FvH4_3g10030

DNA-damage-repair toleration protein DRT111

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014235
Physical Location & Seq
Forward (+)
5837216 .. 5840934
3719 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g10030.t2

Sequence Viewer

Length: 1161 bp
ATGCTAGGCGGGCTCTACGGCGACCTCCCTCCGCCGTCTTCGGCGGACGAGGACAACAAGCCCACCAACTCCACCGTCTGGTCCAGCACCACCAAAATGGCGCCGCAAACCCTCCGCAAGCCCTCTTCGATTTTCGCGGCACCGCAGACGATCCTCAAATCCCTGCCCAAGCCCAAGCCTACGGCGGCTAAAATCGCAGCGTCTCCGGTGGCCGCCGCGCCTGTGATTTACAGCGACGCGGTGCAGACGGCGTTGGTCGGCGTCACGTCGACGGTGCTGGAGGAGTACGATCCGGCCAGGCCGAACGACTACGAGGAGTACCGGAGGGAGAGGAAGAAGAAGGCGATGGAGGCCGAGGTGAGGAGGGAGCTCGATCGGCGGAGGATGGAGGAGGAGGAGAAGGAGAGGAGAGAGAGGGAGGAGAGAGAAGTTGAACGAGAAAGAGAAAGAGAGAGGGATAGAGATCGAGAAAGAGATGATTCAAGGCTGAACATTTCCGGCGAGGAAGCGTGGAGGAGAAGGGCGGCGATGAGTGGCTCTGCGGCGGTTCCGGTGGCTCCAAGGTCGCCGTCTCCGCCGAGCGATGCGGATGGTTTTATGATTGGGAAGTCGGAGAGCGGAGGACTGGGGGTGGGAGCTGGAGGGCAGATGACTGCGGCGCAGAGAATGATGGCGAAAATGGGGTGGAAGCAGGGGCAGGGGCTTGGGAAACTGGAGCAGGGGATTACTACTCCTTTGATGGCTAAGAAGACGGATAGGCGAGCCGGGGTGATTGTGAATGCAAGTGAAACAAAGCCGGAGAAGAAGGCCAAGGGTGTCAATCTCAATGGGCCGCCCACTCGGGTGCTGCTGCTCAGAAACATGGTGGGACCTGGTCAGGTGGATGACGAGTTAGAAGATGAAGTTGCATCAGAGTGTGCAAAGTATGGTACAGTGACCCGGGTGCTCATATTTGAGATAACAGAGCCAAATTTCCCGGTGGATGAAGCGGTAAGAATCTTTGTGCAGTTTGAGAGACCTGAAGAAACAACCAAAGCATTGGTTGACCTTGATGGTCGATACTTTGGAGGTAGAGTGGTCCGTGCCTCATTCTATGACGAGGAGCGGTTCGGTAACAATGAATTGGCTCCAATGCCAGGGGAAATCCCGGGTTTCACTTGA

Protein Analysis

387

Amino Acids

42.73

Weight (kDa)

5.65

Isoelectric Point (pI)

