MD06G1011300.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009203
Physical Location & Seq
Reverse (-)
1343060 .. 1345538
2479 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1011300.v1.1.491

Sequence Viewer

Length: 603 bp
ATGGCGAAACATCAATATGAGATGGCGTTAACTCTTCTTGTAGTTGCCTCCCTTGGCATCGTCTCCTTCGTTTCATGCATAGCCGCCGAGATTAAGCGAACGAAGGACGACGAGCTCAAGTTGCTGGGAAGAATGTGTTATTTGCCAGAAAGTCAGGCATTTGGGTTCGGAGTTGCAGCCTTGATATGTTTGCTTGCTGCCCAGATCGTCGGAAATTTGATTTTTTGCAGTTATGTTTGTTCAAGAGATGAGAAGAAGAAGAAGATGAAGGGTACGTCTTCTACCACTACTACAACCACCAGCTCCAAAGCCAAAAGACCGATGGTTTCGATGGCTCTTTTGTCCATTTCTTGGATGAGCTTTGTAATAGGGGGGACATTGCTGAGCACGGCAACTAGCATGAGCAGAGAGCAACCGTACGGACGAGGATGGCTGGATGGGGAGTGCTACTTGGTTAGACAAGGAATCTATATTGGTTCAGGGATACTCATCCTCATTGCAATCGGTTGCACACTCGCCCTAATCATCGTCACCGCGACGAGGAGCCGAGTTGAAGATGGCTTTAAAGTAAAAGACCAAAGCACAAGTAGTGAATTGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

201

Amino Acids

21.64

Weight (kDa)

