AT4G19370

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009203
Physical Location & Seq
Reverse (-)
10566068 .. 10567838
1771 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G19370.1

Sequence Viewer

Length: 654 bp
ATGCACAACCTTTTTCTCTACTCAGTCGTGTTCTCTCTTGGTCTCGTTTCCTTCATCACATGTTTCGCAGCCGAGTTCAAGAGAACACAGAAAGAAGATATTCGATGGGACACAGAGAGGAACTGCTATGTACCCGGAAGCCATGCGTTCGGGCTTGGATCAGCCGCTGTTCTTTGCTTCTGCCTTGCTCAGATCGTTGGGAACATAGTTGTGTTCCGAAATCACCGGACAAGAACTAAAAGAGAAGATGGTTACAAGATTACTGATCTTACTTTACCTACGGTTCTATTGCTCCTGTCCTGGTCCAATTTTGTGGTAGTGGTATTGATTTTGAGTACCGCAATAAGCATGAGTCGAGCTCAAGCGTATGGAGAAGGATGGCTTGATGAAGATTGTTACTTGGTCAAAGACGGTGTCTTTGCAGCTTCAGGCTGCTTAGCCATCCTTGGACTTGGAGCTTTGACCATTTCAGCCACCAGGATCAAAGTAAAGAAACAACAACAACTTGTGCAAGTCGTAATCAAAGACCAAAACCAAGACCAAAGGAGATCAATGGAAGAAGAACAAAAGCATGATGAGCACCAAACGAACAAGTCAGAAAGTGTCATTCACTTGGTGGAAGAAGTGTCTTCCACTAATATAAGCAGAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

217

Amino Acids

24.39

Weight (kDa)