59.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-patch PF01585 218 - 258 2.3e-16 G-patch domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30480
fragaria_vesca FvH4_3g10030 FvH4_3g10030
malus_domestica MD05G1274000.v1.1 MD10G1253700.v1.1
prunus_persica Prupe.4G089800_v2.0.a1
pyrus_communis pycom05g25540 pycom10g20930
rosa_chinensis RchiOBHm_Chr5g0015891
rosa_laevigata RLG00000032229
rosa_multiflora Rmu_sc0001878.1_g000014
rosa_roxburghii Rroxscaffold_1G00060700
rosa_rugosa Rorug05G0030000
rosa_samantha Rh5AG123600 Rh5BG122500 Rh5CG133900
rosa_wichuraiana Rw5G010670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 100, 139
AccB7I CCANNNNNTGG 1 cut(s) 78
AccBSI CCGCTC 2 cut(s) 618, 1105
AccI GTMKAC 1 cut(s) 269
AccII CGCG 3 cut(s) 137, 218, 239
AclWI GGATC 2 cut(s) 145, 284
AcoI YGGCCR 2 cut(s) 210, 294
AcsI RAATTY 1 cut(s) 970
AcuI CTGAAG 1 cut(s) 1041
AcyI GRCGYC 2 cut(s) 101, 261
AfaI GTAC 3 cut(s) 287, 320, 931
AfiI CCNNNNNNNGG 3 cut(s) 78, 360, 1136
AgsI TTSAA 2 cut(s) 434, 483
AjiI CACGTC 1 cut(s) 267
AjnI CCWGG 3 cut(s) 296, 871, 1135
AleI CACNNNNGTG 1 cut(s) 842
AluBI AGCT 2 cut(s) 370, 638
AluI AGCT 2 cut(s) 370, 638
Alw21I GWGCWC 2 cut(s) 372, 948
Alw26I GTCTC 3 cut(s) 207, 576, 1009
AlwI GGATC 2 cut(s) 145, 284
Ama87I CYCGRG 3 cut(s) 840, 939, 1147
AoxI GGCC 6 cut(s) 210, 294, 299, 351, 807, 830
ApeKI GCWGC 3 cut(s) 197, 847, 850
ApoI RAATTY 1 cut(s) 970
AspLEI GCGC 3 cut(s) 103, 220, 661
AspS9I GGNCC 4 cut(s) 81, 830, 869, 1078
AsuC2I CCSGG 6 cut(s) 766, 940, 941, 977, 1148, 1149
AsuHPI GGTGA 2 cut(s) 370, 781
AvaI CYCGRG 3 cut(s) 840, 939, 1147
AvaII GGWCC 3 cut(s) 81, 869, 1078
BanI GGYRCC 2 cut(s) 100, 139
BanII GRGCYC 2 cut(s) 15, 372
BbsI GAAGAC 2 cut(s) 30, 755
Bbv12I GWGCWC 2 cut(s) 372, 948
BbvI GCAGC 3 cut(s) 209, 834, 837
BccI CCATC 6 cut(s) 340, 379, 584, 664, 733, 1046
BceAI ACGGC 5 cut(s) 19, 34, 198, 264, 553
BciT130I CCWGG 3 cut(s) 298, 873, 1137
BcnI CCSGG 6 cut(s) 766, 940, 941, 977, 1148, 1149
BcoDI GTCTC 3 cut(s) 207, 576, 1009
BfaI CTAG 1 cut(s) 5
BfoI RGCGCY 1 cut(s) 104
Bme1390I CCNGG 9 cut(s) 298, 766, 873, 940, 941, 977, 1137, 1148, 1149
Bme18I GGWCC 3 cut(s) 81, 869, 1078
BmeT110I CYCGRG 3 cut(s) 840, 939, 1147
BmgBI CACGTC 1 cut(s) 267
BmgT120I GGNCC 4 cut(s) 81, 830, 869, 1078
BmiI GGNNCC 6 cut(s) 102, 141, 549, 558, 870, 1128
BmrFI CCNGG 9 cut(s) 298, 766, 873, 940, 941, 977, 1137, 1148, 1149
BmrI ACTGGG 1 cut(s) 635
BmsI GCATC 2 cut(s) 574, 917
BmuI ACTGGG 1 cut(s) 635
BpiI GAAGAC 2 cut(s) 30, 755
BpmI CTGGAG 3 cut(s) 299, 660, 734
BpuMI CCSGG 6 cut(s) 766, 940, 941, 977, 1148, 1149
BsaBI GATNNNNATC 1 cut(s) 462
BsaHI GRCGYC 2 cut(s) 101, 261
BsaI GGTCTC 1 cut(s) 1009