9.1

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 60 - 168 2.2e-18 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 351
AccII CGCG 1 cut(s) 536
AciI CCGC 2 cut(s) 84, 534
AcsI RAATTY 1 cut(s) 214
AfaI GTAC 2 cut(s) 274, 419
AfiI CCNNNNNNNGG 2 cut(s) 351, 540
AgsI TTSAA 2 cut(s) 243, 554
AluBI AGCT 3 cut(s) 115, 303, 360
AluI AGCT 3 cut(s) 115, 303, 360
Alw21I GWGCWC 2 cut(s) 117, 389
Alw26I GTCTC 1 cut(s) 67
ApeKI GCWGC 2 cut(s) 176, 197
ApoI RAATTY 1 cut(s) 214
AsuHPI GGTGA 1 cut(s) 523
BanII GRGCYC 1 cut(s) 117
BbsI GAAGAC 1 cut(s) 270
Bbv12I GWGCWC 2 cut(s) 117, 389
BbvI GCAGC 2 cut(s) 184, 188
BccI CCATC 6 cut(s) 16, 316, 325, 423, 431, 551
BceAI ACGGC 1 cut(s) 405
BciVI GTATCC 1 cut(s) 477
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 1 cut(s) 396
BfuI GTATCC 1 cut(s) 477
BisI GCNGC 3 cut(s) 84, 177, 198
BlpI GCTNAGC 1 cut(s) 383
BlsI GCNGC 3 cut(s) 85, 178, 199
BmiI GGNNCC 1 cut(s) 545
BmsI GCATC 1 cut(s) 66
BpiI GAAGAC 1 cut(s) 270
Bpu1102I GCTNAGC 1 cut(s) 383
BpuEI CTTGAG 1 cut(s) 101
BsaBI GATNNNNATC 1 cut(s) 488
BsaJI CCNNGG 1 cut(s) 52
Bsc4I CCNNNNNNNGG 2 cut(s) 351, 540
Bse3DI GCAATG 2 cut(s) 377, 495
Bse8I GATNNNNATC 1 cut(s) 488
BseDI CCNNGG 1 cut(s) 52
BseGI GGATG 4 cut(s) 360, 434, 442, 489
BseJI GATNNNNATC 1 cut(s) 488
BseLI CCNNNNNNNGG 2 cut(s) 351, 540
BseMI GCAATG 2 cut(s) 377, 495
BseMII CTCAG 1 cut(s) 374
BseRI GAGGAG 1 cut(s) 556
BseXI GCAGC 2 cut(s) 184, 188
BseYI CCCAGC 1 cut(s) 124
Bsh1236I CGCG 1 cut(s) 536
BsiHKAI GWGCWC 2 cut(s) 117, 389
BsiWI CGTACG 1 cut(s) 417
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 2 cut(s) 351, 540
BsmAI GTCTC 1 cut(s) 67
BsmBI CGTCTC 1 cut(s) 67
BsmFI GGGAC 1 cut(s) 388
Bsp1286I GDGCHC 2 cut(s) 117, 389
Bsp143I GATC 1 cut(s) 204
Bsp1720I GCTNAGC 1 cut(s) 383
BspACI CCGC 2 cut(s) 84, 534
BspCNI CTCAG 1 cut(s) 375
BspFNI CGCG 1 cut(s) 536
BspLI GGNNCC 1 cut(s) 545
BsrDI GCAATG 2 cut(s) 377, 495
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 52
Bst4CI ACNGT 1 cut(s) 417
Bst6I CTCTTC 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 195
BstDEI CTNAG 1 cut(s) 383
BstF5I GGATG 4 cut(s) 360, 434, 442, 489
BstFNI CGCG 1 cut(s) 536
BstKTI GATC 1 cut(s) 207
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 1 cut(s) 204
BstMWI GCNNNNNNNGC 1 cut(s) 121
BstUI CGCG 1 cut(s) 536
BstV1I GCAGC 2 cut(s) 184, 188
BstV2I GAAGAC 1 cut(s) 270
BsuI GTATCC 1 cut(s) 477
BtsCI GGATG 4 cut(s) 360, 434, 442, 489
Cac8I GCNNGC 1 cut(s) 195
Csp6I GTAC 2 cut(s) 273, 418
CviAII CATG 2 cut(s) 75, 400
CviQI GTAC 2 cut(s) 273, 418
DdeI CTNAG 1 cut(s) 383
DpnI GATC 1 cut(s) 206
DpnII GATC 1 cut(s) 204
DraI TTTAAA 1 cut(s) 565
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 1 cut(s) 52
Eco24I GRGCYC 1 cut(s) 117
Eco53kI GAGCTC 1 cut(s) 115
EcoICRI GAGCTC 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 52
EcoT22I ATGCAT 1 cut(s) 80
EcoT38I GRGCYC 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 52
Esp3I CGTCTC 1 cut(s) 67
FaeI CATG 2 cut(s) 78, 403
FaiI YATR 8 cut(s) 18, 76, 80, 187, 234, 401, 471, 601
