Rorug05G0030000

DNA-damage-repair toleration protein DRT111

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0014235
Physical Location & Seq
Forward (+)
1902563 .. 1905532
2970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0030000.1

Sequence Viewer

Length: 1443 bp
ATGGGCTCAATGAGTCTGACTTCACTTCTGGGTCATGGTTGCCGGAGTTTAGTTGCGCCGGTGGCCCTTGCGCGCACGTTGGCACTGACCCATGTTGCTCTGAGTGAACATTCGAAGAGCTACCAGCATGTGAAGGTGGCTTGGTTTTCTAAGAGGTCAAAGAAGAAGAAAAGGGTTGCATGTGAGTCTTTGAAAGAAGAGGTTACTTACCCAACTGCTGCTGCTGCTGCTTCTGTTTCTGTTTATGGCTACCCATATACTCGTTTACTTCCATGTCCCTCACAGAATGGACCACCAAGAGTTGAGCACTTGGTTGTTTCCGAAGGGGGCCCTGTTCTAGAATATATATGCAAAAATCTGGATCTTCCTGCTCAGTTTGTTGCAGATCTTATCCATTTTGGAGCTGTGTATTATGCCCTTGTATGTCCAAAGCCTCCTCCAACCGCGACGCCTGAGGAAATGAGGGTATATAAAGAAGCTACAGCACCATCAGTACTGAAAAAGAGAGCTTCTATAAAAGGAAAAACTGTACGAGAAGCACAGAAGACTTTCAGGATCACACATGTAGACCAATTTGTTGAAGATGGAACGTACTTACGTGTGCATGTACACCCAAAGCGTTTTCCTAGGTGCTATGAAATTGACTGGAAATCAAGAGTTATAGCAGTAAATGAAGACTTTGTGGTGTTGGACAAACCTGCTGGTACAACAGTTGGAGGAACCACAGACAACATCGAAGAAAGTTGTGCAACCTTTACCTCCCGTGCCTTAGGACTGACAACCCCCCTTATGACCACACATCAGATTGATAATTGTACAGAAGGATGTGTTGTCTTAGCTAGAACCAAGGAGTATTGTTCAGTTTTTCATGGAAAAATCCGAGAAAAACAAGTGAAGAAACTTTATCTTGCTCTTGCTGCTGCTCCTGTGCCACTTGGAACAATTACTCACTACATGCGTCCAATTAATGTGGCTCCACGACTCATTTCAGAAGATTCCATTGAAAGGTGGAGTTTGTGTCAGCTTGAGGTGATGGAATGCAAGGAAGTTCCCTGGCCAAATTCTGTCATCGAAGAGAAATACGGTGTTGAAGACTGTGGGTGGCCTTCAAAAGATTATGCATATGAGTGTAAAATCAACCTTTTGACTGGAAAGACTCATCAGATTCGAGCACAGTTGGCTGCTCATGGTGCACCTCTAGTGGGTGATTCCATGTACATGCCAGCTACAATTGCAGAGATTGCAAGTCCTGAGCTGAACCCGTTTGGCAAACATAAGAAACATTATATTGGCGAAGATGAGAAAGAAATAGCTGTTACTGAGTGGATGGCACGGCATGGAAAGGAACCTAATGTTGCTATTGGTCTTCAAGCTTGTCAGATATCATGGGATGATGGCGAGCACAGTTTTGAGGCTAGAGCTCCTTGGTGGCGGTGTGAATAA

Protein Analysis

480

Amino Acids

53.49

Weight (kDa)

8.22

Isoelectric Point (pI)

