AT1G33250

Protein of unknown function, DUF604

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011882
Physical Location & Seq
Reverse (-)
12057198 .. 12059792
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G33250.1

Sequence Viewer

Length: 1647 bp
ATGCGTTTAATTCTCACCGTCATGTGCTCATTCATTCCTTACCTTTATTCATCTTCTCCTCACCGTCCTTGCTCTTCCCCAATCTCTCGTCCTCCTCCTCGAATCCGTCCTTGTCGTCTCAGTCGTTTCGCCACCGCATTAGTCGCTACCTCCGCTCTTCTTCTCGCTTCCGTCGCTTGGCTTTCTCTCGTCTTCTCACCAACCACTTCCCGTTGTTGGCATCTTCTTAAAGATTGGGAAGACAATCATCTCTGGAACAAACGGTACCATCATCCAATTGTAACTCCTCCTCCGCCGCCGCCGTCGCCGCCGTCTCTTCCAGCTCTTCCGTTATTCGATCATGAATTTAGAAATCGTAGTCTCTCAGAGATTGATAAACTTGATCTGTCTATGAATCACTTGATGTTTGGTATTGCTGGATCTTCACAGCTTTGGGAACGTCGTAAGGAACTTGTGAGATTATGGTGGAAACCTTCTCAGATGCGTGGACATGTCTGGCTTGAAGAGCAAGTTTCTCCTGAGGAAGGTGATGATTCTCTTCCTCCTATAATTGTCTCTGAAGACAGCTCTCGTTTTCGTTACACTAATCCTACTGGTCATCCTTCTGGACTTCGAATCTCTCGTATTGCTATGGAGTCTTTTCGTCTCTCTCTCCCTAATGTTCGGTGGTTTGTTCTTGGTGATGATGATACTATCTTCAATGTTCATAATCTTCTTGCTGTGTTAAGCAAGTATGATCCTTCGGAGATGGTTTATATTGGAAACCCCTCGGAGAGCCACTCTGCTAACTCTTACTTTAGTCATAACATGGCTTTTGGAGGCGGTGGCATTGCCATTAGCTACCCTTTAGCGGAAGCTCTCTCCCGGATTCACGACGATTGTCTCGATAGATACCCGAAGCTATACGGTAGTGATGATCGTCTCCACGCCTGCATCACTGAACTCGGAGTTCCTTTGTCCAGAGAGCCTGGCTTTCACCAGTGGGATATTAAGGGAAATGCTCATGGTCTCTTGTCCTCTCATCCTATTGCACCATTTGTCTCAATTCATCACGTTGAAGCTGTTAATCCTCTCTATCCGGGATTATCTACATTAGATAGTTTGAAACTCTTGACAAGAGCTATGAGTTTGGATCCTAGGAGTGTTTTGCAGCGATCGATCTGCTATGATCATACTCACAAACTGACATTTGCGATATCTCTTGGTTATGTTGTACAAGTTTTCCCAAGTATTCTCCTACCACGGGATCTTGAGCGAGCTGAACTTTCTTTCTCTGCTTGGAACGGGATAAGTCAACCCAGTGAGTTTGATCTTGACATAAAGCTTCCAATTTCGTCTCTCTGTAAGAAGCCTATCCTCTTCTTCTTGAAAGAGGTTGGCCAAGAAGGTAATGCTACACTTGGAACGTACTCAAGATCCTTGGTAAAGGATGATTTGAAGACGAAGCTTCTATGTTTTCCACGCTCTCTACCTCTGCACAAAGTGGATAAAATCCAGGTCTCAGGGTTTCCTCTGAGTAAAAATTGGCACTTGGCGCCGAGACGGTTGTGTTGCAGAGCAACCCCAACTACTACTAACGAGCCTCTCAGATTAACAGTTGGGCAGTGTGGTAAGATAATTTTGGGTTCCACGATTAGTTCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

548

Amino Acids

61.67

Weight (kDa)

8.74

Isoelectric Point (pI)