BsaJI CCNNGG 7 cut(s) 354, 560, 765, 810, 939, 1136, 1147
BsaWI WCCGGW 3 cut(s) 205, 321, 550
BsaXI ACNNNNNCTCC 2 cut(s) 1059, 1089
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 360, 1136
Bse1I ACTGG 2 cut(s) 630, 717
Bse8I GATNNNNATC 1 cut(s) 462
BseBI CCWGG 3 cut(s) 298, 873, 1137
BseDI CCNNGG 7 cut(s) 354, 560, 765, 810, 939, 1136, 1147
BseGI GGATG 4 cut(s) 390, 595, 889, 988
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 3 cut(s) 78, 360, 1136
BseMII CTCAG 1 cut(s) 868
BseNI ACTGG 2 cut(s) 630, 717
BseXI GCAGC 3 cut(s) 209, 834, 837
BsgI GTGCAG 2 cut(s) 263, 1025
Bsh1236I CGCG 3 cut(s) 137, 218, 239
Bsh1285I CGRYCG 1 cut(s) 376
BshFI GGCC 6 cut(s) 212, 296, 301, 353, 809, 832
BshNI GGYRCC 2 cut(s) 100, 139
BsiEI CGRYCG 1 cut(s) 376
BsiHKAI GWGCWC 2 cut(s) 372, 948
BsiHKCI CYCGRG 3 cut(s) 840, 939, 1147
BslFI GGGAC 1 cut(s) 882
BslI CCNNNNNNNGG 3 cut(s) 78, 360, 1136
BsmAI GTCTC 3 cut(s) 207, 576, 1009
BsmBI CGTCTC 2 cut(s) 207, 576
BsmFI GGGAC 1 cut(s) 882
BsmI GAATGC 1 cut(s) 784
BsnI GGCC 6 cut(s) 212, 296, 301, 353, 809, 832
Bso31I GGTCTC 1 cut(s) 1009
BsoBI CYCGRG 3 cut(s) 840, 939, 1147
Bsp1286I GDGCHC 3 cut(s) 15, 372, 948
Bsp143I GATC 4 cut(s) 150, 289, 373, 463
BspANI GGCC 6 cut(s) 212, 296, 301, 353, 809, 832
BspCNI CTCAG 1 cut(s) 867
BspFNI CGCG 3 cut(s) 137, 218, 239
BspLI GGNNCC 6 cut(s) 102, 141, 549, 558, 870, 1128
BspPI GGATC 2 cut(s) 145, 284
BspT107I GGYRCC 2 cut(s) 100, 139
BspTNI GGTCTC 1 cut(s) 1009
BsrBI CCGCTC 2 cut(s) 618, 1105
BsrI ACTGG 2 cut(s) 630, 717
BssECI CCNNGG 7 cut(s) 354, 560, 765, 810, 939, 1136, 1147
BssMI GATC 4 cut(s) 150, 289, 373, 463
BssNI GRCGYC 2 cut(s) 101, 261
BssT1I CCWWGG 2 cut(s) 560, 810
Bst2UI CCWGG 3 cut(s) 298, 873, 1137
Bst4CI ACNGT 3 cut(s) 76, 274, 934
Bst6I CTCTTC 1 cut(s) 130
BstACI GRCGYC 2 cut(s) 101, 261
BstC8I GCNNGC 3 cut(s) 11, 119, 762
BstDEI CTNAG 2 cut(s) 744, 854
BstF5I GGATG 4 cut(s) 390, 595, 889, 988
BstFNI CGCG 3 cut(s) 137, 218, 239
BstH2I RGCGCY 1 cut(s) 104
BstHHI GCGC 3 cut(s) 103, 220, 661
BstKTI GATC 4 cut(s) 153, 292, 376, 466
BstMAI GTCTC 3 cut(s) 207, 576, 1009
BstMBI GATC 4 cut(s) 150, 289, 373, 463
BstMCI CGRYCG 1 cut(s) 376
BstMWI GCNNNNNNNGC 5 cut(s) 10, 194, 350, 376, 574
BstNI CCWGG 3 cut(s) 298, 873, 1137
BstSCI CCNGG 9 cut(s) 296, 764, 871, 938, 939, 975, 1135, 1146, 1147
BstUI CGCG 3 cut(s) 137, 218, 239
BstV1I GCAGC 3 cut(s) 209, 834, 837
BstV2I GAAGAC 2 cut(s) 30, 755
BstXI CCANNNNNNTGG 2 cut(s) 97, 1039
BsuRI GGCC 6 cut(s) 212, 296, 301, 353, 809, 832
BtgZI GCGATG 3 cut(s) 359, 542, 597
BtrI CACGTC 1 cut(s) 267
BtsCI GGATG 4 cut(s) 390, 595, 889, 988
BtsIMutI CAGTG 1 cut(s) 939
Cac8I GCNNGC 3 cut(s) 11, 119, 762
CfoI GCGC 3 cut(s) 103, 220, 661