48.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 226 - 395 8e-27 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30480
fragaria_vesca FvH4_3g10030 FvH4_3g10030
malus_domestica MD05G1274000.v1.1 MD10G1253700.v1.1
prunus_persica Prupe.4G089800_v2.0.a1
pyrus_communis pycom05g25540 pycom10g20930
rosa_chinensis RchiOBHm_Chr5g0015891
rosa_laevigata RLG00000032229
rosa_multiflora Rmu_sc0001878.1_g000014
rosa_roxburghii Rroxscaffold_1G00060700
rosa_rugosa Rorug05G0030000
rosa_samantha Rh5AG123600 Rh5BG122500 Rh5CG133900
rosa_wichuraiana Rw5G010670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 706
AccI GTMKAC 1 cut(s) 567
AccII CGCG 2 cut(s) 73, 446
AciI CCGC 2 cut(s) 444, 1432
AclWI GGATC 2 cut(s) 369, 563
AcoI YGGCCR 1 cut(s) 1055
AcsI RAATTY 1 cut(s) 1060
AcyI GRCGYC 1 cut(s) 449
AfaI GTAC 7 cut(s) 495, 531, 593, 609, 706, 817, 1217
AfiI CCNNNNNNNGG 2 cut(s) 1005, 1202
AflIII ACRYGT 2 cut(s) 562, 598
AgsI TTSAA 6 cut(s) 193, 581, 1004, 1091, 1110, 1370
AjnI CCWGG 1 cut(s) 1052
Alw21I GWGCWC 5 cut(s) 309, 1174, 1195, 1404, 1423
Alw44I GTGCAC 1 cut(s) 1191
AlwI GGATC 2 cut(s) 369, 563
AoxI GGCC 4 cut(s) 63, 328, 1055, 1103
ApaI GGGCCC 1 cut(s) 332
ApaLI GTGCAC 1 cut(s) 1191
ApeKI GCWGC 7 cut(s) 218, 221, 224, 227, 917, 920, 1181
ApoI RAATTY 1 cut(s) 1060
AseI ATTAAT 1 cut(s) 966
Asp700I GAANNNNTTC 1 cut(s) 548
AspA2I CCTAGG 1 cut(s) 626
AspLEI GCGC 3 cut(s) 58, 73, 75
AspS9I GGNCC 4 cut(s) 64, 290, 328, 329
AsuHPI GGTGA 2 cut(s) 1042, 1217
AsuII TTCGAA 1 cut(s) 113
AvaII GGWCC 1 cut(s) 290
AvrII CCTAGG 1 cut(s) 626
AxyI CCTNAGG 2 cut(s) 453, 769
BaeGI GKGCMC 2 cut(s) 332, 1195
BaeI ACNNNNGTAYC 2 cut(s) 696, 729
BalI TGGCCA 1 cut(s) 1057
BanII GRGCYC 3 cut(s) 8, 332, 1423
BbsI GAAGAC 4 cut(s) 551, 681, 1098, 1358
Bbv12I GWGCWC 5 cut(s) 309, 1174, 1195, 1404, 1423
BbvI GCAGC 7 cut(s) 205, 208, 211, 214, 904, 907, 1168
BccI CCATC 5 cut(s) 496, 578, 1027, 1321, 1388
BceAI ACGGC 1 cut(s) 1349
BciT130I CCWGG 1 cut(s) 1054
BfaI CTAG 5 cut(s) 338, 627, 840, 1199, 1416
BfmI CTRYAG 1 cut(s) 480
BfuAI ACCTGC 1 cut(s) 706
BglII AGATCT 1 cut(s) 385
BisI GCNGC 7 cut(s) 219, 222, 225, 228, 918, 921, 1182
BlnI CCTAGG 1 cut(s) 626
BlsI GCNGC 7 cut(s) 220, 223, 226, 229, 919, 922, 1183
BmcAI AGTACT 1 cut(s) 495
Bme1390I CCNGG 1 cut(s) 1054
Bme18I GGWCC 1 cut(s) 290
