FvH4_5g24980

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009203
Physical Location & Seq
Forward (+)
16486700 .. 16488145
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g24980.t1

Sequence Viewer

Length: 567 bp
ATGGAGAAACACCAATACGGCTTGGTGCTAACTGTTTCTGTAGTCGCCTCCCTCGGCGTTGTCTCCTTCGTTTCATGTATAGCTGCAGAGACTAAGAGAATTAGGGAGGATGAGGTCAAGTTGGTCGGAAGACTCTGTAAATTGCCTGAAAGTCATGCATTTGTGTTCGGAATTGCAGCTTTGATATGCTTGGTTGTTGCTCAGGTGATCGGGAATTTGGCAGCTATACGCATTCATTTTTGTTCAACAGAGAAGAAGAGTAGCGGTTCCAAAGCCAAACAGCCAGCGATTTTGATTGCTCTTCTGTTCATCTCTTGGGTGAGCTTTGTGATAGCAGTGACATTGATAAGCACAGCTACTAGCATGAGCAGAAAGCAGTCCTATGGAGAAGGATGGTTGGATGGTGAATGCTACCTTGTCAAGCAAGGAATCTATATTGGTTCAGGAATACTGGTCCTGGTTACTGTTGGTTCCACACTAGGCTTAGCGATCACAACTATGAGGAAGAGTCAAGTTGAAGAAGGCTTCATAGGAGGAGAGCAAAGAAAGTTGAACACCAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

189

Amino Acids

20.22

Weight (kDa)

9.28

Isoelectric Point (pI)

