AT1G31720

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009203
Physical Location & Seq
Forward (+)
11356076 .. 11358095
2020 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G31720.7

Sequence Viewer

Length: 585 bp
ATGGAAATTCAAAAACAAGATAACCGGGATGGGCGACCCAAATCTTTTCTCTTTTTCATGTTCATCTTTCTCTTTGGTCTCGCGGCGTTCTTCCTATGCCTTTCGGCCGAGTTTCAAAAGGCTAAGGCTTTGCTAAGAGCTCAGGTTTTCTTAAAGGGGAAAGATCTGAAATGGGATGGAGAATCGTGTTATTTACCAGAAAATCGTGCTTTCGGGCTAGGAATTGCTGCTTTGGTCTGTGTTTCCGTTGCTCAGATCGTCGGAAACGTTGTGATCTGTAGAGGTTTCACGAAAACGGACAAAACCAGAACGACTATATTTTGTATAATTCTTCTGTTGTTTTCTTGGGTTAATTTTGCCGTTGCGGTTACGTTGATAAGCGTTGGTGCAAGCATGAATAGAGAGCAGATATACGGCAAAGGGTGGCTAAACCGTGAGTGTTACCTTGTGAAAGACGGTGTGTTCGCAGCGTCTGGCTTTCTCTCCGTTACGACGATGGCCGCTATTCTCGGAGCGTTTGCGTTTAAAGTCAAACCATCATTACAGGTCGAGAATCATGACAAACGTCACACTCAAAATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

194

Amino Acids

21.66

Weight (kDa)

9.43

Isoelectric Point (pI)