BmgT120I GGNCC 4 cut(s) 64, 290, 328, 329
BmiI GGNNCC 5 cut(s) 329, 330, 721, 975, 1347
BmrFI CCNGG 1 cut(s) 1054
BpiI GAAGAC 4 cut(s) 551, 681, 1098, 1358
Bpu10I CCTNAGC 1 cut(s) 1251
Bpu14I TTCGAA 1 cut(s) 113
BpuEI CTTGAG 1 cut(s) 1046
BsaAI YACGTR 1 cut(s) 599
BsaHI GRCGYC 1 cut(s) 449
BsaJI CCNNGG 4 cut(s) 626, 846, 1052, 1424
BsaXI ACNNNNNCTCC 2 cut(s) 1003, 1033
Bsc4I CCNNNNNNNGG 2 cut(s) 1005, 1202
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 2 cut(s) 650, 1153
Bse21I CCTNAGG 2 cut(s) 453, 769
BseBI CCWGG 1 cut(s) 1054
BseDI CCNNGG 4 cut(s) 626, 846, 1052, 1424
BseGI GGATG 3 cut(s) 830, 1332, 1396
BseLI CCNNNNNNNGG 2 cut(s) 1005, 1202
BseMII CTCAG 5 cut(s) 92, 386, 444, 1242, 1311
BseNI ACTGG 2 cut(s) 650, 1153
BsePI GCGCGC 1 cut(s) 71
BseRI GAGGAG 1 cut(s) 426
BseSI GKGCMC 2 cut(s) 332, 1195
BseXI GCAGC 7 cut(s) 205, 208, 211, 214, 904, 907, 1168
Bsh1236I CGCG 2 cut(s) 73, 446
BshFI GGCC 4 cut(s) 65, 330, 1057, 1105
BsiHKAI GWGCWC 5 cut(s) 309, 1174, 1195, 1404, 1423
BsiSI CCGG 2 cut(s) 43, 59
BslFI GGGAC 1 cut(s) 261
BslI CCNNNNNNNGG 2 cut(s) 1005, 1202
BsmFI GGGAC 1 cut(s) 261
BsmI GAATGC 1 cut(s) 1043
BsnI GGCC 4 cut(s) 65, 330, 1057, 1105
Bsp119I TTCGAA 1 cut(s) 113
Bsp120I GGGCCC 1 cut(s) 328
Bsp1286I GDGCHC 7 cut(s) 8, 309, 332, 1174, 1195, 1404, 1423
Bsp1407I TGTACA 3 cut(s) 607, 815, 1215
Bsp143I GATC 3 cut(s) 361, 385, 555
BspACI CCGC 2 cut(s) 444, 1432
BspANI GGCC 4 cut(s) 65, 330, 1057, 1105
BspCNI CTCAG 5 cut(s) 93, 385, 445, 1243, 1312
BspFNI CGCG 2 cut(s) 73, 446
BspLI GGNNCC 5 cut(s) 329, 330, 721, 975, 1347
BspMI ACCTGC 1 cut(s) 706
BspPI GGATC 2 cut(s) 369, 563
BspQI GCTCTTC 1 cut(s) 110
BspT104I TTCGAA 1 cut(s) 113
BsrFI RCCGGY 1 cut(s) 58
BsrGI TGTACA 3 cut(s) 607, 815, 1215
BsrI ACTGG 2 cut(s) 650, 1153
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 4 cut(s) 626, 846, 1052, 1424
BssHII GCGCGC 1 cut(s) 71
BssMI GATC 3 cut(s) 361, 385, 555
BssNI GRCGYC 1 cut(s) 449
BssT1I CCWWGG 3 cut(s) 626, 846, 1424
Bst2UI CCWGG 1 cut(s) 1054
Bst4CI ACNGT 6 cut(s) 529, 712, 1085, 1097, 1176, 1406
Bst6I CTCTTC 3 cut(s) 110, 192, 1068
BstACI GRCGYC 1 cut(s) 449
BstAPI GCANNNNNTGC 1 cut(s) 1241
BstAUI TGTACA 3 cut(s) 607, 815, 1215
BstBAI YACGTR 1 cut(s) 599
BstBI TTCGAA 1 cut(s) 113
BstC8I GCNNGC 3 cut(s) 73, 1224, 1400
