
BsrI ACTGG 1 cut(s) 561
BssAI RCCGGY 4 cut(s) 211, 229, 532, 1038
BssECI CCNNGG 5 cut(s) 42, 542, 747, 853, 1129
BssMI GATC 1 cut(s) 301
BssNI GRCGYC 2 cut(s) 98, 278
BssSI CACGAG 1 cut(s) 820
Bst2BI CACGAG 1 cut(s) 820
Bst2UI CCWGG 4 cut(s) 496, 855, 1119, 1131
Bst4CI ACNGT 4 cut(s) 73, 225, 286, 916
Bst6I CTCTTC 3 cut(s) 43, 45, 788
BstACI GRCGYC 2 cut(s) 98, 278
BstC8I GCNNGC 6 cut(s) 167, 231, 507, 622, 744, 1065
BstDEI CTNAG 3 cut(s) 261, 836, 1002
BstDSI CCRYGG 1 cut(s) 42
BstENI CCTNNNNNAGG 1 cut(s) 499
BstF5I GGATG 2 cut(s) 871, 970
BstFNI CGCG 2 cut(s) 44, 922
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 1 cut(s) 304
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 1 cut(s) 301
BstMCI CGRYCG 1 cut(s) 1039
BstMWI GCNNNNNNNGC 6 cut(s) 106, 115, 212, 262, 362, 691
BstNI CCWGG 4 cut(s) 496, 855, 1119, 1131
BstSCI CCNGG 6 cut(s) 494, 746, 853, 957, 1117, 1129
BstSLI GKGCMC 1 cut(s) 854
BstUI CGCG 2 cut(s) 44, 922
BstV1I GCAGC 4 cut(s) 96, 536, 650, 819
BstZI CGGCCG 1 cut(s) 1036
BsuRI GGCC 7 cut(s) 313, 365, 627, 725, 814, 852, 1038
BtgI CCRYGG 1 cut(s) 42
BtgZI GCGATG 1 cut(s) 527
BtsCI GGATG 2 cut(s) 871, 970
BtsIMutI CAGTG 2 cut(s) 221, 921
Cac8I GCNNGC 6 cut(s) 167, 231, 507, 622, 744, 1065
CfoI GCGC 1 cut(s) 100
Cfr10I RCCGGY 4 cut(s) 211, 229, 532, 1038
Cfr13I GGNCC 4 cut(s) 546, 812, 850, 851
Cfr42I CCGCGG 1 cut(s) 45
CseI GACGC 3 cut(s) 115, 195, 928
Csp6I GTAC 2 cut(s) 331, 1042
CviAII CATG 1 cut(s) 844
CviQI GTAC 2 cut(s) 331, 1042
DdeI CTNAG 3 cut(s) 261, 836, 1002
DinI GGCGCC 1 cut(s) 99
DpnI GATC 1 cut(s) 303
DpnII GATC 1 cut(s) 301
DraIII CACNNNGTG 1 cut(s) 75
EaeI YGGCCR 3 cut(s) 625, 723, 1036
EagI CGGCCG 1 cut(s) 1036
Eam1104I CTCTTC 3 cut(s) 43, 45, 788
EarI CTCTTC 3 cut(s) 43, 45, 788
EciI GGCGGA 1 cut(s) 21
EclXI CGGCCG 1 cut(s) 1036
Eco24I GRGCYC 2 cut(s) 615, 854
Eco31I GGTCTC 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 546
Eco52I CGGCCG 1 cut(s) 1036
Eco57I CTGAAG 1 cut(s) 173
Eco88I CYCGRG 1 cut(s) 614
EcoNI CCTNNNNNAGG 1 cut(s) 499
EcoO109I RGGNCCY 2 cut(s) 546, 851
EcoRII CCWGG 4 cut(s) 494, 853, 1117, 1129
EcoT38I GRGCYC 2 cut(s) 615, 854
EgeI GGCGCC 1 cut(s) 99
EheI GGCGCC 1 cut(s) 99
FaeI CATG 1 cut(s) 847
FaiI YATR 8 cut(s) 18, 241, 300, 324, 845, 909, 932, 1077
FaqI GGGAC 3 cut(s) 532, 538, 925
FatI CATG 1 cut(s) 843
FauI CCCGC 5 cut(s) 144, 369, 512, 564, 915
FblI GTMKAC 1 cut(s) 281
