Rw7G024640

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009203
Physical Location & Seq
Forward (+)
30450736 .. 30451897
1162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G024640.1

Sequence Viewer

Length: 564 bp
ATGGAGAAACTCCAATACGGCTTGGTGCTAACTGTGTCTGTAGTCGCCTCCCTCGGCGTTGTCTCCTTCGTTTCATGCATAGCCGCTGAGATTAAGAGAACAAGGGAGGATGAGATCAAGTTGGTTGGAAGACTCTGTAAATTGCCTGGAAGTCACGCATTTGGGTTCGGAATTGCAGCATTGATCTGTTTAATTGTGGCTCAGGTGGTCGGAAATTTGGTAGTAATATGCAGTCATTTTTGTTCAAGAGAGAAGAAGAGTAGCGGTTCGAAAGCCAAACAACCAATGATTTTGATTGCTCTTCTGTTCATCTCTTGGATGAGCTTTGTAATGGCAGTGACATTGATAAGCACAGCTACTAGCATGAGCAGAAAGCAATCCTATGGAGAAGGATGGCTTGATGGTGAATGCTACCTGGTCAAACAAGGAATCTATATTGGTTCAGGAATACTGGTCCTGATTACTGTTGGTTCCACACTAGGCTTAGTGATCATAACTATGAGGAAGAGTCAAGTTGAAGAAGGCTTCAAAGGAGAGCAAAGCAAGCTGAACACCAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

187

Amino Acids

20.18

Weight (kDa)

9.3

Isoelectric Point (pI)