Cfr13I GGNCC 4 cut(s) 81, 830, 869, 1078
Cfr9I CCCGGG 2 cut(s) 939, 1147
CseI GACGC 3 cut(s) 189, 245, 250
CsiI ACCWGGT 1 cut(s) 871
Csp6I GTAC 3 cut(s) 286, 319, 930
CviAII CATG 1 cut(s) 862
CviQI GTAC 3 cut(s) 286, 319, 930
DdeI CTNAG 2 cut(s) 744, 854
DinI GGCGCC 1 cut(s) 102
DpnI GATC 4 cut(s) 152, 291, 375, 465
DpnII GATC 4 cut(s) 150, 289, 373, 463
EaeI YGGCCR 2 cut(s) 210, 294
Eam1104I CTCTTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 130
EciI GGCGGA 4 cut(s) 21, 59, 394, 564
Ecl136II GAGCTC 1 cut(s) 370
Eco130I CCWWGG 2 cut(s) 560, 810
Eco24I GRGCYC 2 cut(s) 15, 372
Eco31I GGTCTC 1 cut(s) 1009
Eco47I GGWCC 3 cut(s) 81, 869, 1078
Eco53kI GAGCTC 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 1041
Eco88I CYCGRG 3 cut(s) 840, 939, 1147
EcoICRI GAGCTC 1 cut(s) 370
EcoO109I RGGNCCY 1 cut(s) 869
EcoRII CCWGG 3 cut(s) 296, 871, 1135
EcoT14I CCWWGG 2 cut(s) 560, 810
EcoT38I GRGCYC 2 cut(s) 15, 372
EgeI GGCGCC 1 cut(s) 102
EheI GGCGCC 1 cut(s) 102
ErhI CCWWGG 2 cut(s) 560, 810
Esp3I CGTCTC 2 cut(s) 207, 576
FaeI CATG 1 cut(s) 865
FaiI YATR 5 cut(s) 599, 863, 927, 950, 1095
FaqI GGGAC 1 cut(s) 882
FatI CATG 1 cut(s) 861
FauI CCCGC 1 cut(s) 2
FblI GTMKAC 1 cut(s) 269
FokI GGATG 4 cut(s) 397, 602, 896, 995
FriOI GRGCYC 2 cut(s) 15, 372
FspBI CTAG 1 cut(s) 5
GlaI GCGC 3 cut(s) 102, 219, 660
GsuI CTGGAG 3 cut(s) 299, 660, 734
HaeII RGCGCY 1 cut(s) 104
HaeIII GGCC 6 cut(s) 212, 296, 301, 353, 809, 832
HgaI GACGC 3 cut(s) 189, 245, 250
HhaI GCGC 3 cut(s) 103, 220, 661
Hin1I GRCGYC 2 cut(s) 101, 261
Hin1II CATG 1 cut(s) 865
Hin6I GCGC 3 cut(s) 101, 218, 659
HinP1I GCGC 3 cut(s) 101, 218, 659
HincII GTYRAC 2 cut(s) 270, 1045
HindII GTYRAC 2 cut(s) 270, 1045
HinfI GANTC 2 cut(s) 479, 996
HphI GGTGA 2 cut(s) 370, 781
Hpy166II GTNNAC 2 cut(s) 270, 1045
Hpy188I TCNGA 3 cut(s) 613, 857, 913
Hpy188III TCNNGA 1 cut(s) 467
Hpy8I GTNNAC 2 cut(s) 270, 1045
Hpy99I CGWCG 3 cut(s) 239, 271, 274
HpyAV CCTTC 4 cut(s) 334, 394, 513, 799
HpyCH4III ACNGT 3 cut(s) 76, 274, 934
HpyCH4IV ACGT 1 cut(s) 266
HpyCH4V TGCA 5 cut(s) 244, 782, 908, 920, 1006
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 194, 350, 376, 574
HpyF3I CTNAG 2 cut(s) 744, 854
HpySE526I ACGT 1 cut(s) 266
Hsp92I GRCGYC 2 cut(s) 101, 261
Hsp92II CATG 1 cut(s) 865
HspAI GCGC 3 cut(s) 101, 218, 659
KasI GGCGCC 1 cut(s) 100
Kzo9I GATC 4 cut(s) 150, 289, 373, 463
LmnI GCTCC 6 cut(s) 367, 562, 635, 715, 1102, 1132
Lsp1109I GCAGC 3 cut(s) 209, 834, 837
LweI GCATC 2 cut(s) 574, 917
MabI ACCWGGT 1 cut(s) 871
MaeI CTAG 1 cut(s) 5
MaeII ACGT 1 cut(s) 266
MaeIII GTNAC 3 cut(s) 262, 934, 1112
MalI GATC 4 cut(s) 152, 291, 375, 465
MbiI CCGCTC 2 cut(s) 618, 1105
MboI GATC 4 cut(s) 150, 289, 373, 463