52.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fringe PF02434 219 - 327 1.2e-16 Fringe-like
DUF604 PF04646 263 - 518 2e-97 Protein of unknown function, DUF604
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33250
fragaria_vesca FvH4_5g10130
malus_domestica MD06G1127900.v1.1 MD14G1143700.v1.1
prunus_persica Prupe.5G137700_v2.0.a1 Prupe.5G137700_v2.0.a1
pyrus_communis pycom06g11820 pycom14g11910
rosa_chinensis RchiOBHm_Chr7g0188171
rosa_laevigata RLG00000004722
rosa_multiflora Rmu_sc0004001.1_g000001
rosa_roxburghii Rroxscaffold_3G00266710
rosa_rugosa Rorug06G0488800 Rorug06G0488900.1
rosa_samantha Rh7AG093000 Rh7BG094600 Rh7CG094300 Rh7DG095000
rosa_wichuraiana Rw7G008030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 264
AccB1I GGYRCC 2 cut(s) 264, 1534
AccBSI CCGCTC 1 cut(s) 155
AciI CCGC 8 cut(s) 135, 153, 293, 296, 299, 308, 822, 851
AclWI GGATC 6 cut(s) 427, 731, 1127, 1140, 1254, 1410
AcoI YGGCCR 1 cut(s) 1378
AcsI RAATTY 1 cut(s) 344
AcuI CTGAAG 1 cut(s) 579
AcyI GRCGYC 1 cut(s) 1535
AdeI CACNNNGTG 1 cut(s) 1483
AfaI GTAC 3 cut(s) 266, 1215, 1409
AfiI CCNNNNNNNGG 5 cut(s) 177, 216, 524, 850, 1243
AflIII ACRYGT 1 cut(s) 490
AgsI TTSAA 6 cut(s) 503, 700, 1058, 1105, 1369, 1438
AjnI CCWGG 2 cut(s) 967, 1494
Alw21I GWGCWC 1 cut(s) 29
AlwI GGATC 6 cut(s) 427, 731, 1127, 1140, 1254, 1410
AoxI GGCC 1 cut(s) 1378
ApeKI GCWGC 1 cut(s) 1150
ApoI RAATTY 1 cut(s) 344
Asp718I GGTACC 1 cut(s) 264
AspA2I CCTAGG 1 cut(s) 1136
AspLEI GCGC 1 cut(s) 1537
AsuC2I CCSGG 2 cut(s) 865, 1080
AsuHPI GGTGA 6 cut(s) 7, 53, 189, 539, 692, 968
AsuII TTCGAA 1 cut(s) 613
AvrII CCTAGG 1 cut(s) 1136
AxyI CCTNAGG 1 cut(s) 519
BalI TGGCCA 1 cut(s) 1380
BamHI GGATCC 1 cut(s) 1132
BanI GGYRCC 2 cut(s) 264, 1534
BbsI GAAGAC 4 cut(s) 184, 246, 567, 1445
Bbv12I GWGCWC 1 cut(s) 29
BbvI GCAGC 1 cut(s) 1162
BccI CCATC 2 cut(s) 276, 742
BceAI ACGGC 2 cut(s) 286, 295
BcgI CGANNNNNNTGC 2 cut(s) 1143, 1177
BciT130I CCWGG 2 cut(s) 969, 1496
BclI TGATCA 1 cut(s) 1168
BcnI CCSGG 2 cut(s) 865, 1080
BfaI CTAG 1 cut(s) 1137
BfoI RGCGCY 1 cut(s) 1538
BisI GCNGC 4 cut(s) 296, 299, 308, 1151
BlnI CCTAGG 1 cut(s) 1136
BlsI GCNGC 4 cut(s) 297, 300, 309, 1152
Bme1390I CCNGG 4 cut(s) 865, 969, 1080, 1496