Fnu4HI GCNGC 9 cut(s) 85, 101, 140, 395, 510, 525, 625, 639, 833
FokI GGATG 2 cut(s) 878, 977
FriOI GRGCYC 2 cut(s) 615, 854
Fsp4HI GCNGC 9 cut(s) 85, 101, 140, 395, 510, 525, 625, 639, 833
FspBI CTAG 3 cut(s) 5, 383, 765
GlaI GCGC 1 cut(s) 99
GluI GCNGC 9 cut(s) 85, 101, 140, 395, 510, 525, 625, 639, 833
GsaI CCCAGC 1 cut(s) 63
GsuI CTGGAG 3 cut(s) 311, 517, 876
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 7 cut(s) 313, 365, 627, 725, 814, 852, 1038
HapII CCGG 9 cut(s) 112, 212, 230, 305, 533, 747, 779, 959, 1039
HgaI GACGC 3 cut(s) 115, 195, 928
HhaI GCGC 1 cut(s) 100
Hin1I GRCGYC 2 cut(s) 98, 278
Hin1II CATG 1 cut(s) 847
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HincII GTYRAC 2 cut(s) 282, 1027
HindII GTYRAC 2 cut(s) 282, 1027
HinfI GANTC 3 cut(s) 148, 467, 978
HpaII CCGG 9 cut(s) 112, 212, 230, 305, 533, 747, 779, 959, 1039
HphI GGTGA 6 cut(s) 201, 247, 286, 497, 763, 985
Hpy166II GTNNAC 2 cut(s) 282, 1027
Hpy188I TCNGA 5 cut(s) 153, 543, 595, 895, 1003
Hpy188III TCNNGA 1 cut(s) 112
Hpy8I GTNNAC 2 cut(s) 282, 1027
Hpy99I CGWCG 5 cut(s) 124, 127, 181, 283, 286
HpyAV CCTTC 4 cut(s) 35, 346, 442, 781
HpyCH4III ACNGT 4 cut(s) 73, 225, 286, 916
HpyCH4IV ACGT 1 cut(s) 278
HpyCH4V TGCA 4 cut(s) 190, 221, 569, 902
HpyF10VI GCNNNNNNNGC 6 cut(s) 106, 115, 212, 262, 362, 691
HpyF3I CTNAG 3 cut(s) 261, 836, 1002
HpySE526I ACGT 1 cut(s) 278
Hsp92I GRCGYC 2 cut(s) 98, 278
Hsp92II CATG 1 cut(s) 847
HspAI GCGC 1 cut(s) 98
KasI GGCGCC 1 cut(s) 97
Kpn2I TCCGGA 1 cut(s) 111
KroI GCCGGC 1 cut(s) 229
KroNI GCCGGC 1 cut(s) 231
KspI CCGCGG 1 cut(s) 45
Kzo9I GATC 1 cut(s) 301
LmnI GCTCC 5 cut(s) 114, 253, 340, 697, 1114
Lsp1109I GCAGC 4 cut(s) 96, 536, 650, 819
LweI GCATC 1 cut(s) 899
MaeI CTAG 3 cut(s) 5, 383, 765
MaeII ACGT 1 cut(s) 278
MaeIII GTNAC 4 cut(s) 207, 274, 916, 1094
MalI GATC 1 cut(s) 303
MbiI CCGCTC 2 cut(s) 343, 519
MboI GATC 1 cut(s) 301
MboII GAAGA 9 cut(s) 30, 62, 358, 361, 670, 796, 805, 890, 973
MhlI GDGCHC 2 cut(s) 615, 854
MlsI TGGCCA 1 cut(s) 725
MluCI AATT 3 cut(s) 198, 872, 952
MluNI TGGCCA 1 cut(s) 725
Mly113I GGCGCC 1 cut(s) 98
Mox20I TGGCCA 1 cut(s) 725
MreI CGCCGGCG 1 cut(s) 229
MroI TCCGGA 1 cut(s) 111
MroNI GCCGGC 1 cut(s) 229
MscI TGGCCA 1 cut(s) 725
MseI TTAA 1 cut(s) 807
MslI CAYNNNNRTG 2 cut(s) 92, 895
Msp20I TGGCCA 1 cut(s) 725
MspA1I CMGCKG 1 cut(s) 44
MspI CCGG 9 cut(s) 112, 212, 230, 305, 533, 747, 779, 959, 1039
MspR9I CCNGG 6 cut(s) 496, 748, 855, 959, 1119, 1131