MboII GAAGA 8 cut(s) 30, 117, 346, 349, 760, 814, 908, 1034
MhlI GDGCHC 3 cut(s) 15, 372, 948
MluCI AATT 2 cut(s) 970, 1121
Mly113I GGCGCC 1 cut(s) 101
MmeI TCCRAC 1 cut(s) 591
MslI CAYNNNNRTG 3 cut(s) 95, 842, 913
MspR9I CCNGG 9 cut(s) 298, 766, 873, 940, 941, 977, 1137, 1148, 1149
Mva1269I GAATGC 1 cut(s) 784
MvaI CCWGG 3 cut(s) 298, 873, 1137
MvnI CGCG 3 cut(s) 137, 218, 239
MwoI GCNNNNNNNGC 5 cut(s) 10, 194, 350, 376, 574
NarI GGCGCC 1 cut(s) 101
NciI CCSGG 6 cut(s) 766, 940, 941, 977, 1148, 1149
NdeII GATC 4 cut(s) 150, 289, 373, 463
NlaIII CATG 1 cut(s) 865
NlaIV GGNNCC 6 cut(s) 102, 141, 549, 558, 870, 1128
NmeAIII GCCGAG 2 cut(s) 379, 603
NmuCI GTSAC 2 cut(s) 262, 934
OliI CACNNNNGTG 1 cut(s) 842
PctI GAATGC 1 cut(s) 784
PfeI GAWTC 2 cut(s) 479, 996
PflFI GACNNNGTC 1 cut(s) 873
PflMI CCANNNNNTGG 1 cut(s) 78
Ple19I CGATCG 1 cut(s) 376
PluTI GGCGCC 1 cut(s) 104
PpuMI RGGWCCY 1 cut(s) 869
Psp124BI GAGCTC 1 cut(s) 372
Psp5II RGGWCCY 1 cut(s) 869
Psp6I CCWGG 3 cut(s) 296, 871, 1135
PspGI CCWGG 3 cut(s) 296, 871, 1135
PspN4I GGNNCC 6 cut(s) 102, 141, 549, 558, 870, 1128
PspPI GGNCC 4 cut(s) 81, 830, 869, 1078
PspPPI RGGWCCY 1 cut(s) 869
PsyI GACNNNGTC 1 cut(s) 873
PvuI CGATCG 1 cut(s) 376
RsaI GTAC 3 cut(s) 287, 320, 931
RsaNI GTAC 3 cut(s) 286, 319, 930
RseI CAYNNNNRTG 3 cut(s) 95, 842, 913
SacI GAGCTC 1 cut(s) 372
SalI GTCGAC 1 cut(s) 268
Sau3AI GATC 4 cut(s) 150, 289, 373, 463
Sau96I GGNCC 4 cut(s) 81, 830, 869, 1078
ScrFI CCNGG 9 cut(s) 298, 766, 873, 940, 941, 977, 1137, 1148, 1149
SduI GDGCHC 3 cut(s) 15, 372, 948
SexAI ACCWGGT 1 cut(s) 871
SfaNI GCATC 2 cut(s) 574, 917
SfoI GGCGCC 1 cut(s) 102
SgrDI CGTCGACG 1 cut(s) 268
SinI GGWCC 3 cut(s) 81, 869, 1078
SmaI CCCGGG 2 cut(s) 941, 1149
SmiMI CAYNNNNRTG 3 cut(s) 95, 842, 913
Sse9I AATT 2 cut(s) 970, 1121
SspDI GGCGCC 1 cut(s) 100
SspMI CTAG 1 cut(s) 5
SstI GAGCTC 1 cut(s) 372
StyD4I CCNGG 9 cut(s) 296, 764, 871, 938, 939, 975, 1135, 1146, 1147
StyI CCWWGG 2 cut(s) 560, 810
TaaI ACNGT 3 cut(s) 76, 274, 934
TaiI ACGT 1 cut(s) 269
TaqI TCGA 5 cut(s) 128, 269, 372, 466, 1057
TasI AATT 2 cut(s) 970, 1121
TauI GCSGC 9 cut(s) 106, 140, 188, 215, 218, 527, 545, 659, 835
TfiI GAWTC 2 cut(s) 479, 996
TscAI CASTG 1 cut(s) 939
TseFI GTSAC 2 cut(s) 262, 934
TseI GCWGC 3 cut(s) 197, 847, 850
Tsp45I GTSAC 2 cut(s) 262, 934
TspDTI ATGAA 3 cut(s) 915, 999, 1134
TspGWI ACGGA 2 cut(s) 767, 1070
TspMI CCCGGG 2 cut(s) 939, 1147
TspRI CASTG 1 cut(s) 939
Tth111I GACNNNGTC 1 cut(s) 873
Van91I CCANNNNNTGG 1 cut(s) 78
VpaK11BI GGWCC 3 cut(s) 81, 869, 1078
XapI RAATTY 1 cut(s) 970
XmaI CCCGGG 2 cut(s) 939, 1147
XmiI GTMKAC 1 cut(s) 269
XspI CTAG 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.