7.0

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 56 - 149 5.5e-15 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 165, 339
AclWI GGATC 2 cut(s) 166, 488
AcuI CTGAAG 1 cut(s) 411
AdeI CACNNNGTG 1 cut(s) 616
AfaI GTAC 2 cut(s) 132, 337
AflIII ACRYGT 1 cut(s) 59
AgsI TTSAA 1 cut(s) 79
AjnI CCWGG 2 cut(s) 299, 476
AluBI AGCT 3 cut(s) 359, 425, 458
AluI AGCT 3 cut(s) 359, 425, 458
Alw21I GWGCWC 2 cut(s) 361, 582
Alw26I GTCTC 1 cut(s) 47
AlwI GGATC 2 cut(s) 166, 488
AlwNI CAGNNNCTG 1 cut(s) 167
ApeKI GCWGC 3 cut(s) 68, 422, 432
ArsI GACNNNNNNTTYG 4 cut(s) 399, 401, 431, 433
Asp700I GAANNNNTTC 1 cut(s) 99
AspS9I GGNCC 1 cut(s) 303
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 1 cut(s) 215
AvaII GGWCC 1 cut(s) 303
BanII GRGCYC 1 cut(s) 361
BbsI GAAGAC 1 cut(s) 621
Bbv12I GWGCWC 2 cut(s) 361, 582
BbvI GCAGC 3 cut(s) 80, 419, 434
BccI CCATC 4 cut(s) 99, 242, 372, 449
BciT130I CCWGG 2 cut(s) 301, 478
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 47
BisI GCNGC 4 cut(s) 69, 165, 423, 433
BlpI GCTNAGC 1 cut(s) 436
BlsI GCNGC 4 cut(s) 70, 166, 424, 434
Bme1390I CCNGG 3 cut(s) 135, 301, 478
Bme18I GGWCC 1 cut(s) 303
BmgT120I GGNCC 1 cut(s) 303
BmrFI CCNGG 3 cut(s) 135, 301, 478
BpiI GAAGAC 1 cut(s) 621
BplI GAGNNNNNCTC 2 cut(s) 343, 375
Bpu1102I GCTNAGC 1 cut(s) 436
BpuEI CTTGAG 1 cut(s) 345
BpuMI CCSGG 1 cut(s) 135
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 1 cut(s) 445
BsaWI WCCGGW 1 cut(s) 225
BseBI CCWGG 2 cut(s) 301, 478
BseDI CCNNGG 1 cut(s) 445
BseGI GGATG 2 cut(s) 383, 441
BseMII CTCAG 2 cut(s) 36, 203
BseXI GCAGC 3 cut(s) 80, 419, 434
BsiHKAI GWGCWC 2 cut(s) 361, 582
BsiSI CCGG 2 cut(s) 135, 226
BslFI GGGAC 1 cut(s) 122
BsmAI GTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 122
Bso31I GGTCTC 1 cut(s) 47
Bsp1286I GDGCHC 2 cut(s) 361, 582
Bsp143I GATC 5 cut(s) 158, 192, 265, 480, 548
Bsp1720I GCTNAGC 1 cut(s) 436
BspACI CCGC 2 cut(s) 165, 339
BspCNI CTCAG 2 cut(s) 35, 202
BspPI GGATC 2 cut(s) 166, 488
BspTNI GGTCTC 1 cut(s) 47
BssECI CCNNGG 1 cut(s) 445
BssMI GATC 5 cut(s) 158, 192, 265, 480, 548
BssT1I CCWWGG 1 cut(s) 445
Bst2UI CCWGG 2 cut(s) 301, 478
Bst4CI ACNGT 2 cut(s) 283, 413
BstDEI CTNAG 3 cut(s) 22, 189, 436
BstF5I GGATG 2 cut(s) 383, 441
BstKTI GATC 5 cut(s) 161, 195, 268, 483, 551
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 5 cut(s) 158, 192, 265, 480, 548
BstMWI GCNNNNNNNGC 1 cut(s) 577
BstNI CCWGG 2 cut(s) 301, 478
BstNSI RCATGY 1 cut(s) 63
BstSCI CCNGG 3 cut(s) 133, 299, 476
BstV1I GCAGC 3 cut(s) 80, 419, 434
BstV2I GAAGAC 1 cut(s) 621
BstXI CCANNNNNNTGG 1 cut(s) 313
BtsCI GGATG 2 cut(s) 383, 441
CaiI CAGNNNCTG 1 cut(s) 167
Cfr13I GGNCC 1 cut(s) 303
Csp6I GTAC 2 cut(s) 131, 336
CviAII CATG 4 cut(s) 60, 143, 349, 572
CviQI GTAC 2 cut(s) 131, 336
DdeI CTNAG 3 cut(s) 22, 189, 436
DpnI GATC 5 cut(s) 160, 194, 267, 482, 550
DpnII GATC 5 cut(s) 158, 192, 265, 480, 548
DraIII CACNNNGTG 1 cut(s) 616
Ecl136II GAGCTC 1 cut(s) 359
Eco130I CCWWGG 1 cut(s) 445
Eco24I GRGCYC 1 cut(s) 361
Eco31I GGTCTC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 303
Eco53kI GAGCTC 1 cut(s) 359