19.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 77 - 166 6.7e-20 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 83
AciI CCGC 3 cut(s) 83, 365, 501
AclI AACGTT 1 cut(s) 267
AcoI YGGCCR 2 cut(s) 105, 498
AcsI RAATTY 1 cut(s) 6
AgsI TTSAA 2 cut(s) 11, 116
AluBI AGCT 1 cut(s) 140
AluI AGCT 1 cut(s) 140
Alw21I GWGCWC 1 cut(s) 142
Alw26I GTCTC 1 cut(s) 83
AlwNI CAGNNNCTG 1 cut(s) 473
AoxI GGCC 2 cut(s) 105, 498
ApeKI GCWGC 2 cut(s) 227, 467
ApoI RAATTY 1 cut(s) 6
ArsI GACNNNNNNTTYG 2 cut(s) 446, 478
AsuC2I CCSGG 1 cut(s) 26
BanII GRGCYC 1 cut(s) 142
Bbv12I GWGCWC 1 cut(s) 142
BbvI GCAGC 2 cut(s) 214, 479
BccI CCATC 4 cut(s) 23, 170, 490, 544
BceAI ACGGC 2 cut(s) 344, 430
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 1 cut(s) 83
BfaI CTAG 1 cut(s) 218
BfmI CTRYAG 1 cut(s) 277
BglII AGATCT 1 cut(s) 163
BisI GCNGC 4 cut(s) 84, 228, 468, 501
BlsI GCNGC 4 cut(s) 85, 229, 469, 502
Bme1390I CCNGG 1 cut(s) 26
BmrFI CCNGG 1 cut(s) 26
BoxI GACNNNNGTC 1 cut(s) 564
Bpu10I CCTNAGC 2 cut(s) 123, 141
BpuMI CCSGG 1 cut(s) 26
BsaI GGTCTC 1 cut(s) 83
BseGI GGATG 2 cut(s) 34, 181
BseMII CTCAG 2 cut(s) 155, 266
BseX3I CGGCCG 1 cut(s) 105
BseXI GCAGC 2 cut(s) 214, 479
Bsh1236I CGCG 1 cut(s) 83
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 2 cut(s) 107, 500
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 142
BsiSI CCGG 1 cut(s) 25
BsmAI GTCTC 1 cut(s) 83
BsnI GGCC 2 cut(s) 107, 500
Bso31I GGTCTC 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 142
Bsp143I GATC 3 cut(s) 163, 255, 273
BspACI CCGC 3 cut(s) 83, 365, 501
BspANI GGCC 2 cut(s) 107, 500
BspCNI CTCAG 2 cut(s) 154, 265
BspFNI CGCG 1 cut(s) 83
BspHI TCATGA 1 cut(s) 556
BspTNI GGTCTC 1 cut(s) 83
BssMI GATC 3 cut(s) 163, 255, 273
Bst4CI ACNGT 2 cut(s) 434, 458
BstC8I GCNNGC 1 cut(s) 391
BstDEI CTNAG 4 cut(s) 123, 134, 141, 252
BstF5I GGATG 2 cut(s) 34, 181
BstFNI CGCG 1 cut(s) 83
BstKTI GATC 3 cut(s) 166, 258, 276
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 3 cut(s) 163, 255, 273
BstMCI CGRYCG 1 cut(s) 108
BstPAI GACNNNNGTC 1 cut(s) 564
BstSCI CCNGG 1 cut(s) 24
BstSFI CTRYAG 1 cut(s) 277
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 2 cut(s) 214, 479
BstX2I RGATCY 1 cut(s) 163
BstYI RGATCY 1 cut(s) 163
BstZI CGGCCG 1 cut(s) 105
BsuRI GGCC 2 cut(s) 107, 500
BtsCI GGATG 2 cut(s) 34, 181
Cac8I GCNNGC 1 cut(s) 391
CaiI CAGNNNCTG 1 cut(s) 473
CciI TCATGA 1 cut(s) 556
CseI GACGC 1 cut(s) 459
CviAII CATG 3 cut(s) 58, 394, 557
CviJI RGCY 8 cut(s) 107, 122, 128, 140, 217, 427, 477, 500
CviKI_1 RGCY 8 cut(s) 107, 122, 128, 140, 217, 427, 477, 500
DdeI CTNAG 4 cut(s) 123, 134, 141, 252
DpnI GATC 3 cut(s) 165, 257, 275
DpnII GATC 3 cut(s) 163, 255, 273
DraI TTTAAA 1 cut(s) 526
EaeI YGGCCR 2 cut(s) 105, 498
EagI CGGCCG 1 cut(s) 105
Ecl136II GAGCTC 1 cut(s) 140
EclXI CGGCCG 1 cut(s) 105
Eco24I GRGCYC 1 cut(s) 142
Eco31I GGTCTC 1 cut(s) 83
Eco52I CGGCCG 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 140
EcoT38I GRGCYC 1 cut(s) 142
FaeI CATG 3 cut(s) 61, 397, 560
FaiI YATR 7 cut(s) 59, 97, 317, 326, 395, 412, 558
FatI CATG 3 cut(s) 57, 393, 556
Fnu4HI GCNGC 4 cut(s) 84, 228, 468, 501
FokI GGATG 2 cut(s) 41, 188
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 4 cut(s) 84, 228, 468, 501
FspBI CTAG 1 cut(s) 218
GluI GCNGC 4 cut(s) 84, 228, 468, 501
HaeIII GGCC 2 cut(s) 107, 500
HapII CCGG 1 cut(s) 25
HgaI GACGC 1 cut(s) 459
Hin1II CATG 3 cut(s) 61, 397, 560
HinfI GANTC 2 cut(s) 182, 553
HpaII CCGG 1 cut(s) 25
Hpy188I TCNGA 4 cut(s) 168, 255, 263, 512
Hpy188III TCNNGA 3 cut(s) 289, 550, 557
Hpy99I CGWCG 2 cut(s) 263, 496
HpyCH4III ACNGT 2 cut(s) 434, 458
HpyCH4IV ACGT 3 cut(s) 267, 371, 565
HpyCH4V TGCA 1 cut(s) 389
HpyF3I CTNAG 4 cut(s) 123, 134, 141, 252
HpySE526I ACGT 3 cut(s) 267, 371, 565
Hsp92II CATG 3 cut(s) 61, 397, 560
Kzo9I GATC 3 cut(s) 163, 255, 273
LmnI GCTCC 1 cut(s) 512
LpnPI CCDG 6 cut(s) 38, 128, 210, 319, 459, 530
Lsp1109I GCAGC 2 cut(s) 214, 479
MaeI CTAG 1 cut(s) 218
MaeII ACGT 3 cut(s) 267, 371, 565
MaeIII GTNAC 4 cut(s) 367, 440, 487, 566
MalI GATC 3 cut(s) 165, 257, 275
MboI GATC 3 cut(s) 163, 255, 273
MboII GAAGA 2 cut(s) 82, 323
MflI RGATCY 1 cut(s) 163
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 4 cut(s) 6, 222, 327, 352
MmeI TCCRAC 1 cut(s) 241
MnlI CCTC 1 cut(s) 275
MseI TTAA 3 cut(s) 152, 351, 525
MspI CCGG 1 cut(s) 25
MspR9I CCNGG 1 cut(s) 26
MvnI CGCG 1 cut(s) 83
NciI CCSGG 1 cut(s) 26
NdeII GATC 3 cut(s) 163, 255, 273
NlaIII CATG 3 cut(s) 61, 397, 560
NmeAIII GCCGAG 1 cut(s) 133
NmuCI GTSAC 1 cut(s) 566
PagI TCATGA 1 cut(s) 556
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PfeI GAWTC 2 cut(s) 182, 553
PkrI GCNGC 4 cut(s) 85, 229, 469, 502
PshAI GACNNNNGTC 1 cut(s) 564
Psp124BI GAGCTC 1 cut(s) 142
Psp1406I AACGTT 1 cut(s) 267
PstNI CAGNNNCTG 1 cut(s) 473
PsuI RGATCY 1 cut(s) 163
SacI GAGCTC 1 cut(s) 142
SaqAI TTAA 3 cut(s) 152, 351, 525
SatI GCNGC 4 cut(s) 84, 228, 468, 501
Sau3AI GATC 3 cut(s) 163, 255, 273
ScrFI CCNGG 1 cut(s) 26
SduI GDGCHC 1 cut(s) 142
SetI ASST 8 cut(s) 142, 147, 270, 286, 374, 447, 549, 568
SfcI CTRYAG 1 cut(s) 277
Sse9I AATT 4 cut(s) 6, 222, 327, 352
SsiI CCGC 3 cut(s) 83, 365, 501
SspMI CTAG 1 cut(s) 218
SstI GAGCTC 1 cut(s) 142
StyD4I CCNGG 1 cut(s) 24
TaaI ACNGT 2 cut(s) 434, 458
TaiI ACGT 3 cut(s) 270, 374, 568
TaqI TCGA 1 cut(s) 549
TasI AATT 4 cut(s) 6, 222, 327, 352
TauI GCSGC 2 cut(s) 86, 503
TfiI GAWTC 2 cut(s) 182, 553
Tru1I TTAA 3 cut(s) 152, 351, 525
Tru9I TTAA 3 cut(s) 152, 351, 525
TseFI GTSAC 1 cut(s) 566
TseI GCWGC 2 cut(s) 227, 467
Tsp45I GTSAC 1 cut(s) 566
TspDTI ATGAA 3 cut(s) 46, 52, 410
TspGWI ACGGA 3 cut(s) 235, 311, 475
XapI RAATTY 1 cut(s) 6
XspI CTAG 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.