BstDEI CTNAG 8 cut(s) 101, 150, 372, 453, 769, 835, 1251, 1320
BstF5I GGATG 3 cut(s) 830, 1332, 1396
BstFNI CGCG 2 cut(s) 73, 446
BstHHI GCGC 3 cut(s) 58, 73, 75
BstKTI GATC 3 cut(s) 364, 388, 558
BstMBI GATC 3 cut(s) 361, 385, 555
BstMWI GCNNNNNNNGC 8 cut(s) 62, 224, 227, 917, 1178, 1190, 1232, 1241
BstNI CCWGG 1 cut(s) 1054
BstNSI RCATGY 6 cut(s) 131, 183, 566, 608, 958, 1222
BstSCI CCNGG 1 cut(s) 1052
BstSFI CTRYAG 1 cut(s) 480
BstSLI GKGCMC 2 cut(s) 332, 1195
BstUI CGCG 2 cut(s) 73, 446
BstV1I GCAGC 7 cut(s) 205, 208, 211, 214, 904, 907, 1168
BstV2I GAAGAC 4 cut(s) 551, 681, 1098, 1358
BstX2I RGATCY 2 cut(s) 361, 385
BstYI RGATCY 2 cut(s) 361, 385
Bsu36I CCTNAGG 2 cut(s) 453, 769
BsuRI GGCC 4 cut(s) 65, 330, 1057, 1105
BtsCI GGATG 3 cut(s) 830, 1332, 1396
BtsIMutI CAGTG 1 cut(s) 83
BveI ACCTGC 1 cut(s) 706
Cac8I GCNNGC 3 cut(s) 73, 1224, 1400
CfoI GCGC 3 cut(s) 58, 73, 75
Cfr10I RCCGGY 1 cut(s) 58
Cfr13I GGNCC 4 cut(s) 64, 290, 328, 329
CseI GACGC 2 cut(s) 457, 947
Csp6I GTAC 7 cut(s) 494, 530, 592, 608, 705, 816, 1216
CspCI CAANNNNNGTGG 2 cut(s) 951, 986
CviQI GTAC 7 cut(s) 494, 530, 592, 608, 705, 816, 1216
DdeI CTNAG 8 cut(s) 101, 150, 372, 453, 769, 835, 1251, 1320
DpnI GATC 3 cut(s) 363, 387, 557
DpnII GATC 3 cut(s) 361, 385, 555
EaeI YGGCCR 1 cut(s) 1055
Eam1104I CTCTTC 3 cut(s) 110, 192, 1068
EarI CTCTTC 3 cut(s) 110, 192, 1068
Ecl136II GAGCTC 1 cut(s) 1421
Eco130I CCWWGG 3 cut(s) 626, 846, 1424
Eco24I GRGCYC 3 cut(s) 8, 332, 1423
Eco32I GATATC 1 cut(s) 1383
Eco47I GGWCC 1 cut(s) 290
Eco53kI GAGCTC 1 cut(s) 1421
Eco81I CCTNAGG 2 cut(s) 453, 769
EcoICRI GAGCTC 1 cut(s) 1421
EcoO109I RGGNCCY 2 cut(s) 328, 329
EcoRII CCWGG 1 cut(s) 1052
EcoRV GATATC 1 cut(s) 1383
EcoT14I CCWWGG 3 cut(s) 626, 846, 1424
EcoT22I ATGCAT 1 cut(s) 1123
EcoT38I GRGCYC 3 cut(s) 8, 332, 1423
ErhI CCWWGG 3 cut(s) 626, 846, 1424
FaqI GGGAC 1 cut(s) 261
FauNDI CATATG 1 cut(s) 1123
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 7 cut(s) 219, 222, 225, 228, 918, 921, 1182
FokI GGATG 3 cut(s) 837, 1339, 1403
FriOI GRGCYC 3 cut(s) 8, 332, 1423
Fsp4HI GCNGC 7 cut(s) 219, 222, 225, 228, 918, 921, 1182
FspBI CTAG 5 cut(s) 338, 627, 840, 1199, 1416
GlaI GCGC 3 cut(s) 57, 72, 74
GluI GCNGC 7 cut(s) 219, 222, 225, 228, 918, 921, 1182
HaeIII GGCC 4 cut(s) 65, 330, 1057, 1105