BmiI GGNNCC 4 cut(s) 266, 1134, 1536, 1627
BmrFI CCNGG 4 cut(s) 865, 969, 1080, 1496
BmrI ACTGGG 1 cut(s) 1293
BmsI GCATC 3 cut(s) 229, 471, 942
BmuI ACTGGG 1 cut(s) 1293
BoxI GACNNNNGTC 1 cut(s) 879
BpiI GAAGAC 4 cut(s) 184, 246, 567, 1445
BplI GAGNNNNNCTC 2 cut(s) 764, 796
Bpu14I TTCGAA 1 cut(s) 613
BpuEI CTTGAG 2 cut(s) 1271, 1396
BpuMI CCSGG 2 cut(s) 865, 1080
Bsa29I ATCGAT 1 cut(s) 1157
BsaHI GRCGYC 1 cut(s) 1535
BsaI GGTCTC 2 cut(s) 1013, 1504
BsaJI CCNNGG 4 cut(s) 768, 1136, 1241, 1419
BsaXI ACNNNNNCTCC 2 cut(s) 274, 304
Bsc4I CCNNNNNNNGG 5 cut(s) 177, 216, 524, 850, 1243
Bse1I ACTGG 3 cut(s) 598, 979, 1299
Bse21I CCTNAGG 1 cut(s) 519
Bse3DI GCAATG 1 cut(s) 828
BseBI CCWGG 2 cut(s) 969, 1496
BseCI ATCGAT 1 cut(s) 1157
BseDI CCNNGG 4 cut(s) 768, 1136, 1241, 1419
BseGI GGATG 4 cut(s) 271, 598, 1021, 1435
BseLI CCNNNNNNNGG 5 cut(s) 177, 216, 524, 850, 1243
BseMI GCAATG 1 cut(s) 828
BseMII CTCAG 7 cut(s) 133, 378, 491, 510, 1505, 1515, 1600
BseNI ACTGG 3 cut(s) 598, 979, 1299
BseRI GAGGAG 5 cut(s) 48, 84, 87, 276, 279
BseXI GCAGC 1 cut(s) 1162
BsgI GTGCAG 1 cut(s) 1460
Bsh1285I CGRYCG 1 cut(s) 1157
BshFI GGCC 1 cut(s) 1380
BshNI GGYRCC 2 cut(s) 264, 1534
BshVI ATCGAT 1 cut(s) 1157
BsiEI CGRYCG 1 cut(s) 1157
BsiHKAI GWGCWC 1 cut(s) 29
BsiSI CCGG 2 cut(s) 865, 1079
BslI CCNNNNNNNGG 5 cut(s) 177, 216, 524, 850, 1243
BsmBI CGTCTC 6 cut(s) 122, 318, 650, 926, 1341, 1534
BsnI GGCC 1 cut(s) 1380
Bso31I GGTCTC 2 cut(s) 1013, 1504
Bsp119I TTCGAA 1 cut(s) 613
Bsp1286I GDGCHC 1 cut(s) 29
Bsp1407I TGTACA 1 cut(s) 1213
BspACI CCGC 8 cut(s) 135, 153, 293, 296, 299, 308, 822, 851
BspANI GGCC 1 cut(s) 1380
BspCNI CTCAG 7 cut(s) 132, 377, 490, 511, 1506, 1514, 1599
BspDI ATCGAT 1 cut(s) 1157
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 4 cut(s) 266, 1134, 1536, 1627
BspPI GGATC 6 cut(s) 427, 731, 1127, 1140, 1254, 1410
BspQI GCTCTTC 4 cut(s) 79, 162, 330, 498
BspT104I TTCGAA 1 cut(s) 613
BspT107I GGYRCC 2 cut(s) 264, 1534
BspTNI GGTCTC 2 cut(s) 1013, 1504
BsrBI CCGCTC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 828
BsrGI TGTACA 1 cut(s) 1213
BsrI ACTGG 3 cut(s) 598, 979, 1299
BssECI CCNNGG 4 cut(s) 768, 1136, 1241, 1419
BssNI GRCGYC 1 cut(s) 1535
BssT1I CCWWGG 2 cut(s) 1136, 1419