FaqI GGGAC 1 cut(s) 388
FatI CATG 2 cut(s) 74, 399
Fnu4HI GCNGC 3 cut(s) 84, 177, 198
FokI GGATG 4 cut(s) 367, 441, 449, 476
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 3 cut(s) 84, 177, 198
FspBI CTAG 1 cut(s) 396
GluI GCNGC 3 cut(s) 84, 177, 198
GsaI CCCAGC 1 cut(s) 128
Hin1II CATG 2 cut(s) 78, 403
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 1 cut(s) 465
HpaI GTTAAC 1 cut(s) 30
HphI GGTGA 1 cut(s) 523
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 2 cut(s) 170, 212
Hpy188III TCNNGA 1 cut(s) 243
Hpy8I GTNNAC 1 cut(s) 30
Hpy99I CGWCG 3 cut(s) 113, 212, 541
HpyAV CCTTC 3 cut(s) 76, 97, 262
HpyCH4III ACNGT 1 cut(s) 417
HpyCH4IV ACGT 1 cut(s) 275
HpyCH4V TGCA 5 cut(s) 78, 176, 228, 500, 510
HpyF10VI GCNNNNNNNGC 1 cut(s) 121
HpyF3I CTNAG 1 cut(s) 383
HpySE526I ACGT 1 cut(s) 275
Hsp92II CATG 2 cut(s) 78, 403
KspAI GTTAAC 1 cut(s) 30
Kzo9I GATC 1 cut(s) 204
LmnI GCTCC 2 cut(s) 308, 543
LpnPI CCDG 7 cut(s) 110, 140, 159, 215, 313, 419, 465
Lsp1109I GCAGC 2 cut(s) 184, 188
LweI GCATC 1 cut(s) 66
MaeI CTAG 1 cut(s) 396
MaeII ACGT 1 cut(s) 275
MaeIII GTNAC 1 cut(s) 529
MalI GATC 1 cut(s) 206
MboI GATC 1 cut(s) 204
MboII GAAGA 8 cut(s) 26, 141, 265, 268, 270, 271, 274, 566
MhlI GDGCHC 2 cut(s) 117, 389
MluCI AATT 2 cut(s) 214, 593
MmeI TCCRAC 1 cut(s) 190
MnlI CCTC 4 cut(s) 58, 419, 503, 534
Mph1103I ATGCAT 1 cut(s) 80
MseI TTAA 3 cut(s) 29, 93, 564
MslI CAYNNNNRTG 1 cut(s) 15
MvnI CGCG 1 cut(s) 536
MwoI GCNNNNNNNGC 1 cut(s) 121
NdeII GATC 1 cut(s) 204
NlaIII CATG 2 cut(s) 78, 403
NlaIV GGNNCC 1 cut(s) 545
NmeAIII GCCGAG 2 cut(s) 112, 572
NmuCI GTSAC 1 cut(s) 529
NsiI ATGCAT 1 cut(s) 80
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PfeI GAWTC 1 cut(s) 465
Pfl23II CGTACG 1 cut(s) 417
PflMI CCANNNNNTGG 1 cut(s) 351
PkrI GCNGC 3 cut(s) 85, 178, 199
Psp124BI GAGCTC 1 cut(s) 117
PspFI CCCAGC 1 cut(s) 124
PspLI CGTACG 1 cut(s) 417
PspN4I GGNNCC 1 cut(s) 545
RsaI GTAC 2 cut(s) 274, 419
RsaNI GTAC 2 cut(s) 273, 418
RseI CAYNNNNRTG 1 cut(s) 15
SacI GAGCTC 1 cut(s) 117
SaqAI TTAA 3 cut(s) 29, 93, 564
SatI GCNGC 3 cut(s) 84, 177, 198
Sau3AI GATC 1 cut(s) 204
SduI GDGCHC 2 cut(s) 117, 389
SetI ASST 4 cut(s) 117, 278, 305, 362
SfaNI GCATC 1 cut(s) 66
SmiMI CAYNNNNRTG 1 cut(s) 15
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 2 cut(s) 214, 593
SsiI CCGC 2 cut(s) 84, 534
SspMI CTAG 1 cut(s) 396
SstI GAGCTC 1 cut(s) 117
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 1 cut(s) 417
TaiI ACGT 1 cut(s) 278
TaqI TCGA 1 cut(s) 329
TaqII GACCGA 1 cut(s) 334
TasI AATT 2 cut(s) 214, 593
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 1 cut(s) 465
Tru1I TTAA 3 cut(s) 29, 93, 564
Tru9I TTAA 3 cut(s) 29, 93, 564
TseFI GTSAC 1 cut(s) 529
TseI GCWGC 2 cut(s) 176, 197
Tsp45I GTSAC 1 cut(s) 529
TspDTI ATGAA 2 cut(s) 63, 281
TspGWI ACGGA 1 cut(s) 435
Van91I CCANNNNNTGG 1 cut(s) 351
XapI RAATTY 1 cut(s) 214
XcmI CCANNNNNNNNNTGG 1 cut(s) 319
XspI CTAG 1 cut(s) 396
Zsp2I ATGCAT 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.