31.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 60 - 156 2.1e-20 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 264
AcsI RAATTY 1 cut(s) 214
AgsI TTSAA 3 cut(s) 246, 518, 553
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 5 cut(s) 83, 179, 224, 324, 356
AluI AGCT 5 cut(s) 83, 179, 224, 324, 356
Alw26I GTCTC 2 cut(s) 67, 83
ApeKI GCWGC 3 cut(s) 83, 176, 221
ApoI RAATTY 1 cut(s) 214
AspS9I GGNCC 1 cut(s) 454
AsuHPI GGTGA 3 cut(s) 217, 331, 416
AvaII GGWCC 1 cut(s) 454
BbsI GAAGAC 1 cut(s) 136
BbvI GCAGC 3 cut(s) 70, 188, 233
BccI CCATC 2 cut(s) 387, 395
BceAI ACGGC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 458
BcoDI GTCTC 2 cut(s) 67, 83
BfaI CTAG 2 cut(s) 360, 479
BfmI CTRYAG 2 cut(s) 39, 84
BisI GCNGC 3 cut(s) 84, 177, 222
BlpI GCTNAGC 1 cut(s) 484
BlsI GCNGC 3 cut(s) 85, 178, 223
Bme1390I CCNGG 1 cut(s) 458
Bme18I GGWCC 1 cut(s) 454
BmgT120I GGNCC 1 cut(s) 454
BmiI GGNNCC 2 cut(s) 268, 472
BmrFI CCNGG 1 cut(s) 458
BpiI GAAGAC 1 cut(s) 136
Bpu10I CCTNAGC 1 cut(s) 201
Bpu1102I GCTNAGC 1 cut(s) 484
BsaJI CCNNGG 1 cut(s) 52
Bse1I ACTGG 1 cut(s) 456
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 1 cut(s) 52
BseGI GGATG 3 cut(s) 115, 398, 406
BseMII CTCAG 1 cut(s) 215
BseNI ACTGG 1 cut(s) 456
BseRI GAGGAG 1 cut(s) 549
BseXI GCAGC 3 cut(s) 70, 188, 233
BsmAI GTCTC 2 cut(s) 67, 83
BsmI GAATGC 2 cut(s) 231, 413
Bsp143I GATC 2 cut(s) 207, 489
Bsp1720I GCTNAGC 1 cut(s) 484
BspACI CCGC 1 cut(s) 264
BspCNI CTCAG 1 cut(s) 214
BspLI GGNNCC 2 cut(s) 268, 472
BspMAI CTGCAG 1 cut(s) 88
BspQI GCTCTTC 1 cut(s) 306
BsrI ACTGG 1 cut(s) 456
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 2 cut(s) 207, 489
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 2 cut(s) 34, 466
Bst6I CTCTTC 3 cut(s) 251, 306, 500
BstC8I GCNNGC 1 cut(s) 285
BstDEI CTNAG 3 cut(s) 93, 201, 484
BstF5I GGATG 3 cut(s) 115, 398, 406
BstKTI GATC 2 cut(s) 210, 492
BstMAI GTCTC 2 cut(s) 67, 83
BstMBI GATC 2 cut(s) 207, 489
BstNI CCWGG 1 cut(s) 458
BstSCI CCNGG 1 cut(s) 456
BstSFI CTRYAG 2 cut(s) 39, 84
BstV1I GCAGC 3 cut(s) 70, 188, 233
BstV2I GAAGAC 1 cut(s) 136
BtsCI GGATG 3 cut(s) 115, 398, 406
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 1 cut(s) 285
Cfr13I GGNCC 1 cut(s) 454
CviAII CATG 3 cut(s) 75, 155, 364
DdeI CTNAG 3 cut(s) 93, 201, 484
DpnI GATC 2 cut(s) 209, 491
DpnII GATC 2 cut(s) 207, 489
Eam1104I CTCTTC 3 cut(s) 251, 306, 500
EarI CTCTTC 3 cut(s) 251, 306, 500
Eco47I GGWCC 1 cut(s) 454
EcoRII CCWGG 1 cut(s) 456
EcoT22I ATGCAT 1 cut(s) 160
FaeI CATG 3 cut(s) 78, 158, 367
FatI CATG 3 cut(s) 74, 154, 363
Fnu4HI GCNGC 3 cut(s) 84, 177, 222
FokI GGATG 3 cut(s) 122, 405, 413
Fsp4HI GCNGC 3 cut(s) 84, 177, 222
FspBI CTAG 2 cut(s) 360, 479
GluI GCNGC 3 cut(s) 84, 177, 222
Hin1II CATG 3 cut(s) 78, 158, 367
HinfI GANTC 3 cut(s) 132, 429, 508
HphI GGTGA 3 cut(s) 217, 331, 416
Hpy188I TCNGA 2 cut(s) 128, 170
Hpy188III TCNNGA 2 cut(s) 211, 444
HpyAV CCTTC 3 cut(s) 76, 383, 515
HpyCH4III ACNGT 2 cut(s) 34, 466
HpyCH4V TGCA 3 cut(s) 86, 158, 176
HpyF3I CTNAG 3 cut(s) 93, 201, 484
Hsp92II CATG 3 cut(s) 78, 158, 367
Kzo9I GATC 2 cut(s) 207, 489
LguI GCTCTTC 1 cut(s) 306
LpnPI CCDG 7 cut(s) 159, 188, 297, 429, 437, 443, 470
Lsp1109I GCAGC 3 cut(s) 70, 188, 233
MaeI CTAG 2 cut(s) 360, 479
MaeIII GTNAC 2 cut(s) 337, 460
MalI GATC 2 cut(s) 209, 491
MboI GATC 2 cut(s) 207, 489
MboII GAAGA 6 cut(s) 141, 265, 268, 293, 517, 530
MluCI AATT 4 cut(s) 99, 140, 171, 214
MlyI GAGTC 2 cut(s) 126, 517
MmeI TCCRAC 2 cut(s) 106, 378
MnlI CCTC 6 cut(s) 58, 62, 100, 106, 495, 527
Mph1103I ATGCAT 1 cut(s) 160
MslI CAYNNNNRTG 1 cut(s) 497
MspR9I CCNGG 1 cut(s) 458
Mva1269I GAATGC 2 cut(s) 231, 413
MvaI CCWGG 1 cut(s) 458
NdeII GATC 2 cut(s) 207, 489
NlaIII CATG 3 cut(s) 78, 158, 367
NlaIV GGNNCC 2 cut(s) 268, 472
NmeAIII GCCGAG 1 cut(s) 33
NmuCI GTSAC 1 cut(s) 337
NsiI ATGCAT 1 cut(s) 160
PciSI GCTCTTC 1 cut(s) 306
PctI GAATGC 2 cut(s) 231, 413
PfeI GAWTC 1 cut(s) 429
PkrI GCNGC 3 cut(s) 85, 178, 223
PleI GAGTC 2 cut(s) 126, 516
PpsI GAGTC 2 cut(s) 126, 516
Psp6I CCWGG 1 cut(s) 456
PspGI CCWGG 1 cut(s) 456
PspN4I GGNNCC 2 cut(s) 268, 472
PspPI GGNCC 1 cut(s) 454
PstI CTGCAG 1 cut(s) 88
RseI CAYNNNNRTG 1 cut(s) 497
SapI GCTCTTC 1 cut(s) 306
SatI GCNGC 3 cut(s) 84, 177, 222
Sau3AI GATC 2 cut(s) 207, 489
Sau96I GGNCC 1 cut(s) 454
SchI GAGTC 2 cut(s) 126, 517
ScrFI CCNGG 1 cut(s) 458
SetI ASST 8 cut(s) 85, 117, 181, 207, 226, 326, 358, 417
SfcI CTRYAG 2 cut(s) 39, 84
SinI GGWCC 1 cut(s) 454
SmiMI CAYNNNNRTG 1 cut(s) 497
Sse9I AATT 4 cut(s) 99, 140, 171, 214
SsiI CCGC 1 cut(s) 264
SspMI CTAG 2 cut(s) 360, 479
StyD4I CCNGG 1 cut(s) 456
TaaI ACNGT 2 cut(s) 34, 466
TasI AATT 4 cut(s) 99, 140, 171, 214
TfiI GAWTC 1 cut(s) 429
TscAI CASTG 1 cut(s) 342
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 3 cut(s) 83, 176, 221
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 4 cut(s) 63, 224, 298, 517
TspRI CASTG 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 454
XapI RAATTY 1 cut(s) 214
XspI CTAG 2 cut(s) 360, 479
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.