Bst2UI CCWGG 2 cut(s) 969, 1496
Bst4CI ACNGT 6 cut(s) 19, 65, 264, 908, 1545, 1597
Bst6I CTCTTC 7 cut(s) 79, 162, 321, 330, 498, 543, 1364
BstACI GRCGYC 1 cut(s) 1535
BstAUI TGTACA 1 cut(s) 1213
BstBI TTCGAA 1 cut(s) 613
BstC8I GCNNGC 2 cut(s) 931, 1257
BstDEI CTNAG 7 cut(s) 119, 364, 477, 519, 1501, 1514, 1586
BstDSI CCRYGG 1 cut(s) 1241
BstENI CCTNNNNNAGG 1 cut(s) 522
BstF5I GGATG 4 cut(s) 271, 598, 1021, 1435
BstH2I RGCGCY 1 cut(s) 1538
BstHHI GCGC 1 cut(s) 1537
BstMCI CGRYCG 1 cut(s) 1157
BstMWI GCNNNNNNNGC 7 cut(s) 143, 152, 173, 304, 307, 505, 1534
BstNI CCWGG 2 cut(s) 969, 1496
BstNSI RCATGY 1 cut(s) 494
BstPAI GACNNNNGTC 1 cut(s) 879
BstSCI CCNGG 4 cut(s) 863, 967, 1078, 1494
BstV1I GCAGC 1 cut(s) 1162
BstV2I GAAGAC 4 cut(s) 184, 246, 567, 1445
BstX2I RGATCY 4 cut(s) 419, 1132, 1246, 1415
BstYI RGATCY 4 cut(s) 419, 1132, 1246, 1415
Bsu15I ATCGAT 1 cut(s) 1157
Bsu36I CCTNAGG 1 cut(s) 519
BsuRI GGCC 1 cut(s) 1380
BsuTUI ATCGAT 1 cut(s) 1157
BtgI CCRYGG 1 cut(s) 1241
BtsCI GGATG 4 cut(s) 271, 598, 1021, 1435
BtsI GCAGTG 1 cut(s) 1610
BtsIMutI CAGTG 4 cut(s) 936, 986, 1306, 1610
Cac8I GCNNGC 2 cut(s) 931, 1257
CciI TCATGA 1 cut(s) 340
CfoI GCGC 1 cut(s) 1537
ClaI ATCGAT 1 cut(s) 1157
Csp6I GTAC 3 cut(s) 265, 1214, 1408
CviAII CATG 5 cut(s) 22, 341, 491, 808, 1004
CviQI GTAC 3 cut(s) 265, 1214, 1408
DdeI CTNAG 7 cut(s) 119, 364, 477, 519, 1501, 1514, 1586
DinI GGCGCC 1 cut(s) 1536
DraIII CACNNNGTG 1 cut(s) 1483
EaeI YGGCCR 1 cut(s) 1378
Eam1104I CTCTTC 7 cut(s) 79, 162, 321, 330, 498, 543, 1364
EarI CTCTTC 7 cut(s) 79, 162, 321, 330, 498, 543, 1364
EciI GGCGGA 1 cut(s) 282
Eco130I CCWWGG 2 cut(s) 1136, 1419
Eco31I GGTCTC 2 cut(s) 1013, 1504
Eco32I GATATC 1 cut(s) 1197
Eco57I CTGAAG 1 cut(s) 579
Eco81I CCTNAGG 1 cut(s) 519
EcoNI CCTNNNNNAGG 1 cut(s) 522
EcoRII CCWGG 2 cut(s) 967, 1494
EcoRV GATATC 1 cut(s) 1197
EcoT14I CCWWGG 2 cut(s) 1136, 1419
EgeI GGCGCC 1 cut(s) 1536
EheI GGCGCC 1 cut(s) 1536
ErhI CCWWGG 2 cut(s) 1136, 1419
Esp3I CGTCTC 6 cut(s) 122, 318, 650, 926, 1341, 1534
FaeI CATG 5 cut(s) 25, 344, 494, 811, 1007
FatI CATG 5 cut(s) 21, 340, 490, 807, 1003
FbaI TGATCA 1 cut(s) 1168
Fnu4HI GCNGC 4 cut(s) 296, 299, 308, 1151
FokI GGATG 4 cut(s) 258, 585, 1008, 1442