35.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 60 - 156 8.8e-21 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 84, 264
AcsI RAATTY 1 cut(s) 214
AgsI TTSAA 3 cut(s) 246, 518, 529
AjnI CCWGG 2 cut(s) 145, 414
AluBI AGCT 3 cut(s) 324, 356, 547
AluI AGCT 3 cut(s) 324, 356, 547
Alw26I GTCTC 1 cut(s) 67
ApeKI GCWGC 1 cut(s) 176
ApoI RAATTY 1 cut(s) 214
AspS9I GGNCC 1 cut(s) 454
AsuHPI GGTGA 1 cut(s) 416
AsuII TTCGAA 1 cut(s) 269
AvaII GGWCC 1 cut(s) 454
BbsI GAAGAC 1 cut(s) 136
BbvI GCAGC 1 cut(s) 188
BccI CCATC 2 cut(s) 387, 395
BceAI ACGGC 1 cut(s) 34
BciT130I CCWGG 2 cut(s) 147, 416
BclI TGATCA 1 cut(s) 489
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 2 cut(s) 360, 479
BfmI CTRYAG 1 cut(s) 39
BisI GCNGC 2 cut(s) 84, 177
BlsI GCNGC 2 cut(s) 85, 178
Bme1390I CCNGG 2 cut(s) 147, 416
Bme18I GGWCC 1 cut(s) 454
BmgT120I GGNCC 1 cut(s) 454
BmiI GGNNCC 1 cut(s) 472
BmrFI CCNGG 2 cut(s) 147, 416
BpiI GAAGAC 1 cut(s) 136
Bpu10I CCTNAGC 1 cut(s) 201
Bpu14I TTCGAA 1 cut(s) 269
BsaJI CCNNGG 1 cut(s) 52
Bse1I ACTGG 1 cut(s) 456
BseBI CCWGG 2 cut(s) 147, 416
BseDI CCNNGG 1 cut(s) 52
BseGI GGATG 3 cut(s) 115, 324, 398
BseMII CTCAG 2 cut(s) 78, 215
BseNI ACTGG 1 cut(s) 456
BseXI GCAGC 1 cut(s) 188
BsmAI GTCTC 1 cut(s) 67
BsmI GAATGC 1 cut(s) 413
Bsp119I TTCGAA 1 cut(s) 269
Bsp143I GATC 3 cut(s) 114, 183, 489
BspACI CCGC 2 cut(s) 84, 264
BspCNI CTCAG 2 cut(s) 79, 214
BspLI GGNNCC 1 cut(s) 472
BspQI GCTCTTC 1 cut(s) 306
BspT104I TTCGAA 1 cut(s) 269
BsrI ACTGG 1 cut(s) 456
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 3 cut(s) 114, 183, 489
Bst2UI CCWGG 2 cut(s) 147, 416
Bst4CI ACNGT 2 cut(s) 34, 466
Bst6I CTCTTC 3 cut(s) 251, 306, 500
BstBI TTCGAA 1 cut(s) 269
BstC8I GCNNGC 1 cut(s) 545
BstDEI CTNAG 3 cut(s) 87, 201, 484
BstF5I GGATG 3 cut(s) 115, 324, 398
BstKTI GATC 3 cut(s) 117, 186, 492
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 3 cut(s) 114, 183, 489
BstMWI GCNNNNNNNGC 1 cut(s) 544
BstNI CCWGG 2 cut(s) 147, 416
BstSCI CCNGG 2 cut(s) 145, 414
BstSFI CTRYAG 1 cut(s) 39
BstV1I GCAGC 1 cut(s) 188
BstV2I GAAGAC 1 cut(s) 136
BtsCI GGATG 3 cut(s) 115, 324, 398
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 1 cut(s) 545
Cfr13I GGNCC 1 cut(s) 454
CsiI ACCWGGT 1 cut(s) 414
CviAII CATG 2 cut(s) 75, 364
DdeI CTNAG 3 cut(s) 87, 201, 484
DpnI GATC 3 cut(s) 116, 185, 491
DpnII GATC 3 cut(s) 114, 183, 489
Eam1104I CTCTTC 3 cut(s) 251, 306, 500
EarI CTCTTC 3 cut(s) 251, 306, 500
Eco47I GGWCC 1 cut(s) 454
EcoRII CCWGG 2 cut(s) 145, 414
EcoT22I ATGCAT 1 cut(s) 80
FaeI CATG 2 cut(s) 78, 367
FaiI YATR 8 cut(s) 76, 80, 229, 365, 384, 435, 494, 500
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FatI CATG 2 cut(s) 74, 363
FbaI TGATCA 1 cut(s) 489
Fnu4HI GCNGC 2 cut(s) 84, 177
FokI GGATG 3 cut(s) 122, 331, 405
Fsp4HI GCNGC 2 cut(s) 84, 177
FspBI CTAG 2 cut(s) 360, 479
GluI GCNGC 2 cut(s) 84, 177
Hin1II CATG 2 cut(s) 78, 367
HinfI GANTC 3 cut(s) 132, 429, 508
HphI GGTGA 1 cut(s) 416
Hpy188I TCNGA 2 cut(s) 170, 212
Hpy188III TCNNGA 3 cut(s) 246, 444, 457
HpyAV CCTTC 3 cut(s) 76, 383, 515
HpyCH4III ACNGT 2 cut(s) 34, 466
HpyCH4V TGCA 3 cut(s) 78, 176, 231
HpyF10VI GCNNNNNNNGC 1 cut(s) 544
HpyF3I CTNAG 3 cut(s) 87, 201, 484
Hsp92II CATG 2 cut(s) 78, 367
Ksp22I TGATCA 1 cut(s) 489
Kzo9I GATC 3 cut(s) 114, 183, 489
LguI GCTCTTC 1 cut(s) 306
LpnPI CCDG 8 cut(s) 132, 159, 188, 401, 428, 429, 437, 470
Lsp1109I GCAGC 1 cut(s) 188
MabI ACCWGGT 1 cut(s) 414
MaeI CTAG 2 cut(s) 360, 479
MaeIII GTNAC 2 cut(s) 152, 337
MalI GATC 3 cut(s) 116, 185, 491
MboI GATC 3 cut(s) 114, 183, 489
MboII GAAGA 6 cut(s) 141, 265, 268, 293, 517, 530
MluCI AATT 4 cut(s) 140, 171, 192, 214
MlyI GAGTC 2 cut(s) 126, 517
MmeI TCCRAC 2 cut(s) 106, 190
MnlI CCTC 4 cut(s) 58, 62, 100, 495
Mph1103I ATGCAT 1 cut(s) 80
MseI TTAA 2 cut(s) 93, 191
MslI CAYNNNNRTG 1 cut(s) 497
MspA1I CMGCKG 1 cut(s) 86
MspR9I CCNGG 2 cut(s) 147, 416
Mva1269I GAATGC 1 cut(s) 413
MvaI CCWGG 2 cut(s) 147, 416
MwoI GCNNNNNNNGC 1 cut(s) 544
NdeII GATC 3 cut(s) 114, 183, 489
NlaIII CATG 2 cut(s) 78, 367
NlaIV GGNNCC 1 cut(s) 472
NmeAIII GCCGAG 1 cut(s) 33
NmuCI GTSAC 2 cut(s) 152, 337
NsiI ATGCAT 1 cut(s) 80
NspV TTCGAA 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 306
PctI GAATGC 1 cut(s) 413
PfeI GAWTC 1 cut(s) 429
PkrI GCNGC 2 cut(s) 85, 178
PleI GAGTC 2 cut(s) 126, 516
PpsI GAGTC 2 cut(s) 126, 516
Psp6I CCWGG 2 cut(s) 145, 414
PspGI CCWGG 2 cut(s) 145, 414
PspN4I GGNNCC 1 cut(s) 472
PspPI GGNCC 1 cut(s) 454
RseI CAYNNNNRTG 1 cut(s) 497
SapI GCTCTTC 1 cut(s) 306
SaqAI TTAA 2 cut(s) 93, 191
SatI GCNGC 2 cut(s) 84, 177
Sau3AI GATC 3 cut(s) 114, 183, 489
Sau96I GGNCC 1 cut(s) 454
SchI GAGTC 2 cut(s) 126, 517
ScrFI CCNGG 2 cut(s) 147, 416
SetI ASST 5 cut(s) 207, 326, 358, 417, 549
SexAI ACCWGGT 1 cut(s) 414
SfcI CTRYAG 1 cut(s) 39
SfuI TTCGAA 1 cut(s) 269
SinI GGWCC 1 cut(s) 454
SmiMI CAYNNNNRTG 1 cut(s) 497
Sse9I AATT 4 cut(s) 140, 171, 192, 214
SsiI CCGC 2 cut(s) 84, 264
SspMI CTAG 2 cut(s) 360, 479
StyD4I CCNGG 2 cut(s) 145, 414
TaaI ACNGT 2 cut(s) 34, 466
TaqI TCGA 1 cut(s) 269
TasI AATT 4 cut(s) 140, 171, 192, 214
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 1 cut(s) 429
Tru1I TTAA 2 cut(s) 93, 191
Tru9I TTAA 2 cut(s) 93, 191
TscAI CASTG 1 cut(s) 342
TseFI GTSAC 2 cut(s) 152, 337
TseI GCWGC 1 cut(s) 176
Tsp45I GTSAC 2 cut(s) 152, 337
TspDTI ATGAA 2 cut(s) 63, 298
TspRI CASTG 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 454
XapI RAATTY 1 cut(s) 214
XspI CTAG 2 cut(s) 360, 479
Zsp2I ATGCAT 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.