HapII CCGG 2 cut(s) 43, 59
HgaI GACGC 2 cut(s) 457, 947
HhaI GCGC 3 cut(s) 58, 73, 75
Hin1I GRCGYC 1 cut(s) 449
Hin6I GCGC 3 cut(s) 56, 71, 73
HinP1I GCGC 3 cut(s) 56, 71, 73
HindIII AAGCTT 1 cut(s) 1371
HinfI GANTC 7 cut(s) 13, 185, 981, 995, 1156, 1165, 1208
HpaII CCGG 2 cut(s) 43, 59
HphI GGTGA 2 cut(s) 1042, 1217
Hpy166II GTNNAC 5 cut(s) 107, 266, 568, 610, 1193
Hpy188I TCNGA 8 cut(s) 18, 102, 322, 804, 881, 991, 1164, 1380
Hpy188III TCNNGA 5 cut(s) 338, 359, 553, 654, 1250
Hpy8I GTNNAC 5 cut(s) 107, 266, 568, 610, 1193
Hpy99I CGWCG 1 cut(s) 451
HpyAV CCTTC 4 cut(s) 127, 317, 815, 1116
HpyCH4III ACNGT 6 cut(s) 529, 712, 1085, 1097, 1176, 1406
HpyCH4IV ACGT 3 cut(s) 77, 590, 598
HpyF10VI GCNNNNNNNGC 8 cut(s) 62, 224, 227, 917, 1178, 1190, 1232, 1241
HpyF3I CTNAG 8 cut(s) 101, 150, 372, 453, 769, 835, 1251, 1320
HpySE526I ACGT 3 cut(s) 77, 590, 598
Hsp92I GRCGYC 1 cut(s) 449
HspAI GCGC 3 cut(s) 56, 71, 73
Kzo9I GATC 3 cut(s) 361, 385, 555
LguI GCTCTTC 1 cut(s) 110
LmnI GCTCC 4 cut(s) 401, 928, 979, 1426
Lsp1109I GCAGC 7 cut(s) 205, 208, 211, 214, 904, 907, 1168
MaeI CTAG 5 cut(s) 338, 627, 840, 1199, 1416
MaeII ACGT 3 cut(s) 77, 590, 598
MaeIII GTNAC 2 cut(s) 202, 1315
MalI GATC 3 cut(s) 363, 387, 557
MboI GATC 3 cut(s) 361, 385, 555
MfeI CAATTG 1 cut(s) 1230
MflI RGATCY 2 cut(s) 361, 385
MhlI GDGCHC 7 cut(s) 8, 309, 332, 1174, 1195, 1404, 1423
MlsI TGGCCA 1 cut(s) 1057
MluCI AATT 7 cut(s) 572, 639, 811, 942, 963, 1060, 1230
MluNI TGGCCA 1 cut(s) 1057
MlyI GAGTC 4 cut(s) 22, 194, 975, 1150
MmeI TCCRAC 3 cut(s) 464, 669, 694
Mox20I TGGCCA 1 cut(s) 1057
Mph1103I ATGCAT 1 cut(s) 1123
MroXI GAANNNNTTC 1 cut(s) 548
MscI TGGCCA 1 cut(s) 1057
MseI TTAA 1 cut(s) 966
MslI CAYNNNNRTG 2 cut(s) 1126, 1217
Msp20I TGGCCA 1 cut(s) 1057
MspI CCGG 2 cut(s) 43, 59
MspR9I CCNGG 1 cut(s) 1054
MunI CAATTG 1 cut(s) 1230
Mva1269I GAATGC 1 cut(s) 1043
MvaI CCWGG 1 cut(s) 1054
MvnI CGCG 2 cut(s) 73, 446
MwoI GCNNNNNNNGC 8 cut(s) 62, 224, 227, 917, 1178, 1190, 1232, 1241
NdeI CATATG 1 cut(s) 1123
NdeII GATC 3 cut(s) 361, 385, 555
NlaIV GGNNCC 5 cut(s) 329, 330, 721, 975, 1347
NsiI ATGCAT 1 cut(s) 1123
NspI RCATGY 6 cut(s) 131, 183, 566, 608, 958, 1222
NspV TTCGAA 1 cut(s) 113
PauI GCGCGC 1 cut(s) 71
PciI ACATGT 1 cut(s) 562
PciSI GCTCTTC 1 cut(s) 110
PctI GAATGC 1 cut(s) 1043