Fsp4HI GCNGC 4 cut(s) 296, 299, 308, 1151
FspBI CTAG 1 cut(s) 1137
GlaI GCGC 1 cut(s) 1536
GluI GCNGC 4 cut(s) 296, 299, 308, 1151
HaeII RGCGCY 1 cut(s) 1538
HaeIII GGCC 1 cut(s) 1380
HapII CCGG 2 cut(s) 865, 1079
HhaI GCGC 1 cut(s) 1537
Hin1I GRCGYC 1 cut(s) 1535
Hin1II CATG 5 cut(s) 25, 344, 494, 811, 1007
Hin6I GCGC 1 cut(s) 1535
HinP1I GCGC 1 cut(s) 1535
HincII GTYRAC 1 cut(s) 1295
HindII GTYRAC 1 cut(s) 1295
HindIII AAGCTT 2 cut(s) 1322, 1445
HinfI GANTC 6 cut(s) 102, 394, 533, 615, 635, 868
HpaII CCGG 2 cut(s) 865, 1079
HphI GGTGA 6 cut(s) 7, 53, 189, 539, 692, 968
Hpy166II GTNNAC 2 cut(s) 488, 1295
Hpy188I TCNGA 8 cut(s) 367, 480, 559, 745, 772, 947, 1515, 1589
Hpy8I GTNNAC 2 cut(s) 488, 1295
Hpy99I CGWCG 4 cut(s) 176, 307, 444, 878
HpyAV CCTTC 5 cut(s) 483, 518, 612, 750, 1379
HpyCH4III ACNGT 6 cut(s) 19, 65, 264, 908, 1545, 1597
HpyCH4IV ACGT 3 cut(s) 439, 1053, 1406
HpyCH4V TGCA 5 cut(s) 933, 1031, 1150, 1477, 1554
HpyF10VI GCNNNNNNNGC 7 cut(s) 143, 152, 173, 304, 307, 505, 1534
HpyF3I CTNAG 7 cut(s) 119, 364, 477, 519, 1501, 1514, 1586
HpySE526I ACGT 3 cut(s) 439, 1053, 1406
Hsp92I GRCGYC 1 cut(s) 1535
Hsp92II CATG 5 cut(s) 25, 344, 494, 811, 1007
HspAI GCGC 1 cut(s) 1535
KasI GGCGCC 1 cut(s) 1534
KpnI GGTACC 1 cut(s) 268
Ksp22I TGATCA 1 cut(s) 1168
LguI GCTCTTC 4 cut(s) 79, 162, 330, 498
Lsp1109I GCAGC 1 cut(s) 1162
LweI GCATC 3 cut(s) 229, 471, 942
MaeI CTAG 1 cut(s) 1137
MaeII ACGT 3 cut(s) 439, 1053, 1406
MaeIII GTNAC 2 cut(s) 280, 578
MbiI CCGCTC 1 cut(s) 155
MfeI CAATTG 1 cut(s) 276
MflI RGATCY 4 cut(s) 419, 1132, 1246, 1415
MhlI GDGCHC 1 cut(s) 29
MlsI TGGCCA 1 cut(s) 1380
MluCI AATT 8 cut(s) 9, 276, 344, 549, 1044, 1329, 1522, 1617
MluNI TGGCCA 1 cut(s) 1380
Mly113I GGCGCC 1 cut(s) 1535
MlyI GAGTC 1 cut(s) 644
Mox20I TGGCCA 1 cut(s) 1380
MscI TGGCCA 1 cut(s) 1380
MseI TTAA 6 cut(s) 8, 228, 725, 990, 1065, 1592
MslI CAYNNNNRTG 1 cut(s) 20
Msp20I TGGCCA 1 cut(s) 1380
MspI CCGG 2 cut(s) 865, 1079
MspR9I CCNGG 4 cut(s) 865, 969, 1080, 1496
MunI CAATTG 1 cut(s) 276
MvaI CCWGG 2 cut(s) 969, 1496
MwoI GCNNNNNNNGC 7 cut(s) 143, 152, 173, 304, 307, 505, 1534
NarI GGCGCC 1 cut(s) 1535
NciI CCSGG 2 cut(s) 865, 1080
NlaIII CATG 5 cut(s) 25, 344, 494, 811, 1007
NlaIV GGNNCC 4 cut(s) 266, 1134, 1536, 1627