Mva1269I GAATGC 2 cut(s) 187, 766
MvaI CCWGG 4 cut(s) 496, 855, 1119, 1131
MvnI CGCG 2 cut(s) 44, 922
MwoI GCNNNNNNNGC 6 cut(s) 106, 115, 212, 262, 362, 691
NaeI GCCGGC 1 cut(s) 231
NarI GGCGCC 1 cut(s) 98
NciI CCSGG 2 cut(s) 748, 959
NdeII GATC 1 cut(s) 301
NgoMIV GCCGGC 1 cut(s) 229
NlaIII CATG 1 cut(s) 847
NlaIV GGNNCC 3 cut(s) 99, 548, 852
NmeAIII GCCGAG 1 cut(s) 391
NmuCI GTSAC 3 cut(s) 207, 274, 916
PcsI WCGNNNNNNNCGW 2 cut(s) 77, 1098
PctI GAATGC 2 cut(s) 187, 766
PdiI GCCGGC 1 cut(s) 231
PfeI GAWTC 3 cut(s) 148, 467, 978
PkrI GCNGC 9 cut(s) 86, 102, 141, 396, 511, 526, 626, 640, 834
PluTI GGCGCC 1 cut(s) 101
PpuMI RGGWCCY 1 cut(s) 546
Psp5II RGGWCCY 1 cut(s) 546
Psp6I CCWGG 4 cut(s) 494, 853, 1117, 1129
PspFI CCCAGC 1 cut(s) 59
PspGI CCWGG 4 cut(s) 494, 853, 1117, 1129
PspN4I GGNNCC 3 cut(s) 99, 548, 852
PspOMI GGGCCC 1 cut(s) 850
PspPI GGNCC 4 cut(s) 546, 812, 850, 851
PspPPI RGGWCCY 1 cut(s) 546
RsaI GTAC 2 cut(s) 332, 1043
RsaNI GTAC 2 cut(s) 331, 1042
RseI CAYNNNNRTG 2 cut(s) 92, 895
SacII CCGCGG 1 cut(s) 45
SalI GTCGAC 1 cut(s) 280
SaqAI TTAA 1 cut(s) 807
SatI GCNGC 9 cut(s) 85, 101, 140, 395, 510, 525, 625, 639, 833
Sau3AI GATC 1 cut(s) 301
Sau96I GGNCC 4 cut(s) 546, 812, 850, 851
ScrFI CCNGG 6 cut(s) 496, 748, 855, 959, 1119, 1131
SduI GDGCHC 2 cut(s) 615, 854
SfaNI GCATC 1 cut(s) 899
SfoI GGCGCC 1 cut(s) 99
Sfr303I CCGCGG 1 cut(s) 45
SgrAI CRCCGGYG 2 cut(s) 211, 229
SgrBI CCGCGG 1 cut(s) 45
SgrDI CGTCGACG 1 cut(s) 280
SinI GGWCC 1 cut(s) 546
SmiMI CAYNNNNRTG 2 cut(s) 92, 895
Sse9I AATT 3 cut(s) 198, 872, 952
SspDI GGCGCC 1 cut(s) 97
SspMI CTAG 3 cut(s) 5, 383, 765
StyD4I CCNGG 6 cut(s) 494, 746, 853, 957, 1117, 1129
TaaI ACNGT 4 cut(s) 73, 225, 286, 916
TaiI ACGT 1 cut(s) 281
TaqI TCGA 4 cut(s) 80, 125, 179, 281
TasI AATT 3 cut(s) 198, 872, 952
TatI WGTACW 1 cut(s) 330
TauI GCSGC 5 cut(s) 103, 142, 397, 512, 627
TfiI GAWTC 3 cut(s) 148, 467, 978
Tru1I TTAA 1 cut(s) 807
Tru9I TTAA 1 cut(s) 807
TscAI CASTG 2 cut(s) 228, 921
TseFI GTSAC 3 cut(s) 207, 274, 916
TseI GCWGC 4 cut(s) 84, 524, 638, 832
Tsp45I GTSAC 3 cut(s) 207, 274, 916
TspDTI ATGAA 3 cut(s) 885, 897, 973
TspGWI ACGGA 1 cut(s) 749
TspRI CASTG 2 cut(s) 228, 921
VpaK11BI GGWCC 1 cut(s) 546
XagI CCTNNNNNAGG 1 cut(s) 499
XapI RAATTY 1 cut(s) 952
XmiI GTMKAC 1 cut(s) 281
XspI CTAG 3 cut(s) 5, 383, 765
ZraI GACGTC 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.