PdmI GAANNNNTTC 1 cut(s) 548
PfeI GAWTC 3 cut(s) 995, 1165, 1208
PkrI GCNGC 7 cut(s) 220, 223, 226, 229, 919, 922, 1183
PleI GAGTC 4 cut(s) 21, 193, 975, 1150
PpsI GAGTC 4 cut(s) 21, 193, 975, 1150
Ppu21I YACGTR 1 cut(s) 599
PscI ACATGT 1 cut(s) 562
PshBI ATTAAT 1 cut(s) 966
Psp124BI GAGCTC 1 cut(s) 1423
Psp6I CCWGG 1 cut(s) 1052
PspGI CCWGG 1 cut(s) 1052
PspN4I GGNNCC 5 cut(s) 329, 330, 721, 975, 1347
PspOMI GGGCCC 1 cut(s) 328
PspPI GGNCC 4 cut(s) 64, 290, 328, 329
PsuI RGATCY 2 cut(s) 361, 385
PteI GCGCGC 1 cut(s) 71
RsaI GTAC 7 cut(s) 495, 531, 593, 609, 706, 817, 1217
RsaNI GTAC 7 cut(s) 494, 530, 592, 608, 705, 816, 1216
RseI CAYNNNNRTG 2 cut(s) 1126, 1217
SacI GAGCTC 1 cut(s) 1423
SapI GCTCTTC 1 cut(s) 110
SaqAI TTAA 1 cut(s) 966
SatI GCNGC 7 cut(s) 219, 222, 225, 228, 918, 921, 1182
Sau3AI GATC 3 cut(s) 361, 385, 555
Sau96I GGNCC 4 cut(s) 64, 290, 328, 329
ScaI AGTACT 1 cut(s) 495
SchI GAGTC 4 cut(s) 22, 194, 975, 1150
ScrFI CCNGG 1 cut(s) 1054
SduI GDGCHC 7 cut(s) 8, 309, 332, 1174, 1195, 1404, 1423
SfcI CTRYAG 1 cut(s) 480
SfuI TTCGAA 1 cut(s) 113
SgrAI CRCCGGYG 1 cut(s) 58
SinI GGWCC 1 cut(s) 290
SmiMI CAYNNNNRTG 2 cut(s) 1126, 1217
SmlI CTYRAG 1 cut(s) 1025
SmoI CTYRAG 1 cut(s) 1025
Sse9I AATT 7 cut(s) 572, 639, 811, 942, 963, 1060, 1230
SsiI CCGC 2 cut(s) 444, 1432
SspMI CTAG 5 cut(s) 338, 627, 840, 1199, 1416
SstI GAGCTC 1 cut(s) 1423
StyD4I CCNGG 1 cut(s) 1052
StyI CCWWGG 3 cut(s) 626, 846, 1424
TaaI ACNGT 6 cut(s) 529, 712, 1085, 1097, 1176, 1406
TaiI ACGT 3 cut(s) 80, 593, 601
TaqI TCGA 4 cut(s) 113, 735, 1071, 1168
TasI AATT 7 cut(s) 572, 639, 811, 942, 963, 1060, 1230
TatI WGTACW 4 cut(s) 493, 607, 815, 1215
TfiI GAWTC 3 cut(s) 995, 1165, 1208
Tru1I TTAA 1 cut(s) 966
Tru9I TTAA 1 cut(s) 966
TscAI CASTG 1 cut(s) 90
TseI GCWGC 7 cut(s) 218, 221, 224, 227, 917, 920, 1181
TspDTI ATGAA 3 cut(s) 651, 687, 857
TspRI CASTG 1 cut(s) 90
VneI GTGCAC 1 cut(s) 1191
VpaK11BI GGWCC 1 cut(s) 290
VspI ATTAAT 1 cut(s) 966
XapI RAATTY 1 cut(s) 1060
XbaI TCTAGA 1 cut(s) 337
XceI RCATGY 6 cut(s) 131, 183, 566, 608, 958, 1222
XmaJI CCTAGG 1 cut(s) 626
XmiI GTMKAC 1 cut(s) 567
XmnI GAANNNNTTC 1 cut(s) 548
XspI CTAG 5 cut(s) 338, 627, 840, 1199, 1416
ZrmI AGTACT 1 cut(s) 495
Zsp2I ATGCAT 1 cut(s) 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.