NmeAIII GCCGAG 1 cut(s) 1563
NspI RCATGY 1 cut(s) 494
NspV TTCGAA 1 cut(s) 613
PagI TCATGA 1 cut(s) 340
PciI ACATGT 1 cut(s) 490
PciSI GCTCTTC 4 cut(s) 79, 162, 330, 498
PcsI WCGNNNNNNNCGW 3 cut(s) 121, 619, 882
PfeI GAWTC 5 cut(s) 102, 394, 533, 615, 868
PfoI TCCNGGA 2 cut(s) 863, 1078
PkrI GCNGC 4 cut(s) 297, 300, 309, 1152
Ple19I CGATCG 1 cut(s) 1157
PleI GAGTC 1 cut(s) 643
PluTI GGCGCC 1 cut(s) 1538
PpsI GAGTC 1 cut(s) 643
PscI ACATGT 1 cut(s) 490
PshAI GACNNNNGTC 1 cut(s) 879
Psp6I CCWGG 2 cut(s) 967, 1494
PspGI CCWGG 2 cut(s) 967, 1494
PspN4I GGNNCC 4 cut(s) 266, 1134, 1536, 1627
PsrI GAACNNNNNNTAC 2 cut(s) 248, 280
PsuI RGATCY 4 cut(s) 419, 1132, 1246, 1415
PvuI CGATCG 1 cut(s) 1157
RsaI GTAC 3 cut(s) 266, 1215, 1409
RsaNI GTAC 3 cut(s) 265, 1214, 1408
RseI CAYNNNNRTG 1 cut(s) 20
SapI GCTCTTC 4 cut(s) 79, 162, 330, 498
SaqAI TTAA 6 cut(s) 8, 228, 725, 990, 1065, 1592
SatI GCNGC 4 cut(s) 296, 299, 308, 1151
SchI GAGTC 1 cut(s) 644
ScrFI CCNGG 4 cut(s) 865, 969, 1080, 1496
SduI GDGCHC 1 cut(s) 29
SfaNI GCATC 3 cut(s) 229, 471, 942
SfoI GGCGCC 1 cut(s) 1536
SfuI TTCGAA 1 cut(s) 613
SmiMI CAYNNNNRTG 1 cut(s) 20
SmlI CTYRAG 2 cut(s) 1250, 1411
SmoI CTYRAG 2 cut(s) 1250, 1411
Sse9I AATT 8 cut(s) 9, 276, 344, 549, 1044, 1329, 1522, 1617
SsiI CCGC 8 cut(s) 135, 153, 293, 296, 299, 308, 822, 851
SspDI GGCGCC 1 cut(s) 1534
SspMI CTAG 1 cut(s) 1137
StyD4I CCNGG 4 cut(s) 863, 967, 1078, 1494
StyI CCWWGG 2 cut(s) 1136, 1419
TaaI ACNGT 6 cut(s) 19, 65, 264, 908, 1545, 1597
TaiI ACGT 3 cut(s) 442, 1056, 1409
TaqI TCGA 5 cut(s) 100, 336, 613, 885, 1157
TasI AATT 8 cut(s) 9, 276, 344, 549, 1044, 1329, 1522, 1617
TatI WGTACW 1 cut(s) 1213
TauI GCSGC 3 cut(s) 298, 301, 310
TfiI GAWTC 5 cut(s) 102, 394, 533, 615, 868
Tru1I TTAA 6 cut(s) 8, 228, 725, 990, 1065, 1592
Tru9I TTAA 6 cut(s) 8, 228, 725, 990, 1065, 1592
TscAI CASTG 4 cut(s) 943, 986, 1306, 1610
TseI GCWGC 1 cut(s) 1150
TspDTI ATGAA 6 cut(s) 22, 39, 357, 407, 695, 1037
TspGWI ACGGA 3 cut(s) 95, 160, 318
TspRI CASTG 4 cut(s) 943, 986, 1306, 1610
XagI CCTNNNNNAGG 1 cut(s) 522
XapI RAATTY 1 cut(s) 344
XceI RCATGY 1 cut(s) 494
XmaJI CCTAGG 1 cut(s) 1136
XspI CTAG 1 cut(s) 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.