Eco57I CTGAAG 1 cut(s) 411
EcoICRI GAGCTC 1 cut(s) 359
EcoRII CCWGG 2 cut(s) 299, 476
EcoT14I CCWWGG 1 cut(s) 445
EcoT38I GRGCYC 1 cut(s) 361
ErhI CCWWGG 1 cut(s) 445
FaeI CATG 4 cut(s) 63, 146, 352, 575
FaiI YATR 9 cut(s) 61, 129, 144, 206, 350, 369, 573, 641, 652
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FaqI GGGAC 1 cut(s) 122
FatI CATG 4 cut(s) 59, 142, 348, 571
Fnu4HI GCNGC 4 cut(s) 69, 165, 423, 433
FokI GGATG 2 cut(s) 390, 428
FriOI GRGCYC 1 cut(s) 361
Fsp4HI GCNGC 4 cut(s) 69, 165, 423, 433
GluI GCNGC 4 cut(s) 69, 165, 423, 433
HapII CCGG 2 cut(s) 135, 226
Hin1II CATG 4 cut(s) 63, 146, 352, 575
HinfI GANTC 1 cut(s) 352
HpaII CCGG 2 cut(s) 135, 226
HphI GGTGA 1 cut(s) 215
Hpy188I TCNGA 3 cut(s) 192, 218, 598
Hpy188III TCNNGA 1 cut(s) 79
HpyAV CCTTC 2 cut(s) 61, 368
HpyCH4III ACNGT 2 cut(s) 283, 413
HpyCH4V TGCA 3 cut(s) 4, 422, 511
HpyF10VI GCNNNNNNNGC 1 cut(s) 577
HpyF3I CTNAG 3 cut(s) 22, 189, 436
Hsp92II CATG 4 cut(s) 63, 146, 352, 575
Kzo9I GATC 5 cut(s) 158, 192, 265, 480, 548
LmnI GCTCC 2 cut(s) 297, 455
LpnPI CCDG 8 cut(s) 148, 239, 286, 308, 313, 414, 463, 490
Lsp1109I GCAGC 3 cut(s) 80, 419, 434
MaeIII GTNAC 2 cut(s) 251, 395
MalI GATC 5 cut(s) 160, 194, 267, 482, 550
MboI GATC 5 cut(s) 158, 192, 265, 480, 548
MboII GAAGA 7 cut(s) 107, 257, 401, 569, 572, 621, 632
MhlI GDGCHC 2 cut(s) 361, 582
MluCI AATT 1 cut(s) 307
MlyI GAGTC 1 cut(s) 361
MnlI CCTC 1 cut(s) 111
MroXI GAANNNNTTC 1 cut(s) 99
MslI CAYNNNNRTG 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 2 cut(s) 135, 226
MspR9I CCNGG 3 cut(s) 135, 301, 478
MvaI CCWGG 2 cut(s) 301, 478
MwoI GCNNNNNNNGC 1 cut(s) 577
NciI CCSGG 1 cut(s) 135
NdeII GATC 5 cut(s) 158, 192, 265, 480, 548
NlaIII CATG 4 cut(s) 63, 146, 352, 575
NmeAIII GCCGAG 1 cut(s) 97
NspI RCATGY 1 cut(s) 63
PciI ACATGT 1 cut(s) 59
PdmI GAANNNNTTC 1 cut(s) 99
PflFI GACNNNGTC 1 cut(s) 413
PkrI GCNGC 4 cut(s) 70, 166, 424, 434
PleI GAGTC 1 cut(s) 360
PpsI GAGTC 1 cut(s) 360
PscI ACATGT 1 cut(s) 59
Psp124BI GAGCTC 1 cut(s) 361
Psp6I CCWGG 2 cut(s) 299, 476
PspGI CCWGG 2 cut(s) 299, 476
PspPI GGNCC 1 cut(s) 303
PstNI CAGNNNCTG 1 cut(s) 167
PsyI GACNNNGTC 1 cut(s) 413
RsaI GTAC 2 cut(s) 132, 337
RsaNI GTAC 2 cut(s) 131, 336
RseI CAYNNNNRTG 1 cut(s) 209
SacI GAGCTC 1 cut(s) 361
SatI GCNGC 4 cut(s) 69, 165, 423, 433
Sau3AI GATC 5 cut(s) 158, 192, 265, 480, 548
Sau96I GGNCC 1 cut(s) 303
SchI GAGTC 1 cut(s) 361
ScrFI CCNGG 3 cut(s) 135, 301, 478
SduI GDGCHC 2 cut(s) 361, 582
SetI ASST 5 cut(s) 12, 280, 361, 427, 460
SinI GGWCC 1 cut(s) 303
SmiMI CAYNNNNRTG 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 360
SmoI CTYRAG 1 cut(s) 360
Sse9I AATT 1 cut(s) 307
SsiI CCGC 2 cut(s) 165, 339
SstI GAGCTC 1 cut(s) 361
StyD4I CCNGG 3 cut(s) 133, 299, 476
StyI CCWWGG 1 cut(s) 445
TaaI ACNGT 2 cut(s) 283, 413
TaqI TCGA 2 cut(s) 103, 355
TasI AATT 1 cut(s) 307
TauI GCSGC 1 cut(s) 167
TseI GCWGC 3 cut(s) 68, 422, 432
TspDTI ATGAA 2 cut(s) 43, 402
Tth111I GACNNNGTC 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 303
XceI RCATGY 1 cut(s) 63
XmnI GAANNNNTTC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.